Rh5AG459900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000764
Physical Location & Seq
Reverse (-)
78835145 .. 78840891
5747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG459900.1

Sequence Viewer

Length: 303 bp
ATGGGCAGGCAAAGCCCACATTGCAAAGCCCAAGTTCCCGTACCAGAGGCTGAACAACGACCCGAGCTTGAGAGTCTTGAACAAGCTGCTCTTCGGCTCGACGGTCTCACCGGAGCTCTCGGCAGTAGCGGCGACCTCGAAACTGCTCCGATCAATGGCAGGAGAAGTCCAGGGCCTGAGCTTGAAATGGGAGGAGGAGAGGAACCAGGGTCGGCGGGAAAGAGTGGCAGACAGGGCGTCATTGGTGAGGAGGTGAGAGCGGTGTCAGGCTTGAGCGAGGCGGAGACACGTGTGTGCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

10.28

Weight (kDa)

4.75

Isoelectric Point (pI)

67.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 260
AciI CCGC 4 cut(s) 129, 215, 260, 281
AcvI CACGTG 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 155
AflIII ACRYGT 2 cut(s) 287, 289
AgsI TTSAA 2 cut(s) 80, 185
AhdI GACNNNNNGTC 1 cut(s) 236
AjnI CCWGG 2 cut(s) 169, 205
AleI CACNNNNGTG 1 cut(s) 292
AluBI AGCT 4 cut(s) 67, 86, 116, 181
AluI AGCT 4 cut(s) 67, 86, 116, 181
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 2 cut(s) 110, 278
AlwNI CAGNNNCTG 2 cut(s) 50, 176
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 1 cut(s) 173
ApeKI GCWGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 3 cut(s) 100, 257, 265
AvaI CYCGRG 1 cut(s) 62
BanII GRGCYC 1 cut(s) 118
BbrPI CACGTG 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 1 cut(s) 73
BciT130I CCWGG 2 cut(s) 171, 207
BcoDI GTCTC 2 cut(s) 110, 278
BisI GCNGC 2 cut(s) 87, 130
BlsI GCNGC 2 cut(s) 88, 131
Bme1390I CCNGG 2 cut(s) 171, 207
BmeRI GACNNNNNGTC 1 cut(s) 236
BmeT110I CYCGRG 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 171, 207
Bpu10I CCTNAGC 1 cut(s) 177
BpuEI CTTGAG 2 cut(s) 89, 292
BsaAI YACGTR 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 2 cut(s) 170, 206
BsaWI WCCGGW 1 cut(s) 110
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse3DI GCAATG 1 cut(s) 19
BseBI CCWGG 2 cut(s) 171, 207
BseDI CCNNGG 2 cut(s) 170, 206
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 1 cut(s) 19
BseMII CTCAG 1 cut(s) 168
BseRI GAGGAG 3 cut(s) 207, 210, 263
BseXI GCAGC 1 cut(s) 73
BshFI GGCC 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 118
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 1 cut(s) 111
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 110, 278
BsnI GGCC 1 cut(s) 175
Bso31I GGTCTC 1 cut(s) 110
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 1 cut(s) 150
BspACI CCGC 4 cut(s) 129, 215, 260, 281
BspANI GGCC 1 cut(s) 175
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 204
BspQI GCTCTTC 1 cut(s) 96
BspTNI GGTCTC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 19
BssECI CCNNGG 2 cut(s) 170, 206
BssMI GATC 1 cut(s) 150
BssNI GRCGYC 1 cut(s) 237
Bst2UI CCWGG 2 cut(s) 171, 207
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 1 cut(s) 96
BstACI GRCGYC 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 8
BstDEI CTNAG 1 cut(s) 177
BstKTI GATC 1 cut(s) 153
BstMAI GTCTC 2 cut(s) 110, 278
BstMBI GATC 1 cut(s) 150
BstMWI GCNNNNNNNGC 4 cut(s) 12, 21, 129, 234
BstNI CCWGG 2 cut(s) 171, 207
BstSCI CCNGG 2 cut(s) 169, 205
