Rroxscaffold_4G00326660

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001233
Physical Location & Seq
Forward (+)
58817455 .. 58819949
2495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326660.1

Sequence Viewer

Length: 1422 bp
ATGAACTCCACGAAAGATCCACGAGTAAACATACTAAAGAGTGAAGCCGAAAGTGATGCTGCTGCGCCTGTTGTTGCTTCCTTCTCTTCACGTGGACCTAATCAAATTTTACCAGAAATTATCAAGCCAGATATAAGTGCCCCAGGGGTAGCTATATTGGCTGCTTATTCACCTATTTCTTCAATCACAGATAACCCTGAAGACAAGAGGCATGTAAATTACAGTATACTATCCGGAACTTCCATGTCATGCCCCCACGCCACTGGTGCAGCAGCGTATGTTAAAGAGTTCCACCCTGATTGGTCTCCAGCAGCCATCAAATCATCTCTTATGACTACAGCTTCTCCTATGAATTTTACCTACAATGATTATAATGCCGCTGCTGAGTTTGCTTGTGGATCTGGACATATCAATCCTGTCAAAGCTGTAGACCCGGTGCTTGTATATGAAGCTTCTAAGGATGACTACATAAAGTTGCTCTGCTTGGTCTTAAACCAGACCAGTGTTAGACTTATATCCGGAGATAACAGCACTTGCTCTACAGGCTCTGACAAAGGTTCTTTACTTGATCACAATTACCCATCGCTGGCAGCTGCTGTCACACCGAAGAAACCTTTTTCATTCAAATTTCACAGAAAAGTTAAGAATGTTGGCCTTGCAAATTCCACCTACAATGCCAAAATATTTGCCGGCAACTCTACTGGTGAAGCTGACATCAAAGTGGTGCCTGAAATCCTTTCCTTCAAGTCCTTGAATGAAGAGAAGACTTTTGATGTGACTGTTGTTGGAAAAGGTTTGTCAGAAGAAGTACAATCACATGTATCTGCATCACTGGTGTGGTCTGATGGGATTCACAGAGTTAGAACCATTGATAAAGATAGAAAGGTCCATATTGTGTACCTGGGATCACTTCCTGATGATGAGGTCTATTCATACATGTCTCATCACCTTGATATACTAGAAAGAGTTGTTGGGAGAAAATCCGCTGCAAAATTCTTCACAAGAAGTTACAAAAGAAGTTTCAATGGATTTGCAGCAGAGCTCACTGACCAGGAAAGAGAAAGGCTTTCTAGCATGAAGGAAGTAGTGTCTGTTTTTCCAAGTAGAAGTTTCCAGCTTCAAACAACAAGATCTTGGGACTTTATCGGGTTGTATGAGAAAATACATCAAAATGCAACTGTTGAGAGTGATGTAAGAGTGTTTGACAGTGGAATTTGGCCCGAATCTGAGAGCTCTAAAACTGAAGGTTGTGGTCCTGCCCCCAAGAAATTGAAAGGTGCTTGTGCAGGCAGTAAAAATTTCATTTGTAACAAGTTAACAACAAGATCATTGGAGCTTGGTTTTATGAGCCAGCAGATTCTGCAAGAGATGCAAATGGTCATGGAACCCATGTTGCCTCAACAGCAGCAGGGAACACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

473

Amino Acids

51.87

Weight (kDa)

