Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000764

Physical Location & Seq

Genomic Coordinates Reverse (-)

30638080 .. 30643797

Gene Structure 5718 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1225500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3552 bp

ATGGGGACGCTCATTGATCGATTCGACGAGCACGATGGGCCTGTTCGCGGCGTCCACTTCCACAAATCTCAGCCCCTCTTTGTGTCTGGAGGGGATGATTATAAGATTAAAGTTTGGAACTATAAGATGCATAGGTGTCTTTTTACTCTTCTCGGGCACCTTGATTATATCCGTACGGTGCAGTTTCACCATGAGTACCCATGGATTGTGAGTGCCAGCGATGACCAGACTATTCGTATATGGAACTGGCAGTCACGTACTTGTATTTCAGTGTTGACAGGGCACAATCATTATGTCATGTGTGCCTCATTCCATCCTAAGGAGGACCTTGTTGTATCTGCCTCTCTAGATCAGACTGTCCGTGTTTGGGATATTGGTTCCCTGAAAAAGAAGACCGTATCTCCCTCAGATGACATTCTGCGACTAAGTCAGATGAACACAGATCTTTTTGGCGGTGTTGATGCTGTTGTTAAGTATGTCTTGGAAGGGCATGATCGTGGTGTCAATTGGGCTTCATTCCACCCCAACCTGCCTCTGATTGTCTCAGGAGCAGATGATCGCCAAGTTAAATTGTGGCGGATGAATGATACAAAGGCTTGGGAAGTGGATACCTTGAGAGGGCACATGAATAATGTTTCATGTGTCATGTTTCATGCCAAACAGGACATAATTGTATCTAACTCAGAGGACAAAAGTATACGTGTCTGGGATGTAACAAAGCGGACCGGCATTCAAACTTTCCGAAGAGAGCATGACCGTTTTTGGATTCTTTCATCTCACCCTGAAATGAATCTGTTGGCAGCTGGTCATGACAGTGGCATGATCGTATTTAAGCTAGAGAGGGAACGACCAGCCTTTGCAGTGAGTGGTAATTCTCTGTTCTATGCTAGAGATCGCTTTTTGCGGTACTATGAATTTTCAACCCAAAGAGACACACAAGTTATTCCCATTCGACGCCCTGGTTCCACCACTTTAAATCAAAGTCCAAGGACTGTTTCGTACAGTCCTACAGAAAATGCAGTTCTTATTTCCTCAGACTTGGATGGGGGATCTTACGAATTGTATATGATACCCAAAGACAGCATGAGTAGGGGTGATAGTCTGCAAGATGCAAAGAGAGGTGTTGGAGGTTCAGCTGTGTTTATTGCACGGAATAGATTCGCTGTGCTTGACAAAAGCAAAAACCAAGTCTTGATTAAGAATATGGCAAATGAGGCTGGTAAACAGATACCCCTTCCTTTTGCTGCTGATGCCATATTCTATGCTGGAACAAATAATTTGCTGTGTAGAGTTGAAGACAAAGTGGTTATATTTGATCTGCAGCAGAAGATTATTCTTGGTGAACTTCAAACCCCCTTCATCAAGTATGTTGTTTGGTCCAATGACATGGAAAGCGTTGCCTTGCTCAGCAAGCATGCCATTATCATTGCCAGCAAGAAGCTTGTGCACCAGTGCACTCTTCATGAGACAATTCGTGTAAAGAGTGGTGGCTGGGATGACAATGGTGTTTTCATTTACACAACTCTAAATCACATAAAATATTGCCTCCCTAATGGAGACAGTGGAATAATCAGAACCCTAGATGTTCCCATTTACATTACGAAGGTTTCTGGAAATACTATCTCTTGCTTGGATCGGGATGGGAGAAACAGGGCCATAATTATTGATGCTACGGAATACATTTTCAAGCTATCCCTATTGAAGAAGAGATATGACCATGTAATGAGCATGATAAGGAACTCACAGCTATGTGGGCAGGCAATGATTGCTTACCTGCAACAGAAGGGGTTCCCTGAAGTTGCTCTCCATTTTGTGAAAGATGAAAGAACCAGATTCAATCTGGCTCTGGAGAGTGGGAATATTCAAATTGCAGTTGCATCCGCTACAGCAATTGATGAAAAAGACTACTGGTATAGGTTGGGGGTGGAGGCGCTTCGCCAAGGCAATGCGGGTATCGTGGAGTATGCCTACCAGAAGACTAAAAATTTTGAGAGGCTATCTTTCCTTTATCTCATTACTGGTAATATGGAAAAACTGTCCAAGATGCTGAAAATTGCTGAGGTTAAGAATGATGTCATGGGTCAGTTCCACAATGCACTGTATCTAGGTAATGTCAAGGAGCGTATTAAGATCTTGGAGAATGTTGGCCACTTGCCCCTTGCTTACATCACAGCTTCAGTTCATGGCCTACATGATGTTGCTGAAAGGCTATCTGCAGAGTTGGGAGAAAATGTTCCAACTTTGCCGCAGGGGAAAGTGCCCACTCTTTTAATGCCCCCTAACCCAATTATGTGTGGCGGTGATTGGCCTCTTCTTAGAGTCATGAGAGGTATTTTTGAAGGTGGATTGGATAATGTTGGCAGGGGAGTTGTGGATGAAGAGGATGAAGCTGTTGATGGTGATTGGGGTGAGGAGCTGGACATGGTTGACGTTGCTGGCTTGCAAAATGGAGATGCCACTGCAGGATTAGAAGATGAGGAAATGGGTGAAGGACATGAGGAAGAGGAAGGATGGGACCTTGAAGATTTGGAGCTCCCCCCTGAAGCTGACACACCAAGGGCTTCTGTAAATGCTAGTTCATCGGCTTTTGTTGCTCCAACTACTGGCATGCCTGTAAACCAGATTTGGATCCAGAGATCATCTCTTGCTGCTGAACATGCAGCAGCTGGCAATTTTGACACTGCAATGCGTTTACTGGAAAGGCAACTTGGATTCAAAAACTTTGTTCCTTTAAAACCCATGTTTCTTGATCTTCACACTGGCAGCCACAGCTATCTACGTGCCTTTTCATCTGCTCCGGTAATATCTCTGGCGGTTGAGCGCGGGTGGAATGAATCTGCTACCCCGAATGTGAGAGGTCCACCAGCACTTGTGTTCAATTTTTCCCAGTTGGAAGAGAAACTGAAAGCTGGTTACAAGGCTACCACAAATGGGAAGCTCCCCGACGCTCTTAAGATCTTTCTGAGCATTCTTCACACAATTCCTTTGATTGTTGTTGATTCTAGGAGGGAAGTTGATGAAGTCAAGGAGTTAATTATAATAGTCAAAGAGTATGTTTTGGGATTACAAATTGAGCTGAAGAGAAGGGAAACGAAAGATGATCCAAAACGGGAGCAGGAGCTTGCAGCTTATTTCACACACTGCAATCTTCAAACGCCTCACTTACGGCTTGCCTTGATTAGTGCACTGACTGTTTGCTATAAGGCAGGGAACATTGCCACCGCAGCTAACTTTGCCAGGCGGCTGTTGGAAACCAACCCCACCAATGAGAGTCAAGCAAAGAAAGCCCGGCAAGTGCTGCAGGCTGCAGAAAGGAATATGACTGATAAATCTCAACTGAACTATGATTTCAGAAACCCATTTGTGATTTGTGGGGCAACACATGTACCAATTTACCGAGGACAGAGGGACGTCTCCTGCCCATATTGTAGCTCACGGTTTGTGGCAACTCAAGAAGGGCAGTTCTGTACTGTTTGTGATCTTGCAGTGGTTGGGGCAGATGCTTCTGGGTTGCTATGTTCTCCTTCTCAGTTGACCAAATCTGTCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0005886 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COPI_C Coatomer_WDAD WD40

Protein Analysis

1184

Amino Acids

132.64

Weight (kDa)

6.32

Isoelectric Point (pI)

34.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40	PF00400	3 - 40	4.8e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	5 - 88	5.3e-12	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	6 - 123	2.9e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	7 - 125	1.7e-26	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	9 - 127	1.7e-31	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	10 - 129	5.7e-09	CAF1B/HIR1 beta-propeller domain
WDR55	PF24796	10 - 129	2.5e-10	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	10 - 127	6.8e-17	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML	PF23409	10 - 125	1.9e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd	PF25172	11 - 124	9.8e-14	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	13 - 91	3e-10	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	16 - 127	1.4e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	16 - 129	1.5e-21	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	24 - 129	1.4e-08	TEP-1 second beta-propeller
Beta-prop_SCAP	PF24017	27 - 127	8.2e-06	SCAP Beta-propeller
WD40	PF00400	45 - 82	2.9e-08	WD domain, G-beta repeat
EIF3I	PF24805	49 - 126	4e-06	EIF3I
