Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000764

Physical Location & Seq

Genomic Coordinates Reverse (-)

34279938 .. 34281142

Gene Structure 1205 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1250100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1140 bp

ATGCCAATCATCTGCGGAGGAGATTGGCCCTTGTTGAGGGTCATGAGAGGAATATTTGAGGGTGGTCTTGATAATGTGGGCAAGAATATTGAGGAAGATTATGAAGAGGCTGCAGATGCTGATTGGGGAGAGGAGTTGGATCTTGTTGACATTGATACTCCAAAAACTGCCAGCAATGCCCGTTCTTCTGTGTTTGTTGCCCCAACTCCAGGTATGCCTGTAAGCCAAACCTGGTCCCAGAAATCATCTCTTGTTGCTGAACATGCTACTGCTGGGAATTTCGATATTGCAATGCGTTTGTTGAGCAGGGAGCTGGGTGTAAAGAACTTTGATCCCTTGAGACAATTATTTCTCGATTTACACATGGGCAGTCATATGTATCTACATGCCTTCTCGGCAGCTCATGTGCTCACCGTGGCCATTGAGAGGGGATGGAGTGAATCAGCTACACCTAAAGTTAGGGGTCCTCCTGCTCTTGTATTTAAATTCTCAGAGCTAGAAGAGAAGCTTAAGGCGGGTTACAAGGCCACAACGCAAGGGAAATTTGCTGATGCTTTACGTCTTCTCCTTGGTATTCTGTATACTATTCCTCTGATCGTAGTTGATTCAAAGAGAGAGGTTGATGAAGTAAAAGAATTAATTATCATTGTAAAGGAGTATGTTCTAGGTTTGAAGATGGAGATTAAGAGGAAGGAACTTAAAGGCGATCCGGTTCGCCAACAGGAGCTAGCTGCTTACTTCACCCACTGCAACCTTCAGATGCCTCACTTGAGACTTGCATTACTAAATGCCATGAGTGTTTGCTATAAGGCTGGTAATCTCAACACGGCAGCTAACTTTGCCAGGCGACTTTTGGAAACCAACCCCACCATTGAAAACCATGCAAAGACTGCAAGACAAATCCTGCAAGCTGCCGAAAAGAACATGACTGATGCAACCCAGCTAAACTATGATTTCAGAAACCCATTTGTCGTTTGTGGGGCAACATACTTGCCTATTTACCGTGGACAGAAGGATGTGTCATGCCCATACTGCAGTTCTCGGTTTGTTCCGGACCAGGAAGGGAAGCTCTGTTCTTTTGTGATCTTTCAGTGGTTGGATCAGATGCATCTGGTTTGCTCTGTTCTCCTACCCAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COPI_C Coatomer_WDAD WD40

Protein Analysis

380

Amino Acids

42.46

Weight (kDa)

6.33

Isoelectric Point (pI)

