Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000764

Physical Location & Seq

Genomic Coordinates Reverse (-)

40669940 .. 40675446

Gene Structure 5507 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1271000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3654 bp

ATGCTGACGAAATTCGAGACCAAGAGTAATCGGGTGAAAGGGCTGAGCTTTCACCCCAAGCGGCCATGGATCTTGGCCAGTCTCCACAGCGGTGTGATCCAGCTCTGGGATTACCGAATGGGCACTCTCATCGACCGTTTTGACGAGCACGATGGCCCTGTACGTGGCGTCCATTTCCACTCCTCTCAGCCGCTTTTCGTCTCCGGAGGGGATGATTACAAGATCAAGGTATGGAATTACAAGTTGCATAGGTGTTTGTTTACACTTCTTGGACACCTTGATTACATCCGAACTGTTCAATTCCATCATGAGTATCCGTGGATTGTTAGTGCGAGTGATGACCAGACAATTAGAATATGGAATTGGCAGTCCCGAACTTGTATTTCTGTGCTCACTGGACACAACCATTACGTCATGTGTGCCTTATTCCATCCAAAGGAGGATCTTGTTGTCTCAGCATCATTGGATCAAACTGTTCGTGTTTGGGATATTGGTGCCCTCAGGAAAAAGACGGTTGCCCCAGCAGATGACATCATGCGCTTGAGTCAGATGAATGCAGATTTCTTTGGTGGAGTTGATGCTGTTGTGAAGTATGTTTTGGAAGGTCATGATCGGGGTGTGAATTGGGCATCATTTCATCCTACACTCCCTCTGATCGTCTCTGGAGCAGACGATCGACAAGTGAAACTTTGGCGGATGAATGATACAAAGGCTTGGGAAGTGGACACTCTAAGGGGGCACATGAACAATGTATCCTGTGTTCTATTCCATTCAAGGCAGGATATTATTGTGTCCAATTCAGAGGATAGAAGTATCCGAGTCTGGGATGCAACAAAACGAACTGGTCTTCAGACATTTCGTAGGGAGCATGATAGGTTCTGGATTCTTTCAGCACATCCTGAAATGAACCTCTTGGCTGCTGGGCATGATAGTGGCATGATTGTCTTTAAATTGGAGAGGGAGCGTCCTGCTTTTTCTGTTAGCGGTGATTCAATGTACTATGTTAAAGATCGTTTTCTCCGCTTCTATGAGTTCTCCACCCAGAGAGACAGTCAGGTTATCCCAATTCGAAGGCCTGGTTCTTCCAACTTGAATCAAGGGGCAAAAACACTATCTTACAGCCCTACCGAAAATGCTGTTTTGATAGGTTCAGATACGGAAGGGGGTTCTTATGAACTATACATTATACCCAAAGATAGCTTTGGTCGGGGTGATACTATGCAGGAGGCAAAAAGAGGAATCGGAGGCCCAGCTGTGTTCGTGGCTCGCAATAGGTTTGCAGTGCTTGAAAAGAGCACCAACCAAGTTATAGTTAAAAATCTGAAAAATGAGATTGTCAAGAAGAGTGCCCTACCTATTGTTGCTGATGCAATATTTTATGCTGGAACTGGAAACTTGCTCTGCAGGGCAGAGGACAGAGTCATTATTTTTGACCTGCAGCAGAGAATTATTGTTGGAGAGCTTCAAACTCCTTTTGTAAGGTATATTGTTTGGTCAAATGACATGGAGAGCGTTGCTTTACTTAGCAAACATTCAATTGTGATAGCCAATAAGAAGCTTGTGCATCAGTGCACTCTCCATGAGACAATTCGTGTAAAGAGTGGAGCTTGGGATGACAATGGCGTCTTCATATATACAACCTTGAACCACATCAAATACTGCCTTCCTAATGGGGATAGTGGAATCATCAGGACCCTTGACATTCCAGTGTACATAACAAAAGTGTATGGAAACACCATACATTGCTTGGATCGAGATGGGAAGAACTGTGCCATTGTTGTTGATGCAACAGAATATGTTTTCAAGTTATCCTTACTGAAGAAAAGGTATGACCAGGTTATGAGCATGATTAGGAGTTCTGAGCTCTGTGGCCAGGCCATGATTGCATATCTACAGCAGAAAGGTTTCCCTGAAGTTGCTCTTCATTTTGTGAAGGATGAGAGGACTCGCTTCAACTTGGCACTAGGGAGTGGGAACATCCAGATTGCTGTTGCTTCTGCCAAGGAAATTGATGAGAAAGATCACTGGTATCGATTGGGAGTGGAGGCCCTACGTCAGGGTAATGCAGGCATTGTAGAATATGCGTATCAGAGGACAAAGAATTTTGAGAGGCTATCATTCCTATATCTTGTGACTGGAAACTTGGATAAATTGTCTAAAATGCTGAAAATTGCTGAAGTCAAGAATGATGTGATGGGTCAGTTCCACAATGCTTTGTATTTGGGTGATATCAGAGAACGTGTTAAGATCTTGGAGAATGCAGGTCATTTACCTCTTGCTTATAGCACAGCTGTAATCCATGGGCTCCATGATATTGCTGAACGTTTGGCAGCTGAACTGGGAGATAATGTTCCTGTTTTACCCAAGGGGAAATCTCCCTCTCTTTTGATACCTCCTACGCCAATCATCTGCGGTGGAGATTGGCCCTTGTTGAGGGTCATGAGAGGAATATTTGAGGGTGGTCTTGATAATGTGGGCAAGAATGTTGAGGAAGAGTATGAAGAGGCTACAGATGCTGATTGGGGAGAGGATTTGGATATTGTTGATGTGGACAAGATTCCAAATGGAGACATCAGTGCAGTGCTGGATGATGAGGAAGCACACGAGGAAAATGAGGAGGAAGGATGGGATCTTGAAGATCTTGAACTTCCACCTGAGATTGATACTCCAAAAACTGCCAGCAATGCCCGTTCTTCTGTGTTTGTTGCCCCAACTCCAGGTATGCCTGTAAGTCAAATCTGGTCCCAGAAATCATCTCTGGCTGCCGAACATGCTGCAGCTGGGAATTTCGATATTGCAATGCGTTTGTTGAGCAGGCAGCTGGGTATAAAGAACTTTGATCCCTTAAGACAATTATTTCTCGATTTACACATGGGCAGTCATACGTATCTACGTGCCTTCTCGACAGCTCCTGTGCTTACCGTGGCCATTGAGAGGGGATGGAGTGAATCAGCTACTCCTAATGTTAGGGGTCCTCCTGCTCTTGTATTTAAATTCTCTGAGCTAGAAGAGAAGCTTAAGGTGGGTTACAAGGCCACAACCCAAGGGAAATTTGCTGATGCTTTACGTATTTTCCTTGGTATTCTGCATACTATTCCTCTGATTGTAGTTGATTCAAGGAGAGAGGTTGATGAAGTAAAAGAATTAATAATCATTGTAAAGGAGTATGTTCTGGGTTTGAAGATGGAGATTAAGAGGAAGGAACTTAAAGGCGATCCGGTTCGCCAACAGGAGCTAGCAGCTTACTTCACCCACTGCAACCTTCAGATCCCTCACTTGAGGCTAGCATTACTAAATGCCATGAGTGTTTGCTATAAGGCTGGTAATCTCAACACGGCAGCTAACTTTGCCAGGCGACTTTTGGAGACCAATCCCAGCGTTGAAAACCATGCAAAGACTGCAAGACAAATCCTGCAAGCTGCCGAAAAGAACATGACTGATGCAACCCAGCTAAACTATGATTTCAGAAACCCATTTGTGGTTTGTGGGGGAACATACGTGCCTATTTACCGTGGACAGAAGGATGTGTCATGCCCGTACTGCAGCTCTCGGTTTGTTCCTGACCATGAAGGGAAGATCTGTTCTGTTTGTGATCTTGCAGTGGTTGGATCAGATGCATCTGGTTTGCTCTGCTCTCCTACCCAGATAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COPI_C Coatomer_WDAD WD40

Protein Analysis

1218

Amino Acids

136.99

Weight (kDa)

6.59

Isoelectric Point (pI)

30.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR36-Utp21_1st	PF25171	2 - 79	3.3e-08	WDR36/Utp21 first beta-propeller
Beta-prop_WDR5	PF25175	3 - 90	1.8e-10	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	4 - 162	1.4e-15	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	4 - 164	4e-32	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	6 - 127	1.4e-17	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	10 - 83	2.6e-06	WDR3 second beta-propeller domain
WDR55	PF24796	10 - 166	2.5e-12	WDR55
Beta-prop_EML_2	PF23414	13 - 140	5.7e-08	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	26 - 122	2.6e-07	G protein beta WD-40 repeat protein
WD40	PF00400	42 - 79	2.9e-06	WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd	PF25168	49 - 165	5.3e-16	WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	50 - 166	7.1e-08	CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st	PF24817	51 - 130	1e-09	WDHD1 first WD40 domain
WD40_Prp19	PF24814	61 - 167	8.4e-20	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	67 - 166	8.4e-28	WDR5 beta-propeller domain
WD40	PF00400	84 - 121	3e-08	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	88 - 163	1.6e-08	WDR3 second beta-propeller domain
Beta-prop_EIPR1	PF23609	91 - 162	1.9e-06	EIPR1 beta-propeller
WD40_WDHD1_1st	PF24817	96 - 166	3.6e-12	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	108 - 166	2.5e-12	THOC3 beta-propeller domain
