pycom14g12150

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000644
Physical Location & Seq
Forward (+)
15197059 .. 15201971
4913 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2559 bp
ATGGCGCAAGTTAGCCAGCTTCCCTGCGACGGAGACGGCGTCTGCATGCTCTGCAAGGTGACTCCGACTGACGCCGAGAAGCTCACCTGCAAGACCTGCGTGACCCCCTGGCACGCTGCCTGCCTCTTGGCCAAGCCCGAGTCCCTGGCCGCCACTCTCCAGTGGGAGTGCCCCGACTGCACATCAATCTCCGGCGACGCTCCGGCGCACGTCCCCGCCGTGAACGCCGGCGTGGACGGCGGTCTGGTGGCGGCGATCCGGGAGATCGAGAACGACGCGACCCTGACGGAGCAGGAGAAGGCGAGGAGGAGGCAGGAGCTGATGAGCGGGAAGGCGACGGTGGAGGAGGAGAAGAAGGGGGCGAAGGACGTCTTGGATGTGCTCGGTGGGATTTTCAACTGCGTCTTCTGTATGCAGTTACCGGATAGGCCTGTTACGACACCATGTGGACACAATTTCTGCCTGAAGTGCTTCCAGAAATGGGTTGGACAAGGGAAGCGTATTTGTGGAAAATGCCGTGGTCAAATTCCTCCCAAGATGGCAAGTAACCCCCGAATCAACTCTTCACTTGTATCTTGCATACGTACGGCTAAGTTGTCAAAAGCAAATGCAAACGGGGGGCCTGCTCGGGTTTATCATTATGTACAAAATCAAGACAGGCCGGACAAGGCATTCACTACAGAACGTGCACAGAAGACTGGAAAAGCTAATGCTTCAAGTGGCAAGATATTTGTCACGATTCCAGCAGATCACTTTGGACCCATTCCTGCTGAAAATGATCCAGAGAGAAATCAAGGTGTTCTGGTTGGTGAGTGCTGGGAGGATAGGCTTGAATGTAGACAATGGGGTGCACATTTTCCCCATGTTGCTGGTATAGCTGGGCAGTCTGATCATGGTGCACAGTCAGTTGTGCTTTCAGGAGGCTACCTTGATGATGAGGATCATGGAGAGTGGTTCTTATATACTGGAAGTGGTGGAAGGGATTTGAGCGGTAATAAGCGAACAAACAAGGAGCAGTCTTTTGACCAGACATTTGACAAATATAACAAGGCTCTCCGACTCAGTTGCCTGAAAGGTTATCCTGTGCGGGTGGTAAGGTCTCACAAGGAGAAGCGTTCCTCATATGCCCCTGCTGATAAAGGTGTTCGGTATGATGGATGCTACAGAATAGAAAAATGTTGGCTTAAGGTTGGAGTCCAGGGGTTCAAAGTCTGCCGGTATCTTTTTGTCAGATGTGATAATGAACCAGCGCCATGGACGAGTGATGAGCATGGGGATCGACCTAGACCTTTACCTGATATTCCTGAGCTGAAGGCAGCAAAAGATATAACAGTGAGGAAAGAAAGCCCATCTTGGGACTTTGATGAAAAAGATGGTCGCTGGACATGGATGAAGCAACCACCTCCCAGCAAAAAACCTATTCTAGCATTCTGTCCTGAAGCTCAAAGGAAGACAACGAAAGCAATCAAGAAAGCACAAAAAATGACTGTACGACAGAAGCTCTTGAAAGAATTCAGCTGCCAAATTTGTCGGGAGGTGATGACTGCACCAGTAACCACACCTTGTGTTCATCACTTTTGCAAGTCATGCTTAGAAGGTGCTTTTGTTGGCAAGAGTTTTGTGACAGAAAGAAGCAGAGGTGGACGGTCTCTCCGAGCCCAAAAGAATGTGATGAAATGCCCTGCCTGCCCGATGGATATCTCTGATTTTCTGAAGAATCCTCAGGTGAATACAGAGATAAAGGATATGATCGAGTCACTAAAAGCTCAGAATGAAAAGGAAGAGAAGGTGGATCCAACTGAGGAGTCAAGTGATGAAGAGGATGAGGATCCAAATGAGGTGTTAAGTGAAGATGATTCTGACAATGAAACTGCTGATCCTGTGTCTGACGAAGCAAGAGTGAATGGCAACGGGCAGACTTCACGAACAAAGTCGCCCACAAGGAGTCCCAAACGTCTGAAGGTTGATGCTGGTAATTCCTCATCAAAGACTCATGATGATGGGGAGGCAAAGGAAATTTTTGGTGCCCTGACTGATGGTTCGAAGGGAGATCCTAAAGAGAGCAAATCTGATGCTCCTGTTGCAAAGCATGGGAGGAAACCTGCTGCTCAGGGCGGTGCTGGAGCAAAGACCCGAGGAAGAAAGAGGACTAAGGAGGAAGCACCGGGTGATGAAGAAGGAAAATCTTCACCATCAAGTTCTCTACAGGTGCAATCTGATGAGGATCCAAAAGAGAGCGAGAAGCCAGCTGCAAAGTGTGGGAGGAAGCCTGCTAGTCAAGATGGTACTGGAGCGAAGACCAGAGGAAGAAAGAAGGCTCAGGAGAAAGAAGCATCGGGAAATGAAGAAGGAAATGGCTCACAGTCAAGTCCTCAGCAGGTGCAATCTGTTGAGGATCCAAAAGAGAACAAAAAGCCAGCTGCAAAGCGTGGGAGGAAGGCTGCTGGCGAGGGTGGTGCTGGAGCAAAGAAGGCTCAGGAGGAAGAAGCAATGGATGTTGATGATGGAAATGTTTCACCATCAAGCCCTCTGCACGTGAAAGCCGATGATTTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

853

Amino Acids

92.99

Weight (kDa)

8.14

Isoelectric Point (pI)

52.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 133 - 176 3.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 133 - 173 2.3e-06 Ring finger domain
zf-C3HC4 PF00097 134 - 172 2.5e-06 Zinc finger, C3HC4 type (RING finger)
SAD_SRA PF02182 264 - 414 1.5e-48 SAD/SRA domain
zf-RING_UBOX PF13445 507 - 538 8e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 507 - 532 8.8e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 95, 2368
AatII GACGTC 1 cut(s) 372
Acc36I ACCTGC 4 cut(s) 95, 104, 2110, 2368
AccB1I GGYRCC 1 cut(s) 2024
AccBSI CCGCTC 2 cut(s) 327, 990
AccI GTMKAC 1 cut(s) 838
AccII CGCG 1 cut(s) 278
AciI CCGC 8 cut(s) 150, 216, 240, 251, 327, 990, 1087, 2115
AcoI YGGCCR 2 cut(s) 129, 147
AcsI RAATTY 4 cut(s) 525, 1511, 1524, 2016
AcuI CTGAAG 5 cut(s) 485, 1331, 1458, 1733, 1979
AcvI CACGTG 1 cut(s) 2536
AcyI GRCGYC 3 cut(s) 39, 72, 369
AdeI CACNNNGTG 3 cut(s) 446, 1565, 2168
AfaI GTAC 4 cut(s) 586, 645, 1491, 2287
AfiI CCNNNNNNNGG 3 cut(s) 29, 481, 1354
AflII CTTAAG 1 cut(s) 1184
AgsI TTSAA 5 cut(s) 397, 717, 833, 1207, 1507
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 3 cut(s) 107, 144, 1197
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
Alw21I GWGCWC 4 cut(s) 384, 691, 853, 901
Alw26I GTCTC 3 cut(s) 27, 1104, 1653
Alw44I GTGCAC 3 cut(s) 687, 849, 897
AlwNI CAGNNNCTG 1 cut(s) 319
Ama87I CYCGRG 3 cut(s) 137, 627, 2133
AoxI GGCC 5 cut(s) 129, 147, 428, 620, 659
ApaLI GTGCAC 3 cut(s) 687, 849, 897
ApeKI GCWGC 7 cut(s) 116, 1316, 1518, 2105, 2249, 2420, 2441
