pycom11g07680

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001233
Physical Location & Seq
Reverse (-)
5885784 .. 5886463
680 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g07680.2

Sequence Viewer

Length: 588 bp
ATGACCACTGCTTGGCCTATGAATGATACGTTGCCCACAAATGATACGTCCAACAATTTTAGTGGTGTATTTGCTTATGGATCCGGACATATCAATCCTGTAAAGGCTATAAACCCGGGCCTTGTGTACGAAACTTCTGAAGATGATCATATAAAGTTGCTATGCACAATCTATGATGAAGGAAAAGTTAGGCTCATAACAGGAGAAAATATCACATGCCCTAAAGATCCTGCGAAATGGTCTCCACGGGATCATAATTACCCTTCAATGGGAGCTAAAGTTGAGCCGACGAAACCTTTTACGGTTCAATTTCATAGAACAGTTAAAAATGTTGGCCTCGCGAACTCCACATATAGGGCTAAAATCTTATCAAACTCCAAACTTGATATCCAAGTGGTGCTTGAAGTTCTTTCGTTCAAGTCCTTGAATGAGCAGATGGATTTTGACGTGACTGTTGGTGGAAGCGATTTGCAAGATCGATCACAACTATCTGGGTCACTGGTCTGGTCTGATGGTATTCATAGCGTTAGAAGTCCAATCCTGGTTTATGCATATGTTCCAAATGAGCTCCAGAACATAGTTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

196

Amino Acids

21.6

Weight (kDa)

