Rroxscaffold_4G00301620

3-dehydroquinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001467
Physical Location & Seq
Reverse (-)
21952959 .. 21964349
11391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301620.1

Sequence Viewer

Length: 741 bp
ATGGCGAAGCAATCTAAAGCCAAGAAATCGGAGAATTTGGGAAAGTGTGCCGAAGAGCCTGCTAAGTTTGTCCGAGATTCGAAAAGTACAAAGCATTGGGGAGCAGAGAAGATGATTCGAGAAGGCAATGGCGGCCCCAACAAGGCTAACAAGCCGTTCATGCGCTCCTCATCGAGCAACCCCAATAAATGCCACCCGAGTTCATCGGTTGCTTCTTCTACGACGTCATTTGGGAGCTCGAAGAAGATGGTGTGGGTTTGGACGGAGAGCAAGCAGGTCATGACGGCCGCCGTGGAGAGAGGCTGGAACACCTTCCCGTTTCAAAGTCGAAACTCGCCGATGACTGGTCTTAAGTCTTCTCGACCTTTTTTGTTCTATTACGAACTATACCGATCATGTGGGCAAGAAATATATTTTCCTACTCGAGCTAGATATGGTATTATTCCCTCAAGGGAGGAACCCAACTTTGATGAGATAGCAGCTACACCCAATACTTCAGTAACTGCAAAGAACTCGGGCATGCCAATAATGCTTGTCCGGTTCCACACAGGCCCCCAGGTATTGAGAGTCCACAGGGGTAACCTTCTGCCCAGCCACACAGGTCAACCCTCCGACATCCAAACGTGCTTGTTCCCATTCGTTCCTTCTGACGGCCAAGCGTTATCCAAACACCACGCATTCATGGGAGTAGACTTCTGGCCAAAAGGTGGCCAATACACTGCAGACGATGTGGCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHQS

Protein Analysis

246

Amino Acids

27.35

Weight (kDa)

9.76

Isoelectric Point (pI)

