Rroxscaffold_2G00089290

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001337
Physical Location & Seq
Forward (+)
11300427 .. 11302512
2086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00089290.1

Sequence Viewer

Length: 684 bp
ATGTCAGATCAAATGAGTCATCAACAGCTTGATAGTGAAAGACGAGTCAAAGAGCTGATAGACACGAGTGACTTCAAGGAAGAAGATGAGAAAAGCATAGATGTACCTTTTTTTGATTTGCAAAGCATACTAGACGCTACAGATAATTTCTCATTTAAAAACAAGATTGGACAAGGGGGGTATGGGCCTGTTTACAAGGGAAAATTTTCTGGAGGTCAAGAAATTGCTGTAAAGAGGCTATCAAGGGTTTCAGGACAAGGCTTACAGGAATTTAAGAATGAGGTGGTGCTAATCGCAAAACTTCAACATCGATTTCTTTATCTTCATCAAGATTCCAGATTAAGGATTATTCATAGAGATCTGAAAACTAGCAACATCCTTTTGGATGAGGAGATGAACCCTAAAATATCTGACTTCGGCTTGGCGAGGATTGTTGGAGGGAAAGAAACTGAGGCGAACACAAACACGGCATGGGGACTCTGGGCAGAGAATAAGGTTGTGGATTTAATGGACATAGATTTGCATGAAAGCTGCAACACAAATCAATTTTTCAAGTGTGTGAATATCCGGCTCTTATGCGTACAAGAAGATCCTAGTGATCGCCCCACCATGTCAAATGTTGTTGCCATGCTTGACAGTGAAACTGCAATACTCCAGTTCCTAAGCAACCGGCCTTTCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

227

Amino Acids

25.88

Weight (kDa)

