Rroxscaffold_5G00374140

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000354
Physical Location & Seq
Reverse (-)
55200106 .. 55202402
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00374140.1

Sequence Viewer

Length: 1371 bp
ATGGCTCTCAACACCCTACCGCTAACCTTATGGCTTCCATTTCTCCTTCTTCTCCCTCTACTAATTTTACTGAAAAAGAATAAAAAGCCACAAAAACACTTCCCGCCAAGCCCTCCTAAGCTTCCCATCATAGGCAACTTACACCAATTTGCACAATCTGGTTCATCACCTCACCAAAATCTATGGCGCCTCTCGAAAACGTTTGGTCCAATCATGCTCCTACGCCTCGGTCGCATACCGACCCTCATAATCTCATCAGCTGAAGCAGCAAAACAGGTCTTGAAAGATAATGATCTCAACTGCTGCAGCAGACCCTCCTCCTCGGGGTCTCGAAAACTCACCTACAACTATATAGACATGGGATTTGCGCCTTATGGTGAGTATTGGCGAGAAATGAGAAAACTATGTGTGCTTGAGCTTTTCAGTATGAAAAGGGTGCAGTCTTATCGGTTGATTAGGGAAGAAGAAGTGGCTAAAATGATTGACTCAATATCCAAAGAATCTGCATCTTCGTCCTCTAATTCATGTGTTAATATTACTGAGAAGTTGTATGCTGTCATGGGCAGTATAACTTTCAGGGTTATTTTCGGGACAAGTTTCGAGGGAAGTGATTTCGAGCACGATAGGTTTCATCTAGTGCTTCATGAAGCTGAAATCATGCTTGCAGGCTTCTCTGCAGCTGATTATTTTCCTTCTATAATTGGGTATCTTATAGACAGGGTCTCCGGGAAACATAAACAATTTGAAAGGGTATCCGGGGAACTTCATCACTTTTTTCAGCAGGTGATTGATGATCATCTCAAGCCAGGGAGGACACAGCCAGAGCATGAAGACATAGTTGATGTGTTGCTTAAAATAGTGAAGGAGCAAACTGGTTTTGGAGCTGCTCAGTTTGGTCATGATAACATCAAGGCACTTCTTGTGAACTTATTTCTAGGTGGAATAGATACTGGTGCAATTACCATGCTGTGGGCGATGGCTGAGCTAGCTAAGAAGCCTAGACTGATGAAGAAAGCACAGGATGAAGTTAGAAGCTGCGTTGGAAACAAAGGAAAAGTCACTGAAAGTGATACGGAACAGCTTTCGTACCTCAAGATGATAATCAAAGAAACACTTCGACTGCATCCTCCAGCTCCCCTGATGCTTCCAAGAGAGTCCATGTCCCCCTTTAAAGTCCTTGGGTATGATGTTGAACCCAAAACATTAATCATAATTAATGACTGGGTAATTTCACGAGATCCAGAATTTTGGAAAGACCCAGAAGAGTTCATCCCGGAAAGGTTTGATGATAGCTCTGTCGATTTTAGGGGGCAACACTTTGAGTTCTTGCCATTTGGAGCTGCTTTGATTGGAAATTGCCAGAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

456

Amino Acids

51.82

Weight (kDa)

