Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0000685

Physical Location & Seq

Genomic Coordinates Forward (+)

1116488 .. 1119667

Gene Structure 3180 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G000780.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGACAGTTGTTACACGTGTCTCCCAGAGAACCCATTCCGCCATTAGTCCCACCTCCGTTTTGGCTCTGGCTTGCCATTTTTTGCTTCTGGTCTCTTCAGCCATCACTCCCTGTTTTGGATTTTCCGATGAGAGAAACAGAGGAGGGGATTTGTATCAGGAAATATTGAGAGATGAAGCTGTGGCAAGACTTCATCAGCTTGGAAAGGTAAGTGATGGTGATGGTTATCTTGAGAGGATATTCATGAGCCCGGCAGCTGTGAGGGCTAGAAATCTTATCCGCGAATGGATGGAGGATGCTGGTTTGTGGGTTCATTGCTTGGGAAATGTACATGGTCGAGTTGAGGGCACAAATGCTAGTGCTGAGGTTCTAATACTTGGTTCTCATTTGGTACTGATACTGTTGTTGATGCCGGGATATTTGATGGGTCTTTACTCTTTAGGCATCATTTCTGTGTTATCTGCATTGAAGGTTTTGAGTATCGATGGAAAATTGGGAGAACTAAAGCGGCCGGTTGAGGTAATAGCATTTAGTGATGAAGAAGGAGTAAGGTTTCAGTCTACATTCATAGGAAGTGCTGCTATAGCTGGAATTTTACCAGTTTCAGCACTGCAAATAACTGATAAAAGTGGTGTGACAATTCAAGATGCTCTTAAGAAAAACTCCATTGAAGTTACAGAGGAGCACTTGCAGCAGCTCAGATATGATCCAAAGTCAGTTTGGGGTTATGTTGAGATTCACATTGAACAAGGGCCTGTACTGGAATGGGTTGGTTTTCCTCTAGGAGATGTTAAAGGCATAGCTGGACAGACAAGACTAAAGGTTACAATGAGAGGTTCCCAGGGGCATGCTGGAACAGTTCCAATGTCCATGCGTCATGACCCTATGGCTGCTGCTGCGGAAGCCATTATATTGTTAGAAAGCCTATGTAAGCATCCTCAAGATTTTCTGTCTTTTGATGGTCAATGCAAAAGTTACTCATTAGAATCACTTTCAACTTCCCTTGTTTGTACCGTTTGTGAGATATCGACTTGGCCAAGTGCAAGTAATGTTATTCCAGGACAGGTAACATTCACAGTAGATTTACGTGCAATTGATGACATGGGACGTGAAGCTGTTGTATGTGAATTATCTAACCGATTGTATCAAATATGTGATAGGCGTTCAGTTTCGTGCACAATTGATCGTAAGCATGATGCAAATGCAGTCATTTGCGATTCTGAGCTGACTTCGAAGCTGAAGTCAGCAGCTTATCTTGGGCTCAAGAGAATGACAGGTTCTGTTCAGGATGAAGTACCTGTATTAATGAGTGGAGCAGTATTAATGAGTGGAGCAGGACATGATGCAATGGCTCTGTCTCATTTAACTAAGGTGGGTATGCTTTTTGTCCGCTGTCGAGGAGGCATAAGTCACTTCCCTGAAGAACATATATTGGATGATATGATGTTTGGGCATCTGGTTTGGCAATCATGGCATTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000255 GO:0000256 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009987 GO:0010135 GO:0010136 GO:0012505 GO:0016787 GO:0016810 GO:0016813 GO:0017144 GO:0019439 GO:0034641 GO:0042737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043605 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046113 GO:0046483 GO:0046700 GO:0047652 GO:0055086 GO:0071704 GO:0072521 GO:0072523 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00230 ko01120 map00230 map01120

Pfam Domains

Protein Families

M20_dimer Peptidase_M20 Peptidase_M28

Protein Analysis

493

Amino Acids

53.77

Weight (kDa)

