MD15G1283200.v1.1
ERF Family

Ethylene-responsive transcription factor ERF109-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000568
Physical Location & Seq
Reverse (-)
25597390 .. 25598265
876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1283200.v1.1.491

Sequence Viewer

Length: 876 bp
ATGCCCTTCCATGCGAATAGGATACAGAAGGAGCAGGAGCACTGCATCATGGTCTCCGCCCTCAAGCACGTAATCTCCGGTGGGAGCATCAGCGGGCCCACCCCTCAGCACCCGCAGCCAATGCCTGCCGTCTACAATGCCATGTCATCCGTCTCGACGAGCGGCACCCAGTCGGCGGCGGGCCAACCAGCACAACAGGACCAGGGCAGGCTGGCCTTCTTACTAGACGGGGACACGTGTCGGGTGTGCGGGATACCAGGGTGTCTGGGGTGCAACTACTTCCCGCCATCGTTGCCGAATCAAAACAGGAACCAGCAACTGAGCTTGGGAACCGGGTTTGTCGGGATGAATGCGCCAACTACGAGGAAGAGCAAGAACAAGTACAGGGGCGTCAGGCAGAGGCCGTGGGGGAAATGGGCGGCGGAGATTCGAGACCCACGACGGGCGAAGAGGATGTGGCTAGGGACGTTCGAGACGGGGGAGGACGCGGCCAGGGCTTACGACAAGGCCGCCGTCGAGTTCCACGGAAATAAGGCAAAGCTCAATTTCCCATCGGACCCGGGAGGTCACATTGTCACGACTAACGACAGTTCTAGTAGTGGAACTAGTGCTAATGCCAGTATTAATCCAGGATTAATTAATAAGCAAAAGCAAAAGAATATTAGCGAAATTGAGGTCATGGAGAATGAGGAGGAGAAGGTTGATCAGGTCAAACTCAACCAGGCGACTCAGCCGGAGAATATGGAACTGGTGGCGGTGGCGACGGCGAAGAGCAGCGTTGGTCATGAGGAGGATGACCAGTTCTTGTTGTGGGACAATGGCTTGCTCCGAGATGGTGAAGATGATGACTTAATGGCATGGTTGTCCATGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

292

Amino Acids

31.81

Weight (kDa)