Beta-prop_EIPR1	PF23609	52 - 123	2.6e-06	EIPR1 beta-propeller
WD40_WDHD1_1st	PF24817	57 - 130	4.1e-13	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	69 - 130	5.8e-13	THOC3 beta-propeller domain
WD40	PF00400	87 - 124	1.1e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	148 - 262	2.3e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	154 - 278	2.1e-19	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	156 - 275	3.5e-18	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	157 - 251	1.1e-07	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	157 - 268	9.1e-22	WDR5 beta-propeller domain
WD40	PF00400	159 - 192	9.6e-07	WD domain, G-beta repeat
WD40_Gbeta	PF25391	159 - 249	3.9e-06	G protein beta WD-40 repeat protein
EIF3I	PF24805	160 - 248	5.3e-07	EIF3I
Beta-prop_WDR3_2nd	PF25172	162 - 277	1.5e-09	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	163 - 264	2.9e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	166 - 277	1.2e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	167 - 277	3.3e-15	THOC3 beta-propeller domain
WDR55	PF24796	168 - 276	4.1e-10	WDR55
WD40_WDHD1_1st	PF24817	172 - 248	2.8e-10	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	173 - 280	1.8e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	184 - 279	1.4e-07	CDC20/Fizzy WD40 domain
WD40	PF00400	202 - 237	1.9e-06	WD domain, G-beta repeat
B-prop_COPA_B_2nd	PF04053	301 - 545	2.3e-66	COPA/B second beta-propeller
TPR_COPA_B	PF23953	586 - 732	6.8e-19	COPA/B TPR domain
COPI_C	PF06957	776 - 1178	1.1e-165	Coatomer (COPI) alpha subunit C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62020 AT2G21390
fragaria_vesca	FvH4_3g10280 FvH4_5g12340 FvH4_5g12340 FvH4_5g12391 FvH4_5g12420
malus_domestica	MD05G1271000.v1.1 MD06G1214600.v1.1 MD10G1248600.v1.1 MD10G1248700.v1.1 MD10G1248800.v1.1 MD10G1249800.v1.1 MD10G1249900.v1.1 MD10G1250100.v1.1 MD10G1250200.v1.1 MD10G1250400.v1.1 MD10G1250600.v1.1 MD10G1250700.v1.1 MD10G1251000.v1.1 MD10G1251300.v1.1 MD10G1251600.v1.1 MD14G1225500.v1.1
prunus_persica	Prupe.4G091800_v2.0.a1 Prupe.4G091800_v2.0.a1 Prupe.5G220000_v2.0.a1 Prupe.5G220000_v2.0.a1
pyrus_communis	pycom05g25340 pycom06g19120 pycom10g20750 pycom14g18670
rosa_chinensis	RchiOBHm_Chr5g0016541 RchiOBHm_Chr7g0185121 RchiOBHm_Chr7g0185201
rosa_laevigata	RLG00000000634 RLG00000000671 RLG00000001958 RLG00000004933 RLG00000032256
rosa_multiflora	Rmu_co8505923.1_g000001 Rmu_co8507327.1_g000001 Rmu_sc0001235.1_g000018 Rmu_sc0004765.1_g000006 Rmu_sc0007192.1_g000011 Rmu_sc0019416.1_g000003 Rmu_ssc0000398.1_g000013
rosa_roxburghii	Rroxscaffold_1G00060340 Rroxscaffold_3G00269160 Rroxscaffold_3G00269190
