The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 219, 3155 |
| Acc36I | ACCTGC | 2 cut(s) | 911, 2057 |
| AccB1I | GGYRCC | 1 cut(s) | 273 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2720 |
| AccII | CGCG | 2 cut(s) | 165, 2940 |
| AciI | CCGC | 7 cut(s) | 165, 694, 1998, 2663, 2940, 2978, 3358 |
| AclWI | GGATC | 8 cut(s) | 64, 77, 91, 1017, 1174, 1758, 2740, 2753 |
| AcoI | YGGCCR | 1 cut(s) | 2263 |
| AcsI | RAATTY | 5 cut(s) | 846, 1318, 2101, 2788, 3309 |
| AcuI | CTGAAG | 6 cut(s) | 1110, 2277, 2625, 3081, 3215, 3606 |
| AcyI | GRCGYC | 1 cut(s) | 1072 |
| AfaI | GTAC | 9 cut(s) | 292, 314, 376, 1573, 2376, 3225, 3504, 3542, 3586 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 106, 164, 484, 823, 1915, 2662, 2720, 3573 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1420 |
| AjnI | CCWGG | 5 cut(s) | 1075, 2232, 2364, 3353, 3404 |
| AleI | CACNNNNGTG | 1 cut(s) | 90 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2334, 2366 |
| Alw21I | GWGCWC | 2 cut(s) | 1566, 1859 |
| Alw26I | GTCTC | 6 cut(s) | 55, 664, 1577, 1667, 2845, 3535 |
| Alw44I | GTGCAC | 1 cut(s) | 1562 |
| AlwI | GGATC | 8 cut(s) | 64, 77, 91, 1017, 1174, 1758, 2740, 2753 |
| AoxI | GGCC | 7 cut(s) | 155, 397, 1959, 2263, 2302, 2423, 2974 |
| ApaI | GGGCCC | 1 cut(s) | 159 |
| ApaLI | GTGCAC | 1 cut(s) | 1562 |
| ApoI | RAATTY | 5 cut(s) | 846, 1318, 2101, 2788, 3309 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1904, 2349 |
| AspLEI | GCGC | 3 cut(s) | 668, 2808, 2940 |
| AspS9I | GGNCC | 7 cut(s) | 155, 156, 495, 1494, 1960, 2632, 2975 |
| AsuHPI | GGTGA | 8 cut(s) | 296, 887, 998, 1223, 1469, 2429, 2528, 2537 |
| AsuNHI | GCTAGC | 1 cut(s) | 1547 |
| AvaII | GGWCC | 3 cut(s) | 495, 1494, 2632 |
| BaeGI | GKGCMC | 4 cut(s) | 159, 276, 741, 1566 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 796, 829, 3486, 3519 |
| BalI | TGGCCA | 1 cut(s) | 2265 |
| BamHI | GGATCC | 2 cut(s) | 69, 2745 |
| BanI | GGYRCC | 1 cut(s) | 273 |
| BanII | GRGCYC | 2 cut(s) | 159, 1859 |
| BbsI | GAAGAC | 5 cut(s) | 74, 447, 515, 810, 2099 |
| Bbv12I | GWGCWC | 2 cut(s) | 1566, 1859 |
| BbvCI | CCTCAGC | 1 cut(s) | 2175 |
| BccI | CCATC | 9 cut(s) | 146, 438, 645, 1745, 1751, 2507, 2546, 2622, 3389 |
| BceAI | ACGGC | 2 cut(s) | 2339, 2915 |
| BciT130I | CCWGG | 5 cut(s) | 1077, 2234, 2366, 3355, 3406 |
| BciVI | GTATCC | 2 cut(s) | 526, 824 |
| BcoDI | GTCTC | 6 cut(s) | 55, 664, 1577, 1667, 2845, 3535 |
| BfaI | CTAG | 4 cut(s) | 1548, 1697, 1964, 2222 |
| BfmI | CTRYAG | 6 cut(s) | 522, 918, 2001, 2487, 3339, 3544 |
| BfuAI | ACCTGC | 2 cut(s) | 911, 2057 |
| BfuI | GTATCC | 2 cut(s) | 526, 824 |
| BglII | AGATCT | 3 cut(s) | 559, 2247, 2753 |
