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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 532, 830 |
| AclWI | GGATC | 4 cut(s) | 915, 1038, 1167, 1214 |
| AcsI | RAATTY | 3 cut(s) | 712, 1021, 1271 |
| AcuI | CTGAAG | 1 cut(s) | 942 |
| AfaI | GTAC | 2 cut(s) | 292, 1205 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 123, 531 |
| AgsI | TTSAA | 3 cut(s) | 305, 986, 1171 |
| AjnI | CCWGG | 1 cut(s) | 331 |
| Alw26I | GTCTC | 4 cut(s) | 149, 186, 356, 623 |
| AlwI | GGATC | 4 cut(s) | 915, 1038, 1167, 1214 |
| Ama87I | CYCGRG | 1 cut(s) | 106 |
| AoxI | GGCC | 2 cut(s) | 1011, 1101 |
| ApoI | RAATTY | 3 cut(s) | 712, 1021, 1271 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 449, 481, 839, 871 |
| AseI | ATTAAT | 1 cut(s) | 263 |
| AsuC2I | CCSGG | 1 cut(s) | 39 |
| AsuII | TTCGAA | 1 cut(s) | 657 |
| AvaI | CYCGRG | 1 cut(s) | 106 |
| AxyI | CCTNAGG | 1 cut(s) | 118 |
| BaeGI | GKGCMC | 1 cut(s) | 181 |
| BauI | CACGAG | 1 cut(s) | 180 |
| BbsI | GAAGAC | 2 cut(s) | 678, 1251 |
| BccI | CCATC | 5 cut(s) | 117, 487, 721, 827, 908 |
| BceAI | ACGGC | 1 cut(s) | 648 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1112, 1146 |
| BciT130I | CCWGG | 1 cut(s) | 333 |
| BclI | TGATCA | 1 cut(s) | 406 |
| BcnI | CCSGG | 1 cut(s) | 39 |
| BcoDI | GTCTC | 4 cut(s) | 149, 186, 356, 623 |
| BglII | AGATCT | 1 cut(s) | 570 |
| BmcAI | AGTACT | 1 cut(s) | 292 |
| Bme1390I | CCNGG | 2 cut(s) | 39, 333 |
| BmeT110I | CYCGRG | 1 cut(s) | 106 |
| BmrFI | CCNGG | 2 cut(s) | 39, 333 |
| BmsI | GCATC | 5 cut(s) | 150, 574, 744, 814, 1006 |
| BpiI | GAAGAC | 2 cut(s) | 678, 1251 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 340, 372 |
| BpmI | CTGGAG | 1 cut(s) | 315 |
| Bpu14I | TTCGAA | 1 cut(s) | 657 |
| BpuMI | CCSGG | 1 cut(s) | 39 |
| BsaJI | CCNNGG | 2 cut(s) | 38, 332 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 768, 798 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 123, 531 |
| Bse1I | ACTGG | 1 cut(s) | 868 |
| Bse21I | CCTNAGG | 1 cut(s) | 118 |
| Bse3DI | GCAATG | 1 cut(s) | 330 |
| BseBI | CCWGG | 1 cut(s) | 333 |
| BseDI | CCNNGG | 2 cut(s) | 38, 332 |
| BseGI | GGATG | 7 cut(s) | 128, 257, 589, 736, 759, 829, 838 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 123, 531 |
| BseMI | GCAATG | 1 cut(s) | 330 |
| BseMII | CTCAG | 5 cut(s) | 801, 960, 999, 1089, 1128 |
| BseNI | ACTGG | 1 cut(s) | 868 |
