Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000685

Physical Location & Seq

Genomic Coordinates Reverse (-)

32487498 .. 32491418

Gene Structure 3921 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1228200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGCAACTGTTACACGTGTCGACAAGAGAACCTATTCCGCCATTAATCCCACCTCCATTTTCACCTCCTGCAGTTTCCATGATTCAGTGGTTTTGGTCAGCTATTTGGTGCTTCTGCTTTCTTCATCAACCATCACTCCATCTTTTGGGGTTTCTGGTGTTGGAGAAGAGAGCGGTTCGTATCCGAAAATTCTGAGGGATGAGGCTGTGGCTAGACTTCACCAGCTTGGACGGGTAAGTGATGGTGATGGATATCTTGAGAGGACATTCATGAGCCCGGCTGCTCTGAGGGCAGGAAATCTTATCCGGGAATGGATGGAAGACGCGGGTTTGAGAACATGGGTTGACTGCTTGGGGAATGTACACGGTCGAGTTGAGGGAAGGAATGCTAGTGCGGAAGCTCTGTTAATGGGTTCTCATTTGGATACTGTTGTTGATGGTGGGAAATTTGATGGAGCATTAGGCATCATTTCTGTGTTATCTGCATTGAAGGTTTTGAAGGTCAATGGAAAGTTGGGAGAACTAAGCCGGCCAGTTGAGGTAATAGCATTTAGTGATGAAGAGGGAGTAAGGTATCAGTCTACATTCTTAGGCAGTGCTGCTGTAGCAGGAATTTTACCCGTTTCAGCACTCAAAATATCCGATAAAAGTGATGTAACAATTGAAGATGCTCTTAAGAAGAACTCCATAGAAATTACAGAGGAGCACTTGTTGCAGCTTAAATATGACCCAAAGTCGGTCTGGGGTTATGTTGAGATTCATATTGAACAAGGACCTGTACTAGAATGGGTTGGCTTTCCTCTAGCTGTGGTTAAAGGCATAGCTGGACAGACACGAATAAAGGTTACGATGAGAGGTTCCCAGGGGCATGCTGGAACAGTTCCAATGTCTATGCGTCGTGACCCTATGGCTGCTGCTGCTGAAGCAATTTATTCATTAGAAAGCTTATGTAAAAGCCCTCAAGATTTTTTGTCTTTTGATGGTCAATGCAAAAGTTTCTCGATGGAGTCGCTTTCAGCTTCGCTTGTTTGTACTGTTGGAGAGATATCAACTTGGCCGAGTGCAAGCAACGTCATTCCTGGCCAGGTAACATTCACCGTAGATTTACGTACGATAGATGACATGGGACGTGAAGCTGTTGTGTATGAGTTTTCTAACCAAGTGTATCAAATTTGTGATAGACGCTCTGTTTCTTGTACAATTGATCGTAAGCATGATGCAGGTGCAGCCATTTGTGATTCTGAGTTGAGTTCAAAGCTGAAATCTGCAGCATATATCGGGCTCAAGAGAATGACAGGTGCAGCCATTGAGGATGAAATTCCCGTGTTAATGAGTGGAGCAGGACACGATGCAATGGCTCTATCTCATTTAACCAAGGTGGGAATGCTTTTCGTGCGCTGTCGAGGAGGCGTAAGTCACTCCCCTGAAGAGCATGTATTGGACGACGATGTTTGGGCATCTGGTTTGGCAATTTTGGCATTCATAGAGACTCAACTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000255 GO:0000256 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009987 GO:0010135 GO:0010136 GO:0012505 GO:0016787 GO:0016810 GO:0016813 GO:0017144 GO:0019439 GO:0034641 GO:0042737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043605 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046113 GO:0046483 GO:0046700 GO:0047652 GO:0055086 GO:0071704 GO:0072521 GO:0072523 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00230 ko01120 map00230 map01120

Pfam Domains

Protein Families

M20_dimer Peptidase_M20 Peptidase_M28

Protein Analysis

500

Amino Acids

53.72

Weight (kDa)

5.32

Isoelectric Point (pI)

