Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0000764

Physical Location & Seq

Genomic Coordinates Reverse (-)

26726570 .. 26731419

Gene Structure 4850 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g25340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3582 bp

ATGGCCAGTCTCCACAGCGGTGCGATCCAGCTCTGGGATTACCGAATGGGCACTCTCATCTACCGTTTTGACGAGCACGATGGCCCTGTACGTGGCGTCCATTTCCACTCCTCTCAGCCGCTTTTCGTCTCCGGAGGGGATGATTACAAGATCAAGGTATGGAATTACAAGTTGCATAGGTGTTTGTTTACACTTCTTGGACACCTTGATTACATCCGAACCGTTCAATTCCATCATGAGTACCCGTGGATTGTTAGTGCGAGTGATGACCAGACAATTAGAATATGGAATTGGCAGTCCCGAACTTGTATTTCTGTGCTCACTGGACACAACCATTACGTCATGTGTGCCTTATTCCATCCAAAGGAGGATCTTGTTGTCTCAGCATCATTGGATCAAACTGTTCGTGTTTGGGATATTGGTGCCCTAAGGAAAAAGACGGTTGCCCCAGCAGATGATATCATGCGCTTGAGTCAGATGAATGCAGATTTCTTTGGTGGAGTTGATGCTGTTGTGAAGTATGTTTTGGAAGGTCATGATCGGGGTGTGAATTGGGCATCATTTCATCCTACACTCCCTCTGATCGTCTCTGGAGCAGACGATCGACAATTGAAACTTTGGCGGATGAATGATACAAAGGCTTGGGAAGTGGACACTCTAAGGGGGCACATGAACAATGTATCCTGTGTTCTATTCCATTCAAGGCAGGATATTATTGTGTCCAATTCCGAGGATAGGAGTATCCGAGTCTGGGATGCAACAAAACGGACTGGTCTTCAGACATTTCGTAGGGAGCATGATAGGTTCTGGATTCTTTCAGCACATCCTGAAATGAACCTCTTGGCTGCTGGGCATGATAGTGGCATGATCGTCTTTAAATTGGAGAGGGAGCGTCCTGCTTTTTCTGTTAGCGGTGATTCCATGTACTATGTTAAAGATCGTTTTCTCCGCTTCTATGAGTTCTCCACCCAGAGAGACAGTCAAGTTATCCCAATTCGAAGGCCTGGTTCTTCCAACTTGAATCAAGGGGCAAAAACACTATCTTACAGCCCTACTGAAAATGCTGTTTTGATAGGTTCAGATACGGAAGGGGGTTCTTATGAACTATACATTATACCCAAAGATAGCTTTGGTCGGGGTGATACTGTGCAGGAGGCAAAAAGAGGAATCGGAGGCCCAGCTGTGTTCGTGGCTCGCAATAGGTTTGCAGTGCTTGAAAAGAGCACCAACCAAGTTATAGTTAAAAATCTGAAAAATGAGATTGTCAAGAAGAGTGCCCTACCTATTGTTGCTGATGCAATATTTTATGCTGGAACTGGAAACTTGCTCTGCAGGGCAGAGGACAGAGTCATTATTTTTGACCTGCAGCAGAGAATTATTGTTGGAGAGCTTCAAACTCCTTTTGTAAGGTATATTGTTTGGTCAAATGACATGGAGAGCATTGCTTTACTTAGCAAACATTCAATTGTGATAGCCAATAAGAAGCTTGTGCATCAGTGCACTCTCCATGAGACAATTCGTGTAAAGAGTGGAGCTTGGGATGACAATGGTGTCTTCATATATACAACCTTGAATCACATCAAATACTGCCTTCCTAATGGGGATAGTGGAATCATCAGGACCCTTGACATTCCCGTGTATATAACAAAAGTGTATGGAAACACCATACATTGCTTGGATCGAGATGGGAAGAACCGTGCCATTGTTGTTGATGCAACAGAATATGTTTTCAAGTTATCCTTACTGAAGAAAAGGTATGACCAGGTTATGAGCATGATTAGGAGTTCTGAGCTCTGTGGCCAGGCCATGATTGCATATCTACAGCAGAAAGGTTTCCCTGAAGTTGCTCTTCATTTTGTGAAGGATGAGAGGACTCGCTTCAACTTGGCACTAGGGAGTGGGAACATCCAGATTGCTGTTGCTTCTGCCAAGGAAATTGATGAGAAAGATCACTGGTATCGATTGGGAGTGGAGGCCCTACGTCAGGGTAATGCAGGCATTGTAGAATATGCGTATCAGAGGACAAAGAATTTTGAGAGGCTATCATTCCTATATCTTGTGACTGGAAACTTGGATAAATTGTCCAAAATGCTGAAAATTGCTGAAGTGAAGAATGATGTGATGGGTCAGTTCCACAATGCTTTGTATTTGGGTGATATCAGAGAACGTGTTAAGATCTTGGAGAATGCGGGTCATTTAGCTCTTGCTTATAGCACAGCTGTAATCCATGGGCTCCATGATATTGCTGAACGTTTGGCAGCTGAACTGGGAGATAATGTTCCTGTTTTACCCAAGGGGAAATCTCCCTCTCTTTTGATACCTCCAACGCCAATCATCTGCGGTGGAGATTGGCCCTTGTTGAGGGTCATGAGAGGAATATTTGAGGGTGGTCTTGATAATGTGGGGAAGAATGTTGAGGAAGAGTATGAAGAGGCTACAGATGCTGATTGGGGAGAGGATTTGGATATTGTTGACGTGGACAAGATTCCAAATGGAGACATCAGTGCAGTGCTGGATGATGAGGAAGCACACGAGGAAAATGAGGAGGAAGGATGGGATCTTGAAGATCTTGAACTTCCGCCTGAGATTGATACTCCAAAAACTGCCAGCAACGCCCGTTCTTCTGTGTTTGTTGCCCCAACTCCTGGTATGCCTGTAAGTCAAATCTGGTCCCAGAAATCATCTCTGGCTGCTGAACATGCTGCAGCCGGGAATTTTGATATTGCAATGCGTTTGTTGAGCAGGCAGCTGGGTATAAAAAACTTTGATCCCTTAAGACAATTATTTCTCGATTTACACATGGGCAGTCATACGTATCTACGTGCCTTCTCGACAGCTCCTGTGCTTACCGTGGCCATTGAGAGGGGATGGAGTGAATCAGCTACTCCTAATGTTAGGGGTCCTCCTGCTCTTGTATTTAAATTCTCTGAGCTAGAAGAGAAGCTTAAGGTGGGTTACAAGGCCACAACCCAAGGGAAATTTGCTGATGCTTTACGTCTTTTCCTTGGTATTCTGCATACTATTCCTCTGATTGTAGTTGATTCAAGGAGAGAGGTTGATGAAGTAAAAGAATTGATAATCATTGTAAAGGAGTATGTTCTGGGTTTGAAGATGGAGATTAAGAGGAAGGAACTTAAAGGCGATCCGGTTCGCCAACAGGAGCTGGCAGCTTACTTCACCCACTGCAACCTTCAGATCCCTCACTTGAGGCTAGCATTACTAAATGCCATGAGTGTTTGCTATAAGGCTGGTAATCTCAACACGGCAGCTAACTTTGCCAGGCGACTTTTGGAGACCAACCCCAGCGTTGAAAACCATGCAAAGACTGCAAGACAAATCCTGCAAGCTGCCGAAAAGAACATGACTGATGCAACCCAGCTAAACTATGACTTCAGAAACCCATTTGTGGTTTGTGGGGGAACATACGTGCCTATTTACCGTGGACAGAAGGATGTGTCATGCCCGTACTGCAGCTCTCGGTTTGTTCCTGAGCATGAAGGAAAGATCTGTACTGTTTGTGATCTTGCAGTGGTTGGATCAGATGCATCTGGTTTGCTCTGTTCTCCTACCCAGATAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COPI_C Coatomer_WDAD WD40

Protein Analysis

1194

Amino Acids

134.2

Weight (kDa)

6.42

Isoelectric Point (pI)

31.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	5 - 103	1.1e-14	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	5 - 140	5.2e-30	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	6 - 141	1.3e-16	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	6 - 138	3.3e-09	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	7 - 138	2.6e-13	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	8 - 86	1.7e-11	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	8 - 98	3.5e-07	G protein beta WD-40 repeat protein
WDR55	PF24796	8 - 142	1.2e-10	WDR55
WD40	PF00400	17 - 55	9.7e-07	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	26 - 142	6.9e-08	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	36 - 143	5.9e-20	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	39 - 141	3.1e-07	TEP-1 second beta-propeller
WD40	PF00400	60 - 97	2.9e-08	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	64 - 139	1.6e-08	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	64 - 142	7.7e-23	WDR5 beta-propeller domain
Beta-prop_EIPR1	PF23609	67 - 138	1.9e-06	EIPR1 beta-propeller
WD40_WDHD1_1st	PF24817	72 - 142	3.4e-12	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	84 - 143	2.4e-12	THOC3 beta-propeller domain