6.18

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 127 - 176 3.6e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20350 AT5G67000 AT5G67010
fragaria_vesca FvH4_1g15960
malus_domestica MD02G1171300.v1.1 MD15G1283200.v1.1
prunus_persica Prupe.7G134100_v2.0.a1
pyrus_communis pycom02g13800 pycom15g24630
rosa_chinensis RchiOBHm_Chr1g0349631 RchiOBHm_Chr2g0105221 RchiOBHm_Chr2g0105401 RchiOBHm_Chr2g0105421 RchiOBHm_Chr2g0105461 RchiOBHm_Chr2g0105481 RchiOBHm_Chr2g0105501 RchiOBHm_Chr2g0105521 RchiOBHm_Chr2g0130611
rosa_laevigata RLG00000017410 RLG00000017411 RLG00000017421 RLG00000017424 RLG00000017427 RLG00000017428 RLG00000017430 RLG00000019140 RLG00000019141
rosa_multiflora Rmu_co8006714.1_g000001 Rmu_co8096516.1_g000001 Rmu_co8328685.1_g000001 Rmu_sc0001051.1_g000001 Rmu_sc0001122.1_g000006 Rmu_sc0001407.1_g000003 Rmu_sc0001593.1_g000004 Rmu_sc0004768.1_g000002 Rmu_sc0004768.1_g000004 Rmu_sc0004768.1_g000006 Rmu_sc0005621.1_g000003 Rmu_sc0008529.1_g000003 Rmu_sc0008529.1_g000015 Rmu_sc0017668.1_g000003 Rmu_sc0018542.1_g000001
rosa_roxburghii Rroxscaffold_2G00113840 Rroxscaffold_2G00138130 Rroxscaffold_2G00138170 Rroxscaffold_2G00138190 Rroxscaffold_2G00138250 Rroxscaffold_2G00138360
rosa_rugosa Rorug02G0127100 Rorug02G0127900 Rorug02G0292400 Rorug02G0292600
rosa_samantha Rh1AG222300 Rh2AG177700 Rh2AG178800 Rh2AG179000 Rh2AG179400 Rh2AG179600 Rh2AG179800 Rh2AG343900 Rh2BG185900 Rh2BG187900 Rh2BG188200 Rh2BG188700 Rh2BG352300 Rh2CG182200 Rh2CG183200 Rh2CG183300 Rh2CG183400 Rh2CG183600 Rh2CG183700 Rh2CG183900 Rh2CG331000 Rh2DG183700 Rh2DG184600 Rh2DG184900 Rh2DG185200 Rh2DG185400 Rh2DG185600 Rh2DG370400
rosa_wichuraiana Rw1G018940 Rw2G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 1 cut(s) 132
AccII CGCG 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 489
AcvI CACGTG 1 cut(s) 237
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 2 cut(s) 175, 442
AflIII ACRYGT 2 cut(s) 234, 236
AhlI ACTAGT 1 cut(s) 605
AjnI CCWGG 5 cut(s) 201, 256, 491, 628, 720
AloI GAACNNNNNNTCC 2 cut(s) 785, 817
AluBI AGCT 2 cut(s) 324, 541
AluI AGCT 2 cut(s) 324, 541
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 4 cut(s) 58, 157, 426, 467
AlwNI CAGNNNCTG 1 cut(s) 319
Ama87I CYCGRG 1 cut(s) 559
AoxI GGCC 6 cut(s) 95, 181, 213, 401, 489, 507
ApaI GGGCCC 1 cut(s) 99
ApeKI GCWGC 2 cut(s) 115, 774
ArsI GACNNNNNNTTYG 2 cut(s) 660, 692
AseI ATTAAT 3 cut(s) 624, 635, 639
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 5 cut(s) 95, 96, 181, 199, 556
AsuC2I CCSGG 3 cut(s) 334, 560, 561
AsuHPI GGTGA 1 cut(s) 848
AvaI CYCGRG 1 cut(s) 559
AvaII GGWCC 2 cut(s) 199, 556
BaeGI GKGCMC 1 cut(s) 99
BaeI ACNNNNGTAYC 2 cut(s) 245, 278
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 99
BbrPI CACGTG 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 42
BbvCI CCTCAGC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 127, 786
BccI CCATC 3 cut(s) 295, 559, 827
BceAI ACGGC 4 cut(s) 113, 388, 497, 780
BciT130I CCWGG 5 cut(s) 203, 258, 493, 630, 722
BciVI GTATCC 2 cut(s) 15, 246