7.68

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 447 3.7e-84 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13080 AT1G13080 AT1G13090 AT1G13090 AT1G13100 AT1G13110 AT2G02580 AT2G24180 AT3G26150 AT3G26160 AT3G26170 AT3G26180 AT3G26180 AT3G26190 AT3G26200 AT3G26210 AT3G26290 AT3G26290 AT3G26300 AT3G26310 AT3G26320 AT3G26330 AT3G26830 AT3G44250 AT3G53280 AT3G53290 AT3G53300 AT3G53305 AT5G25120 AT5G25130 AT5G25140 AT5G25180 AT5G35715 AT5G57260
fragaria_vesca FvH4_4g25390 FvH4_4g25390 FvH4_4g25390
malus_domestica MD13G1103500.v1.1 MD13G1103800.v1.1 MD13G1104100.v1.1 MD16G1103600.v1.1 MD16G1104000.v1.1 MD16G1104200.v1.1 MD16G1104300.v1.1
prunus_persica Prupe.1G170800_v2.0.a1 Prupe.1G242300_v2.0.a1 Prupe.1G242400_v2.0.a1
pyrus_communis pycom13g09040 pycom16g08850 pycom16g08860
rosa_chinensis RchiOBHm_Chr4g0419321 RchiOBHm_Chr4g0419411 RchiOBHm_Chr4g0419461 RchiOBHm_Chr4g0419481 RchiOBHm_Chr4g0419491 RchiOBHm_Chr4g0419571 RchiOBHm_Chr4g0432681 RchiOBHm_Chr4g0432701 RchiOBHm_Chr5g0025811
rosa_laevigata RLG00000003494 RLG00000006819 RLG00000006820 RLG00000006821 RLG00000006823 RLG00000007814 RLG00000007815 RLG00000032878
rosa_multiflora Rmu_co8315439.1_g000001 Rmu_sc0000455.1_g000013 Rmu_sc0000455.1_g000035 Rmu_sc0000455.1_g000058 Rmu_sc0000675.1_g000043 Rmu_sc0001912.1_g000035 Rmu_sc0002401.1_g000014 Rmu_sc0004932.1_g000013 Rmu_sc0008245.1_g000007 Rmu_sc0009481.1_g000005 Rmu_ssc0000467.1_g000029
rosa_roxburghii Rroxscaffold_1G00053390 Rroxscaffold_1G00054350 Rroxscaffold_4G00324380 Rroxscaffold_5G00362300 Rroxscaffold_5G00362360 Rroxscaffold_5G00374140 Rroxscaffold_5G00374160
rosa_rugosa Rorug04G0158300 Rorug04G0261600 Rorug04G0261700 Rorug04G0261800
rosa_samantha Rh4AG220300 Rh4AG316100 Rh4BG221600 Rh4BG222000 Rh4BG222100 Rh4BG222200 Rh4BG324100 Rh4CG233000 Rh4CG233100 Rh4CG233200 Rh4CG233900 Rh4CG339100 Rh4DG319500
rosa_wichuraiana Rw4G018840 Rw4G018880 Rw4G018900 Rw4G018930 Rw4G027500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 772
Acc36I ACCTGC 1 cut(s) 772
AccB1I GGYRCC 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 969
AciI CCGC 2 cut(s) 20, 104
AclI AACGTT 1 cut(s) 200
AclWI GGATC 1 cut(s) 1232
AcsI RAATTY 1 cut(s) 1244
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 1 cut(s) 1088
AfiI CCNNNNNNNGG 3 cut(s) 131, 324, 969
AgsI TTSAA 3 cut(s) 283, 746, 1193
AjnI CCWGG 1 cut(s) 805
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 2 cut(s) 333, 727
AlwI GGATC 1 cut(s) 1232
Ama87I CYCGRG 1 cut(s) 322
ApeKI GCWGC 7 cut(s) 266, 303, 306, 677, 884, 1035, 1340
ApoI RAATTY 1 cut(s) 1244
ArsI GACNNNNNNTTYG 4 cut(s) 347, 379, 1041, 1073
AseI ATTAAT 2 cut(s) 1205, 1215
Asp700I GAANNNNTTC 1 cut(s) 1113
AspLEI GCGC 2 cut(s) 189, 370
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 3 cut(s) 727, 757, 1274
AsuHPI GGTGA 5 cut(s) 159, 164, 331, 389, 796
AsuNHI GCTAGC 1 cut(s) 985
