RchiOBHm_Chr5g0057591

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000819
Physical Location & Seq
Forward (+)
61527854 .. 61534499
6646 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33436

Sequence Viewer

Length: 471 bp
ATGGCTGCTGAGTTTATGGTGAAGGACATGCAGTTAATTCGGGCAAAGAACAAATGGGGGACTCTGAGATTTCAAACCACTAACCTCTCTATTTTGATCAGTATCAGAAACTTTCTTTTGGATCAAGGGCTCAAGTGGACTGAAGATTGGTCCTTTGGTACTTGCTGGGAGTTGCACATCAAGGATTCCAGGTGGTATGGCGTTAAGCTTGTAAAGTGCCTTGTGCAAAATATTGTGGTTGATGTATCCTTCAATCAGTTAGGAGGGCTATGTACGTTATGCTTTCTTGAGCAGGTTGATCGCCTCATTGGCAAACATCATCTCTTTAAACGTAGTATCATACTGATTAAGGCATGGTGCTATTGTGAGAGTCGAATTCTCGGTGCCCATCACGGTTTGATATCTACATATGGTTTGGTGACCTTAGTTCTTTTTATCTACGTGTCATTGTTTCAGGTGAAAGGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

18.09

Weight (kDa)

9.1

Isoelectric Point (pI)

