Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0000764

Physical Location & Seq

Genomic Coordinates Reverse (-)

4832694 .. 4839861

Gene Structure 7168 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG071200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3417 bp

ATGCATAGGTGTCTTTTTACTCTTCTCGGGCATCTAGATTATATCCGAACCGTGCAATTTCACCATGAGTACCCATGGATTGTGAGTGCCAGTGATGACCAGACTATTCGTATATGGAACTGGCAGTCGCGCACTTGTATTTCAGTTTTGACTGGCCACAATCATTATGTTATGTGTGCCTCATTCCATCCTAAAGAAGACCTTGTCGTATCTGCCTCCTTGGATCAGACTGTCCGTGTTTGGGATATTGGTTCTCTGAAAAAGAAGTCTGTGTCCCCTGCGGATGATATACTGCGACTAAGTCAGATGAACACAGATCTTTTTGGTGGTGTTGATGCTGTTGTTAAGTATGTCTTGGAAGGGCATGACCGAGGTGTCAATTGGGCTTCATTCCACCCCAACCTGCCCCTCATTGTCTCAGGAGCAGACGATCGCCAAGTTAAATTGTGGCGGATGAATGATACTAAGGCTTGGGAAGTGGACACGTTGAGAGGTCACATGAATAATGTTTCATGTGTTATGTTTCATGCTAAGCAGGATATAATCGTATCCAACTCAGAGGACAAAAGTATCCGTGTCTGGGATGTGACAAAGCGAACTGGAATTCAAACTTTCCGACGAGAGCATGATCGCTTTTGGATTCTTTCATCTCACCCTGAAATGAATCTATTAGCAGCAGGTCATGACAGTGGGATGATCGTCTTTAAGTTAGAGAGGGAAAGACCGGCCTTTGCCGTCAGTGGTGATTCTCTGTTCTATGCTAAAGACCGCTTTTTGAAGTTCTATGAGTTTTCAACCCAGAGAGATACACAAGTTATTCCAATTCGACGACCTGGTTCTGTCACTTTAAATCAAAGTCCAAGGACCCTTTCTTACAGTCCTTCAGAAAATGCAGTTCTTGTTTGCTCAGACTTGGACGGGGGATCCTATGAGTTGTATTTCATACCCAGAGACAGCATTACTAGGGGTGACAGTGCGCAAGATGCTAAGAGAGGTGTTGGTGGTTCAGCTGTGTTTGTAGCACGGAATAGGTTTGCTGTGCTTGAGAAAAGCACAAACTCTGTCCTGGTAAAGAATTTGAGGAATGAGGTTGTTAAAAAGACTCCCCTTCCTTTTGCTGCTGATGCAATATTCTACGCTGGAACAGGTAATTTGCTGTGTAGGTCAGAGGACAAAGTGTTTATATTTGATCTCCAGCAGAGGACTGTTCTTGGTGAACTTCAAACCCCCTTCGTCAAGTATGTTGTTTGGTCTAATGACATGGAAAGTATTGCCTTGCTCAGCAAACATGCCATAATTATTGCTAGCAAGAGGCTTGTGCACCAGTGCACTCTTCATGAGACAATCCGTGTAAAGAGTGGTGGTTGGGATGACAACGGTGTTTTCATTTATTCAACTCTGAATCACATTAAATATTGTCTACCTAATGGAGATAGTGGAATAATCAGAACGCTTGATGTTCCTATCTACATTACGAAAGTTTCTGCAAATACTATCTTTTGCTTGGATCGGGATGGGAAAAATAAGGCCATAGTTATTGATGCCACAGAATACATCTTCAAGTTATCCCTTTTGAAGAAGAAATATGACAATGTTATGAGCATGATACGGAACTCACAGCTCTGTGGGCAGGCGATGATAGCTTATCTGCAACAGAAGGGGTTCCCCGAAGTTGCCCTCCATTTTGTGAAAGATGAAAGAACTCGATTCAATCTGGCATTAGAGAGTGGGAATATTCAAATTGCGGTTGAATCTGCCACAGCAATTGATGAAAAAGATTACTGGTATAGGTTGGGTGTGGAGGCTCTTCGACAGGGCAATGCAGGTATTGTAGAGTTTGCCTACCAGAGGACCAAAAATTTTGAAAGGCTGTCTTTCCTTTATCTCATAAATGGAAATACGCAGAAACTGTCCAAGATGCTGAAAATTGCTGAGGTCAAGAATGATGTCATGGGTCAGTTTCACAATGCACTGTATCTGGGGAATGTTGAGGAGCGCATTAAGATATTGGAGAATGTTGGTCACTTGCCCCTTGCTTATATTACAGCAAAAACTCATGGGCTGCATGATGTTGCCGAAAGGCTAGCAGCTGAGTTGGGGGATAATCTTCCAACTTTGCCCGAGGGTAAAGCACCACCAACTCTTTTGACTCCCCCAACACCAATCATGTGTGGTAGTGATTGGCCACTTCTCAGAGTAATGAGAGGTATATTTGAAGGTGGGTTGGATAATATCGGCAGGGGAGCTGTGGATGAAGAGGATGAGGCTGTTGATGGTGACTGGGGTGAGGAGCTGGACATGGTTGATGTTGATGGGTTACAAAATGGAGTTGTCCTTCCAGGTTTGGAAGGTGAGGAAGTGCCTGGAGAAGATGATGGAGAGGGAGGATGGGACCTTGAAGATTTGGAGCTCCCCCCTGAAGCTGACACACCTAGGGCTTCTGTTAATACTCATTCATCAGTTTTTGTTGCTCCAACTACTGGCATGCCTGTAAGCCAGATTTGGATCCAGAGATCATCTCTTGCCGCTGAGCATGCCGCTGCAGGCCATTTTGATACTGCGATGCGGTTGCTGAACAGGCAACTTGGGATTAAAAACTTCACTCCTTTGAGGCAATTGTTCCTCGATCTTCACTCTGGAAGCCACAGCTATCTACGGGCATTTTCGTCTGCTCCGGTGATATCATTAGCAGTTGAGCGTGGGTGGAATGAAACTACCACGCCAAATGTTAGAAACCCACCAGCACTTGTGTTCAATTTTTCTCAGTTGGAAGAGAAACTCAAAGCTGGTTACAAGGCTACAACTGCTGGGAAGTTCACTGAAGCTCTCAGGCTCTTTCTTAGCATTCTTCATACAATTCCTTTGATTGTTGTTGATTCAAGGAGAGAAGTTGATGAAGTTAAGGAGTTAATTGTAATAGTCAAGGAATATGTTCTGGGATTGCAAATGGAGCTGAAGAGAAGGGAAATGAAAGATAATCCAGTACGTCAGCAGGAGCTTGCAGCTTATTTCACACACTGCAATCTTCAATTGCCTCACTTAAGGCTTGCTTTGCTTAATGCAATGACAGTTTGCTACAAGGCAAAGAACCTTGCCACTGCAGCTAACTTTGCCAGACGGCTATTGGAGACGAACCCCACCGTTGAGAGTCAGGCAAAGTTACCCAGGCAAGTGCTGCAGGCGGCAGAGAAGAATATGACTGATGCTTCTCAACTGAACTATGACTTCAGAAATCCATTTGTGACTTGTGGGGCAACATATGTACCAATTTACCGAGGACAGAAGGACGTCTCTTGCCCTTACTGTAGCTCACGGTTTGTGCCAAGCCAAGAAGGGAAGCTCTGTACTGTTTGTGATCTTGCAGTGGTTGGTGCAGATGCTTCGGGGTTGCTATGTTCTCCATCTCAGATTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0005886 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COPI_C Coatomer_WDAD WD40

Protein Analysis

1138

Amino Acids

127.52

Weight (kDa)

6.36

Isoelectric Point (pI)

34.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	3 - 83	5.2e-17	WDR3 first beta-propeller domain
WD40	PF00400	3 - 40	2.8e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	4 - 85	4.6e-24	WDR5 beta-propeller domain
WD40_Prp19	PF24814	5 - 87	4e-15	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	7 - 82	5.4e-09	WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	7 - 87	4.7e-08	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	7 - 85	3.7e-06	EIF3I
Beta-prop_EIPR1	PF23609	10 - 81	2.6e-06	EIPR1 beta-propeller
WD40_CDC20-Fz	PF24807	11 - 204	1.7e-10	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	12 - 88	2.5e-14	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	15 - 85	2e-07	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	15 - 88	3.8e-13	WDHD1 first WD40 domain
WD40	PF00400	45 - 82	1e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	101 - 222	1.7e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	112 - 236	1.9e-19	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	113 - 233	3.3e-18	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	114 - 226	7.8e-22	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	115 - 209	1.1e-07	TEP-1 second beta-propeller
