Rh1DG061500

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001233
Physical Location & Seq
Reverse (-)
10823927 .. 10829293
5367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG061500.1

Sequence Viewer

Length: 1560 bp
ATGTCTATGTGGACCAGGTTGCAGCCTGCCTATGCTGCAAACATGGGGAATGAGGCTCTAAACAAGCGGGTAGGTACTGAGGTAATTGTGCTTCCCTTGTGGATGCTCAGTATTTTCAAGAATGAATTGGCACAGGGATTGAACCTGTATGAGATTCTGGCTGAGGTTGAAAATCGTGAGATATCTGTTGATCACTTGCTTACTATTCCAGAACAAGATGAGTGGGTGTACAGTGATGGCAAATCTACAACCTGTGTTGCTTTCATTCTTGCAATGTATAAAGAAGCTGGGATTTTTGGTCCTATTTCTAACTCCATTCAAGTTACTGAATTCACTGGGATACTAACAACAAACTCAGCAGGCAATGGTGGTCCTTTTGATGGAAGTGTAGCACCATGGATGCTAACAGTTGCAGCAAGTACAATAGATCGACGGATCATTGACAAGGTGATTCTTGGAAATGGAAAAACATTAGTCGCGGCGTTGGTAAACTCTTTCACATTAAATGGACCAAGTTTTCTATTGATTTATGGAAATGGCGCTTCAAGTCGATGCTCAAACTTTAGTACTAGGACTTGTATAGATGGCTTCTTAGATAGTGATTTAGTTAAGGGAAAGGTTTTGTTATGTGATGAGTCAGATGGAGCTAATGGGGCCTTTCTTTCTGGAGCAGTAGGTTCAATTATGAAAGTTGATTGGGATGACTATTCTCTAGTTCCCCCACTTCCTGCAACGAGTTTGAGCCTCAATAACCATAATGTGATCAAGTCCTACATGAACTCCATGAAAGATCCTCAAGTAAACATACTCAGAAGTGAAGTCATAAAAGATGTTTCTGCACTCATTGTGGCTTCTTTCTCTTCACGTGGACCAAATTTACTTTTACCTGAAATTATCAAGCCTGATGTAAGCGCCCCAGGGGTGGATATCTTAGCTGCATATATTCCTATTGCTTCTATCTCATGTAGCCAAGATGACAAGCGGCACCTGAATTATAGTATTCTATCTAGAACCTCCATGTCTTGTCTGCACGCGGCTGGTGCAGCTGCATATGTGAAAGAAGTCCACCCTGATTGGTCTCCAGCAGCCATCAAATCATCTCCTATGACTACTGCTTGGGCCATTAACATCACTAACAAAAATAATGCCCCTGGTGAATTTGCTAATGGATCTGGACATATAAATCCTATTCAAGCTATAAACCTAGGGCTAGTGTATGAAGCATCTAAGGAAGATTACAAAAAGTTGCTTTGCTTGGTCATAGGCAAAGAAAAAGTTAGACTTATATCAAGAGATAACAACACCACTTGCCCTACTGGCTCTGACACAGGATCTCCATGGGATCACAATTATCCTTCACTGGCAGTTGTTGTCACACCAATGGAACCTTTTTCGATCAAATTCCAGAGGATTGTAAAAAATGTTGGCCTTCCAAACTCCACTTATAAGGAAAATATTTTGGCAACCTCCACTTACAAGGCCAACTTCACTCAATTCAACAAAGTTTTGCCTCAAGTTCTTTCCTTCGAGTCCTTGAATGAGGAGATTGAAGACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

519

Amino Acids

56.31

Weight (kDa)

