Rroxscaffold_4G00326630

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001233
Physical Location & Seq
Forward (+)
58785670 .. 58790175
4506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326630.1

Sequence Viewer

Length: 738 bp
ATGACTCATCTTGTCATGCCAAAACTACTCTGTGATGTAGCTTACCAAGATGGAGCAATAGGTTCAATTTTAAATGCCGATTGGATTGATTTTTCCGATATTCTCACTAAACCTGCAACATTGTTGAGGGATCATGAGTATAATGCAATCAAGTTTTACATGAAGTCCATTATCAATGCTCGAGCAAACATATTAAAAAGTGAGGCCGAAAGAGATGATGATGTGCTCGTTATTGCTTCCTTCTCGCACGGTTATTATTTTGGCCGCCTTTTCACCTATTGCTTCAATTACGGAGAGGGTCCTCAAGACAAGAGGCGTGTAAAATATAGTATGCTCTCCGGAACCTCCATGTCTTGTCCGGACACGGCTGATGGAACTGCACATGTTAAAGCATTCCACCCCGACCGGTCTCCAGCAACAATCAAATCATCTCTTTTGACTACGGCTTGGCCTATGAATGTTACTAACAACTCTCCAGGGGAATTTGCTTATGGATCCGGACATATCAATCTCGTCCAAGCTATAAACCCGTGGCTGGTTTATGAAGCTTCTAAGGATGATTACATAAAACTCCTCTGTTCGGTATTGGGGGAGGGCGAGGTTAGACTTATATCGGGAGATAACAGCACTTGCCCTACACACTCGGACAAAGACGCGCCAAAGGATCACAATTACCCTTCACTAGCAATGTTGTTCCACCAAATAAAGCTTTTACCGTGCAATTTCACGAGTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

245

Amino Acids

27.26

Weight (kDa)

