Rmu_co8485349.1_g000002

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8485349.1
OG0001233
Physical Location & Seq
Reverse (-)
1247 .. 1798
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8485349.1_g000002.1.cds

Sequence Viewer

Length: 552 bp
atgaattttacctacaatgattataatgccgctgctgaatttgcttgtggatctggacatatcaatcctgtcaaagctatagacccggggcttgtatatgaagcttctaaggaagactacataaagttgctctgcttggtcttaaaccagaccagtgttagacttatatcaggagataacagcacttgctctacaagctctgacaaaggatctttacatgatcaaaattacccatcgctgacagctgctgtcacaccaaagaaacctttttcattcaaatttcacagaaaagttcagaatgttggccttgcaaactccacctacaaggccaaaatattcgccggcaactctgctgatgaagctgacatcaaagtggtgcctgaagtcctttccttcaagtgcttgaatgaagagaagactttcgatgtgactgttgttggaaaaggtttgtcagaagaagtacaatcacatgtctctgcatcgctggtgtggtctgatgggattcatagcattagaagtccgattctcatccacagaagttttcttaattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

183

Amino Acids

19.97

Weight (kDa)

6.58

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 376
AciI CCGC 1 cut(s) 30
AclWI GGATC 2 cut(s) 58, 217
AcsI RAATTY 3 cut(s) 4, 38, 278
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 1 cut(s) 462
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 3 cut(s) 277, 397, 406
AluBI AGCT 5 cut(s) 77, 104, 198, 245, 362
AluI AGCT 5 cut(s) 77, 104, 198, 245, 362
Alw26I GTCTC 1 cut(s) 478
AlwI GGATC 2 cut(s) 58, 217
AlwNI CAGNNNCTG 1 cut(s) 248
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 2 cut(s) 304, 327
ApeKI GCWGC 2 cut(s) 32, 245
ApoI RAATTY 3 cut(s) 4, 38, 278
Asp700I GAANNNNTTC 1 cut(s) 419
AsuC2I CCSGG 2 cut(s) 86, 87
AvaI CYCGRG 1 cut(s) 85
BanI GGYRCC 1 cut(s) 376
BbsI GAAGAC 2 cut(s) 120, 422
BbvI GCAGC 2 cut(s) 19, 232
BccI CCATC 2 cut(s) 241, 491
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 2 cut(s) 86, 87
BcoDI GTCTC 1 cut(s) 478
BfmI CTRYAG 1 cut(s) 78
BisI GCNGC 3 cut(s) 30, 33, 246
BlsI GCNGC 3 cut(s) 31, 34, 247
Bme1390I CCNGG 2 cut(s) 86, 87
BmeT110I CYCGRG 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 378
BmrFI CCNGG 2 cut(s) 86, 87
BmsI GCATC 1 cut(s) 488
BpiI GAAGAC 2 cut(s) 120, 422
BpuMI CCSGG 2 cut(s) 86, 87
BsaBI GATNNNNATC 1 cut(s) 527
BsaJI CCNNGG 2 cut(s) 85, 86
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 153
Bse8I GATNNNNATC 1 cut(s) 527
BseDI CCNNGG 2 cut(s) 85, 86
BseGI GGATG 1 cut(s) 528
BseJI GATNNNNATC 1 cut(s) 527
BseNI ACTGG 1 cut(s) 153
BseXI GCAGC 2 cut(s) 19, 232
BshFI GGCC 2 cut(s) 306, 329
BshNI GGYRCC 1 cut(s) 376
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 2 cut(s) 86, 342
BsmAI GTCTC 1 cut(s) 478
BsnI GGCC 2 cut(s) 306, 329
BsoBI CYCGRG 1 cut(s) 85
Bsp143I GATC 3 cut(s) 50, 209, 220
BspACI CCGC 1 cut(s) 30
BspANI GGCC 2 cut(s) 306, 329
BspLI GGNNCC 1 cut(s) 378
BspPI GGATC 2 cut(s) 58, 217
BspT107I GGYRCC 1 cut(s) 376
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 153
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 85, 86
BssMI GATC 3 cut(s) 50, 209, 220
Bst4CI ACNGT 1 cut(s) 433
Bst6I CTCTTC 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 528
BstKTI GATC 3 cut(s) 53, 212, 223
BstMAI GTCTC 1 cut(s) 478
BstMBI GATC 3 cut(s) 50, 209, 220
BstMWI GCNNNNNNNGC 3 cut(s) 41, 195, 359
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 84, 85
BstSFI CTRYAG 1 cut(s) 78
BstV1I GCAGC 2 cut(s) 19, 232
BstV2I GAAGAC 2 cut(s) 120, 422
BstX2I RGATCY 2 cut(s) 50, 209
BstYI RGATCY 2 cut(s) 50, 209
BsuRI GGCC 2 cut(s) 306, 329
BtgZI GCGATG 2 cut(s) 219, 465
BtsCI GGATG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 343
CaiI CAGNNNCTG 1 cut(s) 248
