Rh1DG059700

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001233
Physical Location & Seq
Reverse (-)
10323336 .. 10324798
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG059700.1

Sequence Viewer

Length: 570 bp
ATGACTACAGCTTGGCCTATGAATCATACAACCAACAGCTCATGCCCGGGTGAATTTGCATATGGATCCGGTCATATCAATCCTCTAAAAGCTATTGACCCAGGGCTTGTGTATGATGCTTCTGCAGAGGATTATATTAAGTTACTCTGCTTGGACATGGACGAGGCGAGAGTCAGACTTATATCAGGAGATAACAGCACTTGTCCTACAGCTTCTGAACAAGGATCGGCAAAGGATCTCAACTACCCTTCAATATCAGCTATTGTTACACCAATGACACGTTTTACGGTGAAAATACGTAGAAGAGTTACAAATGTTGGCCTTGTAAATTCCACTTACAAGGCCGAAATCATGCCAAACTCTCAAGTTGAAATCAAAGTGGAGCCTCAAGTTCTTTCCTTCAAGTCCGTGAACAAGGAGAAGACTTTTGATTTGACCATTGTTGGAAGAGGGTTGCCAGATGGATCACATATGTCTTCGTCCTTGGTTTGGTCCGACGGAACTCATAAGGTTAAAAGTCCAGTTCTAGTGCAAAGCTTATCAGCAGCAGCATCATCACGGTTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

189

Amino Acids

20.53

Weight (kDa)

