Rh5BG456200

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001233
Physical Location & Seq
Forward (+)
73012706 .. 73017065
4360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG456200.1

Sequence Viewer

Length: 1443 bp
ATGAAGACAGAAAGGTCCATATTGTGTACATGGGGTCACTTCCATATAAGGAGTACTCTCCATTTTCTCATCACCATGGTATGCTACAGACTGTTGTCCACAGCAGATATGAAGGAAGTAGTATCTGTCTTCCCGATCACAGCATTACAACTTCAAACAAGAAGATCTTGGGACTTCATGGGTTTCACTGAGAAAATTCATAGACGAGGAAATGTTGAGAGTGATATTATTGTGGATGCAGTGATTGACTCTGGAATTTGGCCTGAATCAGAAAGCTTCAATGACGAAGTTTTTGGTCCTCCTCCTAAGAAGTGGAAAGGTGCTTGTATGGGCGGCTTCAATTTCACTTGCAACAAGTTATGTGATGAAGATTGCCTTGACAGTGATTCAGTTAAGGGAAAGATTGTGCTATGTGATGCATTCGCTGAAGCAGATTTTGTGGCTCATAAAGCTGGTGCACTAGGTTCTATTTTAAATAATGACGGAACTGGTGATTTTTCTAATGTTGTCTCATTACCTGCAATAGCTTTAAGCTATGAAAACTATAATGCAGTCAAGTTTTACATGAACTCCACTAGAGATCCTCGAGCAAACATATTAAGAAGTGAAGTCATAAATGACCCGGCTGCACCAATTGTCGCTTCCTTCTCTTCACGTGGACCCAATCCAATTACACCTGACATTATAAAGCCAGATATAACTGCGCCTGGTGTAGATATTTTGGCTGCATATTCGACTGTTGCCTCAATTTCAGAGAGCCCCGAAGACAAGAGGCATGTAAAGTACCATATATTATCTGGAACCTCCATGTCTTGCCCTCATGCTGCTGCTGTTGCTGCGTATGTTAAATCATTCCATCCCAATTGGTCTCCATCAGCTATAAAATCATCTCTTATGACTACTGCTTGGCCAATGAATAATACCAACAATGCACCCGCTGAGTTTGCTTATGGATCTGGACATGTCAATCCTGTAAAAGCTATAGATCCCGGGCTTGTTTATGAAACTTCTAAAGAAGATTTCATAAACCTACTTTGCATGGACTATGATGAAGCCAAAGTGAGACTGATATCAGGAGATAGCAGCACTTGCCCTACAGGCTCTGATAAAGGATCACCAAAGGATCTTAACTACCCTTCAATGGCTGCAGCAAACATTACACCAAAGAAACCTTTTACGATTAACTTTCATAGAACAGTTAAAAATGTTGGCCTTGCAAACTCTACTTACAATGCCACCATCTTCCAAAGTTCCAAAGACGTGGATATCAAAGTTGTGCCTCAATTTCTATCCTTCAAGTCCTTGAATGAGGAGAAGAGCTTTGATGTGGCTGTTGTTGGAAAAGGTTTGATAGGTGGATCACTAATTTCTTCGTCACTGGTTTGGTCTGATGGTGTTCATAACGTTAGGAGTCCAGTTGTGGTGCGTACTGAAATAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

480

Amino Acids

52.25

Weight (kDa)

