Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0000685

Physical Location & Seq

Genomic Coordinates Forward (+)

5778122 .. 5783067

Gene Structure 4946 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0067300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3003 bp

ATGAAGACAAAAGTGCTTCTCTTCTTCCTCCTCCCTCTCCTCCTCTTCTCCTCCACCTCCTCCTCCTCCTCCTCCCCACTCACCCAAGAAGGCCTCTACCTCCAACACATCAAACACTCCCTCGCCGACCCCGACTCCTCCCTCTCCTCCTGGAACGACCAAGACGCCACCCCTTGCTCATGGGACGGCATCACATGCAACCCTTCCACCCACGTCATCACCTCCGTCGACCTCTCCAGCTTCAACCTCTTCGGCCCTTTCCCCTCCCTCCTCTGCCGCCTCCCCAACCTCACCTCCCTCTCCCTCTACAACAACTCCATCAACTCCACCCTACCCTCCGACATCTCCACGTGTCAGAACCTCCAACACCTCGACCTCTCCCAGAACCTCCTCACCGGCTCCCTCCCCTCCACCCTTGCCGACCTCCCCAACCTCCGCTACCTCGACCTCACCGGCAACAACTTCTCGGGAGAAATTCCCGAGTCTTTCGGGAGATTCCAAAAACTCGAGGTCCTCTCTCTCGTCTACAACCTCCTCGACAGCGCCCTGCCCCCATTTCTCGGCAATATCTCGTCCCTCAAAATGCTCAACCTTTCTTACAACCCGTTCTTCCCGGGTCGGATCCCGCCCGAGCTCGGAAACTTAACGAACCTCGAGGTCCTCTGGCTGACCGAGTGTAACTTAGTCGGCGAAATACCCGACTCGCTGGGTCGACTCAGTAAACTCACCGATTTGGACCTCGCCATTAACGCCCTTCACGGCCCGATTCCGGCTTCGTTGACGGAGTTGACCAGCGTGGTTCAGATTGAGCTCTATAACAACACCTTGACCGGAGCCCTGCCGCCGGGAATGTCGAAGCTGAAGAAGCTCCGGCTGCTCGACGCGTCCATGAACCGGCTGACGGGGCCGATTCCCGACGAGTTGACTCGGCTGGAGCTGGAAAGCCTGAATCTTTACGAGAACAACTTCGAGGGGAGTTTGCCGGCGAGCATTGCCGACTCGCCGAACCTGTACGAGCTCCGGCTGTTTCAGAACAAGCTCACCGGCGAGCTGCCGCGGAATCTCGGCCAGAACTCGCCGCTGAGGTGGATAGACCTGTCGAACAACCAGTTTTCAGGGAGCATTCCGGCGAGTCTGTGCGAGAAGGGGCAAATTGAGGAGATGCTGATGATCAACAATGCTTTTTCCGGCGAGATTCCGGCGAGTCTCGGGGAGTGTCAGAGCTTGACACGTGTCCGGTTGGGTCACAACCGGTTGAGCGGTGAAGTTCCGGCGTCTTTCTGGGGGCTGCCGCATGTCTATTTGCTCGAGCTGGTTGATAACCAGTTATCGGGGCCGATAGCCAAGACAATTGCCGGTGCGGCTAACCTTTCGCTTCTGATAATCGAAAAGAACCAGTTTGAAGGTTCGATACCGGAGGAAATTGGGTGGGTTGAAAACCTTCTGCAGTTTTCCGGCGGTGAGAATAGGTTCAGTGGGCCTTTGCCGGAGACAATAGTTAAGTTACGACATCTTAGCACGCTGGACCTTCACAACAATGAGCTTTCCGGTGAGTTTCCGATTGGGAGTCAGTCTTTGGTTCGGCTCAATGAGCTCAATTTGAGGAATAACCAACTGTCCGGGAAAATCCCAGATGGAATTGGGAACATGTCTTCGCTGAATTACCTTGATCTCTCCGGGAATCGGTTTTCAGGTCAAGTTCCGGTTTGGTTGCAGAATATGAAGATCAACGTGTTCAATTTGTCGGATAACCAGCTTTCGGGTGAGCTCCCTCCTTTGTTTGCGAAGGAGATGTACAGAAATAGCTTTCTGGGAAATCCGGGTCTTTGCGGAGATTTGGAGGGGTTGTGTGAGAGCAGAGCCGAGGAGAAGACCCAGGGCTACATATGGTTGCTGAGGTGCATTTTCATTCTGGCTGCCTTGGTGTTTGTGGTGGGTGTGGTTTGGTTTTACTTCAAGTACAAGAACTTCAAGAAGGCCAGCGGTGCTATAGATAAATCCAAGTGGACTTTGATGTCGTTTCACAAGCTGGGTTTCAGCGAGTATGAGATACTGGATTGCCTTGATGAGGATAATGTGATTGGGACTGGGGGGTCTGGGAAAGTGTACAAGGTTGTGCTGAGCAATGGTGAGGCTGTTGCGGTGAAAAAGCTGTGGAGGGGGAAAGTGAAAGAGTGCGAAAGCGATGATGTTGAGAAAGGTTGGGTCCAAGATGATGGCTTTGAGGCAGAGGTCGACACTTTGGGGAGGATTAGGCACAAGAATATTGTGAAGCTTTGGTGCTGTTGTACCGCAAGAGATTGCAAGCTTTTGGTGTATGAGTACATGCCAAATGGTAGTCTTGGTGATTTGCTGCATAGTAGCAAAGCAGGGTTGCTGGATTGGCCTACAAGGTTCAAGATTGCTCTTGATGCTGCTGATGGGCTTTCGTATCTGCACCATGATTGTGTTCCTGCTATTGTTCATAGAGATGTGAAATCAAACAATATATTGTTGGATGGGGATTTCGGAGCCAGAGTAGCGGATTTTGGAGTAGCTAAGGTCGTTGATGCAACTGGAAAAGGGGCTAAATCCATGTCGGTCATTGCAGGTTCTTGTGGTTACATTGCTCCAGAATATGCATACACACTGAGGGTGAATGAGAAGAGTGACATCTACAGTTTTGGTGTGGTGATTCTGGAATTGGTTACCGGGAAACTTCCAGTTGACCCAGAATTCGGAGAAAAGGACTTGGTGAAGTGGGTCTGTACCACCTTAGATCAGAAAGGAGTGGACCATGTCATTGACCCCAAACTCGATTCTTGTTACAAGGAAGAAGTATGCAAGGTCCTCAATATCGGCCTCCTCTGCACCAGTCCTCTCCCAATCAATAGACCATCGATGAGAAGAGTGGTGAAGTTGTTGCAAGAAGCGGGAACGGAGAAGCATCCTCAGATTAAGAAGGAAGGCAAATTGTCACCATATTACTATGAAGATGCATCGGATCATGGAAGTGTAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000255 GO:0000256 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009987 GO:0010135 GO:0010136 GO:0012505 GO:0016787 GO:0016810 GO:0016813 GO:0017144 GO:0019439 GO:0034641 GO:0042737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043605 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046113 GO:0046483 GO:0046700 GO:0047652 GO:0055086 GO:0071704 GO:0072521 GO:0072523 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00230 ko01120 map00230 map01120