56.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 227
Acc36I ACCTGC 1 cut(s) 265
AccB7I CCANNNNNTGG 1 cut(s) 707
AccI GTMKAC 1 cut(s) 690
AciI CCGC 2 cut(s) 132, 288
AcoI YGGCCR 5 cut(s) 285, 652, 698, 709, 732
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 480
AcyI GRCGYC 1 cut(s) 224
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 4 cut(s) 142, 344, 650, 707
AflII CTTAAG 1 cut(s) 350
AgsI TTSAA 1 cut(s) 323
AjnI CCWGG 1 cut(s) 555
AluBI AGCT 3 cut(s) 237, 428, 482
AluI AGCT 3 cut(s) 237, 428, 482
Alw21I GWGCWC 1 cut(s) 239
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 3 cut(s) 196, 423, 514
AoxI GGCC 7 cut(s) 133, 285, 550, 652, 698, 709, 732
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 34
Asp700I GAANNNNTTC 1 cut(s) 311
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 134, 551
AsuII TTCGAA 1 cut(s) 80
AvaI CYCGRG 3 cut(s) 196, 423, 514
BalI TGGCCA 3 cut(s) 700, 711, 734
BanII GRGCYC 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 239
BbvI GCAGC 1 cut(s) 491
BccI CCATC 1 cut(s) 241
BceAI ACGGC 4 cut(s) 139, 275, 300, 667
BcgI CGANNNNNNTGC 4 cut(s) 41, 75, 495, 529
BciT130I CCWGG 1 cut(s) 557
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 720
BfrI CTTAAG 1 cut(s) 350
BfuAI ACCTGC 1 cut(s) 265
BisI GCNGC 3 cut(s) 133, 288, 480
BlsI GCNGC 3 cut(s) 134, 289, 481
Bme1390I CCNGG 1 cut(s) 557
BmeT110I CYCGRG 3 cut(s) 196, 423, 514
BmgT120I GGNCC 2 cut(s) 134, 551
BmiI GGNNCC 4 cut(s) 136, 459, 542, 553
BmrFI CCNGG 1 cut(s) 557
BpiI GAAGAC 1 cut(s) 348
Bpu14I TTCGAA 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 433
BsaHI GRCGYC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 291, 555
BsaWI WCCGGW 1 cut(s) 537
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 344, 650, 707
Bse1I ACTGG 1 cut(s) 349
Bse3DI GCAATG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 2 cut(s) 291, 555
BseGI GGATG 1 cut(s) 615
BseLI CCNNNNNNNGG 4 cut(s) 142, 344, 650, 707
BseMI GCAATG 1 cut(s) 133
BseNI ACTGG 1 cut(s) 349
BseRI GAGGAG 1 cut(s) 157
BseX3I CGGCCG 1 cut(s) 285
BseXI GCAGC 1 cut(s) 491
BseYI CCCAGC 1 cut(s) 590
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 7 cut(s) 135, 287, 552, 654, 700, 711, 734
BsiEI CGRYCG 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 239
BsiHKCI CYCGRG 3 cut(s) 196, 423, 514
BsiSI CCGG 1 cut(s) 538
BslI CCNNNNNNNGG 4 cut(s) 142, 344, 650, 707
BsmI GAATGC 1 cut(s) 677
BsnI GGCC 7 cut(s) 135, 287, 552, 654, 700, 711, 734
BsoBI CYCGRG 3 cut(s) 196, 423, 514
Bsp119I TTCGAA 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 239
Bsp143I GATC 1 cut(s) 392
BspACI CCGC 2 cut(s) 132, 288
BspANI GGCC 7 cut(s) 135, 287, 552, 654, 700, 711, 734
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 4 cut(s) 136, 459, 542, 553
BspMAI CTGCAG 1 cut(s) 724
BspMI ACCTGC 1 cut(s) 265
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 80
BspTI CTTAAG 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 133
BsrI ACTGG 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 291, 555
BssMI GATC 1 cut(s) 392
BssNI GRCGYC 1 cut(s) 224
Bst2UI CCWGG 1 cut(s) 557
Bst6I CTCTTC 1 cut(s) 48
BstACI GRCGYC 1 cut(s) 224
BstAFI CTTAAG 1 cut(s) 350
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 3 cut(s) 60, 272, 521
BstDEI CTNAG 1 cut(s) 63
BstDSI CCRYGG 1 cut(s) 291
BstEII GGTNACC 1 cut(s) 578
BstF5I GGATG 1 cut(s) 615
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 1 cut(s) 395
BstMBI GATC 1 cut(s) 392
BstMCI CGRYCG 1 cut(s) 288
BstMWI GCNNNNNNNGC 3 cut(s) 132, 160, 529
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 523
BstPI GGTNACC 1 cut(s) 578
BstSCI CCNGG 1 cut(s) 555
BstSFI CTRYAG 1 cut(s) 720
BstV1I GCAGC 1 cut(s) 491
BstV2I GAAGAC 1 cut(s) 348
BstZI CGGCCG 1 cut(s) 285
BsuRI GGCC 7 cut(s) 135, 287, 552, 654, 700, 711, 734
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 1 cut(s) 615
BtsI GCAGTG 1 cut(s) 717
BtsIMutI CAGTG 1 cut(s) 717
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 3 cut(s) 60, 272, 521
CaiI CAGNNNCTG 1 cut(s) 503
CciI TCATGA 1 cut(s) 279
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 134, 551
Csp6I GTAC 1 cut(s) 87
CviAII CATG 5 cut(s) 160, 280, 396, 520, 682
CviQI GTAC 1 cut(s) 87
DdeI CTNAG 1 cut(s) 63
DpnI GATC 1 cut(s) 394
DpnII GATC 1 cut(s) 392
EaeI YGGCCR 5 cut(s) 285, 652, 698, 709, 732
EagI CGGCCG 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 237
EclXI CGGCCG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 239
Eco52I CGGCCG 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 480