| BseRI | GAGGAG | 2 cut(s) | 301, 942 |
| BseSI | GKGCMC | 1 cut(s) | 181 |
| BsgI | GTGCAG | 1 cut(s) | 576 |
| BshFI | GGCC | 2 cut(s) | 1013, 1103 |
| BsiHKCI | CYCGRG | 1 cut(s) | 106 |
| BsiSI | CCGG | 2 cut(s) | 38, 1223 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 123, 531 |
| BsmAI | GTCTC | 4 cut(s) | 149, 186, 356, 623 |
| BsmBI | CGTCTC | 1 cut(s) | 623 |
| BsnI | GGCC | 2 cut(s) | 1013, 1103 |
| BsoBI | CYCGRG | 1 cut(s) | 106 |
| Bsp119I | TTCGAA | 1 cut(s) | 657 |
| Bsp1286I | GDGCHC | 1 cut(s) | 181 |
| Bsp143I | GATC | 7 cut(s) | 406, 570, 907, 937, 1030, 1159, 1219 |
| BspACI | CCGC | 2 cut(s) | 532, 830 |
| BspANI | GGCC | 2 cut(s) | 1013, 1103 |
| BspCNI | CTCAG | 5 cut(s) | 802, 961, 1000, 1090, 1129 |
| BspPI | GGATC | 4 cut(s) | 915, 1038, 1167, 1214 |
| BspT104I | TTCGAA | 1 cut(s) | 657 |
| BsrDI | GCAATG | 1 cut(s) | 330 |
| BsrI | ACTGG | 1 cut(s) | 868 |
| BssECI | CCNNGG | 2 cut(s) | 38, 332 |
| BssMI | GATC | 7 cut(s) | 406, 570, 907, 937, 1030, 1159, 1219 |
| BssSI | CACGAG | 1 cut(s) | 180 |
| Bst2BI | CACGAG | 1 cut(s) | 180 |
| Bst2UI | CCWGG | 1 cut(s) | 333 |
| Bst4CI | ACNGT | 1 cut(s) | 96 |
| Bst6I | CTCTTC | 4 cut(s) | 158, 813, 1050, 1179 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 382 |
| BstBI | TTCGAA | 1 cut(s) | 657 |
| BstC8I | GCNNGC | 3 cut(s) | 103, 216, 489 |
| BstDEI | CTNAG | 6 cut(s) | 118, 810, 969, 1008, 1098, 1137 |
| BstF5I | GGATG | 7 cut(s) | 128, 257, 589, 736, 759, 829, 838 |
| BstKTI | GATC | 7 cut(s) | 409, 573, 910, 940, 1033, 1162, 1222 |
| BstMAI | GTCTC | 4 cut(s) | 149, 186, 356, 623 |
| BstMBI | GATC | 7 cut(s) | 406, 570, 907, 937, 1030, 1159, 1219 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 73, 382, 441, 497, 506, 1010, 1100, 1139, 1284 |
| BstNI | CCWGG | 1 cut(s) | 333 |
| BstNSI | RCATGY | 1 cut(s) | 491 |
| BstSCI | CCNGG | 2 cut(s) | 37, 331 |
| BstSLI | GKGCMC | 1 cut(s) | 181 |
| BstV2I | GAAGAC | 2 cut(s) | 678, 1251 |
| BstX2I | RGATCY | 1 cut(s) | 570 |
| BstYI | RGATCY | 1 cut(s) | 570 |
| Bsu36I | CCTNAGG | 1 cut(s) | 118 |
| BsuRI | GGCC | 2 cut(s) | 1013, 1103 |
| BtsCI | GGATG | 7 cut(s) | 128, 257, 589, 736, 759, 829, 838 |
| BtsIMutI | CAGTG | 2 cut(s) | 253, 861 |
| Cac8I | GCNNGC | 3 cut(s) | 103, 216, 489 |
| CseI | GACGC | 1 cut(s) | 328 |
| Csp6I | GTAC | 2 cut(s) | 291, 1204 |
| CviAII | CATG | 7 cut(s) | 55, 410, 440, 488, 668, 676, 709 |
| CviQI | GTAC | 2 cut(s) | 291, 1204 |
