MD02G1289000.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001233
Physical Location & Seq
Forward (+)
34533143 .. 34533784
642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1289000.v1.1.491

Sequence Viewer

Length: 642 bp
ATGTCTTGCCCGCATGCTGCTGGTGTCGCTGCGTATATCAAAACATTCCACCCCAACTGGTCTCCAGCTGCCATCAAATCATCTATCATGACTACGGCTGGGCCTGCGAATGGCACTGACGATTCCTACACGCCCGGTGTATTTTCCTATGGTTCTGGGCATATCAATCCTCTAAAAGCTTTAAATCCGGGCCTTGTGTATGAAACTTCTAAGGATGATTACATTAAGTTGCTCTGCTCGATCTTGGACGAGTCCAAAGTTCGACTTATTTCTGGAGACAAACGCCCTTGCCCTGCAAAGAATGACAAAATATTGGCCAAAGATCTCAACTACCCTTCAATGGCTGTTAACATTCTTGCAGAGACTACGTTTTCGATTCAGTTTCAAAGAACAGTTAAAAATGTGGGCCATGCAAACTCCATTTACAAGGCAAAGACCATCTCAAAACCAGGAATTAACATCAAGGTGGTGCCTCAAGTTCTTTCCTTCAAGTCTCTGAATGAGAAAAAGACCTTCAATGTGACAGTTTTCGGAAGAAGTATACCATTACTTACGCGTGTATCTGCGTCGTTGGTGTGGACGGATGGTACTCACAGTGTTCGAAGTCCAATTCTTGTAAGCGCCACCAACTACAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

214

Amino Acids

23.03

Weight (kDa)