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 3 cut(s) 334, 560, 561
BcoDI GTCTC 4 cut(s) 58, 157, 426, 467
BcuI ACTAGT 1 cut(s) 605
BfaI CTAG 5 cut(s) 224, 461, 594, 606, 874
BfuI GTATCC 2 cut(s) 15, 246
BisI GCNGC 7 cut(s) 116, 163, 177, 420, 489, 510, 775
BlsI GCNGC 7 cut(s) 117, 164, 178, 421, 490, 511, 776
Bme1390I CCNGG 8 cut(s) 203, 258, 334, 493, 560, 561, 630, 722
Bme18I GGWCC 2 cut(s) 199, 556
BmeT110I CYCGRG 1 cut(s) 559
BmgT120I GGNCC 5 cut(s) 95, 96, 181, 199, 556
BmiI GGNNCC 5 cut(s) 97, 166, 311, 331, 558
BmrFI CCNGG 8 cut(s) 203, 258, 334, 493, 560, 561, 630, 722
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 2 cut(s) 54, 96
BmuI ACTGGG 1 cut(s) 163
BoxI GACNNNNGTC 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 3 cut(s) 334, 560, 561
BsaAI YACGTR 2 cut(s) 70, 237
BsaHI GRCGYC 1 cut(s) 390
BsaI GGTCTC 2 cut(s) 58, 426
BsaJI CCNNGG 6 cut(s) 202, 257, 404, 492, 523, 559
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 442
Bse1I ACTGG 4 cut(s) 169, 618, 753, 799
BseBI CCWGG 5 cut(s) 203, 258, 493, 630, 722
BseDI CCNNGG 6 cut(s) 202, 257, 404, 492, 523, 559
BseGI GGATG 4 cut(s) 146, 351, 459, 799
BseLI CCNNNNNNNGG 2 cut(s) 175, 442
BseMII CTCAG 3 cut(s) 119, 311, 743
BseNI ACTGG 4 cut(s) 169, 618, 753, 799
BseRI GAGGAG 3 cut(s) 704, 707, 803
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 2 cut(s) 127, 786
Bsh1236I CGCG 1 cut(s) 488
BshFI GGCC 6 cut(s) 97, 183, 215, 403, 491, 509
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 1 cut(s) 559
BsiSI CCGG 4 cut(s) 78, 333, 560, 734
BslFI GGGAC 3 cut(s) 245, 478, 827
BslI CCNNNNNNNGG 2 cut(s) 175, 442
BsmAI GTCTC 4 cut(s) 58, 157, 426, 467
BsmBI CGTCTC 2 cut(s) 157, 467
BsmFI GGGAC 3 cut(s) 245, 478, 827
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 6 cut(s) 97, 183, 215, 403, 491, 509
Bso31I GGTCTC 2 cut(s) 58, 426
BsoBI CYCGRG 1 cut(s) 559
Bsp120I GGGCCC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 42, 99
Bsp143I GATC 1 cut(s) 703
BspANI GGCC 6 cut(s) 97, 183, 215, 403, 491, 509
BspCNI CTCAG 3 cut(s) 118, 312, 742
BspFNI CGCG 1 cut(s) 488
BspHI TCATGA 1 cut(s) 784
BspLI GGNNCC 5 cut(s) 97, 166, 311, 331, 558
BspQI GCTCTTC 2 cut(s) 362, 764
BspT107I GGYRCC 1 cut(s) 164
BspTNI GGTCTC 2 cut(s) 58, 426
BsrBI CCGCTC 1 cut(s) 162
BsrI ACTGG 4 cut(s) 169, 618, 753, 799
BssECI CCNNGG 6 cut(s) 202, 257, 404, 492, 523, 559
BssMI GATC 1 cut(s) 703
BssNI GRCGYC 1 cut(s) 390
Bst2UI CCWGG 5 cut(s) 203, 258, 493, 630, 722
Bst4CI ACNGT 1 cut(s) 590
Bst6I CTCTTC 3 cut(s) 362, 443, 764
BstACI GRCGYC 1 cut(s) 390
BstAPI GCANNNNNTGC 1 cut(s) 121
BstBAI YACGTR 2 cut(s) 70, 237
BstC8I GCNNGC 6 cut(s) 95, 126, 181, 209, 213, 824
BstDEI CTNAG 3 cut(s) 105, 320, 729
BstDSI CCRYGG 2 cut(s) 404, 523
BstF5I GGATG 4 cut(s) 146, 351, 459, 799
BstFNI CGCG 1 cut(s) 488
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 1 cut(s) 706
BstMAI GTCTC 4 cut(s) 58, 157, 426, 467
BstMBI GATC 1 cut(s) 703
BstMWI GCNNNNNNNGC 4 cut(s) 115, 121, 292, 494
BstNI CCWGG 5 cut(s) 203, 258, 493, 630, 722
