Rroxscaffold_6G00403640

Pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000378
Physical Location & Seq
Forward (+)
26117777 .. 26120093
2317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403640.1

Sequence Viewer

Length: 1200 bp
ATGATGAAGCACTCTCATTTCAGCTCCCTATTCAACACTCTCTACTCCCGTTTCTGTCACGTGTCGCTGTACTCAACGGCGGCACCCAAATCTCATCGGAAGCTCTGGAAGGACTCGCTGAACACCCTCTACCGTCGGATCACGGGGAGCGAATTTAAGGCCTCCGTTTTACCGATTCTCGACCGGTGGGTCCAAGAAGGCCAAACTGTAGATAGAGATGACCTCGTCACTATCATCAAGGAGCTCAGGTACTACAAACACTACAGACGAGCTCTTGAGGTGTCTATGTGGATGAGTGACAAAAGGTATGTTGAACTTTTGCCCTCTGACGTAGCCATCCGGCTCGACATGATTGCAAAAGTTCATGGAATAGAACATGCTGAGAAGTATTTTAACAACACTCCCGAACAATTGAAAGCCTATCAGGTTTACTATGCTCTCCTCAACTGCTATGCTCATGCAAAAAAGTTGGAAAAAGCAGACGCCACCATGCAGAAGATGAGGGATTTGGGTTTCTGCAGGACGTCATGGTCATACAATGCTTTGCTCAATTTGTATTATAAGACCGGAAACCATGAGAAATTTGACAATCTGATGAGTGAAATGGAAGAGAGTGGCATCAGGTTTGACAGATTCACATATGGGATCAGACTCAGTGCAGCTGCAGCTGCTTCTGATCTCAAGGGAATTGACAAGATTCTGGCAGAATGGGAATCCGATCCTAAGAGTCTTGTGGACTGGACTGGTTATGCTGTTGCAGCAAATGGTTATACAAAAGCAGGAGCTGTGGACAAGGCTTTAGCAATGCTAAGGAGATCTGAGGAACAGATACCAAGTTCTAAAAGACAGAGGCAAGCATATGAGCGCCTTATGACGCAATATGCAGTACTAGGGAAGAAAGACGATACTTTGAGACTATGGAAATGTTACAAGGAGCAGATGAAAGTATACAATAAGGGTTATCTATGCGTAATGACCTCACTCTTGAAGACTGATGACATTGAGAGTGCTGAAAAGATCTATGAAGAGTGGGAATCTGAGCAAGCAATATATGATATTCGTATACCCAATACCTTGATTGGTGCTTATACCAGGAAAGGTTTTCTTGACAAGGCTGAAGCCATTATGAGCAGGATAAGATTGAAGGATGGGAACCCTAATCCTACAAGATGGATTTATTTGGCGAAAATGGTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

399

Amino Acids

46.6

Weight (kDa)

