Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000764

Physical Location & Seq

Genomic Coordinates Forward (+)

11364963 .. 11370881

Gene Structure 5919 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ29691

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3537 bp

ATGGGCACTCTCATCGACCGCTTTGACGAGCACGATGGCCCCGTCCGTGGCGTCCATTTCCACACCTCTCAGCCTCTCTTCGTCTCCGGAGGGGATGATTACAAGATCAAGGTGTGGAATTATAAGTTGCATAGGTGCCTGTTTACTCTTCTTGGACACCTTGATTACATCCGTACTGTTCAATTCCATCATGAGTACCCTTGGATTGTGAGTGCCAGTGATGACCAGACTATCAGAATATGGAATTGGCAGTCCCGGACTTGTATTTCTGTGCTCACTGGACACAACCATTATGTCATGTGTGCCTTGTTCCATCCCAAGGAGGATCTTGTTGTCTCGGCATCATTGGATCAAACTGTTCGTGTTTGGGATATTGGTGCACTCAGGAAGAAAACAGTTGCGCCTGCAGATGACATCCTACGACTGAGTCAAATGAATGCAGATTTCTTTGGTGGAGTTGATGCTGTGGTTAAGTATGTCTTGGAAGGCCATGATCGGGGTGTTAATTGGGCCTCCTTCCATCCTACACTCCCTCTGATTGTGTCTGGAGCTGATGATCGACAAGTGAAACTTTGGCGAATGAATGATACAAAGGCTTGGGAGGTGGACACTTTAAGGGGGCACATGAACAATGTATCATGTGTTTTATTCCATGCGAGGCAGGATATTATTGTTTCCAATTCAGAGGATAGGAGTATCCGAGTCTGGGATGCCACAAAAAGAACTGGTCTTCAGACATTTCGCAGAGAGCATGATAGGTTCTGGATTCTATCAGCACACCCTGAAATGAACCTTTTGGCTGCTGGTCATGATAGTGGCATGATTGTCTTTAAATTGGAGAGAGAACGCCCTGCCTTTTCTGTCAGTGCTGATTCTATGTACTATGTCAAAGATCGTTTTCTCCGCTTTTATGAGTTCTCAAACCAGAGAGACACTCAGGTTATCCCTATACGAAGGCCCGGTTCTTCCACCTTGAATCAAGGGGCTAAAACTCTATCTTATAGCCCTACAGAAAATGCTGTTTTGATCTGTTCAGATACGGAAGGCGGTTCCTATGAACTATACATAATTCCCAAAGAAAGCTATGGTCGGGGTGATACTGTGCAGGAGGCAAAAAGAGGAGCTGGAGGGCCAGCGGTTTTTGTGGCTCGAAATAGATTTGCTGTGCTTGAAAAAAGCACAAACCAAGTCTTAGTTAAAAATCTGAAAAATGAGATTATCAAGAAGAGCGCCCTTCCTTTTGTTGCTGATGCAATATACTATGCTGGAACTGGAAACTTGCTCTGCAGGGCAGAGGATAGAGTTGTCATTTTTGACCTGCAGCAAAGAATTATTCTTGGGGAGCTTCAAACTCCGTTTGTAAGGTACATAGTTTGGTCAAATGACATGGAGAACATTGCTTTACTCAGCAAACATTCCATTGTGATAGCCAATAAGAAGCTCGTTCATCAATGCACCCTACATGAGACCATTCGTGTAAAGAGTGGAGCTTGGGATGACCATGGTGTCTTCATATATACAACTCTGAACCATATCAAATACTGTCTTCCTAATGGGGACAGTGGAATCATTAGGACCCTTGATGTTCCCGTTTATATAACAAAGGTGTATGGAAGCACCATACATTGCTTGGATCGAGATGGGAAGAACTGTGCAATTGTTGTTGATGCAACAGAATATGTTTTTAAGTTGTCCTTACTGAAGAAGAGGTACGACCAGGTTATGAGCATGATTAGGAGCTCTGAGCTCTGTGGCCAGGCCATGATTGCATATCTACAGCAGAAAGGTTTCCCTGAGGTCGCCCTTCATTTTGTGAAGGATGAGAGGACTCGCTTCAACTTGGCACTAGGGAGTGGAAACATACAGATTGCTGTTGCTTCTGCTAAGGAAATTGATGAGAAAGATCACTGGTATCGGTTGGGAGTTGAGGCTCTTCGTCAAGGTAATTCAGGTATTGTAGAATATGCATATCAGAGGACAAAGAATTTTGAGAGGCTATCATTCCTATATCTTGTTACTGGAAACTTGGATAAGTTATCCAAAATGCTGAAAATCGCTGAAGTGAAGAATGATGTGATGGGCCAGTTCCACAATGCTCTATATTTGGGTGACATCGGGGAGCGTGTTAAGATATTGGAGAATGCTGGGCATTTACCCCTTGCATATGCTACAGCTAAAGCTCATGGCCTCCAAGATATTGCTGAACGGTTGGCAGGTGAGCTGGGAGATAATGTTCCTATTTTACCTGAAGGAAAGTCACCATCTCTTCTGGTACCTCCTAGCCCAATTATCTGTGGTGGAGATTGGCCCTTGTTGAGGGTCATGAGAGGAATATTTGAGGGTGGTCTTGATAATGTGGGCAGAAATGCTGAGGAAGAATATGAGGAGGCTGCAGATGCTGATTGGGGAGAGGATTTGGATATTGTTGATGTGGAAAACATTCAGAATGGAGACATTACTGCAGTGCTAGAAGATGAGGACACACATGAGGAAAATGAAGAGGGAGGGTGGGATCTTGAAGATCTTGAACTTCCACCGGAGATTGATACTCCCAAAACTGCCAACAATGCCCGTGCTTCCGTGTTTGTTGCCCCAACTCCAGGTATGCCTGTAAGTCAAATTTGGAGCCAAAAATCTTCTCTTGCTGCTGAACATGCAGCAGCTGGGAATTTTGATATTGCAATGCGATTATTGAGCCGGCAGCTGGGAATAAAGAACTTTGCACCTTTAAAACAATTGTTTCTTGACTTGCACACGGGGAGTCATTCATATTTACGCGCCTTATCGACAGCTCCTGTGATCTCCTTGGCTATTGAGAGGGGATGGAGTGAATCAGTAAGCCCTAATGTTAGGAGCCCTCCTGCTTTGGTTTTTAAATTCTCAGACCTGGAAGAGAAGCTTAAGGCGGGCTACAAGGCCACAACTACAGGGAAATTTACTGAGGCTTTACGACTTCTACTAGGTATTCTGCACACTATTCCTCTGGTTGTAGTTGATACAAGGAGAGAGGTTGATGAAGTAAAGGAATTAATTATCATTGTAAGGGAATATGTTCTGGGTTTGAAGATGGAGCTTAAGAGGAGGGAGGTTAAAGATAATCCAGTTCGTCAACAGGAGCTAGCAGCTTACTTCACCCACTGTAACCTCCAGATGCCTCACTTGAGACTAGCATTACTGAACGCCATGACTGTTTGCTACAAGGCTGGTAATCTCAACACAGCTGGTAACTTTGCCAGGCGACTATTGGAGACCAATCCCACAAATGAAAACCAAGCAAAGACTGCAAGGCAAGTCCTGCAGGCTGCTGAGAAGAACATGAATGATGCAACCCAGCTCAATTATGATTTCAGAAATCCATTTGTGGTTTGTGGGGCAACATATGTGCCTATTTACCGAGGACAGAAGGATGTGTCATGCCCGTACTGTAGTTCTCGGTTTGTTCCTGCTCAGGAAGGCCAGCTCTGTACTGTTTGCGATCTTGCAGTTGTTGGATCTGATGCATCTGGGTTACTCTGCTCTCCATCCCAGATAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COPI_C Coatomer_WDAD WD40

