Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0000764

Physical Location & Seq

Genomic Coordinates Reverse (-)

34722618 .. 34728291

Gene Structure 5674 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1214600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3534 bp

ATGGGGACGCTCATTGATCGATTCGACGAGCACGATGGGCCTGTTCGCGGCGTCCACTTTCACAAATCTCAGCCCCTCTTTGTCTCTGGAGGGGATGATTATAAGATTAAAGTTTGGAACTATAAGATGCATAGGTGTCTTTTTACTCTTCTCGGGCACCTTGATTATATCCGTACCGTGCAGTTTCACCACGAATACCCGTGGATTGTGAGTGCCAGTGATGACCAGACCATTCGTATATGGAATTGGCAGTCACGTACTTGTATTTCAGTGTTGACAGGGCACAATCATTATGTCATGTGTGCCTCATTCCATCCTAAAGAAGACCTTGTTGTATCTGCCTCTCTAGATCAGACTGTCCGAGTTTGGGATATTGGTTCCCTGAAAAAGAAGACCGTATCACCGGCAGATGACATTCTGCGACTAAGTCAGATGAACACAGATCTTTTTGGCGGTGTTGATACTGTTGTTAAGTATGTCTTGGAAGGGCATGATCGTGGTGTCAATTGGGCCTCATTCCACCCCAACCTGCCTCTGATTGTCTCAGGAGCAGATGATCGCCAAGTTAAATTGTGGCGAATGAATGATACAAAGGCTTGGGAAGTGGATACATTGAGAGGGCACATGAATAATGTTTCATGTGTCATGTTTCATGCCAAACAGGACATAATTGTATCTAACTCGGAGGACAAAAGTATACGTGTCTGGGATGTAACGAAGCGGACTGGAATTCAAACTTTCCGAAGAGAGCATGACCGTTTTTGGATTCTTTCATCTCACCCTGAAATGAATCTGTTGGCAGCTGGTCATGACAGTGGCATGATTGTATTTAAGCTAGAGAGGGAACGACCAGCCTTTGCAGTGAGTGGTAGTTCTCTGTTCTACGTTAAAGATCGCTTTTTGCGGTACTATGAGTTTTCAACCCAAAGAGACACACAAGTTATTCCAATTCGACGCCCTGGTTCCACCACTTTAAATCAAAGTCCAAGGACTCTTTCTTACAGTCCTACAGAAAATGCAGTTCTTATTTCCTCAGACTTGGATGGGGGATCTTATGAATTGTATTTGATACCCAAAGACAGCATTAGTAGGGGTGATAGTCTGCAAGATGCAAAGAGAGGTGTTGGAGGTTCAGCTGTGTTTATTGCACGGAATAGGTTCGCTGTGCTTGACAAAATCAACAACCAAGTCTTGATTAAGAATATGGCGAATGAGGCTGGTAAAAAGATCCCCCTTCCTTTTGCTGCTGACGCAATATTCTATGCTGGATCAAATAATTTGCTGTGTAGAGTAGAAGACAAAGTGGTTATATTTGATCTCCAGCAGAAGATTATACTTGGTGAACTTCAAACCCCCTTCATCAAGTATGTTGTTTGGTCCAATGACATGGAAAGTGTTGCCTTGCTCAGCAAACATGCCATTATCATTGCCAACAAAAAGCTTGTGCATCAGTGCACTCTTCATGAGACAATCCGTGTGAAGAGTGGTGGATGGGATGACAATGGTGTTTTCATTTATACGACTCTAAATCACATAAAATATTGCCTTCCTAATGGAGACAGTGGAATAATCAGAACCCTAGATGTTCCTATTTACATTACGAAGGTTTCTGGAAATACTATCTTCTGCTTGGATAGGGATGGGAAAAACAGGGCCATAGTTATTGATGCTACGGAATACATTTTCAAGTTATCTCTATTGAAGAAGAGATATGACCATGTAATGAGCATGATAAGGAACTCACAGCTATGTGGGCAGGCAATGATTGCTTATCTGCAACAGAAGGGGTTTCCTGAAGTTGCACTCCATTTTGTGAAAGATGAAAGAACCAGATTCAACCTGGCTCTGGAGAGTGGGAATATTCAAATTGCAGTTGCATCCGCTACAGCAATCGATGAAAAAGACTACTGGTATAGGTTGGGGGTGGAGGCTCTTCGCCAAGGCAATGCGGGTATTGTGGAGTATGCCTACCAGAAGACTAAAAATTTTGAGAGGCTATCTTTCCTTTATCTAATTACCGGTAATATGGAAAAACTGTCCAAGATGCTGAAAATTGCTGAGGTTAAGAATGATGTCATGGGTCAGTTCCACAATGCACTGTATCTAGGCAATGTCAAGGAGCGTATTAAGATCTTGGAGAATGTTGGCCACTTGCCACTTGCTTACATCACAGCTTCAGTTCACGGCCTGCACGATGTTGCTGAAAGGCTATCTGCAGAGTTGGGAGAAAATGTTCCGACATTGCCGCAGGGGAAAGTGCCCACTCTTTTAATGCCCCCTACCCCAATTGTGTGTGGCGGTGATTGGCCTCTTCTTAGAGTCATGAGAGGTATTTTTGAAGGTGGATTGGATAATGTTGGCAGGGGAGCTGCGGATGAAGAGGATGAGGCTGTTGATGGTGATTGGGGTGAGGAGCTGGACATGGTTGACGTTGATGGCTTGCAAAATGGAGATGGCACCGCAGGATTAGAAGATGAGGAAACAGGTGAAGGACATGAGGAAGAGGAAGGATGGGACCTTGAAGATTTGGAGCTTCCCCCTGAAGCTGACACACCAAGGGCTTCTGTAAATGCTAGTTCGTTTGTTGCTCCAACTACTGGCATGCCTGTAAGCCAGATTTGGACCCAGAGGTCATCTCTTGCCGCTGAACATGCAGCAGCTGGCAATTTTGACACTGCTATGCGTTTACTGAAAAGGCAACTTGGATTCAAAAACTTTGCTCTTTTAAAACCAATGTTTCTTGATCTTCACACTGGCAGCCACAGCTATCTTCCTGCATTTTCATCTGCACCGGTGATATCTCTAGCGGTTGAGCGGGGGTGGAATGTATCTGCTACCCCAAATGTGAGAGGTCCACCAGCACTCGTGTTCAATTTTTCCCAGTTGGAAGAGAAACTGAAAGCTGGTTACAAGGCTACCACAAATGGGAAGCTCGCCGACGCTCTGAAGATCTTTCTGAGCATTCTTCACACAATTCCTCTGATTGTTGTTGATTCTAGGAGGGAAGTCGATGAAGTCAAGGAGTTAATTATAATAGTCAAAGAGTATGTTCTGGGATTACAAATCGAGCTGAAGAGAAGGGAAATGAAAGATGATCCAAAACGGGAGCAGGAGCTTGCAGCTTATTTCACGCACTGCAATCTTCAAACACCTCACTTACGGCTTGCCTTGAATAGTGCACTGACTGTTTGCTATAAGGCCGGGAACATTGCTACTGCAGCTAACTTTGCCAGGCGGCTGTTGGAAACAAACCCCACCAATGAGAGTCTAGCAAAGAAAGCCCGGCAAGTGCTGCAGGCTGCAGAAAGGAATATGACTGATAAATCTCAACTGAACTATGATTTCAGAAACCCATTTGTGATTTGTGGGGCAACATATGTACCAATTTACCGAGGACAGAAGGATGTCTCTTGCCCATATTGTAGCTCACGGTTTGTGGCAACTCAAGAAGGGCAGCTCTGTACTGTTTGTGATCTTGCAGTGGTTGGGGCAGATGCTTCTGGGTTGCTATGTTCTCCTTCTCAGGTTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0005886 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COPI_C Coatomer_WDAD WD40

Protein Analysis

1178

Amino Acids

131.84

Weight (kDa)

6.36

Isoelectric Point (pI)

31.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40	PF00400	3 - 40	4.8e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	5 - 88	5.3e-12	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	6 - 123	2.7e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	7 - 125	1.7e-26	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	9 - 127	1.4e-31	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	10 - 129	6.5e-09	CAF1B/HIR1 beta-propeller domain
WDR55	PF24796	10 - 128	2.6e-10	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	10 - 127	6.8e-17	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML	PF23409	10 - 124	2.5e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd	PF25172	11 - 124	9.7e-14	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	13 - 91	2.7e-10	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	16 - 127	1.4e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	16 - 129	1.5e-21	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	24 - 128	1.5e-08	TEP-1 second beta-propeller