BstV1I GCAGC 1 cut(s) 73
BsuRI GGCC 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 8
CaiI CAGNNNCTG 2 cut(s) 50, 176
Cfr13I GGNCC 1 cut(s) 173
CseI GACGC 1 cut(s) 226
Csp6I GTAC 1 cut(s) 41
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 1 cut(s) 177
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
DriI GACNNNNNGTC 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 96
Eam1105I GACNNNNNGTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 96
EciI GGCGGA 1 cut(s) 296
Ecl136II GAGCTC 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 110
Eco53kI GAGCTC 1 cut(s) 116
Eco72I CACGTG 1 cut(s) 290
Eco88I CYCGRG 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 173
EcoRII CCWGG 2 cut(s) 169, 205
EcoT38I GRGCYC 1 cut(s) 118
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FauI CCCGC 1 cut(s) 208
Fnu4HI GCNGC 2 cut(s) 87, 130
FriOI GRGCYC 1 cut(s) 118
Fsp4HI GCNGC 2 cut(s) 87, 130
GluI GCNGC 2 cut(s) 87, 130
HaeIII GGCC 1 cut(s) 175
HapII CCGG 1 cut(s) 111
HgaI GACGC 1 cut(s) 226
Hin1I GRCGYC 1 cut(s) 237
HinfI GANTC 1 cut(s) 73
HpaII CCGG 1 cut(s) 111
HphI GGTGA 3 cut(s) 100, 257, 265
Hpy188I TCNGA 1 cut(s) 150
Hpy188III TCNNGA 1 cut(s) 77
Hpy99I CGWCG 1 cut(s) 104
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 1 cut(s) 24
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 21, 129, 234
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 289
Hsp92I GRCGYC 1 cut(s) 237
Kzo9I GATC 1 cut(s) 150
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 2 cut(s) 113, 151
Lsp1109I GCAGC 1 cut(s) 73
MaeII ACGT 1 cut(s) 289
MalI GATC 1 cut(s) 152
MbiI CCGCTC 1 cut(s) 260
MboI GATC 1 cut(s) 150
MboII GAAGA 1 cut(s) 83
MhlI GDGCHC 1 cut(s) 118
MlyI GAGTC 1 cut(s) 82
MnlI CCTC 8 cut(s) 40, 146, 185, 188, 193, 241, 244, 271
MslI CAYNNNNRTG 1 cut(s) 292
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 171, 207
MvaI CCWGG 2 cut(s) 171, 207
MwoI GCNNNNNNNGC 4 cut(s) 12, 21, 129, 234
NdeII GATC 1 cut(s) 150
NlaIV GGNNCC 1 cut(s) 204
NmeAIII GCCGAG 1 cut(s) 99
OliI CACNNNNGTG 1 cut(s) 292
PciSI GCTCTTC 1 cut(s) 96
PkrI GCNGC 2 cut(s) 88, 131
PleI GAGTC 1 cut(s) 81
PmaCI CACGTG 1 cut(s) 290
PmlI CACGTG 1 cut(s) 290
PpsI GAGTC 1 cut(s) 81
Ppu21I YACGTR 1 cut(s) 290
Psp124BI GAGCTC 1 cut(s) 118
Psp6I CCWGG 2 cut(s) 169, 205
PspCI CACGTG 1 cut(s) 290
PspGI CCWGG 2 cut(s) 169, 205
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 173
PstNI CAGNNNCTG 2 cut(s) 50, 176
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 292
SacI GAGCTC 1 cut(s) 118
SapI GCTCTTC 1 cut(s) 96
SatI GCNGC 2 cut(s) 87, 130
Sau3AI GATC 1 cut(s) 150
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 2 cut(s) 171, 207
SduI GDGCHC 1 cut(s) 118
SetI ASST 7 cut(s) 69, 88, 118, 138, 183, 255, 292
SmiMI CAYNNNNRTG 1 cut(s) 292
SmlI CTYRAG 2 cut(s) 68, 271
SmoI CTYRAG 2 cut(s) 68, 271
SsiI CCGC 4 cut(s) 129, 215, 260, 281
SstI GAGCTC 1 cut(s) 118
StyD4I CCNGG 2 cut(s) 169, 205
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 1 cut(s) 292
TaqI TCGA 2 cut(s) 99, 138
TauI GCSGC 1 cut(s) 132
TseI GCWGC 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.