5.96

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 102 - 157 1.5e-07 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 121
AccII CGCG 1 cut(s) 656
AccIII TCCGGA 3 cut(s) 338, 358, 497
AciI CCGC 1 cut(s) 265
AclWI GGATC 4 cut(s) 138, 489, 502, 672
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 1 cut(s) 482
AfiI CCNNNNNNNGG 2 cut(s) 535, 580
AflIII ACRYGT 1 cut(s) 382
AgeI ACCGGT 1 cut(s) 405
AgsI TTSAA 2 cut(s) 66, 286
AjnI CCWGG 1 cut(s) 475
AluBI AGCT 4 cut(s) 41, 521, 548, 709
AluI AGCT 4 cut(s) 41, 521, 548, 709
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 4 cut(s) 138, 489, 502, 672
Ama87I CYCGRG 1 cut(s) 180
Aor13HI TCCGGA 3 cut(s) 338, 358, 497
AoxI GGCC 3 cut(s) 204, 262, 449
ApoI RAATTY 1 cut(s) 482
AsiGI ACCGGT 1 cut(s) 405
AspLEI GCGC 1 cut(s) 658
AspS9I GGNCC 1 cut(s) 299
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 299
BamHI GGATCC 1 cut(s) 494
BauI CACGAG 1 cut(s) 727
Bbv12I GWGCWC 1 cut(s) 228
BccI CCATC 2 cut(s) 44, 365
BceAI ACGGC 2 cut(s) 381, 459
BciT130I CCWGG 1 cut(s) 477
BcoDI GTCTC 1 cut(s) 414
BfaI CTAG 1 cut(s) 683
BfuAI ACCTGC 1 cut(s) 121
BisI GCNGC 1 cut(s) 265
BlsI GCNGC 1 cut(s) 266
Bme1390I CCNGG 1 cut(s) 477
Bme18I GGWCC 1 cut(s) 299
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 3 cut(s) 300, 343, 496
BmrFI CCNGG 1 cut(s) 477
BpmI CTGGAG 2 cut(s) 396, 459
BpuEI CTTGAG 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 414
BsaJI CCNNGG 2 cut(s) 476, 530
BsaWI WCCGGW 4 cut(s) 338, 358, 405, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 535, 580
Bse118I RCCGGY 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 693
BseAI TCCGGA 3 cut(s) 338, 358, 497
BseBI CCWGG 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 476, 530
BseGI GGATG 1 cut(s) 562
BseLI CCNNNNNNNGG 2 cut(s) 535, 580
BseMI GCAATG 1 cut(s) 693
BseRI GAGGAG 1 cut(s) 563
BsgI GTGCAG 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 656
Bsh1285I CGRYCG 1 cut(s) 406
BshFI GGCC 3 cut(s) 206, 264, 451
BshTI ACCGGT 1 cut(s) 405
BsiEI CGRYCG 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 4 cut(s) 339, 359, 406, 498
BslI CCNNNNNNNGG 2 cut(s) 535, 580
BsmAI GTCTC 1 cut(s) 414
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 3 cut(s) 206, 264, 451
Bso31I GGTCTC 1 cut(s) 414
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 1 cut(s) 228
Bsp13I TCCGGA 3 cut(s) 338, 358, 497
Bsp143I GATC 3 cut(s) 130, 494, 664
BspACI CCGC 1 cut(s) 265
BspANI GGCC 3 cut(s) 206, 264, 451
BspEI TCCGGA 3 cut(s) 338, 358, 497
BspFNI CGCG 1 cut(s) 656
BspHI TCATGA 1 cut(s) 133
BspLI GGNNCC 3 cut(s) 300, 343, 496
BspMI ACCTGC 1 cut(s) 121
BspPI GGATC 4 cut(s) 138, 489, 502, 672
BspTNI GGTCTC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 693
BsrFI RCCGGY 1 cut(s) 405
BssAI RCCGGY 1 cut(s) 405
BssECI CCNNGG 2 cut(s) 476, 530
BssMI GATC 3 cut(s) 130, 494, 664
BssSI CACGAG 1 cut(s) 727
Bst2BI CACGAG 1 cut(s) 727
Bst2UI CCWGG 1 cut(s) 477
Bst4CI ACNGT 2 cut(s) 251, 717
BstDEI CTNAG 1 cut(s) 552
BstDSI CCRYGG 1 cut(s) 530
BstF5I GGATG 1 cut(s) 562
BstFNI CGCG 1 cut(s) 656
BstHHI GCGC 1 cut(s) 658
BstKTI GATC 3 cut(s) 133, 497, 667
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 3 cut(s) 130, 494, 664
BstMCI CGRYCG 1 cut(s) 406
BstNI CCWGG 1 cut(s) 477
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 475
BstUI CGCG 1 cut(s) 656
BstX2I RGATCY 1 cut(s) 494
BstYI RGATCY 1 cut(s) 494
BsuRI GGCC 3 cut(s) 206, 264, 451
BtgI CCRYGG 1 cut(s) 530
BtsCI GGATG 1 cut(s) 562
BveI ACCTGC 1 cut(s) 121
CciI TCATGA 1 cut(s) 133