Beta-prop_SCAP	PF24017	27 - 127	7.8e-06	SCAP Beta-propeller
WD40	PF00400	45 - 82	2.9e-08	WD domain, G-beta repeat
EIF3I	PF24805	49 - 127	3.6e-06	EIF3I
Beta-prop_EIPR1	PF23609	52 - 123	2.7e-06	EIPR1 beta-propeller
WD40_WDHD1_1st	PF24817	57 - 130	4.7e-13	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	69 - 130	6e-13	THOC3 beta-propeller domain
WD40	PF00400	87 - 124	1.1e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	144 - 267	1.2e-07	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	154 - 275	3e-18	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	154 - 278	1.9e-19	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	156 - 268	7.9e-22	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	157 - 251	1.1e-07	TEP-1 second beta-propeller
WD40	PF00400	158 - 192	8.6e-07	WD domain, G-beta repeat
WD40_Gbeta	PF25391	158 - 249	3.2e-06	G protein beta WD-40 repeat protein
EIF3I	PF24805	160 - 248	5.3e-07	EIF3I
Beta-prop_WDR3_2nd	PF25172	161 - 278	1.1e-09	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	163 - 264	3.3e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	166 - 277	1.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	167 - 277	3.2e-15	THOC3 beta-propeller domain
WDR55	PF24796	168 - 276	4e-10	WDR55
WD40_WDHD1_1st	PF24817	172 - 247	3.8e-10	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	173 - 280	1.7e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	184 - 279	1.4e-07	CDC20/Fizzy WD40 domain
WD40	PF00400	202 - 237	1.9e-06	WD domain, G-beta repeat
B-prop_COPA_B_2nd	PF04053	301 - 545	9.3e-68	COPA/B second beta-propeller
TPR_COPA_B	PF23953	586 - 732	6.8e-19	COPA/B TPR domain
COPI_C	PF06957	776 - 1177	3.3e-165	Coatomer (COPI) alpha subunit C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62020 AT2G21390
fragaria_vesca	FvH4_3g10280 FvH4_5g12340 FvH4_5g12340 FvH4_5g12391 FvH4_5g12420
malus_domestica	MD05G1271000.v1.1 MD06G1214600.v1.1 MD10G1248600.v1.1 MD10G1248700.v1.1 MD10G1248800.v1.1 MD10G1249800.v1.1 MD10G1249900.v1.1 MD10G1250100.v1.1 MD10G1250200.v1.1 MD10G1250400.v1.1 MD10G1250600.v1.1 MD10G1250700.v1.1 MD10G1251000.v1.1 MD10G1251300.v1.1 MD10G1251600.v1.1 MD14G1225500.v1.1
prunus_persica	Prupe.4G091800_v2.0.a1 Prupe.4G091800_v2.0.a1 Prupe.5G220000_v2.0.a1 Prupe.5G220000_v2.0.a1
pyrus_communis	pycom05g25340 pycom06g19120 pycom10g20750 pycom14g18670
rosa_chinensis	RchiOBHm_Chr5g0016541 RchiOBHm_Chr7g0185121 RchiOBHm_Chr7g0185201
rosa_laevigata	RLG00000000634 RLG00000000671 RLG00000001958 RLG00000004933 RLG00000032256