6.07

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 124 - 184 4.6e-06 PA domain
Peptidase_S8 PF00082 213 - 320 3.5e-20 Subtilase family
fn3_6 PF17766 375 - 475 2.2e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 686
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 526
AciI CCGC 2 cut(s) 333, 936
AclI AACGTT 1 cut(s) 1404
AclWI GGATC 6 cut(s) 575, 961, 980, 1120, 1131, 1366
AcoI YGGCCR 1 cut(s) 908
AcsI RAATTY 2 cut(s) 195, 255
AcuI CTGAAG 1 cut(s) 447
AcvI CACGTG 1 cut(s) 656
AfaI GTAC 4 cut(s) 28, 55, 785, 1429
AfiI CCNNNNNNNGG 1 cut(s) 1141
AflIII ACRYGT 1 cut(s) 961
AgsI TTSAA 6 cut(s) 155, 280, 340, 1140, 1297, 1306
AjiI CACGTC 1 cut(s) 1261
AjnI CCWGG 1 cut(s) 706
AjuI GAANNNNNNNTTGG 2 cut(s) 625, 657
AluBI AGCT 7 cut(s) 276, 452, 527, 534, 878, 980, 1320
AluI AGCT 7 cut(s) 276, 452, 527, 534, 878, 980, 1320
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 3 cut(s) 514, 873, 1057
Alw44I GTGCAC 1 cut(s) 456
AlwI GGATC 6 cut(s) 575, 961, 980, 1120, 1131, 1366
AlwNI CAGNNNCTG 1 cut(s) 1103
Ama87I CYCGRG 2 cut(s) 585, 989
AoxI GGCC 3 cut(s) 260, 908, 1210
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 8 cut(s) 626, 725, 824, 827, 836, 1083, 1145, 1148
ApoI RAATTY 2 cut(s) 195, 255
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspLEI GCGC 1 cut(s) 706
AspS9I GGNCC 3 cut(s) 15, 296, 659
AsuC2I CCSGG 3 cut(s) 623, 990, 991
AsuHPI GGTGA 3 cut(s) 64, 503, 1107
AvaI CYCGRG 2 cut(s) 585, 989
AvaII GGWCC 3 cut(s) 15, 296, 659
BaeGI GKGCMC 1 cut(s) 460
BalI TGGCCA 1 cut(s) 910
BanII GRGCYC 1 cut(s) 761
BbrPI CACGTG 1 cut(s) 656
BbsI GAAGAC 3 cut(s) 11, 121, 771
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 8 cut(s) 613, 712, 811, 814, 823, 1095, 1132, 1160
BccI CCATC 4 cut(s) 864, 880, 1247, 1385
BciT130I CCWGG 1 cut(s) 708
BcnI CCSGG 3 cut(s) 623, 990, 991
BcoDI GTCTC 3 cut(s) 514, 873, 1057
BfaI CTAG 2 cut(s) 461, 576
BfmI CTRYAG 4 cut(s) 85, 981, 1095, 1146
BfuAI ACCTGC 1 cut(s) 526
BglI GCCNNNNNGGC 1 cut(s) 1098
BglII AGATCT 1 cut(s) 164
BisI GCNGC 9 cut(s) 334, 627, 726, 825, 828, 837, 1084, 1146, 1149
BlsI GCNGC 9 cut(s) 335, 628, 727, 826, 829, 838, 1085, 1147, 1150
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 4 cut(s) 623, 708, 990, 991
Bme18I GGWCC 3 cut(s) 15, 296, 659
BmeT110I CYCGRG 2 cut(s) 585, 989
BmgBI CACGTC 1 cut(s) 1261
BmgT120I GGNCC 3 cut(s) 15, 296, 659
BmiI GGNNCC 2 cut(s) 661, 802
BmrFI CCNGG 4 cut(s) 623, 708, 990, 991
BmsI GCATC 2 cut(s) 226, 406
BoxI GACNNNNGTC 1 cut(s) 94
BpiI GAAGAC 3 cut(s) 11, 121, 771
BpuMI CCSGG 3 cut(s) 623, 990, 991
BsaAI YACGTR 1 cut(s) 656
BsaI GGTCTC 1 cut(s) 873
BsaJI CCNNGG 2 cut(s) 75, 989
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 1 cut(s) 1141
Bse1I ACTGG 3 cut(s) 493, 1383, 1415
BseBI CCWGG 1 cut(s) 708
BseDI CCNNGG 2 cut(s) 75, 989
BseGI GGATG 2 cut(s) 241, 856
BseLI CCNNNNNNNGG 1 cut(s) 1141
BseMII CTCAG 2 cut(s) 180, 930
BseNI ACTGG 3 cut(s) 493, 1383, 1415
BseRI GAGGAG 2 cut(s) 291, 1325
