RchiOBHm_Chr5g0025141

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001337
Physical Location & Seq
Forward (+)
19136450 .. 19137337
888 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30483

Sequence Viewer

Length: 645 bp
ATGAGAATGGAGACTACTTCAGTTACACTGTCGTTGGTGATCCAAATGGCAGATCAGCCTGGGTGCTGGACACAGGTGGGGAGCTTTATGAAGTTATCTATAACATTTCTATTGCGTCAGCAGGTCACTGTGATGGCTATAAAAATGGGACAATATTTAAGTGTATTTAGTTATGCATCTATTATAGCTGCCACTTGCAACTTCTCTAATGAAAATAAGCTCGGAGAAGGGGGTTTTGGACCTGTTTATAAGGGAATATATGAGTTGACGAATGAATTGATACTTATATATGAACTCCAGCATACAAACCTTGTCCAGCTATTTGGATTTTGCATTCATGGTGAAGAGAGGATGTTGATATACGAGTACATGCCAAACAAAAGCTTGGACTACTTTTTATTTGATTCAACTAGATGCCAGCAATTAGACTGGAACAACCGTTTCAGCATAATTGAAGGAATTGCTCAAGGTTTACTTTACTTGCACAAATATTCCAGAATGAGAGTAATTCATAGAGATTTGAAAGCAAGTAATGTTCTACTTGATGAAAGCATGAACCCCAAAATTTCCGATTTTGGAATGGCAAGGATTTTCACGAATGATGAACAAGAAGCAAATACTATGAAGATTGTTGGGACACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

214

Amino Acids

24.61

Weight (kDa)

6.09

Isoelectric Point (pI)

26.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 88 - 206 7.2e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 88 - 213 5.3e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 112
AclWI GGATC 1 cut(s) 34
AcsI RAATTY 1 cut(s) 564
AfaI GTAC 1 cut(s) 368
AgsI TTSAA 3 cut(s) 408, 455, 523
AjnI CCWGG 1 cut(s) 58
AjuI GAANNNNNNNTTGG 2 cut(s) 219, 251
AluBI AGCT 5 cut(s) 84, 188, 220, 319, 384
AluI AGCT 5 cut(s) 84, 188, 220, 319, 384
Alw26I GTCTC 1 cut(s) 5
AlwI GGATC 1 cut(s) 34
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 1 cut(s) 564
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 49, 353
AvaII GGWCC 1 cut(s) 239
BbvI GCAGC 1 cut(s) 175
BccI CCATC 1 cut(s) 127
BciT130I CCWGG 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 5
BfaI CTAG 1 cut(s) 411
BfuAI ACCTGC 1 cut(s) 112
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 60
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 60
BmsI GCATC 2 cut(s) 185, 404
BpmI CTGGAG 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 59
Bse1I ACTGG 1 cut(s) 434
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 1 cut(s) 357
BseNI ACTGG 1 cut(s) 434
BseXI GCAGC 1 cut(s) 175
BslFI GGGAC 1 cut(s) 162
BsmAI GTCTC 1 cut(s) 5
BsmFI GGGAC 1 cut(s) 162
BsmI GAATGC 1 cut(s) 333
Bsp143I GATC 2 cut(s) 39, 52
BspMI ACCTGC 1 cut(s) 112
BspPI GGATC 1 cut(s) 34
BsrI ACTGG 1 cut(s) 434
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 2 cut(s) 39, 52
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 4 cut(s) 30, 130, 440, 642
Bst6I CTCTTC 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 419
BstF5I GGATG 1 cut(s) 357
BstKTI GATC 2 cut(s) 42, 55
BstMAI GTCTC 1 cut(s) 5
BstMBI GATC 2 cut(s) 39, 52
BstNI CCWGG 1 cut(s) 60
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 1 cut(s) 58
BstV1I GCAGC 1 cut(s) 175
BstXI CCANNNNNNTGG 1 cut(s) 323
BtsCI GGATG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 26, 126
BveI ACCTGC 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 239
CseI GACGC 1 cut(s) 104
Csp6I GTAC 1 cut(s) 367
CviAII CATG 3 cut(s) 338, 370, 553
CviJI RGCY 7 cut(s) 58, 84, 137, 188, 220, 319, 384
CviKI_1 RGCY 7 cut(s) 58, 84, 137, 188, 220, 319, 384
CviQI GTAC 1 cut(s) 367
DpnI GATC 2 cut(s) 41, 54
DpnII GATC 2 cut(s) 39, 52
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 58
EcoT22I ATGCAT 1 cut(s) 178
FaeI CATG 3 cut(s) 341, 373, 556
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 1 cut(s) 162
FatI CATG 3 cut(s) 337, 369, 552
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 1 cut(s) 364
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 1 cut(s) 411
GluI GCNGC 1 cut(s) 189
GsuI CTGGAG 1 cut(s) 281
HgaI GACGC 1 cut(s) 104
Hin1II CATG 3 cut(s) 341, 373, 556
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 382
HinfI GANTC 1 cut(s) 404
HphI GGTGA 2 cut(s) 49, 353
Hpy166II GTNNAC 2 cut(s) 267, 473
Hpy188I TCNGA 2 cut(s) 224, 571
Hpy188III TCNNGA 2 cut(s) 495, 595
Hpy8I GTNNAC 2 cut(s) 267, 473
HpyAV CCTTC 2 cut(s) 221, 449
HpyCH4III ACNGT 4 cut(s) 30, 130, 440, 642
HpyCH4V TGCA 4 cut(s) 176, 198, 333, 484
Hsp92II CATG 3 cut(s) 341, 373, 556
Kzo9I GATC 2 cut(s) 39, 52
LmnI GCTCC 1 cut(s) 81
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 2 cut(s) 185, 404
MaeI CTAG 1 cut(s) 411
MaeIII GTNAC 2 cut(s) 22, 124
MalI GATC 2 cut(s) 41, 54
MboI GATC 2 cut(s) 39, 52
MboII GAAGA 2 cut(s) 356, 637
MluCI AATT 6 cut(s) 275, 422, 450, 459, 507, 564
MnlI CCTC 1 cut(s) 342
Mph1103I ATGCAT 1 cut(s) 178
MseI TTAA 1 cut(s) 158
MslI CAYNNNNRTG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 60
Mva1269I GAATGC 1 cut(s) 333
MvaI CCWGG 1 cut(s) 60
NdeII GATC 2 cut(s) 39, 52
NlaIII CATG 3 cut(s) 341, 373, 556
NmuCI GTSAC 1 cut(s) 124
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 1 cut(s) 373
PctI GAATGC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 404
PkrI GCNGC 1 cut(s) 190
PsiI TTATAA 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 58
PspPI GGNCC 1 cut(s) 239
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 1 cut(s) 158
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 2 cut(s) 39, 52
Sau96I GGNCC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 60
SfaNI GCATC 2 cut(s) 185, 404
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 465
SmoI CTYRAG 1 cut(s) 465
Sse9I AATT 6 cut(s) 275, 422, 450, 459, 507, 564
SspI AATATT 2 cut(s) 155, 491
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 58
TaaI ACNGT 4 cut(s) 30, 130, 440, 642
TasI AATT 6 cut(s) 275, 422, 450, 459, 507, 564
TatI WGTACW 1 cut(s) 366
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 1 cut(s) 158
Tru9I TTAA 1 cut(s) 158
TscAI CASTG 2 cut(s) 33, 133
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 124
TspRI CASTG 2 cut(s) 33, 133
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 1 cut(s) 564
XceI RCATGY 1 cut(s) 373
XspI CTAG 1 cut(s) 411
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.