WD40	PF00400	117 - 150	9.2e-07	WD domain, G-beta repeat
WD40_Gbeta	PF25391	117 - 207	4.5e-06	G protein beta WD-40 repeat protein
EIF3I	PF24805	118 - 206	5e-07	EIF3I
Beta-prop_WDR3_2nd	PF25172	120 - 235	1.4e-09	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	121 - 222	2.9e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	124 - 235	1.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	125 - 235	3.1e-15	THOC3 beta-propeller domain
WDR55	PF24796	126 - 234	3.9e-10	WDR55
WD40_WDHD1_1st	PF24817	130 - 205	4e-10	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	131 - 237	2.2e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	142 - 237	1.4e-07	CDC20/Fizzy WD40 domain
WD40	PF00400	160 - 195	1.8e-06	WD domain, G-beta repeat
B-prop_COPA_B_2nd	PF04053	259 - 503	3.7e-68	COPA/B second beta-propeller
TPR_COPA_B	PF23953	532 - 692	5.5e-20	COPA/B TPR domain
COPI_C	PF06957	735 - 1138	4.7e-169	Coatomer (COPI) alpha subunit C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62020 AT2G21390
fragaria_vesca	FvH4_3g10280 FvH4_5g12340 FvH4_5g12340 FvH4_5g12391 FvH4_5g12420
malus_domestica	MD05G1271000.v1.1 MD06G1214600.v1.1 MD10G1248600.v1.1 MD10G1248700.v1.1 MD10G1248800.v1.1 MD10G1249800.v1.1 MD10G1249900.v1.1 MD10G1250100.v1.1 MD10G1250200.v1.1 MD10G1250400.v1.1 MD10G1250600.v1.1 MD10G1250700.v1.1 MD10G1251000.v1.1 MD10G1251300.v1.1 MD10G1251600.v1.1 MD14G1225500.v1.1
prunus_persica	Prupe.4G091800_v2.0.a1 Prupe.4G091800_v2.0.a1 Prupe.5G220000_v2.0.a1 Prupe.5G220000_v2.0.a1
pyrus_communis	pycom05g25340 pycom06g19120 pycom10g20750 pycom14g18670
rosa_chinensis	RchiOBHm_Chr5g0016541 RchiOBHm_Chr7g0185121 RchiOBHm_Chr7g0185201
rosa_laevigata	RLG00000000634 RLG00000000671 RLG00000001958 RLG00000004933 RLG00000032256
rosa_multiflora	Rmu_co8505923.1_g000001 Rmu_co8507327.1_g000001 Rmu_sc0001235.1_g000018 Rmu_sc0004765.1_g000006 Rmu_sc0007192.1_g000011 Rmu_sc0019416.1_g000003 Rmu_ssc0000398.1_g000013
rosa_roxburghii	Rroxscaffold_1G00060340 Rroxscaffold_3G00269160 Rroxscaffold_3G00269190
rosa_rugosa	Rorug05G0032700 Rorug06G0466700 Rorug06G0466700 Rorug06G0466800 Rorug07G0188600
rosa_samantha	Rh4BG061900 Rh4CG068200 Rh4DG290700 Rh5AG127000 Rh5AG459900 Rh5BG125900 Rh5CG098400 Rh5CG138600 Rh5DG126100 Rh7AG076100 Rh7BG071200 Rh7CG072400 Rh7DG071500 Rh7DG071900
rosa_wichuraiana	Rw5G011000 Rw7G005980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	374
AatII	GACGTC	1 cut(s)	3291
Acc16I	TGCGCA	1 cut(s)	978