WD40	PF00400	126 - 163	6.4e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	185 - 303	3.7e-06	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	195 - 314	4e-17	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	196 - 317	3.5e-18	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	196 - 308	2.3e-20	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	197 - 292	7.3e-07	TEP-1 second beta-propeller
WD40	PF00400	198 - 231	3e-06	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	202 - 311	4.9e-09	WDR3 second beta-propeller domain
WDR55	PF24796	207 - 315	9.1e-09	WDR55
Beta-prop_THOC3	PF25174	208 - 316	4.2e-14	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	211 - 286	6.6e-09	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	212 - 319	1.7e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	223 - 320	5.8e-07	CDC20/Fizzy WD40 domain
WD40	PF00400	241 - 276	8.4e-07	WD domain, G-beta repeat
B-prop_COPA_B_2nd	PF04053	340 - 584	8.5e-67	COPA/B second beta-propeller
TPR_COPA_B	PF23953	626 - 761	4.6e-18	COPA/B TPR domain
COPI_C	PF06957	815 - 1217	9.5e-173	Coatomer (COPI) alpha subunit C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62020 AT2G21390
fragaria_vesca	FvH4_3g10280 FvH4_5g12340 FvH4_5g12340 FvH4_5g12391 FvH4_5g12420
malus_domestica	MD05G1271000.v1.1 MD06G1214600.v1.1 MD10G1248600.v1.1 MD10G1248700.v1.1 MD10G1248800.v1.1 MD10G1249800.v1.1 MD10G1249900.v1.1 MD10G1250100.v1.1 MD10G1250200.v1.1 MD10G1250400.v1.1 MD10G1250600.v1.1 MD10G1250700.v1.1 MD10G1251000.v1.1 MD10G1251300.v1.1 MD10G1251600.v1.1 MD14G1225500.v1.1
prunus_persica	Prupe.4G091800_v2.0.a1 Prupe.4G091800_v2.0.a1 Prupe.5G220000_v2.0.a1 Prupe.5G220000_v2.0.a1
pyrus_communis	pycom05g25340 pycom06g19120 pycom10g20750 pycom14g18670
rosa_chinensis	RchiOBHm_Chr5g0016541 RchiOBHm_Chr7g0185121 RchiOBHm_Chr7g0185201
rosa_laevigata	RLG00000000634 RLG00000000671 RLG00000001958 RLG00000004933 RLG00000032256
rosa_multiflora	Rmu_co8505923.1_g000001 Rmu_co8507327.1_g000001 Rmu_sc0001235.1_g000018 Rmu_sc0004765.1_g000006 Rmu_sc0007192.1_g000011 Rmu_sc0019416.1_g000003 Rmu_ssc0000398.1_g000013
rosa_roxburghii	Rroxscaffold_1G00060340 Rroxscaffold_3G00269160 Rroxscaffold_3G00269190
rosa_rugosa	Rorug05G0032700 Rorug06G0466700 Rorug06G0466700 Rorug06G0466800 Rorug07G0188600
rosa_samantha	Rh4BG061900 Rh4CG068200 Rh4DG290700 Rh5AG127000 Rh5AG459900 Rh5BG125900 Rh5CG098400 Rh5CG138600 Rh5DG126100 Rh7AG076100 Rh7BG071200 Rh7CG072400 Rh7DG071500 Rh7DG071900
rosa_wichuraiana	Rw5G011000 Rw7G005980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1622
Acc36I	ACCTGC	2 cut(s)	1443, 2252
AccB1I	GGYRCC	1 cut(s)	494
AccIII	TCCGGA	1 cut(s)	203
AciI	CCGC	7 cut(s)	61, 90, 191, 694, 984, 1021, 2412
AclI	AACGTT	1 cut(s)	2323