rosa_rugosa	Rorug05G0032700 Rorug06G0466700 Rorug06G0466700 Rorug06G0466800 Rorug07G0188600
rosa_samantha	Rh4BG061900 Rh4CG068200 Rh4DG290700 Rh5AG127000 Rh5AG459900 Rh5BG125900 Rh5CG098400 Rh5CG138600 Rh5DG126100 Rh7AG076100 Rh7BG071200 Rh7CG072400 Rh7DG071500 Rh7DG071900
rosa_wichuraiana	Rw5G011000 Rw7G005980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	102, 3038
AatII	GACGTC	1 cut(s)	3414
Acc36I	ACCTGC	2 cut(s)	537, 1782
AccB1I	GGYRCC	1 cut(s)	156
AccB7I	CCANNNNNTGG	1 cut(s)	2603
AccI	GTMKAC	1 cut(s)	697
AccII	CGCG	2 cut(s)	48, 2823
AclWI	GGATC	5 cut(s)	1057, 1641, 2623, 2636, 3095
AcoI	YGGCCR	1 cut(s)	2146
AcsI	RAATTY	2 cut(s)	914, 1984
AcuI	CTGAAG	4 cut(s)	1815, 2160, 2562, 3098
AcyI	GRCGYC	3 cut(s)	51, 955, 3411
AfaI	GTAC	7 cut(s)	175, 197, 259, 908, 1001, 3387, 3469
AfiI	CCNNNNNNNGG	6 cut(s)	47, 319, 367, 618, 2603, 2931
AflII	CTTAAG	1 cut(s)	2951
AflIII	ACRYGT	2 cut(s)	700, 3382
AjnI	CCWGG	2 cut(s)	958, 3236
AjuI	GAANNNNNNNTTGG	4 cut(s)	979, 1011, 2691, 2723
AloI	GAACNNNNNNTCC	2 cut(s)	2217, 2249
Alw21I	GWGCWC	5 cut(s)	33, 1449, 1457, 2535, 3187
Alw26I	GTCTC	5 cut(s)	547, 924, 1460, 1551, 3418
Alw44I	GTGCAC	3 cut(s)	1445, 1453, 3183
AlwI	GGATC	5 cut(s)	1057, 1641, 2623, 2636, 3095
AlwNI	CAGNNNCTG	1 cut(s)	2666
Ama87I	CYCGRG	1 cut(s)	152
AoxI	GGCC	5 cut(s)	38, 1653, 2146, 2185, 2306
ApaLI	GTGCAC	3 cut(s)	1445, 1453, 3183
ApoI	RAATTY	2 cut(s)	914, 1984
ArsI	GACNNNNNNTTYG	2 cut(s)	1173, 1205
Asp700I	GAANNNNTTC	3 cut(s)	1157, 1787, 2232
AspLEI	GCGC	2 cut(s)	1933, 2823
AspS9I	GGNCC	7 cut(s)	38, 325, 723, 1377, 1653, 2515, 2858
AsuC2I	CCSGG	1 cut(s)	3289
AsuHPI	GGTGA	8 cut(s)	179, 770, 1106, 1352, 2312, 2411, 2420, 2498
AvaI	CYCGRG	1 cut(s)	152
AvaII	GGWCC	5 cut(s)	325, 723, 1377, 2515, 2858
AxyI	CCTNAGG	1 cut(s)	318
BaeGI	GKGCMC	7 cut(s)	159, 285, 624, 1449, 1457, 2262, 3187
BaeI	ACNNNNGTAYC	2 cut(s)	3369, 3402
BalI	TGGCCA	1 cut(s)	2148
BamHI	GGATCC	1 cut(s)	2628
BanI	GGYRCC	1 cut(s)	156
BanII	GRGCYC	1 cut(s)	2535
BbsI	GAAGAC	3 cut(s)	398, 1302, 1982
Bbv12I	GWGCWC	5 cut(s)	33, 1449, 1457, 2535, 3187
BbvCI	CCTCAGC	1 cut(s)	2058
BccI	CCATC	6 cut(s)	29, 321, 1037, 1634, 2390, 2505
BceAI	ACGGC	1 cut(s)	3182
BciT130I	CCWGG	2 cut(s)	960, 3238
BciVI	GTATCC	1 cut(s)	601
BcnI	CCSGG	1 cut(s)	3289
BcoDI	GTCTC	5 cut(s)	547, 924, 1460, 1551, 3418
BfaI	CTAG	7 cut(s)	347, 836, 888, 1580, 2105, 2574, 3003
BfmI	CTRYAG	7 cut(s)	1008, 1319, 1884, 2214, 2460, 3299, 3306
BfoI	RGCGCY	1 cut(s)	1934
BfrI	CTTAAG	1 cut(s)	2951
BfuAI	ACCTGC	2 cut(s)	537, 1782
BfuI	GTATCC	1 cut(s)	601
BglII	AGATCT	3 cut(s)	442, 2130, 2955
BlpI	GCTNAGC	1 cut(s)	1406
Bme1390I	CCNGG	3 cut(s)	960, 3238, 3289
Bme18I	GGWCC	5 cut(s)	325, 723, 1377, 2515, 2858