Acc36I	ACCTGC	3 cut(s)	411, 668, 1814
AccB7I	CCANNNNNTGG	1 cut(s)	2480
AccI	GTMKAC	1 cut(s)	1420
AccII	CGCG	1 cut(s)	130
AciI	CCGC	8 cut(s)	281, 451, 769, 1745, 2526, 2538, 2566, 3182
AclWI	GGATC	6 cut(s)	231, 918, 931, 1515, 2500, 2513
AcoI	YGGCCR	2 cut(s)	154, 2183
AcsI	RAATTY	3 cut(s)	603, 1075, 1858
AcuI	CTGAAG	5 cut(s)	867, 2439, 2841, 2975, 3211
AcyI	GRCGYC	1 cut(s)	3288
AfaI	GTAC	4 cut(s)	71, 2985, 3264, 3346
AfiI	CCNNNNNNNGG	4 cut(s)	241, 580, 1848, 2480
AflII	CTTAAG	1 cut(s)	3040
AflIII	ACRYGT	1 cut(s)	483
AjnI	CCWGG	5 cut(s)	832, 1065, 2338, 2362, 3164
AjuI	GAANNNNNNNTTGG	2 cut(s)	853, 885
Alw21I	GWGCWC	3 cut(s)	1323, 1331, 2412
Alw26I	GTCTC	5 cut(s)	421, 945, 1334, 3122, 3295
Alw44I	GTGCAC	2 cut(s)	1319, 1327
AlwI	GGATC	6 cut(s)	231, 918, 931, 1515, 2500, 2513
AlwNI	CAGNNNCTG	1 cut(s)	1909
Ama87I	CYCGRG	2 cut(s)	26, 2120
AoxI	GGCC	5 cut(s)	154, 726, 1527, 2183, 2545
ApaLI	GTGCAC	2 cut(s)	1319, 1327
ApeKI	GCWGC	8 cut(s)	674, 1118, 2062, 2087, 2540, 3002, 3101, 3175
ApoI	RAATTY	3 cut(s)	603, 1075, 1858
Asp700I	GAANNNNTTC	2 cut(s)	1661, 2931
AspA2I	CCTAGG	1 cut(s)	2432
AspLEI	GCGC	3 cut(s)	132, 979, 1998
AspS9I	GGNCC	3 cut(s)	864, 1851, 2392
AsuHPI	GGTGA	9 cut(s)	53, 644, 755, 980, 1226, 2288, 2297, 2363, 2689
AsuNHI	GCTAGC	2 cut(s)	1304, 2083
AvaI	CYCGRG	2 cut(s)	26, 2120
AvaII	GGWCC	3 cut(s)	864, 1851, 2392
AvrII	CCTAGG	1 cut(s)	2432
BaeGI	GKGCMC	2 cut(s)	1323, 1331
BaeI	ACNNNNGTAYC	6 cut(s)	553, 586, 798, 831, 3246, 3279
BalI	TGGCCA	2 cut(s)	156, 2185
BamHI	GGATCC	2 cut(s)	923, 2505
BanII	GRGCYC	1 cut(s)	2412
BbsI	GAAGAC	1 cut(s)	204
Bbv12I	GWGCWC	3 cut(s)	1323, 1331, 2412
BbvCI	CCTCAGC	1 cut(s)	1932
BbvI	GCAGC	8 cut(s)	686, 1105, 2049, 2099, 2527, 3014, 3113, 3162
BccI	CCATC	7 cut(s)	195, 1508, 2267, 2306, 2369, 2382, 3409
BceAI	ACGGC	2 cut(s)	719, 3134
BcgI	CGANNNNNNTGC	4 cut(s)	2551, 2585, 3363, 3397
BciT130I	CCWGG	5 cut(s)	834, 1067, 2340, 2364, 3166
BciVI	GTATCC	2 cut(s)	559, 581
BcoDI	GTCTC	5 cut(s)	421, 945, 1334, 3122, 3295
BfaI	CTAG	5 cut(s)	35, 963, 1305, 2084, 2433
BfmI	CTRYAG	4 cut(s)	2541, 3099, 3176, 3304
BfrI	CTTAAG	1 cut(s)	3040
BfuAI	ACCTGC	3 cut(s)	411, 668, 1814
BfuI	GTATCC	2 cut(s)	559, 581
BglII	AGATCT	1 cut(s)	316
BlnI	CCTAGG	1 cut(s)	2432
BlpI	GCTNAGC	3 cut(s)	531, 1280, 2529
Bme1390I	CCNGG	5 cut(s)	834, 1067, 2340, 2364, 3166
Bme18I	GGWCC	3 cut(s)	864, 1851, 2392
BmeT110I	CYCGRG	2 cut(s)	26, 2120
BmgT120I	GGNCC	3 cut(s)	864, 1851, 2392
BmiI	GGNNCC	5 cut(s)	866, 925, 1664, 2393, 2507
BmrFI	CCNGG	5 cut(s)	834, 1067, 2340, 2364, 3166
BmrI	ACTGGG	1 cut(s)	2290