39.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
COPI_C	PF06957	33 - 359	1.6e-142	Coatomer (COPI) alpha subunit C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62020 AT2G21390
fragaria_vesca	FvH4_3g10280 FvH4_5g12340 FvH4_5g12340 FvH4_5g12391 FvH4_5g12420
malus_domestica	MD05G1271000.v1.1 MD06G1214600.v1.1 MD10G1248600.v1.1 MD10G1248700.v1.1 MD10G1248800.v1.1 MD10G1249800.v1.1 MD10G1249900.v1.1 MD10G1250100.v1.1 MD10G1250200.v1.1 MD10G1250400.v1.1 MD10G1250600.v1.1 MD10G1250700.v1.1 MD10G1251000.v1.1 MD10G1251300.v1.1 MD10G1251600.v1.1 MD14G1225500.v1.1
prunus_persica	Prupe.4G091800_v2.0.a1 Prupe.4G091800_v2.0.a1 Prupe.5G220000_v2.0.a1 Prupe.5G220000_v2.0.a1
pyrus_communis	pycom05g25340 pycom06g19120 pycom10g20750 pycom14g18670
rosa_chinensis	RchiOBHm_Chr5g0016541 RchiOBHm_Chr7g0185121 RchiOBHm_Chr7g0185201
rosa_laevigata	RLG00000000634 RLG00000000671 RLG00000001958 RLG00000004933 RLG00000032256
rosa_multiflora	Rmu_co8505923.1_g000001 Rmu_co8507327.1_g000001 Rmu_sc0001235.1_g000018 Rmu_sc0004765.1_g000006 Rmu_sc0007192.1_g000011 Rmu_sc0019416.1_g000003 Rmu_ssc0000398.1_g000013
rosa_roxburghii	Rroxscaffold_1G00060340 Rroxscaffold_3G00269160 Rroxscaffold_3G00269190
rosa_rugosa	Rorug05G0032700 Rorug06G0466700 Rorug06G0466700 Rorug06G0466800 Rorug07G0188600
rosa_samantha	Rh4BG061900 Rh4CG068200 Rh4DG290700 Rh5AG127000 Rh5AG459900 Rh5BG125900 Rh5CG098400 Rh5CG138600 Rh5DG126100 Rh7AG076100 Rh7BG071200 Rh7CG072400 Rh7DG071500 Rh7DG071900
rosa_wichuraiana	Rw5G011000 Rw7G005980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	581
AccIII	TCCGGA	1 cut(s)	1051
AciI	CCGC	2 cut(s)	15, 515
AclWI	GGATC	4 cut(s)	147, 326, 701, 1107
AcoI	YGGCCR	1 cut(s)	417
AcsI	RAATTY	3 cut(s)	277, 485, 542
AcuI	CTGAAG	1 cut(s)	740
AfiI	CCNNNNNNNGG	3 cut(s)	36, 209, 426
AflII	CTTAAG	1 cut(s)	509
AgsI	TTSAA	3 cut(s)	609, 673, 875
AjnI	CCWGG	4 cut(s)	208, 230, 842, 1056
AloI	GAACNNNNNNTCC	2 cut(s)	1057, 1089
Alw21I	GWGCWC	1 cut(s)	411
Alw26I	GTCTC	2 cut(s)	334, 766
AlwI	GGATC	4 cut(s)	147, 326, 701, 1107
AlwNI	CAGNNNCTG	1 cut(s)	119
Aor13HI	TCCGGA	1 cut(s)	1051
AoxI	GGCC	3 cut(s)	26, 417, 525
ApeKI	GCWGC	5 cut(s)	110, 398, 731, 830, 911