BmeT110I	CYCGRG	1 cut(s)	152
BmgT120I	GGNCC	7 cut(s)	38, 325, 723, 1377, 1653, 2515, 2858
BmiI	GGNNCC	6 cut(s)	158, 379, 964, 1790, 2516, 2630
BmrFI	CCNGG	3 cut(s)	960, 3238, 3289
BmrI	ACTGGG	1 cut(s)	2881
BmsI	GCATC	9 cut(s)	117, 451, 1099, 1240, 1657, 1886, 2034, 2443, 3490
BmuI	ACTGGG	1 cut(s)	2881
BpiI	GAAGAC	3 cut(s)	398, 1302, 1982
BplI	GAGNNNNNCTC	2 cut(s)	2626, 2658
BpmI	CTGGAG	2 cut(s)	108, 1868
Bpu10I	CCTNAGC	1 cut(s)	2058
Bpu1102I	GCTNAGC	1 cut(s)	1406
BpuEI	CTTGAG	2 cut(s)	634, 3435
BpuMI	CCSGG	1 cut(s)	3289
Bsa29I	ATCGAT	1 cut(s)	19
BsaAI	YACGTR	3 cut(s)	257, 701, 2780
BsaBI	GATNNNNATC	1 cut(s)	2627
BsaHI	GRCGYC	3 cut(s)	51, 955, 3411
BsaJI	CCNNGG	6 cut(s)	200, 958, 986, 1939, 2555, 3397
BsaWI	WCCGGW	1 cut(s)	2797
BsaXI	ACNNNNNCTCC	4 cut(s)	385, 415, 2217, 2247
Bsc4I	CCNNNNNNNGG	6 cut(s)	47, 319, 367, 618, 2603, 2931
Bse118I	RCCGGY	1 cut(s)	725
Bse1I	ACTGG	8 cut(s)	251, 1450, 1913, 2023, 2608, 2700, 2764, 2887
Bse21I	CCTNAGG	1 cut(s)	318
Bse3DI	GCAATG	5 cut(s)	1425, 1767, 1951, 2691, 3213
Bse8I	GATNNNNATC	1 cut(s)	2627
BseBI	CCWGG	2 cut(s)	960, 3238
BseCI	ATCGAT	1 cut(s)	19
BseDI	CCNNGG	6 cut(s)	200, 958, 986, 1939, 2555, 3397
BseJI	GATNNNNATC	1 cut(s)	2627
BseLI	CCNNNNNNNGG	6 cut(s)	47, 319, 367, 618, 2603, 2931
BseMI	GCAATG	5 cut(s)	1425, 1767, 1951, 2691, 3213
BseMII	CTCAG	9 cut(s)	83, 420, 558, 696, 1047, 1420, 2049, 2954, 3542
BseNI	ACTGG	8 cut(s)	251, 1450, 1913, 2023, 2608, 2700, 2764, 2887
BseRI	GAGGAG	1 cut(s)	2426
BseSI	GKGCMC	7 cut(s)	159, 285, 624, 1449, 1457, 2262, 3187
BseYI	CCCAGC	1 cut(s)	1491
BsgI	GTGCAG	1 cut(s)	200
Bsh1236I	CGCG	2 cut(s)	48, 2823
BshFI	GGCC	5 cut(s)	40, 1655, 2148, 2187, 2308
BshNI	GGYRCC	1 cut(s)	156
BshVI	ATCGAT	1 cut(s)	19
BsiHKAI	GWGCWC	5 cut(s)	33, 1449, 1457, 2535, 3187
BsiHKCI	CYCGRG	1 cut(s)	152
BsiSI	CCGG	3 cut(s)	726, 2798, 3289
BsiWI	CGTACG	1 cut(s)	173
BslFI	GGGAC	3 cut(s)	19, 2528, 3422
BslI	CCNNNNNNNGG	6 cut(s)	47, 319, 367, 618, 2603, 2931
BsmAI	GTCTC	5 cut(s)	547, 924, 1460, 1551, 3418
BsmBI	CGTCTC	1 cut(s)	3418
BsmFI	GGGAC	3 cut(s)	19, 2528, 3422
BsmI	GAATGC	2 cut(s)	729, 2967
BsnI	GGCC	5 cut(s)	40, 1655, 2148, 2187, 2308
BsoBI	CYCGRG	1 cut(s)	152
Bsp1286I	GDGCHC	9 cut(s)	33, 159, 285, 624, 1449, 1457, 2262, 2535, 3187
Bsp1720I	GCTNAGC	1 cut(s)	1406
Bsp19I	CCATGG	1 cut(s)	200
BspANI	GGCC	5 cut(s)	40, 1655, 2148, 2187, 2308
BspCNI	CTCAG	9 cut(s)	82, 419, 557, 695, 1046, 1419, 2050, 2955, 3541
BspDI	ATCGAT	1 cut(s)	19
BspFNI	CGCG	2 cut(s)	48, 2823
BspHI	TCATGA	3 cut(s)	808, 1462, 2322
BspLI	GGNNCC	6 cut(s)	158, 379, 964, 1790, 2516, 2630
BspMAI	CTGCAG	5 cut(s)	1323, 2218, 2464, 3303, 3310
