MD03G1083700.v1.1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001233
Physical Location & Seq
Reverse (-)
6781601 .. 6782785
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1083700.v1.1.491

Sequence Viewer

Length: 879 bp
ATGAACTCCACCAGAGATCCCCGAGCAAACATATTAAAAAGTGAAGTCATAAAAGATCTTGTTGCACCTGTTGTCGCCTCCTTCTCTACACATGGACCGAATCTAATTTTGCCCGATATTATCAAGCCAGATTTAAGTGCCCCAGGAGTAGAAATTTTGGCTGCATATTCGCCTAATGCTTCTATCTCAATTCCGGAAGACATAAGGCGCGTTAAATACACCATACTATCTGGAACTTCCATGGCTTGCCCTCATGCCACTGGTGTAGCTGCATACGTTAAAACCTTCCACCCTGATTGGTCTCCCGTAGCCATCAAATCCTCTCTTATGACTACCGCTTCGCTCATCAATGATACAAACAGCAGCATTTTGGATGGTGAATTTGCCTTTGGTTCTGGACATATCAATCCTGTAAAAGCAATAGACCCCGGGCTTGTGTATGAAGCATCTAAAGAAGATTACATAAAGTTGCTATGCACTAGATATGATGAAAGCACGGTTAGACTTATATCAGGAGATAACAGCAGTTGCCCTACAGGTCCTGAAAAAGGGTCACCGGTGGAACTTAATTACCCTTCGATGGGAGCTAAAGTAGCAGCAATGGAACCTTTTACGGTTAAATTCCATAGACGAGTTAAAAATGTAGGCCTTGCAAACTCAACTTACAATGCCAAAATCTCACCAAATGCCAAAGTAGATATCAAAGTGGCGCCGGAAGTTCTTTCCTTCAAGTCCCTGAATGAGGATCAGACCTTTGATGTCACCATTGTTGGGAGCAGTTCACAATTCGAATCACGAGTGTCTGCTGCCCTTGTTTGGTCTGATGGAACTCATACCGTTAGAAGTCCGATTGTTGTATACACGGTACCTAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

293

Amino Acids

31.38

Weight (kDa)