rosa_multiflora	Rmu_co8505923.1_g000001 Rmu_co8507327.1_g000001 Rmu_sc0001235.1_g000018 Rmu_sc0004765.1_g000006 Rmu_sc0007192.1_g000011 Rmu_sc0019416.1_g000003 Rmu_ssc0000398.1_g000013
rosa_roxburghii	Rroxscaffold_1G00060340 Rroxscaffold_3G00269160 Rroxscaffold_3G00269190
rosa_rugosa	Rorug05G0032700 Rorug06G0466700 Rorug06G0466700 Rorug06G0466800 Rorug07G0188600
rosa_samantha	Rh4BG061900 Rh4CG068200 Rh4DG290700 Rh5AG127000 Rh5AG459900 Rh5BG125900 Rh5CG098400 Rh5CG138600 Rh5DG126100 Rh7AG076100 Rh7BG071200 Rh7CG072400 Rh7DG071500 Rh7DG071900
rosa_wichuraiana	Rw5G011000 Rw7G005980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	102, 3032
AasI	GACNNNNNNGTC	1 cut(s)	2629
Acc36I	ACCTGC	1 cut(s)	537
AccB1I	GGYRCC	2 cut(s)	156, 2456
AccB7I	CCANNNNNTGG	1 cut(s)	2597
AccBSI	CCGCTC	1 cut(s)	2815
AccI	GTMKAC	1 cut(s)	697
AccII	CGCG	1 cut(s)	48
AclWI	GGATC	4 cut(s)	1057, 1222, 1276, 3089
AcoI	YGGCCR	1 cut(s)	2146
AcsI	RAATTY	2 cut(s)	729, 1984
AcuI	CTGAAG	5 cut(s)	1815, 2160, 2562, 2966, 3092
AcyI	GRCGYC	2 cut(s)	51, 955
AfaI	GTAC	5 cut(s)	175, 259, 908, 3381, 3463
AfiI	CCNNNNNNNGG	5 cut(s)	47, 367, 1846, 2597, 2925
AflIII	ACRYGT	1 cut(s)	700
AgeI	ACCGGT	2 cut(s)	2018, 2791
AjnI	CCWGG	3 cut(s)	958, 1839, 3230
AjuI	GAANNNNNNNTTGG	2 cut(s)	979, 1011
AloI	GAACNNNNNNTCC	2 cut(s)	2217, 2249
Alw21I	GWGCWC	3 cut(s)	33, 1457, 3181
Alw26I	GTCTC	6 cut(s)	88, 547, 924, 1460, 1551, 3412
Alw44I	GTGCAC	2 cut(s)	1453, 3177
AlwI	GGATC	4 cut(s)	1057, 1222, 1276, 3089
AlwNI	CAGNNNCTG	1 cut(s)	2660
Ama87I	CYCGRG	1 cut(s)	152
AoxI	GGCC	7 cut(s)	38, 510, 1653, 2146, 2185, 2306, 3198
ApaLI	GTGCAC	2 cut(s)	1453, 3177
ApoI	RAATTY	2 cut(s)	729, 1984
AsiGI	ACCGGT	2 cut(s)	2018, 2791
Asp700I	GAANNNNTTC	2 cut(s)	1157, 2232
AspS9I	GGNCC	7 cut(s)	38, 510, 1377, 1653, 2515, 2622, 2852
AsuC2I	CCSGG	2 cut(s)	3202, 3283
AvaI	CYCGRG	1 cut(s)	152
AvaII	GGWCC	4 cut(s)	1377, 2515, 2622, 2852
BaeGI	GKGCMC	6 cut(s)	159, 285, 624, 1457, 2262, 3181
BaeI	ACNNNNGTAYC	4 cut(s)	453, 486, 3363, 3396
BalI	TGGCCA	1 cut(s)	2148
BanI	GGYRCC	2 cut(s)	156, 2456
BauI	CACGAG	1 cut(s)	2864
BbsI	GAAGAC	4 cut(s)	330, 398, 1302, 1982
Bbv12I	GWGCWC	3 cut(s)	33, 1457, 3181
BbvCI	CCTCAGC	1 cut(s)	2058
BccI	CCATC	9 cut(s)	29, 321, 1037, 1485, 1634, 2390, 2429, 2447, 2505
BceAI	ACGGC	2 cut(s)	2200, 3176
BciT130I	CCWGG	3 cut(s)	960, 1841, 3232
BciVI	GTATCC	1 cut(s)	601
BcnI	CCSGG	2 cut(s)	3202, 3283
BcoDI	GTCTC	6 cut(s)	88, 547, 924, 1460, 1551, 3412
BfaI	CTAG	8 cut(s)	347, 836, 1580, 2105, 2574, 2804, 2997, 3269
