Rh5AG374400

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000819
Physical Location & Seq
Reverse (-)
63628263 .. 63640083
11821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG374400.1

Sequence Viewer

Length: 384 bp
ATGTTCGACGAGCTATTAGTCATCTTCCTTATCCTGGTCGTCAACGTGATCGGCAGAATATGGCAGGAGAGCAACACCGAGATGCCACTTGAGGCCTTAAAGGAGATTCAGTCGCAACACCAACGATGGTTAGAGACAGGAAGAAGGCCTTGGTCGCTTGAGCCAAACGGCGCCGTTTTGGAGGAGAGGCCGACCTCATCTTCTTCATCCGTGTTTTCAATTTCAACAAATCAGAAGGACATTTCCGCCGAGTACTGGCGGAGAGCGGAGGAGGCAATGCAGGGGGTTATAGCTCAGATCCAATGTACTCACGTCTCTGAGCGGAGAAGGAAAGCTGCCATTGATTACGTTCAGCGGATCATTGGAGCTTGTATTGGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

14.55

Weight (kDa)

5.71

Isoelectric Point (pI)

65.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AccBSI CCGCTC 2 cut(s) 266, 322
AciI CCGC 5 cut(s) 246, 259, 266, 322, 355
AclWI GGATC 2 cut(s) 292, 365
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 2 cut(s) 254, 307
AfiI CCNNNNNNNGG 2 cut(s) 34, 255
AgsI TTSAA 2 cut(s) 219, 225
AjiI CACGTC 1 cut(s) 313
AjnI CCWGG 1 cut(s) 33
AjuI GAANNNNNNNTTGG 2 cut(s) 133, 165
AluBI AGCT 4 cut(s) 13, 293, 335, 368
AluI AGCT 4 cut(s) 13, 293, 335, 368
Alw26I GTCTC 2 cut(s) 128, 319
AlwI GGATC 2 cut(s) 292, 365
AoxI GGCC 3 cut(s) 93, 146, 188
ApeKI GCWGC 1 cut(s) 335
AspLEI GCGC 1 cut(s) 173
BanI GGYRCC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 120
BceAI ACGGC 2 cut(s) 158, 184
BciT130I CCWGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 128, 319
BfoI RGCGCY 1 cut(s) 174
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 1 cut(s) 35
BmgBI CACGTC 1 cut(s) 313
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 1 cut(s) 35
BmsI GCATC 1 cut(s) 72
BpuEI CTTGAG 2 cut(s) 110, 179
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 255
Bse1I ACTGG 1 cut(s) 260
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 1 cut(s) 206
BseLI CCNNNNNNNGG 2 cut(s) 34, 255
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 2 cut(s) 308, 309
BseNI ACTGG 1 cut(s) 260
BseRI GAGGAG 2 cut(s) 197, 284
BseXI GCAGC 1 cut(s) 322
BshFI GGCC 3 cut(s) 95, 148, 190
BshNI GGYRCC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 34, 255
BsmAI GTCTC 2 cut(s) 128, 319
BsmBI CGTCTC 1 cut(s) 319
BsnI GGCC 3 cut(s) 95, 148, 190
Bsp143I GATC 3 cut(s) 48, 297, 357
BspACI CCGC 5 cut(s) 246, 259, 266, 322, 355
BspANI GGCC 3 cut(s) 95, 148, 190
BspCNI CTCAG 2 cut(s) 307, 310
BspLI GGNNCC 1 cut(s) 172
BspPI GGATC 2 cut(s) 292, 365
BspT107I GGYRCC 1 cut(s) 170
BsrBI CCGCTC 2 cut(s) 266, 322
BsrDI GCAATG 1 cut(s) 282
BsrI ACTGG 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 3 cut(s) 48, 297, 357
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 294, 318
BstF5I GGATG 1 cut(s) 206
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 3 cut(s) 51, 300, 360
BstMAI GTCTC 2 cut(s) 128, 319
BstMBI GATC 3 cut(s) 48, 297, 357
BstMWI GCNNNNNNNGC 2 cut(s) 154, 272
BstNI CCWGG 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 322
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
BsuRI GGCC 3 cut(s) 95, 148, 190