BspMI	ACCTGC	2 cut(s)	537, 1782
BspPI	GGATC	5 cut(s)	1057, 1641, 2623, 2636, 3095
BspT107I	GGYRCC	1 cut(s)	156
BspTI	CTTAAG	1 cut(s)	2951
BsrDI	GCAATG	5 cut(s)	1425, 1767, 1951, 2691, 3213
BsrFI	RCCGGY	1 cut(s)	725
BsrI	ACTGG	8 cut(s)	251, 1450, 1913, 2023, 2608, 2700, 2764, 2887
BssAI	RCCGGY	1 cut(s)	725
BssECI	CCNNGG	6 cut(s)	200, 958, 986, 1939, 2555, 3397
BssNAI	GTATAC	1 cut(s)	698
BssNI	GRCGYC	3 cut(s)	51, 955, 3411
BssT1I	CCWWGG	4 cut(s)	200, 986, 1939, 2555
Bst1107I	GTATAC	1 cut(s)	698
Bst2UI	CCWGG	2 cut(s)	960, 3238
Bst6I	CTCTTC	9 cut(s)	153, 739, 1464, 1700, 2316, 2373, 2496, 2889, 3074
BstACI	GRCGYC	3 cut(s)	51, 955, 3411
BstAFI	CTTAAG	1 cut(s)	2951
BstAPI	GCANNNNNTGC	2 cut(s)	1766, 3298
BstBAI	YACGTR	3 cut(s)	257, 701, 2780
BstDSI	CCRYGG	1 cut(s)	200
BstFNI	CGCG	2 cut(s)	48, 2823
BstH2I	RGCGCY	1 cut(s)	1934
BstHHI	GCGC	2 cut(s)	1933, 2823
BstMAI	GTCTC	5 cut(s)	547, 924, 1460, 1551, 3418
BstNI	CCWGG	2 cut(s)	960, 3238
BstNSI	RCATGY	4 cut(s)	1418, 2611, 2660, 3386
BstSCI	CCNGG	3 cut(s)	958, 3236, 3287
BstSFI	CTRYAG	7 cut(s)	1008, 1319, 1884, 2214, 2460, 3299, 3306
BstSLI	GKGCMC	7 cut(s)	159, 285, 624, 1449, 1457, 2262, 3187
BstUI	CGCG	2 cut(s)	48, 2823
BstV2I	GAAGAC	3 cut(s)	398, 1302, 1982
BstX2I	RGATCY	5 cut(s)	442, 1049, 2130, 2628, 2955
BstXI	CCANNNNNNTGG	1 cut(s)	1387
BstYI	RGATCY	5 cut(s)	442, 1049, 2130, 2628, 2955
BstZ17I	GTATAC	1 cut(s)	698
Bsu15I	ATCGAT	1 cut(s)	19
Bsu36I	CCTNAGG	1 cut(s)	318
BsuI	GTATCC	1 cut(s)	601
BsuRI	GGCC	5 cut(s)	40, 1655, 2148, 2187, 2308
BsuTUI	ATCGAT	1 cut(s)	19
BtgI	CCRYGG	1 cut(s)	200
BtgZI	GCGATG	1 cut(s)	234
BtsI	GCAGTG	5 cut(s)	867, 2457, 2679, 3139, 3492
BveI	ACCTGC	2 cut(s)	537, 1782
CaiI	CAGNNNCTG	1 cut(s)	2666
CciI	TCATGA	3 cut(s)	808, 1462, 2322
CfoI	GCGC	2 cut(s)	1933, 2823
Cfr10I	RCCGGY	1 cut(s)	725
Cfr13I	GGNCC	7 cut(s)	38, 325, 723, 1377, 1653, 2515, 2858
ClaI	ATCGAT	1 cut(s)	19
CpoI	CGGWCCG	1 cut(s)	723
CseI	GACGC	4 cut(s)	16, 40, 963, 2954
Csp6I	GTAC	7 cut(s)	174, 196, 258, 907, 1000, 3386, 3468
CspI	CGGWCCG	1 cut(s)	723
CviQI	GTAC	7 cut(s)	174, 196, 258, 907, 1000, 3386, 3468
DraI	TTTAAA	2 cut(s)	975, 2733
EaeI	YGGCCR	1 cut(s)	2146
Eam1104I	CTCTTC	9 cut(s)	153, 739, 1464, 1700, 2316, 2373, 2496, 2889, 3074
EarI	CTCTTC	9 cut(s)	153, 739, 1464, 1700, 2316, 2373, 2496, 2889, 3074
EciI	GGCGGA	1 cut(s)	592
Ecl136II	GAGCTC	1 cut(s)	2533
Eco130I	CCWWGG	4 cut(s)	200, 986, 1939, 2555
Eco24I	GRGCYC	1 cut(s)	2535
Eco47I	GGWCC	5 cut(s)	325, 723, 1377, 2515, 2858
Eco53kI	GAGCTC	1 cut(s)	2533
Eco57I	CTGAAG	4 cut(s)	1815, 2160, 2562, 3098
Eco81I	CCTNAGG	1 cut(s)	318
Eco88I	CYCGRG	1 cut(s)	152
EcoICRI	GAGCTC	1 cut(s)	2533
EcoO109I	RGGNCCY	2 cut(s)	325, 2515
