pycom15g24630
ERF Family

Ethylene-responsive transcription factor ERF109-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000568
Physical Location & Seq
Reverse (-)
18851857 .. 18852651
795 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g24630.1

Sequence Viewer

Length: 795 bp
ATGCCCTTCCATGCGAATCGGATACAGCAGGAGCAGGAACACTGCATCATGGTCTCCGCCCTCAAGCACGTAATCTCCGGTGGAAGCATCAGTGGGCTCACACCTCAGCCAATGCCGGCGGTCTACAATGCCACGTCATCCGTCTCGACGAGCGGCACCCAGTTGGCAGCGGGCCAACCAGCACAACAGGACAACTACTTCTCGCCATCGTTGCCGAATCAAAACAGGAACCAGCAACTGAGTTTGGGAACCGGGTTTGTCGGGATTAATGCGCCAACTACGAGGAAGAGCAAGAACAAGTACAGGGGCGTCAGGCAGAGGCCGTGGGGGAAATGGGCGGCGGAGATTCGAGACCCACGACGGGCGGCGAGGGTGTGGCTAGGGACGTTCGAGACGGCGGAGGACGCGGCCAGGGCTTACGACAAGGCCGCCGTGGAGTTCCGCGGAAATAAGGCAAAGCTCAATTTCCCATCGGACCCGGGCGGTCACATTGTCACGACTAACGACAGTTCTAGTAGTGGAACTAGTGCTAATGCCAGTATTAATCCAGGATTAATTAATAAGCAAAAGCAAAAGAATATTAGCGAAATTGGGGTCATGGAGAAGGAGGAGGAGAAGGTTGATCAGGTCAAACTCAACCAGGCGACGCAGCCGGAGAATATGCAACTGGGGGTGGTGGCGACGGCGGAGAGCAGCGTTGGTCATGAGGAGGATGACCAGTTCTTGTTGTGGGACAATGGCTTGCTCCGAGATGGTGAAGATGATGACTTAATGGCATGGTTATCCACGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

265

Amino Acids

28.82

Weight (kDa)