Pfam Domains

Protein Families

M20_dimer Peptidase_M20 Peptidase_M28

Protein Analysis

1000

Amino Acids

110.22

Weight (kDa)

5.24

Isoelectric Point (pI)

36.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	28 - 67	1.3e-12	Leucine rich repeat N-terminal domain
LRR_Zer-1	PF25013	73 - 158	7.9e-06	Zer-1-like, Leucine-rich repeats
LRR_14	PF23598	91 - 196	3.1e-08	Leucine-rich repeat region
LRR_8	PF13855	95 - 155	4.4e-11	Leucine rich repeat
LRR_4	PF12799	119 - 159	9.2e-09	Leucine Rich repeats (2 copies)
LRR_14	PF23598	186 - 345	5.8e-09	Leucine-rich repeat region
LRR_14	PF23598	494 - 559	1.1e-07	Leucine-rich repeat region
Pkinase	PF00069	691 - 916	2.9e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	691 - 966	1.3e-35	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0000685)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20070 AT4G20070
fragaria_vesca	FvH4_3g12850 FvH4_3g12850 FvH4_3g12850
malus_domestica	MD10G1228200.v1.1
prunus_persica	Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1 Prupe.4G116700_v2.0.a1
pyrus_communis	pycom10g19170
rosa_chinensis	RchiOBHm_Chr1g0315031 RchiOBHm_Chr1g0315061 RchiOBHm_Chr1g0315091 RchiOBHm_Chr1g0315141 RchiOBHm_Chr1g0315151 RchiOBHm_Chr1g0315201 RchiOBHm_Chr1g0315231 RchiOBHm_Chr1g0315241 RchiOBHm_Chr1g0315311 RchiOBHm_Chr1g0315381 RchiOBHm_Chr1g0315441 RchiOBHm_Chr1g0315471 RchiOBHm_Chr1g0315531 RchiOBHm_Chr1g0315541 RchiOBHm_Chr1g0315581 RchiOBHm_Chr1g0315591 RchiOBHm_Chr1g0315611 RchiOBHm_Chr5g0020791 RchiOBHm_Chr5g0020891 RchiOBHm_Chr5g0021441 RchiOBHm_Chr5g0021511 RchiOBHm_Chr5g0021521 RchiOBHm_Chr5g0029451 RchiOBHm_Chr5g0029471
rosa_laevigata	RLG00000030744 RLG00000030748 RLG00000032563 RLG00000032611
rosa_multiflora	Rmu_sc0000576.1_g000021 Rmu_sc0002052.1_g000003 Rmu_sc0004180.1_g000017 Rmu_sc0004180.1_g000030 Rmu_sc0004180.1_g000035 Rmu_sc0023070.1_g000002 Rmu_ssc0000244.1_g000002
rosa_roxburghii	Rroxscaffold_1G00057130 Rroxscaffold_1G00057510 Rroxscaffold_4G00331580
rosa_rugosa	Rorug01G0004300 Rorug05G0063000 Rorug05G0067300
rosa_samantha	Rh1AG015000 Rh1AG015600 Rh1BG007400 Rh1BG007800 Rh1CG013200 Rh5AG152900 Rh5BG152700 Rh5BG155800 Rh5CG164200 Rh5CG164700 Rh5CG170000 Rh5CG227100
rosa_wichuraiana	Rw1G000740 Rw1G000780 Rw1G000810 Rw1G000840 Rw1G000870 Rw1G000900 Rw5G013560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	2014, 2092
AbsI	CCTCGAGG	1 cut(s)	653
Acc36I	ACCTGC	1 cut(s)	2579
AccBSI	CCGCTC	1 cut(s)	1260
AccI	GTMKAC	4 cut(s)	228, 525, 712, 2235
AccII	CGCG	2 cut(s)	884, 1057
AclWI	GGATC	3 cut(s)	616, 629, 2991
AcoI	YGGCCR	1 cut(s)	1066
AcsI	RAATTY	2 cut(s)	474, 2714
AcuI	CTGAAG	1 cut(s)	881
AcvI	CACGTG	2 cut(s)	351, 1232
AcyI	GRCGYC	2 cut(s)	165, 1274
