Rh1DG059800

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001233
Physical Location & Seq
Reverse (-)
10328875 .. 10329399
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG059800.1

Sequence Viewer

Length: 525 bp
ATGAATATTACAGAAAATTTCCCAGGTGAATTTGCTTTTGGATCTGGACATATCAATCCTTTAAAAGCTATAAACCCGGGGCTTGAGTATGAAGCTTCTAAAAAAGATTACACAAAGTTGCTCTGCATGTTGTTTGATGAGACCAAAGTGAGACTTATATCAGGAGAAAACAGCACTTGTTCTGTAGGCTCTGACACAGGATCGCCAAAGGATTACAATTACCCTTCAATGGTAGCTATTGTTGCACCAATGACACCCTTTACGGTTAGAAGTCATAGAAGAGCTAAAAATGTTGACCATGCAAACTCCACTTACCTGGCTAAAATCTGGTCAAATTCTCAAGTCGACATCAAATTCATGCCTGAAATTCATGCCTTCAAGTCATTGAATGAGAAGAAGACTTTCGAAGTGACCATTATTGGAAGAGGTATACCAGATGGATCCCATGTATCTGCTTCACTGCTCTGGTCTGATGGTACTCATAGCGTTAGAAGTCCAATTCTTGTACAAAGCACAGCAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

174

Amino Acids

19.12

Weight (kDa)