6.87

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 20 - 133 2.8e-20 Subtilase family
fn3_6 PF17766 190 - 289 3.4e-19 Fibronectin type-III domain
Inhibitor_I9 PF05922 296 - 373 2.3e-14 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 724
AccI GTMKAC 2 cut(s) 226, 429
AccIII TCCGGA 2 cut(s) 233, 518
AciI CCGC 2 cut(s) 378, 984
AclWI GGATC 3 cut(s) 11, 406, 913
AcsI RAATTY 7 cut(s) 105, 352, 626, 661, 992, 1212, 1297
AcuI CTGAAG 2 cut(s) 219, 1263
AcvI CACGTG 1 cut(s) 92
AfaI GTAC 2 cut(s) 810, 899
AfiI CCNNNNNNNGG 1 cut(s) 586
AflIII ACRYGT 2 cut(s) 817, 936
AgsI TTSAA 7 cut(s) 183, 625, 745, 754, 1024, 1121, 1273
AjnI CCWGG 3 cut(s) 142, 900, 1050
AjuI GAANNNNNNNTTGG 2 cut(s) 954, 986
AleI CACNNNNGTG 1 cut(s) 835
Alw21I GWGCWC 2 cut(s) 1044, 1235
Alw26I GTCTC 2 cut(s) 309, 945
AlwI GGATC 3 cut(s) 11, 406, 913
AlwNI CAGNNNCTG 3 cut(s) 548, 596, 1360
Aor13HI TCCGGA 2 cut(s) 233, 518
AoxI GGCC 2 cut(s) 652, 1217
ApoI RAATTY 7 cut(s) 105, 352, 626, 661, 992, 1212, 1297
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 4 cut(s) 95, 886, 1218, 1253
AsuC2I CCSGG 1 cut(s) 434
AsuHPI GGTGA 3 cut(s) 162, 716, 938
AvaII GGWCC 3 cut(s) 95, 886, 1253
BaeGI GKGCMC 1 cut(s) 142
BanI GGYRCC 1 cut(s) 724
BanII GRGCYC 2 cut(s) 1044, 1235
BauI CACGAG 1 cut(s) 21
BbrPI CACGTG 1 cut(s) 92
BbsI GAAGAC 2 cut(s) 207, 770
Bbv12I GWGCWC 2 cut(s) 1044, 1235
BccI CCATC 3 cut(s) 323, 589, 839
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 3 cut(s) 144, 902, 1052
BclI TGATCA 1 cut(s) 568
BcnI CCSGG 1 cut(s) 434
BcoDI GTCTC 2 cut(s) 309, 945
BfaI CTAG 2 cut(s) 959, 1071
BfmI CTRYAG 3 cut(s) 336, 426, 540
BglII AGATCT 1 cut(s) 1130
Bme1390I CCNGG 4 cut(s) 144, 434, 902, 1052
Bme18I GGWCC 3 cut(s) 95, 886, 1253
BmgT120I GGNCC 4 cut(s) 95, 886, 1218, 1253
BmiI GGNNCC 2 cut(s) 726, 1386
BmrFI CCNGG 4 cut(s) 144, 434, 902, 1052
BmsI GCATC 3 cut(s) 46, 836, 1359
BpiI GAAGAC 2 cut(s) 207, 770
BpmI CTGGAG 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 434
BsaAI YACGTR 1 cut(s) 92
BsaI GGTCTC 1 cut(s) 309
BsaJI CCNNGG 3 cut(s) 142, 143, 901
BsaWI WCCGGW 2 cut(s) 233, 518
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 1 cut(s) 586
Bse118I RCCGGY 1 cut(s) 689
Bse1I ACTGG 4 cut(s) 268, 501, 706, 837
BseAI TCCGGA 2 cut(s) 233, 518
BseBI CCWGG 3 cut(s) 144, 902, 1052
BseDI CCNNGG 3 cut(s) 142, 143, 901
BseGI GGATG 1 cut(s) 466
BseLI CCNNNNNNNGG 1 cut(s) 586
BseMII CTCAG 2 cut(s) 375, 1218
BseNI ACTGG 4 cut(s) 268, 501, 706, 837
BseSI GKGCMC 1 cut(s) 142
BsgI GTGCAG 2 cut(s) 288, 1305
