RchiOBHm_Chr5g0025341

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001337
Physical Location & Seq
Forward (+)
19269878 .. 19270362
485 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30500

Sequence Viewer

Length: 315 bp
ATGGAACATGATATAAGCATTTTCAGCTACACATCTGTTCTGGCTGCCACACGCAACATCTCTGACAAAAACAAGCTCGGTGAACGGGGGTTTGGATCTGTTTATCAGGGAAAATTGGTGACAGGACAAGAAATAGCTGTGAAGAAGCTTTCCAAGCAAGGAGCCCTAGAGTTTGAGAACGAATTGATACTCGTATATGAACTCCAACATACTAACCTTGTTCGGCTTTTTGGATTTTGCATTCATGGTGAAGAGAGGATGTTAATATATGAGTACATGTCAAACAAAAAGTCTGGACTACTTTTTATTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

104

Amino Acids

11.94

Weight (kDa)

6.83

Isoelectric Point (pI)

26.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 22 - 97 3.3e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 23 - 95 5e-12 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 103
AfaI GTAC 1 cut(s) 275
AflIII ACRYGT 1 cut(s) 276
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
AluBI AGCT 4 cut(s) 27, 76, 137, 148
AluI AGCT 4 cut(s) 27, 76, 137, 148
AlwI GGATC 1 cut(s) 103
ApeKI GCWGC 1 cut(s) 44
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AsuHPI GGTGA 3 cut(s) 92, 130, 260
BanII GRGCYC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 31
BfaI CTAG 1 cut(s) 167
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 163
BseGI GGATG 1 cut(s) 264
BseXI GCAGC 1 cut(s) 31
BsmI GAATGC 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 1 cut(s) 95
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 1 cut(s) 103
BssMI GATC 1 cut(s) 95
Bst6I CTCTTC 1 cut(s) 246
BstF5I GGATG 1 cut(s) 264
BstKTI GATC 1 cut(s) 98
BstMBI GATC 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 24, 154
BstNSI RCATGY 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BtsCI GGATG 1 cut(s) 264
Csp6I GTAC 1 cut(s) 274
CviAII CATG 3 cut(s) 8, 245, 277
CviJI RGCY 7 cut(s) 27, 44, 76, 137, 148, 164, 226
CviKI_1 RGCY 7 cut(s) 27, 44, 76, 137, 148, 164, 226
CviQI GTAC 1 cut(s) 274
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco24I GRGCYC 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 166
FaeI CATG 3 cut(s) 11, 248, 280
FaiI YATR 9 cut(s) 9, 14, 196, 198, 210, 246, 268, 270, 278
FatI CATG 3 cut(s) 7, 244, 276
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 1 cut(s) 271
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 45
FspBI CTAG 1 cut(s) 167
GluI GCNGC 1 cut(s) 45
Hin1II CATG 3 cut(s) 11, 248, 280
HindIII AAGCTT 1 cut(s) 146
HphI GGTGA 3 cut(s) 92, 130, 260
Hpy166II GTNNAC 1 cut(s) 83
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 1 cut(s) 294
Hpy8I GTNNAC 1 cut(s) 83
HpyCH4V TGCA 1 cut(s) 240
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 154
Hsp92II CATG 3 cut(s) 11, 248, 280
Kzo9I GATC 1 cut(s) 95
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 4 cut(s) 26, 92, 108, 279
Lsp1109I GCAGC 1 cut(s) 31
MaeI CTAG 1 cut(s) 167
MaeIII GTNAC 1 cut(s) 118
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 2 cut(s) 154, 263
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 2 cut(s) 113, 182
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 1 cut(s) 249
MseI TTAA 1 cut(s) 263
Mva1269I GAATGC 1 cut(s) 240
MwoI GCNNNNNNNGC 2 cut(s) 24, 154
NdeII GATC 1 cut(s) 95
NlaIII CATG 3 cut(s) 11, 248, 280
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 118
NspI RCATGY 1 cut(s) 280
PciI ACATGT 1 cut(s) 276
PctI GAATGC 1 cut(s) 240
PkrI GCNGC 1 cut(s) 46
PscI ACATGT 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 163
PsuI RGATCY 1 cut(s) 95
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
SaqAI TTAA 1 cut(s) 263
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 1 cut(s) 95
SduI GDGCHC 1 cut(s) 166
SetI ASST 5 cut(s) 29, 78, 139, 150, 219
Sse9I AATT 2 cut(s) 113, 182
SspMI CTAG 1 cut(s) 167
TasI AATT 2 cut(s) 113, 182
TatI WGTACW 1 cut(s) 273
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 2 cut(s) 213, 233
XceI RCATGY 1 cut(s) 280
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.