Protein Analysis

1178

Amino Acids

132.23

Weight (kDa)

6.23

Isoelectric Point (pI)

34.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40	PF00400	3 - 40	6.8e-06	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	6 - 123	1.3e-10	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	6 - 90	7.2e-12	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	7 - 125	1.4e-25	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	9 - 127	8.4e-31	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	9 - 128	4.3e-08	TEP-1 second beta-propeller
Beta-prop_EML	PF23409	10 - 124	8.9e-06	Echinoderm microtubule-associated protein first beta-propeller
WDR55	PF24796	10 - 127	1.9e-09	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	10 - 126	4.6e-16	WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	11 - 127	6.3e-08	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	11 - 124	2.5e-13	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	13 - 91	1.4e-09	WDHD1 first WD40 domain
WD40_Prp19	PF24814	17 - 127	3.2e-20	Prp19 WD40 domain
WD40	PF00400	45 - 82	2.9e-08	WD domain, G-beta repeat
Beta-prop_EIPR1	PF23609	52 - 123	1.9e-06	EIPR1 beta-propeller
WD40_WDHD1_1st	PF24817	57 - 127	3.9e-12	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	69 - 127	2.4e-12	THOC3 beta-propeller domain
WD40	PF00400	87 - 124	6.1e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	146 - 267	2.7e-06	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	156 - 275	1.9e-17	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	157 - 269	1.5e-20	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	157 - 278	3.9e-18	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	158 - 253	6.3e-07	TEP-1 second beta-propeller
WD40	PF00400	159 - 192	2.8e-06	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	163 - 275	6.2e-09	WDR3 second beta-propeller domain
WDR55	PF24796	168 - 276	9.4e-09	WDR55
Beta-prop_THOC3	PF25174	169 - 277	3.1e-14	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	172 - 248	6.7e-09	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	173 - 280	2.4e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	184 - 282	8.6e-07	CDC20/Fizzy WD40 domain
WD40	PF00400	202 - 237	1.6e-06	WD domain, G-beta repeat
B-prop_COPA_B_2nd	PF04053	301 - 545	2.3e-64	COPA/B second beta-propeller
TPR_COPA_B	PF23953	587 - 732	2.1e-18	COPA/B TPR domain
COPI_C	PF06957	776 - 1178	2.3e-172	Coatomer (COPI) alpha subunit C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62020 AT2G21390
fragaria_vesca	FvH4_3g10280 FvH4_5g12340 FvH4_5g12340 FvH4_5g12391 FvH4_5g12420
malus_domestica	MD05G1271000.v1.1 MD06G1214600.v1.1 MD10G1248600.v1.1 MD10G1248700.v1.1 MD10G1248800.v1.1 MD10G1249800.v1.1 MD10G1249900.v1.1 MD10G1250100.v1.1 MD10G1250200.v1.1 MD10G1250400.v1.1 MD10G1250600.v1.1 MD10G1250700.v1.1 MD10G1251000.v1.1 MD10G1251300.v1.1 MD10G1251600.v1.1 MD14G1225500.v1.1
prunus_persica	Prupe.4G091800_v2.0.a1 Prupe.4G091800_v2.0.a1 Prupe.5G220000_v2.0.a1 Prupe.5G220000_v2.0.a1