9.61

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 1 - 42 1.1e-07 Subtilase family
fn3_6 PF17766 108 - 206 8.6e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 469
AccI GTMKAC 1 cut(s) 541
AccII CGCG 1 cut(s) 556
AciI CCGC 1 cut(s) 11
AcoI YGGCCR 1 cut(s) 315
AfaI GTAC 1 cut(s) 589
AfiI CCNNNNNNNGG 2 cut(s) 110, 340
AflIII ACRYGT 1 cut(s) 554
AgsI TTSAA 4 cut(s) 339, 386, 490, 517
AjnI CCWGG 1 cut(s) 448
AluBI AGCT 3 cut(s) 68, 179, 636
AluI AGCT 3 cut(s) 68, 179, 636
Alw26I GTCTC 4 cut(s) 66, 270, 356, 498
AoxI GGCC 4 cut(s) 101, 190, 315, 406
ApeKI GCWGC 3 cut(s) 17, 29, 68
ArsI GACNNNNNNTTYG 2 cut(s) 355, 387
AspLEI GCGC 1 cut(s) 623
AspS9I GGNCC 3 cut(s) 101, 190, 406
AsuC2I CCSGG 2 cut(s) 135, 189
AsuII TTCGAA 1 cut(s) 601
BaeI ACNNNNGTAYC 2 cut(s) 543, 576
BalI TGGCCA 1 cut(s) 317
BanI GGYRCC 1 cut(s) 469
BbvI GCAGC 3 cut(s) 4, 16, 55
BccI CCATC 3 cut(s) 80, 446, 578
BceAI ACGGC 1 cut(s) 111
BciT130I CCWGG 1 cut(s) 450
BcnI CCSGG 2 cut(s) 135, 189
BcoDI GTCTC 4 cut(s) 66, 270, 356, 498
BfmI CTRYAG 1 cut(s) 631
BfoI RGCGCY 1 cut(s) 624
BglII AGATCT 1 cut(s) 322
BisI GCNGC 3 cut(s) 18, 30, 69
BlsI GCNGC 3 cut(s) 19, 31, 70
Bme1390I CCNGG 3 cut(s) 135, 189, 450
BmgT120I GGNCC 3 cut(s) 101, 190, 406
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 3 cut(s) 135, 189, 450
BpmI CTGGAG 2 cut(s) 48, 294
Bpu14I TTCGAA 1 cut(s) 601
BpuEI CTTGAG 1 cut(s) 459
BpuMI CCSGG 2 cut(s) 135, 189
BsaI GGTCTC 1 cut(s) 66
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 340
Bse1I ACTGG 1 cut(s) 62
BseBI CCWGG 1 cut(s) 450
BseGI GGATG 2 cut(s) 220, 589
BseLI CCNNNNNNNGG 2 cut(s) 110, 340
BseNI ACTGG 1 cut(s) 62
BseXI GCAGC 3 cut(s) 4, 16, 55
BseYI CCCAGC 1 cut(s) 98
Bsh1236I CGCG 1 cut(s) 556
BshFI GGCC 4 cut(s) 103, 192, 317, 408
BshNI GGYRCC 1 cut(s) 469
BsiSI CCGG 2 cut(s) 135, 188
BslI CCNNNNNNNGG 2 cut(s) 110, 340
BsmAI GTCTC 4 cut(s) 66, 270, 356, 498
BsnI GGCC 4 cut(s) 103, 192, 317, 408
Bso31I GGTCTC 1 cut(s) 66
Bsp119I TTCGAA 1 cut(s) 601
Bsp143I GATC 2 cut(s) 240, 322
BspACI CCGC 1 cut(s) 11
BspANI GGCC 4 cut(s) 103, 192, 317, 408
BspFNI CGCG 1 cut(s) 556
BspHI TCATGA 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 471
BspT104I TTCGAA 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 469
BspTNI GGTCTC 1 cut(s) 66
BsrI ACTGG 1 cut(s) 62
BssMI GATC 2 cut(s) 240, 322
BssNAI GTATAC 1 cut(s) 542
Bst1107I GTATAC 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 3 cut(s) 394, 526, 596
BstBI TTCGAA 1 cut(s) 601
BstC8I GCNNGC 3 cut(s) 11, 15, 105
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 2 cut(s) 220, 589
BstFNI CGCG 1 cut(s) 556
BstH2I RGCGCY 1 cut(s) 624
BstHHI GCGC 1 cut(s) 623
BstKTI GATC 2 cut(s) 243, 325
BstMAI GTCTC 4 cut(s) 66, 270, 356, 498
BstMBI GATC 2 cut(s) 240, 322
BstMWI GCNNNNNNNGC 2 cut(s) 26, 104
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 1 cut(s) 17
BstSCI CCNGG 3 cut(s) 133, 187, 448
BstSFI CTRYAG 1 cut(s) 631
BstUI CGCG 1 cut(s) 556
BstV1I GCAGC 3 cut(s) 4, 16, 55
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BstZ17I GTATAC 1 cut(s) 542
BsuRI GGCC 4 cut(s) 103, 192, 317, 408
BtsCI GGATG 2 cut(s) 220, 589
BtsIMutI CAGTG 2 cut(s) 114, 601
Cac8I GCNNGC 3 cut(s) 11, 15, 105
CciI TCATGA 1 cut(s) 87
CfoI GCGC 1 cut(s) 623
Cfr13I GGNCC 3 cut(s) 101, 190, 406
CseI GACGC 1 cut(s) 555
Csp6I GTAC 1 cut(s) 588
CviAII CATG 3 cut(s) 14, 88, 410
CviJI RGCY 9 cut(s) 68, 98, 103, 179, 192, 317, 344, 408, 636
CviKI_1 RGCY 9 cut(s) 68, 98, 103, 179, 192, 317, 344, 408, 636
CviQI GTAC 1 cut(s) 588
DdeI CTNAG 1 cut(s) 210
DpnI GATC 2 cut(s) 242, 324
DpnII GATC 2 cut(s) 240, 322
DraI TTTAAA 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 448
FaeI CATG 3 cut(s) 17, 91, 413
