AT5G35715

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000354
Physical Location & Seq
Forward (+)
13881222 .. 13882937
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35715.1

Sequence Viewer

Length: 1329 bp
ATGCTTCTCCGTTTCGGGGTCGTCCCGATGGTTGTTATCTCTTCCAAGGAAGCAGCTAAACAAGTTCTAAAGTCTCGCGATCTAGAGACATGCTCCCGACCTAAGCTGGTTGCGAACGGGTTGTTTACTCGCAACTTCAAAGACATTGCTTTCGCTCAGTATGGTGAGGACTGGCGAGAGATGAAAAAGCTCGTGGGGCTTGAGCTCTTCAATCCAAAGAAGCACAAATTTTTCAGGTATATCAGAGAGGAAGAGGGTGACTTGCTGGTCAAGAAACTATCCAAATCTTCTCAAACACAAACTTTAGTGGATTTGAGAAAAGCCTTTTTCTCCTTCACCGCGGGTATCATATTTAGAGTCTCCTTTGGACAGAACTTCCGCGAGTGCGATTTCATCGATATGGACAGACTTGAAGAGTTGGTGCAAGAGTCAGAGACCAACGTATTCAGCTTCGCATTCACTGACTTCTTCCCAACGGGTCTCGGTTGGCTTGTAGACCGGATCTCCGGTCAACATTCGAGGATCGAGAAAGCCTTTTCAAAACTCACCAAGTTCTTTCAACATGTAATCGATGAGGAGTTGAAGATTGGACAATCCCAAGATCACTCAAACCTCGTCAGTTCCATGTTGGATATGATCAATAGATCCACTGAATACGGTTCTTTCAAGATCACTTCTGATCATCTCATAGCAATGATGACGGACATAGTTTTGGGGGGAGTGAACGCAGGAACAATCACAATGATATGGACGATGACAGAGCTAACCAGACATCCAAGAGTGATGAAGAAACTCCGAGAAGAGATTCGAGCAACACTTGGACCCAACAAAGAGAGAATCACAGAAGAAGATCTAGAGAAAGTTGAGTACTTGAAGTTGGTGATCAAAGAAACTTTCAGATTACATCCACCAGGTCCTTTCTTGCTCCCAAGACAAGTAATGTCCGACATAGAGATTCAAGGCTACCACATTCCCAAGAACGCACATATCAAGATCAGCACTTACGCCATAGGACGTGATCCCAAATGTTGGACTAATCCTGAAGAATTCAATCCTGAGAGGTTTGCAAATACCTCAATCAACTACAAAGGTCAGCATTACGAGCTTTTACCCTTTGGAGCCGGTCGTAGGAGCTGTCCTGGTATGACTTTGGGAATCACCATTCTTGAGCTAGGCCTTCTTAACATTCTTTACTACTTCGATTGGAGTTTACCCAATGGAATGACCATTAAAGACATTGACATGGAAGAAGATGGAGCTTTGACCATCGCCAAGAAAGTCCCTCTTGAGCTCATACCTACGCTCCCAGCATCACTATGCATTAAATAA

Protein Analysis

442

Amino Acids

50.74

Weight (kDa)