WD40	PF00400	102 - 139	6.2e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	166 - 277	9.4e-06	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	171 - 290	2.5e-16	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	172 - 284	5.2e-20	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	172 - 293	9.3e-18	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	173 - 268	3.4e-07	TEP-1 second beta-propeller
WD40	PF00400	174 - 207	7.9e-06	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	178 - 287	1.1e-08	WDR3 second beta-propeller domain
WDR55	PF24796	183 - 291	5.9e-08	WDR55
Beta-prop_THOC3	PF25174	184 - 291	1.2e-13	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	187 - 262	1.7e-08	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	188 - 295	3.3e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	200 - 296	2.2e-06	CDC20/Fizzy WD40 domain
WD40	PF00400	217 - 252	8.2e-07	WD domain, G-beta repeat
B-prop_COPA_B_2nd	PF04053	316 - 560	4.5e-66	COPA/B second beta-propeller
TPR_COPA_B	PF23953	602 - 736	1.5e-17	COPA/B TPR domain
COPI_C	PF06957	791 - 1193	2.3e-173	Coatomer (COPI) alpha subunit C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62020 AT2G21390
fragaria_vesca	FvH4_3g10280 FvH4_5g12340 FvH4_5g12340 FvH4_5g12391 FvH4_5g12420
malus_domestica	MD05G1271000.v1.1 MD06G1214600.v1.1 MD10G1248600.v1.1 MD10G1248700.v1.1 MD10G1248800.v1.1 MD10G1249800.v1.1 MD10G1249900.v1.1 MD10G1250100.v1.1 MD10G1250200.v1.1 MD10G1250400.v1.1 MD10G1250600.v1.1 MD10G1250700.v1.1 MD10G1251000.v1.1 MD10G1251300.v1.1 MD10G1251600.v1.1 MD14G1225500.v1.1
prunus_persica	Prupe.4G091800_v2.0.a1 Prupe.4G091800_v2.0.a1 Prupe.5G220000_v2.0.a1 Prupe.5G220000_v2.0.a1
pyrus_communis	pycom05g25340 pycom06g19120 pycom10g20750 pycom14g18670
rosa_chinensis	RchiOBHm_Chr5g0016541 RchiOBHm_Chr7g0185121 RchiOBHm_Chr7g0185201
rosa_laevigata	RLG00000000634 RLG00000000671 RLG00000001958 RLG00000004933 RLG00000032256
rosa_multiflora	Rmu_co8505923.1_g000001 Rmu_co8507327.1_g000001 Rmu_sc0001235.1_g000018 Rmu_sc0004765.1_g000006 Rmu_sc0007192.1_g000011 Rmu_sc0019416.1_g000003 Rmu_ssc0000398.1_g000013
rosa_roxburghii	Rroxscaffold_1G00060340 Rroxscaffold_3G00269160 Rroxscaffold_3G00269190
rosa_rugosa	Rorug05G0032700 Rorug06G0466700 Rorug06G0466700 Rorug06G0466800 Rorug07G0188600
rosa_samantha	Rh4BG061900 Rh4CG068200 Rh4DG290700 Rh5AG127000 Rh5AG459900 Rh5BG125900 Rh5CG098400 Rh5CG138600 Rh5DG126100 Rh7AG076100 Rh7BG071200 Rh7CG072400 Rh7DG071500 Rh7DG071900
rosa_wichuraiana	Rw5G011000 Rw7G005980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1550
Acc36I	ACCTGC	1 cut(s)	1371
AccB1I	GGYRCC	1 cut(s)	422
AccB7I	CCANNNNNTGG	1 cut(s)	2645
AccIII	TCCGGA	1 cut(s)	131