5.2

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 107 - 153 1.5e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 114 - 152 2.2e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 584
AcsI RAATTY 2 cut(s) 203, 269
AfaI GTAC 2 cut(s) 105, 582
AfiI CCNNNNNNNGG 1 cut(s) 342
AgsI TTSAA 3 cut(s) 76, 305, 553
AluBI AGCT 3 cut(s) 28, 55, 531
AluI AGCT 3 cut(s) 28, 55, 531
AlwI GGATC 1 cut(s) 584
AoxI GGCC 2 cut(s) 185, 671
ApeKI GCWGC 1 cut(s) 531
ApoI RAATTY 2 cut(s) 203, 269
Asp700I GAANNNNTTC 1 cut(s) 205
AspS9I GGNCC 1 cut(s) 185
BauI CACGAG 1 cut(s) 64
BbvI GCAGC 1 cut(s) 518
BceAI ACGGC 1 cut(s) 483
BfaI CTAG 3 cut(s) 131, 369, 594
BfmI CTRYAG 1 cut(s) 138
BglII AGATCT 1 cut(s) 358
BisI GCNGC 1 cut(s) 532
BlsI GCNGC 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 185
BpmI CTGGAG 2 cut(s) 231, 638
Bpu10I CCTNAGC 1 cut(s) 662
Bsa29I ATCGAT 1 cut(s) 310
Bsc4I CCNNNNNNNGG 1 cut(s) 342
Bse118I RCCGGY 1 cut(s) 669
Bse1I ACTGG 1 cut(s) 655
BseCI ATCGAT 1 cut(s) 310
BseGI GGATG 2 cut(s) 375, 391
BseLI CCNNNNNNNGG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 518
BshFI GGCC 2 cut(s) 187, 673
BshVI ATCGAT 1 cut(s) 310
BsiSI CCGG 2 cut(s) 568, 670
BslFI GGGAC 1 cut(s) 489
BslI CCNNNNNNNGG 1 cut(s) 342
BsmFI GGGAC 1 cut(s) 489
BsnI GGCC 2 cut(s) 187, 673
Bsp143I GATC 4 cut(s) 7, 358, 589, 598
BspANI GGCC 2 cut(s) 187, 673
BspCNI CTCAG 1 cut(s) 442
BspDI ATCGAT 1 cut(s) 310
BspPI GGATC 1 cut(s) 584
BsrFI RCCGGY 1 cut(s) 669
BsrI ACTGG 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 669
BssMI GATC 4 cut(s) 7, 358, 589, 598
BssSI CACGAG 1 cut(s) 64
Bst2BI CACGAG 1 cut(s) 64
Bst4CI ACNGT 1 cut(s) 638
BstDEI CTNAG 2 cut(s) 450, 662
BstF5I GGATG 2 cut(s) 375, 391
BstKTI GATC 4 cut(s) 10, 361, 592, 601
BstMBI GATC 4 cut(s) 7, 358, 589, 598
BstSFI CTRYAG 1 cut(s) 138
BstV1I GCAGC 1 cut(s) 518
BstX2I RGATCY 2 cut(s) 358, 589
BstYI RGATCY 2 cut(s) 358, 589
Bsu15I ATCGAT 1 cut(s) 310
BsuRI GGCC 2 cut(s) 187, 673
BsuTUI ATCGAT 1 cut(s) 310
BtsCI GGATG 2 cut(s) 375, 391
BtsIMutI CAGTG 1 cut(s) 643
Cfr10I RCCGGY 1 cut(s) 669
Cfr13I GGNCC 1 cut(s) 185
ClaI ATCGAT 1 cut(s) 310
CseI GACGC 1 cut(s) 143
Csp6I GTAC 2 cut(s) 104, 581
CviAII CATG 4 cut(s) 471, 524, 610, 628
CviJI RGCY 9 cut(s) 28, 55, 187, 238, 261, 420, 531, 571, 673
CviKI_1 RGCY 9 cut(s) 28, 55, 187, 238, 261, 420, 531, 571, 673
CviQI GTAC 2 cut(s) 104, 581
DdeI CTNAG 2 cut(s) 450, 662
DpnI GATC 4 cut(s) 9, 360, 591, 600
DpnII GATC 4 cut(s) 7, 358, 589, 598
DraI TTTAAA 1 cut(s) 157
FaeI CATG 4 cut(s) 474, 527, 613, 631
FaqI GGGAC 1 cut(s) 489
FatI CATG 4 cut(s) 470, 523, 609, 627
Fnu4HI GCNGC 1 cut(s) 532
FokI GGATG 2 cut(s) 362, 398
Fsp4HI GCNGC 1 cut(s) 532
FspBI CTAG 3 cut(s) 131, 369, 594
GluI GCNGC 1 cut(s) 532
GsuI CTGGAG 2 cut(s) 231, 638
HaeIII GGCC 2 cut(s) 187, 673
HapII CCGG 2 cut(s) 568, 670
HgaI GACGC 1 cut(s) 143
Hin1II CATG 4 cut(s) 474, 527, 613, 631
HinfI GANTC 4 cut(s) 16, 45, 332, 477
HpaII CCGG 2 cut(s) 568, 670
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 3 cut(s) 7, 363, 412
Hpy188III TCNNGA 5 cut(s) 210, 218, 252, 329, 336
Hpy8I GTNNAC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 638
HpyCH4V TGCA 4 cut(s) 121, 523, 534, 647
HpyF3I CTNAG 2 cut(s) 450, 662
Hsp92II CATG 4 cut(s) 474, 527, 613, 631
Kzo9I GATC 4 cut(s) 7, 358, 589, 598
LpnPI CCDG 8 cut(s) 195, 201, 237, 251, 349, 466, 581, 668
Lsp1109I GCAGC 1 cut(s) 518
MaeI CTAG 3 cut(s) 131, 369, 594
MaeIII GTNAC 1 cut(s) 68
MalI GATC 4 cut(s) 9, 360, 591, 600
MboI GATC 4 cut(s) 7, 358, 589, 598
MboII GAAGA 4 cut(s) 92, 95, 314, 599
MflI RGATCY 2 cut(s) 358, 589
MluCI AATT 5 cut(s) 145, 203, 222, 269, 545
MlyI GAGTC 3 cut(s) 25, 54, 471
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 7 cut(s) 206, 228, 274, 382, 420, 431, 445
MroXI GAANNNNTTC 1 cut(s) 205
MseI TTAA 4 cut(s) 156, 273, 341, 506
MspI CCGG 2 cut(s) 568, 670
NdeII GATC 4 cut(s) 7, 358, 589, 598
NlaIII CATG 4 cut(s) 474, 527, 613, 631
NmuCI GTSAC 1 cut(s) 68
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 1 cut(s) 533
PleI GAGTC 3 cut(s) 24, 53, 471
PpsI GAGTC 3 cut(s) 24, 53, 471
PspPI GGNCC 1 cut(s) 185
PsuI RGATCY 2 cut(s) 358, 589
RsaI GTAC 2 cut(s) 105, 582
RsaNI GTAC 2 cut(s) 104, 581
SaqAI TTAA 4 cut(s) 156, 273, 341, 506
SatI GCNGC 1 cut(s) 532
Sau3AI GATC 4 cut(s) 7, 358, 589, 598
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 3 cut(s) 25, 54, 471
SetI ASST 7 cut(s) 30, 57, 109, 217, 285, 498, 533
SfcI CTRYAG 1 cut(s) 138
Sse9I AATT 5 cut(s) 145, 203, 222, 269, 545
SspMI CTAG 3 cut(s) 131, 369, 594
TaaI ACNGT 1 cut(s) 638
TaqI TCGA 1 cut(s) 310
TasI AATT 5 cut(s) 145, 203, 222, 269, 545
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 4 cut(s) 156, 273, 341, 506
Tru9I TTAA 4 cut(s) 156, 273, 341, 506
TscAI CASTG 1 cut(s) 643
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 1 cut(s) 531
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 4 cut(s) 314, 341, 410, 540
TspRI CASTG 1 cut(s) 643
XapI RAATTY 2 cut(s) 203, 269
XmnI GAANNNNTTC 1 cut(s) 205
XspI CTAG 3 cut(s) 131, 369, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.