| BlpI | GCTNAGC | 1 cut(s) | 1523 |
| Bme1390I | CCNGG | 5 cut(s) | 1077, 2234, 2366, 3355, 3406 |
| Bme18I | GGWCC | 3 cut(s) | 495, 1494, 2632 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1420 |
| BmgT120I | GGNCC | 7 cut(s) | 155, 156, 495, 1494, 1960, 2632, 2975 |
| BmiI | GGNNCC | 8 cut(s) | 71, 157, 275, 496, 497, 1907, 2633, 2747 |
| BmrFI | CCNGG | 5 cut(s) | 1077, 2234, 2366, 3355, 3406 |
| BmrI | ACTGGG | 1 cut(s) | 2530 |
| BmtI | GCTAGC | 1 cut(s) | 1551 |
| BmuI | ACTGGG | 1 cut(s) | 2530 |
| BpiI | GAAGAC | 5 cut(s) | 74, 447, 515, 810, 2099 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2743, 2775 |
| BpmI | CTGGAG | 3 cut(s) | 225, 1421, 2898 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1005, 2175 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1523 |
| BpuEI | CTTGAG | 2 cut(s) | 485, 2869 |
| Bsa29I | ATCGAT | 1 cut(s) | 136 |
| BsaAI | YACGTR | 4 cut(s) | 374, 2897, 3227, 3280 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2744 |
| BsaHI | GRCGYC | 1 cut(s) | 1072 |
| BsaI | GGTCTC | 2 cut(s) | 55, 2845 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2334, 2364 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 106, 164, 484, 823, 1915, 2662, 2720, 3573 |
| Bse3DI | GCAATG | 4 cut(s) | 1884, 2068, 2233, 2808 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2744 |
| BseBI | CCWGG | 5 cut(s) | 1077, 2234, 2366, 3355, 3406 |
| BseCI | ATCGAT | 1 cut(s) | 136 |
| BseJI | GATNNNNATC | 1 cut(s) | 2744 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 106, 164, 484, 823, 1915, 2662, 2720, 3573 |
| BseMI | GCAATG | 4 cut(s) | 1884, 2068, 2233, 2808 |
| BseMII | CTCAG | 9 cut(s) | 35, 200, 675, 722, 1164, 1537, 2166, 2325, 3017 |
| BseRI | GAGGAG | 1 cut(s) | 2543 |
| BseSI | GKGCMC | 4 cut(s) | 159, 276, 741, 1566 |
| BseYI | CCCAGC | 2 cut(s) | 1608, 3047 |
| BsgI | GTGCAG | 1 cut(s) | 317 |
| Bsh1236I | CGCG | 2 cut(s) | 165, 2940 |
| BshFI | GGCC | 7 cut(s) | 157, 399, 1961, 2265, 2304, 2425, 2976 |
| BshNI | GGYRCC | 1 cut(s) | 273 |
| BshVI | ATCGAT | 1 cut(s) | 136 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1566, 1859 |
| BsiSI | CCGG | 1 cut(s) | 1383 |
| BslFI | GGGAC | 7 cut(s) | 54, 122, 136, 481, 839, 1061, 2645 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 106, 164, 484, 823, 1915, 2662, 2720, 3573 |
| BsmAI | GTCTC | 6 cut(s) | 55, 664, 1577, 1667, 2845, 3535 |
| BsmFI | GGGAC | 7 cut(s) | 54, 122, 136, 481, 839, 1061, 2645 |
| BsmI | GAATGC | 1 cut(s) | 3084 |
| BsnI | GGCC | 7 cut(s) | 157, 399, 1961, 2265, 2304, 2425, 2976 |
| Bso31I | GGTCTC | 2 cut(s) | 55, 2845 |
| Bsp120I | GGGCCC | 1 cut(s) | 155 |