5.32

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 142 - 380 3.6e-15 Subtilase family
fn3_6 PF17766 448 - 515 2.3e-06 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1446
AccB1I GGYRCC 1 cut(s) 984
AccII CGCG 2 cut(s) 479, 1034
AciI CCGC 4 cut(s) 67, 479, 982, 1034
AclWI GGATC 5 cut(s) 443, 785, 1177, 1339, 1350
AcsI RAATTY 4 cut(s) 329, 874, 1157, 1400
AcvI CACGTG 1 cut(s) 866
AfaI GTAC 4 cut(s) 76, 230, 421, 568
AfiI CCNNNNNNNGG 2 cut(s) 380, 922
AjnI CCWGG 3 cut(s) 14, 916, 1150
AluBI AGCT 5 cut(s) 287, 647, 935, 1046, 1196
AluI AGCT 5 cut(s) 287, 647, 935, 1046, 1196
Alw26I GTCTC 1 cut(s) 1083
AlwI GGATC 5 cut(s) 443, 785, 1177, 1339, 1350
AoxI GGCC 4 cut(s) 654, 1119, 1426, 1479
ApeKI GCWGC 7 cut(s) 22, 35, 413, 935, 1043, 1046, 1085
ApoI RAATTY 4 cut(s) 329, 874, 1157, 1400
AspA2I CCTAGG 1 cut(s) 1204
AspLEI GCGC 2 cut(s) 542, 914
AspS9I GGNCC 7 cut(s) 12, 299, 371, 509, 654, 869, 1119
AsuHPI GGTGA 2 cut(s) 460, 1166
AvaII GGWCC 5 cut(s) 12, 299, 371, 509, 869
AvrII CCTAGG 1 cut(s) 1204
BaeI ACNNNNGTAYC 2 cut(s) 66, 99
BanI GGYRCC 1 cut(s) 984
BarI GAAGNNNNNNTAC 2 cut(s) 75, 107
BbrPI CACGTG 1 cut(s) 866
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 7 cut(s) 22, 34, 425, 922, 1033, 1055, 1097
BccI CCATC 5 cut(s) 230, 374, 578, 635, 1097
BciT130I CCWGG 3 cut(s) 16, 918, 1152
BciVI GTATCC 1 cut(s) 333
BclI TGATCA 2 cut(s) 190, 762
BcoDI GTCTC 1 cut(s) 1083
BfaI CTAG 5 cut(s) 570, 713, 1008, 1205, 1211
BfoI RGCGCY 2 cut(s) 543, 915
BfuI GTATCC 1 cut(s) 333
BglI GCCNNNNNGGC 1 cut(s) 1317
BlnI CCTAGG 1 cut(s) 1204
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 3 cut(s) 16, 918, 1152
Bme18I GGWCC 5 cut(s) 12, 299, 371, 509, 869
BmgT120I GGNCC 7 cut(s) 12, 299, 371, 509, 654, 869, 1119
BmiI GGNNCC 3 cut(s) 655, 986, 1386
BmrFI CCNGG 3 cut(s) 16, 918, 1152
BmrI ACTGGG 1 cut(s) 345
BmsI GCATC 4 cut(s) 93, 390, 542, 1232
BmuI ACTGGG 1 cut(s) 345
BpmI CTGGAG 2 cut(s) 687, 1065
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 2 cut(s) 780, 1497
BsaAI YACGTR 1 cut(s) 866
BsaI GGTCTC 1 cut(s) 1083
BsaJI CCNNGG 6 cut(s) 395, 916, 917, 1150, 1204, 1337
BsaXI ACNNNNNCTCC 4 cut(s) 764, 794, 1318, 1348
Bsc4I CCNNNNNNNGG 2 cut(s) 380, 922
Bse1I ACTGG 3 cut(s) 340, 1321, 1365
Bse3DI GCAATG 2 cut(s) 279, 370
BseBI CCWGG 3 cut(s) 16, 918, 1152
BseDI CCNNGG 6 cut(s) 395, 916, 917, 1150, 1204, 1337
BseGI GGATG 3 cut(s) 108, 405, 706
BseLI CCNNNNNNNGG 2 cut(s) 380, 922
BseMI GCAATG 2 cut(s) 279, 370
BseMII CTCAG 5 cut(s) 69, 121, 153, 369, 823
