Rh5BG456000

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001233
Physical Location & Seq
Forward (+)
72989288 .. 73009265
19978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG456000.1

Sequence Viewer

Length: 612 bp
ATGAATGATCCCAGCAGCCATGTTTCCACTGGTGAATTCGCTTATGGATCAGGCCATATCAATCCATTAAAAGCTATTGACCCCGGGCTTGTGTATGATGCTTCCAAAGAAGATTACATAAAGTTGCTGTGCATCAGCTATGATGAAAGCAGAGTTAGACTTGTATCAGGAGATAACAGCACATGCCCTACAGGTCCTGACAAGGGATCTCCGAAGGATCATAATTACCCTTCGATGGGAGCTAAAGTTACCGCAATGCAACCATTTACAGTTAAATTTAATAGAAGAGTTAAAAATGTTGGCAATGCAAACTCCACTTATGTAGCCAAAATCTCCCCCAATTCCAAACTCGAGATTAAAGTGGTGCCGGAAGTCCTATCCTTCAAGTCCTTGAATGAGGAGAAGACTTTTAATGTGACTGTTTCTGGAAGCAATTTGCCTTTTGAATCACGAGCATCTGCTTCACTGCAAAACCCTTGCCTCATCATCAATGGGCAAATCCTCCTACCAAACCTACCGGCGTCGAACTCAAACCTACCGGAAATAGCTGGAGCTCCCGGAGGACGTCACGTCTTCCATACTCTCCGCCTCGGCGCCATCAAAATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

203

Amino Acids

21.81

Weight (kDa)