ApoI RAATTY 4 cut(s) 525, 1511, 1524, 2016
ArsI GACNNNNNNTTYG 4 cut(s) 1016, 1048, 2023, 2055
Asp700I GAANNNNTTC 4 cut(s) 470, 1511, 2185, 2512
AspLEI GCGC 3 cut(s) 7, 208, 1252
AspS9I GGNCC 2 cut(s) 620, 758
AsuC2I CCSGG 2 cut(s) 260, 2166
AsuHPI GGTGA 8 cut(s) 70, 76, 821, 1549, 1738, 2180, 2181, 2508
AsuII TTCGAA 1 cut(s) 2042
AvaI CYCGRG 3 cut(s) 137, 627, 2133
AvaII GGWCC 1 cut(s) 758
AxyI CCTNAGG 1 cut(s) 1722
BaeGI GKGCMC 5 cut(s) 173, 691, 853, 901, 2029
BalI TGGCCA 1 cut(s) 131
BamHI GGATCC 4 cut(s) 1792, 1828, 2224, 2395
BanI GGYRCC 1 cut(s) 2024
BanII GRGCYC 1 cut(s) 1660
BbrPI CACGTG 1 cut(s) 2536
BbsI GAAGAC 4 cut(s) 397, 701, 1457, 2303
Bbv12I GWGCWC 4 cut(s) 384, 691, 853, 901
BbvCI CCTCAGC 1 cut(s) 2373
BbvI GCAGC 7 cut(s) 103, 1328, 1505, 2092, 2236, 2407, 2428
BceAI ACGGC 5 cut(s) 52, 203, 253, 501, 603
BcgI CGANNNNNNTGC 6 cut(s) 1047, 1081, 1259, 1293, 2438, 2472
BciT130I CCWGG 3 cut(s) 109, 146, 1199
BclI TGATCA 1 cut(s) 889
BcnI CCSGG 2 cut(s) 260, 2166
BcoDI GTCTC 3 cut(s) 27, 1104, 1653
BfaI CTAG 3 cut(s) 1284, 1424, 2274
BfmI CTRYAG 3 cut(s) 678, 1162, 2204
BfoI RGCGCY 1 cut(s) 1253
BfrI CTTAAG 1 cut(s) 1184
BfuAI ACCTGC 4 cut(s) 95, 104, 2110, 2368
BisI GCNGC 9 cut(s) 117, 150, 252, 1317, 1519, 2106, 2250, 2421, 2442
BlsI GCNGC 9 cut(s) 118, 151, 253, 1318, 1520, 2107, 2251, 2422, 2443
Bme1390I CCNGG 5 cut(s) 109, 146, 260, 1199, 2166
Bme18I GGWCC 1 cut(s) 758
BmeT110I CYCGRG 3 cut(s) 137, 627, 2133
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 620, 758
BmiI GGNNCC 7 cut(s) 621, 760, 1794, 1830, 2026, 2226, 2397
BmrFI CCNGG 5 cut(s) 109, 146, 260, 1199, 2166
BmsI GCATC 4 cut(s) 1148, 1957, 2062, 2342
BoxI GACNNNNGTC 1 cut(s) 240
BpiI GAAGAC 4 cut(s) 397, 701, 1457, 2303
BpmI CTGGAG 4 cut(s) 143, 2142, 2310, 2481
Bpu10I CCTNAGC 5 cut(s) 1305, 2109, 2319, 2373, 2475
Bpu14I TTCGAA 1 cut(s) 2042
BpuMI CCSGG 2 cut(s) 260, 2166
BsaAI YACGTR 2 cut(s) 584, 2536
BsaBI GATNNNNATC 4 cut(s) 939, 1697, 1827, 2223
BsaHI GRCGYC 3 cut(s) 39, 72, 369
BsaI GGTCTC 2 cut(s) 1104, 1653
BsaJI CCNNGG 6 cut(s) 107, 144, 517, 1198, 1253, 2134
BsaWI WCCGGW 1 cut(s) 421
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 1635, 1665
