Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0000764

Physical Location & Seq

Genomic Coordinates Forward (+)

62080244 .. 62086105

Gene Structure 5862 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00269160.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3450 bp

ATGTTGACCAAGTTCGAGACGAAGAGTAATAGAGTGAAGGGACTGAGCTTCCACACTAAGAGGCCGTGGATCCTCGCGAGTCTGCACAGCGGTGTGATCCAGCTATGGGACTACCGTATGGGGACTCTCATTGATCGATTCGATGAGCACGACGGCCCTGTTCGCGGTGTTCATTTCCACAAATCTCAGCCTCTCTTTGTATCTGGAGGGGACGATTACAAGATTAAAGTTTGGAACTACAAGATGCATAGATGTCTTTTTACTCTTCTCGGGCATCTAGATTATATCCGAACCGTGCAATTTCACCATGAGTACCCATGGATTGTGAGTGCCAGTGATGACCAGACTATTCGTATATGGAACTGGCAGTCACGCACTTGTATTTCAGTTTTGACAGGCCACAATCATTATGTTATGTGTGCCTCATTCCATCCTAAAGAAGACCTTGTCGTATCTGCCTCCTTGGATCAGACTGTCCGTGTTTGGGATATTGGTTCTCTGAAAAAGAAGTCTGTGTCCCCTGCGGATGACATACTGCGACTAAGTCAGATGAACACAGATCTTTTTGGTGGTGTTGATGCTGTTGTTAAGTATGTCTTGGAAGGGCATGACCGAGGTGTCAATTGGGCTTCATTCCACCCCAACCTGCCCCTCATTGTCTCAGGAGCAGACGATCGCCAAGTTAAATTGTGGCGGATGAATGATACTAAGGCTTGGGAAGTGGACACGTTGAGAGGTCACATGAATAATGTTTCATGTGTTATGTTTCATGCTAAGCAGGATATAATTGTATCCAACTCAGAGGACAAAAGTATCCGTGTCTGGGATGTGACAAAGCGAACTGGAATTCAAACTTTCCGACGAGAGCATGATCGCTTTTGGATTCTTTCTTCTCACCCTGAAATGAATCTGTTAGCAGCAGGTCATGACAGTGGGATGATCGTCTTTAAGTTAGAGAGGGAAAGACCGGCCTTTTCCGTCAGTGGTGATTCTCTGTTCTATGCTAAAGACCGCTTTTTGAAGTTCTACGAGTTTTCAACCCAGAGAGATACACAAGTTATTCCAATTCGACGACCTGGTTCTATCACTTTAAATCAAAGTCCAAGGACCCTTTCTTACAGTCCTTCAGAAAATGCAGTTCTTGTTTGCTCAGACTTGGATGGGGGATCCTATGAGTTGTATTTCATACCCAGAGACAGCATTACTAGGGGTGACAGTGCGCAAGATGCTAAGAGAGGTGTTGGTGGTTCAGCTGTGTTTGTAGCACGGAATAGGTTTGCTGTGCTTGAGAAAAGCACAAACTCTGTCCTGGTAAAGAATTTGAGGAATGAGGTTGTTAAAAAGACTCCCCTTCCTTTTGCTGCTGATGCAATATTCTACGCTGGAACAGGTAATTTGCTGTGTAGCAAACATGCCATAATTATTGCTAGCAAGAGGCTTGTGCACCAGTGCACTCTTCATGAGACAATCCGTGTAAAGAGTGGTGGTTGGGATGACAACGGTGTTTTCATTTATTCAACTCTGAATCACATTAAATATTGTCTACCTAATGGAGATAGTGGAATAATCAGAACGCTTGATGTTCCTATCTACATTACGAAAGTTTCTGCAAATACTATCTTTTGCTTGGATCGGGATGGGAAAAATAAGGCCATAGTTATTGATGCCACAGAATACATCTTCAAGTTATCCCTTTTGAAGAAGAAATATGACAATGTTATGAGCATGATAAGGAACTCACAGCTCTGTGGGCAGGCGATGATAGCTTATCTGCAACAGAAGGGGTTCCCCGAAGTTGCCCTCCATTTTGTGAAAGATGAAAGAACTCGATTCAATCTGGCATTAGAGAGTGGGAATATTCAAATTGCGGTTGAATCTGCCACAGCAATTGATGAAAAAGATTACTGGTATAGGTTGGGTGTGGAGGCTCTTCGACAGGGCAATGCAGGTATTGTAGAGTTTGCCTACCAGAGGACCAAAAATTTTGAAAGGCTGTCTTTCCTTTATCTCATAAATGGTAATACACAGAAACTGTCCAAGATGCTGAAAATTGCCGAGGTCAAGAATGATGTCATGGGTCAGTTTCACAATGCACTGTATCTGGGGAATGTTGAGGAGCGCATTAAGATATTGGAGAATGTTGGTCACTTGCCCCTTGCTTATATTACAGCAAAAACTCATGGGCTGCATGATGTTGCCGAAAGGCTAGCAGCTGAGTTGGGGGATAATCTTCCAACTTTGCCCAAGGGTAAAGCACCACCAACTCTTTTGACTCCCCCAACACCAATCATGTGTGGTAGTGATTGGCCACTTCTCAGAGTAATGAGAGGTATATTTGAAGGTGGGTTGGATAATATCGGCAGGGGAGCTGTGGATGAAGAGGATGAGGCTGTTGATGGTGACTGGGGTGAGGAGCTGGACATGGTTGATGTTGATGGGTTACAAAATGGAGTTGTCCTTCCAGGTTTGGAAGGTGAGGAAGTGCCTGGAGAAGATGATGGAGAGGGAGGATGGGACCTTGAAGATTTGGAGCTCCCCCTGAAGCTGACACACCTAGGACTTCTGTTAATACTAATTCATCAGTTTTTGTTGCTCCAACTACTGGCATGCCTGCAACTTGGGATTAAAAACTTCACTCCTTTGAGGCAATTGTTCCTCGATCTTCACTCTGGAAGCCACAGCTATCTACGGGCATTTTCGTCTGCTCCGGTGATATCATTAGCAGTTGAGCGTGGGTGGAATGAAACTACCACGCCAAATGTCAGAAATCCACCAGCACTTGTGTTCAATTTTTCTCAGTTGGAAGAGAAACTCAAAGCTGGTTACAAGGCTACAACTGCTGGGAAGTTCACTGAAGCTCTCAGGCTCTTTCTTAGCATTCTTCATACAATTCCTTTGATTGTTGTTGATTCAAGGAGAGAAGTTGATGAAGTTAAGGAGTTAATTGTAATAGTCAAGGAATATGTTCTGGGATTGCAAATGGAGCTGAAGAGAAGGGAAATGAAAGATAATCCAGTACGTCAGCAGGAGCTTGCAGCTTATTTCACACACTGCAATCTTCAATTGCCTCACTTAAGGCTTGCTTTGCTTAATGCAATGACAGTTTGCTACAAGGCAAAGAACCTTGCCACTGCAGCTAACTTTGCCAGACGGCTATTGGAGACGAACCCCACCATTGAGAGTCAGGCAAAGTTACCCAGGCAAGTGCTGCAGGCGGCAGAGAAGAATATGACTGATGCTTCTCAACTGAACTATGACTTCAGAAATCCATTTGTGACTTGTGGGGCAACATATGTACCAATTTACCGAGGACAGAAGGACGTCTCTTGCCCTTACTGTAGCTCACGGTTTGTGCCAAGCCAAGAAGGGAAGCTCTGTACTGTTTGTGATCTTGCAGTGGTTGGTGCAGATGCTTCGGGGTTGCTATGTTCTCCATCTCAGATTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0005886 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COPI_C Coatomer_WDAD WD40

Protein Analysis

1149

Amino Acids

129.32

Weight (kDa)

7.39

Isoelectric Point (pI)

33.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR36-Utp21_1st	PF25171	2 - 79	2.8e-09	WDR36/Utp21 first beta-propeller
Beta-prop_WDR5	PF25175	3 - 90	1.9e-10	WDR5 beta-propeller domain
WDR55	PF24796	3 - 168	5.9e-13	WDR55
Beta-prop_WDR3_1st	PF25173	4 - 164	2.2e-32	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	4 - 162	9e-16	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	6 - 127	1.4e-17	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	13 - 256	1.4e-14	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd	PF25172	15 - 82	7.5e-06	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	26 - 122	2e-07	G protein beta WD-40 repeat protein
WD40	PF00400	42 - 79	4.6e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	48 - 168	5.8e-09	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	49 - 166	7e-17	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	60 - 168	4.5e-21	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	64 - 167	1.6e-08	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	71 - 166	1.4e-27	WDR5 beta-propeller domain
WD40	PF00400	84 - 121	2.8e-08	WD domain, G-beta repeat
EIF3I	PF24805	88 - 165	3.8e-06	EIF3I
Beta-prop_WDR3_2nd	PF25172	88 - 163	5.5e-09	WDR3 second beta-propeller domain
Beta-prop_EIPR1	PF23609	91 - 162	2.6e-06	EIPR1 beta-propeller
WD40_WDHD1_1st	PF24817	96 - 169	4.1e-13	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	108 - 169	7.1e-13	THOC3 beta-propeller domain