BstPAI GACNNNNGTC 1 cut(s) 237
BstSCI CCNGG 8 cut(s) 201, 256, 332, 491, 558, 559, 628, 720
BstSLI GKGCMC 1 cut(s) 99
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 2 cut(s) 127, 786
BsuI GTATCC 2 cut(s) 15, 246
BsuRI GGCC 6 cut(s) 97, 183, 215, 403, 491, 509
BtgI CCRYGG 2 cut(s) 404, 523
BtsCI GGATG 4 cut(s) 146, 351, 459, 799
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 6 cut(s) 95, 126, 181, 209, 213, 824
CaiI CAGNNNCTG 1 cut(s) 319
CciI TCATGA 1 cut(s) 784
CfoI GCGC 1 cut(s) 355
Cfr13I GGNCC 5 cut(s) 95, 96, 181, 199, 556
Cfr9I CCCGGG 1 cut(s) 559
CseI GACGC 2 cut(s) 379, 494
Csp6I GTAC 1 cut(s) 382
CviAII CATG 7 cut(s) 11, 49, 142, 679, 785, 858, 868
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 3 cut(s) 105, 320, 729
DpnI GATC 1 cut(s) 705
DpnII GATC 1 cut(s) 703
EaeI YGGCCR 1 cut(s) 489
Eam1104I CTCTTC 3 cut(s) 362, 443, 764
EarI CTCTTC 3 cut(s) 362, 443, 764
EciI GGCGGA 2 cut(s) 46, 437
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 2 cut(s) 58, 426
Eco47I GGWCC 2 cut(s) 199, 556
Eco72I CACGTG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 559
EcoRII CCWGG 5 cut(s) 201, 256, 491, 628, 720
EcoT38I GRGCYC 1 cut(s) 99
Esp3I CGTCTC 2 cut(s) 157, 467
FaeI CATG 7 cut(s) 14, 52, 145, 682, 788, 861, 871
FaiI YATR 8 cut(s) 12, 50, 143, 680, 743, 786, 859, 869
FaqI GGGAC 3 cut(s) 245, 478, 827
FatI CATG 7 cut(s) 10, 48, 141, 678, 784, 857, 867
FauI CCCGC 5 cut(s) 86, 120, 172, 242, 291
FbaI TGATCA 1 cut(s) 703
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 7 cut(s) 116, 163, 177, 420, 489, 510, 775
FokI GGATG 4 cut(s) 133, 358, 466, 806
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 7 cut(s) 116, 163, 177, 420, 489, 510, 775
FspBI CTAG 5 cut(s) 224, 461, 594, 606, 874
GlaI GCGC 1 cut(s) 354
GluI GCNGC 7 cut(s) 116, 163, 177, 420, 489, 510, 775
HaeIII GGCC 6 cut(s) 97, 183, 215, 403, 491, 509
HapII CCGG 4 cut(s) 78, 333, 560, 734
HgaI GACGC 2 cut(s) 379, 494
HhaI GCGC 1 cut(s) 355
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 7 cut(s) 14, 52, 145, 682, 788, 861, 871
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HinfI GANTC 3 cut(s) 298, 427, 727
HpaII CCGG 4 cut(s) 78, 333, 560, 734
HphI GGTGA 1 cut(s) 848
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 2 cut(s) 556, 830
Hpy188III TCNNGA 6 cut(s) 154, 343, 431, 472, 577, 785
Hpy8I GTNNAC 1 cut(s) 133
Hpy99I CGWCG 4 cut(s) 160, 444, 518, 766
HpyAV CCTTC 4 cut(s) 16, 22, 226, 691
HpyCH4III ACNGT 1 cut(s) 590
HpyCH4IV ACGT 3 cut(s) 69, 236, 467
HpyCH4V TGCA 2 cut(s) 45, 273
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 121, 292, 494
HpyF3I CTNAG 3 cut(s) 105, 320, 729
HpySE526I ACGT 3 cut(s) 69, 236, 467
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 7 cut(s) 14, 52, 145, 682, 788, 861, 871
HspAI GCGC 1 cut(s) 353
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 1 cut(s) 703
LguI GCTCTTC 2 cut(s) 362, 764
LmnI GCTCC 4 cut(s) 31, 37, 84, 831
Lsp1109I GCAGC 2 cut(s) 127, 786
LweI GCATC 2 cut(s) 54, 96
MaeI CTAG 5 cut(s) 224, 461, 594, 606, 874