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 481, 1354
Bse118I RCCGGY 2 cut(s) 227, 1215
Bse1I ACTGG 5 cut(s) 160, 703, 970, 1550, 2293
Bse21I CCTNAGG 1 cut(s) 1722
Bse3DI GCAATG 1 cut(s) 2496
Bse8I GATNNNNATC 4 cut(s) 939, 1697, 1827, 2223
BseBI CCWGG 3 cut(s) 109, 146, 1199
BseDI CCNNGG 6 cut(s) 107, 144, 517, 1198, 1253, 2134
BseGI GGATG 5 cut(s) 382, 1163, 1395, 1828, 2500
BseJI GATNNNNATC 4 cut(s) 939, 1697, 1827, 2223
BseLI CCNNNNNNNGG 3 cut(s) 29, 481, 1354
BseMI GCAATG 1 cut(s) 2496
BseMII CTCAG 9 cut(s) 1075, 1296, 1736, 1781, 1791, 2123, 2333, 2387, 2489
BseNI ACTGG 5 cut(s) 160, 703, 970, 1550, 2293
BseRI GAGGAG 5 cut(s) 319, 322, 359, 362, 1817
BseSI GKGCMC 5 cut(s) 173, 691, 853, 901, 2029
BseXI GCAGC 7 cut(s) 103, 1328, 1505, 2092, 2236, 2407, 2428
BseYI CCCAGC 3 cut(s) 816, 878, 1406
BsgI GTGCAG 3 cut(s) 163, 1530, 2516
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 5 cut(s) 131, 149, 430, 622, 661
BshNI GGYRCC 1 cut(s) 2024
BsiHKAI GWGCWC 4 cut(s) 384, 691, 853, 901
BsiHKCI CYCGRG 3 cut(s) 137, 627, 2133
BsiSI CCGG 8 cut(s) 192, 203, 228, 259, 422, 662, 1216, 2165
BsiWI CGTACG 1 cut(s) 584
BslFI GGGAC 4 cut(s) 127, 197, 1370, 1932
BslI CCNNNNNNNGG 3 cut(s) 29, 481, 1354
BsmAI GTCTC 3 cut(s) 27, 1104, 1653
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 4 cut(s) 127, 197, 1370, 1932
BsmI GAATGC 2 cut(s) 671, 1427
BsnI GGCC 5 cut(s) 131, 149, 430, 622, 661
Bso31I GGTCTC 2 cut(s) 1104, 1653
BsoBI CYCGRG 3 cut(s) 137, 627, 2133
Bsp119I TTCGAA 1 cut(s) 2042
Bsp1286I GDGCHC 7 cut(s) 173, 384, 691, 853, 901, 1660, 2029
Bsp1407I TGTACA 1 cut(s) 643
Bsp19I CCATGG 1 cut(s) 1253
BspACI CCGC 8 cut(s) 150, 216, 240, 251, 327, 990, 1087, 2115
BspANI GGCC 5 cut(s) 131, 149, 430, 622, 661
BspCNI CTCAG 9 cut(s) 1074, 1297, 1735, 1780, 1792, 2122, 2332, 2386, 2488
BspFNI CGCG 1 cut(s) 278
BspHI TCATGA 1 cut(s) 1993
BspLI GGNNCC 7 cut(s) 621, 760, 1794, 1830, 2026, 2226, 2397
BspMI ACCTGC 4 cut(s) 95, 104, 2110, 2368
BspT104I TTCGAA 1 cut(s) 2042
BspT107I GGYRCC 1 cut(s) 2024
BspTI CTTAAG 1 cut(s) 1184
BspTNI GGTCTC 2 cut(s) 1104, 1653
BsrBI CCGCTC 2 cut(s) 327, 990
BsrDI GCAATG 1 cut(s) 2496
BsrFI RCCGGY 2 cut(s) 227, 1215
