Rmu_sc0003522.1_g000035

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003522.1
OG0000819
Physical Location & Seq
Forward (+)
132606 .. 134760
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003522.1_g000035.1.cds

Sequence Viewer

Length: 438 bp
atgggcgatcttaggccttggtcgcttgagccaaacggcgccgttttggaggagaggccaacctcatcttcttcattcatgttttcaatttcaacaaatcagagggacgtttccgccgagtactggcggagagcggaggaggcaatgcagggggttatagctcagatccaatgtactcacgtctctgagcggagaaggaaagccgccattgattacgttcagaggatcattggagcttgtattggtgttcccgtttggatcagtacctctgaagacgtatctgtcggatggagtgctattcttgaaagggaagatcacaatatggctgttgagtttatggtgaaggacgtgcagttaattcgggcaaaggatcaagggctcaagtggactgaagattggtcctttggtacttgctgggagttgcacatcaaggattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

16.6

Weight (kDa)

5.1

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 38
AccBSI CCGCTC 2 cut(s) 134, 190
AciI CCGC 5 cut(s) 114, 127, 134, 190, 204
AclWI GGATC 4 cut(s) 160, 233, 266, 378
AcuI CTGAAG 2 cut(s) 291, 411
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 4 cut(s) 122, 175, 265, 409
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 3 cut(s) 87, 93, 305
AjiI CACGTC 2 cut(s) 181, 349
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 2 cut(s) 161, 236
AluI AGCT 2 cut(s) 161, 236
Alw26I GTCTC 1 cut(s) 187
AlwI GGATC 4 cut(s) 160, 233, 266, 378
AoxI GGCC 2 cut(s) 14, 56
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 1 cut(s) 399
AsuHPI GGTGA 1 cut(s) 352
AvaII GGWCC 1 cut(s) 399
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 279
BccI CCATC 1 cut(s) 282
BceAI ACGGC 2 cut(s) 26, 52
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BcoDI GTCTC 1 cut(s) 187
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 205
BmcAI AGTACT 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 399
BmgBI CACGTC 2 cut(s) 181, 349
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 1 cut(s) 40
BpiI GAAGAC 1 cut(s) 279
BpuEI CTTGAG 2 cut(s) 47, 365
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 17
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 17
BseGI GGATG 1 cut(s) 293
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 2 cut(s) 176, 177
BseNI ACTGG 1 cut(s) 128
BseRI GAGGAG 2 cut(s) 65, 152
BseYI CCCAGC 1 cut(s) 414
BsgI GTGCAG 1 cut(s) 371
BshFI GGCC 2 cut(s) 16, 58
BshNI GGYRCC 1 cut(s) 38
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 187
BsmBI CGTCTC 1 cut(s) 187
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 2 cut(s) 16, 58
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 6 cut(s) 7, 165, 225, 258, 313, 370
BspACI CCGC 5 cut(s) 114, 127, 134, 190, 204
BspANI GGCC 2 cut(s) 16, 58
BspCNI CTCAG 2 cut(s) 175, 178
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 4 cut(s) 160, 233, 266, 378
BspT107I GGYRCC 1 cut(s) 38
BsrBI CCGCTC 2 cut(s) 134, 190
BsrDI GCAATG 1 cut(s) 150
BsrI ACTGG 1 cut(s) 128
BssECI CCNNGG 1 cut(s) 17
BssMI GATC 6 cut(s) 7, 165, 225, 258, 313, 370
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 39
BstDEI CTNAG 3 cut(s) 11, 162, 186
BstF5I GGATG 1 cut(s) 293
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 6 cut(s) 10, 168, 228, 261, 316, 373
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 6 cut(s) 7, 165, 225, 258, 313, 370
BstMWI GCNNNNNNNGC 2 cut(s) 22, 140
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BsuRI GGCC 2 cut(s) 16, 58
BtrI CACGTC 2 cut(s) 181, 349
BtsCI GGATG 1 cut(s) 293
CfoI GCGC 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 399
Csp6I GTAC 4 cut(s) 121, 174, 264, 408