5.91

Isoelectric Point (pI)

41.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	151 - 480	3.5e-08	Peptidase family M20/M25/M40
M20_dimer	PF07687	269 - 381	7.5e-07	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000685)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20070 AT4G20070
fragaria_vesca	FvH4_3g12850 FvH4_3g12850 FvH4_3g12850
malus_domestica	MD10G1228200.v1.1
prunus_persica	Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1
pyrus_communis	pycom10g19170
rosa_chinensis	RchiOBHm_Chr1g0315031 RchiOBHm_Chr1g0315061 RchiOBHm_Chr1g0315091 RchiOBHm_Chr1g0315141 RchiOBHm_Chr1g0315151 RchiOBHm_Chr1g0315201 RchiOBHm_Chr1g0315231 RchiOBHm_Chr1g0315241 RchiOBHm_Chr1g0315311 RchiOBHm_Chr1g0315381 RchiOBHm_Chr1g0315441 RchiOBHm_Chr1g0315471 RchiOBHm_Chr1g0315531 RchiOBHm_Chr1g0315541 RchiOBHm_Chr1g0315581 RchiOBHm_Chr1g0315591 RchiOBHm_Chr1g0315611 RchiOBHm_Chr5g0020791 RchiOBHm_Chr5g0020891 RchiOBHm_Chr5g0021441 RchiOBHm_Chr5g0021511 RchiOBHm_Chr5g0021521 RchiOBHm_Chr5g0029451 RchiOBHm_Chr5g0029471
rosa_laevigata	RLG00000030744 RLG00000030748 RLG00000032563 RLG00000032611
rosa_multiflora	Rmu_sc0000576.1_g000021 Rmu_sc0002052.1_g000003 Rmu_sc0004180.1_g000017 Rmu_sc0004180.1_g000030 Rmu_sc0004180.1_g000035 Rmu_sc0023070.1_g000002 Rmu_ssc0000244.1_g000002
rosa_roxburghii	Rroxscaffold_1G00057130 Rroxscaffold_1G00057510 Rroxscaffold_4G00331580
rosa_rugosa	Rorug01G0004300 Rorug05G0063000 Rorug05G0067300
rosa_samantha	Rh1AG015000 Rh1AG015600 Rh1BG007400 Rh1BG007800 Rh1CG013200 Rh5AG152900 Rh5BG152700 Rh5BG155800 Rh5CG164200 Rh5CG164700 Rh5CG170000 Rh5CG227100
rosa_wichuraiana	Rw1G000740 Rw1G000780 Rw1G000810 Rw1G000840 Rw1G000870 Rw1G000900 Rw5G013560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	560
AccII	CGCG	1 cut(s)	282
AciI	CCGC	5 cut(s)	39, 280, 508, 899, 1390
AclWI	GGATC	1 cut(s)	701
AcoI	YGGCCR	2 cut(s)	509, 1034
AcsI	RAATTY	1 cut(s)	591
AcuI	CTGAAG	3 cut(s)	81, 1259, 1440
AcvI	CACGTG	1 cut(s)	17
AfaI	GTAC	5 cut(s)	330, 393, 759, 1012, 1296
AfiI	CCNNNNNNNGG	1 cut(s)	116
AflII	CTTAAG	1 cut(s)	653
AflIII	ACRYGT	2 cut(s)	14, 16