FaiI YATR 8 cut(s) 15, 36, 89, 150, 162, 201, 411, 542
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FatI CATG 3 cut(s) 13, 87, 409
FauI CCCGC 1 cut(s) 18
FblI GTMKAC 1 cut(s) 541
Fnu4HI GCNGC 3 cut(s) 18, 30, 69
FokI GGATG 2 cut(s) 227, 596
Fsp4HI GCNGC 3 cut(s) 18, 30, 69
GlaI GCGC 1 cut(s) 622
GluI GCNGC 3 cut(s) 18, 30, 69
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 2 cut(s) 48, 294
HaeII RGCGCY 1 cut(s) 624
HaeIII GGCC 4 cut(s) 103, 192, 317, 408
HapII CCGG 2 cut(s) 135, 188
HgaI GACGC 1 cut(s) 555
HhaI GCGC 1 cut(s) 623
Hin1II CATG 3 cut(s) 17, 91, 413
Hin6I GCGC 1 cut(s) 621
HinP1I GCGC 1 cut(s) 621
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 3 cut(s) 122, 251, 376
HpaI GTTAAC 1 cut(s) 349
HpaII CCGG 2 cut(s) 135, 188
Hpy166II GTNNAC 3 cut(s) 349, 542, 579
Hpy188I TCNGA 2 cut(s) 498, 533
Hpy188III TCNNGA 3 cut(s) 88, 273, 639
Hpy8I GTNNAC 3 cut(s) 349, 542, 579
Hpy99I CGWCG 1 cut(s) 571
HpyAV CCTTC 3 cut(s) 345, 496, 523
HpyCH4III ACNGT 3 cut(s) 394, 526, 596
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 3 cut(s) 296, 359, 413
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 104
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 3 cut(s) 17, 91, 413
HspAI GCGC 1 cut(s) 621
KspAI GTTAAC 1 cut(s) 349
Kzo9I GATC 2 cut(s) 240, 322
Lsp1109I GCAGC 3 cut(s) 4, 16, 55
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 1 cut(s) 520
MalI GATC 2 cut(s) 242, 324
MboI GATC 2 cut(s) 240, 322
MboII GAAGA 1 cut(s) 546
MflI RGATCY 1 cut(s) 322
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 2 cut(s) 453, 609
MluI ACGCGT 1 cut(s) 554
MluNI TGGCCA 1 cut(s) 317
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 2 cut(s) 180, 483
Mox20I TGGCCA 1 cut(s) 317
MscI TGGCCA 1 cut(s) 317
MseI TTAA 5 cut(s) 182, 225, 348, 396, 456
MslI CAYNNNNRTG 1 cut(s) 464
Msp20I TGGCCA 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 2 cut(s) 135, 188
MspR9I CCNGG 3 cut(s) 135, 189, 450
MvaI CCWGG 1 cut(s) 450
MvnI CGCG 1 cut(s) 556
MwoI GCNNNNNNNGC 2 cut(s) 26, 104
NciI CCSGG 2 cut(s) 135, 189
NdeII GATC 2 cut(s) 240, 322
NlaIII CATG 3 cut(s) 17, 91, 413
NlaIV GGNNCC 1 cut(s) 471
NmuCI GTSAC 1 cut(s) 520
NspI RCATGY 1 cut(s) 17
NspV TTCGAA 1 cut(s) 601
PaeI GCATGC 1 cut(s) 17
PagI TCATGA 1 cut(s) 87
PfeI GAWTC 2 cut(s) 122, 376
PkrI GCNGC 3 cut(s) 19, 31, 70
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 448
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 3 cut(s) 101, 190, 406
PsuI RGATCY 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 1 cut(s) 589
RsaNI GTAC 1 cut(s) 588
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 5 cut(s) 182, 225, 348, 396, 456
SatI GCNGC 3 cut(s) 18, 30, 69
Sau3AI GATC 2 cut(s) 240, 322
Sau96I GGNCC 3 cut(s) 101, 190, 406
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 3 cut(s) 135, 189, 450
SetI ASST 6 cut(s) 70, 181, 371, 468, 515, 638
SfcI CTRYAG 1 cut(s) 631
SfuI TTCGAA 1 cut(s) 601
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
SphI GCATGC 1 cut(s) 17
Sse9I AATT 2 cut(s) 453, 609
SsiI CCGC 1 cut(s) 11
SspI AATATT 1 cut(s) 312
StyD4I CCNGG 3 cut(s) 133, 187, 448
TaaI ACNGT 3 cut(s) 394, 526, 596
TaiI ACGT 1 cut(s) 371
TaqI TCGA 4 cut(s) 239, 262, 374, 601
TasI AATT 2 cut(s) 453, 609
TfiI GAWTC 2 cut(s) 122, 376
Tru1I TTAA 5 cut(s) 182, 225, 348, 396, 456
Tru9I TTAA 5 cut(s) 182, 225, 348, 396, 456
TscAI CASTG 2 cut(s) 121, 601
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 3 cut(s) 17, 29, 68
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 1 cut(s) 216
TspGWI ACGGA 1 cut(s) 596
TspRI CASTG 2 cut(s) 121, 601
XceI RCATGY 1 cut(s) 17
XmiI GTMKAC 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.