WD40	PF00400	126 - 163	1e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	183 - 305	1.7e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	193 - 317	1.9e-19	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	195 - 314	2.9e-18	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	195 - 307	7.6e-22	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	196 - 290	1.1e-07	TEP-1 second beta-propeller
WD40	PF00400	198 - 231	9.3e-07	WD domain, G-beta repeat
WD40_Gbeta	PF25391	198 - 288	4.1e-06	G protein beta WD-40 repeat protein
EIF3I	PF24805	199 - 287	5e-07	EIF3I
Beta-prop_WDR3_2nd	PF25172	201 - 316	1.4e-09	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	202 - 303	2.7e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	205 - 316	1.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	206 - 316	4.4e-15	THOC3 beta-propeller domain
WDR55	PF24796	207 - 315	3.5e-10	WDR55
WD40_WDHD1_1st	PF24817	211 - 287	3.2e-10	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	212 - 318	2.2e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	223 - 320	1.3e-07	CDC20/Fizzy WD40 domain
WD40	PF00400	241 - 276	1.9e-06	WD domain, G-beta repeat
B-prop_COPA_B_2nd	PF04053	340 - 470	3.5e-23	COPA/B second beta-propeller
B-prop_COPA_B_2nd	PF04053	467 - 544	1.8e-23	COPA/B second beta-propeller
TPR_COPA_B	PF23953	573 - 733	5.5e-20	COPA/B TPR domain
COPI_C	PF06957	776 - 849	4e-06	Coatomer (COPI) alpha subunit C-terminus
COPI_C	PF06957	871 - 1149	9.1e-128	Coatomer (COPI) alpha subunit C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62020 AT2G21390
fragaria_vesca	FvH4_3g10280 FvH4_5g12340 FvH4_5g12340 FvH4_5g12391 FvH4_5g12420
malus_domestica	MD05G1271000.v1.1 MD06G1214600.v1.1 MD10G1248600.v1.1 MD10G1248700.v1.1 MD10G1248800.v1.1 MD10G1249800.v1.1 MD10G1249900.v1.1 MD10G1250100.v1.1 MD10G1250200.v1.1 MD10G1250400.v1.1 MD10G1250600.v1.1 MD10G1250700.v1.1 MD10G1251000.v1.1 MD10G1251300.v1.1 MD10G1251600.v1.1 MD14G1225500.v1.1
prunus_persica	Prupe.4G091800_v2.0.a1 Prupe.4G091800_v2.0.a1 Prupe.5G220000_v2.0.a1 Prupe.5G220000_v2.0.a1
pyrus_communis	pycom05g25340 pycom06g19120 pycom10g20750 pycom14g18670
rosa_chinensis	RchiOBHm_Chr5g0016541 RchiOBHm_Chr7g0185121 RchiOBHm_Chr7g0185201
rosa_laevigata	RLG00000000634 RLG00000000671 RLG00000001958 RLG00000004933 RLG00000032256
rosa_multiflora	Rmu_co8505923.1_g000001 Rmu_co8507327.1_g000001 Rmu_sc0001235.1_g000018 Rmu_sc0004765.1_g000006 Rmu_sc0007192.1_g000011 Rmu_sc0019416.1_g000003 Rmu_ssc0000398.1_g000013
rosa_roxburghii	Rroxscaffold_1G00060340 Rroxscaffold_3G00269160 Rroxscaffold_3G00269190
rosa_rugosa	Rorug05G0032700 Rorug06G0466700 Rorug06G0466700 Rorug06G0466800 Rorug07G0188600
rosa_samantha	Rh4BG061900 Rh4CG068200 Rh4DG290700 Rh5AG127000 Rh5AG459900 Rh5BG125900 Rh5CG098400 Rh5CG138600 Rh5DG126100 Rh7AG076100 Rh7BG071200 Rh7CG072400 Rh7DG071500 Rh7DG071900