pyrus_communis	pycom05g25340 pycom06g19120 pycom10g20750 pycom14g18670
rosa_chinensis	RchiOBHm_Chr5g0016541 RchiOBHm_Chr7g0185121 RchiOBHm_Chr7g0185201
rosa_laevigata	RLG00000000634 RLG00000000671 RLG00000001958 RLG00000004933 RLG00000032256
rosa_multiflora	Rmu_co8505923.1_g000001 Rmu_co8507327.1_g000001 Rmu_sc0001235.1_g000018 Rmu_sc0004765.1_g000006 Rmu_sc0007192.1_g000011 Rmu_sc0019416.1_g000003 Rmu_ssc0000398.1_g000013
rosa_roxburghii	Rroxscaffold_1G00060340 Rroxscaffold_3G00269160 Rroxscaffold_3G00269190
rosa_rugosa	Rorug05G0032700 Rorug06G0466700 Rorug06G0466700 Rorug06G0466800 Rorug07G0188600
rosa_samantha	Rh4BG061900 Rh4CG068200 Rh4DG290700 Rh5AG127000 Rh5AG459900 Rh5BG125900 Rh5CG098400 Rh5CG138600 Rh5DG126100 Rh7AG076100 Rh7BG071200 Rh7CG072400 Rh7DG071500 Rh7DG071900
rosa_wichuraiana	Rw5G011000 Rw7G005980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	123
AarI	CACCTGC	1 cut(s)	2204
AasI	GACNNNNNNGTC	1 cut(s)	1505
Acc36I	ACCTGC	2 cut(s)	1326, 2204
Acc65I	GGTACC	1 cut(s)	2272
AccB1I	GGYRCC	2 cut(s)	135, 2272
AccII	CGCG	1 cut(s)	2778
AccIII	TCCGGA	1 cut(s)	86
AciI	CCGC	5 cut(s)	19, 904, 1047, 1136, 2906
AclWI	GGATC	5 cut(s)	333, 357, 1641, 2520, 3499
AcoI	YGGCCR	1 cut(s)	1753
AcsI	RAATTY	5 cut(s)	1984, 2619, 2668, 2876, 2933
AcuI	CTGAAG	4 cut(s)	716, 1721, 2079, 2268
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	8 cut(s)	175, 197, 881, 1367, 1712, 2274, 3422, 3466
AfiI	CCNNNNNNNGG	7 cut(s)	47, 319, 496, 706, 2316, 2600, 2704
AflII	CTTAAG	2 cut(s)	2900, 3074
AgsI	TTSAA	8 cut(s)	182, 976, 1172, 1349, 1837, 2519, 2528, 3064
AjnI	CCWGG	5 cut(s)	1716, 1755, 2599, 2886, 3233
AjuI	GAANNNNNNNTTGG	2 cut(s)	229, 261
AloI	GAACNNNNNNTCC	2 cut(s)	2217, 2249
Alw21I	GWGCWC	5 cut(s)	33, 276, 382, 1742, 1749
Alw26I	GTCTC	7 cut(s)	88, 340, 924, 1460, 2445, 3157, 3242
Alw44I	GTGCAC	1 cut(s)	378
AlwI	GGATC	5 cut(s)	333, 357, 1641, 2520, 3499
AlwNI	CAGNNNCTG	3 cut(s)	2399, 2663, 2795
Aor13HI	TCCGGA	1 cut(s)	86
ApaLI	GTGCAC	1 cut(s)	378
ApeKI	GCWGC	9 cut(s)	800, 1321, 2390, 2645, 2657, 2660, 2701, 3122, 3302
ApoI	RAATTY	5 cut(s)	1984, 2619, 2668, 2876, 2933
AseI	ATTAAT	1 cut(s)	3029
Asp700I	GAANNNNTTC	3 cut(s)	1787, 2439, 3051
Asp718I	GGTACC	1 cut(s)	2272
AspLEI	GCGC	3 cut(s)	403, 1232, 2780
AspS9I	GGNCC	7 cut(s)	38, 510, 957, 1130, 1575, 2080, 2307
AsuC2I	CCSGG	2 cut(s)	256, 960
AsuHPI	GGTGA	5 cut(s)	1106, 2120, 2228, 2250, 3124
AsuNHI	GCTAGC	1 cut(s)	3118
AvaII	GGWCC	1 cut(s)	1575
AxyI	CCTNAGG	1 cut(s)	1794
BaeGI	GKGCMC	3 cut(s)	8, 382, 624
BalI	TGGCCA	1 cut(s)	1755
BanI	GGYRCC	2 cut(s)	135, 2272
BanII	GRGCYC	3 cut(s)	1742, 1749, 2858
BarI	GAAGNNNNNNTAC	2 cut(s)	1694, 1726
BbsI	GAAGAC	3 cut(s)	722, 1501, 1538
Bbv12I	GWGCWC	5 cut(s)	33, 276, 382, 1742, 1749
BbvCI	CCTCAGC	1 cut(s)	2370
BbvI	GCAGC	9 cut(s)	787, 1333, 2377, 2632, 2669, 2672, 2713, 3134, 3289
BcgI	CGANNNNNNTGC	1 cut(s)	29
BciT130I	CCWGG	5 cut(s)	1718, 1757, 2601, 2888, 3235