BsrGI TGTACA 1 cut(s) 643
BsrI ACTGG 5 cut(s) 160, 703, 970, 1550, 2293
BssAI RCCGGY 2 cut(s) 227, 1215
BssECI CCNNGG 6 cut(s) 107, 144, 517, 1198, 1253, 2134
BssNI GRCGYC 3 cut(s) 39, 72, 369
BssT1I CCWWGG 1 cut(s) 1253
Bst2UI CCWGG 3 cut(s) 109, 146, 1199
Bst4CI ACNGT 6 cut(s) 340, 903, 1333, 1489, 1647, 2364
Bst6I CTCTTC 3 cut(s) 568, 1776, 1812
BstACI GRCGYC 3 cut(s) 39, 72, 369
BstAFI CTTAAG 1 cut(s) 1184
BstAPI GCANNNNNTGC 3 cut(s) 51, 96, 1587
BstAUI TGTACA 1 cut(s) 643
BstBAI YACGTR 2 cut(s) 584, 2536
BstBI TTCGAA 1 cut(s) 2042
BstDSI CCRYGG 2 cut(s) 517, 1253
BstF5I GGATG 5 cut(s) 382, 1163, 1395, 1828, 2500
BstFNI CGCG 1 cut(s) 278
BstH2I RGCGCY 1 cut(s) 1253
BstHHI GCGC 3 cut(s) 7, 208, 1252
BstMAI GTCTC 3 cut(s) 27, 1104, 1653
BstNI CCWGG 3 cut(s) 109, 146, 1199
BstNSI RCATGY 1 cut(s) 49
BstPAI GACNNNNGTC 1 cut(s) 240
BstSCI CCNGG 5 cut(s) 107, 144, 258, 1197, 2164
BstSFI CTRYAG 3 cut(s) 678, 1162, 2204
BstSLI GKGCMC 5 cut(s) 173, 691, 853, 901, 2029
BstSNI TACGTA 1 cut(s) 584
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 7 cut(s) 103, 1328, 1505, 2092, 2236, 2407, 2428
BstV2I GAAGAC 4 cut(s) 397, 701, 1457, 2303
BstX2I RGATCY 5 cut(s) 1792, 1828, 2050, 2224, 2395
BstXI CCANNNNNNTGG 2 cut(s) 869, 1254
BstYI RGATCY 5 cut(s) 1792, 1828, 2050, 2224, 2395
Bsu36I CCTNAGG 1 cut(s) 1722
BsuRI GGCC 5 cut(s) 131, 149, 430, 622, 661
BtgI CCRYGG 2 cut(s) 517, 1253
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 5 cut(s) 382, 1163, 1395, 1828, 2500
BtsIMutI CAGTG 2 cut(s) 167, 1338
BveI ACCTGC 4 cut(s) 95, 104, 2110, 2368
CaiI CAGNNNCTG 1 cut(s) 319
CciI TCATGA 1 cut(s) 1993
CfoI GCGC 3 cut(s) 7, 208, 1252
Cfr10I RCCGGY 2 cut(s) 227, 1215
Cfr13I GGNCC 2 cut(s) 620, 758
CseI GACGC 5 cut(s) 28, 80, 206, 284, 391
Csp6I GTAC 4 cut(s) 585, 644, 1490, 2286
CviQI GTAC 4 cut(s) 585, 644, 1490, 2286
DraIII CACNNNGTG 3 cut(s) 446, 1565, 2168
EaeI YGGCCR 2 cut(s) 129, 147
Eam1104I CTCTTC 3 cut(s) 568, 1776, 1812
EarI CTCTTC 3 cut(s) 568, 1776, 1812
Eco105I TACGTA 1 cut(s) 584
Eco130I CCWWGG 1 cut(s) 1253
Eco147I AGGCCT 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 1660
Eco31I GGTCTC 2 cut(s) 1104, 1653
Eco32I GATATC 1 cut(s) 1699
Eco47I GGWCC 1 cut(s) 758
