AT2G23142

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000986
Physical Location & Seq
Forward (+)
9850176 .. 9850619
444 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G23142.1

Sequence Viewer

Length: 444 bp
ATGAATTGTTTTTCTTATTTTTTCCTTGTTATTATATTATGTGCAGGATTGAATAATGCGAAATTCAACGAAAAAAACTCCGTAATTTTTAAGAGTTCTCTTGGTCCGAAAAAACTTCTCAGGATTCATTGTACATCAGAACACGACGACACCGACTACGTTTACCTGAGACATGGACAAACTTACGCATTCAGTTTTCATGATAGTGTCTTGAAAACTATATTTGATTGTGAGTTAAAGCAAGGAAGTAGTTATTATAATTACAACTTTTATGCAAGATTTAGGGCATATAAAGGTGGAGGTCTGATAGTTCACTACGGTAAAAAGAACTTTTGGGATGCTAGAGAAGATGGTATTTACTTCACACATGGTAAAGAAACACCTAAATTAGAGTATAAATGGATCCCCGGGGATCCCTCTATGAGAGTGGAGAGCCCCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

17.17

Weight (kDa)

8.8

Isoelectric Point (pI)

30.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 28 - 134 3.4e-25 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AclWI GGATC 4 cut(s) 397, 407, 410, 420
AcsI RAATTY 1 cut(s) 62
AfaI GTAC 1 cut(s) 133
AgsI TTSAA 3 cut(s) 52, 67, 214
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 4 cut(s) 397, 407, 410, 420
Ama87I CYCGRG 1 cut(s) 407
ApoI RAATTY 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 104
AsuC2I CCSGG 2 cut(s) 408, 409
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 1 cut(s) 104
BamHI GGATCC 2 cut(s) 402, 412
BanII GRGCYC 1 cut(s) 437
BccI CCATC 1 cut(s) 344
BcnI CCSGG 2 cut(s) 408, 409
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 342
Bme1390I CCNGG 2 cut(s) 408, 409
Bme18I GGWCC 1 cut(s) 104
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 104
BmiI GGNNCC 2 cut(s) 404, 414
BmrFI CCNGG 2 cut(s) 408, 409
BmsI GCATC 1 cut(s) 328
BpuMI CCSGG 2 cut(s) 408, 409
BsaJI CCNNGG 3 cut(s) 406, 407, 408
BseDI CCNNGG 3 cut(s) 406, 407, 408
BseGI GGATG 1 cut(s) 343
BseMII CTCAG 2 cut(s) 133, 158
BsgI GTGCAG 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 1 cut(s) 408
BsmAI GTCTC 1 cut(s) 163
BsmI GAATGC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 1 cut(s) 437
Bsp1407I TGTACA 1 cut(s) 131
Bsp143I GATC 2 cut(s) 402, 412
BspCNI CTCAG 2 cut(s) 132, 159
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 2 cut(s) 404, 414
BspPI GGATC 4 cut(s) 397, 407, 410, 420
BsrGI TGTACA 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 406, 407, 408
BssMI GATC 2 cut(s) 402, 412
Bst4CI ACNGT 1 cut(s) 320
BstAUI TGTACA 1 cut(s) 131
BstDEI CTNAG 2 cut(s) 119, 167
BstF5I GGATG 1 cut(s) 343
BstKTI GATC 2 cut(s) 405, 415
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 2 cut(s) 402, 412
BstSCI CCNGG 2 cut(s) 406, 407
BstX2I RGATCY 2 cut(s) 402, 412
BstYI RGATCY 2 cut(s) 402, 412
BtsCI GGATG 1 cut(s) 343
CciI TCATGA 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 104
Cfr9I CCCGGG 1 cut(s) 407
Csp6I GTAC 1 cut(s) 132
CviAII CATG 3 cut(s) 173, 200, 368
CviJI RGCY 1 cut(s) 435
CviKI_1 RGCY 1 cut(s) 435
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 2 cut(s) 119, 167
DpnI GATC 2 cut(s) 404, 414
DpnII GATC 2 cut(s) 402, 412
Eco24I GRGCYC 1 cut(s) 437
Eco47I GGWCC 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 407
EcoT38I GRGCYC 1 cut(s) 437
FaeI CATG 3 cut(s) 176, 203, 371
FatI CATG 3 cut(s) 172, 199, 367
FokI GGATG 1 cut(s) 350
FriOI GRGCYC 1 cut(s) 437
FspBI CTAG 1 cut(s) 342
HapII CCGG 1 cut(s) 408
Hin1II CATG 3 cut(s) 176, 203, 371
HinfI GANTC 1 cut(s) 124
HpaII CCGG 1 cut(s) 408
Hpy166II GTNNAC 2 cut(s) 163, 313
Hpy188I TCNGA 3 cut(s) 108, 139, 306
Hpy188III TCNNGA 3 cut(s) 121, 200, 211
Hpy8I GTNNAC 2 cut(s) 163, 313
Hpy99I CGWCG 1 cut(s) 149
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 2 cut(s) 44, 275
HpyF3I CTNAG 2 cut(s) 119, 167
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 3 cut(s) 176, 203, 371
Kzo9I GATC 2 cut(s) 402, 412
LpnPI CCDG 4 cut(s) 30, 106, 179, 421
LweI GCATC 1 cut(s) 328
MaeI CTAG 1 cut(s) 342
MaeII ACGT 1 cut(s) 159
MalI GATC 2 cut(s) 404, 414
MboI GATC 2 cut(s) 402, 412
MboII GAAGA 1 cut(s) 359
MflI RGATCY 2 cut(s) 402, 412
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 5 cut(s) 4, 62, 84, 259, 386
MnlI CCTC 2 cut(s) 293, 427
MseI TTAA 2 cut(s) 90, 236
MslI CAYNNNNRTG 1 cut(s) 204
MspI CCGG 1 cut(s) 408
MspR9I CCNGG 2 cut(s) 408, 409
Mva1269I GAATGC 1 cut(s) 188
NciI CCSGG 2 cut(s) 408, 409
NdeII GATC 2 cut(s) 402, 412
NlaIII CATG 3 cut(s) 176, 203, 371
NlaIV GGNNCC 2 cut(s) 404, 414
PagI TCATGA 1 cut(s) 199
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 1 cut(s) 188
PfeI GAWTC 1 cut(s) 124
PsiI TTATAA 1 cut(s) 258
PspN4I GGNNCC 2 cut(s) 404, 414
PspPI GGNCC 1 cut(s) 104
PsuI RGATCY 2 cut(s) 402, 412
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 1 cut(s) 204
SaqAI TTAA 2 cut(s) 90, 236
Sau3AI GATC 2 cut(s) 402, 412
Sau96I GGNCC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 408, 409
SduI GDGCHC 1 cut(s) 437
SetI ASST 5 cut(s) 162, 168, 298, 304, 385
SfaNI GCATC 1 cut(s) 328
SinI GGWCC 1 cut(s) 104
SmaI CCCGGG 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 204
Sse9I AATT 5 cut(s) 4, 62, 84, 259, 386
SspMI CTAG 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 406, 407
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 1 cut(s) 162
TasI AATT 5 cut(s) 4, 62, 84, 259, 386
TatI WGTACW 1 cut(s) 131
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 2 cut(s) 90, 236
Tru9I TTAA 2 cut(s) 90, 236
TspDTI ATGAA 3 cut(s) 17, 116, 188
TspGWI ACGGA 1 cut(s) 70
TspMI CCCGGG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 104
XapI RAATTY 1 cut(s) 62
XmaI CCCGGG 1 cut(s) 407
XspI CTAG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.