BfmI	CTRYAG	6 cut(s)	1008, 1884, 2214, 3216, 3293, 3300
BfuAI	ACCTGC	1 cut(s)	537
BfuI	GTATCC	1 cut(s)	601
BglII	AGATCT	3 cut(s)	442, 2130, 2949
BlpI	GCTNAGC	1 cut(s)	1406
Bme1390I	CCNGG	5 cut(s)	960, 1841, 3202, 3232, 3283
Bme18I	GGWCC	4 cut(s)	1377, 2515, 2622, 2852
BmeT110I	CYCGRG	1 cut(s)	152
BmgT120I	GGNCC	7 cut(s)	38, 510, 1377, 1653, 2515, 2622, 2852
BmiI	GGNNCC	6 cut(s)	158, 379, 964, 2458, 2516, 2624
BmrFI	CCNGG	5 cut(s)	960, 1841, 3202, 3232, 3283
BmrI	ACTGGG	1 cut(s)	2875
BmsI	GCATC	7 cut(s)	117, 1099, 1456, 1657, 1886, 2034, 3484
BmuI	ACTGGG	1 cut(s)	2875
BpiI	GAAGAC	4 cut(s)	330, 398, 1302, 1982
BplI	GAGNNNNNCTC	2 cut(s)	2620, 2652
BpmI	CTGGAG	3 cut(s)	108, 1304, 1868
Bpu10I	CCTNAGC	1 cut(s)	2058
Bpu1102I	GCTNAGC	1 cut(s)	1406
BpuEI	CTTGAG	1 cut(s)	3429
BpuMI	CCSGG	2 cut(s)	3202, 3283
Bsa29I	ATCGAT	2 cut(s)	19, 1893
BsaAI	YACGTR	2 cut(s)	257, 701
BsaHI	GRCGYC	2 cut(s)	51, 955
BsaJI	CCNNGG	6 cut(s)	200, 958, 986, 1939, 2555, 3391
BsaWI	WCCGGW	2 cut(s)	2018, 2791
BsaXI	ACNNNNNCTCC	2 cut(s)	2217, 2247
Bsc4I	CCNNNNNNNGG	5 cut(s)	47, 367, 1846, 2597, 2925
Bse118I	RCCGGY	3 cut(s)	403, 2018, 2791
Bse1I	ACTGG	6 cut(s)	216, 730, 1913, 2602, 2758, 2881
Bse3DI	GCAATG	6 cut(s)	1425, 1767, 1951, 2116, 2240, 3207
BseBI	CCWGG	3 cut(s)	960, 1841, 3232
BseCI	ATCGAT	2 cut(s)	19, 1893
BseDI	CCNNGG	6 cut(s)	200, 958, 986, 1939, 2555, 3391
BseLI	CCNNNNNNNGG	5 cut(s)	47, 367, 1846, 2597, 2925
BseMI	GCAATG	6 cut(s)	1425, 1767, 1951, 2116, 2240, 3207
BseMII	CTCAG	6 cut(s)	83, 558, 1047, 1420, 2049, 2948
BseNI	ACTGG	6 cut(s)	216, 730, 1913, 2602, 2758, 2881
BseRI	GAGGAG	1 cut(s)	2426
BseSI	GKGCMC	6 cut(s)	159, 285, 624, 1457, 2262, 3181
BsgI	GTGCAG	3 cut(s)	200, 2174, 2772
Bsh1236I	CGCG	1 cut(s)	48
BshFI	GGCC	7 cut(s)	40, 512, 1655, 2148, 2187, 2308, 3200
BshNI	GGYRCC	2 cut(s)	156, 2456
BshTI	ACCGGT	2 cut(s)	2018, 2791
BshVI	ATCGAT	2 cut(s)	19, 1893
BsiHKAI	GWGCWC	3 cut(s)	33, 1457, 3181
BsiHKCI	CYCGRG	1 cut(s)	152
BsiSI	CCGG	5 cut(s)	404, 2019, 2792, 3201, 3283
BslFI	GGGAC	2 cut(s)	19, 2528
BslI	CCNNNNNNNGG	5 cut(s)	47, 367, 1846, 2597, 2925
BsmAI	GTCTC	6 cut(s)	88, 547, 924, 1460, 1551, 3412
BsmFI	GGGAC	2 cut(s)	19, 2528
BsmI	GAATGC	1 cut(s)	2961
BsnI	GGCC	7 cut(s)	40, 512, 1655, 2148, 2187, 2308, 3200
BsoBI	CYCGRG	1 cut(s)	152
Bsp1286I	GDGCHC	7 cut(s)	33, 159, 285, 624, 1457, 2262, 3181
Bsp1720I	GCTNAGC	1 cut(s)	1406
BspANI	GGCC	7 cut(s)	40, 512, 1655, 2148, 2187, 2308, 3200
BspCNI	CTCAG	6 cut(s)	82, 557, 1046, 1419, 2050, 2949
BspDI	ATCGAT	2 cut(s)	19, 1893
BspFNI	CGCG	1 cut(s)	48