ApoI	RAATTY	3 cut(s)	277, 485, 542
AseI	ATTAAT	1 cut(s)	638
AspS9I	GGNCC	4 cut(s)	27, 234, 464, 1054
AsuHPI	GGTGA	2 cut(s)	403, 733
AsuNHI	GCTAGC	1 cut(s)	727
AvaII	GGWCC	3 cut(s)	234, 464, 1054
BalI	TGGCCA	1 cut(s)	419
BbsI	GAAGAC	1 cut(s)	554
Bbv12I	GWGCWC	1 cut(s)	411
BbvI	GCAGC	5 cut(s)	97, 410, 718, 842, 898
BccI	CCATC	2 cut(s)	426, 670
BceAI	ACGGC	1 cut(s)	843
BciT130I	CCWGG	4 cut(s)	210, 232, 844, 1058
BcoDI	GTCTC	2 cut(s)	334, 766
BfaI	CTAG	3 cut(s)	497, 665, 728
BfmI	CTRYAG	2 cut(s)	111, 1033
BfrI	CTTAAG	1 cut(s)	509
BglI	GCCNNNNNGGC	1 cut(s)	395
BisI	GCNGC	5 cut(s)	111, 399, 732, 831, 912
BlsI	GCNGC	5 cut(s)	112, 400, 733, 832, 913
Bme1390I	CCNGG	4 cut(s)	210, 232, 844, 1058
Bme18I	GGWCC	3 cut(s)	234, 464, 1054
BmgT120I	GGNCC	4 cut(s)	27, 234, 464, 1054
BmiI	GGNNCC	2 cut(s)	236, 465
BmrFI	CCNGG	4 cut(s)	210, 232, 844, 1058
BmsI	GCATC	6 cut(s)	106, 541, 750, 922, 1095, 1117
BmtI	GCTAGC	1 cut(s)	731
BpiI	GAAGAC	1 cut(s)	554
BpmI	CTGGAG	1 cut(s)	192
BpuEI	CTTGAG	2 cut(s)	358, 790
BsaJI	CCNNGG	3 cut(s)	414, 568, 1003
BsaWI	WCCGGW	2 cut(s)	709, 1051
BsaXI	ACNNNNNCTCC	2 cut(s)	549, 579
Bsc4I	CCNNNNNNNGG	3 cut(s)	36, 209, 426
Bse3DI	GCAATG	2 cut(s)	181, 297
BseAI	TCCGGA	1 cut(s)	1051
BseBI	CCWGG	4 cut(s)	210, 232, 844, 1058
BseDI	CCNNGG	3 cut(s)	414, 568, 1003
BseGI	GGATG	2 cut(s)	437, 1021
BseLI	CCNNNNNNNGG	3 cut(s)	36, 209, 426
BseMI	GCAATG	2 cut(s)	181, 297
BseMII	CTCAG	1 cut(s)	504
BseRI	GAGGAG	2 cut(s)	33, 146
BseXI	GCAGC	5 cut(s)	97, 410, 718, 842, 898
BseYI	CCCAGC	3 cut(s)	272, 313, 939
BshFI	GGCC	3 cut(s)	28, 419, 527
BsiHKAI	GWGCWC	1 cut(s)	411
BsiSI	CCGG	2 cut(s)	710, 1052
BslFI	GGGAC	1 cut(s)	220
BslI	CCNNNNNNNGG	3 cut(s)	36, 209, 426
BsmAI	GTCTC	2 cut(s)	334, 766
BsmFI	GGGAC	1 cut(s)	220
BsnI	GGCC	3 cut(s)	28, 419, 527
Bsp1286I	GDGCHC	1 cut(s)	411
Bsp13I	TCCGGA	1 cut(s)	1051
Bsp143I	GATC	6 cut(s)	139, 331, 594, 706, 1083, 1099
BspACI	CCGC	2 cut(s)	15, 515
BspANI	GGCC	3 cut(s)	28, 419, 527
BspCNI	CTCAG	1 cut(s)	503
BspEI	TCCGGA	1 cut(s)	1051
BspHI	TCATGA	1 cut(s)	42
BspLI	GGNNCC	2 cut(s)	236, 465
BspMAI	CTGCAG	2 cut(s)	115, 1037
BspOI	GCTAGC	1 cut(s)	731
BspPI	GGATC	4 cut(s)	147, 326, 701, 1107