7.81

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 71 - 169 2.8e-20 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 345, 430
AclWI GGATC 4 cut(s) 49, 208, 435, 448
AcsI RAATTY 5 cut(s) 16, 29, 334, 353, 366
AfaI GTAC 2 cut(s) 478, 507
AfiI CCNNNNNNNGG 1 cut(s) 229
AgsI TTSAA 3 cut(s) 228, 379, 388
AjnI CCWGG 2 cut(s) 22, 315
AjuI GAANNNNNNNTTGG 2 cut(s) 21, 53
AluBI AGCT 4 cut(s) 68, 95, 236, 284
AluI AGCT 4 cut(s) 68, 95, 236, 284
Alw26I GTCTC 2 cut(s) 134, 145
AlwI GGATC 4 cut(s) 49, 208, 435, 448
Ama87I CYCGRG 1 cut(s) 76
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 5 cut(s) 16, 29, 334, 353, 366
Asp700I GAANNNNTTC 1 cut(s) 401
AsuC2I CCSGG 2 cut(s) 77, 78
AsuHPI GGTGA 1 cut(s) 38
AsuII TTCGAA 1 cut(s) 405
AvaI CYCGRG 1 cut(s) 76
BamHI GGATCC 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 404
BccI CCATC 2 cut(s) 431, 467
BciT130I CCWGG 2 cut(s) 24, 317
BcnI CCSGG 2 cut(s) 77, 78
BcoDI GTCTC 2 cut(s) 134, 145
BfmI CTRYAG 1 cut(s) 183
BisI GCNGC 1 cut(s) 519
BlsI GCNGC 1 cut(s) 520
Bme1390I CCNGG 4 cut(s) 24, 77, 78, 317
BmeT110I CYCGRG 1 cut(s) 76
BmiI GGNNCC 1 cut(s) 442
BmrFI CCNGG 4 cut(s) 24, 77, 78, 317
BpiI GAAGAC 1 cut(s) 404
Bpu14I TTCGAA 1 cut(s) 405
BpuEI CTTGAG 2 cut(s) 104, 324
BpuMI CCSGG 2 cut(s) 77, 78
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 3 cut(s) 22, 76, 77
Bsc4I CCNNNNNNNGG 1 cut(s) 229
BseBI CCWGG 2 cut(s) 24, 317
BseDI CCNNGG 3 cut(s) 22, 76, 77
BseLI CCNNNNNNNGG 1 cut(s) 229
BsiHKCI CYCGRG 1 cut(s) 76
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 2 cut(s) 134, 145
Bso31I GGTCTC 1 cut(s) 134
BsoBI CYCGRG 1 cut(s) 76
Bsp119I TTCGAA 1 cut(s) 405
Bsp1407I TGTACA 1 cut(s) 505
Bsp143I GATC 3 cut(s) 41, 200, 440
BspLI GGNNCC 1 cut(s) 442
BspPI GGATC 4 cut(s) 49, 208, 435, 448
BspQI GCTCTTC 1 cut(s) 274
BspT104I TTCGAA 1 cut(s) 405
BspTNI GGTCTC 1 cut(s) 134
BsrGI TGTACA 1 cut(s) 505
BssECI CCNNGG 3 cut(s) 22, 76, 77
BssMI GATC 3 cut(s) 41, 200, 440
BssNAI GTATAC 1 cut(s) 431
Bst1107I GTATAC 1 cut(s) 431
Bst2UI CCWGG 2 cut(s) 24, 317
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 2 cut(s) 274, 418
BstAUI TGTACA 1 cut(s) 505
BstBI TTCGAA 1 cut(s) 405
BstKTI GATC 3 cut(s) 44, 203, 443
BstMAI GTCTC 2 cut(s) 134, 145
BstMBI GATC 3 cut(s) 41, 200, 440
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstNI CCWGG 2 cut(s) 24, 317
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 4 cut(s) 22, 75, 76, 315
BstSFI CTRYAG 1 cut(s) 183
BstV2I GAAGAC 1 cut(s) 404
BstX2I RGATCY 2 cut(s) 41, 440
BstXI CCANNNNNNTGG 1 cut(s) 316
BstYI RGATCY 2 cut(s) 41, 440
BstZ17I GTATAC 1 cut(s) 431
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 1 cut(s) 458
Cfr9I CCCGGG 1 cut(s) 76
Csp6I GTAC 2 cut(s) 477, 506
CviAII CATG 5 cut(s) 127, 299, 358, 371, 446
CviJI RGCY 7 cut(s) 68, 82, 95, 189, 236, 284, 320
CviKI_1 RGCY 7 cut(s) 68, 82, 95, 189, 236, 284, 320
CviQI GTAC 2 cut(s) 477, 506
DpnI GATC 3 cut(s) 43, 202, 442
DpnII GATC 3 cut(s) 41, 200, 440
DraI TTTAAA 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 274, 418
EarI CTCTTC 2 cut(s) 274, 418
Eco31I GGTCTC 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 76
EcoRII CCWGG 2 cut(s) 22, 315
FaeI CATG 5 cut(s) 130, 302, 361, 374, 449
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FatI CATG 5 cut(s) 126, 298, 357, 370, 445
FblI GTMKAC 2 cut(s) 345, 430
Fnu4HI GCNGC 1 cut(s) 519
Fsp4HI GCNGC 1 cut(s) 519
GluI GCNGC 1 cut(s) 519
HapII CCGG 1 cut(s) 77
Hin1II CATG 5 cut(s) 130, 302, 361, 374, 449
HincII GTYRAC 2 cut(s) 295, 346
HindII GTYRAC 2 cut(s) 295, 346
HindIII AAGCTT 1 cut(s) 93
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 3 cut(s) 295, 346, 431
Hpy188I TCNGA 2 cut(s) 193, 472
Hpy188III TCNNGA 2 cut(s) 45, 162
Hpy8I GTNNAC 3 cut(s) 295, 346, 431
HpyAV CCTTC 2 cut(s) 234, 385
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 3 cut(s) 126, 245, 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
Hsp92II CATG 5 cut(s) 130, 302, 361, 374, 449
Kzo9I GATC 3 cut(s) 41, 200, 440
LguI GCTCTTC 1 cut(s) 274
MaeIII GTNAC 1 cut(s) 409
MalI GATC 3 cut(s) 43, 202, 442
MboI GATC 3 cut(s) 41, 200, 440
MboII GAAGA 4 cut(s) 291, 406, 409, 435
MflI RGATCY 2 cut(s) 41, 440
MluCI AATT 7 cut(s) 16, 29, 217, 334, 353, 366, 498
MnlI CCTC 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 401
MseI TTAA 1 cut(s) 62
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 4 cut(s) 24, 77, 78, 317
MvaI CCWGG 2 cut(s) 24, 317
MwoI GCNNNNNNNGC 1 cut(s) 242
NciI CCSGG 2 cut(s) 77, 78
NdeII GATC 3 cut(s) 41, 200, 440
NlaIII CATG 5 cut(s) 130, 302, 361, 374, 449
NlaIV GGNNCC 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 1 cut(s) 130
NspV TTCGAA 1 cut(s) 405
PciSI GCTCTTC 1 cut(s) 274
PdmI GAANNNNTTC 1 cut(s) 401
PkrI GCNGC 1 cut(s) 520
Psp6I CCWGG 2 cut(s) 22, 315
PspGI CCWGG 2 cut(s) 22, 315
PspN4I GGNNCC 1 cut(s) 442
PsuI RGATCY 2 cut(s) 41, 440
RsaI GTAC 2 cut(s) 478, 507
RsaNI GTAC 2 cut(s) 477, 506
SalI GTCGAC 1 cut(s) 344
SapI GCTCTTC 1 cut(s) 274
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 3 cut(s) 41, 200, 440
ScrFI CCNGG 4 cut(s) 24, 77, 78, 317
SetI ASST 7 cut(s) 28, 70, 97, 238, 286, 318, 430
SfcI CTRYAG 1 cut(s) 183
SfuI TTCGAA 1 cut(s) 405
SmaI CCCGGG 1 cut(s) 78
SmlI CTYRAG 2 cut(s) 83, 339
SmoI CTYRAG 2 cut(s) 83, 339
Sse9I AATT 7 cut(s) 16, 29, 217, 334, 353, 366, 498
SspI AATATT 1 cut(s) 7
StyD4I CCNGG 4 cut(s) 22, 75, 76, 315
TaaI ACNGT 1 cut(s) 265
TaqI TCGA 2 cut(s) 345, 405
TasI AATT 7 cut(s) 16, 29, 217, 334, 353, 366, 498
TatI WGTACW 1 cut(s) 505
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 518
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 4 cut(s) 17, 105, 346, 359
TspMI CCCGGG 1 cut(s) 76
TspRI CASTG 1 cut(s) 465
XapI RAATTY 5 cut(s) 16, 29, 334, 353, 366
XceI RCATGY 1 cut(s) 130
XmaI CCCGGG 1 cut(s) 76
XmiI GTMKAC 2 cut(s) 345, 430
XmnI GAANNNNTTC 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.