BmsI	GCATC	9 cut(s)	40, 325, 973, 1114, 1531, 1908, 2553, 3193, 3367
BmtI	GCTAGC	2 cut(s)	1308, 2087
BmuI	ACTGGG	1 cut(s)	2290
BpiI	GAAGAC	1 cut(s)	204
BplI	GAGNNNNNCTC	2 cut(s)	2503, 2535
BpmI	CTGGAG	2 cut(s)	1178, 2385
Bpu10I	CCTNAGC	1 cut(s)	1932
Bpu1102I	GCTNAGC	3 cut(s)	531, 1280, 2529
BpuEI	CTTGAG	1 cut(s)	1064
BsaBI	GATNNNNATC	1 cut(s)	2504
BsaHI	GRCGYC	1 cut(s)	3288
BsaJI	CCNNGG	8 cut(s)	74, 219, 370, 860, 2121, 2432, 3164, 3274
BsaWI	WCCGGW	1 cut(s)	2674
Bsc4I	CCNNNNNNNGG	4 cut(s)	241, 580, 1848, 2480
Bse118I	RCCGGY	1 cut(s)	724
Bse1I	ACTGG	9 cut(s)	90, 125, 157, 604, 1324, 1787, 2285, 2485, 2981
Bse3DI	GCAATG	2 cut(s)	1825, 3069
Bse8I	GATNNNNATC	1 cut(s)	2504
BseBI	CCWGG	5 cut(s)	834, 1067, 2340, 2364, 3166
BseDI	CCNNGG	8 cut(s)	74, 219, 370, 860, 2121, 2432, 3164, 3274
BseJI	GATNNNNATC	1 cut(s)	2504
BseLI	CCNNNNNNNGG	4 cut(s)	241, 580, 1848, 2480
BseMI	GCAATG	2 cut(s)	1825, 3069
BseNI	ACTGG	9 cut(s)	90, 125, 157, 604, 1324, 1787, 2285, 2485, 2981
BseRI	GAGGAG	2 cut(s)	2006, 2303
BseSI	GKGCMC	2 cut(s)	1323, 1331
BseXI	GCAGC	8 cut(s)	686, 1105, 2049, 2099, 2527, 3014, 3113, 3162
BseYI	CCCAGC	1 cut(s)	2807
BsgI	GTGCAG	1 cut(s)	3393
Bsh1236I	CGCG	1 cut(s)	130
Bsh1285I	CGRYCG	1 cut(s)	433
BshFI	GGCC	5 cut(s)	156, 728, 1529, 2185, 2547
BsiEI	CGRYCG	1 cut(s)	433
BsiHKAI	GWGCWC	3 cut(s)	1323, 1331, 2412
BsiHKCI	CYCGRG	2 cut(s)	26, 2120
BsiSI	CCGG	2 cut(s)	725, 2675
BslFI	GGGAC	2 cut(s)	259, 2405
BslI	CCNNNNNNNGG	4 cut(s)	241, 580, 1848, 2480
BsmAI	GTCTC	5 cut(s)	421, 945, 1334, 3122, 3295
BsmBI	CGTCTC	2 cut(s)	3122, 3295
BsmFI	GGGAC	2 cut(s)	259, 2405
BsmI	GAATGC	1 cut(s)	2844
BsnI	GGCC	5 cut(s)	156, 728, 1529, 2185, 2547
BsoBI	CYCGRG	2 cut(s)	26, 2120
Bsp1286I	GDGCHC	3 cut(s)	1323, 1331, 2412
Bsp1720I	GCTNAGC	3 cut(s)	531, 1280, 2529
Bsp19I	CCATGG	1 cut(s)	74
BspACI	CCGC	8 cut(s)	281, 451, 769, 1745, 2526, 2538, 2566, 3182
BspANI	GGCC	5 cut(s)	156, 728, 1529, 2185, 2547
BspFNI	CGCG	1 cut(s)	130
BspHI	TCATGA	2 cut(s)	682, 1336
BspLI	GGNNCC	5 cut(s)	866, 925, 1664, 2393, 2507
BspMAI	CTGCAG	3 cut(s)	2545, 3103, 3180
BspMI	ACCTGC	3 cut(s)	411, 668, 1814
BspOI	GCTAGC	2 cut(s)	1308, 2087
BspPI	GGATC	6 cut(s)	231, 918, 931, 1515, 2500, 2513
BspQI	GCTCTTC	1 cut(s)	1812
BspTI	CTTAAG	1 cut(s)	3040
BsrDI	GCAATG	2 cut(s)	1825, 3069
BsrFI	RCCGGY	1 cut(s)	724
BsrI	ACTGG	9 cut(s)	90, 125, 157, 604, 1324, 1787, 2285, 2485, 2981
BssAI	RCCGGY	1 cut(s)	724
BssECI	CCNNGG	8 cut(s)	74, 219, 370, 860, 2121, 2432, 3164, 3274
BssNI	GRCGYC	1 cut(s)	3288
BssT1I	CCWWGG	4 cut(s)	74, 219, 860, 2432