AgsI	TTSAA	5 cut(s)	469, 644, 671, 746, 996
AjiI	CACGTC	1 cut(s)	1109
AjnI	CCWGG	2 cut(s)	840, 1057
Alw21I	GWGCWC	2 cut(s)	687, 1178
Alw26I	GTCTC	3 cut(s)	25, 97, 1362
Alw44I	GTGCAC	1 cut(s)	1174
AlwI	GGATC	1 cut(s)	701
AlwNI	CAGNNNCTG	1 cut(s)	1280
AoxI	GGCC	3 cut(s)	509, 752, 1034
ApaLI	GTGCAC	1 cut(s)	1174
ApeKI	GCWGC	8 cut(s)	254, 578, 691, 694, 890, 893, 896, 1247
ApoI	RAATTY	1 cut(s)	591
ArsI	GACNNNNNNTTYG	2 cut(s)	1226, 1258
AseI	ATTAAT	2 cut(s)	1304, 1322
Asp700I	GAANNNNTTC	1 cut(s)	34
AspS9I	GGNCC	1 cut(s)	752
AsuC2I	CCSGG	2 cut(s)	251, 414
AsuHPI	GGTGA	1 cut(s)	230
AsuII	TTCGAA	1 cut(s)	1232
BaeGI	GKGCMC	2 cut(s)	350, 1178
BaeI	ACNNNNGTAYC	2 cut(s)	389, 422
BalI	TGGCCA	1 cut(s)	1036
BanII	GRGCYC	2 cut(s)	251, 1263
BbrPI	CACGTG	1 cut(s)	17
Bbv12I	GWGCWC	2 cut(s)	687, 1178
BbvCI	CCTCAGC	1 cut(s)	363
BbvI	GCAGC	8 cut(s)	266, 565, 703, 706, 877, 880, 883, 1259
BccI	CCATC	7 cut(s)	110, 209, 215, 283, 418, 479, 953
BciT130I	CCWGG	2 cut(s)	842, 1059
BcnI	CCSGG	2 cut(s)	251, 414
BcoDI	GTCTC	3 cut(s)	25, 97, 1362
BfaI	CTAG	3 cut(s)	267, 357, 782
BfmI	CTRYAG	1 cut(s)	582
BfrI	CTTAAG	1 cut(s)	653
BisI	GCNGC	9 cut(s)	255, 509, 579, 692, 695, 891, 894, 897, 1248
BlsI	GCNGC	9 cut(s)	256, 510, 580, 693, 696, 892, 895, 898, 1249
Bme1390I	CCNGG	4 cut(s)	251, 414, 842, 1059
BmgBI	CACGTC	1 cut(s)	1109
BmgT120I	GGNCC	1 cut(s)	752
BmiI	GGNNCC	1 cut(s)	838
BmrFI	CCNGG	4 cut(s)	251, 414, 842, 1059
BmsI	GCATC	8 cut(s)	286, 399, 453, 637, 943, 1186, 1333, 1462
Bpu10I	CCTNAGC	1 cut(s)	363
Bpu14I	TTCGAA	1 cut(s)	1232
BpuEI	CTTGAG	3 cut(s)	251, 924, 1247
BpuMI	CCSGG	2 cut(s)	251, 414
Bsa29I	ATCGAT	1 cut(s)	483
BsaAI	YACGTR	2 cut(s)	17, 1088
BsaBI	GATNNNNATC	2 cut(s)	153, 225
BsaI	GGTCTC	1 cut(s)	97
BsaJI	CCNNGG	2 cut(s)	840, 841
Bsc4I	CCNNNNNNNGG	1 cut(s)	116
Bse118I	RCCGGY	1 cut(s)	511
Bse1I	ACTGG	2 cut(s)	599, 765
Bse3DI	GCAATG	2 cut(s)	313, 1353
Bse8I	GATNNNNATC	2 cut(s)	153, 225
BseBI	CCWGG	2 cut(s)	842, 1059
BseCI	ATCGAT	1 cut(s)	483
BseDI	CCNNGG	2 cut(s)	840, 841
BseGI	GGATG	5 cut(s)	294, 301, 934, 1294, 1441
BseJI	GATNNNNATC	2 cut(s)	153, 225