7.74

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 79 - 177 8.5e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 5 cut(s) 60, 73, 232, 243, 472
AcsI RAATTY 2 cut(s) 53, 328
AfiI CCNNNNNNNGG 1 cut(s) 489
AflIII ACRYGT 1 cut(s) 278
AgsI TTSAA 3 cut(s) 252, 371, 403
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 6 cut(s) 11, 39, 92, 212, 260, 537
AluI AGCT 6 cut(s) 11, 39, 92, 212, 260, 537
AlwI GGATC 5 cut(s) 60, 73, 232, 243, 472
AlwNI CAGNNNCTG 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 46
AoxI GGCC 3 cut(s) 14, 319, 342
ApeKI GCWGC 2 cut(s) 545, 548
ApoI RAATTY 2 cut(s) 53, 328
ArsI GACNNNNNNTTYG 2 cut(s) 415, 447
AspS9I GGNCC 1 cut(s) 492
AsuC2I CCSGG 2 cut(s) 47, 48
AsuHPI GGTGA 2 cut(s) 62, 301
AvaI CYCGRG 1 cut(s) 46
AvaII GGWCC 1 cut(s) 492
BamHI GGATCC 1 cut(s) 65
BbsI GAAGAC 2 cut(s) 428, 468
BbvI GCAGC 2 cut(s) 557, 560
BccI CCATC 1 cut(s) 455
BciT130I CCWGG 1 cut(s) 102
BcnI CCSGG 2 cut(s) 47, 48
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 3 cut(s) 6, 123, 207
BisI GCNGC 2 cut(s) 546, 549
BlsI GCNGC 2 cut(s) 547, 550
Bme1390I CCNGG 3 cut(s) 47, 48, 102
Bme18I GGWCC 1 cut(s) 492
BmeT110I CYCGRG 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 492
BmiI GGNNCC 2 cut(s) 67, 384
BmrFI CCNGG 3 cut(s) 47, 48, 102
BmsI GCATC 2 cut(s) 106, 560
BpiI GAAGAC 2 cut(s) 428, 468
BpuEI CTTGAG 2 cut(s) 348, 372
BpuMI CCSGG 2 cut(s) 47, 48
BsaAI YACGTR 1 cut(s) 299
BsaJI CCNNGG 4 cut(s) 46, 100, 101, 483
BsaWI WCCGGW 1 cut(s) 68
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse1I ACTGG 1 cut(s) 521
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 4 cut(s) 46, 100, 101, 483
BseLI CCNNNNNNNGG 1 cut(s) 489
BseNI ACTGG 1 cut(s) 521
BseXI GCAGC 2 cut(s) 557, 560
BshFI GGCC 3 cut(s) 16, 321, 344
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 2 cut(s) 47, 69
BslI CCNNNNNNNGG 1 cut(s) 489
BsnI GGCC 3 cut(s) 16, 321, 344
BsoBI CYCGRG 1 cut(s) 46
Bsp143I GATC 4 cut(s) 65, 224, 235, 464
BspANI GGCC 3 cut(s) 16, 321, 344
BspLI GGNNCC 2 cut(s) 67, 384
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 5 cut(s) 60, 73, 232, 243, 472
BsrI ACTGG 1 cut(s) 521
BssECI CCNNGG 4 cut(s) 46, 100, 101, 483
BssMI GATC 4 cut(s) 65, 224, 235, 464
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 289, 561
Bst6I CTCTTC 2 cut(s) 298, 442
BstBAI YACGTR 1 cut(s) 299
BstKTI GATC 4 cut(s) 68, 227, 238, 467
BstMBI GATC 4 cut(s) 65, 224, 235, 464
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 45, 46, 100
BstSFI CTRYAG 3 cut(s) 6, 123, 207
BstSNI TACGTA 1 cut(s) 299
BstV1I GCAGC 2 cut(s) 557, 560
BstV2I GAAGAC 2 cut(s) 428, 468
BstX2I RGATCY 2 cut(s) 65, 235
BstYI RGATCY 2 cut(s) 65, 235
BsuRI GGCC 3 cut(s) 16, 321, 344
CaiI CAGNNNCTG 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 492
Cfr9I CCCGGG 1 cut(s) 46
CviAII CATG 3 cut(s) 42, 157, 352
DpnI GATC 4 cut(s) 67, 226, 237, 466
DpnII GATC 4 cut(s) 65, 224, 235, 464
Eam1104I CTCTTC 2 cut(s) 298, 442
EarI CTCTTC 2 cut(s) 298, 442
Eco105I TACGTA 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 3 cut(s) 45, 160, 355
FatI CATG 3 cut(s) 41, 156, 351
FauNDI CATATG 2 cut(s) 61, 471
Fnu4HI GCNGC 2 cut(s) 546, 549
Fsp4HI GCNGC 2 cut(s) 546, 549
FspBI CTAG 1 cut(s) 527
GluI GCNGC 2 cut(s) 546, 549
HaeIII GGCC 3 cut(s) 16, 321, 344
HapII CCGG 2 cut(s) 47, 69
Hin1II CATG 3 cut(s) 45, 160, 355
HindIII AAGCTT 1 cut(s) 535
HinfI GANTC 2 cut(s) 22, 171
HpaII CCGG 2 cut(s) 47, 69
HphI GGTGA 2 cut(s) 62, 301
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 3 cut(s) 176, 217, 496
Hpy188III TCNNGA 1 cut(s) 186
Hpy8I GTNNAC 1 cut(s) 412
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 2 cut(s) 258, 409
HpyCH4III ACNGT 2 cut(s) 289, 561
HpyCH4IV ACGT 2 cut(s) 280, 298
HpyCH4V TGCA 3 cut(s) 59, 125, 532
HpySE526I ACGT 2 cut(s) 280, 298
Hsp92II CATG 3 cut(s) 45, 160, 355
Kzo9I GATC 4 cut(s) 65, 224, 235, 464
LmnI GCTCC 1 cut(s) 382
LpnPI CCDG 7 cut(s) 60, 82, 87, 114, 171, 471, 534
Lsp1109I GCAGC 2 cut(s) 557, 560
LweI GCATC 2 cut(s) 106, 560
MaeI CTAG 1 cut(s) 527
MaeII ACGT 2 cut(s) 280, 298
MaeIII GTNAC 3 cut(s) 141, 265, 307
MalI GATC 4 cut(s) 67, 226, 237, 466
MboI GATC 4 cut(s) 65, 224, 235, 464
MboII GAAGA 4 cut(s) 315, 433, 459, 468
MflI RGATCY 2 cut(s) 65, 235
MluCI AATT 2 cut(s) 53, 328
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 2 cut(s) 424, 519
MnlI CCTC 5 cut(s) 93, 121, 157, 396, 443
MseI TTAA 3 cut(s) 138, 513, 568
MspI CCGG 2 cut(s) 47, 69
MspR9I CCNGG 3 cut(s) 47, 48, 102
MvaI CCWGG 1 cut(s) 102
NciI CCSGG 2 cut(s) 47, 48
NdeI CATATG 2 cut(s) 61, 471
NdeII GATC 4 cut(s) 65, 224, 235, 464
NlaIII CATG 3 cut(s) 45, 160, 355
NlaIV GGNNCC 2 cut(s) 67, 384
PasI CCCWGGG 1 cut(s) 101
PfeI GAWTC 1 cut(s) 22
PkrI GCNGC 2 cut(s) 547, 550
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 67, 384
PspPI GGNCC 1 cut(s) 492
PstI CTGCAG 1 cut(s) 127
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 2 cut(s) 65, 235
SaqAI TTAA 3 cut(s) 138, 513, 568
SatI GCNGC 2 cut(s) 546, 549
Sau3AI GATC 4 cut(s) 65, 224, 235, 464
Sau96I GGNCC 1 cut(s) 492
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 3 cut(s) 47, 48, 102
SetI ASST 9 cut(s) 13, 41, 94, 214, 262, 283, 301, 513, 539
SfaNI GCATC 2 cut(s) 106, 560
SfcI CTRYAG 3 cut(s) 6, 123, 207
SinI GGWCC 1 cut(s) 492
SmaI CCCGGG 1 cut(s) 48
SmlI CTYRAG 2 cut(s) 363, 387
SmoI CTYRAG 2 cut(s) 363, 387
SnaBI TACGTA 1 cut(s) 299
Sse9I AATT 2 cut(s) 53, 328
SspMI CTAG 1 cut(s) 527
StyD4I CCNGG 3 cut(s) 45, 46, 100
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 289, 561
TaiI ACGT 2 cut(s) 283, 301
TasI AATT 2 cut(s) 53, 328
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 3 cut(s) 138, 513, 568
Tru9I TTAA 3 cut(s) 138, 513, 568
TseI GCWGC 2 cut(s) 545, 548
TspDTI ATGAA 2 cut(s) 35, 554
TspGWI ACGGA 2 cut(s) 397, 513
TspMI CCCGGG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 492
XapI RAATTY 2 cut(s) 53, 328
XmaI CCCGGG 1 cut(s) 46
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.