9.16

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 178 - 219 3e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000378)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20710 AT2G20710 AT2G20720
fragaria_vesca FvH4_6g21294 FvH4_6g21300 FvH4_6g21300 FvH4_6g21300
malus_domestica MD02G1294300.v1.1 MD05G1022100.v1.1 MD05G1022200.v1.1 MD05G1046500.v1.1 MD09G1248800.v1.1 MD12G1049300.v1.1 MD17G1241400.v1.1
prunus_persica Prupe.3G136900_v2.0.a1 Prupe.3G136900_v2.0.a1 Prupe.3G136900_v2.0.a1 Prupe.3G136900_v2.0.a1 Prupe.3G136900_v2.0.a1 Prupe.7G086900_v2.0.a1 Prupe.8G026200_v2.0.a1 Prupe.8G026200_v2.0.a1 Prupe.8G026200_v2.0.a1 Prupe.8G026200_v2.0.a1 Prupe.8G026200_v2.0.a1
pyrus_communis pycom02g24780 pycom05g01290 pycom05g01300 pycom09g16570 pycom12g04420 pycom14g03980 pycom17g24460
rosa_chinensis RchiOBHm_Chr3g0477821 RchiOBHm_Chr3g0477851 RchiOBHm_Chr3g0477861 RchiOBHm_Chr3g0477891 RchiOBHm_Chr6g0275941 RchiOBHm_Chr6g0276181
rosa_laevigata RLG00000013421 RLG00000013422 RLG00000023681 RLG00000023684 RLG00000023685 RLG00000023686 RLG00000023687 RLG00000023689
rosa_multiflora Rmu_co8131942.1_g000001 Rmu_co8189010.1_g000001 Rmu_co8432533.1_g000001 Rmu_sc0002030.1_g000001 Rmu_sc0002030.1_g000002 Rmu_sc0005991.1_g000015 Rmu_sc0005991.1_g000019 Rmu_sc0005991.1_g000020 Rmu_sc0005991.1_g000021 Rmu_sc0017665.1_g000003 Rmu_sc0017665.1_g000006 Rmu_sc0017665.1_g000007 Rmu_sc0017867.1_g000002 Rmu_sc0017867.1_g000003 Rmu_sc0022989.1_g000003 Rmu_sc0022990.1_g000001 Rmu_ssc0000201.1_g000012 Rmu_ssc0000201.1_g000013 Rmu_ssc0000201.1_g000014
rosa_roxburghii Rroxscaffold_6G00403620 Rroxscaffold_6G00403640 Rroxscaffold_6G00403650 Rroxscaffold_6G00403660 Rroxscaffold_6G00403690 Rroxscaffold_7G00192760
rosa_rugosa Rorug03G0163500 Rorug03G0163600 Rorug03G0163800 Rorug06G0097700
rosa_samantha Rh3AG213800 Rh3AG214000 Rh3AG214100 Rh3AG214200 Rh3AG214400 Rh3BG247600 Rh3BG247700 Rh3BG248000 Rh3CG241100 Rh3CG241500 Rh3CG241700 Rh3CG242000 Rh3DG240500 Rh3DG240900 Rh3DG241000 Rh3DG241100 Rh3DG241300 Rh6AG209100 Rh6AG210500 Rh6BG213200 Rh6BG214700 Rh6CG216100 Rh6CG217400 Rh6CG217500 Rh6DG205500 Rh6DG207000
rosa_wichuraiana Rw3G019320 Rw6G018230 Rw6G018340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 561
AasI GACNNNNNNGTC 2 cut(s) 188, 529
AatII GACGTC 1 cut(s) 527
AccB1I GGYRCC 1 cut(s) 82
AccI GTMKAC 2 cut(s) 948, 1063
AciI CCGC 1 cut(s) 80
AclWI GGATC 3 cut(s) 146, 653, 713
AcsI RAATTY 2 cut(s) 152, 581
AcuI CTGAAG 1 cut(s) 1137
AcvI CACGTG 1 cut(s) 61
AcyI GRCGYC 2 cut(s) 483, 524
AfaI GTAC 3 cut(s) 71, 251, 888
AflIII ACRYGT 1 cut(s) 60
AgeI ACCGGT 1 cut(s) 183
AgsI TTSAA 5 cut(s) 34, 314, 415, 988, 1144
AjnI CCWGG 1 cut(s) 1091
AluBI AGCT 7 cut(s) 24, 103, 244, 272, 662, 668, 785
AluI AGCT 7 cut(s) 24, 103, 244, 272, 662, 668, 785
Alw21I GWGCWC 2 cut(s) 246, 274
Alw26I GTCTC 1 cut(s) 907
AlwI GGATC 3 cut(s) 146, 653, 713
AlwNI CAGNNNCTG 1 cut(s) 785
AoxI GGCC 2 cut(s) 159, 199
ApeKI GCWGC 5 cut(s) 659, 662, 665, 668, 758
ApoI RAATTY 2 cut(s) 152, 581
AsiGI ACCGGT 1 cut(s) 183
