RLG00000036011

3-dehydroquinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001467
Physical Location & Seq
Reverse (-)
74847875 .. 74850184
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036011

Sequence Viewer

Length: 729 bp
ATGGCGAAGCAATCTAAAGCCAAGAAATCGGAGAATTTGGGAAAGTGTACCGTCACCCCCGTCCAGATCGCCTTCCTCGTCAGCCAGTTTCTCTTTGATAACAGCTACACCGATTCTGAACTGAAGCCTCCTCACTCTTCTCTACGTCTCCGATTCAAGAGATTGATTTCTTCACATAATAAACACCCAATGGAAACCAAGGCCACCCAGAGTTCATCAGTTGCTTCTTCTACGACATCGTTTGGGAGCTCGAAGAAGATGGTGTGGGTTTGGACGGAGAGCAAGCAGGTCATGACGGCCGCCGTGGAGAGAGGCTGGAACACCTTCCTGTTTCAGAGTCAGAAACTCGCCGATGACTGGTCTTCGATTGCGTTGATAGATCCTCTTTTGATCAAAGAGAGAGGGATTTTCGATAGCGAGAACACGAGAGTTGCCACAGTTGTTGAGGTTTCCAATCCCAAAGAGTTGGAAGAGCTTCAACCCAAAAACGGGGTGGCTGAGAATGTTGTTGTTGATTTGCTGGATTGGCAGGCAGGTGAGCTTATGAAATTTGAGTACCAAAAGAAGGTCGCCTCGCTGAGTAAGCTAAAGAAATGTAGTACTAACTCAGAAGCATTGGAGAAATCAAGAGCAGCAGTGAGTCATCTGCATATAAGATACATAGTTGACATGCAATCCATGGACCCAACAGTATCAAAGATAAACTCTTTTACTTTCAGCCTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHQS

Protein Analysis

243

Amino Acids

27.21

Weight (kDa)