BseNI ACTGG 3 cut(s) 340, 1321, 1365
BseRI GAGGAG 1 cut(s) 1556
BseXI GCAGC 7 cut(s) 22, 34, 425, 922, 1033, 1055, 1097
BseYI CCCAGC 1 cut(s) 287
BsgI GTGCAG 3 cut(s) 822, 1013, 1062
Bsh1236I CGCG 2 cut(s) 479, 1034
BshFI GGCC 4 cut(s) 656, 1121, 1428, 1481
BshNI GGYRCC 1 cut(s) 984
BslI CCNNNNNNNGG 2 cut(s) 380, 922
BsmAI GTCTC 1 cut(s) 1083
BsnI GGCC 4 cut(s) 656, 1121, 1428, 1481
Bso31I GGTCTC 1 cut(s) 1083
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 9 cut(s) 190, 427, 435, 762, 790, 1169, 1331, 1342, 1395
Bsp19I CCATGG 2 cut(s) 395, 1337
BspACI CCGC 4 cut(s) 67, 479, 982, 1034
BspANI GGCC 4 cut(s) 656, 1121, 1428, 1481
BspCNI CTCAG 5 cut(s) 70, 120, 154, 368, 822
BspFNI CGCG 2 cut(s) 479, 1034
BspLI GGNNCC 3 cut(s) 655, 986, 1386
BspPI GGATC 5 cut(s) 443, 785, 1177, 1339, 1350
BspT107I GGYRCC 1 cut(s) 984
BspTNI GGTCTC 1 cut(s) 1083
BsrDI GCAATG 2 cut(s) 279, 370
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 3 cut(s) 340, 1321, 1365
BssECI CCNNGG 6 cut(s) 395, 916, 917, 1150, 1204, 1337
BssMI GATC 9 cut(s) 190, 427, 435, 762, 790, 1169, 1331, 1342, 1395
BssT1I CCWWGG 3 cut(s) 395, 1204, 1337
Bst2UI CCWGG 3 cut(s) 16, 918, 1152
Bst4CI ACNGT 2 cut(s) 233, 409
Bst6I CTCTTC 1 cut(s) 865
BstAUI TGTACA 1 cut(s) 228
BstBAI YACGTR 1 cut(s) 866
BstC8I GCNNGC 3 cut(s) 27, 361, 1032
BstDEI CTNAG 8 cut(s) 78, 107, 162, 355, 592, 809, 931, 1227
BstDSI CCRYGG 2 cut(s) 395, 1337
BstF5I GGATG 3 cut(s) 108, 405, 706
BstFNI CGCG 2 cut(s) 479, 1034
BstH2I RGCGCY 2 cut(s) 543, 915
BstHHI GCGC 2 cut(s) 542, 914
BstKTI GATC 9 cut(s) 193, 430, 438, 765, 793, 1172, 1334, 1345, 1398
BstMAI GTCTC 1 cut(s) 1083
BstMBI GATC 9 cut(s) 190, 427, 435, 762, 790, 1169, 1331, 1342, 1395
BstMWI GCNNNNNNNGC 5 cut(s) 35, 653, 1040, 1043, 1317
BstNI CCWGG 3 cut(s) 16, 918, 1152
BstSCI CCNGG 3 cut(s) 14, 916, 1150
BstUI CGCG 2 cut(s) 479, 1034
BstV1I GCAGC 7 cut(s) 22, 34, 425, 922, 1033, 1055, 1097
BstX2I RGATCY 3 cut(s) 790, 1169, 1331
BstYI RGATCY 3 cut(s) 790, 1169, 1331
BsuI GTATCC 1 cut(s) 333
BsuRI GGCC 4 cut(s) 656, 1121, 1428, 1481
BtgI CCRYGG 2 cut(s) 395, 1337
BtsCI GGATG 3 cut(s) 108, 405, 706
BtsIMutI CAGTG 3 cut(s) 238, 333, 1358
Cac8I GCNNGC 3 cut(s) 27, 361, 1032
CfoI GCGC 2 cut(s) 542, 914
Cfr13I GGNCC 7 cut(s) 12, 299, 371, 509, 654, 869, 1119
CsiI ACCWGGT 1 cut(s) 14
Csp6I GTAC 4 cut(s) 75, 229, 420, 567
CspCI CAANNNNNGTGG 4 cut(s) 203, 238, 1055, 1090
CviAII CATG 7 cut(s) 43, 396, 775, 784, 963, 1018, 1338
CviQI GTAC 4 cut(s) 75, 229, 420, 567