Bst2UI	CCWGG	5 cut(s)	834, 1067, 2340, 2364, 3166
Bst6I	CTCTTC	6 cut(s)	27, 1338, 1812, 2250, 2766, 2951
BstACI	GRCGYC	1 cut(s)	3288
BstAFI	CTTAAG	1 cut(s)	3040
BstAPI	GCANNNNNTGC	1 cut(s)	3175
BstC8I	GCNNGC	9 cut(s)	1306, 1632, 2085, 2486, 2535, 2545, 3000, 3048, 3180
BstDSI	CCRYGG	1 cut(s)	74
BstENI	CCTNNNNNAGG	1 cut(s)	1846
BstFNI	CGCG	1 cut(s)	130
BstHHI	GCGC	3 cut(s)	132, 979, 1998
BstMAI	GTCTC	5 cut(s)	421, 945, 1334, 3122, 3295
BstMCI	CGRYCG	1 cut(s)	433
BstNI	CCWGG	5 cut(s)	834, 1067, 2340, 2364, 3166
BstNSI	RCATGY	3 cut(s)	1292, 2488, 2537
BstSCI	CCNGG	5 cut(s)	832, 1065, 2338, 2362, 3164
BstSFI	CTRYAG	4 cut(s)	2541, 3099, 3176, 3304
BstSLI	GKGCMC	2 cut(s)	1323, 1331
BstUI	CGCG	1 cut(s)	130
BstV1I	GCAGC	8 cut(s)	686, 1105, 2049, 2099, 2527, 3014, 3113, 3162
BstV2I	GAAGAC	1 cut(s)	204
BstX2I	RGATCY	3 cut(s)	316, 923, 2505
BstYI	RGATCY	3 cut(s)	316, 923, 2505
BsuI	GTATCC	2 cut(s)	559, 581
BsuRI	GGCC	5 cut(s)	156, 728, 1529, 2185, 2547
BtgI	CCRYGG	1 cut(s)	74
BtgZI	GCGATG	2 cut(s)	1649, 2576
BtsI	GCAGTG	3 cut(s)	3016, 3096, 3369
BveI	ACCTGC	3 cut(s)	411, 668, 1814
Cac8I	GCNNGC	9 cut(s)	1306, 1632, 2085, 2486, 2535, 2545, 3000, 3048, 3180
CaiI	CAGNNNCTG	1 cut(s)	1909
CciI	TCATGA	2 cut(s)	682, 1336
CfoI	GCGC	3 cut(s)	132, 979, 1998
Cfr10I	RCCGGY	1 cut(s)	724
Cfr13I	GGNCC	3 cut(s)	864, 1851, 2392
CsiI	ACCWGGT	1 cut(s)	832
Csp6I	GTAC	4 cut(s)	70, 2984, 3263, 3345
CspCI	CAANNNNNGTGG	2 cut(s)	1747, 1782
CviQI	GTAC	4 cut(s)	70, 2984, 3263, 3345
DraI	TTTAAA	1 cut(s)	849
DrdI	GACNNNNNNGTC	1 cut(s)	374
DseDI	GACNNNNNNGTC	1 cut(s)	374
EaeI	YGGCCR	2 cut(s)	154, 2183
Eam1104I	CTCTTC	6 cut(s)	27, 1338, 1812, 2250, 2766, 2951
EarI	CTCTTC	6 cut(s)	27, 1338, 1812, 2250, 2766, 2951
EciI	GGCGGA	1 cut(s)	466
Ecl136II	GAGCTC	1 cut(s)	2410
Eco130I	CCWWGG	4 cut(s)	74, 219, 860, 2432
Eco24I	GRGCYC	1 cut(s)	2412
Eco32I	GATATC	1 cut(s)	2682
Eco47I	GGWCC	3 cut(s)	864, 1851, 2392
Eco53kI	GAGCTC	1 cut(s)	2410
Eco57I	CTGAAG	5 cut(s)	867, 2439, 2841, 2975, 3211
Eco88I	CYCGRG	2 cut(s)	26, 2120
EcoICRI	GAGCTC	1 cut(s)	2410
EcoNI	CCTNNNNNAGG	1 cut(s)	1846
EcoO109I	RGGNCCY	2 cut(s)	864, 2392
EcoRI	GAATTC	1 cut(s)	603
EcoRII	CCWGG	5 cut(s)	832, 1065, 2338, 2362, 3164
EcoRV	GATATC	1 cut(s)	2682
EcoT14I	CCWWGG	4 cut(s)	74, 219, 860, 2432
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	1 cut(s)	2412
ErhI	CCWWGG	4 cut(s)	74, 219, 860, 2432
Esp3I	CGTCTC	2 cut(s)	3122, 3295
FalI	AAGNNNNNCTT	8 cut(s)	186, 218, 338, 370, 1858, 1890, 3034, 3066
FaqI	GGGAC	2 cut(s)	259, 2405
FauNDI	CATATG	1 cut(s)	3259
FblI	GTMKAC	1 cut(s)	1420