BshFI GGCC 2 cut(s) 654, 1219
BshNI GGYRCC 1 cut(s) 724
BsiHKAI GWGCWC 2 cut(s) 1044, 1235
BsiSI CCGG 4 cut(s) 234, 434, 519, 690
BslFI GGGAC 1 cut(s) 1151
BslI CCNNNNNNNGG 1 cut(s) 586
BsmAI GTCTC 2 cut(s) 309, 945
BsmFI GGGAC 1 cut(s) 1151
BsnI GGCC 2 cut(s) 654, 1219
Bso31I GGTCTC 1 cut(s) 309
Bsp1286I GDGCHC 3 cut(s) 142, 1044, 1235
Bsp13I TCCGGA 2 cut(s) 233, 518
Bsp143I GATC 6 cut(s) 16, 398, 568, 905, 1130, 1325
BspACI CCGC 2 cut(s) 378, 984
BspANI GGCC 2 cut(s) 654, 1219
BspCNI CTCAG 2 cut(s) 376, 1219
BspEI TCCGGA 2 cut(s) 233, 518
BspLI GGNNCC 2 cut(s) 726, 1386
BspPI GGATC 3 cut(s) 11, 406, 913
BspT107I GGYRCC 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 309
BsrFI RCCGGY 1 cut(s) 689
BsrI ACTGG 4 cut(s) 268, 501, 706, 837
BssAI RCCGGY 1 cut(s) 689
BssECI CCNNGG 3 cut(s) 142, 143, 901
BssMI GATC 6 cut(s) 16, 398, 568, 905, 1130, 1325
BssNAI GTATAC 1 cut(s) 227
BssSI CACGAG 1 cut(s) 21
Bst1107I GTATAC 1 cut(s) 227
Bst2BI CACGAG 1 cut(s) 21
Bst2UI CCWGG 3 cut(s) 144, 902, 1052
Bst4CI ACNGT 5 cut(s) 224, 781, 1180, 1208, 1419
Bst6I CTCTTC 2 cut(s) 91, 753
BstAPI GCANNNNNTGC 2 cut(s) 1360, 1369
BstBAI YACGTR 1 cut(s) 92
BstC8I GCNNGC 4 cut(s) 588, 691, 1288, 1352
BstDEI CTNAG 3 cut(s) 384, 456, 1227
BstF5I GGATG 1 cut(s) 466
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 6 cut(s) 19, 401, 571, 908, 1133, 1328
BstMAI GTCTC 2 cut(s) 309, 945
BstMBI GATC 6 cut(s) 16, 398, 568, 905, 1130, 1325
BstMWI GCNNNNNNNGC 7 cut(s) 158, 266, 389, 543, 1360, 1369, 1402
BstNI CCWGG 3 cut(s) 144, 902, 1052
BstNSI RCATGY 3 cut(s) 215, 821, 940
BstSCI CCNGG 4 cut(s) 142, 432, 900, 1050
BstSFI CTRYAG 3 cut(s) 336, 426, 540
BstSLI GKGCMC 1 cut(s) 142
BstV2I GAAGAC 2 cut(s) 207, 770
BstX2I RGATCY 3 cut(s) 16, 398, 1130
BstXI CCANNNNNNTGG 1 cut(s) 263
BstYI RGATCY 3 cut(s) 16, 398, 1130
BstZ17I GTATAC 1 cut(s) 227
BsuRI GGCC 2 cut(s) 654, 1219
BtgZI GCGATG 1 cut(s) 567
BtsCI GGATG 1 cut(s) 466
BtsIMutI CAGTG 6 cut(s) 261, 508, 830, 1044, 1213, 1415
Cac8I GCNNGC 4 cut(s) 588, 691, 1288, 1352
CaiI CAGNNNCTG 3 cut(s) 548, 596, 1360
CfoI GCGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 689
Cfr13I GGNCC 4 cut(s) 95, 886, 1218, 1253
Csp6I GTAC 2 cut(s) 809, 898
CspCI CAANNNNNGTGG 2 cut(s) 281, 316
CviAII CATG 8 cut(s) 212, 244, 249, 818, 937, 1075, 1381, 1390
CviQI GTAC 2 cut(s) 809, 898
DdeI CTNAG 3 cut(s) 384, 456, 1227
DpnI GATC 6 cut(s) 18, 400, 570, 907, 1132, 1327
DpnII GATC 6 cut(s) 16, 398, 568, 905, 1130, 1325
Eam1104I CTCTTC 2 cut(s) 91, 753