BciVI	GTATCC	1 cut(s)	707
BcnI	CCSGG	2 cut(s)	256, 960
BcoDI	GTCTC	7 cut(s)	88, 340, 924, 1460, 2445, 3157, 3242
BfaI	CTAG	6 cut(s)	1847, 2280, 2468, 2960, 3119, 3167
BfoI	RGCGCY	1 cut(s)	1233
BfrI	CTTAAG	2 cut(s)	2900, 3074
BfuAI	ACCTGC	2 cut(s)	1326, 2204
BfuI	GTATCC	1 cut(s)	707
BglII	AGATCT	1 cut(s)	2521
BisI	GCNGC	9 cut(s)	801, 1322, 2391, 2646, 2658, 2661, 2702, 3123, 3303
BlsI	GCNGC	9 cut(s)	802, 1323, 2392, 2647, 2659, 2662, 2703, 3124, 3304
Bme1390I	CCNGG	7 cut(s)	256, 960, 1718, 1757, 2601, 2888, 3235
Bme18I	GGWCC	1 cut(s)	1575
BmgT120I	GGNCC	7 cut(s)	38, 510, 957, 1130, 1575, 2080, 2307
BmiI	GGNNCC	7 cut(s)	40, 137, 1051, 1577, 2274, 2627, 2855
BmrFI	CCNGG	7 cut(s)	256, 960, 1718, 1757, 2601, 2888, 3235
BmtI	GCTAGC	1 cut(s)	3122
BpiI	GAAGAC	3 cut(s)	722, 1501, 1538
BplI	GAGNNNNNCTC	2 cut(s)	919, 951
BpmI	CTGGAG	4 cut(s)	567, 1146, 2583, 3131
Bpu10I	CCTNAGC	3 cut(s)	1884, 2370, 3447
BpuEI	CTTGAG	1 cut(s)	3181
BpuMI	CCSGG	2 cut(s)	256, 960
BsaHI	GRCGYC	1 cut(s)	51
BsaI	GGTCTC	2 cut(s)	1460, 3242
BsaJI	CCNNGG	6 cut(s)	46, 200, 318, 1501, 2805, 3394
BsaWI	WCCGGW	2 cut(s)	86, 2536
BsaXI	ACNNNNNCTCC	2 cut(s)	2217, 2247
Bsc4I	CCNNNNNNNGG	7 cut(s)	47, 319, 496, 706, 2316, 2600, 2704
Bse118I	RCCGGY	1 cut(s)	2697
Bse1I	ACTGG	8 cut(s)	216, 283, 730, 1276, 1913, 2023, 2083, 3101
Bse21I	CCTNAGG	1 cut(s)	1794
Bse3DI	GCAATG	3 cut(s)	1395, 1624, 2688
BseAI	TCCGGA	1 cut(s)	86
BseBI	CCWGG	5 cut(s)	1718, 1757, 2601, 2888, 3235
BseDI	CCNNGG	6 cut(s)	46, 200, 318, 1501, 2805, 3394
BseLI	CCNNNNNNNGG	7 cut(s)	47, 319, 496, 706, 2316, 2600, 2704
BseMI	GCAATG	3 cut(s)	1395, 1624, 2688
BseNI	ACTGG	8 cut(s)	216, 283, 730, 1276, 1913, 2023, 2083, 3101
BseRI	GAGGAG	3 cut(s)	1134, 2399, 3094
BseSI	GKGCMC	3 cut(s)	8, 382, 624
BseXI	GCAGC	9 cut(s)	787, 1333, 2377, 2632, 2669, 2672, 2713, 3134, 3289
BseYI	CCCAGC	5 cut(s)	2144, 2221, 2663, 2704, 3330
BsgI	GTGCAG	2 cut(s)	1124, 2954
Bsh1236I	CGCG	1 cut(s)	2778
Bsh1285I	CGRYCG	1 cut(s)	19
BshNI	GGYRCC	2 cut(s)	135, 2272
BsiEI	CGRYCG	1 cut(s)	19
BsiHKAI	GWGCWC	5 cut(s)	33, 276, 382, 1742, 1749
BsiSI	CCGG	5 cut(s)	87, 256, 960, 2537, 2698
BslFI	GGGAC	2 cut(s)	238, 1571
BslI	CCNNNNNNNGG	7 cut(s)	47, 319, 496, 706, 2316, 2600, 2704
BsmAI	GTCTC	7 cut(s)	88, 340, 924, 1460, 2445, 3157, 3242
BsmBI	CGTCTC	1 cut(s)	88
BsmFI	GGGAC	2 cut(s)	238, 1571
BsmI	GAATGC	2 cut(s)	442, 2146
Bso31I	GGTCTC	2 cut(s)	1460, 3242
Bsp1286I	GDGCHC	8 cut(s)	8, 33, 276, 382, 624, 1742, 1749, 2858
Bsp13I	TCCGGA	1 cut(s)	86
Bsp19I	CCATGG	1 cut(s)	1501
BspACI	CCGC	5 cut(s)	19, 904, 1047, 1136, 2906
BspEI	TCCGGA	1 cut(s)	86
BspFNI	CGCG	1 cut(s)	2778
BspHI	TCATGA	3 cut(s)	190, 808, 2322
BspLI	GGNNCC	7 cut(s)	40, 137, 1051, 1577, 2274, 2627, 2855
BspMAI	CTGCAG	6 cut(s)	409, 1289, 1323, 2395, 2464, 3300
BspMI	ACCTGC	2 cut(s)	1326, 2204
BspOI	GCTAGC	1 cut(s)	3122
BspPI	GGATC	5 cut(s)	333, 357, 1641, 2520, 3499
BspQI	GCTCTTC	2 cut(s)	1220, 1938
BspT107I	GGYRCC	2 cut(s)	135, 2272
BspTI	CTTAAG	2 cut(s)	2900, 3074