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 1 cut(s) 206
BanI GGYRCC 1 cut(s) 186
BauI CACGAG 1 cut(s) 1233
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 1 cut(s) 621
BbvI GCAGC 7 cut(s) 278, 290, 318, 689, 871, 1022, 1327
BccI CCATC 2 cut(s) 134, 970
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 1 cut(s) 807
BciVI GTATCC 1 cut(s) 763
BclI TGATCA 1 cut(s) 793
BcnI CCSGG 3 cut(s) 727, 757, 1274
BcoDI GTCTC 2 cut(s) 333, 727
BfaI CTAG 4 cut(s) 635, 935, 986, 999
BfmI CTRYAG 2 cut(s) 304, 675
BfoI RGCGCY 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 772
BfuI GTATCC 1 cut(s) 763
BisI GCNGC 7 cut(s) 267, 304, 307, 678, 885, 1036, 1341
BlpI GCTNAGC 1 cut(s) 981
BlsI GCNGC 7 cut(s) 268, 305, 308, 679, 886, 1037, 1342
Bme1390I CCNGG 4 cut(s) 727, 757, 807, 1274
Bme18I GGWCC 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 188
BmrFI CCNGG 4 cut(s) 727, 757, 807, 1274
BmrI ACTGGG 1 cut(s) 1231
BmsI GCATC 3 cut(s) 515, 1131, 1132
BmtI GCTAGC 1 cut(s) 989
BmuI ACTGGG 1 cut(s) 1231
BpiI GAAGAC 1 cut(s) 837
BpmI CTGGAG 1 cut(s) 1113
Bpu10I CCTNAGC 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 981
BpuEI CTTGAG 3 cut(s) 434, 785, 1076
BpuMI CCSGG 3 cut(s) 727, 757, 1274
BsaBI GATNNNNATC 3 cut(s) 291, 795, 1100
BsaHI GRCGYC 1 cut(s) 187
BsaI GGTCTC 2 cut(s) 333, 727
BsaJI CCNNGG 5 cut(s) 226, 321, 756, 806, 1177
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 324, 969
Bse1I ACTGG 3 cut(s) 877, 955, 1226
Bse8I GATNNNNATC 3 cut(s) 291, 795, 1100
BseBI CCWGG 1 cut(s) 807
BseDI CCNNGG 5 cut(s) 226, 321, 756, 806, 1177
BseGI GGATG 3 cut(s) 1027, 1123, 1269
BseJI GATNNNNATC 3 cut(s) 291, 795, 1100
BseLI CCNNNNNNNGG 3 cut(s) 131, 324, 969
BseMII CTCAG 3 cut(s) 531, 902, 972
BseNI ACTGG 3 cut(s) 877, 955, 1226
BseRI GAGGAG 2 cut(s) 307, 310
BseXI GCAGC 7 cut(s) 278, 290, 318, 689, 871, 1022, 1327
BsgI GTGCAG 1 cut(s) 458
Bsh1285I CGRYCG 1 cut(s) 232
BshNI GGYRCC 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 621
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 3 cut(s) 726, 756, 1274
BslFI GGGAC 2 cut(s) 604, 1147
BslI CCNNNNNNNGG 3 cut(s) 131, 324, 969
BsmAI GTCTC 2 cut(s) 333, 727
BsmFI GGGAC 2 cut(s) 604, 1147
Bso31I GGTCTC 2 cut(s) 333, 727
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 3 cut(s) 292, 793, 1237
Bsp1720I GCTNAGC 1 cut(s) 981
BspACI CCGC 2 cut(s) 20, 104
BspCNI CTCAG 3 cut(s) 532, 901, 973
BspHI TCATGA 2 cut(s) 643, 898
BspLI GGNNCC 1 cut(s) 188
BspMAI CTGCAG 2 cut(s) 308, 679
BspMI ACCTGC 1 cut(s) 772
BspOI GCTAGC 1 cut(s) 989
BspPI GGATC 1 cut(s) 1232
BspT107I GGYRCC 1 cut(s) 186
BspTNI GGTCTC 2 cut(s) 333, 727
BsrI ACTGG 3 cut(s) 877, 955, 1226
BssECI CCNNGG 5 cut(s) 226, 321, 756, 806, 1177
BssMI GATC 3 cut(s) 292, 793, 1237
BssNI GRCGYC 1 cut(s) 187
BssSI CACGAG 1 cut(s) 1233
BssT1I CCWWGG 1 cut(s) 1177
Bst2BI CACGAG 1 cut(s) 1233
Bst2UI CCWGG 1 cut(s) 807
Bst6I CTCTTC 1 cut(s) 1257