| Bsp1286I | GDGCHC | 5 cut(s) | 159, 276, 741, 1566, 1859 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1523 |
| Bsp19I | CCATGG | 1 cut(s) | 317 |
| BspACI | CCGC | 7 cut(s) | 165, 694, 1998, 2663, 2940, 2978, 3358 |
| BspANI | GGCC | 7 cut(s) | 157, 399, 1961, 2265, 2304, 2425, 2976 |
| BspCNI | CTCAG | 9 cut(s) | 36, 199, 674, 723, 1163, 1536, 2167, 2326, 3016 |
| BspDI | ATCGAT | 1 cut(s) | 136 |
| BspFNI | CGCG | 2 cut(s) | 165, 2940 |
| BspHI | TCATGA | 3 cut(s) | 925, 1579, 2439 |
| BspLI | GGNNCC | 8 cut(s) | 71, 157, 275, 496, 497, 1907, 2633, 2747 |
| BspMAI | CTGCAG | 4 cut(s) | 526, 922, 2491, 3343 |
| BspMI | ACCTGC | 2 cut(s) | 911, 2057 |
| BspOI | GCTAGC | 1 cut(s) | 1551 |
| BspPI | GGATC | 8 cut(s) | 64, 77, 91, 1017, 1174, 1758, 2740, 2753 |
| BspQI | GCTCTTC | 2 cut(s) | 2055, 3080 |
| BspT107I | GGYRCC | 1 cut(s) | 273 |
| BspTNI | GGTCTC | 2 cut(s) | 55, 2845 |
| BsrDI | GCAATG | 4 cut(s) | 1884, 2068, 2233, 2808 |
| BssNI | GRCGYC | 1 cut(s) | 1072 |
| BssT1I | CCWWGG | 4 cut(s) | 317, 1191, 2672, 3079 |
| Bst2UI | CCWGG | 5 cut(s) | 1077, 2234, 2366, 3355, 3406 |
| Bst6I | CTCTTC | 9 cut(s) | 17, 1581, 1817, 2055, 2433, 2490, 3006, 3080, 3191 |
| BstACI | GRCGYC | 1 cut(s) | 1072 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1883, 3415 |
| BstBAI | YACGTR | 4 cut(s) | 374, 2897, 3227, 3280 |
| BstC8I | GCNNGC | 9 cut(s) | 1549, 1875, 2558, 2726, 2810, 2978, 2982, 3240, 3288 |
| BstDSI | CCRYGG | 2 cut(s) | 65, 317 |
| BstFNI | CGCG | 2 cut(s) | 165, 2940 |
| BstHHI | GCGC | 3 cut(s) | 668, 2808, 2940 |
| BstMAI | GTCTC | 6 cut(s) | 55, 664, 1577, 1667, 2845, 3535 |
| BstNI | CCWGG | 5 cut(s) | 1077, 2234, 2366, 3355, 3406 |
| BstNSI | RCATGY | 2 cut(s) | 2728, 2777 |
| BstSCI | CCNGG | 5 cut(s) | 1075, 2232, 2364, 3353, 3404 |
| BstSFI | CTRYAG | 6 cut(s) | 522, 918, 2001, 2487, 3339, 3544 |
| BstSLI | GKGCMC | 4 cut(s) | 159, 276, 741, 1566 |
| BstUI | CGCG | 2 cut(s) | 165, 2940 |
| BstV2I | GAAGAC | 5 cut(s) | 74, 447, 515, 810, 2099 |
| BstX2I | RGATCY | 6 cut(s) | 69, 559, 1166, 2247, 2745, 2753 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1504 |
| BstYI | RGATCY | 6 cut(s) | 69, 559, 1166, 2247, 2745, 2753 |
| Bsu15I | ATCGAT | 1 cut(s) | 136 |
| BsuI | GTATCC | 2 cut(s) | 526, 824 |
| BsuRI | GGCC | 7 cut(s) | 157, 399, 1961, 2265, 2304, 2425, 2976 |
| BsuTUI | ATCGAT | 1 cut(s) | 136 |
| BtgI | CCRYGG | 2 cut(s) | 65, 317 |
| BtsI | GCAGTG | 6 cut(s) | 984, 1995, 2574, 3256, 3336, 3609 |
| BveI | ACCTGC | 2 cut(s) | 911, 2057 |
| Cac8I | GCNNGC | 9 cut(s) | 1549, 1875, 2558, 2726, 2810, 2978, 2982, 3240, 3288 |
| CciI | TCATGA | 3 cut(s) | 925, 1579, 2439 |
| CfoI | GCGC | 3 cut(s) | 668, 2808, 2940 |