BseLI	CCNNNNNNNGG	1 cut(s)	116
BseMI	GCAATG	2 cut(s)	313, 1353
BseMII	CTCAG	3 cut(s)	354, 712, 1212
BseNI	ACTGG	2 cut(s)	599, 765
BseRI	GAGGAG	3 cut(s)	156, 695, 1413
BseSI	GKGCMC	2 cut(s)	350, 1178
BseX3I	CGGCCG	1 cut(s)	509
BseXI	GCAGC	8 cut(s)	266, 565, 703, 706, 877, 880, 883, 1259
Bsh1236I	CGCG	1 cut(s)	282
Bsh1285I	CGRYCG	1 cut(s)	512
BshFI	GGCC	3 cut(s)	511, 754, 1036
BshVI	ATCGAT	1 cut(s)	483
BsiEI	CGRYCG	1 cut(s)	512
BsiHKAI	GWGCWC	2 cut(s)	687, 1178
BsiSI	CCGG	3 cut(s)	251, 413, 512
BslFI	GGGAC	2 cut(s)	33, 1119
BslI	CCNNNNNNNGG	1 cut(s)	116
BsmAI	GTCTC	3 cut(s)	25, 97, 1362
BsmFI	GGGAC	2 cut(s)	33, 1119
BsmI	GAATGC	1 cut(s)	1474
BsnI	GGCC	3 cut(s)	511, 754, 1036
Bso31I	GGTCTC	1 cut(s)	97
Bsp119I	TTCGAA	1 cut(s)	1232
Bsp1286I	GDGCHC	5 cut(s)	251, 350, 687, 1178, 1263
Bsp1407I	TGTACA	1 cut(s)	328
Bsp143I	GATC	2 cut(s)	706, 1183
BspACI	CCGC	5 cut(s)	39, 280, 508, 899, 1390
BspANI	GGCC	3 cut(s)	511, 754, 1036
BspCNI	CTCAG	3 cut(s)	355, 711, 1213
BspDI	ATCGAT	1 cut(s)	483
BspFNI	CGCG	1 cut(s)	282
BspHI	TCATGA	2 cut(s)	243, 877
BspLI	GGNNCC	1 cut(s)	838
BspPI	GGATC	1 cut(s)	701
BspT104I	TTCGAA	1 cut(s)	1232
BspTI	CTTAAG	1 cut(s)	653
BspTNI	GGTCTC	1 cut(s)	97
BsrDI	GCAATG	2 cut(s)	313, 1353
BsrFI	RCCGGY	1 cut(s)	511
BsrGI	TGTACA	1 cut(s)	328
BsrI	ACTGG	2 cut(s)	599, 765
BssAI	RCCGGY	1 cut(s)	511
BssECI	CCNNGG	2 cut(s)	840, 841
BssMI	GATC	2 cut(s)	706, 1183
Bst2UI	CCWGG	2 cut(s)	842, 1059
Bst4CI	ACNGT	5 cut(s)	7, 402, 859, 1015, 1078
Bst6I	CTCTTC	1 cut(s)	100
BstAFI	CTTAAG	1 cut(s)	653
BstAUI	TGTACA	1 cut(s)	328
BstBAI	YACGTR	2 cut(s)	17, 1088
BstBI	TTCGAA	1 cut(s)	1232
BstC8I	GCNNGC	2 cut(s)	73, 849
BstDEI	CTNAG	4 cut(s)	363, 698, 1221, 1368
BstF5I	GGATG	5 cut(s)	294, 301, 934, 1294, 1441
BstFNI	CGCG	1 cut(s)	282
BstKTI	GATC	2 cut(s)	709, 1186
BstMAI	GTCTC	3 cut(s)	25, 97, 1362
BstMBI	GATC	2 cut(s)	706, 1183
BstMCI	CGRYCG	1 cut(s)	512
BstMWI	GCNNNNNNNGC	6 cut(s)	263, 584, 691, 896, 902, 1471
BstNI	CCWGG	2 cut(s)	842, 1059
BstNSI	RCATGY	1 cut(s)	851
BstSCI	CCNGG	4 cut(s)	249, 412, 840, 1057
BstSFI	CTRYAG	1 cut(s)	582
