Rroxscaffold_3G00227090

3-dehydroquinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001467
Physical Location & Seq
Forward (+)
11117848 .. 11125700
7853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227090.1

Sequence Viewer

Length: 876 bp
ATGGCGAAGCAATCTAAAGCCAAGAAATCGGAGAATTTAGGAAAGTGCCGAAGAGCCTCGCCGAGTTTGTCCGAGATTCGAAAAGTACAAAGCATTGGGGAGCAGAAGATGATTCGAGAAGCCAATGGCGGCCCCAACAAGGCTAACAAGCCGTTCATGCGCTCCTCATCGAGCAACCCCAATAAATTTATATTTAGCCACCTAGAGTTCATCAGTTGCTTCTTCTACGACGTCGTTTGGGAGCTCGAAGAAGATGGTGTGGGTTTGGACGGAGAGCAAGCAGGTCATGACGGCCGCCGTGGAGAGAGGCTGGAACACCTTCTGTTTCAAAGTCGAAACTCGCCGATGACTGGTCTTAAAGGGATTTTCGATAGCGAGAACACGAGAGTTGCCACAGTTGTTGAGGTTTCCAATCCCAAAGAGTTGGAAGAGCTTCAACCCAAAAACGGGGTGGCTGAGAATGTTTTTGTTGATTTGCTGGATTGGCAGGCAGGTGAGCTTATGAAATTTGAGTACCAAAAGAAGGTCGCCTCGCTGAGCAAGCTAAAGAAATGTAGTACTAACTCGAAGCATTGGAGAAATCAAGAGCAGCAGTCATGTGGGACTCATGTGATGTTAGTGGAAGTGTCGGCTTCCGCAGAGTCTAGTTCATCACAAGGCTCGACCGCCGAATGTCCGCGGGCTTTCAAGCTGGAAAACTTTGGGCTAGCGGGGTGTGATTGCTCTCACGTGGTAATTGGTGCTGGACCCGATGTTAGTTGGGTTCTTGCAAGCCACGAGTGTTCAAATGTTCTTGGACTGTTCCAATTGCAATATCTTGCCAATTTGCAGTCATGCACCAACTCCACTGAGCTGTACTTAACATCAGCGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHQS

Protein Analysis

291

Amino Acids

32.13

Weight (kDa)