BspTI	CTTAAG	1 cut(s)	509
BsrDI	GCAATG	2 cut(s)	181, 297
BssECI	CCNNGG	3 cut(s)	414, 568, 1003
BssMI	GATC	6 cut(s)	139, 331, 594, 706, 1083, 1099
BssNAI	GTATAC	1 cut(s)	582
BssT1I	CCWWGG	1 cut(s)	568
Bst1107I	GTATAC	1 cut(s)	582
Bst2UI	CCWGG	4 cut(s)	210, 232, 844, 1058
Bst4CI	ACNGT	2 cut(s)	415, 1004
Bst6I	CTCTTC	2 cut(s)	99, 495
BstAFI	CTTAAG	1 cut(s)	509
BstAPI	GCANNNNNTGC	1 cut(s)	890
BstC8I	GCNNGC	3 cut(s)	172, 729, 909
BstDEI	CTNAG	1 cut(s)	490
BstDSI	CCRYGG	2 cut(s)	414, 1003
BstENI	CCTNNNNNAGG	1 cut(s)	34
BstF5I	GGATG	2 cut(s)	437, 1021
BstKTI	GATC	6 cut(s)	142, 334, 597, 709, 1086, 1102
BstMAI	GTCTC	2 cut(s)	334, 766
BstMBI	GATC	6 cut(s)	139, 331, 594, 706, 1083, 1099
BstMWI	GCNNNNNNNGC	7 cut(s)	116, 176, 263, 395, 839, 890, 1032
BstNI	CCWGG	4 cut(s)	210, 232, 844, 1058
BstNSI	RCATGY	2 cut(s)	266, 389
BstSCI	CCNGG	4 cut(s)	208, 230, 842, 1056
BstSFI	CTRYAG	2 cut(s)	111, 1033
BstV1I	GCAGC	5 cut(s)	97, 410, 718, 842, 898
BstV2I	GAAGAC	1 cut(s)	554
BstX2I	RGATCY	1 cut(s)	139
BstYI	RGATCY	1 cut(s)	139
BstZ17I	GTATAC	1 cut(s)	582
BsuRI	GGCC	3 cut(s)	28, 419, 527
BtgI	CCRYGG	2 cut(s)	414, 1003
BtsCI	GGATG	2 cut(s)	437, 1021
BtsI	GCAGTG	1 cut(s)	745
BtsIMutI	CAGTG	2 cut(s)	745, 1097
Cac8I	GCNNGC	3 cut(s)	172, 729, 909
CaiI	CAGNNNCTG	1 cut(s)	119
CciI	TCATGA	1 cut(s)	42
Cfr13I	GGNCC	4 cut(s)	27, 234, 464, 1054
CsiI	ACCWGGT	1 cut(s)	230
CviAII	CATG	9 cut(s)	43, 263, 364, 386, 404, 793, 881, 925, 1023
DdeI	CTNAG	1 cut(s)	490
DpnI	GATC	6 cut(s)	141, 333, 596, 708, 1085, 1101
DpnII	GATC	6 cut(s)	139, 331, 594, 706, 1083, 1099
DraI	TTTAAA	1 cut(s)	484
EaeI	YGGCCR	1 cut(s)	417
Eam1104I	CTCTTC	2 cut(s)	99, 495
EarI	CTCTTC	2 cut(s)	99, 495
Eco130I	CCWWGG	1 cut(s)	568
Eco47I	GGWCC	3 cut(s)	234, 464, 1054
Eco57I	CTGAAG	1 cut(s)	740
EcoNI	CCTNNNNNAGG	1 cut(s)	34
EcoO109I	RGGNCCY	1 cut(s)	464
EcoRII	CCWGG	4 cut(s)	208, 230, 842, 1056
EcoT14I	CCWWGG	1 cut(s)	568
EcoT22I	ATGCAT	1 cut(s)	1110
ErhI	CCWWGG	1 cut(s)	568
FaeI	CATG	9 cut(s)	46, 266, 367, 389, 407, 796, 884, 928, 1026
FalI	AAGNNNNNCTT	2 cut(s)	492, 524
FaqI	GGGAC	1 cut(s)	220
FatI	CATG	9 cut(s)	42, 262, 363, 385, 403, 792, 880, 924, 1022
FauI	CCCGC	1 cut(s)	508