BspTNI	GGTCTC	2 cut(s)	1460, 3242
BsrDI	GCAATG	3 cut(s)	1395, 1624, 2688
BsrFI	RCCGGY	1 cut(s)	2697
BsrI	ACTGG	8 cut(s)	216, 283, 730, 1276, 1913, 2023, 2083, 3101
BssAI	RCCGGY	1 cut(s)	2697
BssECI	CCNNGG	6 cut(s)	46, 200, 318, 1501, 2805, 3394
BssNI	GRCGYC	1 cut(s)	51
BssT1I	CCWWGG	4 cut(s)	200, 318, 1501, 2805
Bst2UI	CCWGG	5 cut(s)	1718, 1757, 2601, 2888, 3235
Bst6I	CTCTTC	8 cut(s)	83, 153, 1220, 1700, 1938, 2271, 2493, 2886
BstACI	GRCGYC	1 cut(s)	51
BstAFI	CTTAAG	2 cut(s)	2900, 3074
BstAPI	GCANNNNNTGC	2 cut(s)	3281, 3295
BstC8I	GCNNGC	7 cut(s)	405, 1134, 2699, 2908, 3120, 3300, 3458
BstDSI	CCRYGG	2 cut(s)	46, 1501
BstENI	CCTNNNNNAGG	1 cut(s)	2314
BstFNI	CGCG	1 cut(s)	2778
BstH2I	RGCGCY	1 cut(s)	1233
BstHHI	GCGC	3 cut(s)	403, 1232, 2780
BstMAI	GTCTC	7 cut(s)	88, 340, 924, 1460, 2445, 3157, 3242
BstMCI	CGRYCG	1 cut(s)	19
BstMWI	GCNNNNNNNGC	6 cut(s)	1766, 2396, 2567, 2654, 3281, 3295
BstNI	CCWGG	5 cut(s)	1718, 1757, 2601, 2888, 3235
BstNSI	RCATGY	1 cut(s)	2657
BstSCI	CCNGG	7 cut(s)	254, 958, 1716, 1755, 2599, 2886, 3233
BstSLI	GKGCMC	3 cut(s)	8, 382, 624
BstUI	CGCG	1 cut(s)	2778
BstV1I	GCAGC	9 cut(s)	787, 1333, 2377, 2632, 2669, 2672, 2713, 3134, 3289
BstV2I	GAAGAC	3 cut(s)	722, 1501, 1538
BstX2I	RGATCY	4 cut(s)	325, 2512, 2521, 3491
BstYI	RGATCY	4 cut(s)	325, 2512, 2521, 3491
Bsu36I	CCTNAGG	1 cut(s)	1794
BsuI	GTATCC	1 cut(s)	707
BtgI	CCRYGG	2 cut(s)	46, 1501
BtsI	GCAGTG	1 cut(s)	2469
BtsIMutI	CAGTG	7 cut(s)	223, 276, 871, 1567, 1906, 2469, 3136
BveI	ACCTGC	2 cut(s)	1326, 2204
Cac8I	GCNNGC	7 cut(s)	405, 1134, 2699, 2908, 3120, 3300, 3458
CaiI	CAGNNNCTG	3 cut(s)	2399, 2663, 2795
CciI	TCATGA	3 cut(s)	190, 808, 2322
CfoI	GCGC	3 cut(s)	403, 1232, 2780
Cfr10I	RCCGGY	1 cut(s)	2697
Cfr13I	GGNCC	7 cut(s)	38, 510, 957, 1130, 1575, 2080, 2307
CseI	GACGC	1 cut(s)	40
CsiI	ACCWGGT	1 cut(s)	1716
Csp6I	GTAC	8 cut(s)	174, 196, 880, 1366, 1711, 2273, 3421, 3465
CviQI	GTAC	8 cut(s)	174, 196, 880, 1366, 1711, 2273, 3421, 3465
DraI	TTTAAA	3 cut(s)	832, 2730, 2875
DrdI	GACNNNNNNGTC	1 cut(s)	1505
DseDI	GACNNNNNNGTC	1 cut(s)	1505
EaeI	YGGCCR	1 cut(s)	1753
Eam1104I	CTCTTC	8 cut(s)	83, 153, 1220, 1700, 1938, 2271, 2493, 2886
EarI	CTCTTC	8 cut(s)	83, 153, 1220, 1700, 1938, 2271, 2493, 2886
Ecl136II	GAGCTC	2 cut(s)	1740, 1747
Eco130I	CCWWGG	4 cut(s)	200, 318, 1501, 2805
Eco24I	GRGCYC	3 cut(s)	1742, 1749, 2858
Eco31I	GGTCTC	2 cut(s)	1460, 3242
Eco47I	GGWCC	1 cut(s)	1575
Eco53kI	GAGCTC	2 cut(s)	1740, 1747
Eco57I	CTGAAG	4 cut(s)	716, 1721, 2079, 2268
Eco81I	CCTNAGG	1 cut(s)	1794
EcoICRI	GAGCTC	2 cut(s)	1740, 1747
EcoNI	CCTNNNNNAGG	1 cut(s)	2314
EcoO109I	RGGNCCY	1 cut(s)	1575
EcoRII	CCWGG	5 cut(s)	1716, 1755, 2599, 2886, 3233
EcoT14I	CCWWGG	4 cut(s)	200, 318, 1501, 2805
EcoT22I	ATGCAT	2 cut(s)	1969, 3502
EcoT38I	GRGCYC	3 cut(s)	1742, 1749, 2858
ErhI	CCWWGG	4 cut(s)	200, 318, 1501, 2805
Esp3I	CGTCTC	1 cut(s)	88
FalI	AAGNNNNNCTT	8 cut(s)	464, 496, 555, 587, 1679, 1711, 2883, 2915
FaqI	GGGAC	2 cut(s)	238, 1571