AfaI	GTAC	7 cut(s)	1013, 1796, 1961, 2108, 2290, 2324, 2749
AflIII	ACRYGT	6 cut(s)	350, 882, 1229, 1231, 1647, 1731
AgeI	ACCGGT	1 cut(s)	1251
AgsI	TTSAA	7 cut(s)	244, 1403, 1436, 1738, 1957, 1972, 2398
AjiI	CACGTC	1 cut(s)	214
AjnI	CCWGG	2 cut(s)	149, 1875
Alw21I	GWGCWC	5 cut(s)	636, 813, 1020, 1596, 1770
Alw26I	GTCTC	2 cut(s)	1211, 1484
AlwI	GGATC	3 cut(s)	616, 629, 2991
Ama87I	CYCGRG	8 cut(s)	466, 479, 506, 613, 629, 653, 1208, 1307
ApeKI	GCWGC	6 cut(s)	874, 1051, 1288, 1916, 2353, 2414
ApoI	RAATTY	2 cut(s)	474, 2714
ArsI	GACNNNNNNTTYG	2 cut(s)	2699, 2731
AsiGI	ACCGGT	1 cut(s)	1251
Asp700I	GAANNNNTTC	3 cut(s)	965, 1440, 1469
AspLEI	GCGC	1 cut(s)	545
AsuC2I	CCSGG	7 cut(s)	614, 615, 846, 1621, 1678, 1821, 2692
AvaI	CYCGRG	8 cut(s)	466, 479, 506, 613, 629, 653, 1208, 1307
AvaII	GGWCC	7 cut(s)	511, 658, 736, 1525, 2206, 2773, 2827
BamHI	GGATCC	1 cut(s)	621
BanII	GRGCYC	6 cut(s)	636, 813, 838, 1020, 1596, 1770
BarI	GAAGNNNNNNTAC	6 cut(s)	1395, 1427, 1778, 1810, 1952, 1984
BbrPI	CACGTG	2 cut(s)	351, 1232
BbsI	GAAGAC	3 cut(s)	11, 1644, 1877
Bbv12I	GWGCWC	5 cut(s)	636, 813, 1020, 1596, 1770
BbvCI	CCTCAGC	2 cut(s)	1082, 1895
BbvI	GCAGC	6 cut(s)	861, 1038, 1275, 1903, 2340, 2401
BccI	CCATC	6 cut(s)	326, 1628, 2210, 2414, 2492, 2884
BceAI	ACGGC	2 cut(s)	202, 775
BciT130I	CCWGG	2 cut(s)	151, 1877
BclI	TGATCA	1 cut(s)	1170
BcnI	CCSGG	7 cut(s)	614, 615, 846, 1621, 1678, 1821, 2692
BcoDI	GTCTC	2 cut(s)	1211, 1484
BfmI	CTRYAG	3 cut(s)	1445, 1989, 2656
BfoI	RGCGCY	1 cut(s)	546
BfuAI	ACCTGC	1 cut(s)	2579
BglI	GCCNNNNNGGC	1 cut(s)	1361
BlpI	GCTNAGC	1 cut(s)	2120
Bme1390I	CCNGG	9 cut(s)	151, 614, 615, 846, 1621, 1678, 1821, 1877, 2692
Bme18I	GGWCC	7 cut(s)	511, 658, 736, 1525, 2206, 2773, 2827
BmeT110I	CYCGRG	8 cut(s)	466, 479, 506, 613, 629, 653, 1208, 1307
BmgBI	CACGTC	1 cut(s)	214
BmiI	GGNNCC	7 cut(s)	400, 623, 835, 906, 1335, 2207, 2512
BmrFI	CCNGG	9 cut(s)	151, 614, 615, 846, 1621, 1678, 1821, 1877, 2692
BmrI	ACTGGG	1 cut(s)	2097
BmsI	GCATC	7 cut(s)	198, 1152, 2401, 2539, 2935, 2965, 2987
BmuI	ACTGGG	1 cut(s)	2097
BoxI	GACNNNNGTC	1 cut(s)	1232
BpiI	GAAGAC	3 cut(s)	11, 1644, 1877
BplI	GAGNNNNNCTC	2 cut(s)	500, 532
BpmI	CTGGAG	3 cut(s)	220, 953, 2595
Bpu10I	CCTNAGC	3 cut(s)	1082, 1895, 2538
Bpu1102I	GCTNAGC	1 cut(s)	2120
BpuMI	CCSGG	7 cut(s)	614, 615, 846, 1621, 1678, 1821, 2692
Bsa29I	ATCGAT	1 cut(s)	2879
BsaAI	YACGTR	2 cut(s)	351, 1232
BsaHI	GRCGYC	2 cut(s)	165, 1274
BsaJI	CCNNGG	6 cut(s)	613, 1055, 1863, 1875, 1876, 1920
BsaWI	WCCGGW	6 cut(s)	830, 1236, 1251, 1414, 1547, 1702
Bse118I	RCCGGY	7 cut(s)	395, 452, 894, 982, 1043, 1251, 1355
Bse1I	ACTGG	8 cut(s)	1108, 1324, 1396, 2058, 2092, 2560, 2702, 2853
Bse3DI	GCAATG	4 cut(s)	990, 2131, 2583, 2604