5.99

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 100 - 149 3e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20350 AT5G67000 AT5G67010
fragaria_vesca FvH4_1g15960
malus_domestica MD02G1171300.v1.1 MD15G1283200.v1.1
prunus_persica Prupe.7G134100_v2.0.a1
pyrus_communis pycom02g13800 pycom15g24630
rosa_chinensis RchiOBHm_Chr1g0349631 RchiOBHm_Chr2g0105221 RchiOBHm_Chr2g0105401 RchiOBHm_Chr2g0105421 RchiOBHm_Chr2g0105461 RchiOBHm_Chr2g0105481 RchiOBHm_Chr2g0105501 RchiOBHm_Chr2g0105521 RchiOBHm_Chr2g0130611
rosa_laevigata RLG00000017410 RLG00000017411 RLG00000017421 RLG00000017424 RLG00000017427 RLG00000017428 RLG00000017430 RLG00000019140 RLG00000019141
rosa_multiflora Rmu_co8006714.1_g000001 Rmu_co8096516.1_g000001 Rmu_co8328685.1_g000001 Rmu_sc0001051.1_g000001 Rmu_sc0001122.1_g000006 Rmu_sc0001407.1_g000003 Rmu_sc0001593.1_g000004 Rmu_sc0004768.1_g000002 Rmu_sc0004768.1_g000004 Rmu_sc0004768.1_g000006 Rmu_sc0005621.1_g000003 Rmu_sc0008529.1_g000003 Rmu_sc0008529.1_g000015 Rmu_sc0017668.1_g000003 Rmu_sc0018542.1_g000001
rosa_roxburghii Rroxscaffold_2G00113840 Rroxscaffold_2G00138130 Rroxscaffold_2G00138170 Rroxscaffold_2G00138190 Rroxscaffold_2G00138250 Rroxscaffold_2G00138360
rosa_rugosa Rorug02G0127100 Rorug02G0127900 Rorug02G0292400 Rorug02G0292600
rosa_samantha Rh1AG222300 Rh2AG177700 Rh2AG178800 Rh2AG179000 Rh2AG179400 Rh2AG179600 Rh2AG179800 Rh2AG343900 Rh2BG185900 Rh2BG187900 Rh2BG188200 Rh2BG188700 Rh2BG352300 Rh2CG182200 Rh2CG183200 Rh2CG183300 Rh2CG183400 Rh2CG183600 Rh2CG183700 Rh2CG183900 Rh2CG331000 Rh2DG183700 Rh2DG184600 Rh2DG184900 Rh2DG185200 Rh2DG185400 Rh2DG185600 Rh2DG370400
rosa_wichuraiana Rw1G018940 Rw2G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 153
AccI GTMKAC 1 cut(s) 123
AccII CGCG 2 cut(s) 407, 444
AcoI YGGCCR 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 1 cut(s) 361
AhlI ACTAGT 1 cut(s) 524
AjiI CACGTC 1 cut(s) 135
AjnI CCWGG 3 cut(s) 410, 547, 639
AloI GAACNNNNNNTCC 2 cut(s) 704, 736
AluBI AGCT 1 cut(s) 460
AluI AGCT 1 cut(s) 460
Alw26I GTCTC 4 cut(s) 58, 148, 345, 386
AlwNI CAGNNNCTG 1 cut(s) 238
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 4 cut(s) 172, 320, 408, 426
ApeKI GCWGC 3 cut(s) 167, 649, 693
ArsI GACNNNNNNTTYG 2 cut(s) 579, 611
AseI ATTAAT 4 cut(s) 267, 543, 554, 558
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 2 cut(s) 172, 475
AsuC2I CCSGG 3 cut(s) 253, 479, 480
AsuHPI GGTGA 1 cut(s) 767
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 105
BbvI GCAGC 3 cut(s) 179, 661, 705
BccI CCATC 3 cut(s) 214, 478, 746
BceAI ACGGC 4 cut(s) 307, 411, 416, 699
BciT130I CCWGG 3 cut(s) 412, 549, 641
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 622
BcnI CCSGG 3 cut(s) 253, 479, 480
BcoDI GTCTC 4 cut(s) 58, 148, 345, 386
BcuI ACTAGT 1 cut(s) 524
BfaI CTAG 4 cut(s) 380, 513, 525, 793
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 8 cut(s) 154, 168, 339, 366, 408, 429, 650, 694
BlsI GCNGC 8 cut(s) 155, 169, 340, 367, 409, 430, 651, 695
Bme1390I CCNGG 6 cut(s) 253, 412, 479, 480, 549, 641
Bme18I GGWCC 1 cut(s) 475
BmeT110I CYCGRG 1 cut(s) 478
BmgBI CACGTC 1 cut(s) 135
BmgT120I GGNCC 2 cut(s) 172, 475
BmiI GGNNCC 4 cut(s) 157, 230, 250, 477
BmrFI CCNGG 6 cut(s) 253, 412, 479, 480, 549, 641
BmrI ACTGGG 2 cut(s) 154, 677
BmsI GCATC 2 cut(s) 54, 96
BmuI ACTGGG 2 cut(s) 154, 677
Bpu10I CCTNAGC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 3 cut(s) 253, 479, 480
BsaAI YACGTR 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 2 cut(s) 58, 345
BsaJI CCNNGG 5 cut(s) 323, 411, 432, 442, 478
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 4 cut(s) 160, 537, 672, 718
BseBI CCWGG 3 cut(s) 412, 549, 641
BseDI CCNNGG 5 cut(s) 323, 411, 432, 442, 478
