divergent subfamily of APPLE domains
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 98, 168 |
| AclWI | GGATC | 2 cut(s) | 225, 400 |
| AcuI | CTGAAG | 1 cut(s) | 278 |
| AfaI | GTAC | 2 cut(s) | 185, 193 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 382 |
| AflIII | ACRYGT | 1 cut(s) | 366 |
| AgsI | TTSAA | 3 cut(s) | 144, 265, 335 |
| AjiI | CACGTC | 1 cut(s) | 369 |
| AluBI | AGCT | 4 cut(s) | 218, 316, 381, 418 |
| AluI | AGCT | 4 cut(s) | 218, 316, 381, 418 |
| AlwI | GGATC | 2 cut(s) | 225, 400 |
| ApeKI | GCWGC | 3 cut(s) | 313, 324, 327 |
| AspS9I | GGNCC | 1 cut(s) | 176 |
| AsuHPI | GGTGA | 1 cut(s) | 103 |
| AvaII | GGWCC | 1 cut(s) | 176 |
| BbsI | GAAGAC | 1 cut(s) | 90 |
| BbvI | GCAGC | 3 cut(s) | 311, 314, 325 |
| BfaI | CTAG | 1 cut(s) | 473 |
| BisI | GCNGC | 4 cut(s) | 98, 314, 325, 328 |
| BlsI | GCNGC | 4 cut(s) | 99, 315, 326, 329 |
| Bme18I | GGWCC | 1 cut(s) | 176 |
| BmgBI | CACGTC | 1 cut(s) | 369 |
| BmgT120I | GGNCC | 1 cut(s) | 176 |
| BmrI | ACTGGG | 1 cut(s) | 167 |
| BmsI | GCATC | 1 cut(s) | 115 |
| BmuI | ACTGGG | 1 cut(s) | 167 |
| BpiI | GAAGAC | 1 cut(s) | 90 |
| BsaJI | CCNNGG | 1 cut(s) | 234 |
| BsaWI | WCCGGW | 1 cut(s) | 375 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 382 |
| Bse1I | ACTGG | 1 cut(s) | 162 |
| BseDI | CCNNGG | 1 cut(s) | 234 |
| BseGI | GGATG | 1 cut(s) | 445 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 382 |
| BseNI | ACTGG | 1 cut(s) | 162 |
| BseXI | GCAGC | 3 cut(s) | 311, 314, 325 |
| BsgI | GTGCAG | 1 cut(s) | 332 |
| BsiSI | CCGG | 1 cut(s) | 376 |
| BslFI | GGGAC | 1 cut(s) | 174 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 382 |
| BsmFI | GGGAC | 1 cut(s) | 174 |
| BsmI | GAATGC | 1 cut(s) | 330 |
| Bsp1407I | TGTACA | 1 cut(s) | 183 |
| Bsp143I | GATC | 2 cut(s) | 230, 405 |
| BspACI | CCGC | 2 cut(s) | 98, 168 |
| BspHI | TCATGA | 1 cut(s) | 7 |
| BspPI | GGATC | 2 cut(s) | 225, 400 |
| BsrGI | TGTACA | 1 cut(s) | 183 |
| BsrI | ACTGG | 1 cut(s) | 162 |
| BssECI | CCNNGG | 1 cut(s) | 234 |
| BssMI | GATC | 2 cut(s) | 230, 405 |
| BssT1I | CCWWGG | 1 cut(s) | 234 |
| Bst4CI | ACNGT | 3 cut(s) | 68, 302, 438 |
| BstAUI | TGTACA | 1 cut(s) | 183 |
| BstF5I | GGATG | 1 cut(s) | 445 |
| BstKTI | GATC | 2 cut(s) | 233, 408 |
| BstMBI | GATC | 2 cut(s) | 230, 405 |
| BstV1I | GCAGC | 3 cut(s) | 311, 314, 325 |
| BstV2I | GAAGAC | 1 cut(s) | 90 |
| BtrI | CACGTC | 1 cut(s) | 369 |
| BtsCI | GGATG | 1 cut(s) | 445 |
| BtsIMutI | CAGTG | 1 cut(s) | 129 |
| CciI | TCATGA | 1 cut(s) | 7 |
| Cfr13I | GGNCC | 1 cut(s) | 176 |
| Csp6I | GTAC | 2 cut(s) | 184, 192 |
| CviAII | CATG | 3 cut(s) | 8, 88, 427 |
| CviJI | RGCY | 6 cut(s) | 218, 287, 316, 324, 381, 418 |
| CviKI_1 | RGCY | 6 cut(s) | 218, 287, 316, 324, 381, 418 |
| CviQI | GTAC | 2 cut(s) | 184, 192 |
| DpnI | GATC | 2 cut(s) | 232, 407 |
| DpnII | GATC | 2 cut(s) | 230, 405 |
| Eco130I | CCWWGG | 1 cut(s) | 234 |
| Eco32I | GATATC | 1 cut(s) | 19 |