BseBI	CCWGG	2 cut(s)	151, 1877
BseCI	ATCGAT	1 cut(s)	2879
BseDI	CCNNGG	6 cut(s)	613, 1055, 1863, 1875, 1876, 1920
BseGI	GGATG	2 cut(s)	2503, 2926
BseMI	GCAATG	4 cut(s)	990, 2131, 2583, 2604
BseMII	CTCAG	6 cut(s)	730, 1073, 1886, 2111, 2621, 2945
BseNI	ACTGG	8 cut(s)	1108, 1324, 1396, 2058, 2092, 2560, 2702, 2853
BseXI	GCAGC	6 cut(s)	861, 1038, 1275, 1903, 2340, 2401
BseYI	CCCAGC	2 cut(s)	706, 2029
BsgI	GTGCAG	2 cut(s)	2420, 2833
Bsh1236I	CGCG	2 cut(s)	884, 1057
BshTI	ACCGGT	1 cut(s)	1251
BshVI	ATCGAT	1 cut(s)	2879
BsiHKAI	GWGCWC	5 cut(s)	636, 813, 1020, 1596, 1770
BsiHKCI	CYCGRG	8 cut(s)	466, 479, 506, 613, 629, 653, 1208, 1307
BslFI	GGGAC	3 cut(s)	197, 559, 2098
BsmAI	GTCTC	2 cut(s)	1211, 1484
BsmFI	GGGAC	3 cut(s)	197, 559, 2098
BsmI	GAATGC	1 cut(s)	1122
BsoBI	CYCGRG	8 cut(s)	466, 479, 506, 613, 629, 653, 1208, 1307
Bsp1286I	GDGCHC	6 cut(s)	636, 813, 838, 1020, 1596, 1770
Bsp1407I	TGTACA	2 cut(s)	1794, 2106
Bsp143I	GATC	6 cut(s)	621, 1170, 1669, 1725, 2758, 2983
Bsp1720I	GCTNAGC	1 cut(s)	2120
BspCNI	CTCAG	6 cut(s)	729, 1074, 1887, 2112, 2622, 2944
BspDI	ATCGAT	1 cut(s)	2879
BspFNI	CGCG	2 cut(s)	884, 1057
BspLI	GGNNCC	7 cut(s)	400, 623, 835, 906, 1335, 2207, 2512
BspMAI	CTGCAG	1 cut(s)	1449
BspMI	ACCTGC	1 cut(s)	2579
BspPI	GGATC	3 cut(s)	616, 629, 2991
BsrBI	CCGCTC	1 cut(s)	1260
BsrDI	GCAATG	4 cut(s)	990, 2131, 2583, 2604
BsrFI	RCCGGY	7 cut(s)	395, 452, 894, 982, 1043, 1251, 1355
BsrGI	TGTACA	2 cut(s)	1794, 2106
BsrI	ACTGG	8 cut(s)	1108, 1324, 1396, 2058, 2092, 2560, 2702, 2853
BssAI	RCCGGY	7 cut(s)	395, 452, 894, 982, 1043, 1251, 1355
BssECI	CCNNGG	6 cut(s)	613, 1055, 1863, 1875, 1876, 1920
BssMI	GATC	6 cut(s)	621, 1170, 1669, 1725, 2758, 2983
BssNI	GRCGYC	2 cut(s)	165, 1274
BssT1I	CCWWGG	1 cut(s)	1920
Bst2UI	CCWGG	2 cut(s)	151, 1877
Bst4CI	ACNGT	2 cut(s)	1617, 2660
Bst6I	CTCTTC	5 cut(s)	26, 50, 254, 2639, 2881
BstACI	GRCGYC	2 cut(s)	165, 1274
BstAPI	GCANNNNNTGC	1 cut(s)	195
BstAUI	TGTACA	2 cut(s)	1794, 2106
BstBAI	YACGTR	2 cut(s)	351, 1232
BstC8I	GCNNGC	6 cut(s)	984, 988, 1049, 1520, 1981, 2306
BstDSI	CCRYGG	1 cut(s)	1055
BstEII	GGTNACC	1 cut(s)	2686
BstENI	CCTNNNNNAGG	1 cut(s)	2066
BstF5I	GGATG	2 cut(s)	2503, 2926
BstFNI	CGCG	2 cut(s)	884, 1057
BstH2I	RGCGCY	1 cut(s)	546
BstHHI	GCGC	1 cut(s)	545
BstKTI	GATC	6 cut(s)	624, 1173, 1672, 1728, 2761, 2986
BstMAI	GTCTC	2 cut(s)	1211, 1484
BstMBI	GATC	6 cut(s)	621, 1170, 1669, 1725, 2758, 2983
BstNI	CCWGG	2 cut(s)	151, 1877
BstNSI	RCATGY	4 cut(s)	198, 1298, 1651, 2329
BstPAI	GACNNNNGTC	1 cut(s)	1232
BstPI	GGTNACC	1 cut(s)	2686
BstSCI	CCNGG	9 cut(s)	149, 612, 613, 844, 1619, 1676, 1819, 1875, 2690
BstSFI	CTRYAG	3 cut(s)	1445, 1989, 2656
BstUI	CGCG	2 cut(s)	884, 1057
BstV1I	GCAGC	6 cut(s)	861, 1038, 1275, 1903, 2340, 2401