DdeI CTNAG 8 cut(s) 78, 107, 162, 355, 592, 809, 931, 1227
DpnI GATC 9 cut(s) 192, 429, 437, 764, 792, 1171, 1333, 1344, 1397
DpnII GATC 9 cut(s) 190, 427, 435, 762, 790, 1169, 1331, 1342, 1395
Eam1104I CTCTTC 1 cut(s) 865
EarI CTCTTC 1 cut(s) 865
Eco130I CCWWGG 3 cut(s) 395, 1204, 1337
Eco31I GGTCTC 1 cut(s) 1083
Eco32I GATATC 2 cut(s) 183, 928
Eco47I GGWCC 5 cut(s) 12, 299, 371, 509, 869
Eco72I CACGTG 1 cut(s) 866
EcoO109I RGGNCCY 1 cut(s) 654
EcoRI GAATTC 1 cut(s) 329
EcoRII CCWGG 3 cut(s) 14, 916, 1150
EcoRV GATATC 2 cut(s) 183, 928
EcoT14I CCWWGG 3 cut(s) 395, 1204, 1337
ErhI CCWWGG 3 cut(s) 395, 1204, 1337
FaeI CATG 7 cut(s) 46, 399, 778, 787, 966, 1021, 1341
FalI AAGNNNNNCTT 4 cut(s) 1266, 1298, 1469, 1501
FatI CATG 7 cut(s) 42, 395, 774, 783, 962, 1017, 1337
FauI CCCGC 1 cut(s) 60
FauNDI CATATG 1 cut(s) 1051
FbaI TGATCA 2 cut(s) 190, 762
FokI GGATG 3 cut(s) 115, 412, 713
FspBI CTAG 5 cut(s) 570, 713, 1008, 1205, 1211
GlaI GCGC 2 cut(s) 541, 913
GsaI CCCAGC 1 cut(s) 291
GsuI CTGGAG 2 cut(s) 687, 1065
HaeII RGCGCY 2 cut(s) 543, 915
HaeIII GGCC 4 cut(s) 656, 1121, 1428, 1481
HhaI GCGC 2 cut(s) 542, 914
Hin1II CATG 7 cut(s) 46, 399, 778, 787, 966, 1021, 1341
Hin6I GCGC 2 cut(s) 540, 912
HinP1I GCGC 2 cut(s) 540, 912
HinfI GANTC 4 cut(s) 154, 451, 635, 1529
HphI GGTGA 2 cut(s) 460, 1166
Hpy166II GTNNAC 6 cut(s) 12, 229, 490, 802, 869, 1066
Hpy188I TCNGA 3 cut(s) 640, 812, 1324
Hpy188III TCNNGA 8 cut(s) 118, 176, 209, 666, 1008, 1173, 1290, 1405
Hpy8I GTNNAC 6 cut(s) 12, 229, 490, 802, 869, 1066
Hpy99I CGWCG 1 cut(s) 435
HpyAV CCTTC 3 cut(s) 1365, 1439, 1534
HpyCH4III ACNGT 2 cut(s) 233, 409
HpyCH4IV ACGT 1 cut(s) 865
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 653, 1040, 1043, 1317
HpyF3I CTNAG 8 cut(s) 78, 107, 162, 355, 592, 809, 931, 1227
HpySE526I ACGT 1 cut(s) 865
Hsp92II CATG 7 cut(s) 46, 399, 778, 787, 966, 1021, 1341
HspAI GCGC 2 cut(s) 540, 912
Ksp22I TGATCA 2 cut(s) 190, 762
Kzo9I GATC 9 cut(s) 190, 427, 435, 762, 790, 1169, 1331, 1342, 1395
LmnI GCTCC 2 cut(s) 644, 668
Lsp1109I GCAGC 7 cut(s) 22, 34, 425, 922, 1033, 1055, 1097
LweI GCATC 4 cut(s) 93, 390, 542, 1232
MabI ACCWGGT 1 cut(s) 14
MaeI CTAG 5 cut(s) 570, 713, 1008, 1205, 1211
MaeII ACGT 1 cut(s) 865
MaeIII GTNAC 2 cut(s) 322, 1372
MalI GATC 9 cut(s) 192, 429, 437, 764, 792, 1171, 1333, 1344, 1397
MboI GATC 9 cut(s) 190, 427, 435, 762, 790, 1169, 1331, 1342, 1395
MboII GAAGA 2 cut(s) 852, 1244
MflI RGATCY 3 cut(s) 790, 1169, 1331
MlyI GAGTC 2 cut(s) 644, 1538
MseI TTAA 3 cut(s) 503, 609, 1125