EcoRII	CCWGG	2 cut(s)	958, 3236
EcoT14I	CCWWGG	4 cut(s)	200, 986, 1939, 2555
EcoT22I	ATGCAT	1 cut(s)	132
EcoT38I	GRGCYC	1 cut(s)	2535
ErhI	CCWWGG	4 cut(s)	200, 986, 1939, 2555
Esp3I	CGTCTC	1 cut(s)	3418
FalI	AAGNNNNNCTT	2 cut(s)	464, 496
FaqI	GGGAC	3 cut(s)	19, 2528, 3422
FauI	CCCGC	2 cut(s)	1942, 2816
FblI	GTMKAC	1 cut(s)	697
FriOI	GRGCYC	1 cut(s)	2535
FspBI	CTAG	7 cut(s)	347, 836, 888, 1580, 2105, 2574, 3003
GlaI	GCGC	2 cut(s)	1932, 2822
GsaI	CCCAGC	1 cut(s)	1495
GsuI	CTGGAG	2 cut(s)	108, 1868
HaeII	RGCGCY	1 cut(s)	1934
HaeIII	GGCC	5 cut(s)	40, 1655, 2148, 2187, 2308
HapII	CCGG	3 cut(s)	726, 2798, 3289
HgaI	GACGC	4 cut(s)	16, 40, 963, 2954
HhaI	GCGC	2 cut(s)	1933, 2823
Hin1I	GRCGYC	3 cut(s)	51, 955, 3411
Hin6I	GCGC	2 cut(s)	1931, 2821
HinP1I	GCGC	2 cut(s)	1931, 2821
HincII	GTYRAC	3 cut(s)	276, 2428, 3534
HindII	GTYRAC	3 cut(s)	276, 2428, 3534
HindIII	AAGCTT	1 cut(s)	1439
HpaII	CCGG	3 cut(s)	726, 2798, 3289
HphI	GGTGA	8 cut(s)	179, 770, 1106, 1352, 2312, 2411, 2420, 2498
Hpy99I	CGWCG	3 cut(s)	29, 957, 2948
HpyCH4IV	ACGT	5 cut(s)	256, 700, 2430, 2779, 3411
HpySE526I	ACGT	5 cut(s)	256, 700, 2430, 2779, 3411
Hsp92I	GRCGYC	3 cut(s)	51, 955, 3411
HspAI	GCGC	2 cut(s)	1931, 2821
LweI	GCATC	9 cut(s)	117, 451, 1099, 1240, 1657, 1886, 2034, 2443, 3490
MaeI	CTAG	7 cut(s)	347, 836, 888, 1580, 2105, 2574, 3003
MaeII	ACGT	5 cut(s)	256, 700, 2430, 2779, 3411
MaeIII	GTNAC	3 cut(s)	252, 712, 2912
MfeI	CAATTG	2 cut(s)	505, 1890
MflI	RGATCY	5 cut(s)	442, 1049, 2130, 2628, 2955
MhlI	GDGCHC	9 cut(s)	33, 159, 285, 624, 1449, 1457, 2262, 2535, 3187
MlsI	TGGCCA	1 cut(s)	2148
MluNI	TGGCCA	1 cut(s)	2148
MlyI	GAGTC	2 cut(s)	2328, 3280
MmeI	TCCRAC	5 cut(s)	1105, 2261, 2621, 2871, 3228
Mox20I	TGGCCA	1 cut(s)	2148
Mph1103I	ATGCAT	1 cut(s)	132
MroXI	GAANNNNTTC	3 cut(s)	1157, 1787, 2232
MscI	TGGCCA	1 cut(s)	2148
MslI	CAYNNNNRTG	4 cut(s)	495, 813, 1747, 2684
Msp20I	TGGCCA	1 cut(s)	2148
MspA1I	CMGCKG	3 cut(s)	803, 1136, 2666
MspCI	CTTAAG	1 cut(s)	2951
MspI	CCGG	3 cut(s)	726, 2798, 3289
MspR9I	CCNGG	3 cut(s)	960, 3238, 3289
MunI	CAATTG	2 cut(s)	505, 1890
Mva1269I	GAATGC	2 cut(s)	729, 2967
MvaI	CCWGG	2 cut(s)	960, 3238
MvnI	CGCG	2 cut(s)	48, 2823
NciI	CCSGG	1 cut(s)	3289
NcoI	CCATGG	1 cut(s)	200
NlaIV	GGNNCC	6 cut(s)	158, 379, 964, 1790, 2516, 2630
NmuCI	GTSAC	1 cut(s)	252
NsiI	ATGCAT	1 cut(s)	132
NspI	RCATGY	4 cut(s)	1418, 2611, 2660, 3386
PaeI	GCATGC	2 cut(s)	1418, 2611
PagI	TCATGA	3 cut(s)	808, 1462, 2322
PciI	ACATGT	1 cut(s)	3382
PcsI	WCGNNNNNNNCGW	1 cut(s)	30
PctI	GAATGC	2 cut(s)	729, 2967
PdmI	GAANNNNTTC	3 cut(s)	1157, 1787, 2232
PfeI	GAWTC	8 cut(s)	21, 766, 790, 1158, 1833, 2712, 2834, 2999