9.21

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHQS PF01959 85 - 177 3.1e-17 3-dehydroquinate synthase II N-terminal domain
DUF632 PF04782 178 - 236 9.9e-12 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 524
Acc36I ACCTGC 2 cut(s) 277, 524
AciI CCGC 1 cut(s) 300
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 2 cut(s) 34, 548
AcuI CTGAAG 1 cut(s) 143
AfaI GTAC 3 cut(s) 49, 557, 601
AfiI CCNNNNNNNGG 4 cut(s) 357, 488, 489, 565
AgsI TTSAA 2 cut(s) 157, 479
AluBI AGCT 5 cut(s) 105, 249, 475, 541, 586
AluI AGCT 5 cut(s) 105, 249, 475, 541, 586
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 1 cut(s) 374
AoxI GGCC 2 cut(s) 201, 297
ApeKI GCWGC 1 cut(s) 632
ApoI RAATTY 2 cut(s) 34, 548
Asp700I GAANNNNTTC 2 cut(s) 323, 474
AspS9I GGNCC 1 cut(s) 682
AsuHPI GGTGA 2 cut(s) 46, 548
AvaII GGWCC 1 cut(s) 682
BanII GRGCYC 1 cut(s) 251
BauI CACGAG 1 cut(s) 424
BbsI GAAGAC 1 cut(s) 354
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 1 cut(s) 644
BccI CCATC 1 cut(s) 253
BceAI ACGGC 2 cut(s) 287, 312
BclI TGATCA 1 cut(s) 390
BcoDI GTCTC 1 cut(s) 152
BfuAI ACCTGC 2 cut(s) 277, 524
BisI GCNGC 2 cut(s) 300, 633
BlsI GCNGC 2 cut(s) 301, 634
BmcAI AGTACT 1 cut(s) 601
Bme18I GGWCC 1 cut(s) 682
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 1 cut(s) 684
BpiI GAAGAC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 198, 303, 678
Bsc4I CCNNNNNNNGG 4 cut(s) 357, 488, 489, 565
Bse1I ACTGG 2 cut(s) 85, 362
BseDI CCNNGG 3 cut(s) 198, 303, 678
BseLI CCNNNNNNNGG 4 cut(s) 357, 488, 489, 565
BseMII CTCAG 3 cut(s) 489, 569, 621
BseNI ACTGG 2 cut(s) 85, 362
BseRI GAGGAG 1 cut(s) 120
BseX3I CGGCCG 1 cut(s) 297
BseXI GCAGC 1 cut(s) 644
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 2 cut(s) 203, 299
BsiEI CGRYCG 1 cut(s) 300
BsiHKAI GWGCWC 1 cut(s) 251
BslI CCNNNNNNNGG 4 cut(s) 357, 488, 489, 565
BsmAI GTCTC 1 cut(s) 152
BsmBI CGTCTC 1 cut(s) 152
BsnI GGCC 2 cut(s) 203, 299
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 3 cut(s) 66, 379, 390
Bsp19I CCATGG 1 cut(s) 678
BspACI CCGC 1 cut(s) 300
BspANI GGCC 2 cut(s) 203, 299
BspCNI CTCAG 3 cut(s) 490, 570, 620
BspHI TCATGA 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 684
BspMI ACCTGC 2 cut(s) 277, 524
BspPI GGATC 1 cut(s) 374
BspQI GCTCTTC 1 cut(s) 465
BsrI ACTGG 2 cut(s) 85, 362
BssECI CCNNGG 3 cut(s) 198, 303, 678
BssMI GATC 3 cut(s) 66, 379, 390
BssSI CACGAG 1 cut(s) 424
BssT1I CCWWGG 2 cut(s) 198, 678
Bst2BI CACGAG 1 cut(s) 424
Bst4CI ACNGT 3 cut(s) 52, 439, 691
Bst6I CTCTTC 2 cut(s) 142, 465
BstC8I GCNNGC 2 cut(s) 284, 531
BstDEI CTNAG 3 cut(s) 498, 578, 607
BstDSI CCRYGG 2 cut(s) 303, 678
BstKTI GATC 3 cut(s) 69, 382, 393
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 3 cut(s) 66, 379, 390
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 2 cut(s) 526, 583
BstNSI RCATGY 1 cut(s) 673
BstV1I GCAGC 1 cut(s) 644
BstV2I GAAGAC 1 cut(s) 354
BstX2I RGATCY 1 cut(s) 379
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 1 cut(s) 379
BstZI CGGCCG 1 cut(s) 297
BsuRI GGCC 2 cut(s) 203, 299
BtgI CCRYGG 2 cut(s) 303, 678
BtsI GCAGTG 1 cut(s) 642
BtsIMutI CAGTG 1 cut(s) 642
BveI ACCTGC 2 cut(s) 277, 524
Cac8I GCNNGC 2 cut(s) 284, 531
CciI TCATGA 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 682
Csp6I GTAC 3 cut(s) 48, 556, 600
CspCI CAANNNNNGTGG 2 cut(s) 424, 459
CviAII CATG 3 cut(s) 292, 670, 679
CviQI GTAC 3 cut(s) 48, 556, 600
DdeI CTNAG 3 cut(s) 498, 578, 607
DpnI GATC 3 cut(s) 68, 381, 392
DpnII GATC 3 cut(s) 66, 379, 390
EaeI YGGCCR 1 cut(s) 297
