Rh1BG050200

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001233
Physical Location & Seq
Forward (+)
6917795 .. 6918790
996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG050200.1

Sequence Viewer

Length: 531 bp
ATGGTGTTCGATGAGGCTGATGTTAGACTTATTTCTGGACTGAACAGGAATTGCCCCACAAGCTTTGACAAAGGATCGCCAAAGGATCACAACTATCCTTCATTGGCATCTGTTGTTACACCAACGATACCGTTTAACATTAGCTTTCATAGAACAGTTAAAAATGTTGGCCTTTCAAACTCTACATACAAGGCCAAAATCTTGCCAAATTCTGAGGTTGACATCAAAGTGGTGCCTGAAGTTCTTTCCTTTAATTCACTAAATGAGGAGAAGACTTTCGATGTGATCATTGTCGGGAAAGCTTTGCAGGAGTCGGATAGTCCATATCCGGCCGGTTCAAAAACAATAACTACAGAGTCGGATCCACAGTCCATATCGGAAACAAAAACCACCCCAGATTTTAAATCCAAATATAAAAGGATCAAAATCAGAAAACCTACAGAAGACACTGGCATGGAATCCTATACCAAACACGTGAACCATCCTAGCCAAGGAAGAACGGTCAAAGCTTGTGGGAACCAGAATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

176

Amino Acids

19.46

Weight (kDa)