FauNDI	CATATG	1 cut(s)	375
FblI	GTMKAC	1 cut(s)	581
Fnu4HI	GCNGC	5 cut(s)	111, 399, 732, 831, 912
FokI	GGATG	2 cut(s)	444, 1028
Fsp4HI	GCNGC	5 cut(s)	111, 399, 732, 831, 912
FspBI	CTAG	3 cut(s)	497, 665, 728
GluI	GCNGC	5 cut(s)	111, 399, 732, 831, 912
GsaI	CCCAGC	3 cut(s)	276, 317, 943
GsuI	CTGGAG	1 cut(s)	192
HaeIII	GGCC	3 cut(s)	28, 419, 527
HapII	CCGG	2 cut(s)	710, 1052
Hin1II	CATG	9 cut(s)	46, 266, 367, 389, 407, 796, 884, 928, 1026
HincII	GTYRAC	1 cut(s)	148
HindII	GTYRAC	1 cut(s)	148
HindIII	AAGCTT	1 cut(s)	506
HinfI	GANTC	2 cut(s)	440, 605
HpaII	CCGG	2 cut(s)	710, 1052
HphI	GGTGA	2 cut(s)	403, 733
Hpy166II	GTNNAC	3 cut(s)	148, 582, 1007
Hpy188I	TCNGA	5 cut(s)	493, 594, 759, 959, 1104
Hpy188III	TCNNGA	4 cut(s)	43, 68, 353, 1052
Hpy8I	GTNNAC	3 cut(s)	148, 582, 1007
HpyAV	CCTTC	5 cut(s)	400, 685, 764, 1006, 1055
HpyCH4III	ACNGT	2 cut(s)	415, 1004
HpyCH4IV	ACGT	1 cut(s)	559
HpyF10VI	GCNNNNNNNGC	7 cut(s)	116, 176, 263, 395, 839, 890, 1032
HpyF3I	CTNAG	1 cut(s)	490
HpySE526I	ACGT	1 cut(s)	559
Hsp92II	CATG	9 cut(s)	46, 266, 367, 389, 407, 796, 884, 928, 1026
Kpn2I	TCCGGA	1 cut(s)	1051
Kzo9I	GATC	6 cut(s)	139, 331, 594, 706, 1083, 1099
LmnI	GCTCC	2 cut(s)	310, 724
Lsp1109I	GCAGC	5 cut(s)	97, 410, 718, 842, 898
LweI	GCATC	6 cut(s)	106, 541, 750, 922, 1095, 1117
MabI	ACCWGGT	1 cut(s)	230
MaeI	CTAG	3 cut(s)	497, 665, 728
MaeII	ACGT	1 cut(s)	559
MaeIII	GTNAC	1 cut(s)	518
MalI	GATC	6 cut(s)	141, 333, 596, 708, 1085, 1101
MboI	GATC	6 cut(s)	139, 331, 594, 706, 1083, 1099
MboII	GAAGA	6 cut(s)	107, 116, 177, 512, 554, 685
MflI	RGATCY	1 cut(s)	139
MhlI	GDGCHC	1 cut(s)	411
MlsI	TGGCCA	1 cut(s)	419
MluCI	AATT	6 cut(s)	277, 344, 485, 542, 635, 639
MluNI	TGGCCA	1 cut(s)	419
MmeI	TCCRAC	2 cut(s)	117, 1077
Mox20I	TGGCCA	1 cut(s)	419
Mph1103I	ATGCAT	1 cut(s)	1110
MroI	TCCGGA	1 cut(s)	1051
MscI	TGGCCA	1 cut(s)	419
MseI	TTAA	5 cut(s)	483, 510, 638, 684, 699
Msp20I	TGGCCA	1 cut(s)	419
MspCI	CTTAAG	1 cut(s)	509
MspI	CCGG	2 cut(s)	710, 1052
MspR9I	CCNGG	4 cut(s)	210, 232, 844, 1058
MvaI	CCWGG	4 cut(s)	210, 232, 844, 1058
MwoI	GCNNNNNNNGC	7 cut(s)	116, 176, 263, 395, 839, 890, 1032
NdeI	CATATG	1 cut(s)	375
NdeII	GATC	6 cut(s)	139, 331, 594, 706, 1083, 1099