Pfl23II	CGTACG	1 cut(s)	173
PflFI	GACNNNGTC	1 cut(s)	426
PflMI	CCANNNNNTGG	1 cut(s)	2603
PleI	GAGTC	2 cut(s)	2327, 3279
PpsI	GAGTC	2 cut(s)	2327, 3279
Ppu21I	YACGTR	3 cut(s)	257, 701, 2780
PpuMI	RGGWCCY	2 cut(s)	325, 2515
PscI	ACATGT	1 cut(s)	3382
PsiI	TTATAA	2 cut(s)	102, 3038
Psp124BI	GAGCTC	1 cut(s)	2535
Psp5II	RGGWCCY	2 cut(s)	325, 2515
Psp6I	CCWGG	2 cut(s)	958, 3236
PspFI	CCCAGC	1 cut(s)	1491
PspGI	CCWGG	2 cut(s)	958, 3236
PspLI	CGTACG	1 cut(s)	173
PspN4I	GGNNCC	6 cut(s)	158, 379, 964, 1790, 2516, 2630
PspPI	GGNCC	7 cut(s)	38, 325, 723, 1377, 1653, 2515, 2858
PspPPI	RGGWCCY	2 cut(s)	325, 2515
PstI	CTGCAG	5 cut(s)	1323, 2218, 2464, 3303, 3310
PstNI	CAGNNNCTG	1 cut(s)	2666
PsuI	RGATCY	5 cut(s)	442, 1049, 2130, 2628, 2955
PsyI	GACNNNGTC	1 cut(s)	426
PvuII	CAGCTG	3 cut(s)	803, 1136, 2666
RsaI	GTAC	7 cut(s)	175, 197, 259, 908, 1001, 3387, 3469
RsaNI	GTAC	7 cut(s)	174, 196, 258, 907, 1000, 3386, 3468
RseI	CAYNNNNRTG	4 cut(s)	495, 813, 1747, 2684
Rsr2I	CGGWCCG	1 cut(s)	723
RsrII	CGGWCCG	1 cut(s)	723
SacI	GAGCTC	1 cut(s)	2535
Sau96I	GGNCC	7 cut(s)	38, 325, 723, 1377, 1653, 2515, 2858
SchI	GAGTC	2 cut(s)	2328, 3280
ScrFI	CCNGG	3 cut(s)	960, 3238, 3289
SduI	GDGCHC	9 cut(s)	33, 159, 285, 624, 1449, 1457, 2262, 2535, 3187
SfaNI	GCATC	9 cut(s)	117, 451, 1099, 1240, 1657, 1886, 2034, 2443, 3490
SfcI	CTRYAG	7 cut(s)	1008, 1319, 1884, 2214, 2460, 3299, 3306
SinI	GGWCC	5 cut(s)	325, 723, 1377, 2515, 2858
SmiMI	CAYNNNNRTG	4 cut(s)	495, 813, 1747, 2684
SmlI	CTYRAG	3 cut(s)	613, 2951, 3450
SmoI	CTYRAG	3 cut(s)	613, 2951, 3450
SphI	GCATGC	2 cut(s)	1418, 2611
SspI	AATATT	2 cut(s)	1541, 1861
SspMI	CTAG	7 cut(s)	347, 836, 888, 1580, 2105, 2574, 3003
SstI	GAGCTC	1 cut(s)	2535
StyD4I	CCNGG	3 cut(s)	958, 3236, 3287
StyI	CCWWGG	4 cut(s)	200, 986, 1939, 2555
TaiI	ACGT	5 cut(s)	259, 703, 2433, 2782, 3414
TaqI	TCGA	3 cut(s)	19, 24, 952
TatI	WGTACW	1 cut(s)	3467
TauI	GCSGC	3 cut(s)	51, 2248, 3244
TfiI	GAWTC	8 cut(s)	21, 766, 790, 1158, 1833, 2712, 2834, 2999
TseFI	GTSAC	1 cut(s)	252
Tsp45I	GTSAC	1 cut(s)	252
TspGWI	ACGGA	4 cut(s)	161, 350, 1165, 1688
Tth111I	GACNNNGTC	1 cut(s)	426
Van91I	CCANNNNNTGG	1 cut(s)	2603
Vha464I	CTTAAG	1 cut(s)	2951
VneI	GTGCAC	3 cut(s)	1445, 1453, 3183
VpaK11BI	GGWCC	5 cut(s)	325, 723, 1377, 2515, 2858
XapI	RAATTY	2 cut(s)	914, 1984
XbaI	TCTAGA	1 cut(s)	346
XceI	RCATGY	4 cut(s)	1418, 2611, 2660, 3386
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1837, 3244
XmiI	GTMKAC	1 cut(s)	697
XmnI	GAANNNNTTC	3 cut(s)	1157, 1787, 2232
XspI	CTAG	7 cut(s)	347, 836, 888, 1580, 2105, 2574, 3003
ZraI	GACGTC	1 cut(s)	3412
Zsp2I	ATGCAT	1 cut(s)	132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.