Eco57I CTGAAG 5 cut(s) 485, 1331, 1458, 1733, 1979
Eco72I CACGTG 1 cut(s) 2536
Eco81I CCTNAGG 1 cut(s) 1722
Eco88I CYCGRG 3 cut(s) 137, 627, 2133
EcoO109I RGGNCCY 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 1511
EcoRII CCWGG 3 cut(s) 107, 144, 1197
EcoRV GATATC 1 cut(s) 1699
EcoT14I CCWWGG 1 cut(s) 1253
EcoT38I GRGCYC 1 cut(s) 1660
ErhI CCWWGG 1 cut(s) 1253
Esp3I CGTCTC 1 cut(s) 27
FalI AAGNNNNNCTT 6 cut(s) 356, 388, 1336, 1368, 1574, 1606
FaqI GGGAC 4 cut(s) 127, 197, 1370, 1932
FauI CCCGC 3 cut(s) 223, 320, 1080
FauNDI CATATG 1 cut(s) 1123
FbaI TGATCA 1 cut(s) 889
FblI GTMKAC 1 cut(s) 838
Fnu4HI GCNGC 9 cut(s) 117, 150, 252, 1317, 1519, 2106, 2250, 2421, 2442
FokI GGATG 5 cut(s) 389, 1170, 1402, 1835, 2507
FriOI GRGCYC 1 cut(s) 1660
Fsp4HI GCNGC 9 cut(s) 117, 150, 252, 1317, 1519, 2106, 2250, 2421, 2442
FspBI CTAG 3 cut(s) 1284, 1424, 2274
GlaI GCGC 3 cut(s) 6, 207, 1251
GluI GCNGC 9 cut(s) 117, 150, 252, 1317, 1519, 2106, 2250, 2421, 2442
GsaI CCCAGC 3 cut(s) 820, 882, 1410
GsuI CTGGAG 4 cut(s) 143, 2142, 2310, 2481
HaeII RGCGCY 1 cut(s) 1253
HaeIII GGCC 5 cut(s) 131, 149, 430, 622, 661
HapII CCGG 8 cut(s) 192, 203, 228, 259, 422, 662, 1216, 2165
HgaI GACGC 5 cut(s) 28, 80, 206, 284, 391
HhaI GCGC 3 cut(s) 7, 208, 1252
Hin1I GRCGYC 3 cut(s) 39, 72, 369
Hin6I GCGC 3 cut(s) 5, 206, 1250
HinP1I GCGC 3 cut(s) 5, 206, 1250
HpaII CCGG 8 cut(s) 192, 203, 228, 259, 422, 662, 1216, 2165
HphI GGTGA 8 cut(s) 70, 76, 821, 1549, 1738, 2180, 2181, 2508
Hpy166II GTNNAC 8 cut(s) 223, 235, 449, 689, 839, 851, 899, 1643
Hpy8I GTNNAC 8 cut(s) 223, 235, 449, 689, 839, 851, 899, 1643
Hpy99I CGWCG 4 cut(s) 32, 200, 278, 340
HpyCH4III ACNGT 6 cut(s) 340, 903, 1333, 1489, 1647, 2364
HpyCH4IV ACGT 6 cut(s) 210, 369, 583, 685, 1954, 2535
HpySE526I ACGT 6 cut(s) 210, 369, 583, 685, 1954, 2535
Hsp92I GRCGYC 3 cut(s) 39, 72, 369
HspAI GCGC 3 cut(s) 5, 206, 1250
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
Ksp22I TGATCA 1 cut(s) 889
LmnI GCTCC 8 cut(s) 205, 289, 316, 1012, 2080, 2123, 2291, 2462
Lsp1109I GCAGC 7 cut(s) 103, 1328, 1505, 2092, 2236, 2407, 2428
LweI GCATC 4 cut(s) 1148, 1957, 2062, 2342
MaeI CTAG 3 cut(s) 1284, 1424, 2274
