Rh4CG068200

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000764
Physical Location & Seq
Forward (+)
12740830 .. 12742849
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG068200.1

Sequence Viewer

Length: 468 bp
ATGGTCAAATCGATCAGCTGCTTGGAATTCAATGACTTCCTCCAGCAAAGCGGCTACAACTGGATTATTAACGACCCAAAATTCTTCAAAAGGCTCGACCGCAATGGCGATAATGGATTGGATTTTGGGGAAGCTCTCACATTCTACTACATCATTAAAACGAGGTACATCTTATGCCAAGGGTACCAGTGCGGCGTACACCTTTGCGATGTTGAACAGGTACCAGAGGCCGAACAACGACCCGAGCTCGAGAGTCTTGAACAAGTTGCTCTTAGGCTCGGCGGTCTTGTCGGAGCTCTCGGCAGTAGCAGCGACCTCAAAACTGGTCCGACCGACGACAGGAGAAGTCTAGGGCCTGAGCTTGAAATCGGAGGAGGAGAGGGACCAGGATCGGCGGGAAAGAGCGGCGGAGAGGAGGAGGTTTGGGCGGAGAGGCGGAGGTTTGGGCGGAGAGGCGGTGAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

155

Amino Acids

17.12

Weight (kDa)

4.83

Isoelectric Point (pI)