MaeII ACGT 3 cut(s) 69, 236, 467
MaeIII GTNAC 2 cut(s) 566, 574
MalI GATC 1 cut(s) 705
MbiI CCGCTC 1 cut(s) 162
MboI GATC 1 cut(s) 703
MboII GAAGA 4 cut(s) 379, 460, 781, 851
MhlI GDGCHC 2 cut(s) 42, 99
MluCI AATT 3 cut(s) 544, 636, 669
MlyI GAGTC 1 cut(s) 721
MseI TTAA 4 cut(s) 624, 635, 639, 851
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 4 cut(s) 78, 333, 560, 734
MspR9I CCNGG 8 cut(s) 203, 258, 334, 493, 560, 561, 630, 722
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 5 cut(s) 203, 258, 493, 630, 722
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 4 cut(s) 115, 121, 292, 494
NciI CCSGG 3 cut(s) 334, 560, 561
NdeII GATC 1 cut(s) 703
NlaIII CATG 7 cut(s) 14, 52, 145, 682, 788, 861, 871
NlaIV GGNNCC 5 cut(s) 97, 166, 311, 331, 558
NmuCI GTSAC 2 cut(s) 566, 574
PacI TTAATTAA 1 cut(s) 639
PagI TCATGA 1 cut(s) 784
PciSI GCTCTTC 2 cut(s) 362, 764
PcsI WCGNNNNNNNCGW 1 cut(s) 436
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 2 cut(s) 298, 427
PfoI TCCNGGA 1 cut(s) 628
PkrI GCNGC 7 cut(s) 117, 164, 178, 421, 490, 511, 776
PleI GAGTC 1 cut(s) 721
PmaCI CACGTG 1 cut(s) 237
PmlI CACGTG 1 cut(s) 237
PpsI GAGTC 1 cut(s) 721
Ppu21I YACGTR 2 cut(s) 70, 237
PshAI GACNNNNGTC 1 cut(s) 237
PshBI ATTAAT 3 cut(s) 624, 635, 639
Psp6I CCWGG 5 cut(s) 201, 256, 491, 628, 720
PspCI CACGTG 1 cut(s) 237
PspGI CCWGG 5 cut(s) 201, 256, 491, 628, 720
PspN4I GGNNCC 5 cut(s) 97, 166, 311, 331, 558
PspOMI GGGCCC 1 cut(s) 95
PspPI GGNCC 5 cut(s) 95, 96, 181, 199, 556
PstNI CAGNNNCTG 1 cut(s) 319
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
SapI GCTCTTC 2 cut(s) 362, 764
SaqAI TTAA 4 cut(s) 624, 635, 639, 851
SatI GCNGC 7 cut(s) 116, 163, 177, 420, 489, 510, 775
Sau3AI GATC 1 cut(s) 703
Sau96I GGNCC 5 cut(s) 95, 96, 181, 199, 556
SchI GAGTC 1 cut(s) 721
ScrFI CCNGG 8 cut(s) 203, 258, 334, 493, 560, 561, 630, 722
SduI GDGCHC 2 cut(s) 42, 99
SetI ASST 9 cut(s) 72, 239, 326, 470, 543, 568, 678, 702, 711
SfaNI GCATC 2 cut(s) 54, 96
SinI GGWCC 2 cut(s) 199, 556
SmaI CCCGGG 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SpeI ACTAGT 1 cut(s) 605
Sse9I AATT 3 cut(s) 544, 636, 669
SspI AATATT 1 cut(s) 661
SspMI CTAG 5 cut(s) 224, 461, 594, 606, 874
StyD4I CCNGG 8 cut(s) 201, 256, 332, 491, 558, 559, 628, 720
TaaI ACNGT 1 cut(s) 590
TaiI ACGT 3 cut(s) 72, 239, 470
TaqI TCGA 4 cut(s) 155, 430, 471, 516
TasI AATT 3 cut(s) 544, 636, 669
TatI WGTACW 1 cut(s) 381
TauI GCSGC 5 cut(s) 165, 179, 422, 491, 512
TfiI GAWTC 2 cut(s) 298, 427
Tru1I TTAA 4 cut(s) 624, 635, 639, 851
Tru9I TTAA 4 cut(s) 624, 635, 639, 851
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 2 cut(s) 566, 574
TseI GCWGC 2 cut(s) 115, 774
Tsp45I GTSAC 2 cut(s) 566, 574
TspDTI ATGAA 1 cut(s) 362
TspGWI ACGGA 2 cut(s) 139, 540
TspMI CCCGGG 1 cut(s) 559
TspRI CASTG 1 cut(s) 47
VpaK11BI GGWCC 2 cut(s) 199, 556
VspI ATTAAT 3 cut(s) 624, 635, 639
XmaI CCCGGG 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 5 cut(s) 224, 461, 594, 606, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.