BstV2I	GAAGAC	3 cut(s)	11, 1644, 1877
BstX2I	RGATCY	1 cut(s)	621
BstXI	CCANNNNNNTGG	1 cut(s)	2216
BstYI	RGATCY	1 cut(s)	621
Bsu15I	ATCGAT	1 cut(s)	2879
BsuTUI	ATCGAT	1 cut(s)	2879
BtgI	CCRYGG	1 cut(s)	1055
BtgZI	GCGATG	1 cut(s)	2199
BtrI	CACGTC	1 cut(s)	214
BtsCI	GGATG	2 cut(s)	2503, 2926
BtsIMutI	CAGTG	2 cut(s)	1480, 2627
BveI	ACCTGC	1 cut(s)	2579
Cac8I	GCNNGC	6 cut(s)	984, 988, 1049, 1520, 1981, 2306
CfoI	GCGC	1 cut(s)	545
Cfr10I	RCCGGY	7 cut(s)	395, 452, 894, 982, 1043, 1251, 1355
Cfr42I	CCGCGG	1 cut(s)	1058
Cfr9I	CCCGGG	1 cut(s)	613
ClaI	ATCGAT	1 cut(s)	2879
CseI	GACGC	4 cut(s)	173, 873, 890, 1263
Csp6I	GTAC	7 cut(s)	1012, 1795, 1960, 2107, 2289, 2323, 2748
CspAI	ACCGGT	1 cut(s)	1251
CviQI	GTAC	7 cut(s)	1012, 1795, 1960, 2107, 2289, 2323, 2748
DpnI	GATC	6 cut(s)	623, 1172, 1671, 1727, 2760, 2985
DpnII	GATC	6 cut(s)	621, 1170, 1669, 1725, 2758, 2983
DrdI	GACNNNNNNGTC	2 cut(s)	2014, 2092
DseDI	GACNNNNNNGTC	2 cut(s)	2014, 2092
EaeI	YGGCCR	1 cut(s)	1066
Eam1104I	CTCTTC	5 cut(s)	26, 50, 254, 2639, 2881
EarI	CTCTTC	5 cut(s)	26, 50, 254, 2639, 2881
Ecl136II	GAGCTC	5 cut(s)	634, 811, 1018, 1594, 1768
Eco130I	CCWWGG	1 cut(s)	1920
Eco147I	AGGCCT	1 cut(s)	93
Eco24I	GRGCYC	6 cut(s)	636, 813, 838, 1020, 1596, 1770
Eco47I	GGWCC	7 cut(s)	511, 658, 736, 1525, 2206, 2773, 2827
Eco53kI	GAGCTC	5 cut(s)	634, 811, 1018, 1594, 1768
Eco57I	CTGAAG	1 cut(s)	881
Eco72I	CACGTG	2 cut(s)	351, 1232
Eco88I	CYCGRG	8 cut(s)	466, 479, 506, 613, 629, 653, 1208, 1307
Eco91I	GGTNACC	1 cut(s)	2686
EcoICRI	GAGCTC	5 cut(s)	634, 811, 1018, 1594, 1768
EcoNI	CCTNNNNNAGG	1 cut(s)	2066
EcoO109I	RGGNCCY	3 cut(s)	511, 658, 2827
EcoO65I	GGTNACC	1 cut(s)	2686
EcoRI	GAATTC	1 cut(s)	2714
EcoRII	CCWGG	2 cut(s)	149, 1875
EcoT14I	CCWWGG	1 cut(s)	1920
EcoT22I	ATGCAT	2 cut(s)	2623, 2980
EcoT38I	GRGCYC	6 cut(s)	636, 813, 838, 1020, 1596, 1770
ErhI	CCWWGG	1 cut(s)	1920
FalI	AAGNNNNNCTT	1 cut(s)	2983
FaqI	GGGAC	3 cut(s)	197, 559, 2098
FauI	CCCGC	2 cut(s)	633, 2905
FauNDI	CATATG	1 cut(s)	1886
FbaI	TGATCA	1 cut(s)	1170
FblI	GTMKAC	4 cut(s)	228, 525, 712, 2235
FokI	GGATG	2 cut(s)	2510, 2913
FriOI	GRGCYC	6 cut(s)	636, 813, 838, 1020, 1596, 1770
GlaI	GCGC	1 cut(s)	544
GsaI	CCCAGC	2 cut(s)	710, 2033
GsuI	CTGGAG	3 cut(s)	220, 953, 2595
HaeII	RGCGCY	1 cut(s)	546
HgaI	GACGC	4 cut(s)	173, 873, 890, 1263
HhaI	GCGC	1 cut(s)	545
Hin1I	GRCGYC	2 cut(s)	165, 1274
Hin6I	GCGC	1 cut(s)	543
HinP1I	GCGC	1 cut(s)	543
HincII	GTYRAC	7 cut(s)	229, 713, 780, 789, 924, 2236, 2707
HindII	GTYRAC	7 cut(s)	229, 713, 780, 789, 924, 2236, 2707
HindIII	AAGCTT	2 cut(s)	2273, 2306
Hpy188III	TCNNGA	9 cut(s)	468, 479, 490, 914, 1972, 2398, 2408, 2612, 2678
Hpy99I	CGWCG	3 cut(s)	230, 884, 920
HpyCH4III	ACNGT	2 cut(s)	1617, 2660
