Rh1DG060300

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001233
Physical Location & Seq
Reverse (-)
10380391 .. 10384996
4606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG060300.1

Sequence Viewer

Length: 552 bp
ATGAATGTTTCTGACAATAGCAGTGCCGCTGGTGAATTTGCTTATGGATCTGGACATATCAATCCTGTCAAAGCTATAGACCCAGGGCTTGTGTATGAAGCTTCTAAGGAAGACTACATAAAGTTGCTATGCTCGGTCTTGGATCAGCCCGATGTTAGACTTATATCAGGAGATAACAGCACTTGCTCTACAGGCTCTGACAAAGGATCTTTACAGGATCACAATTACCCTTCACTAGCAGCCGTTGTCACACCAAACAAATCTTTTTCGTTGAAATTTAACAGAAAAGTTAAGAATGTTGGCCTTGCAAACTCCACTTACAAGGCCACAATATTCGCCAACTCTACTCAAGTTGACGTCAAAGTGGTGCCTCAAGTTCTTTCCTTCAAGTCCTTGAATGAGGAGAAGGCTTTTGATGTGACAGTTGCCGGAAAAGGTTTGCCAGATGAAGTACAATCACATGTGTCTGCATCGCTGGTGTGGTCTGATGGAATTCATAGCTTAGCTGACCAGATGGAAGAAGATGCTTCAGTAACTAACGCTGAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

183

Amino Acids

19.51

Weight (kDa)

4.88

Isoelectric Point (pI)