7.66

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 426 4.5e-93 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13080 AT1G13080 AT1G13090 AT1G13090 AT1G13100 AT1G13110 AT2G02580 AT2G24180 AT3G26150 AT3G26160 AT3G26170 AT3G26180 AT3G26180 AT3G26190 AT3G26200 AT3G26210 AT3G26290 AT3G26290 AT3G26300 AT3G26310 AT3G26320 AT3G26330 AT3G26830 AT3G44250 AT3G53280 AT3G53290 AT3G53300 AT3G53305 AT5G25120 AT5G25130 AT5G25140 AT5G25180 AT5G35715 AT5G57260
fragaria_vesca FvH4_4g25390 FvH4_4g25390 FvH4_4g25390
malus_domestica MD13G1103500.v1.1 MD13G1103800.v1.1 MD13G1104100.v1.1 MD16G1103600.v1.1 MD16G1104000.v1.1 MD16G1104200.v1.1 MD16G1104300.v1.1
prunus_persica Prupe.1G170800_v2.0.a1 Prupe.1G242300_v2.0.a1 Prupe.1G242400_v2.0.a1
pyrus_communis pycom13g09040 pycom16g08850 pycom16g08860
rosa_chinensis RchiOBHm_Chr4g0419321 RchiOBHm_Chr4g0419411 RchiOBHm_Chr4g0419461 RchiOBHm_Chr4g0419481 RchiOBHm_Chr4g0419491 RchiOBHm_Chr4g0419571 RchiOBHm_Chr4g0432681 RchiOBHm_Chr4g0432701 RchiOBHm_Chr5g0025811
rosa_laevigata RLG00000003494 RLG00000006819 RLG00000006820 RLG00000006821 RLG00000006823 RLG00000007814 RLG00000007815 RLG00000032878
rosa_multiflora Rmu_co8315439.1_g000001 Rmu_sc0000455.1_g000013 Rmu_sc0000455.1_g000035 Rmu_sc0000455.1_g000058 Rmu_sc0000675.1_g000043 Rmu_sc0001912.1_g000035 Rmu_sc0002401.1_g000014 Rmu_sc0004932.1_g000013 Rmu_sc0008245.1_g000007 Rmu_sc0009481.1_g000005 Rmu_ssc0000467.1_g000029
rosa_roxburghii Rroxscaffold_1G00053390 Rroxscaffold_1G00054350 Rroxscaffold_4G00324380 Rroxscaffold_5G00362300 Rroxscaffold_5G00362360 Rroxscaffold_5G00374140 Rroxscaffold_5G00374160
rosa_rugosa Rorug04G0158300 Rorug04G0261600 Rorug04G0261700 Rorug04G0261800
rosa_samantha Rh4AG220300 Rh4AG316100 Rh4BG221600 Rh4BG222000 Rh4BG222100 Rh4BG222200 Rh4BG324100 Rh4CG233000 Rh4CG233100 Rh4CG233200 Rh4CG233900 Rh4CG339100 Rh4DG319500
rosa_wichuraiana Rw4G018840 Rw4G018880 Rw4G018900 Rw4G018930 Rw4G027500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
AccB7I CCANNNNNTGG 1 cut(s) 1029
AccI GTMKAC 1 cut(s) 495
AccII CGCG 3 cut(s) 78, 341, 381
AciI CCGC 3 cut(s) 339, 341, 379
AclWI GGATC 4 cut(s) 509, 530, 639, 1013
AcsI RAATTY 2 cut(s) 227, 1046
AcuI CTGAAG 1 cut(s) 1062
AfaI GTAC 1 cut(s) 869
AfiI CCNNNNNNNGG 3 cut(s) 16, 1029, 1128
AflIII ACRYGT 1 cut(s) 562
AjiI CACGTC 1 cut(s) 1016
AjnI CCWGG 2 cut(s) 910, 1138
Alw21I GWGCWC 2 cut(s) 207, 1293
Alw26I GTCTC 5 cut(s) 78, 80, 364, 428, 485
AlwI GGATC 4 cut(s) 509, 530, 639, 1013
AoxI GGCC 1 cut(s) 1174
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 2 cut(s) 227, 1046
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 1 cut(s) 804
AspS9I GGNCC 2 cut(s) 821, 914
AsuHPI GGTGA 6 cut(s) 176, 269, 328, 538, 892, 1150
AvaII GGWCC 2 cut(s) 821, 914
BanII GRGCYC 2 cut(s) 207, 1293
BauI CACGAG 1 cut(s) 191
Bbv12I GWGCWC 2 cut(s) 207, 1293
BbvI GCAGC 1 cut(s) 65
BccI CCATC 3 cut(s) 22, 1247, 1274
BciT130I CCWGG 2 cut(s) 912, 1140