AspLEI GCGC 1 cut(s) 867
AspS9I GGNCC 1 cut(s) 190
AvaII GGWCC 1 cut(s) 190
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 2 cut(s) 246, 274
BbrPI CACGTG 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 995
Bbv12I GWGCWC 2 cut(s) 246, 274
BbvI GCAGC 5 cut(s) 649, 655, 671, 677, 770
BccI CCATC 3 cut(s) 344, 1142, 1164
BceAI ACGGC 1 cut(s) 93
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BciT130I CCWGG 1 cut(s) 1093
BcoDI GTCTC 1 cut(s) 907
BfaI CTAG 1 cut(s) 890
BfmI CTRYAG 4 cut(s) 207, 262, 517, 663
BfoI RGCGCY 1 cut(s) 868
BglII AGATCT 2 cut(s) 815, 1017
BisI GCNGC 6 cut(s) 81, 660, 663, 666, 669, 759
BlsI GCNGC 6 cut(s) 82, 661, 664, 667, 670, 760
BmcAI AGTACT 1 cut(s) 888
Bme1390I CCNGG 1 cut(s) 1093
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 3 cut(s) 84, 191, 1154
BmrFI CCNGG 1 cut(s) 1093
BmsI GCATC 1 cut(s) 627
BpiI GAAGAC 1 cut(s) 995
BplI GAGNNNNNCTC 2 cut(s) 207, 239
Bpu10I CCTNAGC 2 cut(s) 245, 809
BpuEI CTTGAG 2 cut(s) 296, 665
BsaAI YACGTR 1 cut(s) 61
BsaHI GRCGYC 2 cut(s) 483, 524
BsaWI WCCGGW 2 cut(s) 183, 566
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bse118I RCCGGY 1 cut(s) 183
Bse1I ACTGG 2 cut(s) 743, 748
Bse3DI GCAATG 1 cut(s) 810
BseBI CCWGG 1 cut(s) 1093
BseGI GGATG 3 cut(s) 297, 336, 1153
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 5 cut(s) 259, 372, 667, 810, 1029
BseNI ACTGG 2 cut(s) 743, 748
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 5 cut(s) 649, 655, 671, 677, 770
BsgI GTGCAG 1 cut(s) 678
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 2 cut(s) 161, 201
BshNI GGYRCC 1 cut(s) 82
BshTI ACCGGT 1 cut(s) 183
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 2 cut(s) 246, 274
BsiSI CCGG 3 cut(s) 184, 340, 567
BsmAI GTCTC 1 cut(s) 907
BsnI GGCC 2 cut(s) 161, 201
Bsp1286I GDGCHC 2 cut(s) 246, 274
Bsp143I GATC 6 cut(s) 138, 645, 676, 718, 815, 1017
BspACI CCGC 1 cut(s) 80
BspANI GGCC 2 cut(s) 161, 201
BspCNI CTCAG 5 cut(s) 258, 373, 666, 811, 1030
BspLI GGNNCC 3 cut(s) 84, 191, 1154
BspMAI CTGCAG 2 cut(s) 521, 667
BspPI GGATC 3 cut(s) 146, 653, 713
BspT107I GGYRCC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 810
BsrFI RCCGGY 1 cut(s) 183
BsrI ACTGG 2 cut(s) 743, 748
BssAI RCCGGY 1 cut(s) 183
BssMI GATC 6 cut(s) 138, 645, 676, 718, 815, 1017
BssNAI GTATAC 2 cut(s) 949, 1064
BssNI GRCGYC 2 cut(s) 483, 524
Bst1107I GTATAC 2 cut(s) 949, 1064
Bst2UI CCWGG 1 cut(s) 1093
Bst4CI ACNGT 2 cut(s) 134, 208
Bst6I CTCTTC 2 cut(s) 603, 1020
BstACI GRCGYC 2 cut(s) 483, 524
BstBAI YACGTR 1 cut(s) 61
BstC8I GCNNGC 2 cut(s) 855, 1044
BstDEI CTNAG 7 cut(s) 245, 381, 653, 723, 809, 819, 1038
BstF5I GGATG 3 cut(s) 297, 336, 1153
BstH2I RGCGCY 1 cut(s) 868
BstHHI GCGC 1 cut(s) 867
BstKTI GATC 6 cut(s) 141, 648, 679, 721, 818, 1020
BstMAI GTCTC 1 cut(s) 907
BstMBI GATC 6 cut(s) 138, 645, 676, 718, 815, 1017
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 3 cut(s) 665, 668, 758
BstNI CCWGG 1 cut(s) 1093
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 1091
BstSFI CTRYAG 4 cut(s) 207, 262, 517, 663