CviAII CATG 1 cut(s) 79
CviJI RGCY 8 cut(s) 16, 31, 58, 161, 203, 236, 326, 379
CviKI_1 RGCY 8 cut(s) 16, 31, 58, 161, 203, 236, 326, 379
CviQI GTAC 4 cut(s) 121, 174, 264, 408
DdeI CTNAG 3 cut(s) 11, 162, 186
DinI GGCGCC 1 cut(s) 40
DpnI GATC 6 cut(s) 9, 167, 227, 260, 315, 372
DpnII GATC 6 cut(s) 7, 165, 225, 258, 313, 370
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
EciI GGCGGA 2 cut(s) 103, 142
Eco130I CCWWGG 1 cut(s) 17
Eco147I AGGCCT 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 399
Eco57I CTGAAG 2 cut(s) 291, 411
EcoT14I CCWWGG 1 cut(s) 17
EcoT38I GRGCYC 1 cut(s) 381
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 17
Esp3I CGTCTC 1 cut(s) 187
FaeI CATG 1 cut(s) 82
FaiI YATR 4 cut(s) 80, 158, 323, 338
FaqI GGGAC 1 cut(s) 119
FatI CATG 1 cut(s) 78
Fnu4HI GCNGC 1 cut(s) 204
FokI GGATG 1 cut(s) 300
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 1 cut(s) 204
GlaI GCGC 1 cut(s) 40
GluI GCNGC 1 cut(s) 204
GsaI CCCAGC 1 cut(s) 418
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 2 cut(s) 16, 58
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 1 cut(s) 82
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 6 cut(s) 102, 165, 187, 222, 271, 287
Hpy188III TCNNGA 1 cut(s) 302
Hpy8I GTNNAC 1 cut(s) 387
HpyAV CCTTC 2 cut(s) 189, 337
HpyCH4IV ACGT 5 cut(s) 108, 180, 216, 276, 348
HpyCH4V TGCA 3 cut(s) 148, 352, 424
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 140
HpyF3I CTNAG 3 cut(s) 11, 162, 186
HpySE526I ACGT 5 cut(s) 108, 180, 216, 276, 348
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 1 cut(s) 82
HspAI GCGC 1 cut(s) 39
KasI GGCGCC 1 cut(s) 38
Kzo9I GATC 6 cut(s) 7, 165, 225, 258, 313, 370
LmnI GCTCC 1 cut(s) 233
LpnPI CCDG 3 cut(s) 109, 134, 400
MaeII ACGT 5 cut(s) 108, 180, 216, 276, 348
MalI GATC 6 cut(s) 9, 167, 227, 260, 315, 372
MbiI CCGCTC 2 cut(s) 134, 190
MboI GATC 6 cut(s) 7, 165, 225, 258, 313, 370
MboII GAAGA 5 cut(s) 60, 63, 284, 323, 404
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 2 cut(s) 87, 357
Mly113I GGCGCC 1 cut(s) 39
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 8 cut(s) 43, 48, 73, 96, 130, 133, 216, 277
MseI TTAA 1 cut(s) 356
MwoI GCNNNNNNNGC 2 cut(s) 22, 140
NarI GGCGCC 1 cut(s) 39
NdeII GATC 6 cut(s) 7, 165, 225, 258, 313, 370
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 1 cut(s) 40
NmeAIII GCCGAG 1 cut(s) 142
PceI AGGCCT 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PkrI GCNGC 1 cut(s) 205
PluTI GGCGCC 1 cut(s) 42
PspFI CCCAGC 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 1 cut(s) 399
PsuI RGATCY 1 cut(s) 165
RsaI GTAC 4 cut(s) 122, 175, 265, 409
RsaNI GTAC 4 cut(s) 121, 174, 264, 408
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 1 cut(s) 204
Sau3AI GATC 6 cut(s) 7, 165, 225, 258, 313, 370
Sau96I GGNCC 1 cut(s) 399
ScaI AGTACT 1 cut(s) 122
SduI GDGCHC 1 cut(s) 381
SetI ASST 9 cut(s) 65, 111, 163, 183, 219, 238, 269, 279, 351
SfoI GGCGCC 1 cut(s) 40
SinI GGWCC 1 cut(s) 399
SmlI CTYRAG 2 cut(s) 26, 380
SmoI CTYRAG 2 cut(s) 26, 380
Sse9I AATT 2 cut(s) 87, 357
SseBI AGGCCT 1 cut(s) 16
SsiI CCGC 5 cut(s) 114, 127, 134, 190, 204
SspDI GGCGCC 1 cut(s) 38
StuI AGGCCT 1 cut(s) 16
StyI CCWWGG 1 cut(s) 17
TaiI ACGT 5 cut(s) 111, 183, 219, 279, 351
TasI AATT 2 cut(s) 87, 357
TatI WGTACW 2 cut(s) 120, 173
TauI GCSGC 1 cut(s) 206
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TspDTI ATGAA 2 cut(s) 63, 67
VpaK11BI GGWCC 1 cut(s) 399
ZrmI AGTACT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.