MaeII ACGT 6 cut(s) 210, 369, 583, 685, 1954, 2535
MaeIII GTNAC 9 cut(s) 58, 100, 417, 433, 545, 733, 1552, 1621, 1755
MbiI CCGCTC 2 cut(s) 327, 990
MflI RGATCY 5 cut(s) 1792, 1828, 2050, 2224, 2395
MhlI GDGCHC 7 cut(s) 173, 384, 691, 853, 901, 1660, 2029
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 6 cut(s) 454, 525, 1511, 1524, 1975, 2016
MluNI TGGCCA 1 cut(s) 131
MlyI GAGTC 8 cut(s) 55, 149, 1053, 1203, 1763, 1814, 1954, 1984
MmeI TCCRAC 5 cut(s) 89, 466, 1081, 1171, 1820
Mox20I TGGCCA 1 cut(s) 131
MreI CGCCGGCG 1 cut(s) 227
MroNI GCCGGC 1 cut(s) 227
MroXI GAANNNNTTC 4 cut(s) 470, 1511, 2185, 2512
MscI TGGCCA 1 cut(s) 131
MseI TTAA 2 cut(s) 1185, 1844
MslI CAYNNNNRTG 1 cut(s) 1998
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 3 cut(s) 1518, 2249, 2420
MspCI CTTAAG 1 cut(s) 1184
MspI CCGG 8 cut(s) 192, 203, 228, 259, 422, 662, 1216, 2165
MspR9I CCNGG 5 cut(s) 109, 146, 260, 1199, 2166
Mva1269I GAATGC 2 cut(s) 671, 1427
MvaI CCWGG 3 cut(s) 109, 146, 1199
MvnI CGCG 1 cut(s) 278
NaeI GCCGGC 1 cut(s) 229
NciI CCSGG 2 cut(s) 260, 2166
NcoI CCATGG 1 cut(s) 1253
NdeI CATATG 1 cut(s) 1123
NgoMIV GCCGGC 1 cut(s) 227
NlaIV GGNNCC 7 cut(s) 621, 760, 1794, 1830, 2026, 2226, 2397
NmeAIII GCCGAG 1 cut(s) 100
NmuCI GTSAC 5 cut(s) 58, 100, 733, 1621, 1755
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 2042
PaeI GCATGC 1 cut(s) 49
PagI TCATGA 1 cut(s) 1993
PaqCI CACCTGC 2 cut(s) 95, 2368
PceI AGGCCT 1 cut(s) 430
PcsI WCGNNNNNNNCGW 4 cut(s) 36, 216, 1651, 2541
PctI GAATGC 2 cut(s) 671, 1427
PdiI GCCGGC 1 cut(s) 229
PdmI GAANNNNTTC 4 cut(s) 470, 1511, 2185, 2512
PfeI GAWTC 4 cut(s) 555, 739, 1717, 1856
Pfl23II CGTACG 1 cut(s) 584
PflFI GACNNNGTC 1 cut(s) 38
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 9 cut(s) 118, 151, 253, 1318, 1520, 2107, 2251, 2422, 2443
PleI GAGTC 8 cut(s) 55, 148, 1053, 1202, 1762, 1813, 1953, 1984
PmaCI CACGTG 1 cut(s) 2536
PmlI CACGTG 1 cut(s) 2536
PpsI GAGTC 8 cut(s) 55, 148, 1053, 1202, 1762, 1813, 1953, 1984
Ppu21I YACGTR 2 cut(s) 584, 2536
PshAI GACNNNNGTC 1 cut(s) 240
Psp6I CCWGG 3 cut(s) 107, 144, 1197
PspCI CACGTG 1 cut(s) 2536
PspFI CCCAGC 3 cut(s) 816, 878, 1406
PspGI CCWGG 3 cut(s) 107, 144, 1197
PspLI CGTACG 1 cut(s) 584