HpyCH4IV	ACGT	4 cut(s)	213, 350, 1231, 1731
HpySE526I	ACGT	4 cut(s)	213, 350, 1231, 1731
Hsp92I	GRCGYC	2 cut(s)	165, 1274
HspAI	GCGC	1 cut(s)	543
KroI	GCCGGC	1 cut(s)	982
KroNI	GCCGGC	1 cut(s)	984
Ksp22I	TGATCA	1 cut(s)	1170
KspI	CCGCGG	1 cut(s)	1058
Kzo9I	GATC	6 cut(s)	621, 1170, 1669, 1725, 2758, 2983
LmnI	GCTCC	9 cut(s)	404, 833, 873, 934, 1023, 1119, 1773, 2510, 2614
Lsp1109I	GCAGC	6 cut(s)	861, 1038, 1275, 1903, 2340, 2401
LweI	GCATC	7 cut(s)	198, 1152, 2401, 2539, 2935, 2965, 2987
MaeII	ACGT	4 cut(s)	213, 350, 1231, 1731
MaeIII	GTNAC	8 cut(s)	677, 1244, 1503, 2600, 2648, 2686, 2804, 2955
MalI	GATC	6 cut(s)	623, 1172, 1671, 1727, 2760, 2985
MbiI	CCGCTC	1 cut(s)	1260
MboI	GATC	6 cut(s)	621, 1170, 1669, 1725, 2758, 2983
MfeI	CAATTG	1 cut(s)	1350
MflI	RGATCY	1 cut(s)	621
MhlI	GDGCHC	6 cut(s)	636, 813, 838, 1020, 1596, 1770
MluI	ACGCGT	1 cut(s)	882
MlyI	GAGTC	9 cut(s)	128, 491, 695, 708, 919, 992, 1141, 1213, 1576
MmeI	TCCRAC	6 cut(s)	127, 363, 388, 599, 1725, 2475
Mph1103I	ATGCAT	2 cut(s)	2623, 2980
MroNI	GCCGGC	1 cut(s)	982
MroXI	GAANNNNTTC	3 cut(s)	965, 1440, 1469
MseI	TTAA	5 cut(s)	644, 747, 1500, 2937, 3001
MslI	CAYNNNNRTG	4 cut(s)	1536, 2445, 2469, 2991
MspA1I	CMGCKG	3 cut(s)	1057, 1081, 1983
MspR9I	CCNGG	9 cut(s)	151, 614, 615, 846, 1621, 1678, 1821, 1877, 2692
MunI	CAATTG	1 cut(s)	1350
Mva1269I	GAATGC	1 cut(s)	1122
MvaI	CCWGG	2 cut(s)	151, 1877
MvnI	CGCG	2 cut(s)	884, 1057
NaeI	GCCGGC	1 cut(s)	984
NciI	CCSGG	7 cut(s)	614, 615, 846, 1621, 1678, 1821, 2692
NdeI	CATATG	1 cut(s)	1886
NdeII	GATC	6 cut(s)	621, 1170, 1669, 1725, 2758, 2983
NgoMIV	GCCGGC	1 cut(s)	982
NlaIV	GGNNCC	7 cut(s)	400, 623, 835, 906, 1335, 2207, 2512
NmeAIII	GCCGAG	4 cut(s)	540, 907, 1044, 1888
NmuCI	GTSAC	3 cut(s)	1244, 2648, 2955
NsiI	ATGCAT	2 cut(s)	2623, 2980
NspI	RCATGY	4 cut(s)	198, 1298, 1651, 2329
PaeR7I	CTCGAG	3 cut(s)	506, 653, 1307
PasI	CCCWGGG	1 cut(s)	1876
PceI	AGGCCT	1 cut(s)	93
PciI	ACATGT	1 cut(s)	1647
PcsI	WCGNNNNNNNCGW	1 cut(s)	129
PctI	GAATGC	1 cut(s)	1122
PdiI	GCCGGC	1 cut(s)	984
PdmI	GAANNNNTTC	3 cut(s)	965, 1440, 1469
PfeI	GAWTC	9 cut(s)	495, 766, 910, 949, 1060, 1195, 1681, 2674, 2798
PflFI	GACNNNGTC	1 cut(s)	2777
PfoI	TCCNGGA	3 cut(s)	149, 1619, 1676
PinAI	ACCGGT	1 cut(s)	1251
PleI	GAGTC	9 cut(s)	128, 490, 695, 708, 919, 992, 1140, 1212, 1575
PmaCI	CACGTG	2 cut(s)	351, 1232
PmlI	CACGTG	2 cut(s)	351, 1232
PpsI	GAGTC	9 cut(s)	128, 490, 695, 708, 919, 992, 1140, 1212, 1575
Ppu21I	YACGTR	2 cut(s)	351, 1232
PpuMI	RGGWCCY	3 cut(s)	511, 658, 2827
PscI	ACATGT	1 cut(s)	1647
PshAI	GACNNNNGTC	1 cut(s)	1232
Psp124BI	GAGCTC	5 cut(s)	636, 813, 1020, 1596, 1770
Psp5II	RGGWCCY	3 cut(s)	511, 658, 2827
Psp6I	CCWGG	2 cut(s)	149, 1875