AciI	CCGC	8 cut(s)	18, 119, 622, 912, 949, 2189, 2340, 2579
AclI	AACGTT	1 cut(s)	2251
AclWI	GGATC	9 cut(s)	19, 378, 402, 1686, 2565, 2762, 3137, 3190, 3544
AcoI	YGGCCR	3 cut(s)	3, 1798, 2853
AcsI	RAATTY	4 cut(s)	2029, 2713, 2921, 2978
AcuI	CTGAAG	6 cut(s)	761, 1766, 1860, 2124, 3176, 3376
AcyI	GRCGYC	1 cut(s)	96
AfaI	GTAC	5 cut(s)	90, 242, 926, 3467, 3511
AflII	CTTAAG	2 cut(s)	2773, 2945
AflIII	ACRYGT	1 cut(s)	2167
AjiI	CACGTC	1 cut(s)	2476
AjnI	CCWGG	5 cut(s)	1003, 1761, 1800, 2644, 3278
AjuI	GAANNNNNNNTTGG	4 cut(s)	274, 306, 509, 541
AleI	CACNNNNGTG	1 cut(s)	18
AloI	GAACNNNNNNTCC	4 cut(s)	665, 697, 2262, 2294
Alw21I	GWGCWC	5 cut(s)	78, 321, 1226, 1502, 1794
Alw26I	GTCTC	8 cut(s)	14, 133, 385, 592, 969, 1505, 2490, 3287
Alw44I	GTGCAC	1 cut(s)	1498
AlwI	GGATC	9 cut(s)	19, 378, 402, 1686, 2565, 2762, 3137, 3190, 3544
AlwNI	CAGNNNCTG	3 cut(s)	2444, 2840, 3163
Aor13HI	TCCGGA	1 cut(s)	131
ApaLI	GTGCAC	1 cut(s)	1498
ApoI	RAATTY	4 cut(s)	2029, 2713, 2921, 2978
Asp700I	GAANNNNTTC	1 cut(s)	1832
AspLEI	GCGC	1 cut(s)	468
AspS9I	GGNCC	7 cut(s)	83, 1175, 1620, 1975, 2352, 2670, 2900
AsuC2I	CCSGG	1 cut(s)	2710
AsuHPI	GGTGA	4 cut(s)	926, 1151, 2165, 3169
AsuII	TTCGAA	1 cut(s)	997
AsuNHI	GCTAGC	1 cut(s)	3211
AvaII	GGWCC	3 cut(s)	1620, 2670, 2900
AxyI	CCTNAGG	1 cut(s)	428
BaeGI	GKGCMC	5 cut(s)	53, 427, 669, 1279, 1502
BalI	TGGCCA	3 cut(s)	5, 1800, 2855
BanI	GGYRCC	1 cut(s)	422
BanII	GRGCYC	2 cut(s)	1794, 2235
BauI	CACGAG	1 cut(s)	2531
BbsI	GAAGAC	2 cut(s)	767, 1546
Bbv12I	GWGCWC	5 cut(s)	78, 321, 1226, 1502, 1794
BccI	CCATC	8 cut(s)	74, 240, 366, 1679, 2116, 2547, 2862, 3106
BceAI	ACGGC	1 cut(s)	3279
BciT130I	CCWGG	5 cut(s)	1005, 1763, 1802, 2646, 3280
BciVI	GTATCC	2 cut(s)	691, 752
BcnI	CCSGG	1 cut(s)	2710
BcoDI	GTCTC	8 cut(s)	14, 133, 385, 592, 969, 1505, 2490, 3287
BfaI	CTAG	3 cut(s)	1892, 2933, 3212
BfmI	CTRYAG	6 cut(s)	1330, 1364, 1820, 2436, 2703, 3469
BfrI	CTTAAG	2 cut(s)	2773, 2945
BfuAI	ACCTGC	1 cut(s)	1371
BfuI	GTATCC	2 cut(s)	691, 752
BglII	AGATCT	3 cut(s)	2175, 2566, 3504
Bme1390I	CCNGG	6 cut(s)	1005, 1763, 1802, 2646, 2710, 3280
Bme18I	GGWCC	3 cut(s)	1620, 2670, 2900
BmgBI	CACGTC	1 cut(s)	2476
BmgT120I	GGNCC	7 cut(s)	83, 1175, 1620, 1975, 2352, 2670, 2900
BmiI	GGNNCC	5 cut(s)	424, 1622, 2234, 2672, 2901
BmrFI	CCNGG	6 cut(s)	1005, 1763, 1802, 2646, 2710, 3280
BmrI	ACTGGG	1 cut(s)	2276
BmtI	GCTAGC	1 cut(s)	3215
BmuI	ACTGGG	1 cut(s)	2276
BpiI	GAAGAC	2 cut(s)	767, 1546
BpmI	CTGGAG	1 cut(s)	612
Bpu10I	CCTNAGC	1 cut(s)	3489
Bpu14I	TTCGAA	1 cut(s)	997
BpuEI	CTTGAG	2 cut(s)	490, 3226
BpuMI	CCSGG	1 cut(s)	2710
Bsa29I	ATCGAT	1 cut(s)	1960
BsaAI	YACGTR	4 cut(s)	92, 2814, 2822, 3427
BsaHI	GRCGYC	1 cut(s)	96
BsaI	GGTCTC	1 cut(s)	3287