6.6

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 24 - 131 3.8e-20 Subtilase family
fn3_6 PF17766 188 - 286 8.3e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 865
AccB1I GGYRCC 2 cut(s) 709, 865
AccI GTMKAC 1 cut(s) 858
AccII CGCG 1 cut(s) 210
AccIII TCCGGA 1 cut(s) 193
AciI CCGC 1 cut(s) 336
AclWI GGATC 2 cut(s) 11, 753
AcsI RAATTY 3 cut(s) 153, 380, 620
AcyI GRCGYC 1 cut(s) 710
AfaI GTAC 1 cut(s) 867
AfiI CCNNNNNNNGG 6 cut(s) 548, 580, 581, 742, 771, 816
AgeI ACCGGT 1 cut(s) 556
AgsI TTSAA 1 cut(s) 730
AjnI CCWGG 1 cut(s) 142
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AluBI AGCT 2 cut(s) 269, 587
AluI AGCT 2 cut(s) 269, 587
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 2 cut(s) 11, 753
AlwNI CAGNNNCTG 1 cut(s) 542
Ama87I CYCGRG 2 cut(s) 21, 428
Aor13HI TCCGGA 1 cut(s) 193
AoxI GGCC 1 cut(s) 646
ApeKI GCWGC 5 cut(s) 161, 269, 363, 596, 806
ApoI RAATTY 3 cut(s) 153, 380, 620
ArsI GACNNNNNNTTYG 2 cut(s) 322, 354
AsiGI ACCGGT 1 cut(s) 556
Asp718I GGTACC 1 cut(s) 865
AspLEI GCGC 2 cut(s) 210, 712
AspS9I GGNCC 2 cut(s) 95, 539
AsuC2I CCSGG 2 cut(s) 429, 430
AsuHPI GGTGA 4 cut(s) 389, 546, 672, 754
AsuII TTCGAA 1 cut(s) 789
AvaI CYCGRG 2 cut(s) 21, 428
AvaII GGWCC 2 cut(s) 95, 539
BaeGI GKGCMC 1 cut(s) 142
BanI GGYRCC 2 cut(s) 709, 865
BauI CACGAG 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 204
BbvI GCAGC 5 cut(s) 148, 256, 375, 608, 793
BccI CCATC 4 cut(s) 320, 368, 574, 818
BciT130I CCWGG 1 cut(s) 144
BcnI CCSGG 2 cut(s) 429, 430
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 2 cut(s) 480, 870
BfmI CTRYAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 713
BglII AGATCT 1 cut(s) 55
BisI GCNGC 5 cut(s) 162, 270, 364, 597, 807
BlsI GCNGC 5 cut(s) 163, 271, 365, 598, 808
Bme1390I CCNGG 3 cut(s) 144, 429, 430
Bme18I GGWCC 2 cut(s) 95, 539
BmeT110I CYCGRG 2 cut(s) 21, 428
BmgT120I GGNCC 2 cut(s) 95, 539
BmiI GGNNCC 3 cut(s) 606, 711, 867
BmrFI CCNGG 3 cut(s) 144, 429, 430
BmsI GCATC 1 cut(s) 455
BpiI GAAGAC 1 cut(s) 204
Bpu14I TTCGAA 1 cut(s) 789
BpuMI CCSGG 2 cut(s) 429, 430
BsaHI GRCGYC 1 cut(s) 710
BsaI GGTCTC 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 142, 240, 427, 428
BsaWI WCCGGW 2 cut(s) 193, 556
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 6 cut(s) 548, 580, 581, 742, 771, 816
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 606
BseAI TCCGGA 1 cut(s) 193
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 4 cut(s) 142, 240, 427, 428
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 6 cut(s) 548, 580, 581, 742, 771, 816
BseMI GCAATG 1 cut(s) 606
BseNI ACTGG 1 cut(s) 265
BseSI GKGCMC 1 cut(s) 142
BseXI GCAGC 5 cut(s) 148, 256, 375, 608, 793
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 1 cut(s) 648
BshNI GGYRCC 2 cut(s) 709, 865
BshTI ACCGGT 1 cut(s) 556
BsiHKCI CYCGRG 2 cut(s) 21, 428
BsiSI CCGG 4 cut(s) 194, 429, 557, 713
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 6 cut(s) 548, 580, 581, 742, 771, 816
BsmAI GTCTC 1 cut(s) 306
BsmFI GGGAC 1 cut(s) 718
BsnI GGCC 1 cut(s) 648
Bso31I GGTCTC 1 cut(s) 306
BsoBI CYCGRG 2 cut(s) 21, 428
Bsp119I TTCGAA 1 cut(s) 789
Bsp1286I GDGCHC 1 cut(s) 142
Bsp13I TCCGGA 1 cut(s) 193
Bsp143I GATC 3 cut(s) 16, 55, 745
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 1 cut(s) 336
BspANI GGCC 1 cut(s) 648
BspEI TCCGGA 1 cut(s) 193
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 3 cut(s) 606, 711, 867