Cfr10I RCCGGY 1 cut(s) 341
Cfr9I CCCGGG 1 cut(s) 85
Csp6I GTAC 1 cut(s) 461
CviAII CATG 2 cut(s) 218, 470
CviJI RGCY 8 cut(s) 77, 91, 104, 198, 245, 306, 329, 362
CviKI_1 RGCY 8 cut(s) 77, 91, 104, 198, 245, 306, 329, 362
CviQI GTAC 1 cut(s) 461
DdeI CTNAG 1 cut(s) 108
DpnI GATC 3 cut(s) 52, 211, 222
DpnII GATC 3 cut(s) 50, 209, 220
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco57I CTGAAG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 85
FaeI CATG 2 cut(s) 221, 473
FatI CATG 2 cut(s) 217, 469
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 30, 33, 246
FokI GGATG 1 cut(s) 515
Fsp4HI GCNGC 3 cut(s) 30, 33, 246
GluI GCNGC 3 cut(s) 30, 33, 246
HaeIII GGCC 2 cut(s) 306, 329
HapII CCGG 2 cut(s) 86, 342
Hin1II CATG 2 cut(s) 221, 473
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 2 cut(s) 502, 523
HpaII CCGG 2 cut(s) 86, 342
Hpy188I TCNGA 5 cut(s) 202, 297, 454, 496, 522
Hpy188III TCNNGA 2 cut(s) 54, 171
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4V TGCA 2 cut(s) 311, 479
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 195, 359
HpyF3I CTNAG 1 cut(s) 108
Hsp92II CATG 2 cut(s) 221, 473
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 3 cut(s) 50, 209, 220
LpnPI CCDG 9 cut(s) 39, 81, 99, 156, 161, 166, 355, 393, 470
Lsp1109I GCAGC 2 cut(s) 19, 232
LweI GCATC 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 250, 427
MalI GATC 3 cut(s) 52, 211, 222
MboI GATC 3 cut(s) 50, 209, 220
MboII GAAGA 4 cut(s) 125, 422, 427, 467
MflI RGATCY 2 cut(s) 50, 209
MluCI AATT 5 cut(s) 4, 38, 226, 278, 547
MmeI TCCRAC 1 cut(s) 418
MroNI GCCGGC 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 3 cut(s) 143, 546, 550
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 2 cut(s) 32, 245
MspI CCGG 2 cut(s) 86, 342
MspR9I CCNGG 2 cut(s) 86, 87
MwoI GCNNNNNNNGC 3 cut(s) 41, 195, 359
NaeI GCCGGC 1 cut(s) 343
NciI CCSGG 2 cut(s) 86, 87
NdeII GATC 3 cut(s) 50, 209, 220
NgoMIV GCCGGC 1 cut(s) 341
NlaIII CATG 2 cut(s) 221, 473
NlaIV GGNNCC 1 cut(s) 378
NmuCI GTSAC 2 cut(s) 250, 427
NspI RCATGY 1 cut(s) 473
PacI TTAATTAA 1 cut(s) 550
PciI ACATGT 1 cut(s) 469
PdiI GCCGGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 2 cut(s) 502, 523
PkrI GCNGC 3 cut(s) 31, 34, 247
PscI ACATGT 1 cut(s) 469
PsiI TTATAA 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 378
PstNI CAGNNNCTG 1 cut(s) 248
PsuI RGATCY 2 cut(s) 50, 209
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
RseI CAYNNNNRTG 1 cut(s) 371
SaqAI TTAA 3 cut(s) 143, 546, 550
SatI GCNGC 3 cut(s) 30, 33, 246
Sau3AI GATC 3 cut(s) 50, 209, 220
ScrFI CCNGG 2 cut(s) 86, 87
SetI ASST 9 cut(s) 14, 79, 106, 200, 247, 268, 323, 364, 448
SfaNI GCATC 1 cut(s) 488
SfcI CTRYAG 1 cut(s) 78
SmaI CCCGGG 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 371
Sse9I AATT 5 cut(s) 4, 38, 226, 278, 547
SsiI CCGC 1 cut(s) 30
SspI AATATT 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 84, 85
TaaI ACNGT 1 cut(s) 433
TaqI TCGA 1 cut(s) 423
TasI AATT 5 cut(s) 4, 38, 226, 278, 547
TatI WGTACW 1 cut(s) 460
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 2 cut(s) 502, 523
Tru1I TTAA 3 cut(s) 143, 546, 550
Tru9I TTAA 3 cut(s) 143, 546, 550
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 2 cut(s) 250, 427
TseI GCWGC 2 cut(s) 32, 245
Tsp45I GTSAC 2 cut(s) 250, 427
TspDTI ATGAA 6 cut(s) 17, 114, 261, 372, 423, 494
TspMI CCCGGG 1 cut(s) 85
TspRI CASTG 1 cut(s) 160
XapI RAATTY 3 cut(s) 4, 38, 278
XceI RCATGY 1 cut(s) 473
XmaI CCCGGG 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.