FriOI	GRGCYC	1 cut(s)	2412
FspBI	CTAG	5 cut(s)	35, 963, 1305, 2084, 2433
FspI	TGCGCA	1 cut(s)	978
GlaI	GCGC	3 cut(s)	131, 978, 1997
GsaI	CCCAGC	1 cut(s)	2811
GsuI	CTGGAG	2 cut(s)	1178, 2385
HaeIII	GGCC	5 cut(s)	156, 728, 1529, 2185, 2547
HapII	CCGG	2 cut(s)	725, 2675
HhaI	GCGC	3 cut(s)	132, 979, 1998
Hin1I	GRCGYC	1 cut(s)	3288
Hin6I	GCGC	3 cut(s)	130, 977, 1996
HinP1I	GCGC	3 cut(s)	130, 977, 1996
HpaII	CCGG	2 cut(s)	725, 2675
HphI	GGTGA	9 cut(s)	53, 644, 755, 980, 1226, 2288, 2297, 2363, 2689
Hpy166II	GTNNAC	6 cut(s)	481, 1217, 1321, 1329, 1421, 2817
Hpy188III	TCNNGA	8 cut(s)	35, 420, 683, 1337, 1511, 1939, 2509, 2637
Hpy8I	GTNNAC	6 cut(s)	481, 1217, 1321, 1329, 1421, 2817
Hpy99I	CGWCG	2 cut(s)	621, 831
HpyCH4IV	ACGT	3 cut(s)	485, 2986, 3288
HpySE526I	ACGT	3 cut(s)	485, 2986, 3288
Hsp92I	GRCGYC	1 cut(s)	3288
HspAI	GCGC	3 cut(s)	130, 977, 1996
LguI	GCTCTTC	1 cut(s)	1812
Lsp1109I	GCAGC	8 cut(s)	686, 1105, 2049, 2099, 2527, 3014, 3113, 3162
LweI	GCATC	9 cut(s)	40, 325, 973, 1114, 1531, 1908, 2553, 3193, 3367
MabI	ACCWGGT	1 cut(s)	832
MaeI	CTAG	5 cut(s)	35, 963, 1305, 2084, 2433
MaeII	ACGT	3 cut(s)	485, 2986, 3288
MfeI	CAATTG	4 cut(s)	379, 1764, 2615, 3029
MflI	RGATCY	3 cut(s)	316, 923, 2505
MhlI	GDGCHC	3 cut(s)	1323, 1331, 2412
MlsI	TGGCCA	2 cut(s)	156, 2185
MluNI	TGGCCA	2 cut(s)	156, 2185
MlyI	GAGTC	3 cut(s)	1096, 2143, 3157
MmeI	TCCRAC	6 cut(s)	576, 640, 2135, 2205, 2498, 2748
Mox20I	TGGCCA	2 cut(s)	156, 2185
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	2 cut(s)	1661, 2931
MscI	TGGCCA	2 cut(s)	156, 2185
MslI	CAYNNNNRTG	1 cut(s)	687
Msp20I	TGGCCA	2 cut(s)	156, 2185
MspA1I	CMGCKG	4 cut(s)	1010, 2090, 2528, 2540
MspCI	CTTAAG	1 cut(s)	3040
MspI	CCGG	2 cut(s)	725, 2675
MspR9I	CCNGG	5 cut(s)	834, 1067, 2340, 2364, 3166
MunI	CAATTG	4 cut(s)	379, 1764, 2615, 3029
Mva1269I	GAATGC	1 cut(s)	2844
MvaI	CCWGG	5 cut(s)	834, 1067, 2340, 2364, 3166
MvnI	CGCG	1 cut(s)	130
NcoI	CCATGG	1 cut(s)	74
NdeI	CATATG	1 cut(s)	3259
NheI	GCTAGC	2 cut(s)	1304, 2083
NlaIV	GGNNCC	5 cut(s)	866, 925, 1664, 2393, 2507
NmuCI	GTSAC	7 cut(s)	494, 586, 841, 968, 2021, 2276, 3241
NsbI	TGCGCA	1 cut(s)	978
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	3 cut(s)	1292, 2488, 2537
PaeI	GCATGC	2 cut(s)	2488, 2537
PagI	TCATGA	2 cut(s)	682, 1336
PciSI	GCTCTTC	1 cut(s)	1812
PctI	GAATGC	1 cut(s)	2844
PdmI	GAANNNNTTC	2 cut(s)	1661, 2931
PfeI	GAWTC	8 cut(s)	640, 664, 746, 1402, 1707, 1751, 2876, 3409
PflFI	GACNNNGTC	2 cut(s)	203, 300
PflMI	CCANNNNNTGG	1 cut(s)	2480
Ple19I	CGATCG	1 cut(s)	433
