RchiOBHm_Chr4g0417601

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001337
Physical Location & Seq
Reverse (-)
42897013 .. 42897753
741 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38768

Sequence Viewer

Length: 507 bp
ATGATTCATGATGACGATATCCACTATTCAGATGTTAGAAGATTGCTGGATTGCAGAATGGATTACTGTGAAGAAGAAAGGGAAGACATGGAGTTGCCGCTATTTGACTTCACCACTATTGCTGATGCCACTAATAACTTTTCAAGCAACAACAAACTGGGACAAGGCAGTTTTGGACCTGTGTACAAGGGTACATTGATAGGAGGGAAAGAAATAGCTGTAAAGAGGCGATCCAAGGAATCTGGTCAAGGAATGAGGGAGTTCAAAAATGAAGTTATACTGATAGCCAAACTTCAGCACCGTAATCTTGTGAAGCTTCTGGGCTGTTGCATTCAAAATGATGAAAAAATGTTAATATATGAATACACGTCCAACAGGAGCTTGGACTTCTTTATATTTGGTATGATCCTTTCCAAGCTCAACTACATGGATTTACAGTCATATCATCCTCCATTGGTTTTACTTAATCCACTAGGATTTATTCATAGTGCCTTATATTTGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

168

Amino Acids

19.4

Weight (kDa)

5.72

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 48 - 132 2.2e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 50 - 132 1.9e-15 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 98
AclWI GGATC 2 cut(s) 225, 400
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 2 cut(s) 185, 193
AflIII ACRYGT 1 cut(s) 366
AgsI TTSAA 3 cut(s) 144, 265, 335
AjiI CACGTC 1 cut(s) 369
AluBI AGCT 4 cut(s) 218, 316, 381, 418
AluI AGCT 4 cut(s) 218, 316, 381, 418
AlwI GGATC 2 cut(s) 225, 400
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 90
BfaI CTAG 1 cut(s) 473
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme18I GGWCC 1 cut(s) 176
BmgBI CACGTC 1 cut(s) 369
BmgT120I GGNCC 1 cut(s) 176
BmrI ACTGGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 115
BmuI ACTGGG 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 234
Bse1I ACTGG 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 1 cut(s) 445
BseNI ACTGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 330
Bsp1407I TGTACA 1 cut(s) 183
Bsp143I GATC 2 cut(s) 230, 405
BspACI CCGC 1 cut(s) 98
BspHI TCATGA 1 cut(s) 7
BspPI GGATC 2 cut(s) 225, 400
BsrGI TGTACA 1 cut(s) 183
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 2 cut(s) 230, 405
BssT1I CCWWGG 1 cut(s) 234
Bst4CI ACNGT 3 cut(s) 68, 302, 438
BstAUI TGTACA 1 cut(s) 183
BstF5I GGATG 1 cut(s) 445
BstKTI GATC 2 cut(s) 233, 408
BstMBI GATC 2 cut(s) 230, 405
BstV2I GAAGAC 1 cut(s) 90
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 1 cut(s) 445
CciI TCATGA 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 184, 192
CviAII CATG 3 cut(s) 8, 88, 427
CviJI RGCY 6 cut(s) 218, 287, 316, 324, 381, 418
CviKI_1 RGCY 6 cut(s) 218, 287, 316, 324, 381, 418
CviQI GTAC 2 cut(s) 184, 192
DpnI GATC 2 cut(s) 232, 407
DpnII GATC 2 cut(s) 230, 405
Eco130I CCWWGG 1 cut(s) 234
Eco32I GATATC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 278
EcoRV GATATC 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 3 cut(s) 11, 91, 430
FaqI GGGAC 1 cut(s) 174
FatI CATG 3 cut(s) 7, 87, 426
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 432
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 1 cut(s) 473
GluI GCNGC 1 cut(s) 98
Hin1II CATG 3 cut(s) 11, 91, 430
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 2 cut(s) 4, 239
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 1 cut(s) 31
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 1 cut(s) 184
HpyCH4III ACNGT 3 cut(s) 68, 302, 438
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 2 cut(s) 54, 330
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 3 cut(s) 11, 91, 430
Kzo9I GATC 2 cut(s) 230, 405
LmnI GCTCC 1 cut(s) 378
LpnPI CCDG 6 cut(s) 32, 143, 192, 228, 305, 361
LweI GCATC 1 cut(s) 115
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 368
MalI GATC 2 cut(s) 232, 407
MboI GATC 2 cut(s) 230, 405
MboII GAAGA 4 cut(s) 51, 83, 86, 95
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 4 cut(s) 197, 219, 249, 459
MseI TTAA 2 cut(s) 353, 465
Mva1269I GAATGC 1 cut(s) 330
NdeII GATC 2 cut(s) 230, 405
NlaIII CATG 3 cut(s) 11, 91, 430
PagI TCATGA 1 cut(s) 7
PctI GAATGC 1 cut(s) 330
PfeI GAWTC 2 cut(s) 4, 239
PkrI GCNGC 1 cut(s) 99
PspPI GGNCC 1 cut(s) 176
RsaI GTAC 2 cut(s) 185, 193
RsaNI GTAC 2 cut(s) 184, 192
SaqAI TTAA 2 cut(s) 353, 465
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 2 cut(s) 230, 405
Sau96I GGNCC 1 cut(s) 176
SetI ASST 6 cut(s) 181, 220, 318, 371, 383, 420
SfaNI GCATC 1 cut(s) 115
SinI GGWCC 1 cut(s) 176
SsiI CCGC 1 cut(s) 98
SspMI CTAG 1 cut(s) 473
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 3 cut(s) 68, 302, 438
TaiI ACGT 1 cut(s) 371
TatI WGTACW 1 cut(s) 183
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 2 cut(s) 4, 239
Tru1I TTAA 2 cut(s) 353, 465
Tru9I TTAA 2 cut(s) 353, 465
TspDTI ATGAA 4 cut(s) 285, 357, 375, 473
VpaK11BI GGWCC 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 379
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.