BsaJI	CCNNGG	9 cut(s)	245, 729, 1929, 2227, 2292, 2850, 2971, 3004, 3439
BsaWI	WCCGGW	2 cut(s)	131, 3145
BsaXI	ACNNNNNCTCC	2 cut(s)	2262, 2292
Bse1I	ACTGG	7 cut(s)	6, 328, 775, 1321, 1958, 2068, 2271
Bse21I	CCTNAGG	1 cut(s)	428
Bse3DI	GCAATG	3 cut(s)	1440, 1669, 2733
BseAI	TCCGGA	1 cut(s)	131
BseBI	CCWGG	5 cut(s)	1005, 1763, 1802, 2646, 3280
BseCI	ATCGAT	1 cut(s)	1960
BseDI	CCNNGG	9 cut(s)	245, 729, 1929, 2227, 2292, 2850, 2971, 3004, 3439
BseMI	GCAATG	3 cut(s)	1440, 1669, 2733
BseMII	CTCAG	6 cut(s)	128, 396, 1779, 2574, 2919, 3480
BseNI	ACTGG	7 cut(s)	6, 328, 775, 1321, 1958, 2068, 2271
BseRI	GAGGAG	2 cut(s)	100, 2558
BseSI	GKGCMC	5 cut(s)	53, 427, 669, 1279, 1502
BseYI	CCCAGC	6 cut(s)	448, 848, 1177, 2749, 3302, 3375
BsgI	GTGCAG	2 cut(s)	1169, 2526
Bsh1285I	CGRYCG	1 cut(s)	604
BshNI	GGYRCC	1 cut(s)	422
BshVI	ATCGAT	1 cut(s)	1960
BsiEI	CGRYCG	1 cut(s)	604
BsiHKAI	GWGCWC	5 cut(s)	78, 321, 1226, 1502, 1794
BsiSI	CCGG	3 cut(s)	132, 2709, 3146
BslFI	GGGAC	2 cut(s)	283, 2656
BsmAI	GTCTC	8 cut(s)	14, 133, 385, 592, 969, 1505, 2490, 3287
BsmBI	CGTCTC	2 cut(s)	133, 592
BsmFI	GGGAC	2 cut(s)	283, 2656
BsmI	GAATGC	2 cut(s)	487, 2191
Bso31I	GGTCTC	1 cut(s)	3287
Bsp119I	TTCGAA	1 cut(s)	997
Bsp13I	TCCGGA	1 cut(s)	131
Bsp19I	CCATGG	1 cut(s)	2227
BspACI	CCGC	8 cut(s)	18, 119, 622, 912, 949, 2189, 2340, 2579
BspCNI	CTCAG	6 cut(s)	127, 395, 1780, 2575, 2920, 3481
BspDI	ATCGAT	1 cut(s)	1960
BspEI	TCCGGA	1 cut(s)	131
BspHI	TCATGA	3 cut(s)	235, 535, 2367
BspLI	GGNNCC	5 cut(s)	424, 1622, 2234, 2672, 2901
BspMAI	CTGCAG	4 cut(s)	1334, 1368, 2707, 3473
BspMI	ACCTGC	1 cut(s)	1371
BspOI	GCTAGC	1 cut(s)	3215
BspPI	GGATC	9 cut(s)	19, 378, 402, 1686, 2565, 2762, 3137, 3190, 3544
BspQI	GCTCTTC	1 cut(s)	1854
BspT104I	TTCGAA	1 cut(s)	997
BspT107I	GGYRCC	1 cut(s)	422
BspTI	CTTAAG	2 cut(s)	2773, 2945
BspTNI	GGTCTC	1 cut(s)	3287
BsrDI	GCAATG	3 cut(s)	1440, 1669, 2733
BsrI	ACTGG	7 cut(s)	6, 328, 775, 1321, 1958, 2068, 2271
BssECI	CCNNGG	9 cut(s)	245, 729, 1929, 2227, 2292, 2850, 2971, 3004, 3439
BssNI	GRCGYC	1 cut(s)	96
BssSI	CACGAG	1 cut(s)	2531
BssT1I	CCWWGG	5 cut(s)	1929, 2227, 2292, 2971, 3004
Bst2BI	CACGAG	1 cut(s)	2531
Bst2UI	CCWGG	5 cut(s)	1005, 1763, 1802, 2646, 3280
Bst6I	CTCTTC	5 cut(s)	1265, 1854, 2415, 2424, 2931
BstACI	GRCGYC	1 cut(s)	96
BstAFI	CTTAAG	2 cut(s)	2773, 2945
BstAPI	GCANNNNNTGC	1 cut(s)	3326
BstBAI	YACGTR	4 cut(s)	92, 2814, 2822, 3427
BstBI	TTCGAA	1 cut(s)	997
BstC8I	GCNNGC	7 cut(s)	1195, 1996, 2608, 2744, 3165, 3213, 3345
BstDEI	CTNAG	9 cut(s)	114, 382, 428, 659, 1451, 1788, 2583, 2928, 3489
BstDSI	CCRYGG	4 cut(s)	245, 2227, 2850, 3439
BstENI	CCTNNNNNAGG	2 cut(s)	1982, 2359
BstHHI	GCGC	1 cut(s)	468