BstACI GRCGYC 1 cut(s) 187
BstC8I GCNNGC 3 cut(s) 663, 667, 987
BstDEI CTNAG 5 cut(s) 117, 540, 888, 981, 990
BstF5I GGATG 3 cut(s) 1027, 1123, 1269
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 2 cut(s) 189, 370
BstKTI GATC 3 cut(s) 295, 796, 1240
BstMAI GTCTC 2 cut(s) 333, 727
BstMBI GATC 3 cut(s) 292, 793, 1237
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 3 cut(s) 231, 266, 986
BstNI CCWGG 1 cut(s) 807
BstSCI CCNGG 4 cut(s) 725, 755, 805, 1272
BstSFI CTRYAG 2 cut(s) 304, 675
BstV1I GCAGC 7 cut(s) 278, 290, 318, 689, 871, 1022, 1327
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 1 cut(s) 1237
BstXI CCANNNNNNTGG 1 cut(s) 1248
BstYI RGATCY 1 cut(s) 1237
BsuI GTATCC 1 cut(s) 763
BtgZI GCGATG 1 cut(s) 989
BtsCI GGATG 3 cut(s) 1027, 1123, 1269
BtsIMutI CAGTG 1 cut(s) 1059
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 3 cut(s) 663, 667, 987
CciI TCATGA 2 cut(s) 643, 898
CfoI GCGC 2 cut(s) 189, 370
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 1 cut(s) 1087
CviQI GTAC 1 cut(s) 1087
DdeI CTNAG 5 cut(s) 117, 540, 888, 981, 990
DinI GGCGCC 1 cut(s) 188
DpnI GATC 3 cut(s) 294, 795, 1239
DpnII GATC 3 cut(s) 292, 793, 1237
DraI TTTAAA 1 cut(s) 1171
Eam1104I CTCTTC 1 cut(s) 1257
EarI CTCTTC 1 cut(s) 1257
Eco130I CCWWGG 1 cut(s) 1177
Eco31I GGTCTC 2 cut(s) 333, 727
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 805
EcoT14I CCWWGG 1 cut(s) 1177
EgeI GGCGCC 1 cut(s) 188
EheI GGCGCC 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 1177
FalI AAGNNNNNCTT 2 cut(s) 1098, 1130
FaqI GGGAC 2 cut(s) 604, 1147
FauI CCCGC 1 cut(s) 111
FbaI TGATCA 1 cut(s) 793
Fnu4HI GCNGC 7 cut(s) 267, 304, 307, 678, 885, 1036, 1341
FokI GGATG 3 cut(s) 1034, 1110, 1256
Fsp4HI GCNGC 7 cut(s) 267, 304, 307, 678, 885, 1036, 1341
FspBI CTAG 4 cut(s) 635, 935, 986, 999
GlaI GCGC 2 cut(s) 188, 369
GluI GCNGC 7 cut(s) 267, 304, 307, 678, 885, 1036, 1341
GsuI CTGGAG 1 cut(s) 1113
HaeII RGCGCY 1 cut(s) 190
HapII CCGG 3 cut(s) 726, 756, 1274
HhaI GCGC 2 cut(s) 189, 370
Hin1I GRCGYC 1 cut(s) 187
Hin6I GCGC 2 cut(s) 187, 368
HinP1I GCGC 2 cut(s) 187, 368
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 3 cut(s) 485, 500, 1154
HpaII CCGG 3 cut(s) 726, 756, 1274
HphI GGTGA 5 cut(s) 159, 164, 331, 389, 796
Hpy166II GTNNAC 1 cut(s) 925
Hpy188III TCNNGA 9 cut(s) 193, 280, 330, 589, 644, 899, 1093, 1233, 1241
Hpy8I GTNNAC 1 cut(s) 925
HpyAV CCTTC 3 cut(s) 56, 702, 856
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 8 cut(s) 152, 306, 439, 506, 665, 677, 956, 1123
HpyF10VI GCNNNNNNNGC 3 cut(s) 231, 266, 986
HpyF3I CTNAG 5 cut(s) 117, 540, 888, 981, 990
HpySE526I ACGT 1 cut(s) 200
Hsp92I GRCGYC 1 cut(s) 187
HspAI GCGC 2 cut(s) 187, 368
KasI GGCGCC 1 cut(s) 186
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 3 cut(s) 292, 793, 1237
LmnI GCTCC 5 cut(s) 222, 865, 881, 1138, 1337
Lsp1109I GCAGC 7 cut(s) 278, 290, 318, 689, 871, 1022, 1327