EagI CGGCCG 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 142, 465
EarI CTCTTC 2 cut(s) 142, 465
Ecl136II GAGCTC 1 cut(s) 249
EclXI CGGCCG 1 cut(s) 297
Eco130I CCWWGG 2 cut(s) 198, 678
Eco24I GRGCYC 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 682
Eco52I CGGCCG 1 cut(s) 297
Eco53kI GAGCTC 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 249
EcoT14I CCWWGG 2 cut(s) 198, 678
EcoT38I GRGCYC 1 cut(s) 251
ErhI CCWWGG 2 cut(s) 198, 678
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 3 cut(s) 295, 673, 682
FaiI YATR 8 cut(s) 177, 293, 545, 651, 653, 662, 671, 680
FatI CATG 3 cut(s) 291, 669, 678
FbaI TGATCA 1 cut(s) 390
Fnu4HI GCNGC 2 cut(s) 300, 633
FriOI GRGCYC 1 cut(s) 251
Fsp4HI GCNGC 2 cut(s) 300, 633
GluI GCNGC 2 cut(s) 300, 633
HaeIII GGCC 2 cut(s) 203, 299
Hin1II CATG 3 cut(s) 295, 673, 682
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 4 cut(s) 113, 153, 337, 640
HphI GGTGA 2 cut(s) 46, 548
Hpy166II GTNNAC 2 cut(s) 48, 667
Hpy188I TCNGA 6 cut(s) 31, 118, 152, 336, 342, 610
Hpy188III TCNNGA 4 cut(s) 64, 157, 292, 627
Hpy8I GTNNAC 2 cut(s) 48, 667
HpyAV CCTTC 3 cut(s) 82, 334, 559
HpyCH4III ACNGT 3 cut(s) 52, 439, 691
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 2 cut(s) 649, 673
HpyF10VI GCNNNNNNNGC 2 cut(s) 526, 583
HpyF3I CTNAG 3 cut(s) 498, 578, 607
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 3 cut(s) 295, 673, 682
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 3 cut(s) 66, 379, 390
LguI GCTCTTC 1 cut(s) 465
LmnI GCTCC 1 cut(s) 246
Lsp1109I GCAGC 1 cut(s) 644
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 1 cut(s) 52
MalI GATC 3 cut(s) 68, 381, 392
MboI GATC 3 cut(s) 66, 379, 390
MboII GAAGA 7 cut(s) 129, 162, 219, 265, 268, 354, 482
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 2 cut(s) 34, 548
MlyI GAGTC 2 cut(s) 346, 649
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 8 cut(s) 86, 138, 141, 305, 393, 395, 439, 583
MroXI GAANNNNTTC 2 cut(s) 323, 474
MwoI GCNNNNNNNGC 2 cut(s) 526, 583
NcoI CCATGG 1 cut(s) 678
NdeII GATC 3 cut(s) 66, 379, 390
NlaIII CATG 3 cut(s) 295, 673, 682
NlaIV GGNNCC 1 cut(s) 684
NmuCI GTSAC 1 cut(s) 52
NspI RCATGY 1 cut(s) 673
PagI TCATGA 1 cut(s) 291
PaqCI CACCTGC 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 2 cut(s) 323, 474
PfeI GAWTC 2 cut(s) 113, 153
PkrI GCNGC 2 cut(s) 301, 634
PleI GAGTC 2 cut(s) 345, 648
PpsI GAGTC 2 cut(s) 345, 648
Psp124BI GAGCTC 1 cut(s) 251
PspN4I GGNNCC 1 cut(s) 684
PspPI GGNCC 1 cut(s) 682
PsuI RGATCY 1 cut(s) 379
RsaI GTAC 3 cut(s) 49, 557, 601
RsaNI GTAC 3 cut(s) 48, 556, 600
SacI GAGCTC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 465
SatI GCNGC 2 cut(s) 300, 633
Sau3AI GATC 3 cut(s) 66, 379, 390
Sau96I GGNCC 1 cut(s) 682
ScaI AGTACT 1 cut(s) 601
SchI GAGTC 2 cut(s) 346, 649
SduI GDGCHC 1 cut(s) 251
SinI GGWCC 1 cut(s) 682
Sse9I AATT 2 cut(s) 34, 548
SsiI CCGC 1 cut(s) 300
SstI GAGCTC 1 cut(s) 251
StyI CCWWGG 2 cut(s) 198, 678
TaaI ACNGT 3 cut(s) 52, 439, 691
TaiI ACGT 1 cut(s) 148
TaqI TCGA 3 cut(s) 251, 365, 411
TasI AATT 2 cut(s) 34, 548
TatI WGTACW 1 cut(s) 599
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 2 cut(s) 113, 153
TscAI CASTG 1 cut(s) 642
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 632
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 204, 560
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 1 cut(s) 642
VpaK11BI GGWCC 1 cut(s) 682
XapI RAATTY 2 cut(s) 34, 548
XceI RCATGY 1 cut(s) 673
XcmI CCANNNNNNNNNTGG 1 cut(s) 490
XmnI GAANNNNTTC 2 cut(s) 323, 474
ZrmI AGTACT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.