8.78

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 29 - 113 8.4e-17 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 232
AclWI GGATC 5 cut(s) 82, 93, 356, 369, 428
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 208
AcuI CTGAAG 1 cut(s) 258
AcvI CACGTG 1 cut(s) 475
AfiI CCNNNNNNNGG 1 cut(s) 491
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 2 cut(s) 177, 339
AluBI AGCT 4 cut(s) 63, 144, 302, 509
AluI AGCT 4 cut(s) 63, 144, 302, 509
AlwI GGATC 5 cut(s) 82, 93, 356, 369, 428
AoxI GGCC 3 cut(s) 169, 192, 330
ApoI RAATTY 1 cut(s) 208
Asp700I GAANNNNTTC 1 cut(s) 275
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 1 cut(s) 232
BbrPI CACGTG 1 cut(s) 475
BbsI GAAGAC 2 cut(s) 278, 450
BccI CCATC 1 cut(s) 489
BclI TGATCA 1 cut(s) 285
BfaI CTAG 1 cut(s) 486
BfmI CTRYAG 2 cut(s) 351, 438
BmiI GGNNCC 3 cut(s) 234, 363, 518
BmsI GCATC 1 cut(s) 116
BpiI GAAGAC 2 cut(s) 278, 450
BsaAI YACGTR 1 cut(s) 475
BsaBI GATNNNNATC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 490
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse118I RCCGGY 1 cut(s) 332
Bse1I ACTGG 1 cut(s) 454
Bse8I GATNNNNATC 1 cut(s) 425
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 1 cut(s) 481
BseJI GATNNNNATC 1 cut(s) 425
BseLI CCNNNNNNNGG 1 cut(s) 491
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 454
BseRI GAGGAG 1 cut(s) 281
BseX3I CGGCCG 1 cut(s) 330
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 3 cut(s) 171, 194, 332
BshNI GGYRCC 1 cut(s) 232
BsiEI CGRYCG 1 cut(s) 333
BsiSI CCGG 2 cut(s) 329, 333
BslI CCNNNNNNNGG 1 cut(s) 491
BsnI GGCC 3 cut(s) 171, 194, 332
Bsp143I GATC 5 cut(s) 74, 85, 285, 361, 420
BspANI GGCC 3 cut(s) 171, 194, 332
BspCNI CTCAG 1 cut(s) 205
BspLI GGNNCC 3 cut(s) 234, 363, 518
BspPI GGATC 5 cut(s) 82, 93, 356, 369, 428
BspT107I GGYRCC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 332
BsrI ACTGG 1 cut(s) 454
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 5 cut(s) 74, 85, 285, 361, 420
BssT1I CCWWGG 1 cut(s) 490
Bst4CI ACNGT 4 cut(s) 132, 157, 369, 502
BstBAI YACGTR 1 cut(s) 475
BstDEI CTNAG 1 cut(s) 213
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 1 cut(s) 481
BstKTI GATC 5 cut(s) 77, 88, 288, 364, 423
BstMBI GATC 5 cut(s) 74, 85, 285, 361, 420
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 1 cut(s) 60
BstSFI CTRYAG 2 cut(s) 351, 438
BstV2I GAAGAC 2 cut(s) 278, 450
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BstZI CGGCCG 1 cut(s) 330
BsuRI GGCC 3 cut(s) 171, 194, 332
BtsCI GGATG 1 cut(s) 481
BtsIMutI CAGTG 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 332
CspCI CAANNNNNGTGG 4 cut(s) 46, 81, 493, 528
CviAII CATG 1 cut(s) 454
CviJI RGCY 9 cut(s) 17, 63, 144, 171, 194, 302, 332, 489, 509
CviKI_1 RGCY 9 cut(s) 17, 63, 144, 171, 194, 302, 332, 489, 509
DdeI CTNAG 1 cut(s) 213
DpnI GATC 5 cut(s) 76, 87, 287, 363, 422
DpnII GATC 5 cut(s) 74, 85, 285, 361, 420
DraI TTTAAA 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 330
EagI CGGCCG 1 cut(s) 330
EclXI CGGCCG 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 490
Eco52I CGGCCG 1 cut(s) 330
Eco57I CTGAAG 1 cut(s) 258
Eco72I CACGTG 1 cut(s) 475
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 490
FaeI CATG 1 cut(s) 457
FaiI YATR 7 cut(s) 150, 187, 325, 374, 414, 455, 465
FatI CATG 1 cut(s) 453
FbaI TGATCA 1 cut(s) 285
FokI GGATG 1 cut(s) 468
FspBI CTAG 1 cut(s) 486
HaeIII GGCC 3 cut(s) 171, 194, 332
HapII CCGG 2 cut(s) 329, 333
Hin1II CATG 1 cut(s) 457
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 3 cut(s) 61, 300, 507
HinfI GANTC 3 cut(s) 311, 356, 458
HpaII CCGG 2 cut(s) 329, 333
Hpy166II GTNNAC 2 cut(s) 220, 478
Hpy188I TCNGA 5 cut(s) 214, 316, 361, 379, 431
Hpy188III TCNNGA 2 cut(s) 36, 295
Hpy8I GTNNAC 2 cut(s) 220, 478
HpyAV CCTTC 1 cut(s) 108
HpyCH4III ACNGT 4 cut(s) 132, 157, 369, 502
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 1 cut(s) 307
HpyF10VI GCNNNNNNNGC 1 cut(s) 60
HpyF3I CTNAG 1 cut(s) 213
HpySE526I ACGT 1 cut(s) 474
Hsp92II CATG 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 5 cut(s) 74, 85, 285, 361, 420
LpnPI CCDG 8 cut(s) 21, 31, 249, 293, 342, 346, 408, 435
LweI GCATC 1 cut(s) 116
MaeI CTAG 1 cut(s) 486
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 1 cut(s) 115
MalI GATC 5 cut(s) 76, 87, 287, 363, 422
MboI GATC 5 cut(s) 74, 85, 285, 361, 420
MboII GAAGA 3 cut(s) 283, 455, 507
MflI RGATCY 1 cut(s) 361
MluCI AATT 3 cut(s) 49, 208, 253
MlyI GAGTC 2 cut(s) 320, 365
MmeI TCCRAC 2 cut(s) 294, 339
MnlI CCTC 3 cut(s) 7, 208, 259
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 5 cut(s) 135, 159, 252, 402, 529
MslI CAYNNNNRTG 2 cut(s) 227, 452
MspI CCGG 2 cut(s) 329, 333
MwoI GCNNNNNNNGC 1 cut(s) 60
NdeII GATC 5 cut(s) 74, 85, 285, 361, 420
NlaIII CATG 1 cut(s) 457
NlaIV GGNNCC 3 cut(s) 234, 363, 518
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 458
PleI GAGTC 2 cut(s) 319, 364
PmaCI CACGTG 1 cut(s) 475
PmlI CACGTG 1 cut(s) 475
PpsI GAGTC 2 cut(s) 319, 364
Ppu21I YACGTR 1 cut(s) 475
PspCI CACGTG 1 cut(s) 475
PspN4I GGNNCC 3 cut(s) 234, 363, 518
PsuI RGATCY 1 cut(s) 361
RseI CAYNNNNRTG 2 cut(s) 227, 452
SaqAI TTAA 5 cut(s) 135, 159, 252, 402, 529
Sau3AI GATC 5 cut(s) 74, 85, 285, 361, 420
SchI GAGTC 2 cut(s) 320, 365
SetI ASST 7 cut(s) 65, 146, 219, 304, 439, 477, 511
SfaNI GCATC 1 cut(s) 116
SfcI CTRYAG 2 cut(s) 351, 438
SmiMI CAYNNNNRTG 2 cut(s) 227, 452
Sse9I AATT 3 cut(s) 49, 208, 253
SspMI CTAG 1 cut(s) 486
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 4 cut(s) 132, 157, 369, 502
TaiI ACGT 1 cut(s) 477
TaqI TCGA 2 cut(s) 9, 279
TasI AATT 3 cut(s) 49, 208, 253
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 5 cut(s) 135, 159, 252, 402, 529
Tru9I TTAA 5 cut(s) 135, 159, 252, 402, 529
TscAI CASTG 1 cut(s) 454
TspDTI ATGAA 2 cut(s) 90, 137
TspRI CASTG 1 cut(s) 454
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.