BstMAI	GTCTC	8 cut(s)	14, 133, 385, 592, 969, 1505, 2490, 3287
BstMCI	CGRYCG	1 cut(s)	604
BstMWI	GCNNNNNNNGC	7 cut(s)	1811, 2441, 2612, 2699, 3275, 3326, 3468
BstNI	CCWGG	5 cut(s)	1005, 1763, 1802, 2646, 3280
BstNSI	RCATGY	1 cut(s)	2702
BstSCI	CCNGG	6 cut(s)	1003, 1761, 1800, 2644, 2708, 3278
BstSFI	CTRYAG	6 cut(s)	1330, 1364, 1820, 2436, 2703, 3469
BstSLI	GKGCMC	5 cut(s)	53, 427, 669, 1279, 1502
BstSNI	TACGTA	1 cut(s)	2814
BstV2I	GAAGAC	2 cut(s)	767, 1546
BstX2I	RGATCY	6 cut(s)	370, 2175, 2557, 2566, 3195, 3504
BstYI	RGATCY	6 cut(s)	370, 2175, 2557, 2566, 3195, 3504
Bsu15I	ATCGAT	1 cut(s)	1960
Bsu36I	CCTNAGG	1 cut(s)	428
BsuI	GTATCC	2 cut(s)	691, 752
BsuTUI	ATCGAT	1 cut(s)	1960
BtgI	CCRYGG	4 cut(s)	245, 2227, 2850, 3439
BtrI	CACGTC	1 cut(s)	2476
BtsI	GCAGTG	4 cut(s)	1215, 2514, 3181, 3534
BtsIMutI	CAGTG	8 cut(s)	321, 1215, 1502, 1951, 2509, 2514, 3181, 3534
BveI	ACCTGC	1 cut(s)	1371
Cac8I	GCNNGC	7 cut(s)	1195, 1996, 2608, 2744, 3165, 3213, 3345
CaiI	CAGNNNCTG	3 cut(s)	2444, 2840, 3163
CciI	TCATGA	3 cut(s)	235, 535, 2367
CfoI	GCGC	1 cut(s)	468
Cfr13I	GGNCC	7 cut(s)	83, 1175, 1620, 1975, 2352, 2670, 2900
ClaI	ATCGAT	1 cut(s)	1960
CseI	GACGC	2 cut(s)	85, 881
CsiI	ACCWGGT	1 cut(s)	1761
Csp6I	GTAC	5 cut(s)	89, 241, 925, 3466, 3510
CviQI	GTAC	5 cut(s)	89, 241, 925, 3466, 3510
DdeI	CTNAG	9 cut(s)	114, 382, 428, 659, 1451, 1788, 2583, 2928, 3489
DraI	TTTAAA	2 cut(s)	877, 2920
DrdI	GACNNNNNNGTC	1 cut(s)	1550
DseDI	GACNNNNNNGTC	1 cut(s)	1550
EaeI	YGGCCR	3 cut(s)	3, 1798, 2853
Eam1104I	CTCTTC	5 cut(s)	1265, 1854, 2415, 2424, 2931
EarI	CTCTTC	5 cut(s)	1265, 1854, 2415, 2424, 2931
EciI	GGCGGA	2 cut(s)	637, 2568
Ecl136II	GAGCTC	1 cut(s)	1792
Eco105I	TACGTA	1 cut(s)	2814
Eco130I	CCWWGG	5 cut(s)	1929, 2227, 2292, 2971, 3004
Eco147I	AGGCCT	1 cut(s)	1003
Eco24I	GRGCYC	2 cut(s)	1794, 2235
Eco31I	GGTCTC	1 cut(s)	3287
Eco32I	GATATC	2 cut(s)	460, 2158
Eco47I	GGWCC	3 cut(s)	1620, 2670, 2900
Eco53kI	GAGCTC	1 cut(s)	1792
Eco57I	CTGAAG	6 cut(s)	761, 1766, 1860, 2124, 3176, 3376
Eco81I	CCTNAGG	1 cut(s)	428
EcoICRI	GAGCTC	1 cut(s)	1792
EcoNI	CCTNNNNNAGG	2 cut(s)	1982, 2359
EcoO109I	RGGNCCY	3 cut(s)	1620, 1975, 2900
EcoRII	CCWGG	5 cut(s)	1003, 1761, 1800, 2644, 3278
EcoRV	GATATC	2 cut(s)	460, 2158
EcoT14I	CCWWGG	5 cut(s)	1929, 2227, 2292, 2971, 3004
EcoT22I	ATGCAT	1 cut(s)	3547
EcoT38I	GRGCYC	2 cut(s)	1794, 2235
ErhI	CCWWGG	5 cut(s)	1929, 2227, 2292, 2971, 3004
Esp3I	CGTCTC	2 cut(s)	133, 592
FalI	AAGNNNNNCTT	6 cut(s)	1724, 1756, 1833, 1865, 2928, 2960
FaqI	GGGAC	2 cut(s)	283, 2656
FauI	CCCGC	1 cut(s)	2182
FriOI	GRGCYC	2 cut(s)	1794, 2235
FspBI	CTAG	3 cut(s)	1892, 2933, 3212
GlaI	GCGC	1 cut(s)	467
GsaI	CCCAGC	6 cut(s)	452, 852, 1181, 2753, 3306, 3379