PleI	GAGTC	3 cut(s)	1096, 2143, 3156
PpsI	GAGTC	3 cut(s)	1096, 2143, 3156
PpuMI	RGGWCCY	2 cut(s)	864, 2392
Psp124BI	GAGCTC	1 cut(s)	2412
Psp5II	RGGWCCY	2 cut(s)	864, 2392
Psp6I	CCWGG	5 cut(s)	832, 1065, 2338, 2362, 3164
PspFI	CCCAGC	1 cut(s)	2807
PspGI	CCWGG	5 cut(s)	832, 1065, 2338, 2362, 3164
PspN4I	GGNNCC	5 cut(s)	866, 925, 1664, 2393, 2507
PspPI	GGNCC	3 cut(s)	864, 1851, 2392
PspPPI	RGGWCCY	2 cut(s)	864, 2392
PstI	CTGCAG	3 cut(s)	2545, 3103, 3180
PstNI	CAGNNNCTG	1 cut(s)	1909
PsuI	RGATCY	3 cut(s)	316, 923, 2505
PsyI	GACNNNGTC	2 cut(s)	203, 300
PvuI	CGATCG	1 cut(s)	433
PvuII	CAGCTG	2 cut(s)	1010, 2090
RsaI	GTAC	4 cut(s)	71, 2985, 3264, 3346
RsaNI	GTAC	4 cut(s)	70, 2984, 3263, 3345
RseI	CAYNNNNRTG	1 cut(s)	687
SacI	GAGCTC	1 cut(s)	2412
SapI	GCTCTTC	1 cut(s)	1812
Sau96I	GGNCC	3 cut(s)	864, 1851, 2392
SchI	GAGTC	3 cut(s)	1096, 2143, 3157
ScrFI	CCNGG	5 cut(s)	834, 1067, 2340, 2364, 3166
SduI	GDGCHC	3 cut(s)	1323, 1331, 2412
SexAI	ACCWGGT	1 cut(s)	832
SfaNI	GCATC	9 cut(s)	40, 325, 973, 1114, 1531, 1908, 2553, 3193, 3367
SfcI	CTRYAG	4 cut(s)	2541, 3099, 3176, 3304
SinI	GGWCC	3 cut(s)	864, 1851, 2392
SmiMI	CAYNNNNRTG	1 cut(s)	687
SmlI	CTYRAG	2 cut(s)	1043, 3040
SmoI	CTYRAG	2 cut(s)	1043, 3040
SphI	GCATGC	2 cut(s)	2488, 2537
SsiI	CCGC	8 cut(s)	281, 451, 769, 1745, 2526, 2538, 2566, 3182
SspI	AATATT	3 cut(s)	1131, 1415, 1735
SspMI	CTAG	5 cut(s)	35, 963, 1305, 2084, 2433
SstI	GAGCTC	1 cut(s)	2412
StyD4I	CCNGG	5 cut(s)	832, 1065, 2338, 2362, 3164
StyI	CCWWGG	4 cut(s)	74, 219, 860, 2432
TaiI	ACGT	3 cut(s)	488, 2989, 3291
TaqI	TCGA	5 cut(s)	826, 1705, 1810, 2625, 3412
TaqII	GACCGA	1 cut(s)	384
TatI	WGTACW	1 cut(s)	3344
TauI	GCSGC	3 cut(s)	2528, 2540, 3185
TfiI	GAWTC	8 cut(s)	640, 664, 746, 1402, 1707, 1751, 2876, 3409
TseFI	GTSAC	7 cut(s)	494, 586, 841, 968, 2021, 2276, 3241
TseI	GCWGC	8 cut(s)	674, 1118, 2062, 2087, 2540, 3002, 3101, 3175
Tsp45I	GTSAC	7 cut(s)	494, 586, 841, 968, 2021, 2276, 3241
TspGWI	ACGGA	5 cut(s)	224, 563, 1039, 1337, 1624
Tth111I	GACNNNGTC	2 cut(s)	203, 300
Van91I	CCANNNNNTGG	1 cut(s)	2480
Vha464I	CTTAAG	1 cut(s)	3040
VneI	GTGCAC	2 cut(s)	1319, 1327
VpaK11BI	GGWCC	3 cut(s)	864, 1851, 2392
XagI	CCTNNNNNAGG	1 cut(s)	1846
XapI	RAATTY	3 cut(s)	603, 1075, 1858
XbaI	TCTAGA	1 cut(s)	34
XceI	RCATGY	3 cut(s)	1292, 2488, 2537
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3121
XmaJI	CCTAGG	1 cut(s)	2432
XmiI	GTMKAC	1 cut(s)	1420
XmnI	GAANNNNTTC	2 cut(s)	1661, 2931
XspI	CTAG	5 cut(s)	35, 963, 1305, 2084, 2433
ZraI	GACGTC	1 cut(s)	3289
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.