Eco88I CYCGRG 3 cut(s) 196, 423, 514
Eco91I GGTNACC 1 cut(s) 578
EcoICRI GAGCTC 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 551
EcoO65I GGTNACC 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 555
EcoT38I GRGCYC 1 cut(s) 239
FaeI CATG 5 cut(s) 163, 283, 399, 523, 685
FaiI YATR 8 cut(s) 161, 281, 388, 397, 412, 435, 521, 683
FatI CATG 5 cut(s) 159, 279, 395, 519, 681
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 3 cut(s) 133, 288, 480
FokI GGATG 1 cut(s) 602
FriOI GRGCYC 1 cut(s) 239
Fsp4HI GCNGC 3 cut(s) 133, 288, 480
FspBI CTAG 1 cut(s) 429
GlaI GCGC 1 cut(s) 164
GluI GCNGC 3 cut(s) 133, 288, 480
GsaI CCCAGC 1 cut(s) 594
HaeIII GGCC 7 cut(s) 135, 287, 552, 654, 700, 711, 734
HapII CCGG 1 cut(s) 538
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 224
Hin1II CATG 5 cut(s) 163, 283, 399, 523, 685
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 1 cut(s) 605
HindII GTYRAC 1 cut(s) 605
HinfI GANTC 3 cut(s) 77, 115, 567
HpaII CCGG 1 cut(s) 538
Hpy166II GTNNAC 3 cut(s) 571, 605, 691
Hpy188I TCNGA 4 cut(s) 31, 74, 613, 649
Hpy188III TCNNGA 3 cut(s) 119, 280, 360
Hpy8I GTNNAC 3 cut(s) 571, 605, 691
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 4 cut(s) 116, 322, 593, 654
HpyCH4IV ACGT 2 cut(s) 224, 623
HpyCH4V TGCA 2 cut(s) 506, 722
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 160, 529
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 2 cut(s) 224, 623
Hsp92I GRCGYC 1 cut(s) 224
Hsp92II CATG 5 cut(s) 163, 283, 399, 523, 685
HspAI GCGC 1 cut(s) 163
Kzo9I GATC 1 cut(s) 392
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 3 cut(s) 101, 170, 234
Lsp1109I GCAGC 1 cut(s) 491
MaeI CTAG 1 cut(s) 429
MaeII ACGT 2 cut(s) 224, 623
MaeIII GTNAC 2 cut(s) 499, 578
MalI GATC 1 cut(s) 394
MboI GATC 1 cut(s) 392
MboII GAAGA 6 cut(s) 65, 121, 207, 253, 256, 348
MhlI GDGCHC 1 cut(s) 239
MlsI TGGCCA 3 cut(s) 700, 711, 734
MluCI AATT 1 cut(s) 34
MluNI TGGCCA 3 cut(s) 700, 711, 734
MlyI GAGTC 1 cut(s) 576
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 5 cut(s) 178, 293, 448, 457, 619
Mox20I TGGCCA 3 cut(s) 700, 711, 734
MroXI GAANNNNTTC 1 cut(s) 311
MscI TGGCCA 3 cut(s) 700, 711, 734
MseI TTAA 1 cut(s) 351
Msp20I TGGCCA 3 cut(s) 700, 711, 734
MspCI CTTAAG 1 cut(s) 350
MspI CCGG 1 cut(s) 538
MspR9I CCNGG 1 cut(s) 557
Mva1269I GAATGC 1 cut(s) 677
MvaI CCWGG 1 cut(s) 557
MwoI GCNNNNNNNGC 3 cut(s) 132, 160, 529
NdeII GATC 1 cut(s) 392
NlaIII CATG 5 cut(s) 163, 283, 399, 523, 685
NlaIV GGNNCC 4 cut(s) 136, 459, 542, 553
NspI RCATGY 1 cut(s) 523
NspV TTCGAA 1 cut(s) 80
PaeI GCATGC 1 cut(s) 523
PaeR7I CTCGAG 1 cut(s) 423
PagI TCATGA 1 cut(s) 279
PciSI GCTCTTC 1 cut(s) 48
PctI GAATGC 1 cut(s) 677
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 2 cut(s) 77, 115
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 3 cut(s) 134, 289, 481
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 555
PspEI GGTNACC 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 590
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 4 cut(s) 136, 459, 542, 553
PspPI GGNCC 2 cut(s) 134, 551
PspXI VCTCGAGB 1 cut(s) 423
PstI CTGCAG 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 503
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
SacI GAGCTC 1 cut(s) 239
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 3 cut(s) 133, 288, 480
Sau3AI GATC 1 cut(s) 392
Sau96I GGNCC 2 cut(s) 134, 551
SchI GAGTC 1 cut(s) 576
ScrFI CCNGG 1 cut(s) 557
SduI GDGCHC 1 cut(s) 239
SfcI CTRYAG 1 cut(s) 720
Sfr274I CTCGAG 1 cut(s) 423
SfuI TTCGAA 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 423
SmlI CTYRAG 3 cut(s) 350, 423, 448
SmoI CTYRAG 3 cut(s) 350, 423, 448
SphI GCATGC 1 cut(s) 523
Sse9I AATT 1 cut(s) 34
SsiI CCGC 2 cut(s) 132, 288
SspMI CTAG 1 cut(s) 429
SstI GAGCTC 1 cut(s) 239
StyD4I CCNGG 1 cut(s) 555
TaiI ACGT 2 cut(s) 227, 626
TaqI TCGA 7 cut(s) 80, 118, 173, 239, 328, 361, 424
TasI AATT 1 cut(s) 34
TatI WGTACW 1 cut(s) 86
TauI GCSGC 2 cut(s) 135, 290
TfiI GAWTC 2 cut(s) 77, 115
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 724
TseI GCWGC 1 cut(s) 479
TspDTI ATGAA 3 cut(s) 148, 192, 670
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 1 cut(s) 724
Van91I CCANNNNNTGG 1 cut(s) 707
Vha464I CTTAAG 1 cut(s) 350
XapI RAATTY 1 cut(s) 34
XceI RCATGY 1 cut(s) 523
XhoI CTCGAG 1 cut(s) 423
XmiI GTMKAC 1 cut(s) 690
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 1 cut(s) 429
ZraI GACGTC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.