| DdeI | CTNAG | 6 cut(s) | 118, 810, 969, 1008, 1098, 1137 |
| DpnI | GATC | 7 cut(s) | 408, 572, 909, 939, 1032, 1161, 1221 |
| DpnII | GATC | 7 cut(s) | 406, 570, 907, 937, 1030, 1159, 1219 |
| Eam1104I | CTCTTC | 4 cut(s) | 158, 813, 1050, 1179 |
| EarI | CTCTTC | 4 cut(s) | 158, 813, 1050, 1179 |
| Eco32I | GATATC | 1 cut(s) | 279 |
| Eco57I | CTGAAG | 1 cut(s) | 942 |
| Eco81I | CCTNAGG | 1 cut(s) | 118 |
| Eco88I | CYCGRG | 1 cut(s) | 106 |
| EcoRII | CCWGG | 1 cut(s) | 331 |
| EcoRV | GATATC | 1 cut(s) | 279 |
| EcoT22I | ATGCAT | 1 cut(s) | 143 |
| Esp3I | CGTCTC | 1 cut(s) | 623 |
| FaeI | CATG | 7 cut(s) | 58, 413, 443, 491, 671, 679, 712 |
| FatI | CATG | 7 cut(s) | 54, 409, 439, 487, 667, 675, 708 |
| FauI | CCCGC | 1 cut(s) | 525 |
| FbaI | TGATCA | 1 cut(s) | 406 |
| FokI | GGATG | 7 cut(s) | 135, 264, 596, 743, 766, 836, 845 |
| GsuI | CTGGAG | 1 cut(s) | 315 |
| HaeIII | GGCC | 2 cut(s) | 1013, 1103 |
| HapII | CCGG | 2 cut(s) | 38, 1223 |
| HgaI | GACGC | 1 cut(s) | 328 |
| Hin1II | CATG | 7 cut(s) | 58, 413, 443, 491, 671, 679, 712 |
| HincII | GTYRAC | 1 cut(s) | 114 |
| HindII | GTYRAC | 1 cut(s) | 114 |
| HinfI | GANTC | 3 cut(s) | 328, 349, 644 |
| HpaII | CCGG | 2 cut(s) | 38, 1223 |
| Hpy166II | GTNNAC | 1 cut(s) | 114 |
| Hpy188I | TCNGA | 2 cut(s) | 283, 649 |
| Hpy188III | TCNNGA | 5 cut(s) | 133, 574, 905, 911, 1168 |
| Hpy8I | GTNNAC | 1 cut(s) | 114 |
| Hpy99I | CGWCG | 1 cut(s) | 461 |
| HpyAV | CCTTC | 4 cut(s) | 644, 743, 917, 1075 |
| HpyCH4III | ACNGT | 1 cut(s) | 96 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 73, 382, 441, 497, 506, 1010, 1100, 1139, 1284 |
| HpyF3I | CTNAG | 6 cut(s) | 118, 810, 969, 1008, 1098, 1137 |
| Hsp92II | CATG | 7 cut(s) | 58, 413, 443, 491, 671, 679, 712 |
| Ksp22I | TGATCA | 1 cut(s) | 406 |
| Kzo9I | GATC | 7 cut(s) | 406, 570, 907, 937, 1030, 1159, 1219 |
| LmnI | GCTCC | 2 cut(s) | 40, 126 |
| LweI | GCATC | 5 cut(s) | 150, 574, 744, 814, 1006 |
| MaeIII | GTNAC | 3 cut(s) | 256, 557, 1259 |
| MalI | GATC | 7 cut(s) | 408, 572, 909, 939, 1032, 1161, 1221 |
| MboI | GATC | 7 cut(s) | 406, 570, 907, 937, 1030, 1159, 1219 |
| MboII | GAAGA | 8 cut(s) | 175, 671, 683, 827, 830, 1067, 1196, 1256 |
| MflI | RGATCY | 1 cut(s) | 570 |
| MhlI | GDGCHC | 1 cut(s) | 181 |
| MluCI | AATT | 7 cut(s) | 172, 306, 359, 371, 712, 1021, 1271 |
| MlyI | GAGTC | 2 cut(s) | 322, 358 |