AcoI	YGGCCR	4 cut(s)	62, 75, 1870, 2925
AcsI	RAATTY	5 cut(s)	11, 2101, 2785, 2993, 3050
AcuI	CTGAAG	5 cut(s)	833, 1838, 1932, 2196, 3248
AcyI	GRCGYC	2 cut(s)	168, 1623
AfaI	GTAC	4 cut(s)	162, 998, 1712, 3539
AfiI	CCNNNNNNNGG	9 cut(s)	106, 164, 436, 823, 2056, 2433, 2717, 2934, 3478
AflII	CTTAAG	2 cut(s)	2845, 3017
AflIII	ACRYGT	1 cut(s)	2239
AjnI	CCWGG	5 cut(s)	1075, 1833, 1872, 2716, 3350
AjuI	GAANNNNNNNTTGG	4 cut(s)	346, 378, 581, 613
AleI	CACNNNNGTG	1 cut(s)	90
AloI	GAACNNNNNNTCC	6 cut(s)	737, 769, 2334, 2366, 3565, 3597
Alw21I	GWGCWC	5 cut(s)	150, 393, 1298, 1574, 1866
Alw26I	GTCTC	9 cut(s)	11, 86, 205, 457, 664, 1041, 1577, 2562, 3359
Alw44I	GTGCAC	1 cut(s)	1570
AlwNI	CAGNNNCTG	2 cut(s)	2516, 2912
Aor13HI	TCCGGA	1 cut(s)	203
ApaLI	GTGCAC	1 cut(s)	1570
ApoI	RAATTY	5 cut(s)	11, 2101, 2785, 2993, 3050
AseI	ATTAAT	1 cut(s)	3146
Asp700I	GAANNNNTTC	1 cut(s)	1904
AspLEI	GCGC	1 cut(s)	540
AspS9I	GGNCC	7 cut(s)	155, 1247, 1692, 2047, 2424, 2742, 2972
AsuHPI	GGTGA	6 cut(s)	44, 46, 998, 1223, 2237, 3241
AsuII	TTCGAA	1 cut(s)	1069
AsuNHI	GCTAGC	2 cut(s)	3235, 3283
AvaII	GGWCC	3 cut(s)	1692, 2742, 2972
AxyI	CCTNAGG	1 cut(s)	500
BaeGI	GKGCMC	5 cut(s)	125, 499, 741, 1351, 1574
BalI	TGGCCA	3 cut(s)	77, 1872, 2927
BanI	GGYRCC	1 cut(s)	494
BanII	GRGCYC	2 cut(s)	1866, 2307
BauI	CACGAG	1 cut(s)	2603
BbsI	GAAGAC	2 cut(s)	839, 1618
Bbv12I	GWGCWC	5 cut(s)	150, 393, 1298, 1574, 1866
BccI	CCATC	8 cut(s)	146, 312, 438, 1751, 2188, 2619, 2934, 3178
BceAI	ACGGC	1 cut(s)	3351
BcgI	CGANNNNNNTGC	4 cut(s)	112, 146, 2756, 2790
BciT130I	CCWGG	5 cut(s)	1077, 1835, 1874, 2718, 3352
BciVI	GTATCC	3 cut(s)	324, 763, 824
BcoDI	GTCTC	9 cut(s)	11, 86, 205, 457, 664, 1041, 1577, 2562, 3359
BfaI	CTAG	4 cut(s)	1964, 3005, 3236, 3284
BfmI	CTRYAG	6 cut(s)	1402, 1436, 1892, 2508, 2775, 3541
BfrI	CTTAAG	2 cut(s)	2845, 3017
BfuAI	ACCTGC	2 cut(s)	1443, 2252
BfuI	GTATCC	3 cut(s)	324, 763, 824
BglII	AGATCT	3 cut(s)	2247, 2638, 3576
BlpI	GCTNAGC	1 cut(s)	44
Bme1390I	CCNGG	5 cut(s)	1077, 1835, 1874, 2718, 3352
Bme18I	GGWCC	3 cut(s)	1692, 2742, 2972
BmgT120I	GGNCC	7 cut(s)	155, 1247, 1692, 2047, 2424, 2742, 2972
BmiI	GGNNCC	5 cut(s)	496, 1694, 2306, 2744, 2973
BmrFI	CCNGG	5 cut(s)	1077, 1835, 1874, 2718, 3352
BmrI	ACTGGG	1 cut(s)	2348
BmtI	GCTAGC	2 cut(s)	3239, 3287
BmuI	ACTGGG	1 cut(s)	2348
BpiI	GAAGAC	2 cut(s)	839, 1618
BpmI	CTGGAG	2 cut(s)	684, 2700
Bpu1102I	GCTNAGC	1 cut(s)	44
Bpu14I	TTCGAA	1 cut(s)	1069
BpuEI	CTTGAG	2 cut(s)	562, 3298
Bsa29I	ATCGAT	1 cut(s)	2032
BsaAI	YACGTR	5 cut(s)	164, 2886, 2894, 3068, 3499
BsaHI	GRCGYC	2 cut(s)	168, 1623
BsaI	GGTCTC	2 cut(s)	11, 3359
BsaJI	CCNNGG	9 cut(s)	65, 317, 2001, 2299, 2364, 2922, 3043, 3076, 3511
BsaWI	WCCGGW	2 cut(s)	203, 3217
BsaXI	ACNNNNNCTCC	2 cut(s)	2334, 2364