BseGI GGATG 2 cut(s) 137, 718
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMII CTCAG 2 cut(s) 119, 230
BseNI ACTGG 4 cut(s) 160, 537, 672, 718
BseRI GAGGAG 3 cut(s) 623, 626, 722
BseXI GCAGC 3 cut(s) 179, 661, 705
Bsh1236I CGCG 2 cut(s) 407, 444
BshFI GGCC 4 cut(s) 174, 322, 410, 428
BshNI GGYRCC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 5 cut(s) 78, 116, 252, 479, 653
BslFI GGGAC 2 cut(s) 397, 746
BslI CCNNNNNNNGG 1 cut(s) 361
BsmAI GTCTC 4 cut(s) 58, 148, 345, 386
BsmBI CGTCTC 2 cut(s) 148, 386
BsmFI GGGAC 2 cut(s) 397, 746
BsnI GGCC 4 cut(s) 174, 322, 410, 428
Bso31I GGTCTC 2 cut(s) 58, 345
BsoBI CYCGRG 1 cut(s) 478
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 1 cut(s) 622
BspANI GGCC 4 cut(s) 174, 322, 410, 428
BspCNI CTCAG 2 cut(s) 118, 231
BspFNI CGCG 2 cut(s) 407, 444
BspHI TCATGA 1 cut(s) 703
BspLI GGNNCC 4 cut(s) 157, 230, 250, 477
BspQI GCTCTTC 1 cut(s) 281
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 2 cut(s) 58, 345
BsrBI CCGCTC 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 4 cut(s) 160, 537, 672, 718
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 5 cut(s) 323, 411, 432, 442, 478
BssMI GATC 1 cut(s) 622
BssNI GRCGYC 1 cut(s) 309
Bst2UI CCWGG 3 cut(s) 412, 549, 641
Bst4CI ACNGT 1 cut(s) 509
Bst6I CTCTTC 1 cut(s) 281
BstACI GRCGYC 1 cut(s) 309
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 3 cut(s) 117, 172, 743
BstDEI CTNAG 2 cut(s) 105, 239
BstDSI CCRYGG 3 cut(s) 323, 432, 442
BstF5I GGATG 2 cut(s) 137, 718
BstFNI CGCG 2 cut(s) 407, 444
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 1 cut(s) 625
BstMAI GTCTC 4 cut(s) 58, 148, 345, 386
BstMBI GATC 1 cut(s) 622
BstMWI GCNNNNNNNGC 3 cut(s) 211, 404, 413
BstNI CCWGG 3 cut(s) 412, 549, 641
BstSCI CCNGG 6 cut(s) 251, 410, 477, 478, 547, 639
BstUI CGCG 2 cut(s) 407, 444
BstV1I GCAGC 3 cut(s) 179, 661, 705
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 4 cut(s) 174, 322, 410, 428
BtgI CCRYGG 3 cut(s) 323, 432, 442
BtrI CACGTC 1 cut(s) 135
BtsCI GGATG 2 cut(s) 137, 718
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 2 cut(s) 40, 97
Cac8I GCNNGC 3 cut(s) 117, 172, 743
CaiI CAGNNNCTG 1 cut(s) 238
CciI TCATGA 1 cut(s) 703
CfoI GCGC 1 cut(s) 274
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 2 cut(s) 172, 475
Cfr42I CCGCGG 1 cut(s) 445
Cfr9I CCCGGG 1 cut(s) 478
CseI GACGC 3 cut(s) 298, 413, 655
Csp6I GTAC 1 cut(s) 301
CviAII CATG 5 cut(s) 11, 49, 598, 704, 777
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 2 cut(s) 105, 239
DpnI GATC 1 cut(s) 624
DpnII GATC 1 cut(s) 622
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
EciI GGCGGA 4 cut(s) 46, 356, 413, 701
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 2 cut(s) 58, 345
Eco47I GGWCC 1 cut(s) 475
Eco88I CYCGRG 1 cut(s) 478
EcoRII CCWGG 3 cut(s) 410, 547, 639
EcoT38I GRGCYC 1 cut(s) 99
Esp3I CGTCTC 2 cut(s) 148, 386
FaeI CATG 5 cut(s) 14, 52, 601, 707, 780
FaiI YATR 6 cut(s) 12, 50, 599, 662, 705, 778
FaqI GGGAC 2 cut(s) 397, 746
FatI CATG 5 cut(s) 10, 48, 597, 703, 776
FauI CCCGC 1 cut(s) 163
FbaI TGATCA 1 cut(s) 622
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 8 cut(s) 154, 168, 339, 366, 408, 429, 650, 694
FokI GGATG 2 cut(s) 124, 725
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 8 cut(s) 154, 168, 339, 366, 408, 429, 650, 694
FspBI CTAG 4 cut(s) 380, 513, 525, 793
GlaI GCGC 1 cut(s) 273
GluI GCNGC 8 cut(s) 154, 168, 339, 366, 408, 429, 650, 694
HaeIII GGCC 4 cut(s) 174, 322, 410, 428
HapII CCGG 5 cut(s) 78, 116, 252, 479, 653
HgaI GACGC 3 cut(s) 298, 413, 655
HhaI GCGC 1 cut(s) 274
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 5 cut(s) 14, 52, 601, 707, 780