37.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M28	PF04389	120 - 203	2.4e-08	Peptidase family M28
Peptidase_M20	PF01546	137 - 486	2.5e-13	Peptidase family M20/M25/M40
M20_dimer	PF07687	276 - 388	1.5e-07	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000685)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20070 AT4G20070
fragaria_vesca	FvH4_3g12850 FvH4_3g12850 FvH4_3g12850
malus_domestica	MD10G1228200.v1.1
prunus_persica	Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1
pyrus_communis	pycom10g19170
rosa_chinensis	RchiOBHm_Chr1g0315031 RchiOBHm_Chr1g0315061 RchiOBHm_Chr1g0315091 RchiOBHm_Chr1g0315141 RchiOBHm_Chr1g0315151 RchiOBHm_Chr1g0315201 RchiOBHm_Chr1g0315231 RchiOBHm_Chr1g0315241 RchiOBHm_Chr1g0315311 RchiOBHm_Chr1g0315381 RchiOBHm_Chr1g0315441 RchiOBHm_Chr1g0315471 RchiOBHm_Chr1g0315531 RchiOBHm_Chr1g0315541 RchiOBHm_Chr1g0315581 RchiOBHm_Chr1g0315591 RchiOBHm_Chr1g0315611 RchiOBHm_Chr5g0020791 RchiOBHm_Chr5g0020891 RchiOBHm_Chr5g0021441 RchiOBHm_Chr5g0021511 RchiOBHm_Chr5g0021521 RchiOBHm_Chr5g0029451 RchiOBHm_Chr5g0029471
rosa_laevigata	RLG00000030744 RLG00000030748 RLG00000032563 RLG00000032611
rosa_multiflora	Rmu_sc0000576.1_g000021 Rmu_sc0002052.1_g000003 Rmu_sc0004180.1_g000017 Rmu_sc0004180.1_g000030 Rmu_sc0004180.1_g000035 Rmu_sc0023070.1_g000002 Rmu_ssc0000244.1_g000002
rosa_roxburghii	Rroxscaffold_1G00057130 Rroxscaffold_1G00057510 Rroxscaffold_4G00331580
rosa_rugosa	Rorug01G0004300 Rorug05G0063000 Rorug05G0067300
rosa_samantha	Rh1AG015000 Rh1AG015600 Rh1BG007400 Rh1BG007800 Rh1CG013200 Rh5AG152900 Rh5BG152700 Rh5BG155800 Rh5CG164200 Rh5CG164700 Rh5CG170000 Rh5CG227100
rosa_wichuraiana	Rw1G000740 Rw1G000780 Rw1G000810 Rw1G000840 Rw1G000870 Rw1G000900 Rw5G013560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1211
Acc36I	ACCTGC	1 cut(s)	1211
AccB7I	CCANNNNNTGG	1 cut(s)	146
AccBSI	CCGCTC	1 cut(s)	174
AccI	GTMKAC	2 cut(s)	21, 581
AccII	CGCG	1 cut(s)	326
AciI	CCGC	4 cut(s)	39, 174, 326, 395
AcoI	YGGCCR	3 cut(s)	530, 1055, 1081
AcsI	RAATTY	5 cut(s)	189, 446, 612, 1170, 1317
AcuI	CTGAAG	2 cut(s)	942, 1446
AcvI	CACGTG	1 cut(s)	17
AfaI	GTAC	5 cut(s)	363, 780, 1033, 1111, 1198
AfiI	CCNNNNNNNGG	2 cut(s)	146, 736
AflII	CTTAAG	1 cut(s)	674
AflIII	ACRYGT	2 cut(s)	14, 16
AgsI	TTSAA	5 cut(s)	490, 499, 665, 767, 1254