Bsc4I	CCNNNNNNNGG	9 cut(s)	106, 164, 436, 823, 2056, 2433, 2717, 2934, 3478
Bse1I	ACTGG	8 cut(s)	78, 400, 847, 1393, 1706, 2030, 2140, 2343
Bse21I	CCTNAGG	1 cut(s)	500
Bse3DI	GCAATG	3 cut(s)	1741, 2689, 2805
BseAI	TCCGGA	1 cut(s)	203
BseBI	CCWGG	5 cut(s)	1077, 1835, 1874, 2718, 3352
BseCI	ATCGAT	1 cut(s)	2032
BseDI	CCNNGG	9 cut(s)	65, 317, 2001, 2299, 2364, 2922, 3043, 3076, 3511
BseLI	CCNNNNNNNGG	9 cut(s)	106, 164, 436, 823, 2056, 2433, 2717, 2934, 3478
BseMI	GCAATG	3 cut(s)	1741, 2689, 2805
BseMII	CTCAG	7 cut(s)	35, 200, 468, 514, 1851, 2646, 2991
BseNI	ACTGG	8 cut(s)	78, 400, 847, 1393, 1706, 2030, 2140, 2343
BseRI	GAGGAG	2 cut(s)	172, 2630
BseSI	GKGCMC	5 cut(s)	125, 499, 741, 1351, 1574
BseYI	CCCAGC	7 cut(s)	520, 920, 1249, 2780, 2821, 3374, 3447
BsgI	GTGCAG	1 cut(s)	2598
Bsh1285I	CGRYCG	2 cut(s)	136, 676
BshNI	GGYRCC	1 cut(s)	494
BshVI	ATCGAT	1 cut(s)	2032
BsiEI	CGRYCG	2 cut(s)	136, 676
BsiHKAI	GWGCWC	5 cut(s)	150, 393, 1298, 1574, 1866
BsiSI	CCGG	2 cut(s)	204, 3218
BslFI	GGGAC	2 cut(s)	355, 2728
BslI	CCNNNNNNNGG	9 cut(s)	106, 164, 436, 823, 2056, 2433, 2717, 2934, 3478
BsmAI	GTCTC	9 cut(s)	11, 86, 205, 457, 664, 1041, 1577, 2562, 3359
BsmBI	CGTCTC	2 cut(s)	205, 664
BsmFI	GGGAC	2 cut(s)	355, 2728
BsmI	GAATGC	2 cut(s)	559, 2263
Bso31I	GGTCTC	2 cut(s)	11, 3359
Bsp119I	TTCGAA	1 cut(s)	1069
Bsp13I	TCCGGA	1 cut(s)	203
Bsp1407I	TGTACA	1 cut(s)	1710
Bsp1720I	GCTNAGC	1 cut(s)	44
Bsp19I	CCATGG	2 cut(s)	65, 2299
BspACI	CCGC	7 cut(s)	61, 90, 191, 694, 984, 1021, 2412
BspCNI	CTCAG	7 cut(s)	36, 199, 467, 513, 1852, 2647, 2992
BspDI	ATCGAT	1 cut(s)	2032
BspEI	TCCGGA	1 cut(s)	203
BspHI	TCATGA	3 cut(s)	307, 607, 2439
BspLI	GGNNCC	5 cut(s)	496, 1694, 2306, 2744, 2973
BspMAI	CTGCAG	4 cut(s)	1406, 1440, 2779, 3545
BspMI	ACCTGC	2 cut(s)	1443, 2252
BspOI	GCTAGC	2 cut(s)	3239, 3287
BspQI	GCTCTTC	1 cut(s)	1926
BspT104I	TTCGAA	1 cut(s)	1069
BspT107I	GGYRCC	1 cut(s)	494
BspTI	CTTAAG	2 cut(s)	2845, 3017
BspTNI	GGTCTC	2 cut(s)	11, 3359
BsrDI	GCAATG	3 cut(s)	1741, 2689, 2805
BsrGI	TGTACA	1 cut(s)	1710
BsrI	ACTGG	8 cut(s)	78, 400, 847, 1393, 1706, 2030, 2140, 2343
BssECI	CCNNGG	9 cut(s)	65, 317, 2001, 2299, 2364, 2922, 3043, 3076, 3511
BssNI	GRCGYC	2 cut(s)	168, 1623
BssSI	CACGAG	1 cut(s)	2603
BssT1I	CCWWGG	6 cut(s)	65, 2001, 2299, 2364, 3043, 3076
Bst2BI	CACGAG	1 cut(s)	2603
Bst2UI	CCWGG	5 cut(s)	1077, 1835, 1874, 2718, 3352
Bst4CI	ACNGT	8 cut(s)	137, 295, 475, 514, 1052, 1769, 2923, 3512
Bst6I	CTCTTC	5 cut(s)	1337, 1926, 2487, 2496, 3003
BstACI	GRCGYC	2 cut(s)	168, 1623
BstAFI	CTTAAG	2 cut(s)	2845, 3017
BstAPI	GCANNNNNTGC	1 cut(s)	3398
BstAUI	TGTACA	1 cut(s)	1710
BstBAI	YACGTR	5 cut(s)	164, 2886, 2894, 3068, 3499
BstBI	TTCGAA	1 cut(s)	1069
BstC8I	GCNNGC	7 cut(s)	1267, 2068, 2680, 2816, 3237, 3285, 3417
BstDEI	CTNAG	9 cut(s)	44, 186, 454, 500, 731, 1523, 1860, 2655, 3000
