Rh1BG065700

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001233
Physical Location & Seq
Forward (+)
10006596 .. 10007132
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG065700.1

Sequence Viewer

Length: 537 bp
ATGAATGTTACTGACAATAACAGAGCCGCTGGTGAATTTGCATATGGATCCGGACATATCAATCCTGTTAAAGCTATAGACCCAGGGCTTGTGTATGAAGCTTCTAAGGAAGACTACATAAAGTTGCTCTGCTTGGTCTTAAACCAGACCGATGTTAGACTTATATCAGGAGATAACAACACTTGCTCTACAGGCTCCGACAAAGGATCTTTACAAGATCACAATTACCCTTCACTAGCAGCCGTTGTCGTACCAAACAAATCCTTTTCGTTAAATTTTCACAGAACAGTGAAGAATGTTGGCCTTGCAAACTCCACTTACAATGCTACAGTATTCGCCAACTCCACTGAAGTTGACATCAAAGTGGTGCCTCAAGTTCTTTCCTTCAAGTCATTGGATGAGGAGAAGACCTTTAATGTGACCGTTGCTGGAAAAGGTTTGTCCGATGAAGCACAATCACATGTGTCTGCATCGTTGGTGTGGTCTGATGGAATTCATAGCGTTAGAAGTCCAATTGTTATTTACAAAAAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

178

Amino Acids

19.22

Weight (kDa)

6.18

Isoelectric Point (pI)