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 8 cut(s) 613, 712, 811, 814, 823, 1095, 1132, 1160
BsgI GTGCAG 1 cut(s) 612
BshFI GGCC 3 cut(s) 262, 910, 1212
BsiHKAI GWGCWC 1 cut(s) 460
BsiHKCI CYCGRG 2 cut(s) 585, 989
BsiSI CCGG 2 cut(s) 623, 990
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 1 cut(s) 1141
BsmAI GTCTC 3 cut(s) 514, 873, 1057
BsmFI GGGAC 1 cut(s) 185
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 3 cut(s) 262, 910, 1212
Bso31I GGTCTC 1 cut(s) 873
BsoBI CYCGRG 2 cut(s) 585, 989
Bsp1286I GDGCHC 2 cut(s) 460, 761
Bsp1407I TGTACA 1 cut(s) 26
Bsp143I GATC 8 cut(s) 135, 164, 580, 953, 985, 1112, 1123, 1358
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 2 cut(s) 333, 936
BspANI GGCC 3 cut(s) 262, 910, 1212
BspCNI CTCAG 2 cut(s) 181, 931
BspLI GGNNCC 2 cut(s) 661, 802
BspMAI CTGCAG 1 cut(s) 1150
BspMI ACCTGC 1 cut(s) 526
BspPI GGATC 6 cut(s) 575, 961, 980, 1120, 1131, 1366
BspQI GCTCTTC 1 cut(s) 1310
BspTNI GGTCTC 1 cut(s) 873
BsrGI TGTACA 1 cut(s) 26
BsrI ACTGG 3 cut(s) 493, 1383, 1415
BssECI CCNNGG 2 cut(s) 75, 989
BssMI GATC 8 cut(s) 135, 164, 580, 953, 985, 1112, 1123, 1358
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 4 cut(s) 93, 383, 739, 1198
Bst6I CTCTTC 2 cut(s) 655, 1310
BstAPI GCANNNNNTGC 1 cut(s) 1089
BstAUI TGTACA 1 cut(s) 26
BstBAI YACGTR 1 cut(s) 656
BstDEI CTNAG 3 cut(s) 189, 306, 939
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 2 cut(s) 241, 856
BstHHI GCGC 1 cut(s) 706
BstKTI GATC 8 cut(s) 138, 167, 583, 956, 988, 1115, 1126, 1361
BstMAI GTCTC 3 cut(s) 514, 873, 1057
BstMBI GATC 8 cut(s) 135, 164, 580, 953, 985, 1112, 1123, 1358
BstMWI GCNNNNNNNGC 6 cut(s) 449, 833, 836, 944, 1089, 1098
BstNI CCWGG 1 cut(s) 708
BstNSI RCATGY 2 cut(s) 779, 965
BstPAI GACNNNNGTC 1 cut(s) 94
BstSCI CCNGG 4 cut(s) 621, 706, 988, 989
BstSFI CTRYAG 4 cut(s) 85, 981, 1095, 1146
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 8 cut(s) 613, 712, 811, 814, 823, 1095, 1132, 1160
BstV2I GAAGAC 3 cut(s) 11, 121, 771
BstX2I RGATCY 5 cut(s) 164, 580, 953, 985, 1123
BstXI CCANNNNNNTGG 1 cut(s) 1261
BstYI RGATCY 5 cut(s) 164, 580, 953, 985, 1123
BsuRI GGCC 3 cut(s) 262, 910, 1212
BtgI CCRYGG 1 cut(s) 75
BtrI CACGTC 1 cut(s) 1261
BtsCI GGATG 2 cut(s) 241, 856
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 4 cut(s) 186, 246, 388, 1376
BveI ACCTGC 1 cut(s) 526
CaiI CAGNNNCTG 1 cut(s) 1103
CfoI GCGC 1 cut(s) 706
Cfr13I GGNCC 3 cut(s) 15, 296, 659
Cfr9I CCCGGG 1 cut(s) 989
Csp6I GTAC 4 cut(s) 27, 54, 784, 1428
CviAII CATG 9 cut(s) 30, 76, 178, 565, 776, 808, 821, 962, 1039
CviQI GTAC 4 cut(s) 27, 54, 784, 1428
DdeI CTNAG 3 cut(s) 189, 306, 939
DpnI GATC 8 cut(s) 137, 166, 582, 955, 987, 1114, 1125, 1360
DpnII GATC 8 cut(s) 135, 164, 580, 953, 985, 1112, 1123, 1358
DraI TTTAAA 1 cut(s) 474
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
EaeI YGGCCR 1 cut(s) 908
Eam1104I CTCTTC 2 cut(s) 655, 1310
EarI CTCTTC 2 cut(s) 655, 1310