BspHI	TCATGA	3 cut(s)	808, 1462, 2322
BspLI	GGNNCC	6 cut(s)	158, 379, 964, 2458, 2516, 2624
BspMAI	CTGCAG	4 cut(s)	2218, 3220, 3297, 3304
BspMI	ACCTGC	1 cut(s)	537
BspPI	GGATC	4 cut(s)	1057, 1222, 1276, 3089
BspQI	GCTCTTC	1 cut(s)	1938
BspT107I	GGYRCC	2 cut(s)	156, 2456
BsrBI	CCGCTC	1 cut(s)	2815
BsrDI	GCAATG	6 cut(s)	1425, 1767, 1951, 2116, 2240, 3207
BsrFI	RCCGGY	3 cut(s)	403, 2018, 2791
BsrI	ACTGG	6 cut(s)	216, 730, 1913, 2602, 2758, 2881
BssAI	RCCGGY	3 cut(s)	403, 2018, 2791
BssECI	CCNNGG	6 cut(s)	200, 958, 986, 1939, 2555, 3391
BssNAI	GTATAC	1 cut(s)	698
BssNI	GRCGYC	2 cut(s)	51, 955
BssSI	CACGAG	1 cut(s)	2864
BssT1I	CCWWGG	3 cut(s)	986, 1939, 2555
Bst1107I	GTATAC	1 cut(s)	698
Bst2BI	CACGAG	1 cut(s)	2864
Bst2UI	CCWGG	3 cut(s)	960, 1841, 3232
BstACI	GRCGYC	2 cut(s)	51, 955
BstAPI	GCANNNNNTGC	2 cut(s)	1766, 3292
BstBAI	YACGTR	2 cut(s)	257, 701
BstC8I	GCNNGC	9 cut(s)	1758, 2189, 2441, 2603, 2662, 2934, 3117, 3165, 3297
BstDEI	CTNAG	9 cut(s)	69, 425, 544, 1033, 1406, 2058, 2315, 2957, 3522
BstDSI	CCRYGG	1 cut(s)	200
BstFNI	CGCG	1 cut(s)	48
BstMAI	GTCTC	6 cut(s)	88, 547, 924, 1460, 1551, 3412
BstNI	CCWGG	3 cut(s)	960, 1841, 3232
BstNSI	RCATGY	3 cut(s)	1418, 2605, 2654
BstSCI	CCNGG	5 cut(s)	958, 1839, 3200, 3230, 3281
BstSFI	CTRYAG	6 cut(s)	1008, 1884, 2214, 3216, 3293, 3300
BstSLI	GKGCMC	6 cut(s)	159, 285, 624, 1457, 2262, 3181
BstUI	CGCG	1 cut(s)	48
BstV2I	GAAGAC	4 cut(s)	330, 398, 1302, 1982
BstX2I	RGATCY	5 cut(s)	442, 1049, 1227, 2130, 2949
BstXI	CCANNNNNNTGG	1 cut(s)	1387
BstYI	RGATCY	5 cut(s)	442, 1049, 1227, 2130, 2949
BstZ17I	GTATAC	1 cut(s)	698
Bsu15I	ATCGAT	2 cut(s)	19, 1893
BsuI	GTATCC	1 cut(s)	601
BsuRI	GGCC	7 cut(s)	40, 512, 1655, 2148, 2187, 2308, 3200
BsuTUI	ATCGAT	2 cut(s)	19, 1893
BtgI	CCRYGG	1 cut(s)	200
BtsI	GCAGTG	4 cut(s)	867, 2673, 3133, 3486
BveI	ACCTGC	1 cut(s)	537
Cac8I	GCNNGC	9 cut(s)	1758, 2189, 2441, 2603, 2662, 2934, 3117, 3165, 3297
CaiI	CAGNNNCTG	1 cut(s)	2660
CciI	TCATGA	3 cut(s)	808, 1462, 2322
Cfr10I	RCCGGY	3 cut(s)	403, 2018, 2791
Cfr13I	GGNCC	7 cut(s)	38, 510, 1377, 1653, 2515, 2622, 2852
ClaI	ATCGAT	2 cut(s)	19, 1893
CseI	GACGC	5 cut(s)	16, 40, 963, 1259, 2948
Csp6I	GTAC	5 cut(s)	174, 258, 907, 3380, 3462
CspAI	ACCGGT	2 cut(s)	2018, 2791
CviQI	GTAC	5 cut(s)	174, 258, 907, 3380, 3462
DdeI	CTNAG	9 cut(s)	69, 425, 544, 1033, 1406, 2058, 2315, 2957, 3522
DraI	TTTAAA	2 cut(s)	975, 2727
DrdI	GACNNNNNNGTC	1 cut(s)	2629
DseDI	GACNNNNNNGTC	1 cut(s)	2629
EaeI	YGGCCR	1 cut(s)	2146
Eco130I	CCWWGG	3 cut(s)	986, 1939, 2555
Eco32I	GATATC	1 cut(s)	2799