rosa_wichuraiana	Rw5G011000 Rw7G005980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	617
AatII	GACGTC	1 cut(s)	3324
Acc16I	TGCGCA	1 cut(s)	1221
Acc36I	ACCTGC	3 cut(s)	654, 911, 1937
AccB7I	CCANNNNNTGG	1 cut(s)	2602
AccI	GTMKAC	1 cut(s)	1543
AccII	CGCG	2 cut(s)	77, 165
AciI	CCGC	7 cut(s)	90, 165, 524, 694, 1012, 1868, 3215
AclWI	GGATC	7 cut(s)	64, 77, 91, 474, 1161, 1174, 1638
AcoI	YGGCCR	1 cut(s)	2306
AcsI	RAATTY	3 cut(s)	846, 1318, 1981
AcuI	CTGAAG	5 cut(s)	1110, 2561, 2874, 3008, 3244
AcyI	GRCGYC	1 cut(s)	3321
AfaI	GTAC	4 cut(s)	314, 3018, 3297, 3379
AfiI	CCNNNNNNNGG	6 cut(s)	106, 164, 484, 823, 1971, 2602
AflII	CTTAAG	1 cut(s)	3073
AflIII	ACRYGT	1 cut(s)	726
AjnI	CCWGG	5 cut(s)	1075, 1308, 2461, 2485, 3197
AjuI	GAANNNNNNNTTGG	2 cut(s)	1096, 1128
AleI	CACNNNNGTG	1 cut(s)	90
Alw21I	GWGCWC	4 cut(s)	150, 1446, 1454, 2535
Alw26I	GTCTC	6 cut(s)	11, 664, 1188, 1457, 3155, 3328
Alw44I	GTGCAC	2 cut(s)	1442, 1450
AlwI	GGATC	7 cut(s)	64, 77, 91, 474, 1161, 1174, 1638
AlwNI	CAGNNNCTG	1 cut(s)	2032
Ama87I	CYCGRG	1 cut(s)	269
AoxI	GGCC	6 cut(s)	62, 154, 397, 969, 1650, 2306
ApaLI	GTGCAC	2 cut(s)	1442, 1450
ApeKI	GCWGC	7 cut(s)	917, 1361, 2185, 2210, 3035, 3134, 3208
ApoI	RAATTY	3 cut(s)	846, 1318, 1981
Asp700I	GAANNNNTTC	2 cut(s)	1784, 2964
AspA2I	CCTAGG	1 cut(s)	2554
AspLEI	GCGC	2 cut(s)	1222, 2121
AspS9I	GGNCC	4 cut(s)	155, 1107, 1974, 2515
AsuHPI	GGTGA	8 cut(s)	296, 887, 998, 1223, 2411, 2420, 2486, 2722
AsuNHI	GCTAGC	2 cut(s)	1427, 2206
AvaI	CYCGRG	1 cut(s)	269
AvaII	GGWCC	3 cut(s)	1107, 1974, 2515
AvrII	CCTAGG	1 cut(s)	2554
BaeGI	GKGCMC	2 cut(s)	1446, 1454
BaeI	ACNNNNGTAYC	6 cut(s)	796, 829, 1041, 1074, 3279, 3312
BalI	TGGCCA	1 cut(s)	2308
BamHI	GGATCC	2 cut(s)	69, 1166
BanII	GRGCYC	1 cut(s)	2535
BbsI	GAAGAC	1 cut(s)	447
Bbv12I	GWGCWC	4 cut(s)	150, 1446, 1454, 2535
BbvI	GCAGC	7 cut(s)	929, 1348, 2172, 2222, 3047, 3146, 3195
BccI	CCATC	8 cut(s)	438, 1154, 1631, 2390, 2429, 2492, 2505, 3442
BceAI	ACGGC	3 cut(s)	49, 169, 3167
BcgI	CGANNNNNNTGC	2 cut(s)	3396, 3430
BciT130I	CCWGG	5 cut(s)	1077, 1310, 2463, 2487, 3199
BciVI	GTATCC	2 cut(s)	802, 824
BcoDI	GTCTC	6 cut(s)	11, 664, 1188, 1457, 3155, 3328
BfaI	CTAG	5 cut(s)	278, 1206, 1428, 2207, 2555
BfmI	CTRYAG	3 cut(s)	3132, 3209, 3337
BfrI	CTTAAG	1 cut(s)	3073
BfuAI	ACCTGC	3 cut(s)	654, 911, 1937
BfuI	GTATCC	2 cut(s)	802, 824
BglII	AGATCT	1 cut(s)	559
BisI	GCNGC	8 cut(s)	918, 1362, 2186, 2211, 3036, 3135, 3209, 3216
BlnI	CCTAGG	1 cut(s)	2554
BlpI	GCTNAGC	1 cut(s)	774
BlsI	GCNGC	8 cut(s)	919, 1363, 2187, 2212, 3037, 3136, 3210, 3217
Bme1390I	CCNGG	5 cut(s)	1077, 1310, 2463, 2487, 3199
Bme18I	GGWCC	3 cut(s)	1107, 1974, 2515