5.69

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 12
AccII CGCG 1 cut(s) 341
AccIII TCCGGA 1 cut(s) 83
AclWI GGATC 4 cut(s) 75, 88, 221, 258
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 4 cut(s) 12, 268, 269, 354
AgsI TTSAA 5 cut(s) 267, 308, 404, 418, 427
AjiI CACGTC 1 cut(s) 448
AjnI CCWGG 1 cut(s) 540
AluBI AGCT 2 cut(s) 275, 568
AluI AGCT 2 cut(s) 275, 568
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 4 cut(s) 75, 88, 221, 258
Ama87I CYCGRG 1 cut(s) 115
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 3 cut(s) 14, 118, 334
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AspS9I GGNCC 1 cut(s) 118
AsuC2I CCSGG 2 cut(s) 116, 117
AvaI CYCGRG 1 cut(s) 115
BamHI GGATCC 1 cut(s) 80
BanII GRGCYC 1 cut(s) 570
Bbv12I GWGCWC 1 cut(s) 570
BccI CCATC 2 cut(s) 430, 506
BciT130I CCWGG 1 cut(s) 542
BclI TGATCA 1 cut(s) 145
BcnI CCSGG 2 cut(s) 116, 117
BcoDI GTCTC 1 cut(s) 246
Bme1390I CCNGG 3 cut(s) 116, 117, 542
BmeT110I CYCGRG 1 cut(s) 115
BmgBI CACGTC 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 82
BmrFI CCNGG 3 cut(s) 116, 117, 542
BpmI CTGGAG 1 cut(s) 554
BpuMI CCSGG 2 cut(s) 116, 117
Bsa29I ATCGAT 1 cut(s) 478
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 2 cut(s) 115, 245
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 268, 269, 354
Bse1I ACTGG 1 cut(s) 504
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 1 cut(s) 542
BseCI ATCGAT 1 cut(s) 478
BseDI CCNNGG 2 cut(s) 115, 245
BseLI CCNNNNNNNGG 4 cut(s) 12, 268, 269, 354
BseNI ACTGG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 3 cut(s) 16, 120, 336
BshVI ATCGAT 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 570
BsiHKCI CYCGRG 1 cut(s) 115
BsiSI CCGG 2 cut(s) 84, 116
BslI CCNNNNNNNGG 4 cut(s) 12, 268, 269, 354
BsmAI GTCTC 1 cut(s) 246
BsnI GGCC 3 cut(s) 16, 120, 336
Bso31I GGTCTC 1 cut(s) 246
BsoBI CYCGRG 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 570
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 6 cut(s) 80, 145, 226, 250, 475, 479
Bsp68I TCGCGA 1 cut(s) 341
BspANI GGCC 3 cut(s) 16, 120, 336
BspDI ATCGAT 1 cut(s) 478
BspEI TCCGGA 1 cut(s) 83
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 1 cut(s) 82
BspPI GGATC 4 cut(s) 75, 88, 221, 258
BspTNI GGTCTC 1 cut(s) 246
BsrI ACTGG 1 cut(s) 504
BssECI CCNNGG 2 cut(s) 115, 245
BssMI GATC 6 cut(s) 80, 145, 226, 250, 475, 479
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 3 cut(s) 304, 322, 454
BstDSI CCRYGG 1 cut(s) 245
BstFNI CGCG 1 cut(s) 341
BstKTI GATC 6 cut(s) 83, 148, 229, 253, 478, 482
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 6 cut(s) 80, 145, 226, 250, 475, 479
BstNI CCWGG 1 cut(s) 542
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 3 cut(s) 114, 115, 540
BstUI CGCG 1 cut(s) 341
BstX2I RGATCY 2 cut(s) 80, 226
BstYI RGATCY 2 cut(s) 80, 226
Bsu15I ATCGAT 1 cut(s) 478
BsuRI GGCC 3 cut(s) 16, 120, 336
BsuTUI ATCGAT 1 cut(s) 478
BtgI CCRYGG 1 cut(s) 245
BtrI CACGTC 1 cut(s) 448
BtsI GCAGTG 1 cut(s) 6
BtsIMutI CAGTG 2 cut(s) 6, 497
BtuMI TCGCGA 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 118
Cfr9I CCCGGG 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 478
Csp6I GTAC 1 cut(s) 127
CspCI CAANNNNNGTGG 2 cut(s) 43, 78
CviAII CATG 2 cut(s) 216, 585
CviJI RGCY 9 cut(s) 16, 107, 120, 193, 275, 286, 336, 359, 568
CviKI_1 RGCY 9 cut(s) 16, 107, 120, 193, 275, 286, 336, 359, 568
CviQI GTAC 1 cut(s) 127
DpnI GATC 6 cut(s) 82, 147, 228, 252, 477, 481
DpnII GATC 6 cut(s) 80, 145, 226, 250, 475, 479
Ecl136II GAGCTC 1 cut(s) 568
Eco24I GRGCYC 1 cut(s) 570
Eco31I GGTCTC 1 cut(s) 246
Eco32I GATATC 1 cut(s) 388
Eco53kI GAGCTC 1 cut(s) 568
Eco57I CTGAAG 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 115
EcoICRI GAGCTC 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 540
EcoRV GATATC 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 553
EcoT38I GRGCYC 1 cut(s) 570
FaeI CATG 2 cut(s) 219, 588
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 2 cut(s) 215, 584
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 1 cut(s) 145
FriOI GRGCYC 1 cut(s) 570
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 3 cut(s) 16, 120, 336
HapII CCGG 2 cut(s) 84, 116
Hin1II CATG 2 cut(s) 219, 588
HpaII CCGG 2 cut(s) 84, 116
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 2 cut(s) 139, 511
Hpy188III TCNNGA 3 cut(s) 84, 340, 571
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 2 cut(s) 173, 273
HpyCH4III ACNGT 3 cut(s) 304, 322, 454
HpyCH4IV ACGT 3 cut(s) 29, 47, 447
HpyCH4V TGCA 3 cut(s) 165, 472, 551
HpySE526I ACGT 3 cut(s) 29, 47, 447
Hsp92II CATG 2 cut(s) 219, 588
Kpn2I TCCGGA 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 6 cut(s) 80, 145, 226, 250, 475, 479
LmnI GCTCC 2 cut(s) 272, 573
MaeII ACGT 3 cut(s) 29, 47, 447
MaeIII GTNAC 3 cut(s) 448, 495, 580
MalI GATC 6 cut(s) 82, 147, 228, 252, 477, 481
MboI GATC 6 cut(s) 80, 145, 226, 250, 475, 479
MboII GAAGA 1 cut(s) 152
MflI RGATCY 2 cut(s) 80, 226
MhlI GDGCHC 1 cut(s) 570
MluCI AATT 3 cut(s) 55, 256, 308
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 1 cut(s) 347
Mph1103I ATGCAT 1 cut(s) 553
MroI TCCGGA 1 cut(s) 83
MseI TTAA 1 cut(s) 324
MspI CCGG 2 cut(s) 84, 116
MspR9I CCNGG 3 cut(s) 116, 117, 542
MvaI CCWGG 1 cut(s) 542
MvnI CGCG 1 cut(s) 341
NciI CCSGG 2 cut(s) 116, 117
NdeI CATATG 1 cut(s) 553
NdeII GATC 6 cut(s) 80, 145, 226, 250, 475, 479
NlaIII CATG 2 cut(s) 219, 588
NlaIV GGNNCC 1 cut(s) 82
NmuCI GTSAC 2 cut(s) 448, 495
NruI TCGCGA 1 cut(s) 341
NsiI ATGCAT 1 cut(s) 553
NspI RCATGY 1 cut(s) 219
PflMI CCANNNNNTGG 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 540
PspGI CCWGG 1 cut(s) 540
PspN4I GGNNCC 1 cut(s) 82
PspPI GGNCC 1 cut(s) 118
PsuI RGATCY 2 cut(s) 80, 226
RruI TCGCGA 1 cut(s) 341
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SacI GAGCTC 1 cut(s) 570
SaqAI TTAA 1 cut(s) 324
Sau3AI GATC 6 cut(s) 80, 145, 226, 250, 475, 479
Sau96I GGNCC 1 cut(s) 118
ScrFI CCNGG 3 cut(s) 116, 117, 542
SduI GDGCHC 1 cut(s) 570
SetI ASST 6 cut(s) 32, 50, 277, 298, 450, 570
SmaI CCCGGG 1 cut(s) 117
Sse9I AATT 3 cut(s) 55, 256, 308
SstI GAGCTC 1 cut(s) 570
StyD4I CCNGG 3 cut(s) 114, 115, 540
TaaI ACNGT 3 cut(s) 304, 322, 454
TaiI ACGT 3 cut(s) 32, 50, 450
TaqI TCGA 1 cut(s) 478
TasI AATT 3 cut(s) 55, 256, 308
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 2 cut(s) 13, 504
TseFI GTSAC 2 cut(s) 448, 495
Tsp45I GTSAC 2 cut(s) 448, 495
TspDTI ATGAA 4 cut(s) 35, 192, 302, 509
TspMI CCCGGG 1 cut(s) 115
TspRI CASTG 2 cut(s) 13, 504
Van91I CCANNNNNTGG 1 cut(s) 12
XceI RCATGY 1 cut(s) 219
XmaI CCCGGG 1 cut(s) 115
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.