MslI CAYNNNNRTG 1 cut(s) 1379
MspA1I CMGCKG 1 cut(s) 1046
MspR9I CCNGG 3 cut(s) 16, 918, 1152
MvaI CCWGG 3 cut(s) 16, 918, 1152
MvnI CGCG 2 cut(s) 479, 1034
MwoI GCNNNNNNNGC 5 cut(s) 35, 653, 1040, 1043, 1317
NcoI CCATGG 2 cut(s) 395, 1337
NdeI CATATG 1 cut(s) 1051
NdeII GATC 9 cut(s) 190, 427, 435, 762, 790, 1169, 1331, 1342, 1395
NlaIII CATG 7 cut(s) 46, 399, 778, 787, 966, 1021, 1341
NlaIV GGNNCC 3 cut(s) 655, 986, 1386
NmuCI GTSAC 1 cut(s) 1372
PasI CCCWGGG 1 cut(s) 917
PfeI GAWTC 2 cut(s) 154, 451
PleI GAGTC 2 cut(s) 643, 1537
PmaCI CACGTG 1 cut(s) 866
PmlI CACGTG 1 cut(s) 866
PpsI GAGTC 2 cut(s) 643, 1537
Ppu21I YACGTR 1 cut(s) 866
PsiI TTATAA 1 cut(s) 1446
Psp6I CCWGG 3 cut(s) 14, 916, 1150
PspCI CACGTG 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 287
PspGI CCWGG 3 cut(s) 14, 916, 1150
PspN4I GGNNCC 3 cut(s) 655, 986, 1386
PspPI GGNCC 7 cut(s) 12, 299, 371, 509, 654, 869, 1119
PsuI RGATCY 3 cut(s) 790, 1169, 1331
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 4 cut(s) 76, 230, 421, 568
RsaNI GTAC 4 cut(s) 75, 229, 420, 567
RseI CAYNNNNRTG 1 cut(s) 1379
SaqAI TTAA 3 cut(s) 503, 609, 1125
Sau3AI GATC 9 cut(s) 190, 427, 435, 762, 790, 1169, 1331, 1342, 1395
Sau96I GGNCC 7 cut(s) 12, 299, 371, 509, 654, 869, 1119
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 2 cut(s) 644, 1538
ScrFI CCNGG 3 cut(s) 16, 918, 1152
SexAI ACCWGGT 1 cut(s) 14
SfaNI GCATC 4 cut(s) 93, 390, 542, 1232
SinI GGWCC 5 cut(s) 12, 299, 371, 509, 869
SmiMI CAYNNNNRTG 1 cut(s) 1379
SmlI CTYRAG 2 cut(s) 795, 1512
SmoI CTYRAG 2 cut(s) 795, 1512
SsiI CCGC 4 cut(s) 67, 479, 982, 1034
SspI AATATT 1 cut(s) 1456
SspMI CTAG 5 cut(s) 570, 713, 1008, 1205, 1211
StyD4I CCNGG 3 cut(s) 14, 916, 1150
StyI CCWWGG 3 cut(s) 395, 1204, 1337
TaaI ACNGT 2 cut(s) 233, 409
TaiI ACGT 1 cut(s) 868
TaqI TCGA 4 cut(s) 430, 550, 1394, 1527
TatI WGTACW 3 cut(s) 228, 419, 566
TauI GCSGC 3 cut(s) 482, 985, 1037
TfiI GAWTC 2 cut(s) 154, 451
Tru1I TTAA 3 cut(s) 503, 609, 1125
Tru9I TTAA 3 cut(s) 503, 609, 1125
TscAI CASTG 3 cut(s) 238, 340, 1365
TseFI GTSAC 1 cut(s) 1372
TseI GCWGC 7 cut(s) 22, 35, 413, 935, 1043, 1046, 1085
Tsp45I GTSAC 1 cut(s) 1372
TspDTI ATGAA 6 cut(s) 138, 253, 701, 791, 800, 1233
TspGWI ACGGA 1 cut(s) 448
TspRI CASTG 3 cut(s) 238, 340, 1365
VpaK11BI GGWCC 5 cut(s) 12, 299, 371, 509, 869
XapI RAATTY 4 cut(s) 329, 874, 1157, 1400
XbaI TCTAGA 1 cut(s) 1007
XmaJI CCTAGG 1 cut(s) 1204
XspI CTAG 5 cut(s) 570, 713, 1008, 1205, 1211
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.