28.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP-OAS1 PF26180 103 - 148 1.3e-22 PAP/OAS1 substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 283
AccB1I GGYRCC 1 cut(s) 383
AclWI GGATC 1 cut(s) 129
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 2 cut(s) 160, 274
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 2 cut(s) 74, 253
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 1 cut(s) 208
AluI AGCT 1 cut(s) 208
AlwI GGATC 1 cut(s) 129
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 375
AspS9I GGNCC 1 cut(s) 150
AsuHPI GGTGA 3 cut(s) 31, 430, 469
AvaII GGWCC 1 cut(s) 150
BaeGI GKGCMC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 132
BccI CCATC 1 cut(s) 396
BcgI CGANNNNNNTGC 4 cut(s) 20, 54, 281, 315
BciT130I CCWGG 1 cut(s) 190
BciVI GTATCC 1 cut(s) 256
BclI TGATCA 1 cut(s) 96
BfuAI ACCTGC 1 cut(s) 283
BfuI GTATCC 1 cut(s) 256
BglI GCCNNNNNGGC 1 cut(s) 309
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 1 cut(s) 190
BpuEI CTTGAG 2 cut(s) 116, 308
BsaAI YACGTR 1 cut(s) 442
BsaBI GATNNNNATC 1 cut(s) 101
Bse8I GATNNNNATC 1 cut(s) 101
BseBI CCWGG 1 cut(s) 190
BseJI GATNNNNATC 1 cut(s) 101
BseMII CTCAG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 388
BseYI CCCAGC 1 cut(s) 165
BshNI GGYRCC 1 cut(s) 383
BslFI GGGAC 1 cut(s) 73
BsmFI GGGAC 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 132, 388
Bsp143I GATC 3 cut(s) 96, 121, 298
BspCNI CTCAG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 385
BspMI ACCTGC 1 cut(s) 283
BspPI GGATC 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 383
BssMI GATC 3 cut(s) 96, 121, 298
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 1 cut(s) 395
BstBAI YACGTR 1 cut(s) 442
BstDEI CTNAG 3 cut(s) 9, 65, 424
BstEII GGTNACC 1 cut(s) 418
BstKTI GATC 3 cut(s) 99, 124, 301
BstMBI GATC 3 cut(s) 96, 121, 298
BstMWI GCNNNNNNNGC 1 cut(s) 309
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 31
BstPI GGTNACC 1 cut(s) 418
BstSCI CCNGG 1 cut(s) 188
BstSLI GKGCMC 1 cut(s) 388
BsuI GTATCC 1 cut(s) 256
BveI ACCTGC 1 cut(s) 283
Cfr13I GGNCC 1 cut(s) 150
Csp6I GTAC 2 cut(s) 159, 273
CviAII CATG 2 cut(s) 28, 354
CviJI RGCY 4 cut(s) 5, 130, 208, 268
CviKI_1 RGCY 4 cut(s) 5, 130, 208, 268
CviQI GTAC 2 cut(s) 159, 273
DdeI CTNAG 3 cut(s) 9, 65, 424
DpnI GATC 3 cut(s) 98, 123, 300
DpnII GATC 3 cut(s) 96, 121, 298
DraI TTTAAA 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 162
Eco91I GGTNACC 1 cut(s) 418
EcoO65I GGTNACC 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 188
EcoRV GATATC 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 132
FaeI CATG 2 cut(s) 31, 357
FaiI YATR 9 cut(s) 17, 29, 198, 271, 280, 341, 355, 409, 411
FaqI GGGAC 1 cut(s) 73
FatI CATG 2 cut(s) 27, 353
FauNDI CATATG 1 cut(s) 409
FbaI TGATCA 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 6
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 1 cut(s) 169
Hin1II CATG 2 cut(s) 31, 357
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 3 cut(s) 61, 185, 370
HphI GGTGA 3 cut(s) 31, 430, 469
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 2 cut(s) 66, 107
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 1 cut(s) 138
HpyAV CCTTC 2 cut(s) 16, 259
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 3 cut(s) 275, 331, 441
HpyCH4V TGCA 3 cut(s) 31, 175, 226
HpyF10VI GCNNNNNNNGC 1 cut(s) 309
HpyF3I CTNAG 3 cut(s) 9, 65, 424
HpySE526I ACGT 3 cut(s) 275, 331, 441
Hsp92II CATG 2 cut(s) 31, 357
Ksp22I TGATCA 1 cut(s) 96
Kzo9I GATC 3 cut(s) 96, 121, 298
LpnPI CCDG 5 cut(s) 151, 175, 202, 278, 440
MaeII ACGT 3 cut(s) 275, 331, 441
MaeIII GTNAC 1 cut(s) 418
MalI GATC 3 cut(s) 98, 123, 300
MboI GATC 3 cut(s) 96, 121, 298
MboII GAAGA 1 cut(s) 155
MhlI GDGCHC 2 cut(s) 132, 388
MluCI AATT 2 cut(s) 36, 375
MlyI GAGTC 2 cut(s) 55, 379
MnlI CCTC 3 cut(s) 95, 257, 314
MseI TTAA 4 cut(s) 35, 204, 327, 348
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MwoI GCNNNNNNNGC 1 cut(s) 309
NdeI CATATG 1 cut(s) 409
NdeII GATC 3 cut(s) 96, 121, 298
NlaIII CATG 2 cut(s) 31, 357
NlaIV GGNNCC 1 cut(s) 385
NmuCI GTSAC 1 cut(s) 418
NspI RCATGY 1 cut(s) 31
PfeI GAWTC 1 cut(s) 185
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 55, 378
PpsI GAGTC 2 cut(s) 55, 378
Ppu21I YACGTR 1 cut(s) 442
Psp6I CCWGG 1 cut(s) 188
PspEI GGTNACC 1 cut(s) 418
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 385
PspPI GGNCC 1 cut(s) 150
RsaI GTAC 2 cut(s) 160, 274
RsaNI GTAC 2 cut(s) 159, 273
SaqAI TTAA 4 cut(s) 35, 204, 327, 348
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 96, 121, 298
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 2 cut(s) 55, 379
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 2 cut(s) 132, 388
SetI ASST 9 cut(s) 87, 194, 210, 278, 297, 334, 425, 444, 459
SinI GGWCC 1 cut(s) 150
SmlI CTYRAG 2 cut(s) 131, 287
SmoI CTYRAG 2 cut(s) 131, 287
Sse9I AATT 2 cut(s) 36, 375
SspI AATATT 1 cut(s) 232
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 3 cut(s) 278, 334, 444
TaqI TCGA 1 cut(s) 373
TasI AATT 2 cut(s) 36, 375
TfiI GAWTC 1 cut(s) 185
Tru1I TTAA 4 cut(s) 35, 204, 327, 348
Tru9I TTAA 4 cut(s) 35, 204, 327, 348
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 418
VpaK11BI GGWCC 1 cut(s) 150
XapI RAATTY 1 cut(s) 375
XceI RCATGY 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.