BstDSI	CCRYGG	5 cut(s)	65, 317, 2299, 2922, 3511
BstENI	CCTNNNNNAGG	2 cut(s)	2054, 2431
BstHHI	GCGC	1 cut(s)	540
BstMAI	GTCTC	9 cut(s)	11, 86, 205, 457, 664, 1041, 1577, 2562, 3359
BstMCI	CGRYCG	2 cut(s)	136, 676
BstMWI	GCNNNNNNNGC	7 cut(s)	1883, 2513, 2684, 2771, 3347, 3398, 3540
BstNI	CCWGG	5 cut(s)	1077, 1835, 1874, 2718, 3352
BstNSI	RCATGY	1 cut(s)	2774
BstSCI	CCNGG	5 cut(s)	1075, 1833, 1872, 2716, 3350
BstSFI	CTRYAG	6 cut(s)	1402, 1436, 1892, 2508, 2775, 3541
BstSLI	GKGCMC	5 cut(s)	125, 499, 741, 1351, 1574
BstSNI	TACGTA	2 cut(s)	2886, 3068
BstV2I	GAAGAC	2 cut(s)	839, 1618
BstX2I	RGATCY	7 cut(s)	69, 442, 2247, 2629, 2638, 3267, 3576
BstYI	RGATCY	7 cut(s)	69, 442, 2247, 2629, 2638, 3267, 3576
Bsu15I	ATCGAT	1 cut(s)	2032
Bsu36I	CCTNAGG	1 cut(s)	500
BsuI	GTATCC	3 cut(s)	324, 763, 824
BsuTUI	ATCGAT	1 cut(s)	2032
BtgI	CCRYGG	5 cut(s)	65, 317, 2299, 2922, 3511
BtsI	GCAGTG	4 cut(s)	1287, 2586, 3253, 3606
BtsIMutI	CAGTG	9 cut(s)	393, 1287, 1574, 1713, 2023, 2581, 2586, 3253, 3606
BveI	ACCTGC	2 cut(s)	1443, 2252
Cac8I	GCNNGC	7 cut(s)	1267, 2068, 2680, 2816, 3237, 3285, 3417
CaiI	CAGNNNCTG	2 cut(s)	2516, 2912
CciI	TCATGA	3 cut(s)	307, 607, 2439
CfoI	GCGC	1 cut(s)	540
Cfr13I	GGNCC	7 cut(s)	155, 1247, 1692, 2047, 2424, 2742, 2972
ClaI	ATCGAT	1 cut(s)	2032
CseI	GACGC	3 cut(s)	157, 953, 1612
CsiI	ACCWGGT	1 cut(s)	1833
Csp6I	GTAC	4 cut(s)	161, 997, 1711, 3538
CviQI	GTAC	4 cut(s)	161, 997, 1711, 3538
DdeI	CTNAG	9 cut(s)	44, 186, 454, 500, 731, 1523, 1860, 2655, 3000
DraI	TTTAAA	2 cut(s)	949, 2992
DrdI	GACNNNNNNGTC	1 cut(s)	1622
DseDI	GACNNNNNNGTC	1 cut(s)	1622
EaeI	YGGCCR	4 cut(s)	62, 75, 1870, 2925
Eam1104I	CTCTTC	5 cut(s)	1337, 1926, 2487, 2496, 3003
EarI	CTCTTC	5 cut(s)	1337, 1926, 2487, 2496, 3003
EciI	GGCGGA	1 cut(s)	709
Ecl136II	GAGCTC	1 cut(s)	1864
Eco105I	TACGTA	2 cut(s)	2886, 3068
Eco130I	CCWWGG	6 cut(s)	65, 2001, 2299, 2364, 3043, 3076
Eco147I	AGGCCT	1 cut(s)	1075
Eco24I	GRGCYC	2 cut(s)	1866, 2307
Eco31I	GGTCTC	2 cut(s)	11, 3359
Eco32I	GATATC	1 cut(s)	2230
Eco47I	GGWCC	3 cut(s)	1692, 2742, 2972
Eco53kI	GAGCTC	1 cut(s)	1864
Eco57I	CTGAAG	5 cut(s)	833, 1838, 1932, 2196, 3248
Eco81I	CCTNAGG	1 cut(s)	500
EcoICRI	GAGCTC	1 cut(s)	1864
EcoNI	CCTNNNNNAGG	2 cut(s)	2054, 2431
EcoO109I	RGGNCCY	3 cut(s)	1692, 2047, 2972
EcoRII	CCWGG	5 cut(s)	1075, 1833, 1872, 2716, 3350
EcoRV	GATATC	1 cut(s)	2230
EcoT14I	CCWWGG	6 cut(s)	65, 2001, 2299, 2364, 3043, 3076
EcoT22I	ATGCAT	1 cut(s)	3619
EcoT38I	GRGCYC	2 cut(s)	1866, 2307
ErhI	CCWWGG	6 cut(s)	65, 2001, 2299, 2364, 3043, 3076
Esp3I	CGTCTC	2 cut(s)	205, 664
FalI	AAGNNNNNCTT	8 cut(s)	672, 704, 1796, 1828, 1905, 1937, 3000, 3032
FaqI	GGGAC	2 cut(s)	355, 2728
FriOI	GRGCYC	2 cut(s)	1866, 2307
FspBI	CTAG	4 cut(s)	1964, 3005, 3236, 3284