8.94

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 73 - 150 8.1e-18 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 568
AccB1I GGYRCC 2 cut(s) 364, 593
AciI CCGC 2 cut(s) 252, 586
AclWI GGATC 4 cut(s) 2, 55, 214, 225
AcsI RAATTY 2 cut(s) 35, 275
AcyI GRCGYC 3 cut(s) 521, 565, 594
AfiI CCNNNNNNNGG 3 cut(s) 203, 235, 236
AgsI TTSAA 3 cut(s) 385, 394, 446
AhdI GACNNNNNGTC 1 cut(s) 569
AjiI CACGTC 1 cut(s) 571
AluBI AGCT 5 cut(s) 74, 138, 242, 548, 554
AluI AGCT 5 cut(s) 74, 138, 242, 548, 554
Alw21I GWGCWC 1 cut(s) 556
AlwI GGATC 4 cut(s) 2, 55, 214, 225
AlwNI CAGNNNCTG 1 cut(s) 197
Ama87I CYCGRG 2 cut(s) 83, 350
AoxI GGCC 1 cut(s) 52
ApeKI GCWGC 1 cut(s) 15
ApoI RAATTY 2 cut(s) 35, 275
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 1 cut(s) 194
AsuC2I CCSGG 3 cut(s) 84, 85, 558
AsuHPI GGTGA 1 cut(s) 44
AvaI CYCGRG 2 cut(s) 83, 350
AvaII GGWCC 1 cut(s) 194
BanI GGYRCC 2 cut(s) 364, 593
BanII GRGCYC 1 cut(s) 556
BauI CACGAG 1 cut(s) 450
BbsI GAAGAC 2 cut(s) 410, 565
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 1 cut(s) 27
BccI CCATC 2 cut(s) 229, 605
BcnI CCSGG 3 cut(s) 84, 85, 558
BfmI CTRYAG 1 cut(s) 189
BfoI RGCGCY 1 cut(s) 597
BisI GCNGC 1 cut(s) 16
BlsI GCNGC 1 cut(s) 17
Bme1390I CCNGG 3 cut(s) 84, 85, 558
Bme18I GGWCC 1 cut(s) 194
BmeRI GACNNNNNGTC 1 cut(s) 569
BmeT110I CYCGRG 2 cut(s) 83, 350
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 2 cut(s) 366, 595
BmrFI CCNGG 3 cut(s) 84, 85, 558
BmsI GCATC 3 cut(s) 88, 141, 464
BpiI GAAGAC 2 cut(s) 410, 565
BpmI CTGGAG 1 cut(s) 570
BpuMI CCSGG 3 cut(s) 84, 85, 558
BsaHI GRCGYC 3 cut(s) 521, 565, 594
BsaJI CCNNGG 3 cut(s) 82, 83, 589
BsaWI WCCGGW 1 cut(s) 538
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 235, 236
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 2 cut(s) 261, 310
BseDI CCNNGG 3 cut(s) 82, 83, 589
BseLI CCNNNNNNNGG 3 cut(s) 203, 235, 236
BseMI GCAATG 2 cut(s) 261, 310
BseNI ACTGG 1 cut(s) 34
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 1 cut(s) 27
BseYI CCCAGC 1 cut(s) 11
BshFI GGCC 1 cut(s) 54
BshNI GGYRCC 2 cut(s) 364, 593
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 2 cut(s) 83, 350
BsiSI CCGG 5 cut(s) 84, 368, 518, 539, 558
BslI CCNNNNNNNGG 3 cut(s) 203, 235, 236
BsnI GGCC 1 cut(s) 54
BsoBI CYCGRG 2 cut(s) 83, 350
Bsp1286I GDGCHC 1 cut(s) 556
Bsp143I GATC 4 cut(s) 7, 47, 206, 217
BspACI CCGC 2 cut(s) 252, 586
BspANI GGCC 1 cut(s) 54
BspLI GGNNCC 2 cut(s) 366, 595
BspPI GGATC 4 cut(s) 2, 55, 214, 225
BspT107I GGYRCC 2 cut(s) 364, 593
BsrDI GCAATG 2 cut(s) 261, 310
BsrFI RCCGGY 1 cut(s) 517
BsrI ACTGG 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 3 cut(s) 82, 83, 589
BssMI GATC 4 cut(s) 7, 47, 206, 217
BssNI GRCGYC 3 cut(s) 521, 565, 594
BssSI CACGAG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 450
Bst4CI ACNGT 2 cut(s) 271, 421
Bst6I CTCTTC 1 cut(s) 280
BstACI GRCGYC 3 cut(s) 521, 565, 594
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 4 cut(s) 10, 50, 209, 220
BstMBI GATC 4 cut(s) 7, 47, 206, 217
BstNSI RCATGY 1 cut(s) 186
BstSCI CCNGG 3 cut(s) 82, 83, 556
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 27
BstV2I GAAGAC 2 cut(s) 410, 565
BstX2I RGATCY 1 cut(s) 206
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 1 cut(s) 206
BsuRI GGCC 1 cut(s) 54
BtrI CACGTC 1 cut(s) 571
BtsI GCAGTG 1 cut(s) 464
BtsIMutI CAGTG 2 cut(s) 27, 464
CaiI CAGNNNCTG 1 cut(s) 197
CfoI GCGC 1 cut(s) 596
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 194
Cfr9I CCCGGG 1 cut(s) 83
CseI GACGC 1 cut(s) 510
CviAII CATG 2 cut(s) 20, 183
DinI GGCGCC 1 cut(s) 595
DpnI GATC 4 cut(s) 9, 49, 208, 219
DpnII GATC 4 cut(s) 7, 47, 206, 217
DriI GACNNNNNGTC 1 cut(s) 569
Eam1104I CTCTTC 1 cut(s) 280
Eam1105I GACNNNNNGTC 1 cut(s) 569
EarI CTCTTC 1 cut(s) 280
EciI GGCGGA 1 cut(s) 575
Ecl136II GAGCTC 1 cut(s) 554
Eco24I GRGCYC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 554