CfoI GCGC 1 cut(s) 658
Cfr10I RCCGGY 1 cut(s) 405
Cfr13I GGNCC 1 cut(s) 299
CseI GACGC 1 cut(s) 662
CspAI ACCGGT 1 cut(s) 405
CviAII CATG 5 cut(s) 16, 134, 160, 349, 383
DdeI CTNAG 1 cut(s) 552
DpnI GATC 3 cut(s) 132, 496, 666
DpnII GATC 3 cut(s) 130, 494, 664
DraI TTTAAA 1 cut(s) 72
EaeI YGGCCR 1 cut(s) 262
Eco31I GGTCTC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 475
FaeI CATG 5 cut(s) 19, 137, 163, 352, 386
FatI CATG 5 cut(s) 15, 133, 159, 348, 382
Fnu4HI GCNGC 1 cut(s) 265
FokI GGATG 1 cut(s) 569
Fsp4HI GCNGC 1 cut(s) 265
FspBI CTAG 1 cut(s) 683
GlaI GCGC 1 cut(s) 657
GluI GCNGC 1 cut(s) 265
GsuI CTGGAG 2 cut(s) 396, 459
HaeIII GGCC 3 cut(s) 206, 264, 451
HapII CCGG 4 cut(s) 339, 359, 406, 498
HgaI GACGC 1 cut(s) 662
HhaI GCGC 1 cut(s) 658
Hin1II CATG 5 cut(s) 19, 137, 163, 352, 386
Hin6I GCGC 1 cut(s) 656
HinP1I GCGC 1 cut(s) 656
HindIII AAGCTT 2 cut(s) 546, 707
HinfI GANTC 1 cut(s) 4
HpaII CCGG 4 cut(s) 339, 359, 406, 498
HphI GGTGA 1 cut(s) 265
Hpy188I TCNGA 2 cut(s) 97, 646
Hpy188III TCNNGA 7 cut(s) 134, 305, 339, 359, 498, 615, 727
HpyAV CCTTC 2 cut(s) 250, 687
HpyCH4III ACNGT 2 cut(s) 251, 717
HpyCH4V TGCA 4 cut(s) 116, 146, 380, 720
HpyF3I CTNAG 1 cut(s) 552
Hsp92II CATG 5 cut(s) 19, 137, 163, 352, 386
HspAI GCGC 1 cut(s) 656
Kpn2I TCCGGA 3 cut(s) 338, 358, 497
Kzo9I GATC 3 cut(s) 130, 494, 664
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 9 cut(s) 126, 352, 372, 419, 426, 462, 489, 511, 521
MaeI CTAG 1 cut(s) 683
MaeIII GTNAC 1 cut(s) 460
MalI GATC 3 cut(s) 132, 496, 666
MboI GATC 3 cut(s) 130, 494, 664
MflI RGATCY 1 cut(s) 494
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 5 cut(s) 66, 286, 482, 670, 721
MnlI CCTC 9 cut(s) 120, 196, 289, 306, 312, 355, 584, 586, 592
MroI TCCGGA 3 cut(s) 338, 358, 497
MseI TTAA 4 cut(s) 71, 194, 387, 736
MspI CCGG 4 cut(s) 339, 359, 406, 498
MspR9I CCNGG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 392
MvaI CCWGG 1 cut(s) 477
MvnI CGCG 1 cut(s) 656
NdeII GATC 3 cut(s) 130, 494, 664
NlaIII CATG 5 cut(s) 19, 137, 163, 352, 386
NlaIV GGNNCC 3 cut(s) 300, 343, 496
NspI RCATGY 1 cut(s) 386
PaeR7I CTCGAG 1 cut(s) 180
PagI TCATGA 1 cut(s) 133
PciI ACATGT 1 cut(s) 382
PctI GAATGC 1 cut(s) 392
PinAI ACCGGT 1 cut(s) 405
PkrI GCNGC 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 299
PscI ACATGT 1 cut(s) 382
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 475
PspGI CCWGG 1 cut(s) 475
PspN4I GGNNCC 3 cut(s) 300, 343, 496
PspPI GGNCC 1 cut(s) 299
PspPPI RGGWCCY 1 cut(s) 299
PspXI VCTCGAGB 1 cut(s) 180
PsuI RGATCY 1 cut(s) 494
SaqAI TTAA 4 cut(s) 71, 194, 387, 736
SatI GCNGC 1 cut(s) 265
Sau3AI GATC 3 cut(s) 130, 494, 664
Sau96I GGNCC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 477
SduI GDGCHC 1 cut(s) 228
SetI ASST 9 cut(s) 43, 64, 115, 278, 347, 523, 550, 603, 711
Sfr274I CTCGAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 299
SlaI CTCGAG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 180, 303
SmoI CTYRAG 2 cut(s) 180, 303
Sse9I AATT 5 cut(s) 66, 286, 482, 670, 721
SsiI CCGC 1 cut(s) 265
SspMI CTAG 1 cut(s) 683
StyD4I CCNGG 1 cut(s) 475
TaaI ACNGT 2 cut(s) 251, 717
TaqI TCGA 1 cut(s) 181
TasI AATT 5 cut(s) 66, 286, 482, 670, 721
TauI GCSGC 1 cut(s) 267
Tru1I TTAA 4 cut(s) 71, 194, 387, 736
Tru9I TTAA 4 cut(s) 71, 194, 387, 736
TspDTI ATGAA 3 cut(s) 176, 470, 558
TspGWI ACGGA 1 cut(s) 306
VpaK11BI GGWCC 1 cut(s) 299
XapI RAATTY 1 cut(s) 482
XceI RCATGY 1 cut(s) 386
XhoI CTCGAG 1 cut(s) 180
XspI CTAG 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.