NheI	GCTAGC	1 cut(s)	727
NlaIII	CATG	9 cut(s)	46, 266, 367, 389, 407, 796, 884, 928, 1026
NlaIV	GGNNCC	2 cut(s)	236, 465
NmeAIII	GCCGAG	1 cut(s)	374
NsiI	ATGCAT	1 cut(s)	1110
NspI	RCATGY	2 cut(s)	266, 389
PagI	TCATGA	1 cut(s)	42
PfeI	GAWTC	2 cut(s)	440, 605
PkrI	GCNGC	5 cut(s)	112, 400, 733, 832, 913
PpuMI	RGGWCCY	1 cut(s)	464
PshBI	ATTAAT	1 cut(s)	638
Psp5II	RGGWCCY	1 cut(s)	464
Psp6I	CCWGG	4 cut(s)	208, 230, 842, 1056
PspFI	CCCAGC	3 cut(s)	272, 313, 939
PspGI	CCWGG	4 cut(s)	208, 230, 842, 1056
PspN4I	GGNNCC	2 cut(s)	236, 465
PspPI	GGNCC	4 cut(s)	27, 234, 464, 1054
PspPPI	RGGWCCY	1 cut(s)	464
PstI	CTGCAG	2 cut(s)	115, 1037
PstNI	CAGNNNCTG	1 cut(s)	119
PsuI	RGATCY	1 cut(s)	139
SaqAI	TTAA	5 cut(s)	483, 510, 638, 684, 699
SatI	GCNGC	5 cut(s)	111, 399, 732, 831, 912
Sau3AI	GATC	6 cut(s)	139, 331, 594, 706, 1083, 1099
Sau96I	GGNCC	4 cut(s)	27, 234, 464, 1054
ScrFI	CCNGG	4 cut(s)	210, 232, 844, 1058
SduI	GDGCHC	1 cut(s)	411
SexAI	ACCWGGT	1 cut(s)	230
SfaNI	GCATC	6 cut(s)	106, 541, 750, 922, 1095, 1117
SfcI	CTRYAG	2 cut(s)	111, 1033
SinI	GGWCC	3 cut(s)	234, 464, 1054
SmiI	ATTTAAAT	1 cut(s)	484
SmlI	CTYRAG	3 cut(s)	337, 509, 769
SmoI	CTYRAG	3 cut(s)	337, 509, 769
Sse9I	AATT	6 cut(s)	277, 344, 485, 542, 635, 639
SsiI	CCGC	2 cut(s)	15, 515
SspI	AATATT	2 cut(s)	54, 88
SspMI	CTAG	3 cut(s)	497, 665, 728
StyD4I	CCNGG	4 cut(s)	208, 230, 842, 1056
StyI	CCWWGG	1 cut(s)	568
SwaI	ATTTAAAT	1 cut(s)	484
TaaI	ACNGT	2 cut(s)	415, 1004
TaiI	ACGT	1 cut(s)	562
TaqI	TCGA	2 cut(s)	282, 354
TasI	AATT	6 cut(s)	277, 344, 485, 542, 635, 639
TfiI	GAWTC	2 cut(s)	440, 605
Tru1I	TTAA	5 cut(s)	483, 510, 638, 684, 699
Tru9I	TTAA	5 cut(s)	483, 510, 638, 684, 699
TscAI	CASTG	2 cut(s)	752, 1097
TseI	GCWGC	5 cut(s)	110, 398, 731, 830, 911
TspDTI	ATGAA	2 cut(s)	117, 639
TspRI	CASTG	2 cut(s)	752, 1097
Vha464I	CTTAAG	1 cut(s)	509
VpaK11BI	GGWCC	3 cut(s)	234, 464, 1054
VspI	ATTAAT	1 cut(s)	638
XagI	CCTNNNNNAGG	1 cut(s)	34
XapI	RAATTY	3 cut(s)	277, 485, 542
XceI	RCATGY	2 cut(s)	266, 389
XcmI	CCANNNNNNNNNTGG	1 cut(s)	850
XmiI	GTMKAC	1 cut(s)	581
XspI	CTAG	3 cut(s)	497, 665, 728
Zsp2I	ATGCAT	1 cut(s)	1110

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.