Rh5BG181300

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000986
Physical Location & Seq
Forward (+)
19897338 .. 19897673
336 bp
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UTR
Exon/CDS
Intron
Rh5BG181300.1

Sequence Viewer

Length: 336 bp
ATGAAGGGATCAAGAGCATCGTTCATGTTGTTCTTTGTTGGGTTGACATTTTGTCTTGCGAGCCCGGAGTCTACTGCTTCGACTCCGGGTGGAGAGAGTGACTGGTGGACACCTAGACCCGGTAAAAAGTATTTTGTGCGGATTGTGAACGACCTCAATAAACAGAAACTAGACTATCCTTATAAATATGTAGACGATGATCTCAGACTTCGTAGTCTTCCTCATCAAGCGCAGTACGAGTTTGGATTTAGGCTCAACTTCGCAAGTACCACACTTTTCACCTGCGATTTCACTCATGCTCTCAATTCTCAACCATGTCACCTTTGTAGCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

111

Amino Acids

12.74

Weight (kDa)

8.39

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 46 - 101 2.2e-08 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 183
AarI CACCTGC 1 cut(s) 290
AasI GACNNNNNNGTC 1 cut(s) 213
Acc36I ACCTGC 1 cut(s) 290
AccI GTMKAC 2 cut(s) 71, 192
AciI CCGC 1 cut(s) 139
AclWI GGATC 1 cut(s) 16
AfaI GTAC 2 cut(s) 236, 268
AfiI CCNNNNNNNGG 1 cut(s) 119
AhdI GACNNNNNGTC 1 cut(s) 51
AlwI GGATC 1 cut(s) 16
AspLEI GCGC 1 cut(s) 232
AsuC2I CCSGG 3 cut(s) 65, 87, 120
AsuHPI GGTGA 2 cut(s) 271, 311
BanII GRGCYC 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 209
BcnI CCSGG 3 cut(s) 65, 87, 120
BfaI CTAG 2 cut(s) 114, 170
BfuAI ACCTGC 1 cut(s) 290
Bme1390I CCNGG 3 cut(s) 65, 87, 120
BmeRI GACNNNNNGTC 1 cut(s) 51
BmrFI CCNGG 3 cut(s) 65, 87, 120
BmsI GCATC 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 209
BpuMI CCSGG 3 cut(s) 65, 87, 120
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 107
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMII CTCAG 1 cut(s) 217
BseNI ACTGG 1 cut(s) 107
BsiSI CCGG 3 cut(s) 65, 86, 120
BslI CCNNNNNNNGG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 2 cut(s) 8, 199
BspACI CCGC 1 cut(s) 139
BspCNI CTCAG 1 cut(s) 216
BspMI ACCTGC 1 cut(s) 290
BspPI GGATC 1 cut(s) 16
BsrI ACTGG 1 cut(s) 107
BssMI GATC 2 cut(s) 8, 199
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 1 cut(s) 203
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 2 cut(s) 11, 202
BstMBI GATC 2 cut(s) 8, 199
BstSCI CCNGG 3 cut(s) 63, 85, 118
BstV2I GAAGAC 1 cut(s) 209
BveI ACCTGC 1 cut(s) 290
Cac8I GCNNGC 1 cut(s) 61
CfoI GCGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 235, 267
CviAII CATG 3 cut(s) 25, 296, 315
CviJI RGCY 2 cut(s) 63, 253
CviKI_1 RGCY 2 cut(s) 63, 253
CviQI GTAC 2 cut(s) 235, 267
DdeI CTNAG 1 cut(s) 203
DpnI GATC 2 cut(s) 10, 201
DpnII GATC 2 cut(s) 8, 199
DrdI GACNNNNNNGTC 1 cut(s) 213
DriI GACNNNNNGTC 1 cut(s) 51
DseDI GACNNNNNNGTC 1 cut(s) 213
Eam1105I GACNNNNNGTC 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 65
EcoT38I GRGCYC 1 cut(s) 65
FaeI CATG 3 cut(s) 28, 299, 318
FaiI YATR 5 cut(s) 26, 183, 189, 297, 316
FatI CATG 3 cut(s) 24, 295, 314
FblI GTMKAC 2 cut(s) 71, 192
FriOI GRGCYC 1 cut(s) 65
FspBI CTAG 2 cut(s) 114, 170
GlaI GCGC 1 cut(s) 231
HapII CCGG 3 cut(s) 65, 86, 120
HhaI GCGC 1 cut(s) 232
Hin1II CATG 3 cut(s) 28, 299, 318
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 2 cut(s) 68, 82
HpaII CCGG 3 cut(s) 65, 86, 120
HphI GGTGA 2 cut(s) 271, 311
Hpy166II GTNNAC 5 cut(s) 45, 72, 108, 148, 193
Hpy188I TCNGA 1 cut(s) 206
Hpy188III TCNNGA 1 cut(s) 12
Hpy8I GTNNAC 5 cut(s) 45, 72, 108, 148, 193
HpyF3I CTNAG 1 cut(s) 203
Hsp92II CATG 3 cut(s) 28, 299, 318
HspAI GCGC 1 cut(s) 230
Kzo9I GATC 2 cut(s) 8, 199
LpnPI CCDG 5 cut(s) 78, 88, 99, 133, 295
LweI GCATC 1 cut(s) 26
MaeI CTAG 2 cut(s) 114, 170
MaeIII GTNAC 2 cut(s) 98, 317
MalI GATC 2 cut(s) 10, 201
MboI GATC 2 cut(s) 8, 199
MboII GAAGA 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 1 cut(s) 304
MlyI GAGTC 2 cut(s) 76, 77
MnlI CCTC 2 cut(s) 164, 231
MspI CCGG 3 cut(s) 65, 86, 120
MspR9I CCNGG 3 cut(s) 65, 87, 120
NciI CCSGG 3 cut(s) 65, 87, 120
NdeII GATC 2 cut(s) 8, 199
NlaIII CATG 3 cut(s) 28, 299, 318
NmuCI GTSAC 2 cut(s) 98, 317
PaqCI CACCTGC 1 cut(s) 290
PleI GAGTC 2 cut(s) 76, 76
PpsI GAGTC 2 cut(s) 76, 76
PsiI TTATAA 1 cut(s) 183
RsaI GTAC 2 cut(s) 236, 268
RsaNI GTAC 2 cut(s) 235, 267
Sau3AI GATC 2 cut(s) 8, 199
SchI GAGTC 2 cut(s) 76, 77
ScrFI CCNGG 3 cut(s) 65, 87, 120
SduI GDGCHC 1 cut(s) 65
SetI ASST 4 cut(s) 115, 156, 284, 324
SfaNI GCATC 1 cut(s) 26
Sse9I AATT 1 cut(s) 304
SsiI CCGC 1 cut(s) 139
SspMI CTAG 2 cut(s) 114, 170
StyD4I CCNGG 3 cut(s) 63, 85, 118
TaqI TCGA 1 cut(s) 80
TasI AATT 1 cut(s) 304
TseFI GTSAC 2 cut(s) 98, 317
Tsp45I GTSAC 2 cut(s) 98, 317
TspDTI ATGAA 2 cut(s) 13, 17
XmiI GTMKAC 2 cut(s) 71, 192
XspI CTAG 2 cut(s) 114, 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.