GlaI	GCGC	1 cut(s)	539
GsaI	CCCAGC	7 cut(s)	524, 924, 1253, 2784, 2825, 3378, 3451
GsuI	CTGGAG	2 cut(s)	684, 2700
HapII	CCGG	2 cut(s)	204, 3218
HgaI	GACGC	3 cut(s)	157, 953, 1612
HhaI	GCGC	1 cut(s)	540
Hin1I	GRCGYC	2 cut(s)	168, 1623
Hin6I	GCGC	1 cut(s)	538
HinP1I	GCGC	1 cut(s)	538
HindIII	AAGCTT	2 cut(s)	1556, 3014
HpaII	CCGG	2 cut(s)	204, 3218
HphI	GGTGA	6 cut(s)	44, 46, 998, 1223, 2237, 3241
Hpy166II	GTNNAC	6 cut(s)	261, 724, 1572, 1711, 2551, 3515
Hpy8I	GTNNAC	6 cut(s)	261, 724, 1572, 1711, 2551, 3515
HpyCH4III	ACNGT	8 cut(s)	137, 295, 475, 514, 1052, 1769, 2923, 3512
HpyCH4IV	ACGT	9 cut(s)	163, 411, 2053, 2239, 2323, 2885, 2893, 3067, 3498
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1883, 2513, 2684, 2771, 3347, 3398, 3540
HpyF3I	CTNAG	9 cut(s)	44, 186, 454, 500, 731, 1523, 1860, 2655, 3000
HpySE526I	ACGT	9 cut(s)	163, 411, 2053, 2239, 2323, 2885, 2893, 3067, 3498
Hsp92I	GRCGYC	2 cut(s)	168, 1623
HspAI	GCGC	1 cut(s)	538
Kpn2I	TCCGGA	1 cut(s)	203
LguI	GCTCTTC	1 cut(s)	1926
LmnI	GCTCC	7 cut(s)	665, 865, 961, 1604, 2310, 2914, 3232
MabI	ACCWGGT	1 cut(s)	1833
MaeI	CTAG	4 cut(s)	1964, 3005, 3236, 3284
MaeII	ACGT	9 cut(s)	163, 411, 2053, 2239, 2323, 2885, 2893, 3067, 3498
MaeIII	GTNAC	2 cut(s)	2131, 3026
MfeI	CAATTG	1 cut(s)	1536
MflI	RGATCY	7 cut(s)	69, 442, 2247, 2629, 2638, 3267, 3576
MlsI	TGGCCA	3 cut(s)	77, 1872, 2927
MluNI	TGGCCA	3 cut(s)	77, 1872, 2927
MlyI	GAGTC	4 cut(s)	553, 828, 1428, 1939
MmeI	TCCRAC	3 cut(s)	1110, 1435, 3586
Mox20I	TGGCCA	3 cut(s)	77, 1872, 2927
Mph1103I	ATGCAT	1 cut(s)	3619
MroI	TCCGGA	1 cut(s)	203
MroXI	GAANNNNTTC	1 cut(s)	1904
MscI	TGGCCA	3 cut(s)	77, 1872, 2927
MslI	CAYNNNNRTG	3 cut(s)	90, 930, 1706
Msp20I	TGGCCA	3 cut(s)	77, 1872, 2927
MspA1I	CMGCKG	6 cut(s)	90, 1253, 2291, 2333, 2780, 2821
MspCI	CTTAAG	2 cut(s)	2845, 3017
MspI	CCGG	2 cut(s)	204, 3218
MspR9I	CCNGG	5 cut(s)	1077, 1835, 1874, 2718, 3352
MunI	CAATTG	1 cut(s)	1536
Mva1269I	GAATGC	2 cut(s)	559, 2263
MvaI	CCWGG	5 cut(s)	1077, 1835, 1874, 2718, 3352
MwoI	GCNNNNNNNGC	7 cut(s)	1883, 2513, 2684, 2771, 3347, 3398, 3540
NcoI	CCATGG	2 cut(s)	65, 2299
NheI	GCTAGC	2 cut(s)	3235, 3283
NlaIV	GGNNCC	5 cut(s)	496, 1694, 2306, 2744, 2973
NmuCI	GTSAC	1 cut(s)	2131
NsiI	ATGCAT	1 cut(s)	3619
NspI	RCATGY	1 cut(s)	2774
NspV	TTCGAA	1 cut(s)	1069
OliI	CACNNNNGTG	1 cut(s)	90
PagI	TCATGA	3 cut(s)	307, 607, 2439
PceI	AGGCCT	1 cut(s)	1075
PciSI	GCTCTTC	1 cut(s)	1926
PctI	GAATGC	2 cut(s)	559, 2263
PdmI	GAANNNNTTC	1 cut(s)	1904
PfeI	GAWTC	8 cut(s)	883, 989, 1093, 1239, 1683, 2557, 2948, 3113
PflFI	GACNNNGTC	1 cut(s)	1418
Ple19I	CGATCG	1 cut(s)	676
PleI	GAGTC	4 cut(s)	552, 827, 1427, 1939
PpsI	GAGTC	4 cut(s)	552, 827, 1427, 1939
Ppu21I	YACGTR	5 cut(s)	164, 2886, 2894, 3068, 3499
PpuMI	RGGWCCY	2 cut(s)	1692, 2972