AhdI	GACNNNNNGTC	1 cut(s)	733
AjiI	CACGTC	1 cut(s)	1130
AjnI	CCWGG	3 cut(s)	861, 1078, 1083
Alw21I	GWGCWC	1 cut(s)	708
Alw26I	GTCTC	1 cut(s)	1481
AoxI	GGCC	3 cut(s)	530, 1055, 1081
ApeKI	GCWGC	9 cut(s)	281, 599, 715, 911, 914, 917, 1226, 1268, 1301
ApoI	RAATTY	5 cut(s)	189, 446, 612, 1170, 1317
AseI	ATTAAT	1 cut(s)	45
Asp700I	GAANNNNTTC	1 cut(s)	34
AspLEI	GCGC	1 cut(s)	1398
AspS9I	GGNCC	1 cut(s)	773
AsuC2I	CCSGG	2 cut(s)	278, 308
AsuHPI	GGTGA	4 cut(s)	55, 212, 257, 1087
AvaII	GGWCC	1 cut(s)	773
BaeI	ACNNNNGTAYC	2 cut(s)	417, 450
BalI	TGGCCA	1 cut(s)	1083
BanII	GRGCYC	2 cut(s)	278, 1284
BbrPI	CACGTG	1 cut(s)	17
BbsI	GAAGAC	1 cut(s)	327
Bbv12I	GWGCWC	1 cut(s)	708
BbvI	GCAGC	9 cut(s)	268, 586, 727, 898, 901, 904, 1238, 1280, 1313
BccI	CCATC	9 cut(s)	140, 148, 236, 242, 310, 431, 446, 974, 997
BciT130I	CCWGG	3 cut(s)	863, 1080, 1085
BciVI	GTATCC	2 cut(s)	192, 418
BcnI	CCSGG	2 cut(s)	278, 308
BcoDI	GTCTC	1 cut(s)	1481
BfaI	CTAG	4 cut(s)	213, 390, 782, 803
BfmI	CTRYAG	3 cut(s)	70, 603, 1266
BfrI	CTTAAG	1 cut(s)	674
BfuAI	ACCTGC	1 cut(s)	1211
BfuI	GTATCC	2 cut(s)	192, 418
BisI	GCNGC	9 cut(s)	282, 600, 716, 912, 915, 918, 1227, 1269, 1302
BlsI	GCNGC	9 cut(s)	283, 601, 717, 913, 916, 919, 1228, 1270, 1303
Bme1390I	CCNGG	5 cut(s)	278, 308, 863, 1080, 1085
Bme18I	GGWCC	1 cut(s)	773
BmeRI	GACNNNNNGTC	1 cut(s)	733
BmgBI	CACGTC	1 cut(s)	1130
BmgT120I	GGNCC	1 cut(s)	773
BmiI	GGNNCC	1 cut(s)	859
BmrFI	CCNGG	5 cut(s)	278, 308, 863, 1080, 1085
BmsI	GCATC	5 cut(s)	474, 658, 1207, 1339, 1466
BpiI	GAAGAC	1 cut(s)	327
BpuEI	CTTGAG	3 cut(s)	278, 945, 1268
BpuMI	CCSGG	2 cut(s)	278, 308
BsaAI	YACGTR	2 cut(s)	17, 1109
BsaBI	GATNNNNATC	1 cut(s)	252
BsaJI	CCNNGG	3 cut(s)	861, 862, 1374
BsaXI	ACNNNNNCTCC	4 cut(s)	121, 151, 695, 725
Bsc4I	CCNNNNNNNGG	2 cut(s)	146, 736
Bse118I	RCCGGY	1 cut(s)	528
Bse1I	ACTGG	1 cut(s)	533
Bse3DI	GCAATG	1 cut(s)	1359
Bse8I	GATNNNNATC	1 cut(s)	252
BseBI	CCWGG	3 cut(s)	863, 1080, 1085
BseDI	CCNNGG	3 cut(s)	861, 862, 1374
BseGI	GGATG	3 cut(s)	205, 321, 1318
BseJI	GATNNNNATC	1 cut(s)	252
BseLI	CCNNNNNNNGG	2 cut(s)	146, 736
BseMI	GCAATG	1 cut(s)	1359
BseMII	CTCAG	3 cut(s)	185, 278, 1233
BseNI	ACTGG	1 cut(s)	533
BseRI	GAGGAG	2 cut(s)	716, 1419
BseXI	GCAGC	9 cut(s)	268, 586, 727, 898, 901, 904, 1238, 1280, 1313
