RchiOBHm_Chr2g0144901

metallothionein-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000858
Physical Location & Seq
Forward (+)
62456914 .. 62457826
913 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 507 bp
ATGAAGTTGGGATCTATGAGCAGAGAGCCCCATTGCTTGCACACGCACTTGAATCGAACCAAAGACTTCGCCGGCAGCCGTTTCAGGATGTCGATGATGATATCTGGAATCTCCAGTTTCAGGTTCTGGGTTGTTATTGTTTCATCTTCTTCTCTTGCATCAAACATGTTTGGTTTATCACTCGATTCGGGTTTTGGGTTTGGTTCAGGGTCGATACATCTCCATCTTCTTCTTCTTGCAGAGGCGTCGGCTTCTGGGTTTTTTTTTTTCTTTCTCGGCGGTGGTTGCGGAGGCTGTGGTAACTATCCGGACATCACAGAGCAGAGCTCTGCCTCTGAGACTCTTGTCATGGGTGTCGTGGGAACACAGAAACTGAACTATGGACGGCCTGAGGCTGGTGTGGCTATCGACGGAGGCTCCGGTGGCTGCAAGTGTGTCAACTGCACCTACAACCTTGCAATTGCAAGTGAGCGAGGTCTTGAAGCTTTAATGGAGTCTGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

168

Amino Acids

17.63

Weight (kDa)