6.41

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 482
AatII GACGTC 1 cut(s) 234
Acc36I ACCTGC 2 cut(s) 272, 482
AccII CGCG 1 cut(s) 679
AciI CCGC 8 cut(s) 129, 295, 636, 666, 677, 679, 710, 869
AcoI YGGCCR 1 cut(s) 292
AcsI RAATTY 3 cut(s) 34, 185, 506
AcvI CACGTG 1 cut(s) 730
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 4 cut(s) 87, 515, 559, 857
AfiI CCNNNNNNNGG 5 cut(s) 139, 350, 446, 447, 523
AgsI TTSAA 4 cut(s) 329, 437, 688, 786
AluBI AGCT 6 cut(s) 244, 433, 499, 544, 691, 853
AluI AGCT 6 cut(s) 244, 433, 499, 544, 691, 853
Alw21I GWGCWC 1 cut(s) 246
AoxI GGCC 2 cut(s) 130, 292
ApeKI GCWGC 1 cut(s) 589
ApoI RAATTY 3 cut(s) 34, 185, 506
Asp700I GAANNNNTTC 2 cut(s) 318, 432
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 2 cut(s) 131, 746
AsuHPI GGTGA 1 cut(s) 506
AsuII TTCGAA 1 cut(s) 79
AsuNHI GCTAGC 1 cut(s) 706
AvaII GGWCC 1 cut(s) 746
BanII GRGCYC 1 cut(s) 246
BauI CACGAG 2 cut(s) 382, 776
BbrPI CACGTG 1 cut(s) 730
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 601
BccI CCATC 1 cut(s) 248
BceAI ACGGC 3 cut(s) 136, 282, 307
BfaI CTAG 3 cut(s) 203, 645, 707
BfuAI ACCTGC 2 cut(s) 272, 482
BisI GCNGC 3 cut(s) 130, 295, 590
BlpI GCTNAGC 1 cut(s) 536
BlsI GCNGC 3 cut(s) 131, 296, 591
BmcAI AGTACT 1 cut(s) 559
Bme18I GGWCC 1 cut(s) 746
BmgT120I GGNCC 2 cut(s) 131, 746
BmiI GGNNCC 2 cut(s) 133, 748
BmtI GCTAGC 1 cut(s) 710
Bpu1102I GCTNAGC 1 cut(s) 536
Bpu14I TTCGAA 1 cut(s) 79
BsaAI YACGTR 1 cut(s) 730
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 298, 677
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 350, 446, 447, 523
Bse1I ACTGG 1 cut(s) 355
BseDI CCNNGG 2 cut(s) 298, 677
BseLI CCNNNNNNNGG 5 cut(s) 139, 350, 446, 447, 523
BseMII CTCAG 3 cut(s) 447, 527, 840
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 154
BseX3I CGGCCG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 601
Bsh1236I CGCG 1 cut(s) 679
Bsh1285I CGRYCG 2 cut(s) 295, 666
BshFI GGCC 2 cut(s) 132, 294
BsiEI CGRYCG 2 cut(s) 295, 666
BsiHKAI GWGCWC 1 cut(s) 246
BslFI GGGAC 1 cut(s) 616
BslI CCNNNNNNNGG 5 cut(s) 139, 350, 446, 447, 523
BsmFI GGGAC 1 cut(s) 616
BsnI GGCC 2 cut(s) 132, 294
Bsp119I TTCGAA 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 246
Bsp1720I GCTNAGC 1 cut(s) 536
BspACI CCGC 8 cut(s) 129, 295, 636, 666, 677, 679, 710, 869
BspANI GGCC 2 cut(s) 132, 294
BspCNI CTCAG 3 cut(s) 448, 528, 841
BspFNI CGCG 1 cut(s) 679
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 133, 748
BspMI ACCTGC 2 cut(s) 272, 482
BspOI GCTAGC 1 cut(s) 710
BspQI GCTCTTC 2 cut(s) 46, 423
BspT104I TTCGAA 1 cut(s) 79
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 298, 677
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 2 cut(s) 382, 776
Bst2BI CACGAG 2 cut(s) 382, 776
Bst4CI ACNGT 2 cut(s) 397, 801
Bst6I CTCTTC 2 cut(s) 46, 423
BstACI GRCGYC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 730
BstBI TTCGAA 1 cut(s) 79
BstC8I GCNNGC 6 cut(s) 279, 489, 542, 681, 708, 772
BstDEI CTNAG 3 cut(s) 456, 536, 849
BstDSI CCRYGG 2 cut(s) 298, 677
BstFNI CGCG 1 cut(s) 679
BstHHI GCGC 1 cut(s) 162
BstMCI CGRYCG 2 cut(s) 295, 666
BstMWI GCNNNNNNNGC 3 cut(s) 157, 484, 541
BstUI CGCG 1 cut(s) 679
BstV1I GCAGC 1 cut(s) 601
BstXI CCANNNNNNTGG 1 cut(s) 424
BstZI CGGCCG 1 cut(s) 292
BsuRI GGCC 2 cut(s) 132, 294
BtgI CCRYGG 2 cut(s) 298, 677
BtsIMutI CAGTG 1 cut(s) 846
BveI ACCTGC 2 cut(s) 272, 482
Cac8I GCNNGC 6 cut(s) 279, 489, 542, 681, 708, 772
CciI TCATGA 1 cut(s) 286
CfoI GCGC 1 cut(s) 162
Cfr13I GGNCC 2 cut(s) 131, 746
Cfr42I CCGCGG 1 cut(s) 680
Csp6I GTAC 4 cut(s) 86, 514, 558, 856
CspCI CAANNNNNGTGG 2 cut(s) 382, 417
CviAII CATG 5 cut(s) 157, 287, 597, 608, 834
CviQI GTAC 4 cut(s) 86, 514, 558, 856
DdeI CTNAG 3 cut(s) 456, 536, 849
EaeI YGGCCR 1 cut(s) 292
EagI CGGCCG 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 46, 423
EarI CTCTTC 2 cut(s) 46, 423
Ecl136II GAGCTC 1 cut(s) 244
EclXI CGGCCG 1 cut(s) 292