BsgI	GTGCAG	2 cut(s)	1245, 1320
Bsh1236I	CGCG	1 cut(s)	326
Bsh1285I	CGRYCG	1 cut(s)	370
BshFI	GGCC	3 cut(s)	532, 1057, 1083
BsiEI	CGRYCG	1 cut(s)	370
BsiHKAI	GWGCWC	1 cut(s)	708
BsiSI	CCGG	3 cut(s)	278, 307, 529
BsiWI	CGTACG	1 cut(s)	1109
BslFI	GGGAC	1 cut(s)	1140
BslI	CCNNNNNNNGG	2 cut(s)	146, 736
BsmAI	GTCTC	1 cut(s)	1481
BsmFI	GGGAC	1 cut(s)	1140
BsmI	GAATGC	3 cut(s)	391, 1389, 1478
BsnI	GGCC	3 cut(s)	532, 1057, 1083
Bsp1286I	GDGCHC	3 cut(s)	278, 708, 1284
Bsp1407I	TGTACA	2 cut(s)	361, 1196
Bsp143I	GATC	1 cut(s)	1204
BspACI	CCGC	4 cut(s)	39, 174, 326, 395
BspANI	GGCC	3 cut(s)	532, 1057, 1083
BspCNI	CTCAG	3 cut(s)	186, 279, 1234
BspFNI	CGCG	1 cut(s)	326
BspHI	TCATGA	1 cut(s)	270
BspLI	GGNNCC	1 cut(s)	859
BspMAI	CTGCAG	2 cut(s)	74, 1270
BspMI	ACCTGC	1 cut(s)	1211
BspQI	GCTCTTC	1 cut(s)	1422
BspTI	CTTAAG	1 cut(s)	674
BsrBI	CCGCTC	1 cut(s)	174
BsrDI	GCAATG	1 cut(s)	1359
BsrFI	RCCGGY	1 cut(s)	528
BsrGI	TGTACA	2 cut(s)	361, 1196
BsrI	ACTGG	1 cut(s)	533
BssAI	RCCGGY	1 cut(s)	528
BssECI	CCNNGG	3 cut(s)	861, 862, 1374
BssMI	GATC	1 cut(s)	1204
BssT1I	CCWWGG	1 cut(s)	1374
Bst2UI	CCWGG	3 cut(s)	863, 1080, 1085
Bst4CI	ACNGT	7 cut(s)	10, 368, 430, 880, 1036, 1099, 1497
Bst6I	CTCTTC	3 cut(s)	162, 555, 1422
BstAFI	CTTAAG	1 cut(s)	674
BstAPI	GCANNNNNTGC	1 cut(s)	712
BstAUI	TGTACA	2 cut(s)	361, 1196
BstBAI	YACGTR	2 cut(s)	17, 1109
BstC8I	GCNNGC	3 cut(s)	530, 870, 1066
BstDEI	CTNAG	5 cut(s)	194, 287, 524, 589, 1242
BstF5I	GGATG	3 cut(s)	205, 321, 1318
BstFNI	CGCG	1 cut(s)	326
BstHHI	GCGC	1 cut(s)	1398
BstKTI	GATC	1 cut(s)	1207
BstMAI	GTCTC	1 cut(s)	1481
BstMBI	GATC	1 cut(s)	1204
BstMCI	CGRYCG	1 cut(s)	370
BstMWI	GCNNNNNNNGC	8 cut(s)	290, 605, 712, 917, 923, 1226, 1393, 1475
BstNI	CCWGG	3 cut(s)	863, 1080, 1085
BstNSI	RCATGY	2 cut(s)	872, 1436
BstSCI	CCNGG	5 cut(s)	276, 306, 861, 1078, 1083
BstSFI	CTRYAG	3 cut(s)	70, 603, 1266
BstSNI	TACGTA	1 cut(s)	1109
BstUI	CGCG	1 cut(s)	326
BstV1I	GCAGC	9 cut(s)	268, 586, 727, 898, 901, 904, 1238, 1280, 1313
BstV2I	GAAGAC	1 cut(s)	327
BsuI	GTATCC	2 cut(s)	192, 418
BsuRI	GGCC	3 cut(s)	532, 1057, 1083
BtrI	CACGTC	1 cut(s)	1130
BtsCI	GGATG	3 cut(s)	205, 321, 1318
BtsI	GCAGTG	1 cut(s)	601
BtsIMutI	CAGTG	2 cut(s)	93, 601
BveI	ACCTGC	1 cut(s)	1211
Cac8I	GCNNGC	3 cut(s)	530, 870, 1066
CciI	TCATGA	1 cut(s)	270