FauI	CCCGC	1 cut(s)	2899
FauNDI	CATATG	2 cut(s)	2164, 3379
Fnu4HI	GCNGC	9 cut(s)	801, 1322, 2391, 2646, 2658, 2661, 2702, 3123, 3303
FriOI	GRGCYC	3 cut(s)	1742, 1749, 2858
Fsp4HI	GCNGC	9 cut(s)	801, 1322, 2391, 2646, 2658, 2661, 2702, 3123, 3303
FspBI	CTAG	6 cut(s)	1847, 2280, 2468, 2960, 3119, 3167
GlaI	GCGC	3 cut(s)	402, 1231, 2779
GluI	GCNGC	9 cut(s)	801, 1322, 2391, 2646, 2658, 2661, 2702, 3123, 3303
GsaI	CCCAGC	5 cut(s)	2148, 2225, 2667, 2708, 3334
GsuI	CTGGAG	4 cut(s)	567, 1146, 2583, 3131
HaeII	RGCGCY	1 cut(s)	1233
HapII	CCGG	5 cut(s)	87, 256, 960, 2537, 2698
HgaI	GACGC	1 cut(s)	40
HhaI	GCGC	3 cut(s)	403, 1232, 2780
Hin1I	GRCGYC	1 cut(s)	51
Hin6I	GCGC	3 cut(s)	401, 1230, 2778
HinP1I	GCGC	3 cut(s)	401, 1230, 2778
HincII	GTYRAC	1 cut(s)	3110
HindII	GTYRAC	1 cut(s)	3110
HindIII	AAGCTT	1 cut(s)	2897
HinfI	GANTC	9 cut(s)	427, 702, 766, 872, 976, 1566, 1828, 2761, 2831
HpaII	CCGG	5 cut(s)	87, 256, 960, 2537, 2698
HphI	GGTGA	5 cut(s)	1106, 2120, 2228, 2250, 3124
Hpy166II	GTNNAC	4 cut(s)	144, 380, 607, 3110
Hpy8I	GTNNAC	4 cut(s)	144, 380, 607, 3110
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1766, 2396, 2567, 2654, 3281, 3295
Hsp92I	GRCGYC	1 cut(s)	51
HspAI	GCGC	3 cut(s)	401, 1230, 2778
Kpn2I	TCCGGA	1 cut(s)	86
KpnI	GGTACC	1 cut(s)	2276
KroI	GCCGGC	1 cut(s)	2697
KroNI	GCCGGC	1 cut(s)	2699
LguI	GCTCTTC	2 cut(s)	1220, 1938
Lsp1109I	GCAGC	9 cut(s)	787, 1333, 2377, 2632, 2669, 2672, 2713, 3134, 3289
MabI	ACCWGGT	1 cut(s)	1716
MaeI	CTAG	6 cut(s)	1847, 2280, 2468, 2960, 3119, 3167
MaeIII	GTNAC	6 cut(s)	2014, 2108, 2256, 3140, 3224, 3507
MfeI	CAATTG	2 cut(s)	1656, 2735
MflI	RGATCY	4 cut(s)	325, 2512, 2521, 3491
MhlI	GDGCHC	8 cut(s)	8, 33, 276, 382, 624, 1742, 1749, 2858
MlsI	TGGCCA	1 cut(s)	1755
MluNI	TGGCCA	1 cut(s)	1755
MlyI	GAGTC	4 cut(s)	436, 711, 1822, 2770
MmeI	TCCRAC	1 cut(s)	3469
Mox20I	TGGCCA	1 cut(s)	1755
Mph1103I	ATGCAT	2 cut(s)	1969, 3502
MroI	TCCGGA	1 cut(s)	86
MroNI	GCCGGC	1 cut(s)	2697
MroXI	GAANNNNTTC	3 cut(s)	1787, 2439, 3051
MscI	TGGCCA	1 cut(s)	1755
MslI	CAYNNNNRTG	1 cut(s)	813
Msp20I	TGGCCA	1 cut(s)	1755
MspA1I	CMGCKG	4 cut(s)	1136, 2663, 2704, 3221
MspCI	CTTAAG	2 cut(s)	2900, 3074
MspI	CCGG	5 cut(s)	87, 256, 960, 2537, 2698
MspR9I	CCNGG	7 cut(s)	256, 960, 1718, 1757, 2601, 2888, 3235
MunI	CAATTG	2 cut(s)	1656, 2735
Mva1269I	GAATGC	2 cut(s)	442, 2146
MvaI	CCWGG	5 cut(s)	1718, 1757, 2601, 2888, 3235
MvnI	CGCG	1 cut(s)	2778
MwoI	GCNNNNNNNGC	6 cut(s)	1766, 2396, 2567, 2654, 3281, 3295
NaeI	GCCGGC	1 cut(s)	2699
NciI	CCSGG	2 cut(s)	256, 960
NcoI	CCATGG	1 cut(s)	1501
NdeI	CATATG	2 cut(s)	2164, 3379
NgoMIV	GCCGGC	1 cut(s)	2697
NheI	GCTAGC	1 cut(s)	3118
NlaIV	GGNNCC	7 cut(s)	40, 137, 1051, 1577, 2274, 2627, 2855
NmeAIII	GCCGAG	1 cut(s)	317
NmuCI	GTSAC	2 cut(s)	2108, 2256
NsiI	ATGCAT	2 cut(s)	1969, 3502
NspI	RCATGY	1 cut(s)	2657
PagI	TCATGA	3 cut(s)	190, 808, 2322
PaqCI	CACCTGC	1 cut(s)	2204
PciSI	GCTCTTC	2 cut(s)	1220, 1938
PcsI	WCGNNNNNNNCGW	1 cut(s)	39
PctI	GAATGC	2 cut(s)	442, 2146