6.49

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_2 PF01439 93 - 149 1.4e-06 Metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 2 cut(s) 279, 288
AclWI GGATC 1 cut(s) 19
AcyI GRCGYC 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 120, 395
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 2 cut(s) 52, 482
AluBI AGCT 2 cut(s) 327, 485
AluI AGCT 2 cut(s) 327, 485
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 1 cut(s) 19
AlwNI CAGNNNCTG 2 cut(s) 126, 373
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 2 cut(s) 75, 426
AxyI CCTNAGG 1 cut(s) 390
BanII GRGCYC 2 cut(s) 30, 329
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 2 cut(s) 87, 413
BccI CCATC 1 cut(s) 231
BceAI ACGGC 2 cut(s) 63, 401
BcgI CGANNNNNNTGC 4 cut(s) 35, 69, 228, 262
BcoDI GTCTC 1 cut(s) 332
BisI GCNGC 2 cut(s) 76, 427
BlsI GCNGC 2 cut(s) 77, 428
BmiI GGNNCC 1 cut(s) 418
BmsI GCATC 1 cut(s) 167
BoxI GACNNNNGTC 1 cut(s) 344
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 245
BsaWI WCCGGW 2 cut(s) 307, 419
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 395
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 114
Bse21I CCTNAGG 1 cut(s) 390
Bse3DI GCAATG 1 cut(s) 31
BseAI TCCGGA 1 cut(s) 307
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 2 cut(s) 120, 395
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 2 cut(s) 327, 381
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 87, 413
BsgI GTGCAG 1 cut(s) 427
BshFI GGCC 1 cut(s) 388
BsiHKAI GWGCWC 1 cut(s) 329
BsiSI CCGG 3 cut(s) 72, 308, 420
BslI CCNNNNNNNGG 2 cut(s) 120, 395
BsmAI GTCTC 1 cut(s) 332
BsnI GGCC 1 cut(s) 388
Bsp1286I GDGCHC 2 cut(s) 30, 329
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 1 cut(s) 11
BspACI CCGC 2 cut(s) 279, 288
BspANI GGCC 1 cut(s) 388
BspCNI CTCAG 2 cut(s) 328, 382
BspEI TCCGGA 1 cut(s) 307
BspLI GGNNCC 1 cut(s) 418
BspPI GGATC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 71
BssMI GATC 1 cut(s) 11
BssNI GRCGYC 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 38, 73
BstDEI CTNAG 2 cut(s) 336, 390
BstF5I GGATG 1 cut(s) 93
BstKTI GATC 1 cut(s) 14
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 1 cut(s) 11
BstMWI GCNNNNNNNGC 3 cut(s) 285, 401, 423
BstNSI RCATGY 1 cut(s) 169
BstPAI GACNNNNGTC 1 cut(s) 344
BstV1I GCAGC 2 cut(s) 87, 413
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
Bsu36I CCTNAGG 1 cut(s) 390
BsuRI GGCC 1 cut(s) 388
BtsCI GGATG 1 cut(s) 93
Cac8I GCNNGC 2 cut(s) 38, 73
CaiI CAGNNNCTG 2 cut(s) 126, 373
Cfr10I RCCGGY 1 cut(s) 71
CseI GACGC 1 cut(s) 234
CviAII CATG 2 cut(s) 166, 349
DdeI CTNAG 2 cut(s) 336, 390
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
Ecl136II GAGCTC 1 cut(s) 327
Eco24I GRGCYC 2 cut(s) 30, 329
Eco32I GATATC 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 327
Eco81I CCTNAGG 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 327
EcoRV GATATC 1 cut(s) 102
EcoT38I GRGCYC 2 cut(s) 30, 329
FaeI CATG 2 cut(s) 169, 352
FaiI YATR 4 cut(s) 17, 167, 350, 381
FatI CATG 2 cut(s) 165, 348
Fnu4HI GCNGC 2 cut(s) 76, 427
FokI GGATG 1 cut(s) 100
FriOI GRGCYC 2 cut(s) 30, 329
Fsp4HI GCNGC 2 cut(s) 76, 427
GluI GCNGC 2 cut(s) 76, 427
GsuI CTGGAG 1 cut(s) 97
HaeIII GGCC 1 cut(s) 388
HapII CCGG 3 cut(s) 72, 308, 420
HgaI GACGC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 2 cut(s) 169, 352
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 483
HinfI GANTC 5 cut(s) 52, 108, 185, 340, 494
HpaII CCGG 3 cut(s) 72, 308, 420
Hpy166II GTNNAC 1 cut(s) 439
Hpy188I TCNGA 1 cut(s) 337
Hpy188III TCNNGA 4 cut(s) 85, 105, 308, 479
Hpy8I GTNNAC 1 cut(s) 439
Hpy99I CGWCG 2 cut(s) 250, 413
HpyCH4V TGCA 7 cut(s) 40, 158, 239, 429, 444, 458, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 285, 401, 423
HpyF3I CTNAG 2 cut(s) 336, 390
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 2 cut(s) 169, 352
Kpn2I TCCGGA 1 cut(s) 307
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 11
LmnI GCTCC 1 cut(s) 422
Lsp1109I GCAGC 2 cut(s) 87, 413
LweI GCATC 1 cut(s) 167
MaeIII GTNAC 1 cut(s) 299
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MboII GAAGA 5 cut(s) 138, 141, 218, 221, 224
MfeI CAATTG 1 cut(s) 459
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 2 cut(s) 30, 329
MluCI AATT 1 cut(s) 459
MlyI GAGTC 2 cut(s) 334, 503
MnlI CCTC 6 cut(s) 235, 284, 343, 385, 407, 467
MroI TCCGGA 1 cut(s) 307
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 1 cut(s) 488
MspI CCGG 3 cut(s) 72, 308, 420
MunI CAATTG 1 cut(s) 459
MwoI GCNNNNNNNGC 3 cut(s) 285, 401, 423
NaeI GCCGGC 1 cut(s) 73
NdeII GATC 1 cut(s) 11
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 2 cut(s) 169, 352
NlaIV GGNNCC 1 cut(s) 418
NmeAIII GCCGAG 1 cut(s) 255
NspI RCATGY 1 cut(s) 169
PciI ACATGT 1 cut(s) 165
PdiI GCCGGC 1 cut(s) 73
PfeI GAWTC 3 cut(s) 52, 108, 185
PkrI GCNGC 2 cut(s) 77, 428
PleI GAGTC 2 cut(s) 334, 502
PpsI GAGTC 2 cut(s) 334, 502
PscI ACATGT 1 cut(s) 165
PshAI GACNNNNGTC 1 cut(s) 344
Psp124BI GAGCTC 1 cut(s) 329
PspN4I GGNNCC 1 cut(s) 418
PstNI CAGNNNCTG 2 cut(s) 126, 373
PsuI RGATCY 1 cut(s) 11
SacI GAGCTC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 488
SatI GCNGC 2 cut(s) 76, 427
Sau3AI GATC 1 cut(s) 11
SchI GAGTC 2 cut(s) 334, 503
SduI GDGCHC 2 cut(s) 30, 329
SetI ASST 6 cut(s) 125, 329, 449, 456, 478, 487
SfaNI GCATC 1 cut(s) 167
Sse9I AATT 1 cut(s) 459
SsiI CCGC 2 cut(s) 279, 288
SstI GAGCTC 1 cut(s) 329
TaqI TCGA 5 cut(s) 55, 92, 183, 212, 408
TasI AATT 1 cut(s) 459
TfiI GAWTC 3 cut(s) 52, 108, 185
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TseI GCWGC 2 cut(s) 75, 426
TspDTI ATGAA 2 cut(s) 17, 132
TspGWI ACGGA 1 cut(s) 426
XceI RCATGY 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.