CfoI	GCGC	1 cut(s)	1398
Cfr10I	RCCGGY	1 cut(s)	528
Cfr13I	GGNCC	1 cut(s)	773
CseI	GACGC	3 cut(s)	332, 884, 1191
Csp6I	GTAC	5 cut(s)	362, 779, 1032, 1110, 1197
CviAII	CATG	7 cut(s)	80, 271, 339, 869, 1123, 1214, 1433
CviQI	GTAC	5 cut(s)	362, 779, 1032, 1110, 1197
DdeI	CTNAG	5 cut(s)	194, 287, 524, 589, 1242
DpnI	GATC	1 cut(s)	1206
DpnII	GATC	1 cut(s)	1204
DriI	GACNNNNNGTC	1 cut(s)	733
EaeI	YGGCCR	3 cut(s)	530, 1055, 1081
Eam1104I	CTCTTC	3 cut(s)	162, 555, 1422
Eam1105I	GACNNNNNGTC	1 cut(s)	733
EarI	CTCTTC	3 cut(s)	162, 555, 1422
EciI	GGCGGA	1 cut(s)	28
Eco105I	TACGTA	1 cut(s)	1109
Eco130I	CCWWGG	1 cut(s)	1374
Eco24I	GRGCYC	2 cut(s)	278, 1284
Eco32I	GATATC	2 cut(s)	254, 1047
Eco47I	GGWCC	1 cut(s)	773
Eco57I	CTGAAG	2 cut(s)	942, 1446
Eco72I	CACGTG	1 cut(s)	17
EcoO109I	RGGNCCY	1 cut(s)	773
EcoRII	CCWGG	3 cut(s)	861, 1078, 1083
EcoRV	GATATC	2 cut(s)	254, 1047
EcoT14I	CCWWGG	1 cut(s)	1374
EcoT38I	GRGCYC	2 cut(s)	278, 1284
ErhI	CCWWGG	1 cut(s)	1374
FaeI	CATG	7 cut(s)	83, 274, 342, 872, 1126, 1217, 1436
FalI	AAGNNNNNCTT	2 cut(s)	657, 689
FaqI	GGGAC	1 cut(s)	1140
FatI	CATG	7 cut(s)	79, 270, 338, 868, 1122, 1213, 1432
FauI	CCCGC	1 cut(s)	319
FblI	GTMKAC	2 cut(s)	21, 581
Fnu4HI	GCNGC	9 cut(s)	282, 600, 716, 912, 915, 918, 1227, 1269, 1302
FokI	GGATG	3 cut(s)	212, 328, 1325
FriOI	GRGCYC	2 cut(s)	278, 1284
Fsp4HI	GCNGC	9 cut(s)	282, 600, 716, 912, 915, 918, 1227, 1269, 1302
FspBI	CTAG	4 cut(s)	213, 390, 782, 803
GlaI	GCGC	1 cut(s)	1397
GluI	GCNGC	9 cut(s)	282, 600, 716, 912, 915, 918, 1227, 1269, 1302
HaeIII	GGCC	3 cut(s)	532, 1057, 1083
HapII	CCGG	3 cut(s)	278, 307, 529
HgaI	GACGC	3 cut(s)	332, 884, 1191
HhaI	GCGC	1 cut(s)	1398
Hin1II	CATG	7 cut(s)	83, 274, 342, 872, 1126, 1217, 1436
Hin6I	GCGC	1 cut(s)	1396
HinP1I	GCGC	1 cut(s)	1396
HincII	GTYRAC	2 cut(s)	22, 346
HindII	GTYRAC	2 cut(s)	22, 346
HindIII	AAGCTT	1 cut(s)	943
HinfI	GANTC	5 cut(s)	83, 757, 1007, 1238, 1489
HpaII	CCGG	3 cut(s)	278, 307, 529
HphI	GGTGA	4 cut(s)	55, 212, 257, 1087
Hpy166II	GTNNAC	4 cut(s)	22, 346, 364, 582
Hpy188I	TCNGA	5 cut(s)	186, 195, 288, 643, 1243
Hpy188III	TCNNGA	6 cut(s)	257, 271, 899, 962, 1000, 1285
Hpy8I	GTNNAC	4 cut(s)	22, 346, 364, 582
Hpy99I	CGWCG	2 cut(s)	900, 1448
HpyAV	CCTTC	3 cut(s)	375, 484, 493
HpyCH4III	ACNGT	7 cut(s)	10, 368, 430, 880, 1036, 1099, 1497