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 746
Eco52I CGGCCG 1 cut(s) 292
Eco53kI GAGCTC 1 cut(s) 244
Eco72I CACGTG 1 cut(s) 730
EcoICRI GAGCTC 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 246
FaeI CATG 5 cut(s) 160, 290, 600, 611, 837
FaiI YATR 7 cut(s) 158, 191, 288, 503, 598, 609, 835
FaqI GGGAC 1 cut(s) 616
FatI CATG 5 cut(s) 156, 286, 596, 607, 833
FauI CCCGC 2 cut(s) 672, 703
Fnu4HI GCNGC 3 cut(s) 130, 295, 590
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 3 cut(s) 130, 295, 590
FspBI CTAG 3 cut(s) 203, 645, 707
GlaI GCGC 1 cut(s) 161
GluI GCNGC 3 cut(s) 130, 295, 590
HaeIII GGCC 2 cut(s) 132, 294
HhaI GCGC 1 cut(s) 162
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 5 cut(s) 160, 290, 600, 611, 837
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 4 cut(s) 76, 112, 604, 641
HphI GGTGA 1 cut(s) 506
Hpy188I TCNGA 2 cut(s) 31, 73
Hpy188III TCNNGA 3 cut(s) 116, 287, 584
Hpy99I CGWCG 2 cut(s) 233, 236
HpyAV CCTTC 2 cut(s) 329, 517
HpyCH4III ACNGT 2 cut(s) 397, 801
HpyCH4IV ACGT 2 cut(s) 231, 729
HpyCH4V TGCA 4 cut(s) 770, 811, 829, 837
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 484, 541
HpyF3I CTNAG 3 cut(s) 456, 536, 849
HpySE526I ACGT 2 cut(s) 231, 729
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 5 cut(s) 160, 290, 600, 611, 837
HspAI GCGC 1 cut(s) 160
KspI CCGCGG 1 cut(s) 680
LguI GCTCTTC 2 cut(s) 46, 423
LmnI GCTCC 3 cut(s) 100, 167, 241
LpnPI CCDG 8 cut(s) 267, 296, 336, 464, 473, 477, 677, 729
Lsp1109I GCAGC 1 cut(s) 601
MaeI CTAG 3 cut(s) 203, 645, 707
MaeII ACGT 2 cut(s) 231, 729
MboII GAAGA 6 cut(s) 63, 118, 214, 260, 263, 440
MfeI CAATTG 1 cut(s) 806
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 6 cut(s) 34, 185, 506, 735, 806, 823
MlyI GAGTC 2 cut(s) 598, 650
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 5 cut(s) 67, 175, 300, 397, 541
MroXI GAANNNNTTC 2 cut(s) 318, 432
MseI TTAA 2 cut(s) 357, 860
MspA1I CMGCKG 2 cut(s) 679, 869
MunI CAATTG 1 cut(s) 806
MvnI CGCG 1 cut(s) 679
MwoI GCNNNNNNNGC 3 cut(s) 157, 484, 541
NheI GCTAGC 1 cut(s) 706
NlaIII CATG 5 cut(s) 160, 290, 600, 611, 837
NlaIV GGNNCC 2 cut(s) 133, 748
NmeAIII GCCGAG 1 cut(s) 87
NspV TTCGAA 1 cut(s) 79
PagI TCATGA 1 cut(s) 286
PaqCI CACCTGC 1 cut(s) 482
PciSI GCTCTTC 2 cut(s) 46, 423
PdmI GAANNNNTTC 2 cut(s) 318, 432
PfeI GAWTC 2 cut(s) 76, 112
PkrI GCNGC 3 cut(s) 131, 296, 591
PleI GAGTC 2 cut(s) 598, 649
PmaCI CACGTG 1 cut(s) 730
PmlI CACGTG 1 cut(s) 730
PpsI GAGTC 2 cut(s) 598, 649
Ppu21I YACGTR 1 cut(s) 730
Psp124BI GAGCTC 1 cut(s) 246
PspCI CACGTG 1 cut(s) 730
PspN4I GGNNCC 2 cut(s) 133, 748
PspPI GGNCC 2 cut(s) 131, 746
RsaI GTAC 4 cut(s) 87, 515, 559, 857
RsaNI GTAC 4 cut(s) 86, 514, 558, 856
SacI GAGCTC 1 cut(s) 246
SacII CCGCGG 1 cut(s) 680
SapI GCTCTTC 2 cut(s) 46, 423
SaqAI TTAA 2 cut(s) 357, 860
SatI GCNGC 3 cut(s) 130, 295, 590
Sau96I GGNCC 2 cut(s) 131, 746
ScaI AGTACT 1 cut(s) 559
SchI GAGTC 2 cut(s) 598, 650
SduI GDGCHC 1 cut(s) 246
Sfr303I CCGCGG 1 cut(s) 680
SfuI TTCGAA 1 cut(s) 79
SgrBI CCGCGG 1 cut(s) 680
SinI GGWCC 1 cut(s) 746
Sse9I AATT 6 cut(s) 34, 185, 506, 735, 806, 823
SsiI CCGC 8 cut(s) 129, 295, 636, 666, 677, 679, 710, 869
SspMI CTAG 3 cut(s) 203, 645, 707
SstI GAGCTC 1 cut(s) 246
TaaI ACNGT 2 cut(s) 397, 801
TaiI ACGT 2 cut(s) 234, 732
TaqI TCGA 8 cut(s) 79, 115, 170, 246, 334, 369, 566, 662
TasI AATT 6 cut(s) 34, 185, 506, 735, 806, 823
TatI WGTACW 3 cut(s) 85, 557, 855
TauI GCSGC 2 cut(s) 132, 297
TfiI GAWTC 2 cut(s) 76, 112
Tru1I TTAA 2 cut(s) 357, 860
Tru9I TTAA 2 cut(s) 357, 860
TscAI CASTG 1 cut(s) 853
TseI GCWGC 1 cut(s) 589
TspDTI ATGAA 4 cut(s) 145, 199, 518, 639
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 1 cut(s) 853
VpaK11BI GGWCC 1 cut(s) 746
XapI RAATTY 3 cut(s) 34, 185, 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XmnI GAANNNNTTC 2 cut(s) 318, 432
XspI CTAG 3 cut(s) 203, 645, 707
ZraI GACGTC 1 cut(s) 232
ZrmI AGTACT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.