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 761
Eco31I GGTCTC 1 cut(s) 873
Eco32I GATATC 2 cut(s) 1071, 1267
Eco47I GGWCC 3 cut(s) 15, 296, 659
Eco57I CTGAAG 1 cut(s) 447
Eco72I CACGTG 1 cut(s) 656
Eco88I CYCGRG 2 cut(s) 585, 989
EcoRII CCWGG 1 cut(s) 706
EcoRV GATATC 2 cut(s) 1071, 1267
EcoT14I CCWWGG 1 cut(s) 75
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 761
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 9 cut(s) 33, 79, 181, 568, 779, 811, 824, 965, 1042
FalI AAGNNNNNCTT 4 cut(s) 151, 183, 360, 392
FaqI GGGAC 1 cut(s) 185
FatI CATG 9 cut(s) 29, 75, 177, 564, 775, 807, 820, 961, 1038
FauI CCCGC 1 cut(s) 943
Fnu4HI GCNGC 9 cut(s) 334, 627, 726, 825, 828, 837, 1084, 1146, 1149
FokI GGATG 2 cut(s) 248, 843
FriOI GRGCYC 1 cut(s) 761
Fsp4HI GCNGC 9 cut(s) 334, 627, 726, 825, 828, 837, 1084, 1146, 1149
FspBI CTAG 2 cut(s) 461, 576
GlaI GCGC 1 cut(s) 705
GluI GCNGC 9 cut(s) 334, 627, 726, 825, 828, 837, 1084, 1146, 1149
HaeIII GGCC 3 cut(s) 262, 910, 1212
HapII CCGG 2 cut(s) 623, 990
HhaI GCGC 1 cut(s) 706
Hin1II CATG 9 cut(s) 33, 79, 181, 568, 779, 811, 824, 965, 1042
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HindIII AAGCTT 1 cut(s) 274
HinfI GANTC 4 cut(s) 248, 266, 386, 1411
HpaII CCGG 2 cut(s) 623, 990
HphI GGTGA 3 cut(s) 64, 503, 1107
Hpy166II GTNNAC 4 cut(s) 27, 99, 458, 659
Hpy188I TCNGA 4 cut(s) 271, 754, 1105, 1390
Hpy188III TCNNGA 5 cut(s) 133, 252, 798, 957, 1074
Hpy8I GTNNAC 4 cut(s) 27, 99, 458, 659
HpyAV CCTTC 4 cut(s) 106, 655, 1146, 1303
HpyCH4III ACNGT 4 cut(s) 93, 383, 739, 1198
HpyCH4IV ACGT 3 cut(s) 655, 1260, 1404
HpyF10VI GCNNNNNNNGC 6 cut(s) 449, 833, 836, 944, 1089, 1098
HpyF3I CTNAG 3 cut(s) 189, 306, 939
HpySE526I ACGT 3 cut(s) 655, 1260, 1404
Hsp92II CATG 9 cut(s) 33, 79, 181, 568, 779, 811, 824, 965, 1042
HspAI GCGC 1 cut(s) 704
Kzo9I GATC 8 cut(s) 135, 164, 580, 953, 985, 1112, 1123, 1358
LguI GCTCTTC 1 cut(s) 1310
Lsp1109I GCAGC 8 cut(s) 613, 712, 811, 814, 823, 1095, 1132, 1160
LweI GCATC 2 cut(s) 226, 406
MaeI CTAG 2 cut(s) 461, 576
MaeII ACGT 3 cut(s) 655, 1260, 1404
MaeIII GTNAC 2 cut(s) 35, 1374
MalI GATC 8 cut(s) 137, 166, 582, 955, 987, 1114, 1125, 1360
MboI GATC 8 cut(s) 135, 164, 580, 953, 985, 1112, 1123, 1358
MfeI CAATTG 2 cut(s) 633, 862
MflI RGATCY 5 cut(s) 164, 580, 953, 985, 1123
MhlI GDGCHC 2 cut(s) 460, 761
MlsI TGGCCA 1 cut(s) 910
MluCI AATT 9 cut(s) 195, 255, 340, 633, 669, 747, 862, 1283, 1365
MluNI TGGCCA 1 cut(s) 910
MlyI GAGTC 2 cut(s) 242, 1420
MmeI TCCRAC 1 cut(s) 1318
Mox20I TGGCCA 1 cut(s) 910
Mph1103I ATGCAT 1 cut(s) 421
MscI TGGCCA 1 cut(s) 910
MseI TTAA 8 cut(s) 393, 473, 530, 599, 846, 1128, 1182, 1200
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 910
MspA1I CMGCKG 1 cut(s) 938
MspI CCGG 2 cut(s) 623, 990
MspR9I CCNGG 4 cut(s) 623, 708, 990, 991
MunI CAATTG 2 cut(s) 633, 862
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 1 cut(s) 708
MwoI GCNNNNNNNGC 6 cut(s) 449, 833, 836, 944, 1089, 1098
NciI CCSGG 3 cut(s) 623, 990, 991