HpyCH4IV	ACGT	4 cut(s)	16, 1071, 1108, 1129
HpyF10VI	GCNNNNNNNGC	8 cut(s)	290, 605, 712, 917, 923, 1226, 1393, 1475
HpyF3I	CTNAG	5 cut(s)	194, 287, 524, 589, 1242
HpySE526I	ACGT	4 cut(s)	16, 1071, 1108, 1129
Hsp92II	CATG	7 cut(s)	83, 274, 342, 872, 1126, 1217, 1436
HspAI	GCGC	1 cut(s)	1396
KroI	GCCGGC	1 cut(s)	528
KroNI	GCCGGC	1 cut(s)	530
Kzo9I	GATC	1 cut(s)	1204
LguI	GCTCTTC	1 cut(s)	1422
LmnI	GCTCC	3 cut(s)	455, 703, 1337
Lsp1109I	GCAGC	9 cut(s)	268, 586, 727, 898, 901, 904, 1238, 1280, 1313
LweI	GCATC	5 cut(s)	474, 658, 1207, 1339, 1466
MaeI	CTAG	4 cut(s)	213, 390, 782, 803
MaeII	ACGT	4 cut(s)	16, 1071, 1108, 1129
MaeIII	GTNAC	6 cut(s)	10, 655, 844, 899, 1087, 1415
MalI	GATC	1 cut(s)	1206
MbiI	CCGCTC	1 cut(s)	174
MboI	GATC	1 cut(s)	1204
MboII	GAAGA	7 cut(s)	114, 179, 332, 572, 677, 691, 1439
MfeI	CAATTG	2 cut(s)	660, 1200
MhlI	GDGCHC	3 cut(s)	278, 708, 1284
MlsI	TGGCCA	1 cut(s)	1083
MluNI	TGGCCA	1 cut(s)	1083
MlyI	GAGTC	2 cut(s)	1016, 1483
MmeI	TCCRAC	2 cut(s)	142, 1018
Mox20I	TGGCCA	1 cut(s)	1083
MroNI	GCCGGC	1 cut(s)	528
MroXI	GAANNNNTTC	1 cut(s)	34
MscI	TGGCCA	1 cut(s)	1083
MseI	TTAA	7 cut(s)	45, 407, 675, 720, 813, 1328, 1370
MslI	CAYNNNNRTG	1 cut(s)	473
Msp20I	TGGCCA	1 cut(s)	1083
MspCI	CTTAAG	1 cut(s)	674
MspI	CCGG	3 cut(s)	278, 307, 529
MspR9I	CCNGG	5 cut(s)	278, 308, 863, 1080, 1085
MunI	CAATTG	2 cut(s)	660, 1200
Mva1269I	GAATGC	3 cut(s)	391, 1389, 1478
MvaI	CCWGG	3 cut(s)	863, 1080, 1085
MvnI	CGCG	1 cut(s)	326
MwoI	GCNNNNNNNGC	8 cut(s)	290, 605, 712, 917, 923, 1226, 1393, 1475
NaeI	GCCGGC	1 cut(s)	530
NciI	CCSGG	2 cut(s)	278, 308
NdeII	GATC	1 cut(s)	1204
NgoMIV	GCCGGC	1 cut(s)	528
NlaIII	CATG	7 cut(s)	83, 274, 342, 872, 1126, 1217, 1436
NlaIV	GGNNCC	1 cut(s)	859
NmeAIII	GCCGAG	1 cut(s)	1083
NmuCI	GTSAC	2 cut(s)	899, 1415
NspI	RCATGY	2 cut(s)	872, 1436
PaeI	GCATGC	1 cut(s)	872
PagI	TCATGA	1 cut(s)	270
PaqCI	CACCTGC	1 cut(s)	1211
PasI	CCCWGGG	1 cut(s)	862
PciSI	GCTCTTC	1 cut(s)	1422
PctI	GAATGC	3 cut(s)	391, 1389, 1478
PdiI	GCCGGC	1 cut(s)	530
PdmI	GAANNNNTTC	1 cut(s)	34
PfeI	GAWTC	3 cut(s)	83, 757, 1238
Pfl23II	CGTACG	1 cut(s)	1109
PflMI	CCANNNNNTGG	1 cut(s)	146
PfoI	TCCNGGA	1 cut(s)	306
PkrI	GCNGC	9 cut(s)	283, 601, 717, 913, 916, 919, 1228, 1270, 1303
PleI	GAGTC	2 cut(s)	1015, 1483
PmaCI	CACGTG	1 cut(s)	17