Eco47I	GGWCC	4 cut(s)	1377, 2515, 2622, 2852
Eco57I	CTGAAG	5 cut(s)	1815, 2160, 2562, 2966, 3092
Eco88I	CYCGRG	1 cut(s)	152
EcoO109I	RGGNCCY	1 cut(s)	2515
EcoRI	GAATTC	1 cut(s)	729
EcoRII	CCWGG	3 cut(s)	958, 1839, 3230
EcoRV	GATATC	1 cut(s)	2799
EcoT14I	CCWWGG	3 cut(s)	986, 1939, 2555
EcoT22I	ATGCAT	1 cut(s)	132
ErhI	CCWWGG	3 cut(s)	986, 1939, 2555
FalI	AAGNNNNNCTT	4 cut(s)	312, 344, 464, 496
FaqI	GGGAC	2 cut(s)	19, 2528
FauI	CCCGC	2 cut(s)	1942, 2808
FauNDI	CATATG	1 cut(s)	3376
FblI	GTMKAC	1 cut(s)	697
FspBI	CTAG	8 cut(s)	347, 836, 1580, 2105, 2574, 2804, 2997, 3269
GsuI	CTGGAG	3 cut(s)	108, 1304, 1868
HaeIII	GGCC	7 cut(s)	40, 512, 1655, 2148, 2187, 2308, 3200
HapII	CCGG	5 cut(s)	404, 2019, 2792, 3201, 3283
HgaI	GACGC	5 cut(s)	16, 40, 963, 1259, 2948
Hin1I	GRCGYC	2 cut(s)	51, 955
HincII	GTYRAC	2 cut(s)	276, 2428
HindII	GTYRAC	2 cut(s)	276, 2428
HindIII	AAGCTT	1 cut(s)	1439
HpaII	CCGG	5 cut(s)	404, 2019, 2792, 3201, 3283
Hpy99I	CGWCG	3 cut(s)	29, 957, 2942
HpyCH4IV	ACGT	4 cut(s)	256, 700, 885, 2430
HpyF3I	CTNAG	9 cut(s)	69, 425, 544, 1033, 1406, 2058, 2315, 2957, 3522
HpySE526I	ACGT	4 cut(s)	256, 700, 885, 2430
Hsp92I	GRCGYC	2 cut(s)	51, 955
LguI	GCTCTTC	1 cut(s)	1938
LmnI	GCTCC	8 cut(s)	548, 2119, 2366, 2413, 2530, 2593, 3106, 3112
LweI	GCATC	7 cut(s)	117, 1099, 1456, 1657, 1886, 2034, 3484
MaeI	CTAG	8 cut(s)	347, 836, 1580, 2105, 2574, 2804, 2997, 3269
MaeII	ACGT	4 cut(s)	256, 700, 885, 2430
MaeIII	GTNAC	3 cut(s)	252, 712, 2906
MbiI	CCGCTC	1 cut(s)	2815
MfeI	CAATTG	2 cut(s)	505, 2286
MflI	RGATCY	5 cut(s)	442, 1049, 1227, 2130, 2949
MhlI	GDGCHC	7 cut(s)	33, 159, 285, 624, 1457, 2262, 3181
MlsI	TGGCCA	1 cut(s)	2148
MluNI	TGGCCA	1 cut(s)	2148
MlyI	GAGTC	4 cut(s)	985, 1516, 2328, 3274
MmeI	TCCRAC	5 cut(s)	1105, 2261, 2615, 2865, 3222
Mox20I	TGGCCA	1 cut(s)	2148
Mph1103I	ATGCAT	1 cut(s)	132
MroXI	GAANNNNTTC	2 cut(s)	1157, 2232
MscI	TGGCCA	1 cut(s)	2148
MslI	CAYNNNNRTG	4 cut(s)	495, 813, 1747, 2678
Msp20I	TGGCCA	1 cut(s)	2148
MspA1I	CMGCKG	4 cut(s)	803, 1136, 2645, 2660
MspI	CCGG	5 cut(s)	404, 2019, 2792, 3201, 3283
MspR9I	CCNGG	5 cut(s)	960, 1841, 3202, 3232, 3283
MunI	CAATTG	2 cut(s)	505, 2286
Mva1269I	GAATGC	1 cut(s)	2961
MvaI	CCWGG	3 cut(s)	960, 1841, 3232
MvnI	CGCG	1 cut(s)	48
NciI	CCSGG	2 cut(s)	3202, 3283
NdeI	CATATG	1 cut(s)	3376
NlaIV	GGNNCC	6 cut(s)	158, 379, 964, 2458, 2516, 2624
NmuCI	GTSAC	1 cut(s)	252
NsiI	ATGCAT	1 cut(s)	132
NspI	RCATGY	3 cut(s)	1418, 2605, 2654
PaeI	GCATGC	1 cut(s)	2605
PagI	TCATGA	3 cut(s)	808, 1462, 2322
PciSI	GCTCTTC	1 cut(s)	1938
PcsI	WCGNNNNNNNCGW	2 cut(s)	30, 2190