NcoI CCATGG 1 cut(s) 75
NdeII GATC 8 cut(s) 135, 164, 580, 953, 985, 1112, 1123, 1358
NlaIII CATG 9 cut(s) 33, 79, 181, 568, 779, 811, 824, 965, 1042
NlaIV GGNNCC 2 cut(s) 661, 802
NmuCI GTSAC 2 cut(s) 35, 1374
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 2 cut(s) 779, 965
PaeR7I CTCGAG 1 cut(s) 585
PciI ACATGT 1 cut(s) 961
PciSI GCTCTTC 1 cut(s) 1310
PctI GAATGC 1 cut(s) 419
PfeI GAWTC 2 cut(s) 266, 386
PkrI GCNGC 9 cut(s) 335, 628, 727, 826, 829, 838, 1085, 1147, 1150
PleI GAGTC 2 cut(s) 242, 1419
PmaCI CACGTG 1 cut(s) 656
PmlI CACGTG 1 cut(s) 656
PpsI GAGTC 2 cut(s) 242, 1419
Ppu21I YACGTR 1 cut(s) 656
PscI ACATGT 1 cut(s) 961
PshAI GACNNNNGTC 1 cut(s) 94
PsiI TTATAA 1 cut(s) 686
Psp1406I AACGTT 1 cut(s) 1404
Psp6I CCWGG 1 cut(s) 706
PspCI CACGTG 1 cut(s) 656
PspGI CCWGG 1 cut(s) 706
PspN4I GGNNCC 2 cut(s) 661, 802
PspPI GGNCC 3 cut(s) 15, 296, 659
PspXI VCTCGAGB 1 cut(s) 585
PstI CTGCAG 1 cut(s) 1150
PstNI CAGNNNCTG 1 cut(s) 1103
PsuI RGATCY 5 cut(s) 164, 580, 953, 985, 1123
RsaI GTAC 4 cut(s) 28, 55, 785, 1429
RsaNI GTAC 4 cut(s) 27, 54, 784, 1428
RseI CAYNNNNRTG 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 1310
SaqAI TTAA 8 cut(s) 393, 473, 530, 599, 846, 1128, 1182, 1200
SatI GCNGC 9 cut(s) 334, 627, 726, 825, 828, 837, 1084, 1146, 1149
Sau3AI GATC 8 cut(s) 135, 164, 580, 953, 985, 1112, 1123, 1358
Sau96I GGNCC 3 cut(s) 15, 296, 659
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 2 cut(s) 242, 1420
ScrFI CCNGG 4 cut(s) 623, 708, 990, 991
SduI GDGCHC 2 cut(s) 460, 761
SfaNI GCATC 2 cut(s) 226, 406
SfcI CTRYAG 4 cut(s) 85, 981, 1095, 1146
Sfr274I CTCGAG 1 cut(s) 585
SinI GGWCC 3 cut(s) 15, 296, 659
SlaI CTCGAG 1 cut(s) 585
SmaI CCCGGG 1 cut(s) 991
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 585
SmoI CTYRAG 1 cut(s) 585
Sse9I AATT 9 cut(s) 195, 255, 340, 633, 669, 747, 862, 1283, 1365
SsiI CCGC 2 cut(s) 333, 936
SspMI CTAG 2 cut(s) 461, 576
StyD4I CCNGG 4 cut(s) 621, 706, 988, 989
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 4 cut(s) 93, 383, 739, 1198
TaiI ACGT 3 cut(s) 658, 1263, 1407
TaqI TCGA 2 cut(s) 586, 734
TasI AATT 9 cut(s) 195, 255, 340, 633, 669, 747, 862, 1283, 1365
TatI WGTACW 2 cut(s) 26, 53
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 2 cut(s) 266, 386
Tru1I TTAA 8 cut(s) 393, 473, 530, 599, 846, 1128, 1182, 1200
Tru9I TTAA 8 cut(s) 393, 473, 530, 599, 846, 1128, 1182, 1200
TscAI CASTG 4 cut(s) 193, 246, 388, 1383
TseFI GTSAC 2 cut(s) 35, 1374
TseI GCWGC 8 cut(s) 626, 725, 824, 827, 836, 1083, 1145, 1148
Tsp45I GTSAC 2 cut(s) 35, 1374
TspGWI ACGGA 1 cut(s) 498
TspMI CCCGGG 1 cut(s) 989
TspRI CASTG 4 cut(s) 193, 246, 388, 1383
VneI GTGCAC 1 cut(s) 456
VpaK11BI GGWCC 3 cut(s) 15, 296, 659
XapI RAATTY 2 cut(s) 195, 255
XceI RCATGY 2 cut(s) 779, 965
XcmI CCANNNNNNNNNTGG 1 cut(s) 794
XhoI CTCGAG 1 cut(s) 585
XmaI CCCGGG 1 cut(s) 989
XspI CTAG 2 cut(s) 461, 576
ZrmI AGTACT 1 cut(s) 55
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.