PshBI	ATTAAT	1 cut(s)	3146
Psp124BI	GAGCTC	1 cut(s)	1866
Psp1406I	AACGTT	1 cut(s)	2323
Psp5II	RGGWCCY	2 cut(s)	1692, 2972
Psp6I	CCWGG	5 cut(s)	1075, 1833, 1872, 2716, 3350
PspFI	CCCAGC	7 cut(s)	520, 920, 1249, 2780, 2821, 3374, 3447
PspGI	CCWGG	5 cut(s)	1075, 1833, 1872, 2716, 3350
PspN4I	GGNNCC	5 cut(s)	496, 1694, 2306, 2744, 2973
PspPI	GGNCC	7 cut(s)	155, 1247, 1692, 2047, 2424, 2742, 2972
PspPPI	RGGWCCY	2 cut(s)	1692, 2972
PsrI	GAACNNNNNNTAC	2 cut(s)	744, 776
PstI	CTGCAG	4 cut(s)	1406, 1440, 2779, 3545
PstNI	CAGNNNCTG	2 cut(s)	2516, 2912
PsuI	RGATCY	7 cut(s)	69, 442, 2247, 2629, 2638, 3267, 3576
PsyI	GACNNNGTC	1 cut(s)	1418
PvuI	CGATCG	1 cut(s)	676
PvuII	CAGCTG	5 cut(s)	1253, 2291, 2333, 2780, 2821
RsaI	GTAC	4 cut(s)	162, 998, 1712, 3539
RsaNI	GTAC	4 cut(s)	161, 997, 1711, 3538
RseI	CAYNNNNRTG	3 cut(s)	90, 930, 1706
SacI	GAGCTC	1 cut(s)	1866
SapI	GCTCTTC	1 cut(s)	1926
Sau96I	GGNCC	7 cut(s)	155, 1247, 1692, 2047, 2424, 2742, 2972
SchI	GAGTC	4 cut(s)	553, 828, 1428, 1939
ScrFI	CCNGG	5 cut(s)	1077, 1835, 1874, 2718, 3352
SexAI	ACCWGGT	1 cut(s)	1833
SfcI	CTRYAG	6 cut(s)	1402, 1436, 1892, 2508, 2775, 3541
SfuI	TTCGAA	1 cut(s)	1069
SinI	GGWCC	3 cut(s)	1692, 2742, 2972
SmiI	ATTTAAAT	1 cut(s)	2992
SmiMI	CAYNNNNRTG	3 cut(s)	90, 930, 1706
SmlI	CTYRAG	4 cut(s)	541, 2845, 3017, 3277
SmoI	CTYRAG	4 cut(s)	541, 2845, 3017, 3277
SnaBI	TACGTA	2 cut(s)	2886, 3068
SseBI	AGGCCT	1 cut(s)	1075
SsiI	CCGC	7 cut(s)	61, 90, 191, 694, 984, 1021, 2412
SspI	AATATT	2 cut(s)	1374, 2451
SspMI	CTAG	4 cut(s)	1964, 3005, 3236, 3284
SstI	GAGCTC	1 cut(s)	1866
StuI	AGGCCT	1 cut(s)	1075
StyD4I	CCNGG	5 cut(s)	1075, 1833, 1872, 2716, 3350
StyI	CCWWGG	6 cut(s)	65, 2001, 2299, 2364, 3043, 3076
SwaI	ATTTAAAT	1 cut(s)	2992
TaaI	ACNGT	8 cut(s)	137, 295, 475, 514, 1052, 1769, 2923, 3512
TaiI	ACGT	9 cut(s)	166, 414, 2056, 2242, 2326, 2888, 2896, 3070, 3501
TaqI	TCGA	9 cut(s)	15, 132, 676, 1069, 1753, 2032, 2790, 2862, 2903
TatI	WGTACW	2 cut(s)	996, 1710
TauI	GCSGC	2 cut(s)	64, 193
TfiI	GAWTC	8 cut(s)	883, 989, 1093, 1239, 1683, 2557, 2948, 3113
TscAI	CASTG	9 cut(s)	400, 1287, 1574, 1713, 2030, 2581, 2586, 3260, 3606
TseFI	GTSAC	1 cut(s)	2131
Tsp45I	GTSAC	1 cut(s)	2131
TspGWI	ACGGA	2 cut(s)	306, 1172
TspRI	CASTG	9 cut(s)	400, 1287, 1574, 1713, 2030, 2581, 2586, 3260, 3606
Tth111I	GACNNNGTC	1 cut(s)	1418
Vha464I	CTTAAG	2 cut(s)	2845, 3017
VneI	GTGCAC	1 cut(s)	1570
VpaK11BI	GGWCC	3 cut(s)	1692, 2742, 2972
VspI	ATTAAT	1 cut(s)	3146
XagI	CCTNNNNNAGG	2 cut(s)	2054, 2431
XapI	RAATTY	5 cut(s)	11, 2101, 2785, 2993, 3050
XceI	RCATGY	1 cut(s)	2774
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1744, 3358
XmnI	GAANNNNTTC	1 cut(s)	1904
XspI	CTAG	4 cut(s)	1964, 3005, 3236, 3284
Zsp2I	ATGCAT	1 cut(s)	3619

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.