| Cfr13I | GGNCC | 7 cut(s) | 155, 156, 495, 1494, 1960, 2632, 2975 |
| ClaI | ATCGAT | 1 cut(s) | 136 |
| CseI | GACGC | 1 cut(s) | 1080 |
| Csp6I | GTAC | 9 cut(s) | 291, 313, 375, 1572, 2375, 3224, 3503, 3541, 3585 |
| CviQI | GTAC | 9 cut(s) | 291, 313, 375, 1572, 2375, 3224, 3503, 3541, 3585 |
| DraI | TTTAAA | 1 cut(s) | 1092 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1420 |
| EaeI | YGGCCR | 1 cut(s) | 2263 |
| Eam1104I | CTCTTC | 9 cut(s) | 17, 1581, 1817, 2055, 2433, 2490, 3006, 3080, 3191 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1420 |
| EarI | CTCTTC | 9 cut(s) | 17, 1581, 1817, 2055, 2433, 2490, 3006, 3080, 3191 |
| EciI | GGCGGA | 1 cut(s) | 709 |
| Ecl136II | GAGCTC | 1 cut(s) | 1857 |
| Eco130I | CCWWGG | 4 cut(s) | 317, 1191, 2672, 3079 |
| Eco24I | GRGCYC | 2 cut(s) | 159, 1859 |
| Eco31I | GGTCTC | 2 cut(s) | 55, 2845 |
| Eco32I | GATATC | 1 cut(s) | 2922 |
| Eco47I | GGWCC | 3 cut(s) | 495, 1494, 2632 |
| Eco53kI | GAGCTC | 1 cut(s) | 1857 |
| Eco57I | CTGAAG | 6 cut(s) | 1110, 2277, 2625, 3081, 3215, 3606 |
| EcoICRI | GAGCTC | 1 cut(s) | 1857 |
| EcoO109I | RGGNCCY | 2 cut(s) | 495, 2632 |
| EcoRI | GAATTC | 1 cut(s) | 846 |
| EcoRII | CCWGG | 5 cut(s) | 1075, 2232, 2364, 3353, 3404 |
| EcoRV | GATATC | 1 cut(s) | 2922 |
| EcoT14I | CCWWGG | 4 cut(s) | 317, 1191, 2672, 3079 |
| EcoT22I | ATGCAT | 1 cut(s) | 249 |
| EcoT38I | GRGCYC | 2 cut(s) | 159, 1859 |
| ErhI | CCWWGG | 4 cut(s) | 317, 1191, 2672, 3079 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 429, 461, 581, 613, 998, 1030 |
| FaqI | GGGAC | 7 cut(s) | 54, 122, 136, 481, 839, 1061, 2645 |
| FauI | CCCGC | 2 cut(s) | 2933, 2985 |
| FauNDI | CATATG | 1 cut(s) | 3499 |
| FriOI | GRGCYC | 2 cut(s) | 159, 1859 |
| FspBI | CTAG | 4 cut(s) | 1548, 1697, 1964, 2222 |
| GlaI | GCGC | 3 cut(s) | 667, 2807, 2939 |
| GsaI | CCCAGC | 2 cut(s) | 1612, 3051 |
| GsuI | CTGGAG | 3 cut(s) | 225, 1421, 2898 |
| HaeIII | GGCC | 7 cut(s) | 157, 399, 1961, 2265, 2304, 2425, 2976 |
| HapII | CCGG | 1 cut(s) | 1383 |
| HgaI | GACGC | 1 cut(s) | 1080 |
| HhaI | GCGC | 3 cut(s) | 668, 2808, 2940 |
| Hin1I | GRCGYC | 1 cut(s) | 1072 |
| Hin6I | GCGC | 3 cut(s) | 666, 2806, 2938 |
| HinP1I | GCGC | 3 cut(s) | 666, 2806, 2938 |
| HincII | GTYRAC | 2 cut(s) | 6, 3057 |
| HindII | GTYRAC | 2 cut(s) | 6, 3057 |
| HpaI | GTTAAC | 1 cut(s) | 3057 |
| HpaII | CCGG | 1 cut(s) | 1383 |
| HphI | GGTGA | 8 cut(s) | 296, 887, 998, 1223, 1469, 2429, 2528, 2537 |
| Hpy166II | GTNNAC | 7 cut(s) | 6, 172, 724, 1564, 1572, 3057, 3230 |
| Hpy8I | GTNNAC | 7 cut(s) | 6, 172, 724, 1564, 1572, 3057, 3230 |
| Hpy99I | CGWCG | 3 cut(s) | 146, 864, 1074 |
| HpyCH4IV | ACGT | 6 cut(s) | 373, 828, 964, 2896, 3226, 3279 |