PspCI	CACGTG	2 cut(s)	351, 1232
PspEI	GGTNACC	1 cut(s)	2686
PspFI	CCCAGC	2 cut(s)	706, 2029
PspGI	CCWGG	2 cut(s)	149, 1875
PspN4I	GGNNCC	7 cut(s)	400, 623, 835, 906, 1335, 2207, 2512
PspPPI	RGGWCCY	3 cut(s)	511, 658, 2827
PspXI	VCTCGAGB	3 cut(s)	506, 653, 1307
PstI	CTGCAG	1 cut(s)	1449
PsuI	RGATCY	1 cut(s)	621
PsyI	GACNNNGTC	1 cut(s)	2777
RsaI	GTAC	7 cut(s)	1013, 1796, 1961, 2108, 2290, 2324, 2749
RsaNI	GTAC	7 cut(s)	1012, 1795, 1960, 2107, 2289, 2323, 2748
RseI	CAYNNNNRTG	4 cut(s)	1536, 2445, 2469, 2991
SacI	GAGCTC	5 cut(s)	636, 813, 1020, 1596, 1770
SacII	CCGCGG	1 cut(s)	1058
SalI	GTCGAC	3 cut(s)	227, 711, 2234
SaqAI	TTAA	5 cut(s)	644, 747, 1500, 2937, 3001
Sau3AI	GATC	6 cut(s)	621, 1170, 1669, 1725, 2758, 2983
SchI	GAGTC	9 cut(s)	128, 491, 695, 708, 919, 992, 1141, 1213, 1576
ScrFI	CCNGG	9 cut(s)	151, 614, 615, 846, 1621, 1678, 1821, 1877, 2692
SduI	GDGCHC	6 cut(s)	636, 813, 838, 1020, 1596, 1770
SfaNI	GCATC	7 cut(s)	198, 1152, 2401, 2539, 2935, 2965, 2987
SfcI	CTRYAG	3 cut(s)	1445, 1989, 2656
Sfr274I	CTCGAG	3 cut(s)	506, 653, 1307
Sfr303I	CCGCGG	1 cut(s)	1058
SgrAI	CRCCGGYG	1 cut(s)	1043
SgrBI	CCGCGG	1 cut(s)	1058
SinI	GGWCC	7 cut(s)	511, 658, 736, 1525, 2206, 2773, 2827
SlaI	CTCGAG	3 cut(s)	506, 653, 1307
SmaI	CCCGGG	1 cut(s)	615
SmiMI	CAYNNNNRTG	4 cut(s)	1536, 2445, 2469, 2991
SmlI	CTYRAG	3 cut(s)	506, 653, 1307
SmoI	CTYRAG	3 cut(s)	506, 653, 1307
SseBI	AGGCCT	1 cut(s)	93
SspI	AATATT	1 cut(s)	2266
SstI	GAGCTC	5 cut(s)	636, 813, 1020, 1596, 1770
StuI	AGGCCT	1 cut(s)	93
StyD4I	CCNGG	9 cut(s)	149, 612, 613, 844, 1619, 1676, 1819, 1875, 2690
StyI	CCWWGG	1 cut(s)	1920
TaaI	ACNGT	2 cut(s)	1617, 2660
TaiI	ACGT	4 cut(s)	216, 353, 1234, 1734
TaqII	GACCGA	2 cut(s)	686, 2569
TatI	WGTACW	4 cut(s)	1794, 1959, 2106, 2322
TauI	GCSGC	6 cut(s)	279, 844, 1057, 1081, 1294, 1364
TfiI	GAWTC	9 cut(s)	495, 766, 910, 949, 1060, 1195, 1681, 2674, 2798
Tru1I	TTAA	5 cut(s)	644, 747, 1500, 2937, 3001
Tru9I	TTAA	5 cut(s)	644, 747, 1500, 2937, 3001
TscAI	CASTG	2 cut(s)	1480, 2634
TseFI	GTSAC	3 cut(s)	1244, 2648, 2955
TseI	GCWGC	6 cut(s)	874, 1051, 1288, 1916, 2353, 2414
Tsp45I	GTSAC	3 cut(s)	1244, 2648, 2955
TspDTI	ATGAA	6 cut(s)	17, 905, 1736, 1897, 2453, 2985
TspGWI	ACGGA	3 cut(s)	214, 797, 2933
TspMI	CCCGGG	1 cut(s)	613
TspRI	CASTG	2 cut(s)	1480, 2634
Tth111I	GACNNNGTC	1 cut(s)	2777
VpaK11BI	GGWCC	7 cut(s)	511, 658, 736, 1525, 2206, 2773, 2827
XagI	CCTNNNNNAGG	1 cut(s)	2066
XapI	RAATTY	2 cut(s)	474, 2714
XceI	RCATGY	4 cut(s)	198, 1298, 1651, 2329
XhoI	CTCGAG	3 cut(s)	506, 653, 1307
XmaI	CCCGGG	1 cut(s)	613
XmiI	GTMKAC	4 cut(s)	228, 525, 712, 2235
XmnI	GAANNNNTTC	3 cut(s)	965, 1440, 1469
Zsp2I	ATGCAT	2 cut(s)	2623, 2980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.