PdiI	GCCGGC	1 cut(s)	2699
PdmI	GAANNNNTTC	3 cut(s)	1787, 2439, 3051
PfeI	GAWTC	5 cut(s)	766, 872, 976, 1566, 2831
PflFI	GACNNNGTC	1 cut(s)	426
PfoI	TCCNGGA	1 cut(s)	254
PkrI	GCNGC	9 cut(s)	802, 1323, 2392, 2647, 2659, 2662, 2703, 3124, 3304
PleI	GAGTC	4 cut(s)	435, 710, 1822, 2769
PpsI	GAGTC	4 cut(s)	435, 710, 1822, 2769
PpuMI	RGGWCCY	1 cut(s)	1575
PshBI	ATTAAT	1 cut(s)	3029
PsiI	TTATAA	1 cut(s)	123
Psp124BI	GAGCTC	2 cut(s)	1742, 1749
Psp5II	RGGWCCY	1 cut(s)	1575
Psp6I	CCWGG	5 cut(s)	1716, 1755, 2599, 2886, 3233
PspFI	CCCAGC	5 cut(s)	2144, 2221, 2663, 2704, 3330
PspGI	CCWGG	5 cut(s)	1716, 1755, 2599, 2886, 3233
PspN4I	GGNNCC	7 cut(s)	40, 137, 1051, 1577, 2274, 2627, 2855
PspPI	GGNCC	7 cut(s)	38, 510, 957, 1130, 1575, 2080, 2307
PspPPI	RGGWCCY	1 cut(s)	1575
PstI	CTGCAG	6 cut(s)	409, 1289, 1323, 2395, 2464, 3300
PstNI	CAGNNNCTG	3 cut(s)	2399, 2663, 2795
PsuI	RGATCY	4 cut(s)	325, 2512, 2521, 3491
PsyI	GACNNNGTC	1 cut(s)	426
PvuII	CAGCTG	3 cut(s)	2663, 2704, 3221
RsaI	GTAC	8 cut(s)	175, 197, 881, 1367, 1712, 2274, 3422, 3466
RsaNI	GTAC	8 cut(s)	174, 196, 880, 1366, 1711, 2273, 3421, 3465
RseI	CAYNNNNRTG	1 cut(s)	813
SacI	GAGCTC	2 cut(s)	1742, 1749
SapI	GCTCTTC	2 cut(s)	1220, 1938
SatI	GCNGC	9 cut(s)	801, 1322, 2391, 2646, 2658, 2661, 2702, 3123, 3303
Sau96I	GGNCC	7 cut(s)	38, 510, 957, 1130, 1575, 2080, 2307
SbfI	CCTGCAGG	1 cut(s)	3300
SchI	GAGTC	4 cut(s)	436, 711, 1822, 2770
ScrFI	CCNGG	7 cut(s)	256, 960, 1718, 1757, 2601, 2888, 3235
SdaI	CCTGCAGG	1 cut(s)	3300
SduI	GDGCHC	8 cut(s)	8, 33, 276, 382, 624, 1742, 1749, 2858
SexAI	ACCWGGT	1 cut(s)	1716
SinI	GGWCC	1 cut(s)	1575
SmiMI	CAYNNNNRTG	1 cut(s)	813
SmlI	CTYRAG	3 cut(s)	2900, 3074, 3160
SmoI	CTYRAG	3 cut(s)	2900, 3074, 3160
Sse8387I	CCTGCAGG	1 cut(s)	3300
SsiI	CCGC	5 cut(s)	19, 904, 1047, 1136, 2906
SspI	AATATT	1 cut(s)	2334
SspMI	CTAG	6 cut(s)	1847, 2280, 2468, 2960, 3119, 3167
SstI	GAGCTC	2 cut(s)	1742, 1749
StyD4I	CCNGG	7 cut(s)	254, 958, 1716, 1755, 2599, 2886, 3233
StyI	CCWWGG	4 cut(s)	200, 318, 1501, 2805
TaqI	TCGA	5 cut(s)	15, 559, 1150, 1636, 2786
TatI	WGTACW	2 cut(s)	879, 3464
TfiI	GAWTC	5 cut(s)	766, 872, 976, 1566, 2831
TscAI	CASTG	7 cut(s)	223, 283, 871, 1567, 1913, 2469, 3143
TseFI	GTSAC	2 cut(s)	2108, 2256
TseI	GCWGC	9 cut(s)	800, 1321, 2390, 2645, 2657, 2660, 2701, 3122, 3302
Tsp45I	GTSAC	2 cut(s)	2108, 2256
TspGWI	ACGGA	5 cut(s)	35, 161, 1055, 1344, 2569
TspRI	CASTG	7 cut(s)	223, 283, 871, 1567, 1913, 2469, 3143
Tth111I	GACNNNGTC	1 cut(s)	426
Vha464I	CTTAAG	2 cut(s)	2900, 3074
VneI	GTGCAC	1 cut(s)	378
VpaK11BI	GGWCC	1 cut(s)	1575
VspI	ATTAAT	1 cut(s)	3029
XagI	CCTNNNNNAGG	1 cut(s)	2314
XapI	RAATTY	5 cut(s)	1984, 2619, 2668, 2876, 2933
XceI	RCATGY	1 cut(s)	2657
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1627, 3241
XmnI	GAANNNNTTC	3 cut(s)	1787, 2439, 3051
XspI	CTAG	6 cut(s)	1847, 2280, 2468, 2960, 3119, 3167
Zsp2I	ATGCAT	2 cut(s)	1969, 3502

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.