BclI TGATCA 3 cut(s) 636, 679, 882
BcoDI GTCTC 5 cut(s) 78, 80, 364, 428, 485
BfaI CTAG 3 cut(s) 83, 854, 1172
BglII AGATCT 1 cut(s) 850
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
BmcAI AGTACT 1 cut(s) 869
Bme1390I CCNGG 2 cut(s) 912, 1140
Bme18I GGWCC 2 cut(s) 821, 914
BmgBI CACGTC 1 cut(s) 1016
BmgT120I GGNCC 2 cut(s) 821, 914
BmiI GGNNCC 2 cut(s) 823, 1120
BmrFI CCNGG 2 cut(s) 912, 1140
BmsI GCATC 1 cut(s) 1319
BplI GAGNNNNNCTC 2 cut(s) 77, 109
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 3 cut(s) 221, 1187, 1307
Bsa29I ATCGAT 2 cut(s) 396, 570
BsaI GGTCTC 2 cut(s) 428, 485
BsaJI CCNNGG 2 cut(s) 45, 339
BsaWI WCCGGW 2 cut(s) 498, 506
BsaXI ACNNNNNCTCC 4 cut(s) 488, 518, 1287, 1317
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 1029, 1128
Bse118I RCCGGY 1 cut(s) 1121
Bse1I ACTGG 1 cut(s) 176
Bse3DI GCAATG 2 cut(s) 144, 699
BseBI CCWGG 2 cut(s) 912, 1140
BseCI ATCGAT 2 cut(s) 396, 570
BseDI CCNNGG 2 cut(s) 45, 339
BseGI GGATG 2 cut(s) 772, 904
BseLI CCNNNNNNNGG 3 cut(s) 16, 1029, 1128
BseMI GCAATG 2 cut(s) 144, 699
BseMII CTCAG 2 cut(s) 170, 1047
BseNI ACTGG 1 cut(s) 176
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 1 cut(s) 65
BseYI CCCAGC 1 cut(s) 1306
Bsh1236I CGCG 3 cut(s) 78, 341, 381
Bsh1285I CGRYCG 1 cut(s) 1126
BshFI GGCC 1 cut(s) 1176
BshVI ATCGAT 2 cut(s) 396, 570
BsiEI CGRYCG 1 cut(s) 1126
BsiHKAI GWGCWC 2 cut(s) 207, 1293
BsiSI CCGG 3 cut(s) 499, 507, 1122
BslFI GGGAC 2 cut(s) 8, 1265
BslI CCNNNNNNNGG 3 cut(s) 16, 1029, 1128
BsmAI GTCTC 5 cut(s) 78, 80, 364, 428, 485
BsmFI GGGAC 2 cut(s) 8, 1265
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 1 cut(s) 1176
Bso31I GGTCTC 2 cut(s) 428, 485
Bsp1286I GDGCHC 2 cut(s) 207, 1293
Bsp68I TCGCGA 1 cut(s) 78
BspACI CCGC 3 cut(s) 339, 341, 379
BspANI GGCC 1 cut(s) 1176
BspCNI CTCAG 2 cut(s) 169, 1048
BspDI ATCGAT 2 cut(s) 396, 570
BspFNI CGCG 3 cut(s) 78, 341, 381
BspLI GGNNCC 2 cut(s) 823, 1120
BspPI GGATC 4 cut(s) 509, 530, 639, 1013
BspQI GCTCTTC 1 cut(s) 212
BspTNI GGTCTC 2 cut(s) 428, 485
BsrDI GCAATG 2 cut(s) 144, 699
BsrFI RCCGGY 1 cut(s) 1121
BsrI ACTGG 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 1121
BssECI CCNNGG 2 cut(s) 45, 339
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 45
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 2 cut(s) 912, 1140
Bst4CI ACNGT 1 cut(s) 659
Bst6I CTCTTC 5 cut(s) 46, 212, 246, 408, 795
BstDEI CTNAG 3 cut(s) 102, 156, 1056
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 2 cut(s) 772, 904
BstFNI CGCG 3 cut(s) 78, 341, 381
BstMAI GTCTC 5 cut(s) 78, 80, 364, 428, 485
BstMCI CGRYCG 1 cut(s) 1126
BstMWI GCNNNNNNNGC 2 cut(s) 196, 1102
BstNI CCWGG 2 cut(s) 912, 1140
BstNSI RCATGY 2 cut(s) 93, 566
BstSCI CCNGG 2 cut(s) 910, 1138
BstUI CGCG 3 cut(s) 78, 341, 381
BstV1I GCAGC 1 cut(s) 65
BstX2I RGATCY 3 cut(s) 501, 644, 850
BstYI RGATCY 3 cut(s) 501, 644, 850
Bsu15I ATCGAT 2 cut(s) 396, 570
BsuRI GGCC 1 cut(s) 1176