BmeT110I	CYCGRG	1 cut(s)	269
BmgT120I	GGNCC	4 cut(s)	155, 1107, 1974, 2515
BmiI	GGNNCC	5 cut(s)	71, 1109, 1168, 1787, 2516
BmrFI	CCNGG	5 cut(s)	1077, 1310, 2463, 2487, 3199
BmrI	ACTGGG	1 cut(s)	2413
BmsI	GCATC	9 cut(s)	234, 283, 568, 1216, 1357, 1654, 2031, 3226, 3400
BmtI	GCTAGC	2 cut(s)	1431, 2210
BmuI	ACTGGG	1 cut(s)	2413
BpiI	GAAGAC	1 cut(s)	447
BpmI	CTGGAG	2 cut(s)	225, 2508
Bpu1102I	GCTNAGC	1 cut(s)	774
BpuEI	CTTGAG	1 cut(s)	1307
Bsa29I	ATCGAT	1 cut(s)	136
BsaHI	GRCGYC	1 cut(s)	3321
BsaWI	WCCGGW	1 cut(s)	2707
Bsc4I	CCNNNNNNNGG	6 cut(s)	106, 164, 484, 823, 1971, 2602
Bse118I	RCCGGY	1 cut(s)	967
Bse1I	ACTGG	8 cut(s)	333, 368, 847, 1447, 1910, 2408, 2607, 3014
Bse3DI	GCAATG	2 cut(s)	1948, 3102
BseBI	CCWGG	5 cut(s)	1077, 1310, 2463, 2487, 3199
BseCI	ATCGAT	1 cut(s)	136
BseLI	CCNNNNNNNGG	6 cut(s)	106, 164, 484, 823, 1971, 2602
BseMI	GCAATG	2 cut(s)	1948, 3102
BseMII	CTCAG	9 cut(s)	35, 200, 675, 813, 1164, 2205, 2329, 2810, 2875
BseNI	ACTGG	8 cut(s)	333, 368, 847, 1447, 1910, 2408, 2607, 3014
BseRI	GAGGAG	2 cut(s)	2129, 2426
BseSI	GKGCMC	2 cut(s)	1446, 1454
BseXI	GCAGC	7 cut(s)	929, 1348, 2172, 2222, 3047, 3146, 3195
BseYI	CCCAGC	1 cut(s)	2840
BsgI	GTGCAG	2 cut(s)	68, 3426
Bsh1236I	CGCG	2 cut(s)	77, 165
Bsh1285I	CGRYCG	1 cut(s)	676
BshFI	GGCC	6 cut(s)	64, 156, 399, 971, 1652, 2308
BshVI	ATCGAT	1 cut(s)	136
BsiEI	CGRYCG	1 cut(s)	676
BsiHKAI	GWGCWC	4 cut(s)	150, 1446, 1454, 2535
BsiHKCI	CYCGRG	1 cut(s)	269
BsiSI	CCGG	2 cut(s)	968, 2708
BslFI	GGGAC	6 cut(s)	54, 122, 136, 224, 502, 2528
BslI	CCNNNNNNNGG	6 cut(s)	106, 164, 484, 823, 1971, 2602
BsmAI	GTCTC	6 cut(s)	11, 664, 1188, 1457, 3155, 3328
BsmBI	CGTCTC	3 cut(s)	11, 3155, 3328
BsmFI	GGGAC	6 cut(s)	54, 122, 136, 224, 502, 2528
BsmI	GAATGC	1 cut(s)	2877
BsnI	GGCC	6 cut(s)	64, 156, 399, 971, 1652, 2308
BsoBI	CYCGRG	1 cut(s)	269
Bsp1286I	GDGCHC	4 cut(s)	150, 1446, 1454, 2535
Bsp1720I	GCTNAGC	1 cut(s)	774
Bsp19I	CCATGG	1 cut(s)	317
Bsp68I	TCGCGA	1 cut(s)	77
BspACI	CCGC	7 cut(s)	90, 165, 524, 694, 1012, 1868, 3215
BspANI	GGCC	6 cut(s)	64, 156, 399, 971, 1652, 2308
BspCNI	CTCAG	9 cut(s)	36, 199, 674, 812, 1163, 2206, 2328, 2809, 2874
BspDI	ATCGAT	1 cut(s)	136
BspFNI	CGCG	2 cut(s)	77, 165
BspHI	TCATGA	2 cut(s)	925, 1459
BspLI	GGNNCC	5 cut(s)	71, 1109, 1168, 1787, 2516
BspMAI	CTGCAG	2 cut(s)	3136, 3213
BspMI	ACCTGC	3 cut(s)	654, 911, 1937
BspOI	GCTAGC	2 cut(s)	1431, 2210
BspPI	GGATC	7 cut(s)	64, 77, 91, 474, 1161, 1174, 1638
BspQI	GCTCTTC	1 cut(s)	1935
BspTI	CTTAAG	1 cut(s)	3073
BsrDI	GCAATG	2 cut(s)	1948, 3102
BsrFI	RCCGGY	1 cut(s)	967
BsrI	ACTGG	8 cut(s)	333, 368, 847, 1447, 1910, 2408, 2607, 3014
BssAI	RCCGGY	1 cut(s)	967
BssNI	GRCGYC	1 cut(s)	3321
BssT1I	CCWWGG	5 cut(s)	317, 462, 1103, 2244, 2554
Bst2UI	CCWGG	5 cut(s)	1077, 1310, 2463, 2487, 3199