BstSLI	GKGCMC	2 cut(s)	350, 1178
BstUI	CGCG	1 cut(s)	282
BstV1I	GCAGC	8 cut(s)	266, 565, 703, 706, 877, 880, 883, 1259
BstZI	CGGCCG	1 cut(s)	509
Bsu15I	ATCGAT	1 cut(s)	483
BsuRI	GGCC	3 cut(s)	511, 754, 1036
BsuTUI	ATCGAT	1 cut(s)	483
BtrI	CACGTC	1 cut(s)	1109
BtsCI	GGATG	5 cut(s)	294, 301, 934, 1294, 1441
BtsI	GCAGTG	1 cut(s)	608
BtsIMutI	CAGTG	1 cut(s)	608
Cac8I	GCNNGC	2 cut(s)	73, 849
CaiI	CAGNNNCTG	1 cut(s)	1280
CciI	TCATGA	2 cut(s)	243, 877
Cfr10I	RCCGGY	1 cut(s)	511
Cfr13I	GGNCC	1 cut(s)	752
ClaI	ATCGAT	1 cut(s)	483
CseI	GACGC	1 cut(s)	863
Csp6I	GTAC	5 cut(s)	329, 392, 758, 1011, 1295
CviAII	CATG	9 cut(s)	244, 332, 848, 871, 878, 1102, 1193, 1340, 1470
CviQI	GTAC	5 cut(s)	329, 392, 758, 1011, 1295
DdeI	CTNAG	4 cut(s)	363, 698, 1221, 1368
DpnI	GATC	2 cut(s)	708, 1185
DpnII	GATC	2 cut(s)	706, 1183
EaeI	YGGCCR	2 cut(s)	509, 1034
EagI	CGGCCG	1 cut(s)	509
Eam1104I	CTCTTC	1 cut(s)	100
EarI	CTCTTC	1 cut(s)	100
EciI	GGCGGA	1 cut(s)	28
EclXI	CGGCCG	1 cut(s)	509
Eco24I	GRGCYC	2 cut(s)	251, 1263
Eco31I	GGTCTC	1 cut(s)	97
Eco32I	GATATC	1 cut(s)	1026
Eco52I	CGGCCG	1 cut(s)	509
Eco57I	CTGAAG	3 cut(s)	81, 1259, 1440
Eco72I	CACGTG	1 cut(s)	17
EcoO109I	RGGNCCY	1 cut(s)	752
EcoRII	CCWGG	2 cut(s)	840, 1057
EcoRV	GATATC	1 cut(s)	1026
EcoT38I	GRGCYC	2 cut(s)	251, 1263
FaeI	CATG	9 cut(s)	247, 335, 851, 874, 881, 1105, 1196, 1343, 1473
FalI	AAGNNNNNCTT	2 cut(s)	636, 668
FaqI	GGGAC	2 cut(s)	33, 1119
FatI	CATG	9 cut(s)	243, 331, 847, 870, 877, 1101, 1192, 1339, 1469
FblI	GTMKAC	1 cut(s)	560
Fnu4HI	GCNGC	9 cut(s)	255, 509, 579, 692, 695, 891, 894, 897, 1248
FokI	GGATG	5 cut(s)	301, 308, 921, 1301, 1448
FriOI	GRGCYC	2 cut(s)	251, 1263
Fsp4HI	GCNGC	9 cut(s)	255, 509, 579, 692, 695, 891, 894, 897, 1248
FspBI	CTAG	3 cut(s)	267, 357, 782
GluI	GCNGC	9 cut(s)	255, 509, 579, 692, 695, 891, 894, 897, 1248
HaeIII	GGCC	3 cut(s)	511, 754, 1036
HapII	CCGG	3 cut(s)	251, 413, 512
HgaI	GACGC	1 cut(s)	863
Hin1II	CATG	9 cut(s)	247, 335, 851, 874, 881, 1105, 1196, 1343, 1473
HinfI	GANTC	3 cut(s)	736, 986, 1217
HpaII	CCGG	3 cut(s)	251, 413, 512
HphI	GGTGA	1 cut(s)	230