BsuTUI ATCGAT 2 cut(s) 396, 570
BtgI CCRYGG 1 cut(s) 339
BtgZI GCGATG 1 cut(s) 1252
BtrI CACGTC 1 cut(s) 1016
BtsCI GGATG 2 cut(s) 772, 904
BtsIMutI CAGTG 2 cut(s) 459, 648
BtuMI TCGCGA 1 cut(s) 78
Cfr10I RCCGGY 1 cut(s) 1121
Cfr13I GGNCC 2 cut(s) 821, 914
Cfr42I CCGCGG 1 cut(s) 342
ClaI ATCGAT 2 cut(s) 396, 570
CsiI ACCWGGT 1 cut(s) 910
Csp6I GTAC 1 cut(s) 868
CviAII CATG 4 cut(s) 90, 563, 625, 1243
CviQI GTAC 1 cut(s) 868
DdeI CTNAG 3 cut(s) 102, 156, 1056
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
Eam1104I CTCTTC 5 cut(s) 46, 212, 246, 408, 795
EarI CTCTTC 5 cut(s) 46, 212, 246, 408, 795
Ecl136II GAGCTC 2 cut(s) 205, 1291
Eco130I CCWWGG 1 cut(s) 45
Eco147I AGGCCT 1 cut(s) 1176
Eco24I GRGCYC 2 cut(s) 207, 1293
Eco31I GGTCTC 2 cut(s) 428, 485
Eco47I GGWCC 2 cut(s) 821, 914
Eco53kI GAGCTC 2 cut(s) 205, 1291
Eco57I CTGAAG 1 cut(s) 1062
EcoICRI GAGCTC 2 cut(s) 205, 1291
EcoO109I RGGNCCY 1 cut(s) 914
EcoRI GAATTC 1 cut(s) 1046
EcoRII CCWGG 2 cut(s) 910, 1138
EcoT14I CCWWGG 1 cut(s) 45
EcoT22I ATGCAT 1 cut(s) 1322
EcoT38I GRGCYC 2 cut(s) 207, 1293
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 4 cut(s) 93, 566, 628, 1246
FalI AAGNNNNNCTT 2 cut(s) 1269, 1301
FaqI GGGAC 2 cut(s) 8, 1265
FatI CATG 4 cut(s) 89, 562, 624, 1242
FauI CCCGC 1 cut(s) 334
FbaI TGATCA 3 cut(s) 636, 679, 882
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 2 cut(s) 759, 891
FriOI GRGCYC 2 cut(s) 207, 1293
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 3 cut(s) 83, 854, 1172
GluI GCNGC 1 cut(s) 54
GsaI CCCAGC 1 cut(s) 1310
HaeIII GGCC 1 cut(s) 1176
HapII CCGG 3 cut(s) 499, 507, 1122
Hin1II CATG 4 cut(s) 93, 566, 628, 1246
HincII GTYRAC 1 cut(s) 512
HindII GTYRAC 1 cut(s) 512
HinfI GANTC 6 cut(s) 357, 428, 805, 837, 955, 1155
HpaII CCGG 3 cut(s) 499, 507, 1122
HphI GGTGA 6 cut(s) 176, 269, 328, 538, 892, 1150
Hpy166II GTNNAC 5 cut(s) 126, 496, 512, 724, 1211
Hpy188I TCNGA 6 cut(s) 245, 433, 679, 797, 899, 946
Hpy8I GTNNAC 5 cut(s) 126, 496, 512, 724, 1211
HpyAV CCTTC 2 cut(s) 343, 1187
HpyCH4III ACNGT 1 cut(s) 659
HpyCH4IV ACGT 2 cut(s) 441, 1015
HpyCH4V TGCA 3 cut(s) 424, 1067, 1320
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 1102
HpyF3I CTNAG 3 cut(s) 102, 156, 1056
HpySE526I ACGT 2 cut(s) 441, 1015
Hsp92II CATG 4 cut(s) 93, 566, 628, 1246
Ksp22I TGATCA 3 cut(s) 636, 679, 882
KspI CCGCGG 1 cut(s) 342
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 6 cut(s) 98, 930, 1118, 1131, 1256, 1308
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 1 cut(s) 1319
MabI ACCWGGT 1 cut(s) 910
MaeI CTAG 3 cut(s) 83, 854, 1172
MaeII ACGT 2 cut(s) 441, 1015
MaeIII GTNAC 1 cut(s) 257
MflI RGATCY 3 cut(s) 501, 644, 850
MhlI GDGCHC 2 cut(s) 207, 1293
MluCI AATT 2 cut(s) 227, 1046
MlyI GAGTC 2 cut(s) 366, 437
MmeI TCCRAC 3 cut(s) 609, 969, 1010
MnlI CCTC 9 cut(s) 160, 241, 247, 513, 568, 623, 1053, 1084, 1293
Mph1103I ATGCAT 1 cut(s) 1322
MroXI GAANNNNTTC 1 cut(s) 804
MseI TTAA 3 cut(s) 1182, 1230, 1323