| Eco47I | GGWCC | 1 cut(s) | 176 |
| Eco57I | CTGAAG | 1 cut(s) | 278 |
| EcoRV | GATATC | 1 cut(s) | 19 |
| EcoT14I | CCWWGG | 1 cut(s) | 234 |
| ErhI | CCWWGG | 1 cut(s) | 234 |
| FaeI | CATG | 3 cut(s) | 11, 91, 430 |
| FaqI | GGGAC | 1 cut(s) | 174 |
| FatI | CATG | 3 cut(s) | 7, 87, 426 |
| Fnu4HI | GCNGC | 4 cut(s) | 98, 314, 325, 328 |
| FokI | GGATG | 1 cut(s) | 432 |
| Fsp4HI | GCNGC | 4 cut(s) | 98, 314, 325, 328 |
| FspBI | CTAG | 1 cut(s) | 473 |
| GluI | GCNGC | 4 cut(s) | 98, 314, 325, 328 |
| HapII | CCGG | 1 cut(s) | 376 |
| Hin1II | CATG | 3 cut(s) | 11, 91, 430 |
| HinfI | GANTC | 2 cut(s) | 4, 239 |
| HpaII | CCGG | 1 cut(s) | 376 |
| HphI | GGTGA | 1 cut(s) | 103 |
| Hpy166II | GTNNAC | 1 cut(s) | 184 |
| Hpy188I | TCNGA | 1 cut(s) | 31 |
| Hpy188III | TCNNGA | 1 cut(s) | 8 |
| Hpy8I | GTNNAC | 1 cut(s) | 184 |
| HpyCH4III | ACNGT | 3 cut(s) | 68, 302, 438 |
| HpyCH4IV | ACGT | 1 cut(s) | 368 |
| HpyCH4V | TGCA | 3 cut(s) | 54, 313, 330 |
| HpySE526I | ACGT | 1 cut(s) | 368 |
| Hsp92II | CATG | 3 cut(s) | 11, 91, 430 |
| Kzo9I | GATC | 2 cut(s) | 230, 405 |
| LmnI | GCTCC | 1 cut(s) | 378 |
| LpnPI | CCDG | 5 cut(s) | 32, 143, 192, 228, 389 |
| Lsp1109I | GCAGC | 3 cut(s) | 311, 314, 325 |
| LweI | GCATC | 1 cut(s) | 115 |
| MaeI | CTAG | 1 cut(s) | 473 |
| MaeII | ACGT | 1 cut(s) | 368 |
| MalI | GATC | 2 cut(s) | 232, 407 |
| MboI | GATC | 2 cut(s) | 230, 405 |
| MboII | GAAGA | 4 cut(s) | 51, 83, 86, 95 |
| MmeI | TCCRAC | 1 cut(s) | 396 |
| MnlI | CCTC | 4 cut(s) | 197, 219, 249, 459 |
| MseI | TTAA | 2 cut(s) | 353, 465 |
| MspI | CCGG | 1 cut(s) | 376 |
| Mva1269I | GAATGC | 1 cut(s) | 330 |
| NdeII | GATC | 2 cut(s) | 230, 405 |
| NlaIII | CATG | 3 cut(s) | 11, 91, 430 |
| PagI | TCATGA | 1 cut(s) | 7 |
| PctI | GAATGC | 1 cut(s) | 330 |
| PfeI | GAWTC | 2 cut(s) | 4, 239 |
| PkrI | GCNGC | 4 cut(s) | 99, 315, 326, 329 |
| PspPI | GGNCC | 1 cut(s) | 176 |
| RsaI | GTAC | 2 cut(s) | 185, 193 |
| RsaNI | GTAC | 2 cut(s) | 184, 192 |
| SaqAI | TTAA | 2 cut(s) | 353, 465 |
| SatI | GCNGC | 4 cut(s) | 98, 314, 325, 328 |
| Sau3AI | GATC | 2 cut(s) | 230, 405 |
| Sau96I | GGNCC | 1 cut(s) | 176 |
| SetI | ASST | 6 cut(s) | 181, 220, 318, 371, 383, 420 |
| SfaNI | GCATC | 1 cut(s) | 115 |
| SinI | GGWCC | 1 cut(s) | 176 |
| SsiI | CCGC | 2 cut(s) | 98, 168 |
| SspMI | CTAG | 1 cut(s) | 473 |
| StyI | CCWWGG | 1 cut(s) | 234 |
| TaaI | ACNGT | 3 cut(s) | 68, 302, 438 |
| TaiI | ACGT | 1 cut(s) | 371 |
| TatI | WGTACW | 1 cut(s) | 183 |
| TauI | GCSGC | 1 cut(s) | 100 |
| TfiI | GAWTC | 2 cut(s) | 4, 239 |
| Tru1I | TTAA | 2 cut(s) | 353, 465 |
| Tru9I | TTAA | 2 cut(s) | 353, 465 |
| TscAI | CASTG | 1 cut(s) | 136 |
| TseI | GCWGC | 3 cut(s) | 313, 324, 327 |
| TspDTI | ATGAA | 4 cut(s) | 285, 357, 375, 473 |
| TspRI | CASTG | 1 cut(s) | 136 |
| VpaK11BI | GGWCC | 1 cut(s) | 176 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 379 |
| XspI | CTAG | 1 cut(s) | 473 |