Hpy166II	GTNNAC	2 cut(s)	561, 1176
Hpy188I	TCNGA	3 cut(s)	127, 701, 1222
Hpy188III	TCNNGA	8 cut(s)	158, 230, 244, 644, 878, 941, 1264, 1286
Hpy8I	GTNNAC	2 cut(s)	561, 1176
HpyAV	CCTTC	2 cut(s)	463, 536
HpyCH4III	ACNGT	5 cut(s)	7, 402, 859, 1015, 1078
HpyCH4IV	ACGT	3 cut(s)	16, 1087, 1108
HpyF10VI	GCNNNNNNNGC	6 cut(s)	263, 584, 691, 896, 902, 1471
HpyF3I	CTNAG	4 cut(s)	363, 698, 1221, 1368
HpySE526I	ACGT	3 cut(s)	16, 1087, 1108
Hsp92II	CATG	9 cut(s)	247, 335, 851, 874, 881, 1105, 1196, 1343, 1473
Kzo9I	GATC	2 cut(s)	706, 1183
LmnI	GCTCC	3 cut(s)	682, 1313, 1331
Lsp1109I	GCAGC	8 cut(s)	266, 565, 703, 706, 877, 880, 883, 1259
LweI	GCATC	8 cut(s)	286, 399, 453, 637, 943, 1186, 1333, 1462
MaeI	CTAG	3 cut(s)	267, 357, 782
MaeII	ACGT	3 cut(s)	16, 1087, 1108
MaeIII	GTNAC	7 cut(s)	10, 634, 673, 823, 974, 1066, 1409
MalI	GATC	2 cut(s)	708, 1185
MboI	GATC	2 cut(s)	706, 1183
MboII	GAAGA	3 cut(s)	87, 551, 1433
MfeI	CAATTG	2 cut(s)	1092, 1179
MhlI	GDGCHC	5 cut(s)	251, 350, 687, 1178, 1263
MlsI	TGGCCA	1 cut(s)	1036
MluCI	AATT	6 cut(s)	491, 591, 639, 1092, 1127, 1179
MluNI	TGGCCA	1 cut(s)	1036
Mox20I	TGGCCA	1 cut(s)	1036
MroXI	GAANNNNTTC	1 cut(s)	34
MscI	TGGCCA	1 cut(s)	1036
MseI	TTAA	5 cut(s)	654, 792, 1304, 1322, 1364
MslI	CAYNNNNRTG	1 cut(s)	452
Msp20I	TGGCCA	1 cut(s)	1036
MspA1I	CMGCKG	2 cut(s)	257, 1392
MspCI	CTTAAG	1 cut(s)	653
MspI	CCGG	3 cut(s)	251, 413, 512
MspR9I	CCNGG	4 cut(s)	251, 414, 842, 1059
MunI	CAATTG	2 cut(s)	1092, 1179
Mva1269I	GAATGC	1 cut(s)	1474
MvaI	CCWGG	2 cut(s)	842, 1059
MvnI	CGCG	1 cut(s)	282
MwoI	GCNNNNNNNGC	6 cut(s)	263, 584, 691, 896, 902, 1471
NciI	CCSGG	2 cut(s)	251, 414
NdeII	GATC	2 cut(s)	706, 1183
NlaIII	CATG	9 cut(s)	247, 335, 851, 874, 881, 1105, 1196, 1343, 1473
NlaIV	GGNNCC	1 cut(s)	838
NmuCI	GTSAC	2 cut(s)	634, 1409
NspI	RCATGY	1 cut(s)	851
NspV	TTCGAA	1 cut(s)	1232
PaeI	GCATGC	1 cut(s)	851
PagI	TCATGA	2 cut(s)	243, 877
PasI	CCCWGGG	1 cut(s)	841
PctI	GAATGC	1 cut(s)	1474
PdmI	GAANNNNTTC	1 cut(s)	34
PfeI	GAWTC	3 cut(s)	736, 986, 1217
PfoI	TCCNGGA	1 cut(s)	1057
PkrI	GCNGC	9 cut(s)	256, 510, 580, 693, 696, 892, 895, 898, 1249