EarI CTCTTC 2 cut(s) 91, 753
Ecl136II GAGCTC 2 cut(s) 1042, 1233
Eco24I GRGCYC 2 cut(s) 1044, 1235
Eco31I GGTCTC 1 cut(s) 309
Eco47I GGWCC 3 cut(s) 95, 886, 1253
Eco53kI GAGCTC 2 cut(s) 1042, 1233
Eco57I CTGAAG 2 cut(s) 219, 1263
Eco72I CACGTG 1 cut(s) 92
EcoICRI GAGCTC 2 cut(s) 1042, 1233
EcoRII CCWGG 3 cut(s) 142, 900, 1050
EcoT38I GRGCYC 2 cut(s) 1044, 1235
FaeI CATG 8 cut(s) 215, 247, 252, 821, 940, 1078, 1384, 1393
FaqI GGGAC 1 cut(s) 1151
FatI CATG 8 cut(s) 211, 243, 248, 817, 936, 1074, 1380, 1389
FbaI TGATCA 1 cut(s) 568
FblI GTMKAC 2 cut(s) 226, 429
FokI GGATG 1 cut(s) 473
FriOI GRGCYC 2 cut(s) 1044, 1235
FspBI CTAG 2 cut(s) 959, 1071
GlaI GCGC 1 cut(s) 66
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 2 cut(s) 654, 1219
HapII CCGG 4 cut(s) 234, 434, 519, 690
HhaI GCGC 1 cut(s) 67
Hin1II CATG 8 cut(s) 215, 247, 252, 821, 940, 1078, 1384, 1393
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HincII GTYRAC 1 cut(s) 1317
HindII GTYRAC 1 cut(s) 1317
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 3 cut(s) 850, 1223, 1357
HpaI GTTAAC 1 cut(s) 1317
HpaII CCGG 4 cut(s) 234, 434, 519, 690
HphI GGTGA 3 cut(s) 162, 716, 938
Hpy166II GTNNAC 6 cut(s) 28, 95, 227, 430, 898, 1317
Hpy188I TCNGA 4 cut(s) 550, 802, 844, 1228
Hpy188III TCNNGA 4 cut(s) 234, 402, 519, 914
Hpy8I GTNNAC 6 cut(s) 28, 95, 227, 430, 898, 1317
HpyAV CCTTC 4 cut(s) 91, 751, 1072, 1238
HpyCH4III ACNGT 5 cut(s) 224, 781, 1180, 1208, 1419
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 9 cut(s) 269, 659, 827, 989, 1034, 1175, 1286, 1363, 1372
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 266, 389, 543, 1360, 1369, 1402
HpyF3I CTNAG 3 cut(s) 384, 456, 1227
HpySE526I ACGT 1 cut(s) 91
Hsp92II CATG 8 cut(s) 215, 247, 252, 821, 940, 1078, 1384, 1393
HspAI GCGC 1 cut(s) 65
Kpn2I TCCGGA 2 cut(s) 233, 518
KroI GCCGGC 1 cut(s) 689
KroNI GCCGGC 1 cut(s) 691
Ksp22I TGATCA 1 cut(s) 568
KspAI GTTAAC 1 cut(s) 1317
Kzo9I GATC 6 cut(s) 16, 398, 568, 905, 1130, 1325
LmnI GCTCC 1 cut(s) 1333
LweI GCATC 3 cut(s) 46, 836, 1359
MaeI CTAG 2 cut(s) 959, 1071
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 4 cut(s) 598, 775, 1007, 1307
MalI GATC 6 cut(s) 18, 400, 570, 907, 1132, 1327
MboI GATC 6 cut(s) 16, 398, 568, 905, 1130, 1325
MboII GAAGA 8 cut(s) 78, 171, 212, 619, 770, 775, 815, 988
MflI RGATCY 3 cut(s) 16, 398, 1130
MhlI GDGCHC 3 cut(s) 142, 1044, 1235
MmeI TCCRAC 1 cut(s) 766
MnlI CCTC 3 cut(s) 201, 916, 1407
MroI TCCGGA 2 cut(s) 233, 518
MroNI GCCGGC 1 cut(s) 689
MseI TTAA 4 cut(s) 282, 491, 642, 1316