BspPI GGATC 2 cut(s) 11, 753
BspT104I TTCGAA 1 cut(s) 789
BspT107I GGYRCC 2 cut(s) 709, 865
BspTNI GGTCTC 1 cut(s) 306
BsrDI GCAATG 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 556
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 4 cut(s) 142, 240, 427, 428
BssMI GATC 3 cut(s) 16, 55, 745
BssNAI GTATAC 1 cut(s) 859
BssNI GRCGYC 1 cut(s) 710
BssSI CACGAG 1 cut(s) 795
BssT1I CCWWGG 1 cut(s) 240
Bst1107I GTATAC 1 cut(s) 859
Bst2BI CACGAG 1 cut(s) 795
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 4 cut(s) 499, 616, 838, 865
BstACI GRCGYC 1 cut(s) 710
BstBI TTCGAA 1 cut(s) 789
BstC8I GCNNGC 1 cut(s) 247
BstDSI CCRYGG 1 cut(s) 240
BstEII GGTNACC 1 cut(s) 552
BstENI CCTNNNNNAGG 2 cut(s) 546, 740
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 1 cut(s) 210
BstH2I RGCGCY 1 cut(s) 713
BstHHI GCGC 2 cut(s) 210, 712
BstKTI GATC 3 cut(s) 19, 58, 748
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 3 cut(s) 16, 55, 745
BstMWI GCNNNNNNNGC 1 cut(s) 593
BstNI CCWGG 1 cut(s) 144
BstPI GGTNACC 1 cut(s) 552
BstSCI CCNGG 3 cut(s) 142, 427, 428
BstSFI CTRYAG 1 cut(s) 534
BstSLI GKGCMC 1 cut(s) 142
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 5 cut(s) 148, 256, 375, 608, 793
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 2 cut(s) 16, 55
BstYI RGATCY 2 cut(s) 16, 55
BstZ17I GTATAC 1 cut(s) 859
BsuRI GGCC 1 cut(s) 648
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 247
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 2 cut(s) 210, 712
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 2 cut(s) 95, 539
Cfr9I CCCGGG 1 cut(s) 428
Csp6I GTAC 1 cut(s) 866
CspAI ACCGGT 1 cut(s) 556
CspCI CAANNNNNGTGG 2 cut(s) 278, 313
CviAII CATG 4 cut(s) 92, 241, 254, 876
CviJI RGCY 8 cut(s) 127, 161, 245, 269, 311, 433, 587, 648
CviKI_1 RGCY 8 cut(s) 127, 161, 245, 269, 311, 433, 587, 648
CviQI GTAC 1 cut(s) 866
DinI GGCGCC 1 cut(s) 711
DpnI GATC 3 cut(s) 18, 57, 747
DpnII GATC 3 cut(s) 16, 55, 745
Eco130I CCWWGG 1 cut(s) 240
Eco147I AGGCCT 1 cut(s) 648
Eco31I GGTCTC 1 cut(s) 306
Eco32I GATATC 1 cut(s) 700
Eco47I GGWCC 2 cut(s) 95, 539
Eco88I CYCGRG 2 cut(s) 21, 428
Eco91I GGTNACC 1 cut(s) 552
EcoNI CCTNNNNNAGG 2 cut(s) 546, 740
EcoO109I RGGNCCY 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 552
EcoRII CCWGG 1 cut(s) 142
EcoRV GATATC 1 cut(s) 700
EcoT14I CCWWGG 1 cut(s) 240
EgeI GGCGCC 1 cut(s) 711
EheI GGCGCC 1 cut(s) 711
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 4 cut(s) 95, 244, 257, 879
FaqI GGGAC 1 cut(s) 718
FatI CATG 4 cut(s) 91, 240, 253, 875
FblI GTMKAC 1 cut(s) 858
Fnu4HI GCNGC 5 cut(s) 162, 270, 364, 597, 807
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 5 cut(s) 162, 270, 364, 597, 807
FspBI CTAG 2 cut(s) 480, 870
GlaI GCGC 2 cut(s) 209, 711
GluI GCNGC 5 cut(s) 162, 270, 364, 597, 807
HaeII RGCGCY 1 cut(s) 713
HaeIII GGCC 1 cut(s) 648
HapII CCGG 4 cut(s) 194, 429, 557, 713
HhaI GCGC 2 cut(s) 210, 712
Hin1I GRCGYC 1 cut(s) 710
Hin1II CATG 4 cut(s) 95, 244, 257, 879
Hin6I GCGC 2 cut(s) 208, 710
HinP1I GCGC 2 cut(s) 208, 710
HinfI GANTC 2 cut(s) 100, 791
HpaII CCGG 4 cut(s) 194, 429, 557, 713
HphI GGTGA 4 cut(s) 389, 546, 672, 754
Hpy166II GTNNAC 2 cut(s) 782, 859
Hpy188I TCNGA 3 cut(s) 750, 823, 849
Hpy188III TCNNGA 6 cut(s) 194, 231, 396, 513, 542, 795
Hpy8I GTNNAC 2 cut(s) 782, 859
HpyAV CCTTC 4 cut(s) 91, 295, 585, 736
