Rh4DG196700

3-dehydroquinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001467
Physical Location & Seq
Forward (+)
38373774 .. 38374431
658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG196700.1

Sequence Viewer

Length: 354 bp
ATGGAAACCAAGGCCACCCAGAGTTCATCAGTTGCTTCTTCTACGACGTCGTTTGGGAGCTCGAAGAAGACGGTGTGGGTTTGGACGGAGAGCAAGCAGGTCATGACGGCCGCCGTGGAGAGAGGCTGGAACACCTTCCTGTTTCAGAGTCAGAAACTCGCCAATGACTGGTCTTCGATTGCATTGATAGATCCTCTTTTGATCAAAGAGGGAGGGATTTTTGATAGCGAGAACACGAGAGTTGCCATAGTTGTTGAGGTTTCCAACCCCAAAGAGTTGGAAGAGCTTCAACCCGAAAACGGGGTGGTTGAGAATGTTGTTGTTGATTTGCTGGATTGGCAGAGTGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHQS

Protein Analysis

117

Amino Acids

12.95

Weight (kDa)

4.4

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHQS PF01959 22 - 114 6.4e-19 3-dehydroquinate synthase II N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 88
AccB7I CCANNNNNTGG 1 cut(s) 168
AciI CCGC 1 cut(s) 111
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 108
AcyI GRCGYC 1 cut(s) 47
AfiI CCNNNNNNNGG 3 cut(s) 168, 299, 300
AgsI TTSAA 1 cut(s) 290
AluBI AGCT 2 cut(s) 60, 286
AluI AGCT 2 cut(s) 60, 286
Alw21I GWGCWC 1 cut(s) 62
AlwI GGATC 1 cut(s) 185
AoxI GGCC 2 cut(s) 12, 108
Asp700I GAANNNNTTC 2 cut(s) 134, 285
BanII GRGCYC 1 cut(s) 62
BauI CACGAG 1 cut(s) 235
BbsI GAAGAC 2 cut(s) 74, 165
Bbv12I GWGCWC 1 cut(s) 62
BceAI ACGGC 2 cut(s) 98, 123
BclI TGATCA 1 cut(s) 201
BfuAI ACCTGC 1 cut(s) 88
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
BpiI GAAGAC 2 cut(s) 74, 165
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 9, 114
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 299, 300
Bse1I ACTGG 1 cut(s) 173
BseDI CCNNGG 2 cut(s) 9, 114
BseLI CCNNNNNNNGG 3 cut(s) 168, 299, 300
BseNI ACTGG 1 cut(s) 173
BseX3I CGGCCG 1 cut(s) 108
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 14, 110
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 1 cut(s) 62
BslI CCNNNNNNNGG 3 cut(s) 168, 299, 300
BsnI GGCC 2 cut(s) 14, 110
Bsp1286I GDGCHC 1 cut(s) 62
Bsp143I GATC 2 cut(s) 190, 201
BspACI CCGC 1 cut(s) 111
BspANI GGCC 2 cut(s) 14, 110
BspHI TCATGA 1 cut(s) 102
BspMI ACCTGC 1 cut(s) 88
BspPI GGATC 1 cut(s) 185
BspQI GCTCTTC 1 cut(s) 276
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 9, 114
BssMI GATC 2 cut(s) 190, 201
BssNI GRCGYC 1 cut(s) 47
BssSI CACGAG 1 cut(s) 235
BssT1I CCWWGG 1 cut(s) 9
Bst2BI CACGAG 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 95
BstDSI CCRYGG 1 cut(s) 114
BstKTI GATC 2 cut(s) 193, 204
BstMBI GATC 2 cut(s) 190, 201
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 1 cut(s) 337
BstV2I GAAGAC 2 cut(s) 74, 165
BstX2I RGATCY 1 cut(s) 190
BstXI CCANNNNNNTGG 1 cut(s) 277
BstYI RGATCY 1 cut(s) 190
BstZI CGGCCG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 14, 110
BtgI CCRYGG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 88
Cac8I GCNNGC 1 cut(s) 95
CciI TCATGA 1 cut(s) 102
CviAII CATG 1 cut(s) 103
CviJI RGCY 5 cut(s) 14, 60, 110, 126, 286
CviKI_1 RGCY 5 cut(s) 14, 60, 110, 126, 286
DpnI GATC 2 cut(s) 192, 203
DpnII GATC 2 cut(s) 190, 201
EaeI YGGCCR 1 cut(s) 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 60
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 62
Eco52I CGGCCG 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 62
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 1 cut(s) 106
FaiI YATR 2 cut(s) 104, 248
FatI CATG 1 cut(s) 102
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 1 cut(s) 111
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 1 cut(s) 111
GluI GCNGC 1 cut(s) 111
HaeIII GGCC 2 cut(s) 14, 110
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 1 cut(s) 106
HinfI GANTC 1 cut(s) 148
Hpy188I TCNGA 2 cut(s) 147, 153
Hpy188III TCNNGA 1 cut(s) 103
Hpy99I CGWCG 2 cut(s) 49, 52
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 1 cut(s) 337
HpySE526I ACGT 1 cut(s) 47
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 2 cut(s) 190, 201
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 1 cut(s) 57
LpnPI CCDG 6 cut(s) 32, 83, 112, 152, 154, 317
MaeII ACGT 1 cut(s) 47
MalI GATC 2 cut(s) 192, 203
MboI GATC 2 cut(s) 190, 201
MboII GAAGA 5 cut(s) 30, 76, 79, 165, 293
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 62
MlyI GAGTC 1 cut(s) 157
MmeI TCCRAC 2 cut(s) 258, 288
MnlI CCTC 5 cut(s) 116, 202, 204, 206, 250
MroXI GAANNNNTTC 2 cut(s) 134, 285
MwoI GCNNNNNNNGC 1 cut(s) 337
NdeII GATC 2 cut(s) 190, 201
NlaIII CATG 1 cut(s) 106
PagI TCATGA 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 276
PdmI GAANNNNTTC 2 cut(s) 134, 285
PflMI CCANNNNNTGG 1 cut(s) 168
PkrI GCNGC 1 cut(s) 112
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 62
PsuI RGATCY 1 cut(s) 190
SacI GAGCTC 1 cut(s) 62
SapI GCTCTTC 1 cut(s) 276
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 2 cut(s) 190, 201
SchI GAGTC 1 cut(s) 157
SduI GDGCHC 1 cut(s) 62
SetI ASST 6 cut(s) 50, 62, 102, 137, 261, 288
SsiI CCGC 1 cut(s) 111
SstI GAGCTC 1 cut(s) 62
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 1 cut(s) 50
TaqI TCGA 2 cut(s) 62, 176
TauI GCSGC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 15
TspGWI ACGGA 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 168
XmnI GAANNNNTTC 2 cut(s) 134, 285
ZraI GACGTC 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.