24.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 73 - 174 1.2e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 367
AccIII TCCGGA 1 cut(s) 50
AciI CCGC 1 cut(s) 27
AclWI GGATC 3 cut(s) 42, 55, 214
AcsI RAATTY 3 cut(s) 35, 274, 492
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 1 cut(s) 252
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 1 cut(s) 388
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 2 cut(s) 74, 101
AluI AGCT 2 cut(s) 74, 101
AlwI GGATC 3 cut(s) 42, 55, 214
Aor13HI TCCGGA 1 cut(s) 50
AoxI GGCC 1 cut(s) 301
ApeKI GCWGC 1 cut(s) 239
ApoI RAATTY 3 cut(s) 35, 274, 492
AsuHPI GGTGA 1 cut(s) 44
BamHI GGATCC 1 cut(s) 47
BanI GGYRCC 1 cut(s) 367
BbsI GAAGAC 2 cut(s) 117, 413
BbvI GCAGC 1 cut(s) 251
BccI CCATC 1 cut(s) 482
BceAI ACGGC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 84
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 3 cut(s) 75, 189, 327
BisI GCNGC 2 cut(s) 27, 240
BlsI GCNGC 2 cut(s) 28, 241
Bme1390I CCNGG 1 cut(s) 84
BmiI GGNNCC 3 cut(s) 49, 196, 369
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 479
BpiI GAAGAC 2 cut(s) 117, 413
BpuEI CTTGAG 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 82, 83
BsaWI WCCGGW 1 cut(s) 50
BseAI TCCGGA 1 cut(s) 50
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 82, 83
BseGI GGATG 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 416
BseXI GCAGC 1 cut(s) 251
BshFI GGCC 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 367
BsiSI CCGG 1 cut(s) 51
BsnI GGCC 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 50
Bsp143I GATC 3 cut(s) 47, 206, 217
BspACI CCGC 1 cut(s) 27
BspANI GGCC 1 cut(s) 303
BspEI TCCGGA 1 cut(s) 50
BspLI GGNNCC 3 cut(s) 49, 196, 369
BspPI GGATC 3 cut(s) 42, 55, 214
BspT107I GGYRCC 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 82, 83
BssMI GATC 3 cut(s) 47, 206, 217
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 3 cut(s) 289, 331, 424
BstDEI CTNAG 1 cut(s) 105
BstF5I GGATG 1 cut(s) 403
BstKTI GATC 3 cut(s) 50, 209, 220
BstMBI GATC 3 cut(s) 47, 206, 217
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 82
BstSFI CTRYAG 3 cut(s) 75, 189, 327
BstV1I GCAGC 1 cut(s) 251
BstV2I GAAGAC 2 cut(s) 117, 413
BstX2I RGATCY 2 cut(s) 47, 206
BstYI RGATCY 2 cut(s) 47, 206
BsuRI GGCC 1 cut(s) 303
BtsCI GGATG 1 cut(s) 403
BtsIMutI CAGTG 2 cut(s) 294, 345
Csp6I GTAC 1 cut(s) 251
CspCI CAANNNNNGTGG 2 cut(s) 334, 369
CviAII CATG 1 cut(s) 461
CviJI RGCY 7 cut(s) 26, 74, 88, 101, 195, 242, 303
CviKI_1 RGCY 7 cut(s) 26, 74, 88, 101, 195, 242, 303
CviQI GTAC 1 cut(s) 251
DdeI CTNAG 1 cut(s) 105
DpnI GATC 3 cut(s) 49, 208, 219
DpnII GATC 3 cut(s) 47, 206, 217
Eco57I CTGAAG 1 cut(s) 369
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 82
FaeI CATG 1 cut(s) 464
FatI CATG 1 cut(s) 460
FauNDI CATATG 1 cut(s) 43
Fnu4HI GCNGC 2 cut(s) 27, 240
FokI GGATG 1 cut(s) 410
Fsp4HI GCNGC 2 cut(s) 27, 240
FspBI CTAG 1 cut(s) 236
GluI GCNGC 2 cut(s) 27, 240
HaeIII GGCC 1 cut(s) 303
HapII CCGG 1 cut(s) 51
Hin1II CATG 1 cut(s) 464
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 99
HpaII CCGG 1 cut(s) 51
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 3 cut(s) 199, 445, 487
Hpy188III TCNNGA 2 cut(s) 51, 168
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 240, 394
HpyCH4III ACNGT 3 cut(s) 289, 331, 424
HpyCH4V TGCA 3 cut(s) 41, 308, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 1 cut(s) 105
Hsp92II CATG 1 cut(s) 464
Kpn2I TCCGGA 1 cut(s) 50
Kzo9I GATC 3 cut(s) 47, 206, 217
LmnI GCTCC 1 cut(s) 200
LpnPI CCDG 9 cut(s) 15, 64, 69, 78, 96, 153, 158, 177, 414
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 1 cut(s) 479
MaeI CTAG 1 cut(s) 236
MaeIII GTNAC 2 cut(s) 7, 418
MalI GATC 3 cut(s) 49, 208, 219
MboI GATC 3 cut(s) 47, 206, 217
MboII GAAGA 3 cut(s) 122, 304, 418
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 2 cut(s) 47, 206
MluCI AATT 5 cut(s) 35, 223, 274, 492, 513
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 2 cut(s) 381, 394
MroI TCCGGA 1 cut(s) 50
MseI TTAA 4 cut(s) 69, 140, 272, 414
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 84
MunI CAATTG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 84
MwoI GCNNNNNNNGC 1 cut(s) 192
NdeI CATATG 1 cut(s) 43
NdeII GATC 3 cut(s) 47, 206, 217
NlaIII CATG 1 cut(s) 464
NlaIV GGNNCC 3 cut(s) 49, 196, 369
NmuCI GTSAC 1 cut(s) 418
NspI RCATGY 1 cut(s) 464
PasI CCCWGGG 1 cut(s) 83
PciI ACATGT 1 cut(s) 460
PkrI GCNGC 2 cut(s) 28, 241
PscI ACATGT 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 3 cut(s) 49, 196, 369
PsuI RGATCY 2 cut(s) 47, 206
RsaI GTAC 1 cut(s) 252
RsaNI GTAC 1 cut(s) 251
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 4 cut(s) 69, 140, 272, 414
SatI GCNGC 2 cut(s) 27, 240
Sau3AI GATC 3 cut(s) 47, 206, 217
ScrFI CCNGG 1 cut(s) 84
SetI ASST 4 cut(s) 76, 103, 413, 439
SfaNI GCATC 1 cut(s) 479
SfcI CTRYAG 3 cut(s) 75, 189, 327
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 372
SmoI CTYRAG 1 cut(s) 372
Sse9I AATT 5 cut(s) 35, 223, 274, 492, 513
SsiI CCGC 1 cut(s) 27
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 82
TaaI ACNGT 3 cut(s) 289, 331, 424
TaqII GACCGA 1 cut(s) 164
TasI AATT 5 cut(s) 35, 223, 274, 492, 513
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 4 cut(s) 69, 140, 272, 414
Tru9I TTAA 4 cut(s) 69, 140, 272, 414
TscAI CASTG 2 cut(s) 294, 352
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 1 cut(s) 239
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 4 cut(s) 17, 111, 462, 485
TspRI CASTG 2 cut(s) 294, 352
XapI RAATTY 3 cut(s) 35, 274, 492
XceI RCATGY 1 cut(s) 464
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.