Bst6I	CTCTTC	7 cut(s)	17, 270, 1461, 1935, 2373, 2799, 2984
BstACI	GRCGYC	1 cut(s)	3321
BstAFI	CTTAAG	1 cut(s)	3073
BstAPI	GCANNNNNTGC	1 cut(s)	3208
BstC8I	GCNNGC	8 cut(s)	1429, 1755, 2208, 2608, 2612, 3033, 3081, 3213
BstDSI	CCRYGG	2 cut(s)	65, 317
BstENI	CCTNNNNNAGG	1 cut(s)	1969
BstFNI	CGCG	2 cut(s)	77, 165
BstHHI	GCGC	2 cut(s)	1222, 2121
BstMAI	GTCTC	6 cut(s)	11, 664, 1188, 1457, 3155, 3328
BstMCI	CGRYCG	1 cut(s)	676
BstNI	CCWGG	5 cut(s)	1077, 1310, 2463, 2487, 3199
BstNSI	RCATGY	2 cut(s)	1415, 2610
BstSCI	CCNGG	5 cut(s)	1075, 1308, 2461, 2485, 3197
BstSFI	CTRYAG	3 cut(s)	3132, 3209, 3337
BstSLI	GKGCMC	2 cut(s)	1446, 1454
BstUI	CGCG	2 cut(s)	77, 165
BstV1I	GCAGC	7 cut(s)	929, 1348, 2172, 2222, 3047, 3146, 3195
BstV2I	GAAGAC	1 cut(s)	447
BstX2I	RGATCY	3 cut(s)	69, 559, 1166
BstYI	RGATCY	3 cut(s)	69, 559, 1166
Bsu15I	ATCGAT	1 cut(s)	136
BsuI	GTATCC	2 cut(s)	802, 824
BsuRI	GGCC	6 cut(s)	64, 156, 399, 971, 1652, 2308
BsuTUI	ATCGAT	1 cut(s)	136
BtgI	CCRYGG	2 cut(s)	65, 317
BtgZI	GCGATG	1 cut(s)	1772
BtsI	GCAGTG	3 cut(s)	3049, 3129, 3402
BtuMI	TCGCGA	1 cut(s)	77
BveI	ACCTGC	3 cut(s)	654, 911, 1937
Cac8I	GCNNGC	8 cut(s)	1429, 1755, 2208, 2608, 2612, 3033, 3081, 3213
CaiI	CAGNNNCTG	1 cut(s)	2032
CciI	TCATGA	2 cut(s)	925, 1459
CfoI	GCGC	2 cut(s)	1222, 2121
Cfr10I	RCCGGY	1 cut(s)	967
Cfr13I	GGNCC	4 cut(s)	155, 1107, 1974, 2515
ClaI	ATCGAT	1 cut(s)	136
CsiI	ACCWGGT	1 cut(s)	1075
Csp6I	GTAC	4 cut(s)	313, 3017, 3296, 3378
CspCI	CAANNNNNGTGG	2 cut(s)	1870, 1905
CviQI	GTAC	4 cut(s)	313, 3017, 3296, 3378
DraI	TTTAAA	1 cut(s)	1092
DrdI	GACNNNNNNGTC	1 cut(s)	617
DseDI	GACNNNNNNGTC	1 cut(s)	617
EaeI	YGGCCR	1 cut(s)	2306
Eam1104I	CTCTTC	7 cut(s)	17, 270, 1461, 1935, 2373, 2799, 2984
EarI	CTCTTC	7 cut(s)	17, 270, 1461, 1935, 2373, 2799, 2984
EciI	GGCGGA	1 cut(s)	709
Ecl136II	GAGCTC	1 cut(s)	2533
Eco130I	CCWWGG	5 cut(s)	317, 462, 1103, 2244, 2554
Eco24I	GRGCYC	1 cut(s)	2535
Eco32I	GATATC	1 cut(s)	2715
Eco47I	GGWCC	3 cut(s)	1107, 1974, 2515
Eco53kI	GAGCTC	1 cut(s)	2533
Eco57I	CTGAAG	5 cut(s)	1110, 2561, 2874, 3008, 3244
Eco88I	CYCGRG	1 cut(s)	269
EcoICRI	GAGCTC	1 cut(s)	2533
EcoNI	CCTNNNNNAGG	1 cut(s)	1969
EcoO109I	RGGNCCY	2 cut(s)	1107, 2515
EcoRI	GAATTC	1 cut(s)	846
EcoRII	CCWGG	5 cut(s)	1075, 1308, 2461, 2485, 3197
EcoRV	GATATC	1 cut(s)	2715
EcoT14I	CCWWGG	5 cut(s)	317, 462, 1103, 2244, 2554
EcoT22I	ATGCAT	1 cut(s)	249
EcoT38I	GRGCYC	1 cut(s)	2535
ErhI	CCWWGG	5 cut(s)	317, 462, 1103, 2244, 2554
Esp3I	CGTCTC	3 cut(s)	11, 3155, 3328
FalI	AAGNNNNNCTT	8 cut(s)	429, 461, 581, 613, 1981, 2013, 3067, 3099
FaqI	GGGAC	6 cut(s)	54, 122, 136, 224, 502, 2528
FauNDI	CATATG	1 cut(s)	3292
FblI	GTMKAC	1 cut(s)	1543
Fnu4HI	GCNGC	8 cut(s)	918, 1362, 2186, 2211, 3036, 3135, 3209, 3216