MslI CAYNNNNRTG 4 cut(s) 398, 692, 1241, 1315
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 3 cut(s) 499, 507, 1122
MspR9I CCNGG 2 cut(s) 912, 1140
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 2 cut(s) 912, 1140
MvnI CGCG 3 cut(s) 78, 341, 381
MwoI GCNNNNNNNGC 2 cut(s) 196, 1102
NlaIII CATG 4 cut(s) 93, 566, 628, 1246
NlaIV GGNNCC 2 cut(s) 823, 1120
NmuCI GTSAC 1 cut(s) 257
NruI TCGCGA 1 cut(s) 78
NsiI ATGCAT 1 cut(s) 1322
NspI RCATGY 2 cut(s) 93, 566
PceI AGGCCT 1 cut(s) 1176
PciI ACATGT 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 212
PctI GAATGC 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 804
PfeI GAWTC 4 cut(s) 805, 837, 955, 1155
PflMI CCANNNNNTGG 1 cut(s) 1029
PkrI GCNGC 1 cut(s) 55
PleI GAGTC 2 cut(s) 365, 436
PpsI GAGTC 2 cut(s) 365, 436
PpuMI RGGWCCY 1 cut(s) 914
PscI ACATGT 1 cut(s) 562
Psp124BI GAGCTC 2 cut(s) 207, 1293
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 2 cut(s) 910, 1138
PspFI CCCAGC 1 cut(s) 1306
PspGI CCWGG 2 cut(s) 910, 1138
PspN4I GGNNCC 2 cut(s) 823, 1120
PspPI GGNCC 2 cut(s) 821, 914
PspPPI RGGWCCY 1 cut(s) 914
PsuI RGATCY 3 cut(s) 501, 644, 850
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 1 cut(s) 869
RsaNI GTAC 1 cut(s) 868
RseI CAYNNNNRTG 4 cut(s) 398, 692, 1241, 1315
SacI GAGCTC 2 cut(s) 207, 1293
SacII CCGCGG 1 cut(s) 342
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 3 cut(s) 1182, 1230, 1323
SatI GCNGC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 821, 914
ScaI AGTACT 1 cut(s) 869
SchI GAGTC 2 cut(s) 366, 437
ScrFI CCNGG 2 cut(s) 912, 1140
SduI GDGCHC 2 cut(s) 207, 1293
SexAI ACCWGGT 1 cut(s) 910
SfaNI GCATC 1 cut(s) 1319
Sfr303I CCGCGG 1 cut(s) 342
SgrBI CCGCGG 1 cut(s) 342
SinI GGWCC 2 cut(s) 821, 914
SmiMI CAYNNNNRTG 4 cut(s) 398, 692, 1241, 1315
SmlI CTYRAG 3 cut(s) 200, 1166, 1286
SmoI CTYRAG 3 cut(s) 200, 1166, 1286
Sse9I AATT 2 cut(s) 227, 1046
SseBI AGGCCT 1 cut(s) 1176
SsiI CCGC 3 cut(s) 339, 341, 379
SspMI CTAG 3 cut(s) 83, 854, 1172
SstI GAGCTC 2 cut(s) 207, 1293
StuI AGGCCT 1 cut(s) 1176
StyD4I CCNGG 2 cut(s) 910, 1138
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 659
TaiI ACGT 2 cut(s) 444, 1018
TaqI TCGA 6 cut(s) 396, 518, 525, 570, 808, 1200
TasI AATT 2 cut(s) 227, 1046
TatI WGTACW 1 cut(s) 867
TfiI GAWTC 4 cut(s) 805, 837, 955, 1155
Tru1I TTAA 3 cut(s) 1182, 1230, 1323
Tru9I TTAA 3 cut(s) 1182, 1230, 1323
TscAI CASTG 2 cut(s) 466, 655
TseFI GTSAC 1 cut(s) 257
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 3 cut(s) 197, 382, 800
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 2 cut(s) 466, 655
Van91I CCANNNNNTGG 1 cut(s) 1029
VpaK11BI GGWCC 2 cut(s) 821, 914
XapI RAATTY 2 cut(s) 227, 1046
XbaI TCTAGA 2 cut(s) 82, 853
XceI RCATGY 2 cut(s) 93, 566
XmiI GTMKAC 1 cut(s) 495
XmnI GAANNNNTTC 1 cut(s) 804
XspI CTAG 3 cut(s) 83, 854, 1172
ZrmI AGTACT 1 cut(s) 869
Zsp2I ATGCAT 1 cut(s) 1322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.