PmaCI	CACGTG	1 cut(s)	17
PmlI	CACGTG	1 cut(s)	17
Ppu21I	YACGTR	2 cut(s)	17, 1088
PshBI	ATTAAT	2 cut(s)	1304, 1322
Psp6I	CCWGG	2 cut(s)	840, 1057
PspCI	CACGTG	1 cut(s)	17
PspGI	CCWGG	2 cut(s)	840, 1057
PspN4I	GGNNCC	1 cut(s)	838
PspPI	GGNCC	1 cut(s)	752
PstNI	CAGNNNCTG	1 cut(s)	1280
PvuII	CAGCTG	1 cut(s)	257
RsaI	GTAC	5 cut(s)	330, 393, 759, 1012, 1296
RsaNI	GTAC	5 cut(s)	329, 392, 758, 1011, 1295
RseI	CAYNNNNRTG	1 cut(s)	452
SaqAI	TTAA	5 cut(s)	654, 792, 1304, 1322, 1364
SatI	GCNGC	9 cut(s)	255, 509, 579, 692, 695, 891, 894, 897, 1248
Sau3AI	GATC	2 cut(s)	706, 1183
Sau96I	GGNCC	1 cut(s)	752
ScrFI	CCNGG	4 cut(s)	251, 414, 842, 1059
SduI	GDGCHC	5 cut(s)	251, 350, 687, 1178, 1263
SfaNI	GCATC	8 cut(s)	286, 399, 453, 637, 943, 1186, 1333, 1462
SfcI	CTRYAG	1 cut(s)	582
SfuI	TTCGAA	1 cut(s)	1232
SmiMI	CAYNNNNRTG	1 cut(s)	452
SmlI	CTYRAG	4 cut(s)	230, 653, 939, 1262
SmoI	CTYRAG	4 cut(s)	230, 653, 939, 1262
SphI	GCATGC	1 cut(s)	851
Sse9I	AATT	6 cut(s)	491, 591, 639, 1092, 1127, 1179
SsiI	CCGC	5 cut(s)	39, 280, 508, 899, 1390
SspI	AATATT	1 cut(s)	165
SspMI	CTAG	3 cut(s)	267, 357, 782
StyD4I	CCNGG	4 cut(s)	249, 412, 840, 1057
TaaI	ACNGT	5 cut(s)	7, 402, 859, 1015, 1078
TaiI	ACGT	3 cut(s)	19, 1090, 1111
TaqI	TCGA	5 cut(s)	337, 483, 1028, 1232, 1396
TasI	AATT	6 cut(s)	491, 591, 639, 1092, 1127, 1179
TatI	WGTACW	2 cut(s)	328, 757
TauI	GCSGC	1 cut(s)	511
TfiI	GAWTC	3 cut(s)	736, 986, 1217
Tru1I	TTAA	5 cut(s)	654, 792, 1304, 1322, 1364
Tru9I	TTAA	5 cut(s)	654, 792, 1304, 1322, 1364
TscAI	CASTG	1 cut(s)	615
TseFI	GTSAC	2 cut(s)	634, 1409
TseI	GCWGC	8 cut(s)	254, 578, 691, 694, 890, 893, 896, 1247
Tsp45I	GTSAC	2 cut(s)	634, 1409
TspDTI	ATGAA	8 cut(s)	182, 189, 232, 302, 552, 556, 1305, 1467
TspGWI	ACGGA	1 cut(s)	46
TspRI	CASTG	1 cut(s)	615
Vha464I	CTTAAG	1 cut(s)	653
VneI	GTGCAC	1 cut(s)	1174
VspI	ATTAAT	2 cut(s)	1304, 1322
XapI	RAATTY	1 cut(s)	591
XceI	RCATGY	1 cut(s)	851
XcmI	CCANNNNNNNNNTGG	3 cut(s)	58, 717, 848
XmiI	GTMKAC	1 cut(s)	560
XmnI	GAANNNNTTC	1 cut(s)	34
XspI	CTAG	3 cut(s)	267, 357, 782

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.