| HpySE526I | ACGT | 6 cut(s) | 373, 828, 964, 2896, 3226, 3279 |
| Hsp92I | GRCGYC | 1 cut(s) | 1072 |
| HspAI | GCGC | 3 cut(s) | 666, 2806, 2938 |
| KflI | GGGWCCC | 1 cut(s) | 495 |
| KspAI | GTTAAC | 1 cut(s) | 3057 |
| LguI | GCTCTTC | 2 cut(s) | 2055, 3080 |
| LmnI | GCTCC | 9 cut(s) | 1854, 2236, 2530, 2647, 2716, 2848, 3190, 3235, 3422 |
| MaeI | CTAG | 4 cut(s) | 1548, 1697, 1964, 2222 |
| MaeII | ACGT | 6 cut(s) | 373, 828, 964, 2896, 3226, 3279 |
| MaeIII | GTNAC | 8 cut(s) | 369, 829, 1211, 2131, 2516, 2572, 3029, 3481 |
| MfeI | CAATTG | 1 cut(s) | 2007 |
| MflI | RGATCY | 6 cut(s) | 69, 559, 1166, 2247, 2745, 2753 |
| MhlI | GDGCHC | 5 cut(s) | 159, 276, 741, 1566, 1859 |
| MlsI | TGGCCA | 1 cut(s) | 2265 |
| MluNI | TGGCCA | 1 cut(s) | 2265 |
| MlyI | GAGTC | 4 cut(s) | 118, 1102, 1633, 2445 |
| MmeI | TCCRAC | 6 cut(s) | 883, 1222, 2378, 2445, 2738, 2988 |
| Mox20I | TGGCCA | 1 cut(s) | 2265 |
| Mph1103I | ATGCAT | 1 cut(s) | 249 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1904, 2349 |
| MscI | TGGCCA | 1 cut(s) | 2265 |
| MslI | CAYNNNNRTG | 2 cut(s) | 90, 930 |
| Msp20I | TGGCCA | 1 cut(s) | 2265 |
| MspA1I | CMGCKG | 2 cut(s) | 1253, 3047 |
| MspI | CCGG | 1 cut(s) | 1383 |
| MspR9I | CCNGG | 5 cut(s) | 1077, 2234, 2366, 3355, 3406 |
| MunI | CAATTG | 1 cut(s) | 2007 |
| Mva1269I | GAATGC | 1 cut(s) | 3084 |
| MvaI | CCWGG | 5 cut(s) | 1077, 2234, 2366, 3355, 3406 |
| MvnI | CGCG | 2 cut(s) | 165, 2940 |
| NcoI | CCATGG | 1 cut(s) | 317 |
| NdeI | CATATG | 1 cut(s) | 3499 |
| NheI | GCTAGC | 1 cut(s) | 1547 |
| NlaIV | GGNNCC | 8 cut(s) | 71, 157, 275, 496, 497, 1907, 2633, 2747 |
| NmuCI | GTSAC | 5 cut(s) | 369, 1211, 2516, 2572, 3481 |
| NsiI | ATGCAT | 1 cut(s) | 249 |
| NspI | RCATGY | 2 cut(s) | 2728, 2777 |
| OliI | CACNNNNGTG | 1 cut(s) | 90 |
| PaeI | GCATGC | 1 cut(s) | 2728 |
| PagI | TCATGA | 3 cut(s) | 925, 1579, 2439 |
| PasI | CCCWGGG | 1 cut(s) | 2365 |
| PciSI | GCTCTTC | 2 cut(s) | 2055, 3080 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1018 |
| PctI | GAATGC | 1 cut(s) | 3084 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1904, 2349 |
| PfeI | GAWTC | 9 cut(s) | 138, 883, 907, 989, 1950, 2829, 2951, 3116, 3388 |
| PflFI | GACNNNGTC | 1 cut(s) | 543 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2720 |
| PfoI | TCCNGGA | 1 cut(s) | 2232 |
| PleI | GAGTC | 4 cut(s) | 118, 1102, 1633, 2444 |
| PpsI | GAGTC | 4 cut(s) | 118, 1102, 1633, 2444 |
| Ppu21I | YACGTR | 4 cut(s) | 374, 2897, 3227, 3280 |
| PpuMI | RGGWCCY | 2 cut(s) | 495, 2632 |
| PsiI | TTATAA | 2 cut(s) | 219, 3155 |
| Psp124BI | GAGCTC | 1 cut(s) | 1859 |
| Psp5II | RGGWCCY | 2 cut(s) | 495, 2632 |