HpyCH4III ACNGT 4 cut(s) 499, 616, 838, 865
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 5 cut(s) 65, 164, 272, 477, 653
HpyF10VI GCNNNNNNNGC 1 cut(s) 593
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 710
Hsp92II CATG 4 cut(s) 95, 244, 257, 879
HspAI GCGC 2 cut(s) 208, 710
KasI GGCGCC 1 cut(s) 709
Kpn2I TCCGGA 1 cut(s) 193
KpnI GGTACC 1 cut(s) 869
Kzo9I GATC 3 cut(s) 16, 55, 745
LmnI GCTCC 2 cut(s) 584, 774
Lsp1109I GCAGC 5 cut(s) 148, 256, 375, 608, 793
LweI GCATC 1 cut(s) 455
MaeI CTAG 2 cut(s) 480, 870
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 552, 760
MalI GATC 3 cut(s) 18, 57, 747
MboI GATC 3 cut(s) 16, 55, 745
MboII GAAGA 2 cut(s) 209, 467
MflI RGATCY 2 cut(s) 16, 55
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 7 cut(s) 105, 153, 189, 380, 568, 620, 785
Mly113I GGCGCC 1 cut(s) 710
MnlI CCTC 4 cut(s) 88, 261, 331, 736
MroI TCCGGA 1 cut(s) 193
MseI TTAA 7 cut(s) 35, 134, 213, 279, 567, 618, 636
MspI CCGG 4 cut(s) 194, 429, 557, 713
MspR9I CCNGG 3 cut(s) 144, 429, 430
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 593
NarI GGCGCC 1 cut(s) 710
NciI CCSGG 2 cut(s) 429, 430
NcoI CCATGG 1 cut(s) 240
NdeII GATC 3 cut(s) 16, 55, 745
NlaIII CATG 4 cut(s) 95, 244, 257, 879
NlaIV GGNNCC 3 cut(s) 606, 711, 867
NmuCI GTSAC 2 cut(s) 552, 760
NspV TTCGAA 1 cut(s) 789
PceI AGGCCT 1 cut(s) 648
PfeI GAWTC 2 cut(s) 100, 791
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 5 cut(s) 163, 271, 365, 598, 808
PluTI GGCGCC 1 cut(s) 713
PpuMI RGGWCCY 1 cut(s) 539
Psp5II RGGWCCY 1 cut(s) 539
Psp6I CCWGG 1 cut(s) 142
PspEI GGTNACC 1 cut(s) 552
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 3 cut(s) 606, 711, 867
PspPI GGNCC 2 cut(s) 95, 539
PspPPI RGGWCCY 1 cut(s) 539
PstNI CAGNNNCTG 1 cut(s) 542
PsuI RGATCY 2 cut(s) 16, 55
RsaI GTAC 1 cut(s) 867
RsaNI GTAC 1 cut(s) 866
SaqAI TTAA 7 cut(s) 35, 134, 213, 279, 567, 618, 636
SatI GCNGC 5 cut(s) 162, 270, 364, 597, 807
Sau3AI GATC 3 cut(s) 16, 55, 745
Sau96I GGNCC 2 cut(s) 95, 539
ScrFI CCNGG 3 cut(s) 144, 429, 430
SduI GDGCHC 1 cut(s) 142
SetI ASST 9 cut(s) 70, 271, 279, 287, 541, 589, 610, 755, 871
SfaNI GCATC 1 cut(s) 455
SfcI CTRYAG 1 cut(s) 534
SfoI GGCGCC 1 cut(s) 711
SfuI TTCGAA 1 cut(s) 789
SgrAI CRCCGGYG 1 cut(s) 556
SinI GGWCC 2 cut(s) 95, 539
SmaI CCCGGG 1 cut(s) 430
Sse9I AATT 7 cut(s) 105, 153, 189, 380, 568, 620, 785
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 1 cut(s) 336
SspDI GGCGCC 1 cut(s) 709
SspMI CTAG 2 cut(s) 480, 870
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 3 cut(s) 142, 427, 428
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 4 cut(s) 499, 616, 838, 865
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 578, 789
TaqII GACCGA 1 cut(s) 112
TasI AATT 7 cut(s) 105, 153, 189, 380, 568, 620, 785
TfiI GAWTC 2 cut(s) 100, 791
Tru1I TTAA 7 cut(s) 35, 134, 213, 279, 567, 618, 636
Tru9I TTAA 7 cut(s) 35, 134, 213, 279, 567, 618, 636
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 2 cut(s) 552, 760
TseI GCWGC 5 cut(s) 161, 269, 363, 596, 806
Tsp45I GTSAC 2 cut(s) 552, 760
TspDTI ATGAA 3 cut(s) 17, 456, 504
TspMI CCCGGG 1 cut(s) 428
TspRI CASTG 1 cut(s) 265
VpaK11BI GGWCC 2 cut(s) 95, 539
XagI CCTNNNNNAGG 2 cut(s) 546, 740
XapI RAATTY 3 cut(s) 153, 380, 620
XmaI CCCGGG 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 858
XspI CTAG 2 cut(s) 480, 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.