GsuI	CTGGAG	1 cut(s)	612
HapII	CCGG	3 cut(s)	132, 2709, 3146
HgaI	GACGC	2 cut(s)	85, 881
HhaI	GCGC	1 cut(s)	468
Hin1I	GRCGYC	1 cut(s)	96
Hin6I	GCGC	1 cut(s)	466
HinP1I	GCGC	1 cut(s)	466
HincII	GTYRAC	1 cut(s)	2473
HindII	GTYRAC	1 cut(s)	2473
HindIII	AAGCTT	2 cut(s)	1484, 2942
HpaII	CCGG	3 cut(s)	132, 2709, 3146
HphI	GGTGA	4 cut(s)	926, 1151, 2165, 3169
Hpy166II	GTNNAC	6 cut(s)	189, 652, 1500, 2473, 2479, 3443
Hpy8I	GTNNAC	6 cut(s)	189, 652, 1500, 2473, 2479, 3443
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1811, 2441, 2612, 2699, 3275, 3326, 3468
HpyF3I	CTNAG	9 cut(s)	114, 382, 428, 659, 1451, 1788, 2583, 2928, 3489
Hsp92I	GRCGYC	1 cut(s)	96
HspAI	GCGC	1 cut(s)	466
Kpn2I	TCCGGA	1 cut(s)	131
LguI	GCTCTTC	1 cut(s)	1854
LmnI	GCTCC	7 cut(s)	593, 793, 889, 1532, 2238, 2842, 3160
MabI	ACCWGGT	1 cut(s)	1761
MaeI	CTAG	3 cut(s)	1892, 2933, 3212
MaeIII	GTNAC	2 cut(s)	2059, 2954
MfeI	CAATTG	2 cut(s)	608, 1464
MflI	RGATCY	6 cut(s)	370, 2175, 2557, 2566, 3195, 3504
MlsI	TGGCCA	3 cut(s)	5, 1800, 2855
MluNI	TGGCCA	3 cut(s)	5, 1800, 2855
MlyI	GAGTC	4 cut(s)	481, 756, 1356, 1867
MmeI	TCCRAC	4 cut(s)	1038, 1363, 2348, 3514
Mox20I	TGGCCA	3 cut(s)	5, 1800, 2855
Mph1103I	ATGCAT	1 cut(s)	3547
MroI	TCCGGA	1 cut(s)	131
MroXI	GAANNNNTTC	1 cut(s)	1832
MscI	TGGCCA	3 cut(s)	5, 1800, 2855
MseI	TTAA	9 cut(s)	876, 933, 1242, 2172, 2774, 2919, 2946, 3120, 3135
MslI	CAYNNNNRTG	3 cut(s)	18, 858, 1634
Msp20I	TGGCCA	3 cut(s)	5, 1800, 2855
MspA1I	CMGCKG	5 cut(s)	18, 1181, 2219, 2261, 2749
MspCI	CTTAAG	2 cut(s)	2773, 2945
MspI	CCGG	3 cut(s)	132, 2709, 3146
MspR9I	CCNGG	6 cut(s)	1005, 1763, 1802, 2646, 2710, 3280
MunI	CAATTG	2 cut(s)	608, 1464
Mva1269I	GAATGC	2 cut(s)	487, 2191
MvaI	CCWGG	5 cut(s)	1005, 1763, 1802, 2646, 3280
MwoI	GCNNNNNNNGC	7 cut(s)	1811, 2441, 2612, 2699, 3275, 3326, 3468
NciI	CCSGG	1 cut(s)	2710
NcoI	CCATGG	1 cut(s)	2227
NheI	GCTAGC	1 cut(s)	3211
NlaIV	GGNNCC	5 cut(s)	424, 1622, 2234, 2672, 2901
NmuCI	GTSAC	1 cut(s)	2059
NsiI	ATGCAT	1 cut(s)	3547
NspI	RCATGY	1 cut(s)	2702
NspV	TTCGAA	1 cut(s)	997
OliI	CACNNNNGTG	1 cut(s)	18
PagI	TCATGA	3 cut(s)	235, 535, 2367
PceI	AGGCCT	1 cut(s)	1003
PciSI	GCTCTTC	1 cut(s)	1854
PctI	GAATGC	2 cut(s)	487, 2191
PdmI	GAANNNNTTC	1 cut(s)	1832
PfeI	GAWTC	9 cut(s)	811, 917, 1021, 1167, 1573, 1611, 2485, 2876, 3041
PflFI	GACNNNGTC	1 cut(s)	1346
PflMI	CCANNNNNTGG	1 cut(s)	2645
Ple19I	CGATCG	1 cut(s)	604
PleI	GAGTC	4 cut(s)	480, 755, 1355, 1867
PpsI	GAGTC	4 cut(s)	480, 755, 1355, 1867
Ppu21I	YACGTR	4 cut(s)	92, 2814, 2822, 3427
PpuMI	RGGWCCY	2 cut(s)	1620, 2900
Psp124BI	GAGCTC	1 cut(s)	1794
Psp1406I	AACGTT	1 cut(s)	2251
Psp5II	RGGWCCY	2 cut(s)	1620, 2900
Psp6I	CCWGG	5 cut(s)	1003, 1761, 1800, 2644, 3278