| MmeI | TCCRAC | 1 cut(s) | 1233 |
| Mph1103I | ATGCAT | 1 cut(s) | 143 |
| MseI | TTAA | 4 cut(s) | 23, 263, 309, 683 |
| MspA1I | CMGCKG | 1 cut(s) | 599 |
| MspI | CCGG | 2 cut(s) | 38, 1223 |
| MspR9I | CCNGG | 2 cut(s) | 39, 333 |
| MvaI | CCWGG | 1 cut(s) | 333 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 73, 382, 441, 497, 506, 1010, 1100, 1139, 1284 |
| NciI | CCSGG | 1 cut(s) | 39 |
| NdeII | GATC | 7 cut(s) | 406, 570, 907, 937, 1030, 1159, 1219 |
| NlaIII | CATG | 7 cut(s) | 58, 413, 443, 491, 671, 679, 712 |
| NmuCI | GTSAC | 1 cut(s) | 1259 |
| NsiI | ATGCAT | 1 cut(s) | 143 |
| NspI | RCATGY | 1 cut(s) | 491 |
| NspV | TTCGAA | 1 cut(s) | 657 |
| PaeI | GCATGC | 1 cut(s) | 491 |
| PfeI | GAWTC | 1 cut(s) | 644 |
| PleI | GAGTC | 2 cut(s) | 322, 357 |
| PpsI | GAGTC | 2 cut(s) | 322, 357 |
| PshBI | ATTAAT | 1 cut(s) | 263 |
| Psp6I | CCWGG | 1 cut(s) | 331 |
| PspGI | CCWGG | 1 cut(s) | 331 |
| PsuI | RGATCY | 1 cut(s) | 570 |
| PvuII | CAGCTG | 1 cut(s) | 599 |
| RsaI | GTAC | 2 cut(s) | 292, 1205 |
| RsaNI | GTAC | 2 cut(s) | 291, 1204 |
| SaqAI | TTAA | 4 cut(s) | 23, 263, 309, 683 |
| Sau3AI | GATC | 7 cut(s) | 406, 570, 907, 937, 1030, 1159, 1219 |
| ScaI | AGTACT | 1 cut(s) | 292 |
| SchI | GAGTC | 2 cut(s) | 322, 358 |
| ScrFI | CCNGG | 2 cut(s) | 39, 333 |
| SduI | GDGCHC | 1 cut(s) | 181 |
| SfaNI | GCATC | 5 cut(s) | 150, 574, 744, 814, 1006 |
| SfuI | TTCGAA | 1 cut(s) | 657 |
| SphI | GCATGC | 1 cut(s) | 491 |
| Sse9I | AATT | 7 cut(s) | 172, 306, 359, 371, 712, 1021, 1271 |
| SsiI | CCGC | 2 cut(s) | 532, 830 |
| SspI | AATATT | 2 cut(s) | 1048, 1153 |
| StyD4I | CCNGG | 2 cut(s) | 37, 331 |
| TaaI | ACNGT | 1 cut(s) | 96 |
| TaqI | TCGA | 6 cut(s) | 575, 657, 858, 910, 975, 1233 |
| TasI | AATT | 7 cut(s) | 172, 306, 359, 371, 712, 1021, 1271 |
| TatI | WGTACW | 2 cut(s) | 290, 1203 |
| TfiI | GAWTC | 1 cut(s) | 644 |
| Tru1I | TTAA | 4 cut(s) | 23, 263, 309, 683 |
| Tru9I | TTAA | 4 cut(s) | 23, 263, 309, 683 |
| TscAI | CASTG | 2 cut(s) | 253, 868 |
| TseFI | GTSAC | 1 cut(s) | 1259 |
| Tsp45I | GTSAC | 1 cut(s) | 1259 |
| TspGWI | ACGGA | 1 cut(s) | 1172 |
| TspRI | CASTG | 2 cut(s) | 253, 868 |
| VspI | ATTAAT | 1 cut(s) | 263 |
| XapI | RAATTY | 3 cut(s) | 712, 1021, 1271 |
| XceI | RCATGY | 1 cut(s) | 491 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 532 |
| ZrmI | AGTACT | 1 cut(s) | 292 |
| Zsp2I | ATGCAT | 1 cut(s) | 143 |