FriOI	GRGCYC	1 cut(s)	2535
Fsp4HI	GCNGC	8 cut(s)	918, 1362, 2186, 2211, 3036, 3135, 3209, 3216
FspBI	CTAG	5 cut(s)	278, 1206, 1428, 2207, 2555
FspI	TGCGCA	1 cut(s)	1221
GlaI	GCGC	2 cut(s)	1221, 2120
GluI	GCNGC	8 cut(s)	918, 1362, 2186, 2211, 3036, 3135, 3209, 3216
GsaI	CCCAGC	1 cut(s)	2844
GsuI	CTGGAG	2 cut(s)	225, 2508
HaeIII	GGCC	6 cut(s)	64, 156, 399, 971, 1652, 2308
HapII	CCGG	2 cut(s)	968, 2708
HhaI	GCGC	2 cut(s)	1222, 2121
Hin1I	GRCGYC	1 cut(s)	3321
Hin6I	GCGC	2 cut(s)	1220, 2119
HinP1I	GCGC	2 cut(s)	1220, 2119
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HpaII	CCGG	2 cut(s)	968, 2708
HphI	GGTGA	8 cut(s)	296, 887, 998, 1223, 2411, 2420, 2486, 2722
Hpy166II	GTNNAC	6 cut(s)	6, 724, 1444, 1452, 1544, 2850
Hpy8I	GTNNAC	6 cut(s)	6, 724, 1444, 1452, 1544, 2850
Hpy99I	CGWCG	3 cut(s)	155, 864, 1074
HpyCH4IV	ACGT	3 cut(s)	728, 3019, 3321
HpySE526I	ACGT	3 cut(s)	728, 3019, 3321
Hsp92I	GRCGYC	1 cut(s)	3321
HspAI	GCGC	2 cut(s)	1220, 2119
LguI	GCTCTTC	1 cut(s)	1935
Lsp1109I	GCAGC	7 cut(s)	929, 1348, 2172, 2222, 3047, 3146, 3195
LweI	GCATC	9 cut(s)	234, 283, 568, 1216, 1357, 1654, 2031, 3226, 3400
MabI	ACCWGGT	1 cut(s)	1075
MaeI	CTAG	5 cut(s)	278, 1206, 1428, 2207, 2555
MaeII	ACGT	3 cut(s)	728, 3019, 3321
MfeI	CAATTG	4 cut(s)	622, 1887, 2648, 3062
MflI	RGATCY	3 cut(s)	69, 559, 1166
MhlI	GDGCHC	4 cut(s)	150, 1446, 1454, 2535
MlsI	TGGCCA	1 cut(s)	2308
MluNI	TGGCCA	1 cut(s)	2308
MlyI	GAGTC	5 cut(s)	88, 118, 1339, 2266, 3190
MmeI	TCCRAC	6 cut(s)	819, 883, 2258, 2328, 2620, 2781
Mox20I	TGGCCA	1 cut(s)	2308
Mph1103I	ATGCAT	1 cut(s)	249
MroXI	GAANNNNTTC	2 cut(s)	1784, 2964
MscI	TGGCCA	1 cut(s)	2308
MslI	CAYNNNNRTG	2 cut(s)	90, 930
Msp20I	TGGCCA	1 cut(s)	2308
MspA1I	CMGCKG	3 cut(s)	90, 1253, 2213
MspCI	CTTAAG	1 cut(s)	3073
MspI	CCGG	2 cut(s)	968, 2708
MspR9I	CCNGG	5 cut(s)	1077, 1310, 2463, 2487, 3199
MunI	CAATTG	4 cut(s)	622, 1887, 2648, 3062
Mva1269I	GAATGC	1 cut(s)	2877
MvaI	CCWGG	5 cut(s)	1077, 1310, 2463, 2487, 3199
MvnI	CGCG	2 cut(s)	77, 165
NcoI	CCATGG	1 cut(s)	317
NdeI	CATATG	1 cut(s)	3292
NheI	GCTAGC	2 cut(s)	1427, 2206
NlaIV	GGNNCC	5 cut(s)	71, 1109, 1168, 1787, 2516
NmeAIII	GCCGAG	1 cut(s)	2080
NmuCI	GTSAC	7 cut(s)	369, 737, 829, 1211, 2144, 2399, 3274
NruI	TCGCGA	1 cut(s)	77
NsbI	TGCGCA	1 cut(s)	1221
NsiI	ATGCAT	1 cut(s)	249
NspI	RCATGY	2 cut(s)	1415, 2610
OliI	CACNNNNGTG	1 cut(s)	90
PaeI	GCATGC	1 cut(s)	2610
PagI	TCATGA	2 cut(s)	925, 1459
PciSI	GCTCTTC	1 cut(s)	1935
PcsI	WCGNNNNNNNCGW	1 cut(s)	147
PctI	GAATGC	1 cut(s)	2877
PdmI	GAANNNNTTC	2 cut(s)	1784, 2964
PfeI	GAWTC	9 cut(s)	138, 883, 907, 989, 1525, 1830, 1874, 2909, 3442
PflFI	GACNNNGTC	2 cut(s)	446, 543
PflMI	CCANNNNNTGG	1 cut(s)	2602
PkrI	GCNGC	8 cut(s)	919, 1363, 2187, 2212, 3037, 3136, 3210, 3217
Ple19I	CGATCG	1 cut(s)	676