BstV1I GCAGC 5 cut(s) 649, 655, 671, 677, 770
BstV2I GAAGAC 1 cut(s) 995
BstX2I RGATCY 2 cut(s) 815, 1017
BstYI RGATCY 2 cut(s) 815, 1017
BstZ17I GTATAC 2 cut(s) 949, 1064
BsuRI GGCC 2 cut(s) 161, 201
BtsCI GGATG 3 cut(s) 297, 336, 1153
BtsIMutI CAGTG 1 cut(s) 661
Cac8I GCNNGC 2 cut(s) 855, 1044
CaiI CAGNNNCTG 1 cut(s) 785
CfoI GCGC 1 cut(s) 867
Cfr10I RCCGGY 1 cut(s) 183
Cfr13I GGNCC 1 cut(s) 190
CseI GACGC 2 cut(s) 491, 883
Csp6I GTAC 3 cut(s) 70, 250, 887
CspAI ACCGGT 1 cut(s) 183
CviAII CATG 7 cut(s) 349, 365, 377, 458, 490, 528, 575
CviQI GTAC 3 cut(s) 70, 250, 887
DdeI CTNAG 7 cut(s) 245, 381, 653, 723, 809, 819, 1038
DpnI GATC 6 cut(s) 140, 647, 678, 720, 817, 1019
DpnII GATC 6 cut(s) 138, 645, 676, 718, 815, 1017
DrdI GACNNNNNNGTC 2 cut(s) 188, 529
DseDI GACNNNNNNGTC 2 cut(s) 188, 529
Eam1104I CTCTTC 2 cut(s) 603, 1020
EarI CTCTTC 2 cut(s) 603, 1020
Ecl136II GAGCTC 2 cut(s) 244, 272
Eco147I AGGCCT 1 cut(s) 161
Eco24I GRGCYC 2 cut(s) 246, 274
Eco47I GGWCC 1 cut(s) 190
Eco53kI GAGCTC 2 cut(s) 244, 272
Eco57I CTGAAG 1 cut(s) 1137
Eco72I CACGTG 1 cut(s) 61
EcoICRI GAGCTC 2 cut(s) 244, 272
EcoRII CCWGG 1 cut(s) 1091
EcoT38I GRGCYC 2 cut(s) 246, 274
FaeI CATG 7 cut(s) 352, 368, 380, 461, 493, 531, 578
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FatI CATG 7 cut(s) 348, 364, 376, 457, 489, 527, 574
FauNDI CATATG 2 cut(s) 640, 859
FblI GTMKAC 2 cut(s) 948, 1063
Fnu4HI GCNGC 6 cut(s) 81, 660, 663, 666, 669, 759
FokI GGATG 3 cut(s) 304, 323, 1160
FriOI GRGCYC 2 cut(s) 246, 274
Fsp4HI GCNGC 6 cut(s) 81, 660, 663, 666, 669, 759
FspBI CTAG 1 cut(s) 890
GlaI GCGC 1 cut(s) 866
GluI GCNGC 6 cut(s) 81, 660, 663, 666, 669, 759
HaeII RGCGCY 1 cut(s) 868
HaeIII GGCC 2 cut(s) 161, 201
HapII CCGG 3 cut(s) 184, 340, 567
HgaI GACGC 2 cut(s) 491, 883
HhaI GCGC 1 cut(s) 867
Hin1I GRCGYC 2 cut(s) 483, 524
Hin1II CATG 7 cut(s) 352, 368, 380, 461, 493, 531, 578
Hin6I GCGC 1 cut(s) 865
HinP1I GCGC 1 cut(s) 865
HinfI GANTC 8 cut(s) 113, 175, 633, 651, 697, 713, 727, 1034
HpaII CCGG 3 cut(s) 184, 340, 567
Hpy166II GTNNAC 5 cut(s) 430, 736, 790, 949, 1064
Hpy188I TCNGA 9 cut(s) 99, 138, 328, 594, 650, 676, 718, 820, 1039
Hpy188III TCNNGA 6 cut(s) 106, 179, 275, 404, 985, 1106
Hpy8I GTNNAC 5 cut(s) 430, 736, 790, 949, 1064
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 3 cut(s) 103, 191, 1138
HpyCH4III ACNGT 2 cut(s) 134, 208
HpyCH4IV ACGT 3 cut(s) 60, 330, 524
HpyCH4V TGCA 8 cut(s) 356, 461, 493, 519, 659, 665, 758, 884
HpyF10VI GCNNNNNNNGC 3 cut(s) 665, 668, 758
HpyF3I CTNAG 7 cut(s) 245, 381, 653, 723, 809, 819, 1038
HpySE526I ACGT 3 cut(s) 60, 330, 524
Hsp92I GRCGYC 2 cut(s) 483, 524
Hsp92II CATG 7 cut(s) 352, 368, 380, 461, 493, 531, 578
HspAI GCGC 1 cut(s) 865
Kzo9I GATC 6 cut(s) 138, 645, 676, 718, 815, 1017
LmnI GCTCC 5 cut(s) 29, 147, 241, 782, 934
Lsp1109I GCAGC 5 cut(s) 649, 655, 671, 677, 770
LweI GCATC 1 cut(s) 627
MaeI CTAG 1 cut(s) 890
MaeII ACGT 3 cut(s) 60, 330, 524
MaeIII GTNAC 4 cut(s) 56, 226, 296, 926
MalI GATC 6 cut(s) 140, 647, 678, 720, 817, 1019