20.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 73 - 167 9.7e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 360
AccB1I GGYRCC 1 cut(s) 367
AciI CCGC 1 cut(s) 27
AclWI GGATC 4 cut(s) 55, 150, 214, 225
AcsI RAATTY 3 cut(s) 35, 275, 492
AcuI CTGAAG 1 cut(s) 513
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 1 cut(s) 453
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 3 cut(s) 274, 388, 397
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 4 cut(s) 74, 101, 501, 506
AluI AGCT 4 cut(s) 74, 101, 501, 506
AlwI GGATC 4 cut(s) 55, 150, 214, 225
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 2 cut(s) 301, 324
ApeKI GCWGC 1 cut(s) 239
ApoI RAATTY 3 cut(s) 35, 275, 492
AsuHPI GGTGA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 367
BbsI GAAGAC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 251
BccI CCATC 2 cut(s) 482, 508
BceAI ACGGC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 84
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 2 cut(s) 75, 189
BisI GCNGC 2 cut(s) 27, 240
BlpI GCTNAGC 1 cut(s) 502
BlsI GCNGC 2 cut(s) 28, 241
Bme1390I CCNGG 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 2 cut(s) 479, 514
BpiI GAAGAC 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 502
BpuEI CTTGAG 2 cut(s) 333, 357
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 82, 83
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 82, 83
BseMII CTCAG 1 cut(s) 534
BseRI GAGGAG 1 cut(s) 416
BseXI GCAGC 1 cut(s) 251
BshFI GGCC 2 cut(s) 303, 326
BshNI GGYRCC 1 cut(s) 367
BsiSI CCGG 1 cut(s) 429
BsnI GGCC 2 cut(s) 303, 326
Bsp143I GATC 4 cut(s) 47, 142, 206, 217
Bsp1720I GCTNAGC 1 cut(s) 502
BspACI CCGC 1 cut(s) 27
BspANI GGCC 2 cut(s) 303, 326
BspCNI CTCAG 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 369
BspPI GGATC 4 cut(s) 55, 150, 214, 225
BspT107I GGYRCC 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 82, 83
BssMI GATC 4 cut(s) 47, 142, 206, 217
BssNI GRCGYC 1 cut(s) 357
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 424
BstACI GRCGYC 1 cut(s) 357
BstDEI CTNAG 3 cut(s) 105, 502, 543
BstKTI GATC 4 cut(s) 50, 145, 209, 220
BstMBI GATC 4 cut(s) 47, 142, 206, 217
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 82
BstSFI CTRYAG 2 cut(s) 75, 189
BstV1I GCAGC 1 cut(s) 251
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 2 cut(s) 47, 206
BstYI RGATCY 2 cut(s) 47, 206
BsuRI GGCC 2 cut(s) 303, 326
BtgZI GCGATG 1 cut(s) 456
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 1 cut(s) 28
CaiI CAGNNNCTG 1 cut(s) 197
Csp6I GTAC 1 cut(s) 452
CviAII CATG 1 cut(s) 461
CviQI GTAC 1 cut(s) 452
DdeI CTNAG 3 cut(s) 105, 502, 543
DpnI GATC 4 cut(s) 49, 144, 208, 219
DpnII GATC 4 cut(s) 47, 142, 206, 217
Eco57I CTGAAG 1 cut(s) 513
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 82
FaeI CATG 1 cut(s) 464
FaiI YATR 9 cut(s) 45, 57, 77, 96, 119, 130, 164, 462, 498
FatI CATG 1 cut(s) 460
Fnu4HI GCNGC 2 cut(s) 27, 240
Fsp4HI GCNGC 2 cut(s) 27, 240
FspBI CTAG 1 cut(s) 236
GluI GCNGC 2 cut(s) 27, 240
HaeIII GGCC 2 cut(s) 303, 326
HapII CCGG 1 cut(s) 429
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 1 cut(s) 464
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 99
HpaII CCGG 1 cut(s) 429
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 3 cut(s) 13, 199, 487
Hpy188III TCNNGA 2 cut(s) 51, 168
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 3 cut(s) 240, 394, 400
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 2 cut(s) 308, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 3 cut(s) 105, 502, 543
HpySE526I ACGT 1 cut(s) 357
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 1 cut(s) 464
Kzo9I GATC 4 cut(s) 47, 142, 206, 217
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 2 cut(s) 479, 514
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 3 cut(s) 247, 418, 532
MalI GATC 4 cut(s) 49, 144, 208, 219
MboI GATC 4 cut(s) 47, 142, 206, 217
MboII GAAGA 3 cut(s) 122, 530, 533
MflI RGATCY 2 cut(s) 47, 206
MluCI AATT 5 cut(s) 35, 223, 275, 492, 547
MnlI CCTC 2 cut(s) 381, 394
MseI TTAA 2 cut(s) 279, 291
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 84
MwoI GCNNNNNNNGC 1 cut(s) 192
NdeII GATC 4 cut(s) 47, 142, 206, 217
NlaIII CATG 1 cut(s) 464
NlaIV GGNNCC 1 cut(s) 369
NmuCI GTSAC 2 cut(s) 247, 418
NspI RCATGY 1 cut(s) 464
PasI CCCWGGG 1 cut(s) 83
PciI ACATGT 1 cut(s) 460
PkrI GCNGC 2 cut(s) 28, 241
PscI ACATGT 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 2 cut(s) 47, 206
RsaI GTAC 1 cut(s) 453
RsaNI GTAC 1 cut(s) 452
SaqAI TTAA 2 cut(s) 279, 291
SatI GCNGC 2 cut(s) 27, 240
Sau3AI GATC 4 cut(s) 47, 142, 206, 217
ScrFI CCNGG 1 cut(s) 84
SetI ASST 6 cut(s) 76, 103, 360, 439, 503, 508
SfaNI GCATC 2 cut(s) 479, 514
SfcI CTRYAG 2 cut(s) 75, 189
SmlI CTYRAG 2 cut(s) 348, 372
SmoI CTYRAG 2 cut(s) 348, 372
Sse9I AATT 5 cut(s) 35, 223, 275, 492, 547
SsiI CCGC 1 cut(s) 27
SspI AATATT 1 cut(s) 333
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 1 cut(s) 360
TaqII GACCGA 1 cut(s) 124
TasI AATT 5 cut(s) 35, 223, 275, 492, 547
TatI WGTACW 1 cut(s) 451
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 2 cut(s) 279, 291
Tru9I TTAA 2 cut(s) 279, 291
TscAI CASTG 1 cut(s) 28
TseFI GTSAC 2 cut(s) 247, 418
TseI GCWGC 1 cut(s) 239
Tsp45I GTSAC 2 cut(s) 247, 418
TspDTI ATGAA 4 cut(s) 17, 111, 462, 485
TspRI CASTG 1 cut(s) 28
XapI RAATTY 3 cut(s) 35, 275, 492
XceI RCATGY 1 cut(s) 464
XspI CTAG 1 cut(s) 236
ZraI GACGTC 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.