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HinfI GANTC 3 cut(s) 16, 217, 346
HpaII CCGG 5 cut(s) 78, 116, 252, 479, 653
HphI GGTGA 1 cut(s) 767
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 3 cut(s) 21, 475, 749
Hpy188III TCNNGA 6 cut(s) 145, 262, 350, 391, 496, 704
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 4 cut(s) 151, 363, 649, 685
HpyAV CCTTC 3 cut(s) 16, 598, 610
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4IV ACGT 3 cut(s) 69, 134, 386
HpyCH4V TGCA 2 cut(s) 45, 664
HpyF10VI GCNNNNNNNGC 3 cut(s) 211, 404, 413
HpyF3I CTNAG 2 cut(s) 105, 239
HpySE526I ACGT 3 cut(s) 69, 134, 386
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 5 cut(s) 14, 52, 601, 707, 780
HspAI GCGC 1 cut(s) 272
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 622
KspI CCGCGG 1 cut(s) 445
Kzo9I GATC 1 cut(s) 622
LguI GCTCTTC 1 cut(s) 281
LmnI GCTCC 2 cut(s) 31, 750
Lsp1109I GCAGC 3 cut(s) 179, 661, 705
LweI GCATC 2 cut(s) 54, 96
MaeI CTAG 4 cut(s) 380, 513, 525, 793
MaeII ACGT 3 cut(s) 69, 134, 386
MaeIII GTNAC 2 cut(s) 485, 493
MalI GATC 1 cut(s) 624
MbiI CCGCTC 1 cut(s) 153
MboI GATC 1 cut(s) 622
MboII GAAGA 2 cut(s) 298, 770
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 3 cut(s) 463, 555, 588
MroNI GCCGGC 1 cut(s) 115
MseI TTAA 5 cut(s) 267, 543, 554, 558, 770
MspA1I CMGCKG 2 cut(s) 170, 444
MspI CCGG 5 cut(s) 78, 116, 252, 479, 653
MspR9I CCNGG 6 cut(s) 253, 412, 479, 480, 549, 641
MvaI CCWGG 3 cut(s) 412, 549, 641
MvnI CGCG 2 cut(s) 407, 444
MwoI GCNNNNNNNGC 3 cut(s) 211, 404, 413
NaeI GCCGGC 1 cut(s) 117
NciI CCSGG 3 cut(s) 253, 479, 480
NdeII GATC 1 cut(s) 622
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 5 cut(s) 14, 52, 601, 707, 780
NlaIV GGNNCC 4 cut(s) 157, 230, 250, 477
NmuCI GTSAC 2 cut(s) 485, 493
PacI TTAATTAA 1 cut(s) 558
PagI TCATGA 1 cut(s) 703
PciSI GCTCTTC 1 cut(s) 281
PcsI WCGNNNNNNNCGW 1 cut(s) 355
PdiI GCCGGC 1 cut(s) 117
PfeI GAWTC 3 cut(s) 16, 217, 346
PfoI TCCNGGA 1 cut(s) 547
PkrI GCNGC 8 cut(s) 155, 169, 340, 367, 409, 430, 651, 695
Ppu21I YACGTR 1 cut(s) 70
PshBI ATTAAT 4 cut(s) 267, 543, 554, 558
Psp6I CCWGG 3 cut(s) 410, 547, 639
PspGI CCWGG 3 cut(s) 410, 547, 639
PspN4I GGNNCC 4 cut(s) 157, 230, 250, 477
PspPI GGNCC 2 cut(s) 172, 475
PstNI CAGNNNCTG 1 cut(s) 238
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
SacII CCGCGG 1 cut(s) 445
SapI GCTCTTC 1 cut(s) 281
SaqAI TTAA 5 cut(s) 267, 543, 554, 558, 770
SatI GCNGC 8 cut(s) 154, 168, 339, 366, 408, 429, 650, 694
Sau3AI GATC 1 cut(s) 622
Sau96I GGNCC 2 cut(s) 172, 475
ScrFI CCNGG 6 cut(s) 253, 412, 479, 480, 549, 641
SduI GDGCHC 1 cut(s) 99
SetI ASST 7 cut(s) 72, 106, 137, 389, 462, 621, 630
SfaNI GCATC 2 cut(s) 54, 96
Sfr303I CCGCGG 1 cut(s) 445
SgrBI CCGCGG 1 cut(s) 445
SinI GGWCC 1 cut(s) 475
SmaI CCCGGG 1 cut(s) 480
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SpeI ACTAGT 1 cut(s) 524
Sse9I AATT 3 cut(s) 463, 555, 588
SspI AATATT 1 cut(s) 580
SspMI CTAG 4 cut(s) 380, 513, 525, 793
StyD4I CCNGG 6 cut(s) 251, 410, 477, 478, 547, 639
TaaI ACNGT 1 cut(s) 509
TaiI ACGT 3 cut(s) 72, 137, 389
TaqI TCGA 3 cut(s) 146, 349, 390
TasI AATT 3 cut(s) 463, 555, 588
TatI WGTACW 1 cut(s) 300
TauI GCSGC 5 cut(s) 156, 341, 368, 410, 431
TfiI GAWTC 3 cut(s) 16, 217, 346
Tru1I TTAA 5 cut(s) 267, 543, 554, 558, 770
Tru9I TTAA 5 cut(s) 267, 543, 554, 558, 770
TscAI CASTG 2 cut(s) 47, 97
TseFI GTSAC 2 cut(s) 485, 493
TseI GCWGC 3 cut(s) 167, 649, 693
Tsp45I GTSAC 2 cut(s) 485, 493
TspGWI ACGGA 1 cut(s) 130
TspMI CCCGGG 1 cut(s) 478
TspRI CASTG 2 cut(s) 47, 97
VpaK11BI GGWCC 1 cut(s) 475
VspI ATTAAT 4 cut(s) 267, 543, 554, 558
XmaI CCCGGG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 4 cut(s) 380, 513, 525, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.