PspFI	CCCAGC	6 cut(s)	448, 848, 1177, 2749, 3302, 3375
PspGI	CCWGG	5 cut(s)	1003, 1761, 1800, 2644, 3278
PspN4I	GGNNCC	5 cut(s)	424, 1622, 2234, 2672, 2901
PspPI	GGNCC	7 cut(s)	83, 1175, 1620, 1975, 2352, 2670, 2900
PspPPI	RGGWCCY	2 cut(s)	1620, 2900
PsrI	GAACNNNNNNTAC	2 cut(s)	672, 704
PstI	CTGCAG	4 cut(s)	1334, 1368, 2707, 3473
PstNI	CAGNNNCTG	3 cut(s)	2444, 2840, 3163
PsuI	RGATCY	6 cut(s)	370, 2175, 2557, 2566, 3195, 3504
PsyI	GACNNNGTC	1 cut(s)	1346
PvuI	CGATCG	1 cut(s)	604
PvuII	CAGCTG	4 cut(s)	1181, 2219, 2261, 2749
RsaI	GTAC	5 cut(s)	90, 242, 926, 3467, 3511
RsaNI	GTAC	5 cut(s)	89, 241, 925, 3466, 3510
RseI	CAYNNNNRTG	3 cut(s)	18, 858, 1634
SacI	GAGCTC	1 cut(s)	1794
SapI	GCTCTTC	1 cut(s)	1854
SaqAI	TTAA	9 cut(s)	876, 933, 1242, 2172, 2774, 2919, 2946, 3120, 3135
Sau96I	GGNCC	7 cut(s)	83, 1175, 1620, 1975, 2352, 2670, 2900
SchI	GAGTC	4 cut(s)	481, 756, 1356, 1867
ScrFI	CCNGG	6 cut(s)	1005, 1763, 1802, 2646, 2710, 3280
SexAI	ACCWGGT	1 cut(s)	1761
SfcI	CTRYAG	6 cut(s)	1330, 1364, 1820, 2436, 2703, 3469
SfuI	TTCGAA	1 cut(s)	997
SinI	GGWCC	3 cut(s)	1620, 2670, 2900
SmiI	ATTTAAAT	1 cut(s)	2920
SmiMI	CAYNNNNRTG	3 cut(s)	18, 858, 1634
SmlI	CTYRAG	4 cut(s)	469, 2773, 2945, 3205
SmoI	CTYRAG	4 cut(s)	469, 2773, 2945, 3205
SnaBI	TACGTA	1 cut(s)	2814
SseBI	AGGCCT	1 cut(s)	1003
SsiI	CCGC	8 cut(s)	18, 119, 622, 912, 949, 2189, 2340, 2579
SspI	AATATT	2 cut(s)	1302, 2379
SspMI	CTAG	3 cut(s)	1892, 2933, 3212
SstI	GAGCTC	1 cut(s)	1794
StuI	AGGCCT	1 cut(s)	1003
StyD4I	CCNGG	6 cut(s)	1003, 1761, 1800, 2644, 2708, 3278
StyI	CCWWGG	5 cut(s)	1929, 2227, 2292, 2971, 3004
SwaI	ATTTAAAT	1 cut(s)	2920
TaqI	TCGA	6 cut(s)	604, 997, 1681, 1960, 2790, 2831
TatI	WGTACW	2 cut(s)	924, 3509
TauI	GCSGC	1 cut(s)	121
TfiI	GAWTC	9 cut(s)	811, 917, 1021, 1167, 1573, 1611, 2485, 2876, 3041
Tru1I	TTAA	9 cut(s)	876, 933, 1242, 2172, 2774, 2919, 2946, 3120, 3135
Tru9I	TTAA	9 cut(s)	876, 933, 1242, 2172, 2774, 2919, 2946, 3120, 3135
TscAI	CASTG	8 cut(s)	328, 1215, 1502, 1958, 2509, 2514, 3188, 3534
TseFI	GTSAC	1 cut(s)	2059
Tsp45I	GTSAC	1 cut(s)	2059
TspGWI	ACGGA	2 cut(s)	781, 1100
TspRI	CASTG	8 cut(s)	328, 1215, 1502, 1958, 2509, 2514, 3188, 3534
Tth111I	GACNNNGTC	1 cut(s)	1346
Van91I	CCANNNNNTGG	1 cut(s)	2645
Vha464I	CTTAAG	2 cut(s)	2773, 2945
VneI	GTGCAC	1 cut(s)	1498
VpaK11BI	GGWCC	3 cut(s)	1620, 2670, 2900
XagI	CCTNNNNNAGG	2 cut(s)	1982, 2359
XapI	RAATTY	4 cut(s)	2029, 2713, 2921, 2978
XceI	RCATGY	1 cut(s)	2702
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1672, 3286
XmnI	GAANNNNTTC	1 cut(s)	1832
XspI	CTAG	3 cut(s)	1892, 2933, 3212
Zsp2I	ATGCAT	1 cut(s)	3547

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.