PmlI	CACGTG	1 cut(s)	17
PpsI	GAGTC	2 cut(s)	1015, 1483
Ppu21I	YACGTR	2 cut(s)	17, 1109
PpuMI	RGGWCCY	1 cut(s)	773
PshBI	ATTAAT	1 cut(s)	45
Psp5II	RGGWCCY	1 cut(s)	773
Psp6I	CCWGG	3 cut(s)	861, 1078, 1083
PspCI	CACGTG	1 cut(s)	17
PspGI	CCWGG	3 cut(s)	861, 1078, 1083
PspLI	CGTACG	1 cut(s)	1109
PspN4I	GGNNCC	1 cut(s)	859
PspPI	GGNCC	1 cut(s)	773
PspPPI	RGGWCCY	1 cut(s)	773
PstI	CTGCAG	2 cut(s)	74, 1270
RsaI	GTAC	5 cut(s)	363, 780, 1033, 1111, 1198
RsaNI	GTAC	5 cut(s)	362, 779, 1032, 1110, 1197
RseI	CAYNNNNRTG	1 cut(s)	473
SalI	GTCGAC	1 cut(s)	20
SapI	GCTCTTC	1 cut(s)	1422
SaqAI	TTAA	7 cut(s)	45, 407, 675, 720, 813, 1328, 1370
SatI	GCNGC	9 cut(s)	282, 600, 716, 912, 915, 918, 1227, 1269, 1302
Sau3AI	GATC	1 cut(s)	1204
Sau96I	GGNCC	1 cut(s)	773
SchI	GAGTC	2 cut(s)	1016, 1483
ScrFI	CCNGG	5 cut(s)	278, 308, 863, 1080, 1085
SduI	GDGCHC	3 cut(s)	278, 708, 1284
SfaNI	GCATC	5 cut(s)	474, 658, 1207, 1339, 1466
SfcI	CTRYAG	3 cut(s)	70, 603, 1266
SinI	GGWCC	1 cut(s)	773
SmiMI	CAYNNNNRTG	1 cut(s)	473
SmlI	CTYRAG	4 cut(s)	257, 674, 960, 1283
SmoI	CTYRAG	4 cut(s)	257, 674, 960, 1283
SnaBI	TACGTA	1 cut(s)	1109
SphI	GCATGC	1 cut(s)	872
SsiI	CCGC	4 cut(s)	39, 174, 326, 395
SspMI	CTAG	4 cut(s)	213, 390, 782, 803
StyD4I	CCNGG	5 cut(s)	276, 306, 861, 1078, 1083
StyI	CCWWGG	1 cut(s)	1374
TaaI	ACNGT	7 cut(s)	10, 368, 430, 880, 1036, 1099, 1497
TaiI	ACGT	4 cut(s)	19, 1074, 1111, 1132
TaqI	TCGA	4 cut(s)	21, 370, 1001, 1402
TaqII	GACCGA	1 cut(s)	727
TatI	WGTACW	4 cut(s)	361, 778, 1031, 1196
TfiI	GAWTC	3 cut(s)	83, 757, 1238
Tru1I	TTAA	7 cut(s)	45, 407, 675, 720, 813, 1328, 1370
Tru9I	TTAA	7 cut(s)	45, 407, 675, 720, 813, 1328, 1370
TscAI	CASTG	2 cut(s)	93, 601
TseFI	GTSAC	2 cut(s)	899, 1415
TseI	GCWGC	9 cut(s)	281, 599, 715, 911, 914, 917, 1226, 1268, 1301
Tsp45I	GTSAC	2 cut(s)	899, 1415
TspDTI	ATGAA	7 cut(s)	114, 259, 573, 749, 924, 1329, 1471
TspRI	CASTG	2 cut(s)	93, 601
Van91I	CCANNNNNTGG	1 cut(s)	146
Vha464I	CTTAAG	1 cut(s)	674
VpaK11BI	GGWCC	1 cut(s)	773
VspI	ATTAAT	1 cut(s)	45
XapI	RAATTY	5 cut(s)	189, 446, 612, 1170, 1317
XceI	RCATGY	2 cut(s)	872, 1436
XcmI	CCANNNNNNNNNTGG	2 cut(s)	738, 869
XmiI	GTMKAC	2 cut(s)	21, 581
XmnI	GAANNNNTTC	1 cut(s)	34
XspI	CTAG	4 cut(s)	213, 390, 782, 803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.