LweI GCATC 3 cut(s) 515, 1131, 1132
MaeI CTAG 4 cut(s) 635, 935, 986, 999
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 1057
MalI GATC 3 cut(s) 294, 795, 1239
MboI GATC 3 cut(s) 292, 793, 1237
MboII GAAGA 7 cut(s) 41, 473, 476, 501, 842, 1021, 1274
MflI RGATCY 1 cut(s) 1237
MhlI GDGCHC 1 cut(s) 621
Mly113I GGCGCC 1 cut(s) 187
MlyI GAGTC 2 cut(s) 479, 1163
MmeI TCCRAC 1 cut(s) 1021
MroXI GAANNNNTTC 1 cut(s) 1113
MseI TTAA 5 cut(s) 531, 852, 1170, 1205, 1215
MspA1I CMGCKG 2 cut(s) 260, 680
MspI CCGG 3 cut(s) 726, 756, 1274
MspR9I CCNGG 4 cut(s) 727, 757, 807, 1274
MvaI CCWGG 1 cut(s) 807
MwoI GCNNNNNNNGC 3 cut(s) 231, 266, 986
NarI GGCGCC 1 cut(s) 187
NciI CCSGG 3 cut(s) 727, 757, 1274
NdeII GATC 3 cut(s) 292, 793, 1237
NheI GCTAGC 1 cut(s) 985
NlaIV GGNNCC 1 cut(s) 188
NmuCI GTSAC 1 cut(s) 1057
PagI TCATGA 2 cut(s) 643, 898
PaqCI CACCTGC 1 cut(s) 772
PdmI GAANNNNTTC 1 cut(s) 1113
PfeI GAWTC 1 cut(s) 500
PflFI GACNNNGTC 1 cut(s) 719
PflMI CCANNNNNTGG 1 cut(s) 969
PfoI TCCNGGA 2 cut(s) 725, 1272
PkrI GCNGC 7 cut(s) 268, 305, 308, 679, 886, 1037, 1342
PleI GAGTC 2 cut(s) 479, 1162
PluTI GGCGCC 1 cut(s) 190
PpsI GAGTC 2 cut(s) 479, 1162
PshBI ATTAAT 2 cut(s) 1205, 1215
Psp1406I AACGTT 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 805
PspGI CCWGG 1 cut(s) 805
PspN4I GGNNCC 1 cut(s) 188
PspPI GGNCC 1 cut(s) 206
PstI CTGCAG 2 cut(s) 308, 679
PsuI RGATCY 1 cut(s) 1237
PsyI GACNNNGTC 1 cut(s) 719
PvuII CAGCTG 2 cut(s) 260, 680
RsaI GTAC 1 cut(s) 1088
RsaNI GTAC 1 cut(s) 1087
SaqAI TTAA 5 cut(s) 531, 852, 1170, 1205, 1215
SatI GCNGC 7 cut(s) 267, 304, 307, 678, 885, 1036, 1341
Sau3AI GATC 3 cut(s) 292, 793, 1237
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 2 cut(s) 479, 1163
ScrFI CCNGG 4 cut(s) 727, 757, 807, 1274
SduI GDGCHC 1 cut(s) 621
SfaNI GCATC 3 cut(s) 515, 1131, 1132
SfcI CTRYAG 2 cut(s) 304, 675
SfoI GGCGCC 1 cut(s) 188
SinI GGWCC 1 cut(s) 206
SmlI CTYRAG 3 cut(s) 413, 800, 1091
SmoI CTYRAG 3 cut(s) 413, 800, 1091
SsiI CCGC 2 cut(s) 20, 104
SspDI GGCGCC 1 cut(s) 186
SspI AATATT 1 cut(s) 535
SspMI CTAG 4 cut(s) 635, 935, 986, 999
StyD4I CCNGG 4 cut(s) 725, 755, 805, 1272
StyI CCWWGG 1 cut(s) 1177
TaiI ACGT 1 cut(s) 203
TaqI TCGA 6 cut(s) 194, 331, 600, 615, 1117, 1299
TaqII GACCGA 1 cut(s) 218
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 5 cut(s) 531, 852, 1170, 1205, 1215
Tru9I TTAA 5 cut(s) 531, 852, 1170, 1205, 1215
TscAI CASTG 1 cut(s) 1066
TseFI GTSAC 1 cut(s) 1057
TseI GCWGC 7 cut(s) 266, 303, 306, 677, 884, 1035, 1340
Tsp45I GTSAC 1 cut(s) 1057
TspGWI ACGGA 1 cut(s) 1088
TspRI CASTG 1 cut(s) 1066
Tth111I GACNNNGTC 1 cut(s) 719
Van91I CCANNNNNTGG 1 cut(s) 969
VpaK11BI GGWCC 1 cut(s) 206
VspI ATTAAT 2 cut(s) 1205, 1215
XapI RAATTY 1 cut(s) 1244
XmnI GAANNNNTTC 1 cut(s) 1113
XspI CTAG 4 cut(s) 635, 935, 986, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.