PspN4I GGNNCC 7 cut(s) 621, 760, 1794, 1830, 2026, 2226, 2397
PspPI GGNCC 2 cut(s) 620, 758
PstNI CAGNNNCTG 1 cut(s) 319
PsuI RGATCY 5 cut(s) 1792, 1828, 2050, 2224, 2395
PsyI GACNNNGTC 1 cut(s) 38
PvuII CAGCTG 3 cut(s) 1518, 2249, 2420
RsaI GTAC 4 cut(s) 586, 645, 1491, 2287
RsaNI GTAC 4 cut(s) 585, 644, 1490, 2286
RseI CAYNNNNRTG 1 cut(s) 1998
SaqAI TTAA 2 cut(s) 1185, 1844
SatI GCNGC 9 cut(s) 117, 150, 252, 1317, 1519, 2106, 2250, 2421, 2442
Sau96I GGNCC 2 cut(s) 620, 758
SchI GAGTC 8 cut(s) 55, 149, 1053, 1203, 1763, 1814, 1954, 1984
ScrFI CCNGG 5 cut(s) 109, 146, 260, 1199, 2166
SduI GDGCHC 7 cut(s) 173, 384, 691, 853, 901, 1660, 2029
SfaNI GCATC 4 cut(s) 1148, 1957, 2062, 2342
SfcI CTRYAG 3 cut(s) 678, 1162, 2204
SfuI TTCGAA 1 cut(s) 2042
SgrAI CRCCGGYG 1 cut(s) 227
SinI GGWCC 1 cut(s) 758
SmiMI CAYNNNNRTG 1 cut(s) 1998
SmlI CTYRAG 1 cut(s) 1184
SmoI CTYRAG 1 cut(s) 1184
SnaBI TACGTA 1 cut(s) 584
SphI GCATGC 1 cut(s) 49
Sse9I AATT 6 cut(s) 454, 525, 1511, 1524, 1975, 2016
SseBI AGGCCT 1 cut(s) 430
SsiI CCGC 8 cut(s) 150, 216, 240, 251, 327, 990, 1087, 2115
SspMI CTAG 3 cut(s) 1284, 1424, 2274
StuI AGGCCT 1 cut(s) 430
StyD4I CCNGG 5 cut(s) 107, 144, 258, 1197, 2164
StyI CCWWGG 1 cut(s) 1253
TaaI ACNGT 6 cut(s) 340, 903, 1333, 1489, 1647, 2364
TaiI ACGT 6 cut(s) 213, 372, 586, 688, 1957, 2538
TaqI TCGA 4 cut(s) 267, 1279, 1752, 2042
TasI AATT 6 cut(s) 454, 525, 1511, 1524, 1975, 2016
TatI WGTACW 1 cut(s) 643
TauI GCSGC 2 cut(s) 152, 254
TfiI GAWTC 4 cut(s) 555, 739, 1717, 1856
Tru1I TTAA 2 cut(s) 1185, 1844
Tru9I TTAA 2 cut(s) 1185, 1844
TscAI CASTG 2 cut(s) 167, 1338
TseFI GTSAC 5 cut(s) 58, 100, 733, 1621, 1755
TseI GCWGC 7 cut(s) 116, 1316, 1518, 2105, 2249, 2420, 2441
Tsp45I GTSAC 5 cut(s) 58, 100, 733, 1621, 1755
TspGWI ACGGA 2 cut(s) 45, 302
TspRI CASTG 2 cut(s) 167, 1338
Tth111I GACNNNGTC 1 cut(s) 38
Vha464I CTTAAG 1 cut(s) 1184
VneI GTGCAC 3 cut(s) 687, 849, 897
VpaK11BI GGWCC 1 cut(s) 758
XapI RAATTY 4 cut(s) 525, 1511, 1524, 2016
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 838
XmnI GAANNNNTTC 4 cut(s) 470, 1511, 2185, 2512
XspI CTAG 3 cut(s) 1284, 1424, 2274
ZraI GACGTC 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.