PleI	GAGTC	5 cut(s)	87, 118, 1339, 2266, 3189
PpsI	GAGTC	5 cut(s)	87, 118, 1339, 2266, 3189
PpuMI	RGGWCCY	2 cut(s)	1107, 2515
Psp124BI	GAGCTC	1 cut(s)	2535
Psp5II	RGGWCCY	2 cut(s)	1107, 2515
Psp6I	CCWGG	5 cut(s)	1075, 1308, 2461, 2485, 3197
PspFI	CCCAGC	1 cut(s)	2840
PspGI	CCWGG	5 cut(s)	1075, 1308, 2461, 2485, 3197
PspN4I	GGNNCC	5 cut(s)	71, 1109, 1168, 1787, 2516
PspPI	GGNCC	4 cut(s)	155, 1107, 1974, 2515
PspPPI	RGGWCCY	2 cut(s)	1107, 2515
PstI	CTGCAG	2 cut(s)	3136, 3213
PstNI	CAGNNNCTG	1 cut(s)	2032
PsuI	RGATCY	3 cut(s)	69, 559, 1166
PsyI	GACNNNGTC	2 cut(s)	446, 543
PvuI	CGATCG	1 cut(s)	676
PvuII	CAGCTG	2 cut(s)	1253, 2213
RruI	TCGCGA	1 cut(s)	77
RsaI	GTAC	4 cut(s)	314, 3018, 3297, 3379
RsaNI	GTAC	4 cut(s)	313, 3017, 3296, 3378
RseI	CAYNNNNRTG	2 cut(s)	90, 930
SacI	GAGCTC	1 cut(s)	2535
SapI	GCTCTTC	1 cut(s)	1935
SatI	GCNGC	8 cut(s)	918, 1362, 2186, 2211, 3036, 3135, 3209, 3216
Sau96I	GGNCC	4 cut(s)	155, 1107, 1974, 2515
SchI	GAGTC	5 cut(s)	88, 118, 1339, 2266, 3190
ScrFI	CCNGG	5 cut(s)	1077, 1310, 2463, 2487, 3199
SduI	GDGCHC	4 cut(s)	150, 1446, 1454, 2535
SexAI	ACCWGGT	1 cut(s)	1075
SfaNI	GCATC	9 cut(s)	234, 283, 568, 1216, 1357, 1654, 2031, 3226, 3400
SfcI	CTRYAG	3 cut(s)	3132, 3209, 3337
SinI	GGWCC	3 cut(s)	1107, 1974, 2515
SmiMI	CAYNNNNRTG	2 cut(s)	90, 930
SmlI	CTYRAG	2 cut(s)	1286, 3073
SmoI	CTYRAG	2 cut(s)	1286, 3073
SphI	GCATGC	1 cut(s)	2610
SsiI	CCGC	7 cut(s)	90, 165, 524, 694, 1012, 1868, 3215
SspI	AATATT	3 cut(s)	1374, 1538, 1858
SspMI	CTAG	5 cut(s)	278, 1206, 1428, 2207, 2555
SstI	GAGCTC	1 cut(s)	2535
StyD4I	CCNGG	5 cut(s)	1075, 1308, 2461, 2485, 3197
StyI	CCWWGG	5 cut(s)	317, 462, 1103, 2244, 2554
TaiI	ACGT	3 cut(s)	731, 3022, 3324
TaqI	TCGA	8 cut(s)	15, 136, 141, 1069, 1828, 1933, 2658, 3445
TaqII	GACCGA	1 cut(s)	627
TatI	WGTACW	1 cut(s)	3377
TauI	GCSGC	1 cut(s)	3218
TfiI	GAWTC	9 cut(s)	138, 883, 907, 989, 1525, 1830, 1874, 2909, 3442
TseFI	GTSAC	7 cut(s)	369, 737, 829, 1211, 2144, 2399, 3274
TseI	GCWGC	7 cut(s)	917, 1361, 2185, 2210, 3035, 3134, 3208
Tsp45I	GTSAC	7 cut(s)	369, 737, 829, 1211, 2144, 2399, 3274
TspGWI	ACGGA	5 cut(s)	467, 806, 967, 1282, 1460
Tth111I	GACNNNGTC	2 cut(s)	446, 543
Van91I	CCANNNNNTGG	1 cut(s)	2602
Vha464I	CTTAAG	1 cut(s)	3073
VneI	GTGCAC	2 cut(s)	1442, 1450
VpaK11BI	GGWCC	3 cut(s)	1107, 1974, 2515
XagI	CCTNNNNNAGG	1 cut(s)	1969
XapI	RAATTY	3 cut(s)	846, 1318, 1981
XbaI	TCTAGA	1 cut(s)	277
XceI	RCATGY	2 cut(s)	1415, 2610
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3154
XmaJI	CCTAGG	1 cut(s)	2554
XmiI	GTMKAC	1 cut(s)	1543
XmnI	GAANNNNTTC	2 cut(s)	1784, 2964
XspI	CTAG	5 cut(s)	278, 1206, 1428, 2207, 2555
ZraI	GACGTC	1 cut(s)	3322
Zsp2I	ATGCAT	1 cut(s)	249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.