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 1 cut(s) 206
CfoI GCGC 1 cut(s) 173
Csp6I GTAC 2 cut(s) 253, 306
CviJI RGCY 8 cut(s) 13, 95, 148, 163, 190, 293, 335, 368
CviKI_1 RGCY 8 cut(s) 13, 95, 148, 163, 190, 293, 335, 368
CviQI GTAC 2 cut(s) 253, 306
DdeI CTNAG 2 cut(s) 294, 318
DinI GGCGCC 1 cut(s) 172
DpnI GATC 3 cut(s) 50, 299, 359
DpnII GATC 3 cut(s) 48, 297, 357
EciI GGCGGA 2 cut(s) 235, 274
Eco130I CCWWGG 1 cut(s) 149
Eco147I AGGCCT 2 cut(s) 95, 148
EcoRII CCWGG 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 149
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 319
FaiI YATR 2 cut(s) 61, 290
FalI AAGNNNNNCTT 2 cut(s) 133, 165
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 1 cut(s) 193
Fsp4HI GCNGC 1 cut(s) 336
GlaI GCGC 1 cut(s) 172
GluI GCNGC 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 3 cut(s) 95, 148, 190
HhaI GCGC 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 171
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 3 cut(s) 234, 297, 319
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 3 cut(s) 138, 229, 321
HpyCH4IV ACGT 3 cut(s) 45, 312, 348
HpyCH4V TGCA 1 cut(s) 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 272
HpyF3I CTNAG 2 cut(s) 294, 318
HpySE526I ACGT 3 cut(s) 45, 312, 348
Hsp92I GRCGYC 1 cut(s) 171
HspAI GCGC 1 cut(s) 171
KasI GGCGCC 1 cut(s) 170
Kzo9I GATC 3 cut(s) 48, 297, 357
LmnI GCTCC 1 cut(s) 365
LpnPI CCDG 6 cut(s) 20, 47, 50, 123, 241, 266
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 72
MaeII ACGT 3 cut(s) 45, 312, 348
MalI GATC 3 cut(s) 50, 299, 359
MbiI CCGCTC 2 cut(s) 266, 322
MboI GATC 3 cut(s) 48, 297, 357
MboII GAAGA 4 cut(s) 16, 153, 192, 195
MflI RGATCY 1 cut(s) 297
MluCI AATT 1 cut(s) 219
Mly113I GGCGCC 1 cut(s) 171
MnlI CCTC 6 cut(s) 85, 175, 180, 205, 262, 265
MseI TTAA 1 cut(s) 98
MslI CAYNNNNRTG 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 355
MspR9I CCNGG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 154, 272
NarI GGCGCC 1 cut(s) 171
NdeII GATC 3 cut(s) 48, 297, 357
NlaIV GGNNCC 1 cut(s) 172
NmeAIII GCCGAG 1 cut(s) 274
PceI AGGCCT 2 cut(s) 95, 148
PfeI GAWTC 1 cut(s) 106
PkrI GCNGC 1 cut(s) 337
PluTI GGCGCC 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 172
PsuI RGATCY 1 cut(s) 297
RsaI GTAC 2 cut(s) 254, 307
RsaNI GTAC 2 cut(s) 253, 306
RseI CAYNNNNRTG 1 cut(s) 80
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 3 cut(s) 48, 297, 357
ScaI AGTACT 1 cut(s) 254
ScrFI CCNGG 1 cut(s) 35
SetI ASST 8 cut(s) 15, 48, 197, 295, 315, 337, 351, 370
SfaNI GCATC 1 cut(s) 72
SfoI GGCGCC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 2 cut(s) 89, 158
SmoI CTYRAG 2 cut(s) 89, 158
Sse9I AATT 1 cut(s) 219
SseBI AGGCCT 2 cut(s) 95, 148
SsiI CCGC 5 cut(s) 246, 259, 266, 322, 355
SspDI GGCGCC 1 cut(s) 170
StuI AGGCCT 2 cut(s) 95, 148
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 149
TaiI ACGT 3 cut(s) 48, 315, 351
TaqI TCGA 1 cut(s) 6
TasI AATT 1 cut(s) 219
TatI WGTACW 2 cut(s) 252, 305
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 195
TspGWI ACGGA 1 cut(s) 199
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.