PctI	GAATGC	1 cut(s)	2961
PdmI	GAANNNNTTC	2 cut(s)	1157, 2232
PfeI	GAWTC	6 cut(s)	21, 766, 790, 1833, 2706, 2993
PflFI	GACNNNGTC	1 cut(s)	426
PflMI	CCANNNNNTGG	1 cut(s)	2597
PinAI	ACCGGT	2 cut(s)	2018, 2791
PleI	GAGTC	4 cut(s)	985, 1516, 2327, 3273
PpsI	GAGTC	4 cut(s)	985, 1516, 2327, 3273
Ppu21I	YACGTR	2 cut(s)	257, 701
PpuMI	RGGWCCY	1 cut(s)	2515
PsiI	TTATAA	2 cut(s)	102, 3032
Psp5II	RGGWCCY	1 cut(s)	2515
Psp6I	CCWGG	3 cut(s)	958, 1839, 3230
PspGI	CCWGG	3 cut(s)	958, 1839, 3230
PspN4I	GGNNCC	6 cut(s)	158, 379, 964, 2458, 2516, 2624
PspPI	GGNCC	7 cut(s)	38, 510, 1377, 1653, 2515, 2622, 2852
PspPPI	RGGWCCY	1 cut(s)	2515
PstI	CTGCAG	4 cut(s)	2218, 3220, 3297, 3304
PstNI	CAGNNNCTG	1 cut(s)	2660
PsuI	RGATCY	5 cut(s)	442, 1049, 1227, 2130, 2949
PsyI	GACNNNGTC	1 cut(s)	426
PvuII	CAGCTG	3 cut(s)	803, 1136, 2660
RsaI	GTAC	5 cut(s)	175, 259, 908, 3381, 3463
RsaNI	GTAC	5 cut(s)	174, 258, 907, 3380, 3462
RseI	CAYNNNNRTG	4 cut(s)	495, 813, 1747, 2678
SapI	GCTCTTC	1 cut(s)	1938
Sau96I	GGNCC	7 cut(s)	38, 510, 1377, 1653, 2515, 2622, 2852
SchI	GAGTC	4 cut(s)	985, 1516, 2328, 3274
ScrFI	CCNGG	5 cut(s)	960, 1841, 3202, 3232, 3283
SduI	GDGCHC	7 cut(s)	33, 159, 285, 624, 1457, 2262, 3181
SfaNI	GCATC	7 cut(s)	117, 1099, 1456, 1657, 1886, 2034, 3484
SfcI	CTRYAG	6 cut(s)	1008, 1884, 2214, 3216, 3293, 3300
SgrAI	CRCCGGYG	1 cut(s)	2791
SinI	GGWCC	4 cut(s)	1377, 2515, 2622, 2852
SmiMI	CAYNNNNRTG	4 cut(s)	495, 813, 1747, 2678
SmlI	CTYRAG	1 cut(s)	3444
SmoI	CTYRAG	1 cut(s)	3444
SphI	GCATGC	1 cut(s)	2605
SspI	AATATT	3 cut(s)	1257, 1541, 1861
SspMI	CTAG	8 cut(s)	347, 836, 1580, 2105, 2574, 2804, 2997, 3269
StyD4I	CCNGG	5 cut(s)	958, 1839, 3200, 3230, 3281
StyI	CCWWGG	3 cut(s)	986, 1939, 2555
TaiI	ACGT	4 cut(s)	259, 703, 888, 2433
TaqI	TCGA	7 cut(s)	19, 24, 952, 1893, 3009, 3066, 3529
TatI	WGTACW	1 cut(s)	3461
TauI	GCSGC	4 cut(s)	51, 2248, 2645, 3238
TfiI	GAWTC	6 cut(s)	21, 766, 790, 1833, 2706, 2993
TseFI	GTSAC	1 cut(s)	252
Tsp45I	GTSAC	1 cut(s)	252
TspGWI	ACGGA	4 cut(s)	161, 1165, 1463, 1688
Tth111I	GACNNNGTC	1 cut(s)	426
Van91I	CCANNNNNTGG	1 cut(s)	2597
VneI	GTGCAC	2 cut(s)	1453, 3177
VpaK11BI	GGWCC	4 cut(s)	1377, 2515, 2622, 2852
XapI	RAATTY	2 cut(s)	729, 1984
XbaI	TCTAGA	1 cut(s)	346
XceI	RCATGY	3 cut(s)	1418, 2605, 2654
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1837, 3238
XmiI	GTMKAC	1 cut(s)	697
XmnI	GAANNNNTTC	2 cut(s)	1157, 2232
XspI	CTAG	8 cut(s)	347, 836, 1580, 2105, 2574, 2804, 2997, 3269
Zsp2I	ATGCAT	1 cut(s)	132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.