| Psp6I | CCWGG | 5 cut(s) | 1075, 2232, 2364, 3353, 3404 |
| PspFI | CCCAGC | 2 cut(s) | 1608, 3047 |
| PspGI | CCWGG | 5 cut(s) | 1075, 2232, 2364, 3353, 3404 |
| PspN4I | GGNNCC | 8 cut(s) | 71, 157, 275, 496, 497, 1907, 2633, 2747 |
| PspOMI | GGGCCC | 1 cut(s) | 155 |
| PspPI | GGNCC | 7 cut(s) | 155, 156, 495, 1494, 1960, 2632, 2975 |
| PspPPI | RGGWCCY | 2 cut(s) | 495, 2632 |
| PstI | CTGCAG | 4 cut(s) | 526, 922, 2491, 3343 |
| PsuI | RGATCY | 6 cut(s) | 69, 559, 1166, 2247, 2745, 2753 |
| PsyI | GACNNNGTC | 1 cut(s) | 543 |
| PvuII | CAGCTG | 2 cut(s) | 1253, 3047 |
| RsaI | GTAC | 9 cut(s) | 292, 314, 376, 1573, 2376, 3225, 3504, 3542, 3586 |
| RsaNI | GTAC | 9 cut(s) | 291, 313, 375, 1572, 2375, 3224, 3503, 3541, 3585 |
| RseI | CAYNNNNRTG | 2 cut(s) | 90, 930 |
| SacI | GAGCTC | 1 cut(s) | 1859 |
| SapI | GCTCTTC | 2 cut(s) | 2055, 3080 |
| Sau96I | GGNCC | 7 cut(s) | 155, 156, 495, 1494, 1960, 2632, 2975 |
| SchI | GAGTC | 4 cut(s) | 118, 1102, 1633, 2445 |
| ScrFI | CCNGG | 5 cut(s) | 1077, 2234, 2366, 3355, 3406 |
| SduI | GDGCHC | 5 cut(s) | 159, 276, 741, 1566, 1859 |
| SfcI | CTRYAG | 6 cut(s) | 522, 918, 2001, 2487, 3339, 3544 |
| SinI | GGWCC | 3 cut(s) | 495, 1494, 2632 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 90, 930 |
| SmlI | CTYRAG | 2 cut(s) | 500, 2848 |
| SmoI | CTYRAG | 2 cut(s) | 500, 2848 |
| SphI | GCATGC | 1 cut(s) | 2728 |
| SsiI | CCGC | 7 cut(s) | 165, 694, 1998, 2663, 2940, 2978, 3358 |
| SspI | AATATT | 4 cut(s) | 1374, 1658, 1978, 2473 |
| SspMI | CTAG | 4 cut(s) | 1548, 1697, 1964, 2222 |
| SstI | GAGCTC | 1 cut(s) | 1859 |
| StyD4I | CCNGG | 5 cut(s) | 1075, 2232, 2364, 3353, 3404 |
| StyI | CCWWGG | 4 cut(s) | 317, 1191, 2672, 3079 |
| TaiI | ACGT | 6 cut(s) | 376, 831, 967, 2899, 3229, 3282 |
| TaqI | TCGA | 8 cut(s) | 136, 141, 613, 794, 1069, 1082, 3384, 3652 |
| TatI | WGTACW | 2 cut(s) | 1571, 3584 |
| TauI | GCSGC | 1 cut(s) | 3361 |
| TfiI | GAWTC | 9 cut(s) | 138, 883, 907, 989, 1950, 2829, 2951, 3116, 3388 |
| TseFI | GTSAC | 5 cut(s) | 369, 1211, 2516, 2572, 3481 |
| Tsp45I | GTSAC | 5 cut(s) | 369, 1211, 2516, 2572, 3481 |
| TspGWI | ACGGA | 3 cut(s) | 467, 806, 1580 |
| Tth111I | GACNNNGTC | 1 cut(s) | 543 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2720 |
| VneI | GTGCAC | 1 cut(s) | 1562 |
| VpaK11BI | GGWCC | 3 cut(s) | 495, 1494, 2632 |
| XapI | RAATTY | 5 cut(s) | 846, 1318, 2101, 2788, 3309 |
| XceI | RCATGY | 2 cut(s) | 2728, 2777 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 1744, 1954, 3361 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1904, 2349 |
| XspI | CTAG | 4 cut(s) | 1548, 1697, 1964, 2222 |
| Zsp2I | ATGCAT | 1 cut(s) | 249 |