MboI GATC 6 cut(s) 138, 645, 676, 718, 815, 1017
MboII GAAGA 5 cut(s) 508, 620, 907, 1000, 1037
MfeI CAATTG 1 cut(s) 410
MflI RGATCY 2 cut(s) 815, 1017
MhlI GDGCHC 2 cut(s) 246, 274
MluCI AATT 5 cut(s) 152, 410, 550, 581, 687
MlyI GAGTC 3 cut(s) 107, 645, 736
MmeI TCCRAC 2 cut(s) 116, 450
MseI TTAA 2 cut(s) 156, 393
MspA1I CMGCKG 2 cut(s) 662, 668
MspI CCGG 3 cut(s) 184, 340, 567
MspR9I CCNGG 1 cut(s) 1093
MunI CAATTG 1 cut(s) 410
MvaI CCWGG 1 cut(s) 1093
MwoI GCNNNNNNNGC 3 cut(s) 665, 668, 758
NdeI CATATG 2 cut(s) 640, 859
NdeII GATC 6 cut(s) 138, 645, 676, 718, 815, 1017
NlaIII CATG 7 cut(s) 352, 368, 380, 461, 493, 531, 578
NlaIV GGNNCC 3 cut(s) 84, 191, 1154
NmuCI GTSAC 3 cut(s) 56, 226, 296
NspI RCATGY 1 cut(s) 380
PceI AGGCCT 1 cut(s) 161
PfeI GAWTC 5 cut(s) 175, 633, 697, 713, 1034
PflFI GACNNNGTC 1 cut(s) 224
PinAI ACCGGT 1 cut(s) 183
PkrI GCNGC 6 cut(s) 82, 661, 664, 667, 670, 760
PleI GAGTC 3 cut(s) 107, 645, 735
PmaCI CACGTG 1 cut(s) 61
PmlI CACGTG 1 cut(s) 61
PpsI GAGTC 3 cut(s) 107, 645, 735
Ppu21I YACGTR 1 cut(s) 61
PsiI TTATAA 1 cut(s) 561
Psp124BI GAGCTC 2 cut(s) 246, 274
Psp6I CCWGG 1 cut(s) 1091
PspCI CACGTG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 1091
PspN4I GGNNCC 3 cut(s) 84, 191, 1154
PspPI GGNCC 1 cut(s) 190
PsrI GAACNNNNNNTAC 2 cut(s) 113, 145
PstI CTGCAG 2 cut(s) 521, 667
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 2 cut(s) 815, 1017
PsyI GACNNNGTC 1 cut(s) 224
PvuII CAGCTG 2 cut(s) 662, 668
RsaI GTAC 3 cut(s) 71, 251, 888
RsaNI GTAC 3 cut(s) 70, 250, 887
SacI GAGCTC 2 cut(s) 246, 274
SaqAI TTAA 2 cut(s) 156, 393
SatI GCNGC 6 cut(s) 81, 660, 663, 666, 669, 759
Sau3AI GATC 6 cut(s) 138, 645, 676, 718, 815, 1017
Sau96I GGNCC 1 cut(s) 190
ScaI AGTACT 1 cut(s) 888
SchI GAGTC 3 cut(s) 107, 645, 736
ScrFI CCNGG 1 cut(s) 1093
SduI GDGCHC 2 cut(s) 246, 274
SfaNI GCATC 1 cut(s) 627
SfcI CTRYAG 4 cut(s) 207, 262, 517, 663
SinI GGWCC 1 cut(s) 190
SmlI CTYRAG 2 cut(s) 275, 680
SmoI CTYRAG 2 cut(s) 275, 680
Sse9I AATT 5 cut(s) 152, 410, 550, 581, 687
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 1 cut(s) 80
SspMI CTAG 1 cut(s) 890
SstI GAGCTC 2 cut(s) 246, 274
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 1 cut(s) 1091
TaaI ACNGT 2 cut(s) 134, 208
TaiI ACGT 3 cut(s) 63, 333, 527
TaqI TCGA 2 cut(s) 180, 345
TasI AATT 5 cut(s) 152, 410, 550, 581, 687
TatI WGTACW 2 cut(s) 69, 886
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 5 cut(s) 175, 633, 697, 713, 1034
Tru1I TTAA 2 cut(s) 156, 393
Tru9I TTAA 2 cut(s) 156, 393
TscAI CASTG 1 cut(s) 661
TseFI GTSAC 3 cut(s) 56, 226, 296
TseI GCWGC 5 cut(s) 659, 662, 665, 668, 758
Tsp45I GTSAC 3 cut(s) 56, 226, 296
TspDTI ATGAA 4 cut(s) 20, 353, 956, 1038
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 1 cut(s) 661
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 2 cut(s) 152, 581
XceI RCATGY 1 cut(s) 380
XmiI GTMKAC 2 cut(s) 948, 1063
XspI CTAG 1 cut(s) 890
ZraI GACGTC 1 cut(s) 525
ZrmI AGTACT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.