MslI CAYNNNNRTG 3 cut(s) 719, 835, 1170
MspA1I CMGCKG 3 cut(s) 380, 593, 986
MspI CCGG 4 cut(s) 234, 434, 519, 690
MspR9I CCNGG 4 cut(s) 144, 434, 902, 1052
MvaI CCWGG 3 cut(s) 144, 902, 1052
MwoI GCNNNNNNNGC 7 cut(s) 158, 266, 389, 543, 1360, 1369, 1402
NaeI GCCGGC 1 cut(s) 691
NciI CCSGG 1 cut(s) 434
NdeII GATC 6 cut(s) 16, 398, 568, 905, 1130, 1325
NgoMIV GCCGGC 1 cut(s) 689
NlaIII CATG 8 cut(s) 215, 247, 252, 821, 940, 1078, 1384, 1393
NlaIV GGNNCC 2 cut(s) 726, 1386
NmuCI GTSAC 2 cut(s) 598, 775
NspI RCATGY 3 cut(s) 215, 821, 940
OliI CACNNNNGTG 1 cut(s) 835
PasI CCCWGGG 1 cut(s) 143
PciI ACATGT 2 cut(s) 817, 936
PdiI GCCGGC 1 cut(s) 691
PfeI GAWTC 3 cut(s) 850, 1223, 1357
PmaCI CACGTG 1 cut(s) 92
PmlI CACGTG 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 92
PscI ACATGT 2 cut(s) 817, 936
PsiI TTATAA 1 cut(s) 372
Psp124BI GAGCTC 2 cut(s) 1044, 1235
Psp6I CCWGG 3 cut(s) 142, 900, 1050
PspCI CACGTG 1 cut(s) 92
PspGI CCWGG 3 cut(s) 142, 900, 1050
PspN4I GGNNCC 2 cut(s) 726, 1386
PspPI GGNCC 4 cut(s) 95, 886, 1218, 1253
PstNI CAGNNNCTG 3 cut(s) 548, 596, 1360
PsuI RGATCY 3 cut(s) 16, 398, 1130
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 2 cut(s) 810, 899
RsaNI GTAC 2 cut(s) 809, 898
RseI CAYNNNNRTG 3 cut(s) 719, 835, 1170
SacI GAGCTC 2 cut(s) 1044, 1235
SaqAI TTAA 4 cut(s) 282, 491, 642, 1316
Sau3AI GATC 6 cut(s) 16, 398, 568, 905, 1130, 1325
Sau96I GGNCC 4 cut(s) 95, 886, 1218, 1253
ScrFI CCNGG 4 cut(s) 144, 434, 902, 1052
SduI GDGCHC 3 cut(s) 142, 1044, 1235
SfaNI GCATC 3 cut(s) 46, 836, 1359
SfcI CTRYAG 3 cut(s) 336, 426, 540
SinI GGWCC 3 cut(s) 95, 886, 1253
SmiMI CAYNNNNRTG 3 cut(s) 719, 835, 1170
SsiI CCGC 2 cut(s) 378, 984
SspI AATATT 1 cut(s) 684
SspMI CTAG 2 cut(s) 959, 1071
SstI GAGCTC 2 cut(s) 1044, 1235
StyD4I CCNGG 4 cut(s) 142, 432, 900, 1050
TaaI ACNGT 5 cut(s) 224, 781, 1180, 1208, 1419
TaiI ACGT 1 cut(s) 94
TatI WGTACW 1 cut(s) 808
TauI GCSGC 1 cut(s) 380
TfiI GAWTC 3 cut(s) 850, 1223, 1357
Tru1I TTAA 4 cut(s) 282, 491, 642, 1316
Tru9I TTAA 4 cut(s) 282, 491, 642, 1316
TscAI CASTG 6 cut(s) 268, 508, 837, 1051, 1213, 1422
TseFI GTSAC 2 cut(s) 598, 775
Tsp45I GTSAC 2 cut(s) 598, 775
TspDTI ATGAA 8 cut(s) 17, 365, 462, 609, 771, 921, 1091, 1291
TspRI CASTG 6 cut(s) 268, 508, 837, 1051, 1213, 1422
VpaK11BI GGWCC 3 cut(s) 95, 886, 1253
XapI RAATTY 7 cut(s) 105, 352, 626, 661, 992, 1212, 1297
XceI RCATGY 3 cut(s) 215, 821, 940
XmiI GTMKAC 2 cut(s) 226, 429
XspI CTAG 2 cut(s) 959, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.