54.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 183, 220
AccB1I GGYRCC 2 cut(s) 183, 220
AccBSI CCGCTC 1 cut(s) 405
AclWI GGATC 1 cut(s) 397
AcsI RAATTY 2 cut(s) 26, 80
AfaI GTAC 4 cut(s) 167, 185, 198, 222
AfiI CCNNNNNNNGG 1 cut(s) 339
AgsI TTSAA 5 cut(s) 31, 88, 215, 260, 365
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 5 cut(s) 18, 134, 247, 296, 361
AluI AGCT 5 cut(s) 18, 134, 247, 296, 361
Alw21I GWGCWC 2 cut(s) 249, 298
Alw26I GTCTC 1 cut(s) 455
AlwI GGATC 1 cut(s) 397
Ama87I CYCGRG 2 cut(s) 242, 248
AoxI GGCC 2 cut(s) 228, 353
ApeKI GCWGC 2 cut(s) 18, 309
ApoI RAATTY 2 cut(s) 26, 80
Asp718I GGTACC 2 cut(s) 183, 220
AspS9I GGNCC 3 cut(s) 326, 353, 383
AvaI CYCGRG 2 cut(s) 242, 248
AvaII GGWCC 2 cut(s) 326, 383
BanI GGYRCC 2 cut(s) 183, 220
BanII GRGCYC 2 cut(s) 249, 298
Bbv12I GWGCWC 2 cut(s) 249, 298
BbvI GCAGC 2 cut(s) 5, 321
BciT130I CCWGG 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 1 cut(s) 350
BisI GCNGC 5 cut(s) 19, 52, 193, 310, 406
BlsI GCNGC 5 cut(s) 20, 53, 194, 311, 407
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 2 cut(s) 326, 383
BmeT110I CYCGRG 2 cut(s) 242, 248
BmgT120I GGNCC 3 cut(s) 326, 353, 383
BmiI GGNNCC 3 cut(s) 185, 222, 384
BmrFI CCNGG 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 357
Bsa29I ATCGAT 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 178
BsaXI ACNNNNNCTCC 1 cut(s) 442
Bsc4I CCNNNNNNNGG 1 cut(s) 339
Bse1I ACTGG 3 cut(s) 65, 187, 328
Bse3DI GCAATG 1 cut(s) 109
BseBI CCWGG 1 cut(s) 387
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 339
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 3 cut(s) 65, 187, 328
BseRI GAGGAG 4 cut(s) 387, 390, 428, 431
BseXI GCAGC 2 cut(s) 5, 321
Bsh1285I CGRYCG 2 cut(s) 100, 333
BshFI GGCC 2 cut(s) 230, 355
BshNI GGYRCC 2 cut(s) 183, 220
BshVI ATCGAT 1 cut(s) 11
BsiEI CGRYCG 2 cut(s) 100, 333
BsiHKAI GWGCWC 2 cut(s) 249, 298
BsiHKCI CYCGRG 2 cut(s) 242, 248
BslFI GGGAC 1 cut(s) 396
BslI CCNNNNNNNGG 1 cut(s) 339
BsmAI GTCTC 1 cut(s) 455
BsmFI GGGAC 1 cut(s) 396
BsnI GGCC 2 cut(s) 230, 355
BsoBI CYCGRG 2 cut(s) 242, 248
Bsp1286I GDGCHC 2 cut(s) 249, 298
Bsp143I GATC 2 cut(s) 12, 389
BspANI GGCC 2 cut(s) 230, 355
BspCNI CTCAG 1 cut(s) 349
BspDI ATCGAT 1 cut(s) 11
BspLI GGNNCC 3 cut(s) 185, 222, 384
BspPI GGATC 1 cut(s) 397
BspT107I GGYRCC 2 cut(s) 183, 220
BsrBI CCGCTC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 109
BsrI ACTGG 3 cut(s) 65, 187, 328
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 2 cut(s) 12, 389
BssT1I CCWWGG 1 cut(s) 178
Bst2UI CCWGG 1 cut(s) 387
BstDEI CTNAG 2 cut(s) 272, 357
BstKTI GATC 2 cut(s) 15, 392
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 2 cut(s) 12, 389
BstMCI CGRYCG 2 cut(s) 100, 333
BstMWI GCNNNNNNNGC 1 cut(s) 309
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 5, 321
Bsu15I ATCGAT 1 cut(s) 11
BsuRI GGCC 2 cut(s) 230, 355
BsuTUI ATCGAT 1 cut(s) 11
BtgZI GCGATG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 194
Cfr13I GGNCC 3 cut(s) 326, 353, 383
ClaI ATCGAT 1 cut(s) 11
Csp6I GTAC 4 cut(s) 166, 184, 197, 221
CviQI GTAC 4 cut(s) 166, 184, 197, 221
DdeI CTNAG 2 cut(s) 272, 357
DpnI GATC 2 cut(s) 14, 391
DpnII GATC 2 cut(s) 12, 389
EciI GGCGGA 4 cut(s) 423, 443, 451, 463
Ecl136II GAGCTC 2 cut(s) 247, 296
Eco130I CCWWGG 1 cut(s) 178
Eco24I GRGCYC 2 cut(s) 249, 298
Eco47I GGWCC 2 cut(s) 326, 383
Eco53kI GAGCTC 2 cut(s) 247, 296
Eco88I CYCGRG 2 cut(s) 242, 248
EcoICRI GAGCTC 2 cut(s) 247, 296
EcoO109I RGGNCCY 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 178
EcoT38I GRGCYC 2 cut(s) 249, 298
ErhI CCWWGG 1 cut(s) 178
FaiI YATR 1 cut(s) 175
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FaqI GGGAC 1 cut(s) 396
FauI CCCGC 1 cut(s) 388
Fnu4HI GCNGC 5 cut(s) 19, 52, 193, 310, 406
FriOI GRGCYC 2 cut(s) 249, 298
Fsp4HI GCNGC 5 cut(s) 19, 52, 193, 310, 406
FspBI CTAG 1 cut(s) 350
GluI GCNGC 5 cut(s) 19, 52, 193, 310, 406
GsuI CTGGAG 1 cut(s) 26
HaeIII GGCC 2 cut(s) 230, 355
HinfI GANTC 1 cut(s) 253
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 3 cut(s) 293, 330, 371
Hpy188III TCNNGA 2 cut(s) 250, 257
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 1 cut(s) 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 309
HpyF3I CTNAG 2 cut(s) 272, 357
KpnI GGTACC 2 cut(s) 187, 224
Kzo9I GATC 2 cut(s) 12, 389
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 2 cut(s) 5, 321
MaeI CTAG 1 cut(s) 350
MalI GATC 2 cut(s) 14, 391
MbiI CCGCTC 1 cut(s) 405
MboI GATC 2 cut(s) 12, 389
MboII GAAGA 1 cut(s) 76
MhlI GDGCHC 2 cut(s) 249, 298
MluCI AATT 2 cut(s) 26, 80
MlyI GAGTC 1 cut(s) 262
MmeI TCCRAC 2 cut(s) 271, 353
MseI TTAA 2 cut(s) 69, 156
MspA1I CMGCKG 1 cut(s) 18
MspR9I CCNGG 1 cut(s) 387
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 1 cut(s) 309
NdeII GATC 2 cut(s) 12, 389
NlaIV GGNNCC 3 cut(s) 185, 222, 384
NmeAIII GCCGAG 2 cut(s) 258, 279
PaeR7I CTCGAG 1 cut(s) 248
PkrI GCNGC 5 cut(s) 20, 53, 194, 311, 407
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
Psp124BI GAGCTC 2 cut(s) 249, 298
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 185, 222, 384
PspPI GGNCC 3 cut(s) 326, 353, 383
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 4 cut(s) 167, 185, 198, 222
RsaNI GTAC 4 cut(s) 166, 184, 197, 221
SacI GAGCTC 2 cut(s) 249, 298
SaqAI TTAA 2 cut(s) 69, 156
SatI GCNGC 5 cut(s) 19, 52, 193, 310, 406
Sau3AI GATC 2 cut(s) 12, 389
Sau96I GGNCC 3 cut(s) 326, 353, 383
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 2 cut(s) 249, 298
Sfr274I CTCGAG 1 cut(s) 248
SinI GGWCC 2 cut(s) 326, 383
SlaI CTCGAG 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 26, 80
SspMI CTAG 1 cut(s) 350
SstI GAGCTC 2 cut(s) 249, 298
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 1 cut(s) 178
TaqI TCGA 3 cut(s) 11, 96, 249
TaqII GACCGA 1 cut(s) 347
TasI AATT 2 cut(s) 26, 80
TauI GCSGC 3 cut(s) 54, 195, 408
Tru1I TTAA 2 cut(s) 69, 156
Tru9I TTAA 2 cut(s) 69, 156
TscAI CASTG 1 cut(s) 194
TseI GCWGC 2 cut(s) 18, 309
TspRI CASTG 1 cut(s) 194
VpaK11BI GGWCC 2 cut(s) 326, 383
XapI RAATTY 2 cut(s) 26, 80
XhoI CTCGAG 1 cut(s) 248
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.