Eco88I CYCGRG 2 cut(s) 83, 350
EcoICRI GAGCTC 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 194
EcoRI GAATTC 1 cut(s) 35
EcoT38I GRGCYC 1 cut(s) 556
EgeI GGCGCC 1 cut(s) 595
EheI GGCGCC 1 cut(s) 595
FaeI CATG 2 cut(s) 23, 186
FatI CATG 2 cut(s) 19, 182
Fnu4HI GCNGC 1 cut(s) 16
FriOI GRGCYC 1 cut(s) 556
Fsp4HI GCNGC 1 cut(s) 16
GlaI GCGC 1 cut(s) 595
GluI GCNGC 1 cut(s) 16
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 1 cut(s) 570
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 1 cut(s) 54
HapII CCGG 5 cut(s) 84, 368, 518, 539, 558
HgaI GACGC 1 cut(s) 510
HhaI GCGC 1 cut(s) 596
Hin1I GRCGYC 3 cut(s) 521, 565, 594
Hin1II CATG 2 cut(s) 23, 186
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HinfI GANTC 1 cut(s) 446
HpaII CCGG 5 cut(s) 84, 368, 518, 539, 558
HphI GGTGA 1 cut(s) 44
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 5 cut(s) 168, 197, 352, 426, 450
Hpy99I CGWCG 1 cut(s) 526
HpyAV CCTTC 3 cut(s) 208, 240, 391
HpyCH4III ACNGT 2 cut(s) 271, 421
HpyCH4IV ACGT 2 cut(s) 565, 570
HpyCH4V TGCA 4 cut(s) 132, 259, 308, 469
HpySE526I ACGT 2 cut(s) 565, 570
Hsp92I GRCGYC 3 cut(s) 521, 565, 594
Hsp92II CATG 2 cut(s) 23, 186
HspAI GCGC 1 cut(s) 594
KasI GGCGCC 1 cut(s) 593
Kzo9I GATC 4 cut(s) 7, 47, 206, 217
LmnI GCTCC 3 cut(s) 239, 551, 559
Lsp1109I GCAGC 1 cut(s) 27
LweI GCATC 3 cut(s) 88, 141, 464
MaeII ACGT 2 cut(s) 565, 570
MaeIII GTNAC 3 cut(s) 247, 415, 566
MalI GATC 4 cut(s) 9, 49, 208, 219
MboI GATC 4 cut(s) 7, 47, 206, 217
MboII GAAGA 4 cut(s) 122, 297, 415, 565
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 556
MluCI AATT 5 cut(s) 35, 223, 275, 340, 433
Mly113I GGCGCC 1 cut(s) 594
MnlI CCTC 5 cut(s) 391, 491, 512, 554, 599
MseI TTAA 6 cut(s) 68, 273, 279, 291, 357, 411
MslI CAYNNNNRTG 1 cut(s) 602
MspI CCGG 5 cut(s) 84, 368, 518, 539, 558
MspR9I CCNGG 3 cut(s) 84, 85, 558
NarI GGCGCC 1 cut(s) 594
NciI CCSGG 3 cut(s) 84, 85, 558
NdeII GATC 4 cut(s) 7, 47, 206, 217
NlaIII CATG 2 cut(s) 23, 186
NlaIV GGNNCC 2 cut(s) 366, 595
NmeAIII GCCGAG 1 cut(s) 570
NmuCI GTSAC 2 cut(s) 415, 566
NspI RCATGY 1 cut(s) 186
PaeR7I CTCGAG 1 cut(s) 350
PfeI GAWTC 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 556
PkrI GCNGC 1 cut(s) 17
PluTI GGCGCC 1 cut(s) 597
PpuMI RGGWCCY 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 556
Psp5II RGGWCCY 1 cut(s) 194
PspFI CCCAGC 1 cut(s) 11
PspN4I GGNNCC 2 cut(s) 366, 595
PspPI GGNCC 1 cut(s) 194
PspPPI RGGWCCY 1 cut(s) 194
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 1 cut(s) 206
RseI CAYNNNNRTG 1 cut(s) 602
SacI GAGCTC 1 cut(s) 556
SaqAI TTAA 6 cut(s) 68, 273, 279, 291, 357, 411
SatI GCNGC 1 cut(s) 16
Sau3AI GATC 4 cut(s) 7, 47, 206, 217
Sau96I GGNCC 1 cut(s) 194
ScrFI CCNGG 3 cut(s) 84, 85, 558
SduI GDGCHC 1 cut(s) 556
SfaNI GCATC 3 cut(s) 88, 141, 464
SfcI CTRYAG 1 cut(s) 189
SfoI GGCGCC 1 cut(s) 595
Sfr274I CTCGAG 1 cut(s) 350
SinI GGWCC 1 cut(s) 194
SlaI CTCGAG 1 cut(s) 350
SmaI CCCGGG 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 602
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 5 cut(s) 35, 223, 275, 340, 433
SsiI CCGC 2 cut(s) 252, 586
SspDI GGCGCC 1 cut(s) 593
SstI GAGCTC 1 cut(s) 556
StyD4I CCNGG 3 cut(s) 82, 83, 556
TaaI ACNGT 2 cut(s) 271, 421
TaiI ACGT 2 cut(s) 568, 573
TaqI TCGA 3 cut(s) 233, 351, 524
TasI AATT 5 cut(s) 35, 223, 275, 340, 433
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 6 cut(s) 68, 273, 279, 291, 357, 411
Tru9I TTAA 6 cut(s) 68, 273, 279, 291, 357, 411
TscAI CASTG 2 cut(s) 34, 471
TseFI GTSAC 2 cut(s) 415, 566
TseI GCWGC 1 cut(s) 15
Tsp45I GTSAC 2 cut(s) 415, 566
TspDTI ATGAA 2 cut(s) 17, 159
TspMI CCCGGG 1 cut(s) 83
TspRI CASTG 2 cut(s) 34, 471
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 2 cut(s) 35, 275
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XhoI CTCGAG 1 cut(s) 350
XmaI CCCGGG 1 cut(s) 83
ZraI GACGTC 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.